Pseudomonas aeruginosa UCBPP-PA14

Summary

Alias: Pseudomonas aeruginosa str. UCBPP-PA14

Top Publications

  1. pmc Pseudomonas aeruginosa killing of Caenorhabditis elegans used to identify P. aeruginosa virulence factors
    M W Tan
    Department of Genetics, Harvard Medical School and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 96:2408-13. 1999
  2. pmc Immigration of susceptible hosts triggers the evolution of alternative parasite defence strategies
    Hélène Chabas
    ESI, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall TR10 9FE, UK
    Proc Biol Sci 283:. 2016
  3. pmc Crystallization and preliminary X-ray diffraction analysis of a DING protein from Pseudomonas aeruginosa PA14
    Ahmed Djeghader
    Aix Marseille Universite, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 5, France
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69:425-9. 2013
  4. ncbi Evidence for past integration of IncP-1 plasmids into bacterial chromosomes
    Chung Min Chiu
    School of Bioscience, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    FEMS Microbiol Lett 241:163-9. 2004
  5. pmc Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa PelD
    Lindsey S Marmont
    Research Institute, Hospital for Sick Children, Toronto, ON, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:181-4. 2012
  6. pmc Pseudomonas aeruginosa RhlR is required to neutralize the cellular immune response in a Drosophila melanogaster oral infection model
    Stefanie Limmer
    Equipe Fondation Recherche Médicale, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, Universite de Strasbourg, F67084 Strasbourg Cedex, France
    Proc Natl Acad Sci U S A 108:17378-83. 2011
  7. pmc Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance
    Li Zhang
    Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Room 4125, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5
    J Bacteriol 193:5510-3. 2011
  8. pmc Role of exopolysaccharides in Pseudomonas aeruginosa biofilm formation and architecture
    Aamir Ghafoor
    Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand
    Appl Environ Microbiol 77:5238-46. 2011
  9. pmc Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa
    James F Parsons
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Biochemistry 46:1821-8. 2007
  10. ncbi Crystallization and preliminary x-ray investigation of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens
    J Drenth
    J Biol Chem 250:5268-9. 1975

Research Grants

Patents

  1. METHODS AND COMPOSITIONS FOR CONTROLLING GENE EXPRESSION BY RNA PROCESSING
  2. Plants with increased yield (nue)
  3. Process for the production of a fine chemical
  4. Proteins associated with abiotic stress response and homologs
  5. RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES
  6. Yield increase in plants overexpressing the accdp genes
  7. Proteins associated with abiotic stress response and homologs
  8. Process for the control of production of fine chemicals
  9. Process for the production of fine chemicals
  10. Plants with increased tolerance and/or resistance to environmental stress and increased biomass production

Scientific Experts

  • Martin Welch
  • Rien Hoge
  • David A R Sanders
  • James Parsons
  • Alan P Lewis
  • A D Nikulin
  • Andreas Kreusch
  • Valentina Molteni
  • Joerg Overhage
  • Harry H Low
  • Yannick Lequette
  • M A Valvano
  • David Cobessi
  • J Hasegawa
  • Matthew K Higgins
  • Catherine Regni
  • Yun Jeong Heo
  • You Hee Cho
  • Seol Hee Kim
  • Hélène Chabas
  • C D Stout
  • Ahmed Djeghader
  • Lindsey S Marmont
  • Hye Jin Yoon
  • Aamir Ghafoor
  • Stefanie Limmer
  • Li Zhang
  • Pol Nadal Jimenez
  • Ryan C Hunter
  • Gregory C Palmer
  • Bo Young Lee
  • Rashmi Gupta
  • Véronique Viarre
  • Sonja Storbeck
  • Michaela Wimmerova
  • Gianlucca G Nicastro
  • Chris L Rife
  • In Young Chung
  • Patricia L Taylor
  • Matthew J Wargo
  • Aude Echalier
  • B K Burgess
  • Young Seok Choi
  • Eric J Drake
  • Gregor Hagelueken
  • An Wang
  • Changjiang Dong
  • Darci R Block
  • Alessandro Borgia
  • Dirk W Heinz
  • Wolf Dieter Schubert
  • Santiago Ramon-Maiques
  • Angela Wilks
  • Etienne H Meyer
  • Lesa J Beamer
  • Chris Rife
  • Frederic Frère
  • F A Armstrong
  • Ty A Gould
  • Laurence Serre
  • Se Won Suh
  • Chung Min Chiu
  • Rachel E Rigsby
  • Jung Min Choi
  • Jonathan Friedman
  • Raul Camba
  • Edze R Westra
  • Richard N Armstrong
  • Hyung Jun Ahn
  • Stineke van Houte
  • Nina Molin Høyland-Kroghsbo
  • Ehmke Pohl
  • Marcia E Newcomer
  • Angus Buckling
  • Suzanne C Cordell
  • C David Stout
  • Barbara K Burgess
  • Fraser A Armstrong
  • Yean Sung Jung
  • Kaisheng Chen
  • Laura E Naught
  • B Shen
  • Andrew Suh
  • Ken Scott
  • Daniel Gonzalez
  • Eric Chabriere
  • Guillaume Gotthard
  • Mikael Elias
  • John C Whitney
  • Howard Robinson

Detail Information

Publications81

  1. pmc Pseudomonas aeruginosa killing of Caenorhabditis elegans used to identify P. aeruginosa virulence factors
    M W Tan
    Department of Genetics, Harvard Medical School and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 96:2408-13. 1999
    ..These data indicate that the killing of C. elegans by P. aeruginosa can be exploited to identify novel P. aeruginosa virulence factors important for mammalian pathogenesis...
  2. pmc Immigration of susceptible hosts triggers the evolution of alternative parasite defence strategies
    Hélène Chabas
    ESI, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall TR10 9FE, UK
    Proc Biol Sci 283:. 2016
    ..This effect emerges from an increase in the force of infection, which tips the balance from CRISPR to surface modification-based defence owing to the induced and fixed fitness costs associated with these mechanisms, respectively. ..
  3. pmc Crystallization and preliminary X-ray diffraction analysis of a DING protein from Pseudomonas aeruginosa PA14
    Ahmed Djeghader
    Aix Marseille Universite, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 5, France
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69:425-9. 2013
    ..Here, the expression, purification and crystallization of PA14DING and the collection of X-ray data to 1.9 Å resolution are reported...
  4. ncbi Evidence for past integration of IncP-1 plasmids into bacterial chromosomes
    Chung Min Chiu
    School of Bioscience, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    FEMS Microbiol Lett 241:163-9. 2004
    ..These data provide evidence for multiple past integration events of IncP-1 plasmids into bacterial chromosomes and provide new evidence for IncP-1 plasmids being important elements in gene mobility...
  5. pmc Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa PelD
    Lindsey S Marmont
    Research Institute, Hospital for Sick Children, Toronto, ON, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:181-4. 2012
    ..2 Å. On the basis of the Matthews coefficient (V(M) = 2.29 Å(3) Da(-1)), it was estimated that two molecules are present in the asymmetric unit...
  6. pmc Pseudomonas aeruginosa RhlR is required to neutralize the cellular immune response in a Drosophila melanogaster oral infection model
    Stefanie Limmer
    Equipe Fondation Recherche Médicale, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, Universite de Strasbourg, F67084 Strasbourg Cedex, France
    Proc Natl Acad Sci U S A 108:17378-83. 2011
    ..These results illustrate the power of studying infection from the dual perspective of host and pathogen by revealing that RhlR plays a more complex role during pathogenesis than previously appreciated...
  7. pmc Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance
    Li Zhang
    Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Room 4125, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5
    J Bacteriol 193:5510-3. 2011
    ..We showed that tssC1 expression is induced in biofilms and confirmed that tssC1 is required for T6S...
  8. pmc Role of exopolysaccharides in Pseudomonas aeruginosa biofilm formation and architecture
    Aamir Ghafoor
    Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand
    Appl Environ Microbiol 77:5238-46. 2011
    ..Overall, this study demonstrated that the various exopolysaccharides and eDNA interactively contribute to the biofilm architecture of P. aeruginosa...
  9. pmc Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa
    James F Parsons
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Biochemistry 46:1821-8. 2007
    ..This observation suggests that a mechanism has evolved in P. aeruginosa that ensures efficient production of pyocyanin via the prevention of the formation and release of an unstable and potentially deleterious intermediate...
  10. ncbi Crystallization and preliminary x-ray investigation of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens
    J Drenth
    J Biol Chem 250:5268-9. 1975
    ..5, 39% saturated ammonium sulfate, and 1mM p-hydroxybenzoate. The space group is C2221 with 8 molecules/unit cell. When p-hydroxybenzoate is removed, the diffraction pattern and the cell dimensions change...
  11. pmc RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation
    Changjiang Dong
    Centre for Biomolecular Sciences, The University, St Andrews KY16 9ST, UK
    J Mol Biol 365:146-59. 2007
    ..Clear knowledge of the chemical structure of RmlC reaction intermediates may offer new opportunities for rational drug design...
  12. ncbi The reaction of phosphohexomutase from Pseudomonas aeruginosa: structural insights into a simple processive enzyme
    Catherine Regni
    Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, USA
    J Biol Chem 281:15564-71. 2006
    ....
  13. ncbi Unveiling a hidden folding intermediate in c-type cytochromes by protein engineering
    Alessandro Borgia
    Istituto Pasteur Fondazione Cenci Bolognetti and Istituto di Biologia e Patologia Molecolari del CNR, Dipartimento di Scienze Biochimiche, Universita di Roma La Sapienza, P le A Moro 5, 00185 Rome, Italy
    J Biol Chem 281:9331-6. 2006
    ..These results show how protein engineering, x-ray crystallography and state-of-the-art kinetics concur to unveil a folding intermediate and the structural determinants of its stability...
  14. ncbi Structural bases of feed-back control of arginine biosynthesis, revealed by the structures of two hexameric N-acetylglutamate kinases, from Thermotoga maritima and Pseudomonas aeruginosa
    Santiago Ramon-Maiques
    Instituto de Biomedicina de Valencia CSIC, Jaume Roig 11, Valencia 46010, Spain
    J Mol Biol 356:695-713. 2006
    ..These signatures are found also in the homologous arginine-inhibited enzyme NAG synthase. The findings on NAGK shed light on the structure, function and arginine inhibition of this synthase, for which a hexameric model is constructed...
  15. pmc AtCCMH, an essential component of the c-type cytochrome maturation pathway in Arabidopsis mitochondria, interacts with apocytochrome c
    Etienne H Meyer
    Institut de Biologie Moleculaire des Plantes, Centre National de la Recherche Scientifique, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Proc Natl Acad Sci U S A 102:16113-8. 2005
    ..Our results show that AtCCMH is an essential mitochondrial protein with characteristics consistent with its proposed apocytochrome c-reducing and heme lyase function...
  16. ncbi DING proteins are from Pseudomonas
    Alan P Lewis
    Bioinformatics Discovery and Analysis, GlaxoSmithKline Medicines Research Centre, Stevenage, UK
    FEMS Microbiol Lett 252:215-22. 2005
    ....
  17. ncbi Crystal structure at high resolution of ferric-pyochelin and its membrane receptor FptA from Pseudomonas aeruginosa
    David Cobessi
    Département Récepteurs et Protéines Membranaires, UMR7100 CNRS, Ecole Superieure de Biotechnologie de Strasbourg, Boulevard Sebastien Brant, 67412 Illkirch, France
    J Mol Biol 352:893-904. 2005
    ..The N-terminal TonB box is disordered in two crystal forms, and loop L8 is found to point towards the iron-pyochelin complex, suggesting that the receptor is in a transport-competent conformation...
  18. ncbi Crystal structure of the global regulatory protein CsrA from Pseudomonas putida at 2.05 A resolution reveals a new fold
    Chris Rife
    The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
    Proteins 61:449-53. 2005
  19. ncbi Crystal structure of nicotinic acid mononucleotide adenylyltransferase from Pseudomonas aeruginosa in its Apo and substrate-complexed forms reveals a fully open conformation
    Hye Jin Yoon
    Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151 742, South Korea
    J Mol Biol 351:258-65. 2005
    ..We suggest that such a conformational change likely occurs only after both substrates are simultaneously bound in the active site...
  20. ncbi Tracking the evolution of porphobilinogen synthase metal dependence in vitro
    Frederic Frère
    Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, D 38106 Braunschweig, Germany
    J Mol Biol 345:1059-70. 2005
    ....
  21. ncbi Phosphonoformate: a minimal transition state analogue inhibitor of the fosfomycin resistance protein, FosA
    Rachel E Rigsby
    Department of Chemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 43:13666-73. 2004
    ..Taken together, these data suggest that the FosA-catalyzed reaction has a very early transition state and phosphonoformate acts as a minimal transition state analogue inhibitor...
  22. ncbi The crystal structure of ZapA and its modulation of FtsZ polymerisation
    Harry H Low
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 341:839-52. 2004
    ..Given the symmetry of these ZapA oligomers and the polar nature of FtsZ filaments, the structure of ZapA carries novel implications for the inherent architecture of the Z-ring in vivo...
  23. pmc Structure of the periplasmic component of a bacterial drug efflux pump
    Matthew K Higgins
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 101:9994-9. 2004
    ..On the basis of the structure and packing, we suggest a model for the key periplasmic interaction between the outer membrane channel and the adaptor protein in the assembled drug efflux pump...
  24. doi Importance of oligomerisation on Pseudomonas aeruginosaLectin-II binding affinity. In silico and in vitro mutagenesis
    Michaela Wimmerova
    National Centre for Biomolecular Research, Masaryk University, Faculty Science, Kotlarska 2, 611 37 Brno, Czech Republic
    J Mol Model 15:673-9. 2009
    ..It implies that the PA-IIL saccharide binding affinity is influenced by the dimerization of the lectin. A detailed analysis of computational data confirms the key role of electrostatic interactions in the PA-IIL/saccharide binding...
  25. pmc A putative ABC transporter, hatABCDE, is among molecular determinants of pyomelanin production in Pseudomonas aeruginosa
    Ryan C Hunter
    Division of Biological Sciences, California Institute of Technology, Pasadena, CA 91125, USA
    J Bacteriol 192:5962-71. 2010
    ..Based on their involvement in homogentisic acid transport, we rename the genes of this operon hatABCDE...
  26. pmc Characterization of the Pseudomonas aeruginosa transcriptional response to phenylalanine and tyrosine
    Gregory C Palmer
    Section of Molecular Genetics and Microbiology, Institute of Cell and Molecular Biology, The University of Texas at Austin, 1 University Station, A5000, Austin, TX 78712, USA
    J Bacteriol 192:2722-8. 2010
    ..Our work characterizes a transcriptional regulator of catabolic genes induced during P. aeruginosa growth in CF sputum...
  27. ncbi IscR modulates catalase A (KatA) activity, peroxide resistance and full virulence of Pseudomonas aeruginosa PA14
    Seol Hee Kim
    Department of Life Science, Sogang University, Seoul 121 742, Korea
    J Microbiol Biotechnol 19:1520-6. 2009
    ..These results suggest that the requirement of IscR in P. aeruginosa is related to the proper activity of KatA, which is crucial for peroxide resistance and full virulence of this bacterium...
  28. pmc The major catalase gene (katA) of Pseudomonas aeruginosa PA14 is under both positive and negative control of the global transactivator OxyR in response to hydrogen peroxide
    Yun Jeong Heo
    Department of Life Science, Sogang University, Mapo gu, Seoul 121 742, South Korea
    J Bacteriol 192:381-90. 2010
    ..aeruginosa OxyR is a bona fide transcriptional regulator of the katA gene, sensing H(2)O(2) based on the conserved Cys residues, involving more than one oxidation as well as activation state in vivo...
  29. doi Pseudomonas aeruginosa PA14 cupD transcription is activated by the RcsB response regulator, but repressed by its putative cognate sensor RcsC
    Gianlucca G Nicastro
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
    FEMS Microbiol Lett 301:115-23. 2009
    ..Comparison of P. aeruginosa and Escherichia coli Rcs may provide insights into how similar systems can be used by different bacteria to control gene expression and to adapt to various environmental conditions...
  30. pmc HxcQ liposecretin is self-piloted to the outer membrane by its N-terminal lipid anchor
    Véronique Viarre
    Laboratoire d Ingénierie des Systèmes Macromoléculaires UPR 9027, CNRS, Institut de Microbiologie de la Mediterranee, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 284:33815-23. 2009
    ..Altogether, our results reveal an original and unprecedented pathway for secretin transport to the OM...
  31. doi The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase required for heme d(1) biosynthesis
    Sonja Storbeck
    Institute of Microbiology, Technische Universitat Braunschweig, Germany
    FEBS J 276:5973-82. 2009
    ..However, bacterial growth of the nirE mutant was observed when cobA was constitutively expressed by a complementing plasmid, underscoring the special requirement of NirE for heme d(1) biosynthesis...
  32. pmc GidA posttranscriptionally regulates rhl quorum sensing in Pseudomonas aeruginosa
    Rashmi Gupta
    Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331, USA
    J Bacteriol 191:5785-92. 2009
    ..Taken together, these results indicate that GidA selectively controls QS gene expression posttranscriptionally via RhlR-dependent and -independent pathways...
  33. ncbi The 1.8 A crystal structure of PA2412, an MbtH-like protein from the pyoverdine cluster of Pseudomonas aeruginosa
    Eric J Drake
    Hauptman Woodward Medical Research Institute, State University of New York at Buffalo, 700 Ellicott Street, Buffalo, NY 14203, USA
    J Biol Chem 282:20425-34. 2007
    ..The PA2412 deletion strain is able to use exogenously produced pyoverdine, showing that there is no defect in the uptake or utilization of the iron-pyoverdine complex...
  34. doi Redox-linked structural changes associated with the formation of a catalytically competent form of the diheme cytochrome c peroxidase from Pseudomonas aeruginosa
    Aude Echalier
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    Biochemistry 47:1947-56. 2008
    ..Peterson, J., Gadsby, P., Greenwood, C., and Thomson, A. (1985) Biochem. J. 230, 227-237) correspond to the Ca2+-loaded and -depleted forms of the enzyme...
  35. ncbi Structure and function of sedoheptulose-7-phosphate isomerase, a critical enzyme for lipopolysaccharide biosynthesis and a target for antibiotic adjuvants
    Patricia L Taylor
    Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada
    J Biol Chem 283:2835-45. 2008
    ..We postulate GmhA acts through an enediol-intermediate isomerase mechanism...
  36. ncbi Identification of two heme-binding sites in the cytoplasmic heme-trafficking protein PhuS from Pseudomonas aeruginosa and their relevance to function
    Darci R Block
    Department of Chemistry, Biochemistry and Molecular Biology, North Dakota State University, 1231 Albrecht Avenue, Fargo, North Dakota 58105, USA
    Biochemistry 46:14391-402. 2007
    ..The multiple heme-bound states and their sensitivity to pH suggest the possibility that these cytoplasmic heme-binding proteins have multiple functions that are toggled by variations in intracellular conditions...
  37. pmc Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism
    Matthew J Wargo
    Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    J Bacteriol 190:2690-9. 2008
    ..These studies provided important insight into both the mechanism and the regulation of the catabolism of GB in P. aeruginosa...
  38. ncbi Crystal structure of the oxidized form of the periplasmic mercury-binding protein MerP from Ralstonia metallidurans CH34
    Laurence Serre
    Institut de Biologie Structurale, UMR 5075 CNRS CEA UJF IBS LPM, 41 Avenue Jules Horowitz, 38027 Grenoble Cedex 01, France
    J Mol Biol 339:161-71. 2004
    ..We propose a possible mechanism of mercury transfer between two CXXC motifs based on a transient bi-coordinated mercury intermediate...
  39. ncbi Biochemical and structural characterization of Pseudomonas aeruginosa Bfd and FPR: ferredoxin NADP+ reductase and not ferredoxin is the redox partner of heme oxygenase under iron-starvation conditions
    An Wang
    Ralph N Adams Institute for Bioanalytical Chemistry and Department of Chemistry, University of Kansas, Multidisciplinary Research Building, 2030 Becker Drive, Room 220 E, Lawrence, Kansas 66047, USA
    Biochemistry 46:12198-211. 2007
    ..Hence, findings reported herein extend the range of redox partners recognized by the fold of pa-FPR to include a heme oxygenase (pa-HO)...
  40. pmc Crystal structure of the electron transfer complex rubredoxin rubredoxin reductase of Pseudomonas aeruginosa
    Gregor Hagelueken
    Molecular Host Pathogen Interactions, Division of Structural Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D 38124 Braunschweig, Germany
    Proc Natl Acad Sci U S A 104:12276-81. 2007
    ..Only a small number of direct interactions govern mutual recognition of RdxR and Rdx, corroborating the transient nature of the complex. The shortest distance between the redox centers is observed to be 6.2 A...
  41. pmc Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: cysteine ligation of the [4Fe-4S] cluster with protein rearrangement is preferred over serine ligation
    B Shen
    Department of Molecular Biology and Biochemistry, University of California, Irvine 92717, USA
    Proc Natl Acad Sci U S A 92:10064-8. 1995
    ..In the C20S protein the [4Fe-4S] cluster has altered stability and redox properties relative to either C20A or the native protein...
  42. ncbi High resolution crystal structure of a Mg2+-dependent porphobilinogen synthase
    N Frankenberg
    Albert Ludwigs Universitat, Albertstrasse 21, Freiburg, D 79104, Germany
    J Mol Biol 289:591-602. 1999
    ..We conclude that the observed differences in the active sites of both monomers might be induced by Mg2+-binding to this remote site and propose a structure-based mechanism for this allosteric Mg2+in rate enhancement...
  43. ncbi Structural basis for novel delta-regioselective heme oxygenation in the opportunistic pathogen Pseudomonas aeruginosa
    Jonathan Friedman
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    Biochemistry 43:5239-45. 2004
    ....
  44. ncbi Lys42 and Ser42 variants of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens reveal that Arg42 is essential for NADPH binding
    M H Eppink
    Department of Biomolecular Sciences, Wageningen Agricultural University, The Netherlands
    Eur J Biochem 253:194-201. 1998
    ..In spite of this, the Arg42 variants fully couple enzyme reduction to substrate hydroxylation. Sequence-comparison studies suggest that Arg42 is involved in binding of the 2'-phosphoadenosine moiety of NADPH...
  45. ncbi Three-dimensional structure of lipoamide dehydrogenase from Pseudomonas fluorescens at 2.8 A resolution. Analysis of redox and thermostability properties
    A Mattevi
    BIOSON Research Institute, Department of Chemistry, University of Groningen, The Netherlands
    J Mol Biol 230:1200-15. 1993
    ....
  46. pmc Antitermination of amidase expression in Pseudomonas aeruginosa is controlled by a novel cytoplasmic amide-binding protein
    S A Wilson
    Biomolecular Structure Group, University College London, UK
    EMBO J 12:3637-42. 1993
    ..Sequence analysis techniques suggest that AmiC is a member of the structural family of periplasmic binding proteins, but has a distinct and novel cytoplasmic role...
  47. ncbi Azotobacter vinelandii ferredoxin I. Aspartate 15 facilitates proton transfer to the reduced [3Fe-4S] cluster
    B Shen
    Department of Molecular Biology and Biochemistry, University of California, Irvine 92717
    J Biol Chem 268:25928-39. 1993
    ..Overproduction of D15N FdI, but not native FdI, in A. vinelandii has a negative effect on the growth rate of the organism, suggesting that the rate of protonation or deprotonation of the [3Fe-4S]0 cluster may be important in vivo...
  48. ncbi Crystal structures of oxidized and reduced Azotobacter vinelandii ferredoxin at pH 8 and 6
    C D Stout
    Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037
    J Biol Chem 268:25920-7. 1993
    ..1993).(abstract truncated at 400 words)..
  49. pmc Crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified FAD present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin
    W J van Berkel
    Department of Biochemistry, Agricultural University, Wageningen, The Netherlands
    Protein Sci 3:2245-53. 1994
    ..7 mol of hydrogen peroxide per mol NADPH oxidized. It is proposed that flavin motion in p-hydroxybenzoate hydroxylase is important for efficient reduction and that the flavin "out" conformation is associated with the oxidase activity...
  50. ncbi Characterization of the Escherichia coli CcmH protein reveals new insights into the redox pathway required for cytochrome c maturation
    R A Fabianek
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    Arch Microbiol 171:92-100. 1999
    ..We propose a model for the reaction sequence in which CcmH keeps the heme binding site of apocytochrome c in a reduced form for subsequent heme ligation...
  51. ncbi p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens. 1. Completion of the elucidation of the primary structure
    J Hofsteenge
    Eur J Biochem 133:91-108. 1983
    ..An important tool in the analysis of the amino acid sequence of peptide CB1 was the proteinase Lys-C from Lysobacter enzymogenes, which preferentially cleaves at lysine residues...
  52. ncbi p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens. 2. Fitting of the amino-acid sequence to the tertiary structure
    W J Weijer
    Eur J Biochem 133:109-18. 1983
    ..In the center of one of the largely hydrophobic contact areas between the subunits, a cluster of six aromatic amino acids was found...
  53. ncbi Structure of cytochrome c551 from Pseudomonas aeruginosa refined at 1.6 A resolution and comparison of the two redox forms
    Y Matsuura
    J Mol Biol 156:389-409. 1982
  54. ncbi Crystal structure of p-hydroxybenzoate hydroxylase complexed with its reaction product 3,4-dihydroxybenzoate
    H A Schreuder
    Laboratory of Chemical Physics, University of Groningen, The Netherlands
    J Mol Biol 199:637-48. 1988
    ....
  55. ncbi Sequential 1H NMR assignments of iron(II) cytochrome c551 from Pseudomonas aeruginosa
    D J Detlefsen
    Department of Chemistry, University of Michigan, Willard H Dow Laboratory, Ann Arbor 48109
    Biochemistry 29:9377-86. 1990
    ..For one aromatic side chain (Tyr 27) that is close to the heme group we found a transition from hindered ring rotation at low temperature to rapid rotation at high temperature...
  56. pmc Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: [Fe-S] cluster-driven protein rearrangement
    A E Martin
    Department of Molecular Biology and Biochemistry, University of California, Irvine 92717
    Proc Natl Acad Sci U S A 87:598-602. 1990
    ..The new [4Fe-4S] cluster obtains its fourth ligand from Cys-24, a free cysteine in the native structure. The formation of this [4Fe-4S] cluster drives rearrangement of the protein structure...
  57. ncbi Refined crystal structure of lipoamide dehydrogenase from Azotobacter vinelandii at 2.2 A resolution. A comparison with the structure of glutathione reductase
    A Mattevi
    Department of Chemistry, University of Groningen, The Netherlands
    J Mol Biol 220:975-94. 1991
    ..In spite of all these changes at the tertiary and quaternary level the active sites of the enzymes, which occur at the dimer interface, appear to be remarkably similar.(ABSTRACT TRUNCATED AT 400 WORDS)..
  58. ncbi Crystal structure of the reduced form of p-hydroxybenzoate hydroxylase refined at 2.3 A resolution
    H A Schreuder
    BIOSON Research Institute, University of Groningen, The Netherlands
    Proteins 14:178-90. 1992
    ..The position of the substrate is virtually unaltered with respect to its position in the oxidized enzyme. No trace of a bound NADP+ or NADPH molecule was found...
  59. ncbi Alteration of the reduction potential of the [4Fe-4S](2+/+) cluster of Azotobacter vinelandii ferredoxin I
    K Chen
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    J Biol Chem 274:36479-87. 1999
    ..Subsequent Protein Dipole Langevin Dipole calculations (using the actual I40N x-ray structures) did quite accurately predict the observed change in E(0')...
  60. ncbi Probing the functional importance of the hexameric ring structure of RNase PH
    Jung Min Choi
    National Creative Research Initiative Center for Structural Biology and Department of Life Science, Pohang University of Science and Technology, Hyo ja dong, San31, Pohang, Kyungbook 790 784, South Korea
    J Biol Chem 279:755-64. 2004
    ..Our structural and mutational analyses of RNase PH demonstrate that the hexameric ring formation is a critical feature for the function of members of the RNase PH family...
  61. ncbi Mechanisms of redox-coupled proton transfer in proteins: role of the proximal proline in reactions of the [3Fe-4S] cluster in Azotobacter vinelandii ferredoxin I
    Raul Camba
    Department of Chemistry, Oxford University, South Parks Road, Oxford, OX1 3QR, England
    Biochemistry 42:10589-99. 2003
    ..The electrostatic coupling is crucial for maintaining the efficiency of proton transfer both to and from the cluster, over a range of pH values...
  62. pmc Crystal structure of the SOS cell division inhibitor SulA and in complex with FtsZ
    Suzanne C Cordell
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Proc Natl Acad Sci U S A 100:7889-94. 2003
    ..Instead, SulA binds the T7 loop surface of FtsZ, opposite the nucleotide-binding site, blocking polymer formation. These findings explain why GTP hydrolysis and polymer turnover are required for SulA inhibition...
  63. ncbi Architecture of a protein central to iron homeostasis: crystal structure and spectroscopic analysis of the ferric uptake regulator
    Ehmke Pohl
    European Molecular Biology Laboratory, Hamburg Outstation, Notkestr 85, D 22603 Hamburg, Germany
    Mol Microbiol 47:903-15. 2003
    ..The combination of these complementary techniques enables us to present a model for the activation and DNA binding of the Fur protein...
  64. ncbi Crystal structure of a genomically encoded fosfomycin resistance protein (FosA) at 1.19 A resolution by MAD phasing off the L-III edge of Tl(+)
    Chris L Rife
    Departments of Biochemistry and Chemistry, Vanderbilt University, Nashville, Tennessee 37232 0146, USA
    J Am Chem Soc 124:11001-3. 2002
    ..The K+ ion located 6.5 A from the Mn(II) appears to help orient the substrate for nucleophilic attack...
  65. ncbi Allosterism and cooperativity in Pseudomonas aeruginosa GDP-mannose dehydrogenase
    Laura E Naught
    Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, USA
    Biochemistry 41:9637-45. 2002
    ....
  66. ncbi Structural insights into the hydrolysis of cellular nitric oxide synthase inhibitors by dimethylarginine dimethylaminohydrolase
    J Murray-Rust
    School of Crystallography, Birkbeck, Malet Street, London WC1E 7HX, UK
    Nat Struct Biol 8:679-83. 2001
    ..The DDAH structure will form a basis for the rational design of selective inhibitors, which are of potential use in modulating NO synthase activity in pathological settings...
  67. ncbi Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1
    U Strych
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 5513, USA
    Curr Microbiol 41:290-4. 2000
    ..Both gene products were purified to electrophoretic homogeneity, the enzymes were characterized biochemically, and preliminary crystals were obtained...
  68. ncbi Selected mutations in a mesophilic cytochrome c confer the stability of a thermophilic counterpart
    J Hasegawa
    Daiichi Pharmaceutical Co, Ltd, Edogawa ku, Tokyo 134 8630, Japan
    J Biol Chem 275:37824-8. 2000
    ..Our results provide a novel example of protein stabilization in that limited amino acid substitutions can confer the overall stability of a natural highly thermophilic protein upon a mesophilic molecule...
  69. ncbi Oxidized and reduced Azotobacter vinelandii ferredoxin I at 1.4 A resolution: conformational change of surface residues without significant change in the [3Fe-4S]+/0 cluster
    C G Schipke
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037 1093, USA
    Biochemistry 38:8228-39. 1999
    ..These structural changes imply a mechanism for H+ transfer to the [3Fe-4S]0 cluster in agreement with electrochemical and spectroscopic results...
  70. ncbi Crystal structures of ferredoxin variants exhibiting large changes in [Fe-S] reduction potential
    Kaisheng Chen
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    Nat Struct Biol 9:188-92. 2002
    ..The smaller (50--100 mV) increases observed for the neutral form are proposed to occur by directing a Hdelta+--Ndelta- dipole toward the reduced form of the cluster...
  71. doi Role of PvdQ in Pseudomonas aeruginosa virulence under iron-limiting conditions
    Pol Nadal Jimenez
    Department of Pharmaceutical Biology, University of Groningen, 9713 AV Groningen, The Netherlands
    Microbiology 156:49-59. 2010
    ....
  72. ncbi Y13C Azotobacter vinelandii ferredoxin I. A designed [Fe-S] ligand motif contains a cysteine persulfide
    M A Kemper
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697 3900, USA
    J Biol Chem 272:15620-7. 1997
    ..It is interesting to note that neither of the two free cysteines present in FdI was modified. Thus, if NifS is involved in modifying the introduced cysteine there must be specificity to the reaction...
  73. ncbi Structure of a 7Fe ferredoxin from Azotobacter vinelandii
    D Ghosh
    J Biol Chem 256:4185-92. 1981
    ..The partially refined structure at 2.5 A (3,490 reflections, 6.0 sigma(F)) has R = 35%...
  74. ncbi Identification of Pseudomonas aeruginosa genes crucial for hydrogen peroxide resistance
    Young Seok Choi
    Department of Life Science, Sogang University, Seoul 121 742, Korea
    J Microbiol Biotechnol 17:1344-52. 2007
    ..These results suggest that both IscR and OxyR are required for the optimal resistance to H2O2, which involves the expression of multiple antioxidant enzymes including KatA...
  75. ncbi Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa
    Hyung Jun Ahn
    Structural Proteomics Laboratory, School of Chemistry and Molecular Engineering, College of Natural Sciences, Seoul National University, Seoul 151742, South Korea
    J Mol Biol 328:505-15. 2003
    ..Thus, our work lowers the likelihood of the previous proposal that "domain duplication followed by exchange of a secondary structure element can be a source of such a knot in the protein structure" being correct...
  76. ncbi Structure of C42D Azotobacter vinelandii FdI. A Cys-X-X-Asp-X-X-Cys motif ligates an air-stable [4Fe-4S]2+/+ cluster
    Y S Jung
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    J Biol Chem 275:36974-83. 2000
    ..The cluster conversion was ultimately accomplished using a new electrochemical method. Hydrophobic and electrostatic interaction and the lack of Gly residues adjacent to the Asp ligand explain the remarkable stability of this cluster...
  77. ncbi Engineering of microheterogeneity-resistant p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens
    K Eschrich
    Department of Biochemistry, Agricultural University, Wageningen, The Netherlands
    FEBS Lett 277:197-9. 1990
    ..Crystals of the C116S mutant are isomorphous with the crystal form of wild-type enzyme. A difference electron density confirms the mutation made...
  78. ncbi Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI
    Ty A Gould
    Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, 4200 E Ninth Ave, Denver, CO 80262, USA
    Mol Microbiol 53:1135-46. 2004
    ..This structure and the novel explanation of AHL-synthase acyl-chain-length selectivity promise to guide the design of Pseudomonas aeruginosa-specific quorum-sensing inhibitors as antibacterial agents...
  79. ncbi Structural basis of diverse substrate recognition by the enzyme PMM/PGM from P. aeruginosa
    Catherine Regni
    Department of Biochemistry, 117 Schweitzer Hall, University of Missouri Columbia, Columbia, MO 65211, USA
    Structure 12:55-63. 2004
    ..aeruginosa...
  80. ncbi Identification of novel potent bicyclic peptide deformylase inhibitors
    Valentina Molteni
    Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA
    Bioorg Med Chem Lett 14:1477-81. 2004
    ..Many of the compounds synthesized proved to be excellent PDF-Ni inhibitors and some showed increased antibacterial activity in selected strains...
  81. ncbi Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides
    Miguel A Valvano
    Department of Microbiology and Immunology and Medicine, University of Western Ontario, London, Ontario, N6A 5C1, Canada
    Microbiology 148:1979-89. 2002

Research Grants1

  1. Biochemistry of Phenazine Production in Pseudomonas aeruginosa
    James Parsons; Fiscal Year: 2009
    ..New strategies that complement existing antimicrobial therapies clearly need to be developed to more effectively combat P. aeruginosa infections. ..

Patents18

  1. METHODS AND COMPOSITIONS FOR CONTROLLING GENE EXPRESSION BY RNA PROCESSING
    Patent Number: EP2880171-A2; Date:2015-06-10
  2. Plants with increased yield (nue)
    Patent Number: WO2010046221-A1; Date:2010-04-29
  3. Process for the production of a fine chemical
    Patent Number: WO2008034648-A1; Date:2008-03-27
  4. Proteins associated with abiotic stress response and homologs
    Patent Number: WO2007110314-A; Date:2007-10-04
  5. RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES
    Patent Number: KR1020130117753-A; Date:2013-10-28
  6. Yield increase in plants overexpressing the accdp genes
    Patent Number: WO2007011625-A2; Date:2007-01-25
  7. Proteins associated with abiotic stress response and homologs
    Patent Number: EP2221382-A2; Date:2010-08-25
  8. Process for the control of production of fine chemicals
    Patent Number: EP2199304-A1; Date:2010-06-23
  9. Process for the production of fine chemicals
    Patent Number: EP2194140-A2; Date:2010-06-09
  10. Plants with increased tolerance and/or resistance to environmental stress and increased biomass production
    Patent Number: WO2008142034-A2; Date:2008-11-27
  11. Method for producing a transgenic plant cell, a plant or a part thereof with increased resistance biotic stress
    Patent Number: WO2010037714-A1; Date:2010-04-08
  12. Plants with increased yield (lt)
    Patent Number: WO2010034672-A1; Date:2010-04-01
  13. Plants with increased yield by increasing or generating one or more activities in a plant or a part thereof
    Patent Number: WO2010020654-A2; Date:2010-02-25
  14. Process for the production of lutein
    Patent Number: EP2096177-A2; Date:2009-09-02
  15. Process for the production of a fine chemical
    Patent Number: EP2090662-A2; Date:2009-08-19
  16. Plants with increased yield and/or increased tolerance to environmental stress (iy-bm)
    Patent Number: WO2009077611-A2; Date:2009-06-25
  17. Plants with increased yield
    Patent Number: WO2009037329-A2; Date:2009-03-26
  18. Plants with increased yield
    Patent Number: WO2009037279-A1; Date:2009-03-26