Escherichia coli O157:H7 str. Sakai

Summary

Alias: Escherichia coli O157:H7 strain Sakai

Top Publications

  1. pmc Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation
    K G Jarvis
    Department of Medicine, Department of Veterans Affairs Medical Center, Baltimore, MD, USA
    Proc Natl Acad Sci U S A 92:7996-8000. 1995
  2. pmc Comparative toxicity and virulence of Escherichia coli clones expressing variant and chimeric Shiga-like toxin type II operons
    A W Paton
    Department of Microbiology, Women s and Children s Hospital, North Adelaide, South Australia
    Infect Immun 63:2450-8. 1995
  3. pmc Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7
    N T Perna
    Laboratory of Genetics, University of Wisconsin Madison, Madison Wisconsin 53706, USA
    Infect Immun 66:3810-7. 1998
  4. ncbi Escherichia coli cafA gene encodes a novel RNase, designated as RNase G, involved in processing of the 5' end of 16S rRNA
    M Wachi
    Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama, 226 8501, Japan
    Biochem Biophys Res Commun 259:483-8. 1999
  5. pmc Ancestral divergence, genome diversification, and phylogeographic variation in subpopulations of sorbitol-negative, beta-glucuronidase-negative enterohemorrhagic Escherichia coli O157
    J Kim
    Department of Food Science and Technology, University of Nebraska, Lincoln, NE 68583 0919, USA
    J Bacteriol 183:6885-97. 2001
  6. ncbi StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor
    Wyndham W Lathem
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 45:277-88. 2002
  7. ncbi Crystal structure of E. coli YhbY: a representative of a novel class of RNA binding proteins
    Gerard J Ostheimer
    Institute of Molecular Biology, 1229 University of Oregon, Eugene, OR 97403, USA
    Structure 10:1593-601. 2002
  8. pmc Characterization of the novel factor paa involved in the early steps of the adhesion mechanism of attaching and effacing Escherichia coli
    Isabelle Batisson
    Laboratoire de Biologie des Protistes, UMR 6023, Universite Blaise Pascal, Aubiere, France
    Infect Immun 71:4516-25. 2003
  9. ncbi Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic Escherichia coli effectors: Tir and EspFu/TccP
    Vlademir V Cantarelli
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Cell Microbiol 9:1782-95. 2007
  10. pmc Characterization of a novel type IV pilus locus encoded on the large plasmid of locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans
    Potjanee Srimanote
    Department of Molecular Biosciences, Adelaide University, Adelaide, South Australia 5005, Australia
    Infect Immun 70:3094-100. 2002

Research Grants

  1. Novel Approaches for the Control of Microbial Pathogens
    JOSEPH F LUTKENHAUS; Fiscal Year: 2010
  2. Chaperone Function in Iron-Sulfur Protein Folding
    LARRY VICKERY; Fiscal Year: 2008
  3. Postsynthetic Modifications of Bacterial Membrane Lipids
    John Cronan; Fiscal Year: 2006
  4. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
  5. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2010
  6. Ler control of EPEC virulence
    JAY MELLIES; Fiscal Year: 2008
  7. SIGNAL SEQUENCE RECOGNITION AND PROTEIN TARGETING
    Robert Stroud; Fiscal Year: 2007
  8. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006
  9. ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFER
    PERRY FREY; Fiscal Year: 2006
  10. Molecular Basis for Transmembrane Conduction & Signaling
    Robert Stroud; Fiscal Year: 2006

Detail Information

Publications111 found, 100 shown here

  1. pmc Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation
    K G Jarvis
    Department of Medicine, Department of Veterans Affairs Medical Center, Baltimore, MD, USA
    Proc Natl Acad Sci U S A 92:7996-8000. 1995
    ..These data suggest that the EPEC Sep proteins are components of a type III secretory apparatus necessary for the export of virulence determinants...
  2. pmc Comparative toxicity and virulence of Escherichia coli clones expressing variant and chimeric Shiga-like toxin type II operons
    A W Paton
    Department of Microbiology, Women s and Children s Hospital, North Adelaide, South Australia
    Infect Immun 63:2450-8. 1995
    ..These findings raise the possibility that naturally occurring SLT-II sequence variations may impact directly on the capacity of a given SLT-producing E. coli strain to cause disease...
  3. pmc Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7
    N T Perna
    Laboratory of Genetics, University of Wisconsin Madison, Madison Wisconsin 53706, USA
    Infect Immun 66:3810-7. 1998
    ....
  4. ncbi Escherichia coli cafA gene encodes a novel RNase, designated as RNase G, involved in processing of the 5' end of 16S rRNA
    M Wachi
    Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama, 226 8501, Japan
    Biochem Biophys Res Commun 259:483-8. 1999
    ..These results indicated that the cafA gene encodes a novel RNase responsible for processing of the 5' end of 16S rRNA...
  5. pmc Ancestral divergence, genome diversification, and phylogeographic variation in subpopulations of sorbitol-negative, beta-glucuronidase-negative enterohemorrhagic Escherichia coli O157
    J Kim
    Department of Food Science and Technology, University of Nebraska, Lincoln, NE 68583 0919, USA
    J Bacteriol 183:6885-97. 2001
    ..DNA sequence analysis of representative polyphyletic markers showed that genome diversity accrued through random drift and bacteriophage-mediated events...
  6. ncbi StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor
    Wyndham W Lathem
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 45:277-88. 2002
    ..Cleavage of C1-INH by StcE could plausibly cause localized pro-inflammatory and coagulation responses resulting in tissue damage, intestinal oedema and thrombotic abnormalities...
  7. ncbi Crystal structure of E. coli YhbY: a representative of a novel class of RNA binding proteins
    Gerard J Ostheimer
    Institute of Molecular Biology, 1229 University of Oregon, Eugene, OR 97403, USA
    Structure 10:1593-601. 2002
    ..Taken together, the evidence suggests that members of UPF0044 constitute a previously unrecognized class of RNA binding domain...
  8. pmc Characterization of the novel factor paa involved in the early steps of the adhesion mechanism of attaching and effacing Escherichia coli
    Isabelle Batisson
    Laboratoire de Biologie des Protistes, UMR 6023, Universite Blaise Pascal, Aubiere, France
    Infect Immun 71:4516-25. 2003
    ..These observations suggest that Paa contributes to the early stages of A/E E. coli virulence...
  9. ncbi Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic Escherichia coli effectors: Tir and EspFu/TccP
    Vlademir V Cantarelli
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Cell Microbiol 9:1782-95. 2007
    ..Binding of cortactin to these effectors is dependent upon tyrosine phosphorylation and a balance between tyrosine phosphorylation and dephosphorylation of cortactin is required to regulate pedestal formation by EHEC...
  10. pmc Characterization of a novel type IV pilus locus encoded on the large plasmid of locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans
    Potjanee Srimanote
    Department of Molecular Biosciences, Adelaide University, Adelaide, South Australia 5005, Australia
    Infect Immun 70:3094-100. 2002
    ..Homologues of the pO113 pil locus were present in several other LEE-negative STEC strains but not in LEE-positive STEC strains belonging to serogroup O26, O111, or O157...
  11. ncbi Solution structure of the R3H domain from human Smubp-2
    Edvards Liepinsh
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, S 17177, Stockholm, Sweden
    J Mol Biol 326:217-23. 2003
    ..A strong correlation between conservation of hydrophobic amino acids and side-chain solvent protection indicates that the structure of the Smubp-2 R3H domain is representative of R3H domains in general...
  12. ncbi Acquisition of stcE, a C1 esterase inhibitor-specific metalloprotease, during the evolution of Escherichia coli O157:H7
    Wyndham W Lathem
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin, USA
    J Infect Dis 187:1907-14. 2003
    ..coli 1 complex in a stepwise manner. These data refine the sequential model of E. coli O157:H7 evolution proposed elsewhere...
  13. ncbi Ribosome run through of the termination codon in the absence of the ribosome releasing factor
    K Ogawa
    Biochim Biophys Acta 402:288-96. 1975
    ..No such differential effect of suppressor tRNA was observed in the absence of RR factor. This suggests that the ribosome has to be released from mRNA by RR factor in order for the amber mutation to be effective...
  14. ncbi Sequence and characterization of the gcpE gene of Escherichia coli
    J Baker
    Department of Microbiology, Southern Illinois University, Carbondale 62901
    FEMS Microbiol Lett 73:175-80. 1992
    ..The probable transcription terminator of gcpE overlaps the hisS promoter. Haploid cells containing a disrupted gcpE could not be obtained, suggesting that the gcpE gene is essential...
  15. ncbi The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL)
    J C Lazzaroni
    Laboratoire de Microbiologie et Genetique Moleculaire, UMR 106, CNRS, Universite Claude Bernard Lyon I, Villeurbanne, France
    Mol Microbiol 6:735-42. 1992
    ..The carboxy-terminal part of the protein is necessary for correct interaction of PAL with the peptidoglycan layer...
  16. ncbi Corrected sequence of the mannitol (mtl) operon in Escherichia coli
    W Jiang
    Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg 24061 0308
    Mol Microbiol 4:2003-6. 1990
    ....
  17. ncbi Characterization of Lrp, and Escherichia coli regulatory protein that mediates a global response to leucine
    D A Willins
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    J Biol Chem 266:10768-74. 1991
    ..Lrp has some amino acid sequence similarity to AsnC, a protein that regulates asnA expression (Kolling, R., and Lother, H. (1985) J. Bacteriol. 164, 310-315)...
  18. pmc surA, an Escherichia coli gene essential for survival in stationary phase
    A Tormo
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    J Bacteriol 172:4339-47. 1990
    ..Cells containing suppressing mutations then grew in the same culture to 10(8) cells per ml, taking over the population. The implications of these observations to our understanding of stationary-phase mutagenesis are discussed...
  19. pmc Nucleotide sequence analysis of the purEK operon encoding 5'-phosphoribosyl-5-aminoimidazole carboxylase of Escherichia coli K-12
    A A Tiedeman
    Seattle Biomedical Research Institute, Washington 98109
    J Bacteriol 171:205-12. 1989
    ..The purEK operon is regulated by the purR gene product, and a purR regulatory-protein-binding site related to the sequences found in other pur loci was identified in the purEK operon control region...
  20. pmc DNA cleavage by restriction endonuclease PflMI is inhibited in recognition sites modified by dcm methylation
    R A Sturm
    Cold Spring Harbor Laboratory, NY 11724
    Nucleic Acids Res 17:3615. 1989
  21. pmc Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane
    J Stader
    J Bacteriol 166:244-52. 1986
    ..The results further reinforce the concept that the total flagellar motor consists of more than just the basal body...
  22. pmc Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase
    S P Salowe
    J Bacteriol 165:363-6. 1986
    ..Purification to homogeneity of the overproduced protein was accomplished by using DEAE and quaternary aminoethyl ion-exchange resins...
  23. ncbi Cloning and expression of the succinyl-CoA synthetase genes of Escherichia coli K12
    D Buck
    J Gen Microbiol 132:1753-62. 1986
    ..Further enzymological studies indicated that expression of succinyl-CoA synthetase is coordinately regulated with 2-oxoglutarate dehydrogenase...
  24. ncbi Is there a correlation between membrane phospholipid metabolism and cell division?
    G P Michel
    Ann Inst Pasteur Microbiol 136:111-8. 1985
    ..Future work will show how all these correlations function on the genetic and molecular levels...
  25. pmc Absence in Bacillus subtilis and Staphylococcus aureus of the sequence-specific deoxyribonucleic acid methylation that is conferred in Escherichia coli K-12 by the dam and dcm enzymes
    B Dreiseikelmann
    J Bacteriol 147:259-61. 1981
    ..The results showed that neither B. subtilis nor S. aureus methylates deoxyribonucleic acid at the same sites and nucleotides which are recognized and methylated by dam and dcm enzymes in E. coli K-12...
  26. ncbi Genetic analysis of the tryptophan operon regulatory region using site-directed mutagenesis
    R Kolter
    J Mol Biol 175:299-312. 1984
    ..Additional mutations support the conclusion that the last stem and loop structure in the terminated transcript, structure 3:4, is sufficient to cause transcription termination...
  27. pmc Proton-linked D-xylose transport in Escherichia coli
    V M Lam
    J Bacteriol 143:396-402. 1980
    ..Its specificity for xylose as inducer and substrate and the genetic location of a xylose-H+ transport-negative mutation near mtl showed that the xylose-H+ system is distinct from other proton-linked sugar transport systems of E. coli...
  28. pmc Molecular basis of valine resistance in Escherichia coli K-12
    R P Lawther
    Proc Natl Acad Sci U S A 78:922-5. 1981
    ....
  29. ncbi Cloning and sequencing of a previously unidentified gene that is involved in the biosynthesis of heme in Escherichia coli
    T Nakayashiki
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Gene 153:67-70. 1995
    ..H103 cells carrying a plasmid that included only hemA as an insert accumulated protoPor and coproPor, but showed no sensitivity to light, a result that suggests that it may be deficient in protoporphyrinogen oxidase activity...
  30. ncbi Nucleotide sequence of the hemG gene involved in the protoporphyrinogen oxidase activity of Escherichia coli K12
    A Sasarman
    Department of Microbiology and Immunology, Universite de Montreal, QC, Canada
    Can J Microbiol 39:1155-61. 1993
    ..The identity of the first 18 amino acids at the amino-terminal end of the protein was confirmed by microsequencing. To our knowledge, this is the first cloning of a gene involved in the protoporphyrinogen oxidase activity of E. coli...
  31. ncbi The crystal structure of succinyl-CoA synthetase from Escherichia coli at 2.5-A resolution
    W T Wolodko
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biol Chem 269:10883-90. 1994
    ..A short segment of the beta-subunit from one alpha beta-dimer is in close proximity to the CoA-binding site of the other alpha beta-dimer, providing a possible rationale for the overall tetrameric structure...
  32. ncbi An Escherichia coli protein consisting of a domain homologous to FK506-binding proteins (FKBP) and a new metal binding motif
    C Wulfing
    Protein Engineering Group, Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    J Biol Chem 269:2895-901. 1994
    ..The protein binds Ni2+ and Zn2+ tightly with 1:1 stoichiometry, Cu2+ and Co2+ with lower affinity, and Mn2+, Fe2+, Fe3+, Mg2+, and Ca2+ hardly at all...
  33. ncbi Isolation and characterization of a temperature-sensitive mutant of Salmonella typhimurium defective in prolipoprotein modification
    K Gan
    Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799
    J Biol Chem 268:16544-50. 1993
    ..These results suggest that the cloned gene encodes prolipoprotein glyceryl transferase (lgt), and in the wild-type background, this prolipoprotein modification enzyme is essential for the growth and viability of S. typhimurium...
  34. pmc Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli
    S P Cohen
    Department of Molecular Biology, Tufts University School of Medicine, Boston, Massachusetts
    J Bacteriol 175:1484-92. 1993
    ..The function of ORF72, designated marB, encoding the third putative protein in the operon, and that of other ORFs detected within the 7.8-kb fragment have not yet been determined...
  35. pmc Repression of the Escherichia coli modABCD (molybdate transport) operon by ModE
    A M Grunden
    Department of Microbiology and Cell Science, University of Florida, Gainesville 32611, USA
    J Bacteriol 178:735-44. 1996
    ..These results show that molybdate transport in E. coli is regulated by ModE, which acts as a repressor when bound to molybdate...
  36. ncbi Expression, lipoylation and structure determination of recombinant pea H-protein in Escherichia coli
    D Macherel
    Laboratoire de Physiologie Cellulaire Végétale, URA CNRS No 576, Départment de Biologie et Moléculaire Structurale, CEA Grenoble, France
    Eur J Biochem 236:27-33. 1996
    ..It is postulated that the high level of expression and lipoylation of recombinant H protein may be due to the protein retaining the structure of the original enzyme...
  37. ncbi A periplasmic protein (Skp) of Escherichia coli selectively binds a class of outer membrane proteins
    R Chen
    Max Planck Institut fur Biologie, Tubingen, Germany
    Mol Microbiol 19:1287-94. 1996
    ..The mutant was viable but possessed much-reduced concentrations of outer membrane proteins. This defect was fully restored by a plasmid-borne skp gene which may serve as a periplasmic chaperone...
  38. pmc Genetic control of the resistance to phage C1 of Escherichia coli K-12
    N A Likhacheva
    State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia
    J Bacteriol 178:5309-15. 1996
    ..According to our data the DcrA and DcrB proteins are not involved in these processes. However, the DcrA protein probably participates in some cell division steps...
  39. ncbi Mutational analysis of the ATP-binding site in HslU, the ATPase component of HslVU protease in Escherichia coli
    D H Shin
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, South Korea
    FEBS Lett 398:151-4. 1996
    ..These results suggest that the mutation in the ATP-binding site results in prevention of the binding of the adenine nucleotides to HslU and hence in impairment of both oligomerization and ATPase function of HslU...
  40. ncbi Mutagenesis of two N-terminal Thr and five Ser residues in HslV, the proteolytic component of the ATP-dependent HslVU protease
    S J Yoo
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, South Korea
    FEBS Lett 412:57-60. 1997
    ..The mutational studies also revealed that both Thr, Ser103, and Ser172, but not Ser124, are involved in the interaction of HslV with HslU and hence in the activation of HslU ATPase as well as in the HslVU complex formation...
  41. ncbi A new nos gene downstream from nosDFY is essential for dissimilatory reduction of nitrous oxide by Rhizobium (Sinorhizobium) meliloti
    Y K Chan
    Eastern Cereal and Oilseed Research Centre, Agriculture and Agri Food Canada, Central Experimental Farm, Ottawa, Ontario, Canada
    Microbiology 143:2817-24. 1997
    ..It was concluded that there are seven genes constituting the nos cluster in R. meliloti. They are organized in four complementation groups and in the same orientation, spanning a distance of about 9 kb on the nod megaplasmid...
  42. ncbi Different pathways for protein degradation by the FtsH/HflKC membrane-embedded protease complex: an implication from the interference by a mutant form of a new substrate protein, YccA
    A Kihara
    Department of Cell Biology, Institute for Virus Research, Kyoto University, Japan
    J Mol Biol 279:175-88. 1998
    ..From these results we suggest that there are at least two pathways for FtsH-dependent protein degradation, only one of which (probably for membrane proteins) is subject to the HflKC-dependent interference by the YccA11 mutant substrate...
  43. ncbi Biosynthesis of pteridines in Escherichia coli. Structural and mechanistic similarity of dihydroneopterin-triphosphate epimerase and dihydroneopterin aldolase
    C Haussmann
    Department of Organic Chemistry and Biochemistry, Technische Universitat Munchen, Lichtenbergstrasse 4, D 85747 Garching, Federal Republic of Germany
    J Biol Chem 273:17418-24. 1998
    ..The open reading frame ygiG of Hemophilus influenzae specifies a protein with the catalytic properties of an aldolase. However, the genome of H. influenzae does not specify a dihydroneopterin-triphosphate epimerase...
  44. ncbi Structure of the outer membrane protein A transmembrane domain
    A Pautsch
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Freiburg im Breisgau, Germany
    Nat Struct Biol 5:1013-7. 1998
    ..The structure constitutes a beta-barrel membrane anchor that appears to be the outer membrane equivalent of the single-chain alpha-helix anchor of the inner membrane...
  45. ncbi Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis
    A Petersohn
    Institut fur Mikrobiologie und Molekularbiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Microbiology 145:869-80. 1999
    ..Further analyses of corresponding mutants might allow a first prediction of their function within the framework of the general stress regulon...
  46. pmc Protein structure prediction by global optimization of a potential energy function
    A Liwo
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    Proc Natl Acad Sci U S A 96:5482-5. 1999
    ..These results constitute an important step toward the prediction of protein structure based solely on global optimization of a potential energy function for a given amino acid sequence...
  47. pmc 16S ribosomal RNA pseudouridine synthase RsuA of Escherichia coli: deletion, mutation of the conserved Asp102 residue, and sequence comparison among all other pseudouridine synthases
    J Conrad
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101, USA
    RNA 5:751-63. 1999
    ..Aspartate102 is found in a conserved motif present in many pseudouridine synthases. The conservation and distribution of this motif in nature was assessed...
  48. ncbi Role of Escherichia coli RpoS, LexA and H-NS global regulators in metabolism and survival under aerobic, phosphate-starvation conditions
    F Gerard
    Centre National de la Recherche Scientifique, Laboratoire de Chimie Bacterienne, Marseille, France
    Microbiology 145:1547-62. 1999
    ....
  49. pmc Crystal structure of the holliday junction DNA in complex with a single RuvA tetramer
    M Ariyoshi
    Department of Structural Biology, Biomolecular Engineering Research Institute BERI, 6 2 3 Furuedai, Suita, Osaka 565 0874, Japan
    Proc Natl Acad Sci U S A 97:8257-62. 2000
    ..The local conformation near the crossover point, where two base pairs are disrupted, suggests a possible scheme for successive base pair rearrangements, which may account for smooth Holliday junction movement without segmental unwinding...
  50. ncbi Endonuclease V of Escherichia coli prevents mutations from nitrosative deamination during nitrate/nitrite respiration
    B Weiss
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mutat Res 461:301-9. 2001
    ..The overall results indicate that Endo V functions during normal growth by helping to repair nitrosatively deaminated bases in DNA, which are by-products of anaerobic nitrate/nitrite respiration...
  51. ncbi Polar explorers: membrane proteins that determine division site placement
    L I Rothfield
    Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Cell 106:13-6. 2001
  52. ncbi tRNA-guanine transglycosylase from Escherichia coli: molecular mechanism and role of aspartate 89
    J D Kittendorf
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109 1065, USA
    Biochemistry 40:14123-33. 2001
    ..These results are most consistent with aspartate 89 acting as a nucleophile in an associative catalytic mechanism...
  53. ncbi Probing the conformational change of Escherichia coli undecaprenyl pyrophosphate synthase during catalysis using an inhibitor and tryptophan mutants
    Yi Hung Chen
    Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan
    J Biol Chem 277:7369-76. 2002
    ..FPP complex, and the other two slow phases are originated from the protein conformational change. The two slow phases coincide with the time course of FPP chain elongation from C(15) to C(55) and product release...
  54. ncbi Crystallization and preliminary X-ray crystallographic studies of a mutant of ribosome recycling factor from Escherichia coli, Arg132Gly
    Hiroaki Nakano
    Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Acta Crystallogr D Biol Crystallogr 58:124-6. 2002
    ..The crystal belongs to the monoclinic space group P2(1), with unit-cell parameters a = 46.02, b = 49.27, c = 49.37 A, beta = 110.1 degrees. The currently refined structure indicates that RRF has a tRNA-like L-shaped conformation...
  55. ncbi Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme
    C Hoang
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Cell 107:929-39. 2001
    ..It accesses its substrate uridyl residue by flipping out the nucleotide and disrupts the tertiary structure of tRNA. Structural comparisons with TruB demonstrate that all Psi synthases are descended from a common molecular ancestor...
  56. pmc An iron-binding protein, Dpr, from Streptococcus mutans prevents iron-dependent hydroxyl radical formation in vitro
    Yuji Yamamoto
    Laboratory of Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Amamiya Machi, Aoba ku, Sendai 981 8555, Japan
    J Bacteriol 184:2931-9. 2002
    ..mutans and may allow this catalase- and heme-peroxidase-deficient bacterium to grow under air by limiting the iron-catalyzed Fenton reaction...
  57. ncbi OxyR: a molecular code for redox-related signaling
    Sung Oog Kim
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cell 109:383-96. 2002
    ....
  58. pmc Structural basis for recruitment of translesion DNA polymerase Pol IV/DinB to the beta-clamp
    Karen A Bunting
    The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    EMBO J 22:5883-92. 2003
    ....
  59. ncbi Vertical transmission of a fluoroquinolone-resistant Escherichia coli within an integrated broiler operation
    Andreas Petersen
    The Royal Veterinary and Agricultural University, Department of Veterinary Pathobiology, Stigbøjlen 4, 1870 Frederiksberg C, Denmark
    Vet Microbiol 116:120-8. 2006
    ....
  60. ncbi The ATP-dependent Clp protease of Escherichia coli. Sequence of clpA and identification of a Clp-specific substrate
    S Gottesman
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 265:7886-93. 1990
    ..This fusion protein is the first Clp-specific substrate described...
  61. pmc Nucleotide sequence of the nfo gene of Escherichia coli K-12
    S M Saporito
    Department of Biological Sciences, State University of New York, Albany 12222
    J Bacteriol 170:5141-5. 1988
    ....
  62. pmc Purification and characterization of the deoR repressor of Escherichia coli
    L Mortensen
    Institute of Biological Chemistry B, University of Copenhagen, Denmark
    EMBO J 8:325-31. 1989
    ....
  63. ncbi Nucleotide sequence of the dmsABC operon encoding the anaerobic dimethylsulphoxide reductase of Escherichia coli
    P T Bilous
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Mol Microbiol 2:785-95. 1988
    ..The DMS C polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the enzyme to the membrane...
  64. ncbi The primary structure of elongation factor G from Escherichia coli. A complete amino acid sequence
    - Ovchinnikov YuA
    FEBS Lett 139:130-5. 1982
  65. ncbi Sequencing and characterization of the sdaB gene from Escherichia coli K-12
    Z Shao
    Biology Dept, Concordia University, Montreal, Quebec, Canada
    Eur J Biochem 212:777-84. 1993
    ..A mutation which established expression of the sdaB gene and synthesis of L-serine deaminase 2 in minimal medium has been demonstrated to result in a change in the ribosome-binding site of the sdaB gene...
  66. pmc Multicopy suppressors of prc mutant Escherichia coli include two HtrA (DegP) protease homologs (HhoAB), DksA, and a truncated R1pA
    S Bass
    Department of Molecular Biology, Genentech Inc, South San Francisco, California 94080 4990, USA
    J Bacteriol 178:1154-61. 1996
    ..The dksA gene was independently isolated as a multicopy suppressor of a mukB mutation, which is required for chromosomal partitioning. A third dosage-dependent prc suppressor includes a truncated rare lipoprotein A (rlpA) gene...
  67. pmc Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli
    B L Schneider
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75083 0688, USA
    J Bacteriol 180:4278-86. 1998
    ..We conclude that the AST pathway is necessary for aerobic arginine catabolism in E. coli and that at least one enzyme of this pathway contributes to ornithine catabolism...
  68. ncbi Structure of the DNA repair enzyme endonuclease IV and its DNA complex: double-nucleotide flipping at abasic sites and three-metal-ion catalysis
    D J Hosfield
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 98:397-408. 1999
    ....
  69. ncbi Inhibition of Shigella flexneri-induced transepithelial migration of polymorphonuclear leucocytes by cadaverine
    B A McCormick
    Department of Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital East, Charlestown 02129, USA
    Cell Microbiol 1:143-55. 1999
    ..coli (EPEC) to induce PMN migration. These observations not only provide insight into mechanisms of S. flexneri pathogen evolution and pathogenesis, but also suggest a potential for the use of cadaverine in the treatment of dysentery...
  70. ncbi Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozyme
    V Monchois
    Information Genetique et Structurale, UMR1889 CNRS AVENTIS and Laboratoire d Ingénierie des Systèmes Macromoléculaires, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
    J Biol Chem 276:18437-41. 2001
    ..To denote this newly assigned function, ykfE has now been registered under the new gene name Ivy (inhibitor of vertebrate lysozyme) at the E. coli genetic stock center...
  71. ncbi ATPase activity of the MsbA lipid flippase of Escherichia coli
    William T Doerrler
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:36697-705. 2002
    ..These results provide the first in vitro evidence that MsbA is a lipid-activated ATPase and that hexaacylated lipid A is an especially potent activator...
  72. pmc Involvement of enterobactin synthesis pathway in production of microcin H47
    María F Azpiroz
    Sección de Fisiología y Genética Bacterianas, Facultad de Ciencias, Montevideo 11 400, Uruguay
    Antimicrob Agents Chemother 48:1235-41. 2004
    ..The relationship found between MccH47 and catecholate siderophore production is discussed, and a model outlining MccH47 synthesis is proposed...
  73. ncbi Gene fusion during the evolution of the tryptophan operon in enterobacteriaceae
    G F Miozzari
    Nature 277:486-9. 1979
  74. ncbi Nucleotide sequence of the intercistronic region preceding the gene for RNA polymerase subunit alpha in Escherichia coli
    L E Post
    J Biol Chem 254:10604-6. 1979
    ..This short distance supports the idea that some post-transcriptional regulation determines the differential synthesis of alpha and ribosomal proteins...
  75. pmc Location of the protease II gene (ptrB) on the physical map of the Escherichia coli chromosome
    A Kanatani
    School of Pharmaceutical Sciences, Nagasaki University, Japan
    J Bacteriol 174:7881. 1992
  76. ncbi A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map
    S C Andrews
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    J Gen Microbiol 137:361-7. 1991
    ..The translation product of orf3 (205 amino acid codons) resembled the iron-sulphur protein component (DMS B subunit) of the anaerobic dimethylsulphoxide reductase complex of E. coli...
  77. ncbi In vivo and in vitro mutants of FNR the anaerobic transcriptional regulator of E. coli
    A D Sharrocks
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEBS Lett 270:119-22. 1990
    ..Four independent in vivo mutants contained identical Gly-96----Asp substitutions, which may inactivate FNR by distorting a sharp turn between beta-strands in the predicted structure...
  78. pmc SecB-independent export of Escherichia coli ribose-binding protein (RBP): some comparisons with export of maltose-binding protein (MBP) and studies with RBP-MBP hybrid proteins
    D N Collier
    Department of Microbiology and Immunology, School of Medicine, University of North Carolina, Chapel Hill 27599 7290
    J Bacteriol 172:6875-84. 1990
    ..This indicates that the export mode exhibited by a given precursor protein (cotranslational versus posttranslational) is determined by properties of both the signal peptide and the mature moiety...
  79. ncbi Periplasmic binding protein structure and function. Refined X-ray structures of the leucine/isoleucine/valine-binding protein and its complex with leucine
    J S Sack
    Howard Hughes Medical Institute, Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030
    J Mol Biol 206:171-91. 1989
    ..abstract truncated at 400 words)..
  80. pmc Cytosolic factor purified from Escherichia coli is necessary and sufficient for the export of a preprotein and is a homotetramer of SecB
    M Watanabe
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021
    Proc Natl Acad Sci U S A 86:2728-32. 1989
    ..For the posttranslational integration of another preprotein, the precursor for the lamB gene product, into inverted vesicles, the 64-kDa SecB homotetramer is also required but additional factor(s) makes integration more efficient...
  81. ncbi Structure of a complex of catabolite gene activator protein and cyclic AMP refined at 2.5 A resolution
    I T Weber
    Center for Chemical Physics, National Bureau of Standards, Gaithersburg, MD 20899
    J Mol Biol 198:311-26. 1987
    ..Each cyclic AMP molecule is in an anti conformation and has ionic and hydrogen bond interactions with both subunits...
  82. ncbi Transcriptional organization of the dnaN and recF genes of Escherichia coli K-12
    M E Armengod
    Instituto de Investigaciones Citologicas, Obra Social de la Caja de Ahorros de Valencia, Spain
    J Biol Chem 263:12109-14. 1988
    ..The uncoupling of transcription and translation of the dnaN gene (when translation is interrupted by premature nonsense codons or by other mechanisms) probably results in transcription termination at termination signals in dnaN...
  83. ncbi Sequence of thioredoxin reductase from Escherichia coli. Relationship to other flavoprotein disulfide oxidoreductases
    M Russel
    Rockefeller University, New York, New York 10021
    J Biol Chem 263:9015-9. 1988
    ....
  84. pmc Role of translation and attenuation in the control of pyrBI operon expression in Escherichia coli K-12
    K L Roland
    J Bacteriol 163:991-9. 1985
    ..This mutation greatly reduced operon expression and regulation as predicted by the attenuation control model...
  85. pmc Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon
    J Carlson
    Proc Natl Acad Sci U S A 81:4294-7. 1984
    ..The sequences reveal not only the primary structures for both subunits, but also some interesting aspects of potential regulatory sites...
  86. pmc A perfectly symmetric lac operator binds the lac repressor very tightly
    J R Sadler
    Proc Natl Acad Sci U S A 80:6785-9. 1983
    ..The natural operator is apparently "flawed" by "incorrect" base pairs in the right operator half and by an "incorrect" spacing between the operator halves with respect to maximal repressor binding...
  87. ncbi Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA
    G Lentzen
    Institut fur Molekularbiologie, Universitat Witten Herdecke, Germany
    FEBS Lett 348:233-8. 1994
    ..From equilibrium titrations with fluorescent 4.5S RNA, a dissociation constant of 0.15 microns is obtained for the RNA.protein complex. The formation of the complex is not affected by GTP binding to or hydrolysis by P48...
  88. ncbi Movie of the structural changes during a catalytic cycle of nucleoside monophosphate kinases
    C Vonrhein
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Freiburg im Breisgau, Germany
    Structure 3:483-90. 1995
    ..These are concentrated in two chain segments, or domains, of 30 and 38 residues that are involved in binding of the substrates N1TP and N2MP (nucleoside tri- and monophosphates with bases N1 and N2), respectively...
  89. pmc The cmk gene encoding cytidine monophosphate kinase is located in the rpsA operon and is required for normal replication rate in Escherichia coli
    J Fricke
    Department of Molecular Cell Biology, University of Copenhagen, Denmark
    J Bacteriol 177:517-23. 1995
    ..In addition to the physiological studies, the region upstream of cmk was sequenced, and 120 codons with strong homology to an uncharacterized protein of the speB operon were identified...
  90. pmc Two different Escherichia coli proP promoters respond to osmotic and growth phase signals
    J Mellies
    Section of Microbiology, University of California, Davis 95616
    J Bacteriol 177:144-51. 1995
    ..proP is therefore a member of the growing class of sigma S-dependent genes which respond to both stationary-phase and hyperosmolarity signals...
  91. ncbi Escherichia coli topoisomerase IV. Purification, characterization, subunit structure, and subunit interactions
    H Peng
    Program in Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York
    J Biol Chem 268:24481-90. 1993
    ..The inactivity of the truncated forms of ParC and ParE could be attributed to their failure to form the heterotetramer...
  92. pmc SpoU protein of Escherichia coli belongs to a new family of putative rRNA methylases
    E V Koonin
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894
    Nucleic Acids Res 21:5519. 1993
  93. ncbi Crystal structure of S-adenosylmethionine synthetase
    F Takusagawa
    Department of Chemistry, University of Kansas, Lawrence 66045 0046, USA
    J Biol Chem 271:136-47. 1996
    ..The essential divalent (Mg2+/Co2+) and monovalent (K+) metal ions and one of the product, Pi ions, were found in the active site from three separate structures...
  94. ncbi The redox state of the [2Fe-2S] clusters in SoxR protein regulates its activity as a transcription factor
    H Ding
    Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 271:33173-5. 1996
    ..The results suggest that one-electron oxidation and reduction of the [2Fe-2S] cluster regulate SoxR transcriptional activity...
  95. ncbi An essential role for water in an enzyme reaction mechanism: the crystal structure of the thymidylate synthase mutant E58Q
    C R Sage
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448, USA
    Biochemistry 35:16270-81. 1996
    ....
  96. pmc Host regulation of lysogenic decision in bacteriophage lambda: transmembrane modulation of FtsH (HflB), the cII degrading protease, by HflKC (HflA)
    A Kihara
    Department of Cell Biology, Institute for Virus Research, Kyoto University, Kyoto 606 01, Japan
    Proc Natl Acad Sci U S A 94:5544-9. 1997
    ..This study necessitates a revision of the prevailing view about the host control over lambda lysogenic decision...
  97. ncbi Cloning and sequence analysis of another Shiga-like toxin IIe variant gene (slt-IIera) from an Escherichia coli R107 strain isolated from rabbit
    S H Kim
    College of Veterinary Medicine, Gyeongsang National University, Chin Ju, South Korea
    Microbiol Immunol 41:805-8. 1997
    ..Recombinant SLT-IIera and SLT-IIe produced using an E. coli host-vector system showed similar cytotoxicity, suggesting that the variations in the structural gene of SLT-IIera have no significant effect on cytotoxic level...
  98. ncbi Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii
    L Zheng
    Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061 0346, USA
    J Biol Chem 273:13264-72. 1998
    ..These results show that the cysE-like gene located upstream from iscS is not essential for cell growth and that the cysE-like gene and the iscSUA-hscBA-fdx genes are contained within separate transcription units...
  99. ncbi GlnK, a PII-homologue: structure reveals ATP binding site and indicates how the T-loops may be involved in molecular recognition
    Y Xu
    Research School of Chemistry, Australian National University, GPO 414, Canberra, ACT, 2601, Australia
    J Mol Biol 282:149-65. 1998
    ..This sequence is more highly conserved than the functional T-loop, suggesting that ATP has an important role in PII-like proteins...
  100. ncbi FtsZ ring clusters in min and partition mutants: role of both the Min system and the nucleoid in regulating FtsZ ring localization
    X C Yu
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Microbiol 32:315-26. 1999
    ..A model is proposed in which both the inhibitory effect of the nucleoid and the regulation by MinCDE ensure that cells divide precisely at the midpoint...
  101. pmc Assessment of the allosteric mechanism of aspartate transcarbamoylase based on the crystalline structure of the unregulated catalytic subunit
    P T Beernink
    Department of Molecular and Cell Biology and Virus Laboratory, University of California, Berkeley, CA 94720 3206, USA
    Proc Natl Acad Sci U S A 96:5388-93. 1999
    ..Large differences in structure between the active C trimer and the holoenzyme:bisubstrate-analog complex call into question the view that this complex represents the activated R state of ATCase...

Research Grants20

  1. Novel Approaches for the Control of Microbial Pathogens
    JOSEPH F LUTKENHAUS; Fiscal Year: 2010
    ..Finally, the new program has been based on our track record in which eleven investigators supported by the previous COBRE have secured a total of 16 extramural grants. Eight of the eleven have secured 12 NIH grants. ..
  2. Chaperone Function in Iron-Sulfur Protein Folding
    LARRY VICKERY; Fiscal Year: 2008
    ..abstract_text> ..
  3. Postsynthetic Modifications of Bacterial Membrane Lipids
    John Cronan; Fiscal Year: 2006
    ..These processes will be studied by a combination of biochemical, genetic, and molecular biological approaches including tests of pathogenesis in a mouse model system ..
  4. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  5. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2010
    ..Practically, the results of our study may be useful as a basis for a novel strategy for generating a new class of antibiotics that trigger bacterial PCD, and thereby to help what has become a public health problem. ..
  6. Ler control of EPEC virulence
    JAY MELLIES; Fiscal Year: 2008
    ..Further elucidation of the molecular mechanisms of Ler action should lead to the development of chemotherapeutic agents directed against EPEC and related E. coli O157 bacteria. [unreadable] [unreadable] [unreadable] [unreadable]..
  7. SIGNAL SEQUENCE RECOGNITION AND PROTEIN TARGETING
    Robert Stroud; Fiscal Year: 2007
    ..These will instruct in the mechanism of replacing the role of RNA by protein, and therefore in the essence of the dynamic roles of SRP RNA and cpSRP43. ..
  8. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  9. ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFER
    PERRY FREY; Fiscal Year: 2006
    ..The contributions of beta- amino acids produced by lysine 2,3-aminomutase and arginine 2,3-aminomutase to the functions of antibiotics are not known. The beta-aminoacids may become significant in drug development. ..
  10. Molecular Basis for Transmembrane Conduction & Signaling
    Robert Stroud; Fiscal Year: 2006
    ..A further aim is to improve crystals of the Acetylcholine receptor as a means of three-dimensional structure determination of an archetype of gating mechanisms in an archetype of neuroreceptor superfamilies. ..
  11. THYMIDYLATE SYNTHASE
    Robert Stroud; Fiscal Year: 2001
    ..Irwin Kunts, UCSF. Some mechanistic question will be approached using NMR spectroscopy in collaboration with Dr. Thomas James, UCSF. ..
  12. 2005 Mechanisms of Membrane Transport Gordon Conference
    Robert Stroud; Fiscal Year: 2005
    ..abstract_text> ..
  13. TOPOLOGICAL REGULATION OF PROKARYOTIC CELL DIVISION
    Glenn King; Fiscal Year: 2005
    ..The protein structures will be used for the structure based design of novel antibacterial agents. ..
  14. ATP-DEPENDENT PROTEIN KINASES IN GRAM-POSITIVE BACTERIA
    Milton Saier; Fiscal Year: 2004
    ..abstract_text> ..
  15. STRUCTURE BASED DRUG DESIGN
    Robert Stroud; Fiscal Year: 2004
    ..Public domain access to the structures of therapeutic targets and technology will encourage their use by major pharmaceutical companies for the better design of therapeutics. ..
  16. E COLI MIN PROTEINS
    Lawrence Rothfield; Fiscal Year: 2003
    ..To characterize new minicell mutants that map outside of the minCDE locus and determine whether any of them code for the topological targets for MinD and MinE localization. ..
  17. Cryoprobe Upgrades for 500 & 600 MHz NMR Spectrometers
    Glenn King; Fiscal Year: 2003
    ..abstract_text> ..
  18. REGULATION BY THE ESCHERICHIA COLI SMALL RNA, DSRA
    DARREN SLEDJESKI; Fiscal Year: 2002
    ..3) To compare the effect of DsrA and H-NS on DNA structure in vivo. In vivo footprinting of multicopy plasmid genes regulated by H-NS/DsrA will be used to compare the effects of hns-mutations and overexpression of DsrA on DNA structure. ..
  19. BACTERIAL CELL DIVISION
    Lawrence Rothfield; Fiscal Year: 2002
    ..4. To determine whether the residual division sites that are present at the cell poles play a role in the generation of future division sites within the cell. ..