Staphylococcus aureus subsp. aureus MW2

Summary

Alias: Staphylococcus aureus (strain MW2), Staphylococcus aureus subsp. aureus str. MW2

Top Publications

  1. pmc Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates Harboring seg, seh, or sei genes
    Katsuhiko Omoe
    Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Ueda 3 18 8, Morioka, Iwate, Japan
    J Clin Microbiol 40:857-62. 2002
  2. pmc Mechanism of reduced vancomycin susceptibility conferred by walK mutation in community-acquired methicillin-resistant Staphylococcus aureus strain MW2
    Jinfeng Hu
    Department of Microbiology and Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, China
    Antimicrob Agents Chemother 59:1352-5. 2015
  3. pmc High precision multi-genome scale reannotation of enzyme function by EFICAz
    Adrian K Arakaki
    Center for the Study of Systems Biology, School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30318, USA
    BMC Genomics 7:315. 2006
  4. pmc De novo bacterial genome sequencing: millions of very short reads assembled on a desktop computer
    David Hernandez
    Genomic Research Laboratory, Infectious Diseases Service, Geneva University Hospitals and the University of Geneva, CH 1211 Geneva 4, Switzerland
    Genome Res 18:802-9. 2008
  5. pmc NorB, an efflux pump in Staphylococcus aureus strain MW2, contributes to bacterial fitness in abscesses
    Yanpeng Ding
    Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, 55 Fruit St, Boston, MA 02114 2696, USA
    J Bacteriol 190:7123-9. 2008
  6. pmc Simulated antibiotic exposures in an in vitro hollow-fiber infection model influence toxin gene expression and production in community-associated methicillin-resistant Staphylococcus aureus strain MW2
    Solen Pichereau
    Pharmacy Practice Division, School of Pharmacy, University of Wisconsin, Madison, Wisconsin, USA
    Antimicrob Agents Chemother 56:140-7. 2012
  7. pmc Synthetic teichoic acid conjugate vaccine against nosocomial Gram-positive bacteria
    Diana Laverde
    Division of Infectious Diseases, Department of Medicine, University Medical Center Freiburg, Freiburg, Germany EA4655 U2RM Stress Virulence, University of Caen Lower Normandy, Caen, France
    PLoS ONE 9:e110953. 2014
  8. doi Role of two-component systems in the resistance of Staphylococcus aureus to antibacterial agents
    Miki Kawada-Matsuo
    Department of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
    Virulence 2:427-30. 2011
  9. pmc Role of ArlRS in autolysis in methicillin-sensitive and methicillin-resistant Staphylococcus aureus strains
    Guido Memmi
    Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire, USA
    J Bacteriol 194:759-67. 2012
  10. pmc Elevated enterotoxin A expression and formation in Staphylococcus aureus and its association with prophage induction
    Rong Cao
    Applied Microbiology, Department of Chemistry, Faculty of Engineering, Lund University, Lund, Sweden
    Appl Environ Microbiol 78:4942-8. 2012

Research Grants

  1. Gordon Research Conference on Staphylococcal Diseases
    BRIGITTE BERGER BACHI; Fiscal Year: 2003

Patents

  1. Plants with increased tolerance and/or resistance to environmental stress and increased biomass production
  2. Plants with increased yield
  3. Process for the production of a fine chemical
  4. Process for the production of lutein
  5. Process for the production of fine chemicals
  6. Process for the control of production of fine chemicals
  7. Process for the production of a fine chemical

Scientific Experts

Detail Information

Publications79

  1. pmc Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates Harboring seg, seh, or sei genes
    Katsuhiko Omoe
    Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Ueda 3 18 8, Morioka, Iwate, Japan
    J Clin Microbiol 40:857-62. 2002
    ..aureus strains harboring seg and sei genes. These results suggest the importance of quantitative assessment of SEG and SEI production in foods in order to clarify the relationship between these new SEs and food poisoning...
  2. pmc Mechanism of reduced vancomycin susceptibility conferred by walK mutation in community-acquired methicillin-resistant Staphylococcus aureus strain MW2
    Jinfeng Hu
    Department of Microbiology and Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, China
    Antimicrob Agents Chemother 59:1352-5. 2015
    ..An electrophoretic mobility shift assay indicated that WalK (G223D)-phosphorylated WalR had a reduced capacity to bind to the atlA promoter. ..
  3. pmc High precision multi-genome scale reannotation of enzyme function by EFICAz
    Adrian K Arakaki
    Center for the Study of Systems Biology, School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30318, USA
    BMC Genomics 7:315. 2006
    ..We have performed a reannotation of 245 genomes using an updated version of EFICAz, a highly precise method for enzyme function prediction...
  4. pmc De novo bacterial genome sequencing: millions of very short reads assembled on a desktop computer
    David Hernandez
    Genomic Research Laboratory, Infectious Diseases Service, Geneva University Hospitals and the University of Geneva, CH 1211 Geneva 4, Switzerland
    Genome Res 18:802-9. 2008
    ..5- to 3.0-kb fragments. We also provide indications that the broad coverage achieved by high-throughput sequencing might allow for the detection of clonal polymorphisms in the set of DNA molecules being sequenced...
  5. pmc NorB, an efflux pump in Staphylococcus aureus strain MW2, contributes to bacterial fitness in abscesses
    Yanpeng Ding
    Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, 55 Fruit St, Boston, MA 02114 2696, USA
    J Bacteriol 190:7123-9. 2008
    ....
  6. pmc Simulated antibiotic exposures in an in vitro hollow-fiber infection model influence toxin gene expression and production in community-associated methicillin-resistant Staphylococcus aureus strain MW2
    Solen Pichereau
    Pharmacy Practice Division, School of Pharmacy, University of Wisconsin, Madison, Wisconsin, USA
    Antimicrob Agents Chemother 56:140-7. 2012
    ..SXT had minimal effects on toxin gene regulation, but it increased production and cytotoxicity of PVL toxin in the model and may enhance virulence when it is used to treat severe infections...
  7. pmc Synthetic teichoic acid conjugate vaccine against nosocomial Gram-positive bacteria
    Diana Laverde
    Division of Infectious Diseases, Department of Medicine, University Medical Center Freiburg, Freiburg, Germany EA4655 U2RM Stress Virulence, University of Caen Lower Normandy, Caen, France
    PLoS ONE 9:e110953. 2014
    ..faecalis and other Gram-positive bacteria. ..
  8. doi Role of two-component systems in the resistance of Staphylococcus aureus to antibacterial agents
    Miki Kawada-Matsuo
    Department of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
    Virulence 2:427-30. 2011
    ..Therefore, S. aureus possesses at least three TCSs that are involved in mediating its resistance to antibacterial agents...
  9. pmc Role of ArlRS in autolysis in methicillin-sensitive and methicillin-resistant Staphylococcus aureus strains
    Guido Memmi
    Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire, USA
    J Bacteriol 194:759-67. 2012
    ..Also, the autolytic phenotype in the arlRS mutant of MSSA strain Newman could be rescued by a mutation in either atl or lytM. Together, these data showed that ArlRS impacts autolysis differently in MSSA and MRSA strains...
  10. pmc Elevated enterotoxin A expression and formation in Staphylococcus aureus and its association with prophage induction
    Rong Cao
    Applied Microbiology, Department of Chemistry, Faculty of Engineering, Lund University, Lund, Sweden
    Appl Environ Microbiol 78:4942-8. 2012
    ..The high-SEA-producing group, in particular the prophage-inducible sea(1) group, may be more relevant to staphylococcal food poisoning than the low-SEA-producing group, harboring mainly sea(2)...
  11. ncbi Molecular characterization and expression of a gene encoding a Staphylococcus aureus collagen adhesin
    J M Patti
    Department of Biochemistry, University of Alabama, Birmingham 35294 0005
    J Biol Chem 267:4766-72. 1992
    ..Finally, the bacterial lysate containing the recombinant adhesin can effectively inhibit the binding of soluble collagen to cells of S. aureus...
  12. pmc A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor A
    Champion C S Deivanayagam
    Center for Biophysical Sciences and Engineering, School of Optometry, 244 CBSE, 1025 18th Street South, University of Alabama at Birmingham, Birmingham, AL 35294 0005, USA
    EMBO J 21:6660-72. 2002
    ....
  13. ncbi Staphylococcus aureus clumping factor B (ClfB) promotes adherence to human type I cytokeratin 10: implications for nasal colonization
    Louise M O'Brien
    Moyne Institute of Preventive Medicine, Department of Microbiology, Trinity College Dublin, Dublin 2, Ireland
    Cell Microbiol 4:759-70. 2002
    ..We also showed that ClfB is transcribed by S. aureus in the human nares. We propose that ClfB is a major determinant in S. aureus nasal colonization...
  14. pmc Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein
    Maria A Schumacher
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97201 3098, USA
    EMBO J 21:3546-56. 2002
    ..Such binding suggests a mechanism for Hfq function...
  15. ncbi Multiple mechanisms for the activation of human platelet aggregation by Staphylococcus aureus: roles for the clumping factors ClfA and ClfB, the serine-aspartate repeat protein SdrE and protein A
    Louise O'Brien
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
    Mol Microbiol 44:1033-44. 2002
    ..aureus. Thus, S. aureus has multiple mechanisms for stimulating platelet aggregation. Such functional redundancy suggests that this phenomenon may be important in the pathogenesis of invasive diseases such as infective endocarditis...
  16. pmc Characterization of NorR protein, a multifunctional regulator of norA expression in Staphylococcus aureus
    Que Chi Truong-Bolduc
    Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114 2696, USA
    J Bacteriol 185:3127-38. 2003
    ..Collectively, these results indicate that NorR is a multifunctional regulator, affecting cell surface properties as well as the expression of NorA and likely other multidrug resistance efflux pumps...
  17. ncbi Expression, purification, and characterization of peptidyl-tRNA hydrolase from Staphylococcus aureus
    Paul D Bonin
    Cell and Molecular Biology, Rockville, Maryland, USA
    Protein Expr Purif 24:123-30. 2002
    ..aureus Pth was determined to be 2.8 microM. A far UV circular dichroism spectrum revealed that His-tagged S. aureus Pth appears to have a structured core predominated by beta-sheet...
  18. ncbi Molecular characterization of a chromosomal locus in Staphylococcus aureus that contributes to oxidative defence and is highly induced by the cell-wall-active antibiotic oxacillin
    V K Singh
    Microbiology Group, Department of Biological Sciences, Illinois State University, Normal, IL 61790, USA
    Microbiology 147:3037-45. 2001
    ..An insertional knockout mutation in the first gene of this operon resulted in increased susceptibility of the mutant to H2O2 compared to the parent strain, but not to oxacillin...
  19. pmc Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureus
    M Bischoff
    Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
    J Bacteriol 183:5171-9. 2001
    ..The data presented here suggest that sigma(B) increases sar expression while simultaneously reducing the RNAIII level in a growth phase-dependent manner...
  20. ncbi Identification, cloning, and expression of a functional phenylalanyl-tRNA synthetase (pheRS) from Staphylococcus aureus
    J W Savopoulos
    GlaxoSmithKline Pharmaceuticals, New Frontiers Science Park North, Coldharbour Road, Harlow, Essex CM19 5AD, United Kingdom
    Protein Expr Purif 21:470-84. 2001
    ..aureus (Oxford Strain) and demonstrate that Michaelis-Menten parameters for both recombinant and native enzyme, at least for phenylalanine tRNA aminoacylation are comparable...
  21. pmc An unusual isopentenyl diphosphate isomerase found in the mevalonate pathway gene cluster from Streptomyces sp. strain CL190
    K Kaneda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 98:932-7. 2001
    ..aureus and of the different enzymatic properties of mammalian (type 1) and S. aureus (type 2) isomerases, this unusual enzyme is considered to be a suitable molecular target for the screening of antibacterial drugs specific to S. aureus...
  22. ncbi The crystal structure of staphylococcal enterotoxin H: implications for binding properties to MHC class II and TcR molecules
    M Håkansson
    Molecular Biophysics, Centre for Chemistry and Chemical Engineering, Lund University, Lund, S 221 00, Sweden
    J Mol Biol 302:527-37. 2000
    ..Since the T-cell receptor probably interacts with both domains, SEH by this rotation may modulate its binding to different TcR Vbeta-chains...
  23. ncbi Modification of autolysis by synthetic peptides derived from the presumptive binding domain of Staphylococcus aureus autolysin
    M Takano
    Pharmaceutical Development Research Laboratory, Tanabe Seiyaku Co, Ltd, Saitama, Japan
    Microbiol Immunol 44:463-72. 2000
    ..aureus autolysin activities. These peptides, especially the 10-mer peptide B9 (PGTKLYTVPW) that represents the C-terminal half of A10 and N-terminal half of A11, may be important segments for Atl to bind to the cell surface...
  24. pmc Identification and characterization of a membrane permease involved in iron-hydroxamate transport in Staphylococcus aureus
    M T Sebulsky
    Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada, N6A 5C1
    J Bacteriol 182:4394-400. 2000
    ..By using the Fur titration assay, it was shown that the Fur box sequences upstream of fhuCBG are recognized by the Escherichia coli Fur protein...
  25. ncbi Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus
    Miki Matsuo
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3 1, 7 chome, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    FEMS Microbiol Lett 222:107-13. 2003
    ..These results suggest that the murB gene is essential for growth of S. aureus and that domain 3 is important for the MurB activity...
  26. pmc Crystal structure of the SarS protein from Staphylococcus aureus
    Ronggui Li
    Integrated Department of Immunology, National Jewish Medical and Research Center, and Department of Pharmacology, Biomolecular Structure Program, School of Medicine, University of Colorado Health Science Center, Denver, Colorado 80206, USA
    J Bacteriol 185:4219-25. 2003
    ..Based on the structural differences of SarR, SarS, and MarR, we further classified these winged-helix proteins to three subfamilies, SarA, SarS, and MarR. Finally, a possible transcription regulation mechanism is proposed...
  27. ncbi Conformational movements and cooperativity upon amino acid, ATP and tRNA binding in threonyl-tRNA synthetase
    Alfredo Torres-Larios
    Laboratoire de Biologie et Genomique Structurales, IGBMC, 1 rue Laurent Fries, BP 10142, 67400 Illkirch Cedex, France
    J Mol Biol 331:201-11. 2003
    ..The structural analysis suggests that, while the small substrates can bind in any order, they must be in place before productive tRNA binding can occur...
  28. pmc MsrR, a putative cell envelope-associated element involved in Staphylococcus aureus sarA attenuation
    Jutta Rossi
    Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
    Antimicrob Agents Chemother 47:2558-64. 2003
    ..These observations suggest that MsrR is a new component involved in sarA attenuation and the regulatory network controlling virulence gene expression in S. aureus...
  29. pmc Transcriptional regulation of the Staphylococcus aureus thioredoxin and thioredoxin reductase genes in response to oxygen and disulfide stress
    Orit Uziel
    Department of Molecular Microbiology and Biotechnology, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel
    J Bacteriol 186:326-34. 2004
    ..This observation, coupled with structural differences between the bacterial and mammalian thioredoxin reductases, suggests that it may serve as a target for the development of new antimicrobials...
  30. ncbi Genetic analysis of 17 genes in Staphylococcus aureus with reduced susceptibility to vancomycin (VISA) and heteroVISA
    M Wootton
    J Antimicrob Chemother 53:406-7. 2004
  31. ncbi Expression of staphylococcal clumping factor A impedes macrophage phagocytosis
    Niklas Palmqvist
    Department of Rheumatology and Inflammation Research, Goteborg University, Guldhedsgatan 10 A, S 413 46 Goteborg, Sweden
    Microbes Infect 6:188-95. 2004
    ..Both the protection against phagocytosis and the enhanced immunostimulatory activity provided by ClfA expression are likely to contribute to the in vivo virulence of S. aureus...
  32. pmc Regulatory elements of the Staphylococcus aureus protein A (Spa) promoter
    Jinxin Gao
    Department of Diagnostic Medicine Pathobiology, College of Veterinary Medicine, Kansas State University, 1800 Denison Ave, Manhattan, KS 66506, USA
    J Bacteriol 186:3738-48. 2004
    ..Full repression required the presence of a second consensus site adjacent to the SarS binding site. Sequences directly upstream of the core promoter sequence were found to stimulate transcription...
  33. pmc Multidrug resistance in Staphylococcus aureus due to overexpression of a novel multidrug and toxin extrusion (MATE) transport protein
    Glenn W Kaatz
    The John D Dingell Department of Veteran s Affairs Medical Center, Detroit, MI 48201, USA
    Antimicrob Agents Chemother 49:1857-64. 2005
    ..MepR overexpression reversed the MDR phenotypes of both mutants by repressing mepA transcription. All three ORFs are preferentially transcribed as a single mepRAB unit, suggesting that the three genes form an operon...
  34. ncbi Structure and biosynthesis of staphyloxanthin from Staphylococcus aureus
    Alexandra Pelz
    Department of Microbial Genetics, University of Tuebingen, Germany
    J Biol Chem 280:32493-8. 2005
    ..Staphyloxanthin was identified as beta-D-glucopyranosyl 1-O-(4,4'-diaponeurosporen-4-oate)-6-O-(12-methyltetradecanoate)...
  35. pmc SarA positively controls bap-dependent biofilm formation in Staphylococcus aureus
    Maria Pilar Trotonda
    Instituto Valenciano de Investigaciones Agrarias, Carretera Náquera Moncada Km 4, 5, 46113 Moncada, Valencia, Spain
    J Bacteriol 187:5790-8. 2005
    ..Based on the previous studies of others as well as our work demonstrating the role for SarA in icaADBC and bap expression, we propose that SarA is an essential regulator controlling biofilm formation in S. aureus...
  36. pmc Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel Staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IV
    Susan Boyle-Vavra
    Department of Pediatrics, Section of Infectious Diseases, University of Chicago, IL, 60637, USA
    J Clin Microbiol 43:4719-30. 2005
    ..SCCmec V(T) is a novel SCCmec variant that is found in multiply resistant CAMRSA strains with sequence type 59 in Taipei in association with the PVL leukotoxin genes...
  37. pmc A subset of Staphylococcus aureus strains harboring staphylococcal cassette chromosome mec (SCCmec) type IV is deficient in CcrAB-mediated SCCmec excision
    Michael J Noto
    Department of Microbiology, Virginia Commonwealth University School of Medicine, Richmond, VA 23298 0565, USA
    Antimicrob Agents Chemother 50:2782-8. 2006
    ..Acquisition of this mobile element, containing a known virulence gene, appears to have stabilized the chromosomal integration of the methicillin resistance gene in these strains...
  38. pmc Direct hemin transfer from IsdA to IsdC in the iron-regulated surface determinant (Isd) heme acquisition system of Staphylococcus aureus
    Mengyao Liu
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59718, USA
    J Biol Chem 283:6668-76. 2008
    ....
  39. doi Structural characterization of the alpha-hemolysin monomer from Staphylococcus aureus
    Christian Meesters
    Institute of Molecular Biophysics, University of Mainz, Mainz, Germany
    Proteins 75:118-26. 2009
    ..This structure reveals details of the monomeric conformation of the alpha-hemolysin, for example inherent flexibility, along with definite differences in comparison to the structures used as templates...
  40. ncbi The phosphoenolpyruvate:sugar phosphotransferase system in gram-positive bacteria: properties, mechanism, and regulation
    J Reizer
    Department of Biology, University of California at San Diego, La Jolla
    Crit Rev Microbiol 15:297-338. 1988
    ....
  41. pmc A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, sequence analysis, and characterization
    T Oshida
    Rockefeller University, New York, NY 10021
    Proc Natl Acad Sci U S A 92:285-9. 1995
    ..aureus...
  42. ncbi Catalase deficiency in Staphylococcus aureus subsp. anaerobius is associated with natural loss-of-function mutations within the structural gene
    R Sanz
    Departamento Patologia Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain
    Microbiology 146:465-75. 2000
    ..aureus subsp. anaerobius, as deduced from results obtained with chimeric catalase constructs...
  43. pmc Crystal structure of the superantigen staphylococcal enterotoxin type A
    E M Schad
    Molecular Biophysics, Chemical Centre, Lund University
    EMBO J 14:3292-301. 1995
    ..Four amino acids including Ser1, His187, His225 and Asp227 were found to be involved in direct coordination of the metal ion. SEA is the first Zn2+ binding enterotoxin that has been structurally determined...
  44. ncbi Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitors
    P Staedtler
    Arbeitsgruppe Physiologie der Mikroorganismen, Abteilung Biologie, Ruhr Universitat Bochum, Germany
    Eur J Biochem 232:658-63. 1995
    ..It was shown to be identical to an authentic, synthetic sample. From this, it is evident that E375 is the active-site nucleophile of 6-phospho-galactosidase, consistent with previous findings for enzymes in this family...
  45. ncbi A homologue to the Escherichia coli alkyl hydroperoxide reductase AhpC is induced by osmotic upshock in Staphylococcus aureus
    L Armstrong-Buisseret
    Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Leicestershire, UK
    Microbiology 141:1655-61. 1995
    ..Exposure of S. aureus to varying concentrations of H202 did not result in the detectable induction of AhpC...
  46. pmc Primary sequence of the alpha-toxin gene from Staphylococcus aureus wood 46
    G S Gray
    Infect Immun 46:615-8. 1984
    ..The mature alpha-toxin protein had a molecular size of 33,000 and contained only three short regions of high hydrophobicity in addition to a number of short, weakly hydrophobic regions...
  47. ncbi A genetic system for analysis of staphylococcal nuclease
    D Shortle
    Gene 22:181-9. 1983
    ..In combination with present methods for efficient in vitro mutagenesis, this plasmid-based genetic system can be applied to the detailed genetic analysis of this small, biochemically and biophysically well-characterized enzyme...
  48. ncbi Amino acid sequence of the amphiphilic phosphocarrier protein factor IIILac of the lactose-specific phosphotransferase system of Staphylococcus
    K Stuber
    Biochemistry 24:1164-8. 1985
    ..The N-terminal part of the protein comprising approximately one-third of the chain exhibits in vitro affinity toward membrane-bound enzyme IILac...
  49. ncbi Complete amino acid sequence of staphylococcal enterotoxin A
    I Y Huang
    J Biol Chem 262:7006-13. 1987
    ..and Bergdoll, M. S. (1970) J. Biol. Chem. 245, 3518-3525), and C1 (Schmidt, J. J., and Spero, L. (1983) J. Biol. Chem. 258, 6300-6306) than that seen between enterotoxins B and C1...
  50. ncbi Comparative biochemical and molecular analysis of the Staphylococcus hyicus, Staphylococcus aureus and a hybrid lipase. Indication for a C-terminal phospholipase domain
    K Nikoleit
    Universitat Tubingen, Germany
    Eur J Biochem 228:732-8. 1995
    ..hyicus lipase. The fact that two closely related enzymes differ in the need for Ca2+ underscores the notion that it plays a structural rather than a catalytic role...
  51. ncbi Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli
    H L Shuttleworth
    Microbial Technology Laboratory, PHLS, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts, U K
    Gene 58:283-95. 1987
    ..The homology to the latter probably identifies the Fc-binding region in the immunoglobulin-binding domains of SPA. Enhanced production of SPA in Escherichia coli was achieved using the lac promoter immediately upstream from the spa gene...
  52. pmc Nucleotide sequence of the type C3 staphylococcal enterotoxin gene suggests that intergenic recombination causes antigenic variation
    J L Couch
    Department of Bacteriology, University of Wisconsin Madison 53706
    J Bacteriol 171:4507-10. 1989
    ..Sequence comparisons between the entC3, entC1, and entB genes suggest that an ancestral entC1-like gene was formed by recombination between the entC3 and entB genes...
  53. ncbi A point mutation in norA gene is responsible for quinolone resistance in Staphylococcus aureus
    Y Ohshita
    Department of Microbiology, Faculty of Medicine, Juntendo University, Tokyo, Japan
    Biochem Biophys Res Commun 172:1028-34. 1990
    ....
  54. pmc Complete nucleotide sequence of IS431mec in Staphylococcus aureus
    L Barberis-Maino
    Institute of Medical Microbiology, University of Zurich, Switzerland
    Nucleic Acids Res 18:5548. 1990
  55. pmc A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction
    P Duwat
    Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    J Bacteriol 174:5171-5. 1992
    ..This method is particularly useful for the recovery of the recA genes of gram-positive bacteria and avoids the difficulties of using a genetic complementation test for cloning...
  56. ncbi Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activity
    B Walker
    Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545
    J Biol Chem 267:10902-9. 1992
    ..The mutant polypeptide nevertheless binds tightly to erythrocytes as a monomer, strengthening the idea that oligomerization is required for cell lysis...
  57. ncbi Cleavage of human immunoglobulins by serine proteinase from Staphylococcus aureus
    L Prokesova
    Laboratory of Special Medical Microbiology and Immunology, 1st Medical Faculty, Charles University, Studnickova, Prague, Czechoslovakia
    Immunol Lett 31:259-65. 1992
    ..SP has to be considered as one of the virulence factors of S. aureus that may protect bacteria against the defence mechanisms of the host...
  58. pmc Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr
    A L Cheung
    Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, NY 10021
    Proc Natl Acad Sci U S A 89:6462-6. 1992
    ..aureus strain with a genetic background similar to strain 8325-4. This locus on the S. aureus chromosome, possibly regulatory in nature, has been designated sar for staphylococcal accessory regulator...
  59. ncbi Molecular evolution of the staphylococcal and streptococcal pyrogenic toxin gene family
    R A Van Den Bussche
    Department of Biological Sciences, University of Idaho, Moscow 83843
    Mol Phylogenet Evol 2:281-92. 1993
    ....
  60. pmc Antibacterial action of extracellular mammalian group IIA phospholipase A2 against grossly clumped Staphylococcus aureus
    M E Dominiecki
    Department of Microbiology, New York University School of Medicine, New York, New York 10016, USA
    Infect Immun 67:2299-305. 1999
    ..Thus, the extracellular mobilization of group IIA PLA2 during inflammation may provide a mechanism by which the host can control the proliferation and survival of S. aureus even after bacterial clumping...
  61. ncbi Antiseptic susceptibility and distribution of antiseptic-resistance genes in methicillin-resistant Staphylococcus aureus
    N Noguchi
    Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan
    FEMS Microbiol Lett 172:247-53. 1999
    ..Our results indicated that four or more antiseptic-resistance genes exist in methicillin-resistant S. aureus and that antiseptic-resistant methicillin-resistant S. aureus strains without qacA and smr are widely spread in Japan...
  62. pmc Identification and characterization of SirA, an iron-regulated protein from Staphylococcus aureus
    J H Heinrichs
    MedImmune, Inc, Gaithersburg, Maryland 20878, USA
    J Bacteriol 181:1436-43. 1999
    ..aureus culture medium. sir-encoded proteins may be involved in iron acquisition in vivo and therefore may be targets for antimicrobial agents...
  63. pmc Transcriptional analysis of the Staphylococcus aureus penicillin binding protein 2 gene
    M G Pinho
    Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 180:6077-81. 1998
    ..We now show that introduction of the intact pbp2 gene with its two newly identified promoters into the chromosome of the transposon mutant resulted in the full recovery of high-level methicillin resistance...
  64. ncbi Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus
    D Ni Eidhin
    Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
    Mol Microbiol 30:245-57. 1998
    ....
  65. ncbi Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family
    A Gravet
    UPRES EA 1318, Institut de Bacteriologie de la Faculte de Medecine, Université Louis Pasteur Hôpitaux Universitaires de Strasbourg, France
    FEBS Lett 436:202-8. 1998
    ..005). LukE+LukD was as effective as the Panton-Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus...
  66. ncbi Subcellular localization of the major autolysin, ATL and its processed proteins in Staphylococcus aureus
    H Komatsuzawa
    Department of Microbiology, Hiroshima University School of Dentistry, Japan
    Microbiol Immunol 41:469-79. 1997
    ..We suggest that the 138-kDa ATL undergoes processing through intermediate proteins (115- and 85-kDa proteins) to mature as the active cell cluster-dispersing enzymes 51-kDa GL and 62-kDa AM on the cell surface...
  67. ncbi Glutamine synthetase and heteroresistance in methicillin-resistant Staphylococcus aureus
    A M Stranden
    Institute of Medical Microbiology, University of Zurich, Switzerland
    Microb Drug Resist 2:201-7. 1996
    ..This suggests that S. aureus can adopt multiple ways to achieve high methicillin resistance...
  68. pmc Localized perforation of the cell wall by a major autolysin: atl gene products and the onset of penicillin-induced lysis of Staphylococcus aureus
    M Sugai
    Department of Microbiology, Hiroshima University School of Dentistry, Japan
    J Bacteriol 179:2958-62. 1997
    ....
  69. pmc Staphylococcus aureus penicillin-binding protein 4 and intrinsic beta-lactam resistance
    U U Henze
    Institute of Medical Microbiology, University of Zurich, Switzerland
    Antimicrob Agents Chemother 39:2415-22. 1995
    ..The abcA-pbp4-tagD gene cluster was located in the SmaI-D fragment in the S. aureus 8325 chromosome in close proximity to the RNA polymerase gene rpoB...
  70. pmc Histidine residues near the N terminus of staphylococcal alpha-toxin as reporters of regions that are critical for oligomerization and pore formation
    R Jursch
    Institute of Medical Microbiology, University of Mainz, Germany
    Infect Immun 62:2249-56. 1994
    ..We interpret these results to indicate that the N-terminal domain of alpha-toxin in the region of H-35 and H-48 is involved in protomer-protomer interactions that underlie the hexamerization and pore-forming process...
  71. ncbi A genetic and molecular characterization of the recA gene from Staphylococcus aureus
    K W Bayles
    Department of Biological Sciences, University of Maryland, Baltimore 21228
    Gene 147:13-20. 1994
    ..Furthermore, genetic and physical mapping of S. aureus recA placed it in the same region as the uvs-568 mutation. These data strongly suggest that these mutations represent different alleles of the same recA gene...
  72. ncbi Identification of a chromosomally encoded ABC-transport system with which the staphylococcal erythromycin exporter MsrA may interact
    J I Ross
    Department of Microbiology, University of Leeds, UK
    Gene 153:93-8. 1995
    ..There was no further sequence similarity outside these conserved regions. These results indicate that the chromosomes of S. hominis and S. aureus contain sequences encoding a potential TM protein with which MsrA might interact...
  73. pmc Secretion of staphylococcal nuclease by Bacillus subtilis
    S Kovacevic
    J Bacteriol 162:521-8. 1985
    ..These findings indicate the prospective use of nuclease as a model system for studying secretion of heterologous proteins in B. subtilis...
  74. pmc Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains
    Xiao Xue Ma
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Antimicrob Agents Chemother 46:1147-52. 2002
    ..Consistent with the strains' susceptibilities to various non-beta-lactam antibiotics, the type IV SCCmec was devoid of any antibiotic resistance genes other than the mecA gene...
  75. pmc Identification and characterization of the PutP proline permease that contributes to in vivo survival of Staphylococcus aureus in animal models
    W R Schwan
    PathoGenesis Corporation, Seattle, Washington 98119, USA
    Infect Immun 66:567-72. 1998
    ..aureus high-affinity proline permease suggests that proline scavenging by the bacteria is important for in vivo growth and proliferation and that analogs of proline may serve as potential antistaphylococcal therapeutic agents...
  76. ncbi Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore
    L Song
    Department of Biochemistry, University of Chicago, 920 East 58 Street, Chicago, IL 60637, USA
    Science 274:1859-66. 1996
    ....
  77. pmc Novel non-mecA-containing staphylococcal chromosomal cassette composite island containing pbp4 and tagF genes in a commensal staphylococcal species: a possible reservoir for antibiotic resistance islands in Staphylococcus aureus
    Kanokporn Mongkolrattanothai
    Section of Pediatric Infectious Diseases, Department of Pediatrics, University of Chicago, Chicago, Illinois 60637, USA
    Antimicrob Agents Chemother 48:1823-36. 2004
    ..In view of the fact that SCC-CI was found in a commensal species, it may represent a reservoir for sequences involved in genetic shuffling between staphylococci and may contribute to the diversity found in SCC elements...
  78. ncbi Chromosome-determined zinc-responsible operon czr in Staphylococcus aureus strain 912
    M Kuroda
    Department of Microbiology, Institute of Basic Medical Sciences, and College of Medical Technology, University of Tsukuba, Ibaraki, Japan
    Microbiol Immunol 43:115-25. 1999
    ..25 mg per g dry weight. The findings indicated that the novel operon czrAB should play a role in the transportation of zinc across the cell membrane to maintain the proper intracellular concentration...
  79. pmc Staphylococcus aureus chromosomal mutation plaC1 amplifies plasmid pT181 by depressing synthesis of its negative-effector countertranscripts
    S Iordanescu
    Department of Plasmid Biology, Public Health Research Institute, New York, New York 10016
    J Bacteriol 171:4831-5. 1989
    ..In turn, the reduction in countertranscript synthesis leads to an increase in the production of the initiator protein RepC, which is limiting for plasmid replication, and a higher plasmid copy number...

Research Grants1

  1. Gordon Research Conference on Staphylococcal Diseases
    BRIGITTE BERGER BACHI; Fiscal Year: 2003
    ..S. This will afford a unique and rich opportunity for fellowship and scientific exchange between young and established investigators in the field. ..

Patents7

  1. Plants with increased tolerance and/or resistance to environmental stress and increased biomass production
    Patent Number: WO2008142034-A2; Date:2008-11-27
  2. Plants with increased yield
    Patent Number: WO2009037279-A1; Date:2009-03-26
  3. Process for the production of a fine chemical
    Patent Number: EP2090662-A2; Date:2009-08-19
  4. Process for the production of lutein
    Patent Number: EP2096177-A2; Date:2009-09-02
  5. Process for the production of fine chemicals
    Patent Number: EP2194140-A2; Date:2010-06-09
  6. Process for the control of production of fine chemicals
    Patent Number: EP2199304-A1; Date:2010-06-23
  7. Process for the production of a fine chemical
    Patent Number: WO2008034648-A1; Date:2008-03-27