Genomes and Genes
Staphylococcus aureus subsp. aureus COL
Alias: Staphylococcus aureus COL
- Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureusH L Peng
Public Health Research Institute, New York, New York 10016
J Bacteriol 170:4365-72. 1988....
- Identification and characterization of SarH1, a new global regulator of virulence gene expression in Staphylococcus aureusK Tegmark
Microbiology and Tumorbiology Center MTC, Box 280, Karolinska Institutet, S 17177 Stockholm, Sweden
Mol Microbiol 37:398-409. 2000..In conclusion, we show that both the agr-dependent suppression of protein A production and the sarA-dependent stimulation of alpha-toxin production is mediated via a new regulator, SarH1, which belongs to a family of Sar homologues...
- Transcriptional induction of the penicillin-binding protein 2 gene in Staphylococcus aureus by cell wall-active antibiotics oxacillin and vancomycinSusan Boyle-Vavra
Department of Pediatrics, The University of Chicago, Illinois 60637, USA
Antimicrob Agents Chemother 47:1028-36. 2003..These data support the idea that pbpB expression is modulated by a trans-acting factor in response to the presence of the cell wall-active antibiotics vancomycin and oxacillin...
- Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with l-lysineA Peschel
Microbial Genetics, University of Tuebingen, 72076 Tuebingen, Germany
J Exp Med 193:1067-76. 2001..MprF thus constitutes a novel virulence factor, which may be of general relevance for bacterial pathogens and represents a new target for attacking multidrug resistant bacteria...
- Nucleotide sequence of the Staphylococcus aureus signal peptidase II (lsp) geneX J Zhao
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, MD 20899 4799
FEBS Lett 299:80-4. 1992..Unlike the lsp genes from the Gram-negative bacteria, the lsp gene in S. aureus is not flanked by x-ileS and orf149-orf316 as found in E. coli, Ent. aerogenes, and P. fluorescens...
- Mgr, a novel global regulator in Staphylococcus aureusThanh T Luong
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
J Bacteriol 185:3703-10. 2003..Northern slot blot analyses suggested that mgr affected CP8, alpha-toxin, nuclease, and protein A at the transcriptional level...
- DNA microarray-based identification of genes associated with glycopeptide resistance in Staphylococcus aureusLongzhu Cui
Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
Antimicrob Agents Chemother 49:3404-13. 2005..We propose that the genes which raise glycopeptide resistance in S. aureus function toward altering the cell wall metabolic pathway...
- Three-dimensional structure of the complex between a T cell receptor beta chain and the superantigen staphylococcal enterotoxin BH Li
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville 20850, USA
Immunity 9:807-16. 1998..This variability can account for the preferential expression of certain V alpha regions among T cells reactive with SEB...
- Intranasal exposure to bacterial superantigens induces airway inflammation in HLA class II transgenic miceGovindarajan Rajagopalan
Department of Immunology, Mayo Clinic College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
Infect Immun 74:1284-96. 2006..In conclusion, repeated chronic intranasal exposure to bacterial superantigens causes airway inflammation and systemic immune activation...
- A novel loop domain in superantigens extends their T cell receptor recognition siteSebastian Günther
Boston Biomedical Research Institute, Watertown, MA 02472, USA
J Mol Biol 371:210-21. 2007..These interactions are absolutely required for TCR binding and T cell activation by SEK, and dictate the TCR Vbeta domain specificity of SEK and other group V SAGs...
- Identification of a novel two-component regulatory system that acts in global regulation of virulence factors of Staphylococcus aureusJ M Yarwood
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
J Bacteriol 183:1113-23. 2001..Furthermore, srrAB may provide a mechanistic link between respiratory metabolism, environmental signals, and regulation of virulence factors in S. aureus...
- Multiple mechanisms for the activation of human platelet aggregation by Staphylococcus aureus: roles for the clumping factors ClfA and ClfB, the serine-aspartate repeat protein SdrE and protein ALouise O'Brien
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
Mol Microbiol 44:1033-44. 2002..aureus. Thus, S. aureus has multiple mechanisms for stimulating platelet aggregation. Such functional redundancy suggests that this phenomenon may be important in the pathogenesis of invasive diseases such as infective endocarditis...
- SarT, a repressor of alpha-hemolysin in Staphylococcus aureusK A Schmidt
Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 13755, USA
Infect Immun 69:4749-58. 2001..Collectively, these results indicated that the sarA locus, contrary to the regulatory action of agr, induced alpha-hemolysin production by repressing sarT, a repressor of hla transcription...
- Identification and nucleotide sequence of the delta-lysin gene, hld, adjacent to the accessory gene regulator (agr) of Staphylococcus aureusL Janzon
Department of Bacteriology, Karolinska Institutet, Stockholm, Sweden
Mol Gen Genet 219:480-5. 1989..Determination of hld mRNA half-life in different growth phases indicated that this regulation is at the level of transcription...
- The agr P2 operon: an autocatalytic sensory transduction system in Staphylococcus aureusR P Novick
Public Health Research Institute of the City of New York, Inc, NY 10016, USA
Mol Gen Genet 248:446-58. 1995..Since transcription of P2 requires P2 operon products, the P2 operon is autocatalytic, and is thus admirably suited to the need for rapid production of exoproteins at a time when overall growth is coming to a halt...
- Role of VraSR in antibiotic resistance and antibiotic-induced stress response in Staphylococcus aureusS Gardete
Molecular Genetics Laboratory, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Oeiras, Portugal
Antimicrob Agents Chemother 50:3424-34. 2006..One of the sites in cell wall synthesis "sensed" by the VraSR system appears to be a step catalyzed by PBP 2...
- Different bacterial gene expression patterns and attenuated host immune responses are associated with the evolution of low-level vancomycin resistance during persistent methicillin-resistant Staphylococcus aureus bacteraemiaBenjamin P Howden
Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Clayton, Victoria, Australia
BMC Microbiol 8:39. 2008..Based on the transcriptome results multiple genes were sequenced and innate immune system stimulation was assessed in the VSSA and hVISA/VISA pairs...
- Identification, cloning, and initial characterization of rot, a locus encoding a regulator of virulence factor expression in Staphylococcus aureusP J McNamara
Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
J Bacteriol 182:3197-203. 2000..We named this ORF rot (for repressor of toxins) (GenBank accession no. AF189239) because of the activity associated with rot::Tn917 mutant strains...
- Functional analysis of luxS in Staphylococcus aureus reveals a role in metabolism but not quorum sensingNeil Doherty
Institute of Infections, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, Nottingham NG7 2RD, United Kingdom
J Bacteriol 188:2885-97. 2006..aureus RN6390B during intracellular growth in epithelial cells: the wild type and a luxS mutant showed very similar growth patterns after their internalization by MAC-T cells...
- Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureusE W Brunskill
Program in Molecular and Cell Biology, University of Maryland, Baltimore County 21228, USA
J Bacteriol 178:611-8. 1996..These data suggest that the lytS and lytR gene products control the rate of autolysis in S. aureus by affecting the intrinsic murein hydrolase activity associated with the cell...
- The two-component system ArlS-ArlR is a regulator of virulence gene expression in Staphylococcus aureusB Fournier
Unité de Biochimie Microbienne, URA 2172 du Centre National de la Recherche Scientifique, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
Mol Microbiol 41:247-61. 2001..Finally, arl was not autoregulated, but its expression was stimulated by agr and sarA. These results suggest that the Arl system interacts with both agr and sarA regulatory loci to modulate the virulence regulation network...
- Sigma-B, a putative operon encoding alternate sigma factor of Staphylococcus aureus RNA polymerase: molecular cloning and DNA sequencingS Wu
The Rockefeller University, New York, New York 10021, USA
J Bacteriol 178:6036-42. 1996..subtilis sigB operon. A Tn551 insertional mutant, RUSA168 (insert in orf256 of the staphylococcal sigma-B operon), showed drastic reduction in methicillin resistance (decrease in MIC from 1,600 microg ml-1 to 12 to 25 microg ml-1off..
- Cytoplasmic control of premature activation of a secreted protease zymogen: deletion of staphostatin B (SspC) in Staphylococcus aureus 8325-4 yields a profound pleiotropic phenotypeLindsey N Shaw
Department of Biochemistry and Molecular Biology, University of Georgia, Life Sciences Bldg, Athens, GA 30602, USA
J Bacteriol 187:1751-62. 2005..Seemingly, some of these proteins may play a role in protein secretion; hence, their proteolytic inactivation by SspB has pleiotropic effects on the SspC-deficient mutant...
- Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agrA L Cheung
Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, NY 10021
Proc Natl Acad Sci U S A 89:6462-6. 1992..aureus strain with a genetic background similar to strain 8325-4. This locus on the S. aureus chromosome, possibly regulatory in nature, has been designated sar for staphylococcal accessory regulator...
- Comparison of simple sequence repeats in Staphylococcus strains using in-silico approachSunil Thorat
Institute of Bioresources and Sustainable Development, Imphal 795001, Manipur, India
Bioinformation 8:1182-6. 2012..a specific group of Simple Sequence Repeats (SSRs), in genome sequences of six Staphylococcus strains (Staphylococcus aureus COL, S.aureus MRSA252, S.aureus MSSA476, S.aureus Mu50, S.aureus MW2, S...
- Metabolic footprint analysis uncovers strain specific overflow metabolism and D-isoleucine production of Staphylococcus aureus COL and HG001Kirsten Dörries
Institute of Biochemistry, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
PLoS ONE 8:e81500. 2013..Altogether these time-resolved footprint analyses give first metabolic insights into staphylococcal growth behaviour in a culture medium used for infection related studies. ..
- Four new tetramic acid and one new furanone derivatives from the plant endophytic fungus Neopestalotiopsis spShasha Zhao
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China University of Chinese Academy of Sciences, Beijing 100049, PR China
Fitoterapia 103:106-12. 2015..Compound 2 showed modest antimicrobial activity against the Gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus col, and Streptococcus pneumoniae.
- Structure of an amidohydrolase, SACOL0085, from methicillin-resistant Staphylococcus aureus COLTavarekere S Girish
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India
Acta Crystallogr Sect F Struct Biol Cryst Commun 69:103-8. 2013..A conserved cysteine residue at the active site distinguishes M20D peptidases from other M20 family members. This cysteine, Cys103, serves as bidentate ligand coordinating both Mn(2+) ions in SACOL0085...
- Comprehensive characterization of methicillin-resistant Staphylococcus aureus subsp. aureus COL secretome by two-dimensional liquid chromatography and mass spectrometryShobha Ravipaty
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA
Mol Cell Proteomics 9:1898-919. 2010..protein and peptide mass spectrometry is used to characterize proteins secreted by methicillin-resistant Staphylococcus aureus COL. Protein identifications were accomplished via off-line protein fractionation followed by digestion and ..
- Oxidative stress triggers thiol oxidation in the glyceraldehyde-3-phosphate dehydrogenase of Staphylococcus aureusHarald Weber
Institut fur Mikrobiologie, Universitat Greifswald, Jahnstrasse 15, 17487 Greifswald, Germany
Mol Microbiol 52:133-40. 2004..time-of-flight mass spectrometry (MALDI-TOF MS) was used to analyse the oxidative stress response in Staphylococcus aureus COL. Exponentially growing cells were supplemented with 100 mM H2O2 leading to a growth arrest lasting 30 ..
- Sesquiterpenes and cyclopeptides from the endophytic fungus Trichoderma asperellum SAMUELS, LIECKF. & NIRENBERGGang Ding
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, P R China
Chem Biodivers 9:1205-12. 2012..2025) with IC₅₀ values of 7.30 and 5.24 μM, and against Staphylococcus aureus COL (CGMCC 1.2465) with IC₅₀ values of 19.02 and 14.00 μM, respectively.
- Protecs, a comprehensive and powerful storage and analysis system for OMICS data, applied for profiling the anaerobiosis response of Staphylococcus aureus COLStephan Fuchs
Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
Proteomics 10:2982-3000. 2010..Interestingly, especially genes with a high codon adaptation index highly overlap with anaerobically induced genes...
- Proteolysis during long-term glucose starvation in Staphylococcus aureus COLStephan Michalik
Institute of Microbiology, Ernst Moritz Arndt University, D 17487 Greifswald, Germany
Proteomics 9:4468-77. 2009..and 2-D PAGE revealed that protein degradation appears to play a crucial role for the cell physiology of Staphylococcus aureus COL during extended periods of glucose starvation...
- A protocol for the investigation of the intracellular Staphylococcus aureus metabolomeHanna Meyer
Department of Pharmaceutical Biology, Friedrich Ludwig Jahn Street 15, 17489 Greifswald, Ernst Moritz Arndt University of Greifswald, Germany
Anal Biochem 401:250-9. 2010..Therefore, a protocol to elucidate the metabolome of the gram-positive pathogen, Staphylococcus aureus COL strain, grown in a chemically defined medium is introduced here...
- Haemin represses the haemolytic activity of Staphylococcus aureus in an Sae-dependent mannerJulia Schmitt
Institute of Medical Microbiology and Hygiene, University of Saarland Hospital, Homburg Saar, Germany
Microbiology 158:2619-31. 2012..aureus modulates its haemolysin production in response to iron and haem availability in an Sae-dependent manner...
- Role of ArlRS in autolysis in methicillin-sensitive and methicillin-resistant Staphylococcus aureus strainsGuido Memmi
Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire, USA
J Bacteriol 194:759-67. 2012..Also, the autolytic phenotype in the arlRS mutant of MSSA strain Newman could be rescued by a mutation in either atl or lytM. Together, these data showed that ArlRS impacts autolysis differently in MSSA and MRSA strains...
- A homologue to the Escherichia coli alkyl hydroperoxide reductase AhpC is induced by osmotic upshock in Staphylococcus aureusL Armstrong-Buisseret
Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Leicestershire, UK
Microbiology 141:1655-61. 1995..Exposure of S. aureus to varying concentrations of H202 did not result in the detectable induction of AhpC...
- Overproduction of a 37-kilodalton cytoplasmic protein homologous to NAD+-linked D-lactate dehydrogenase associated with vancomycin resistance in Staphylococcus aureusW M Milewski
Department of Pediatrics, University of Chicago, Illinois 60637, USA
Antimicrob Agents Chemother 40:166-72. 1996..The increased D-lactate dehydrogenase activity in strain 523k and the structural similarities among Ddh, D-lactate dehydrogenase, and VanH suggest that overproduction of Ddh might play a role in vancomycin resistance in this strain...
- Histidine residues near the N terminus of staphylococcal alpha-toxin as reporters of regions that are critical for oligomerization and pore formationR Jursch
Institute of Medical Microbiology, University of Mainz, Germany
Infect Immun 62:2249-56. 1994..We interpret these results to indicate that the N-terminal domain of alpha-toxin in the region of H-35 and H-48 is involved in protomer-protomer interactions that underlie the hexamerization and pore-forming process...
- Crystal structure of the SarS protein from Staphylococcus aureusRonggui Li
Integrated Department of Immunology, National Jewish Medical and Research Center, and Department of Pharmacology, Biomolecular Structure Program, School of Medicine, University of Colorado Health Science Center, Denver, Colorado 80206, USA
J Bacteriol 185:4219-25. 2003..Based on the structural differences of SarR, SarS, and MarR, we further classified these winged-helix proteins to three subfamilies, SarA, SarS, and MarR. Finally, a possible transcription regulation mechanism is proposed...
- MsrR, a putative cell envelope-associated element involved in Staphylococcus aureus sarA attenuationJutta Rossi
Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
Antimicrob Agents Chemother 47:2558-64. 2003..These observations suggest that MsrR is a new component involved in sarA attenuation and the regulatory network controlling virulence gene expression in S. aureus...
- Expression of staphylococcal clumping factor A impedes macrophage phagocytosisNiklas Palmqvist
Department of Rheumatology and Inflammation Research, Goteborg University, Guldhedsgatan 10 A, S 413 46 Goteborg, Sweden
Microbes Infect 6:188-95. 2004..Both the protection against phagocytosis and the enhanced immunostimulatory activity provided by ClfA expression are likely to contribute to the in vivo virulence of S. aureus...
- Identification of temperature-sensitive dnaD mutants of Staphylococcus aureus that are defective in chromosomal DNA replicationY Li
Laboratory of Developmental Biochemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3 1, 7 chome, Hongo, Bunkyo ku, 113 0033 Tokyo, Japan
Mol Genet Genomics 271:447-57. 2004..Moreover, both mutants were sensitive to mitomycin C, a drug that induces DNA damage, suggesting that the DnaD protein is also involved in DNA repair...
- Cloning and primary structure of Staphylococcus aureus DNA topoisomerase IV: a primary target of fluoroquinolonesL Ferrero
Departement des Biotechnologies, Rhone Poulenc Rorer S A, Centre de Recherche de Vitry Alfortville, Vitry sur Seine, France
Mol Microbiol 13:641-53. 1994..coli GyrA, is substituted to Phe or Tyr in both high- and low-quinolone-resistant isolates. We propose that DNA topoisomerase IV is a primary target of fluoroquinolones in S. aureus...
- Structure and biosynthesis of staphyloxanthin from Staphylococcus aureusAlexandra Pelz
Department of Microbial Genetics, University of Tuebingen, Germany
J Biol Chem 280:32493-8. 2005..Staphyloxanthin was identified as beta-D-glucopyranosyl 1-O-(4,4'-diaponeurosporen-4-oate)-6-O-(12-methyltetradecanoate)...
- Identification of a chromosomally encoded ABC-transport system with which the staphylococcal erythromycin exporter MsrA may interactJ I Ross
Department of Microbiology, University of Leeds, UK
Gene 153:93-8. 1995..There was no further sequence similarity outside these conserved regions. These results indicate that the chromosomes of S. hominis and S. aureus contain sequences encoding a potential TM protein with which MsrA might interact...
- SarA positively controls bap-dependent biofilm formation in Staphylococcus aureusMaria Pilar Trotonda
Instituto Valenciano de Investigaciones Agrarias, Carretera Náquera Moncada Km 4, 5, 46113 Moncada, Valencia, Spain
J Bacteriol 187:5790-8. 2005..Based on the previous studies of others as well as our work demonstrating the role for SarA in icaADBC and bap expression, we propose that SarA is an essential regulator controlling biofilm formation in S. aureus...
- A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, sequence analysis, and characterizationT Oshida
Rockefeller University, New York, NY 10021
Proc Natl Acad Sci U S A 92:285-9. 1995..aureus...
- Comparative biochemical and molecular analysis of the Staphylococcus hyicus, Staphylococcus aureus and a hybrid lipase. Indication for a C-terminal phospholipase domainK Nikoleit
Universitat Tubingen, Germany
Eur J Biochem 228:732-8. 1995..hyicus lipase. The fact that two closely related enzymes differ in the need for Ca2+ underscores the notion that it plays a structural rather than a catalytic role...
- Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitorsP Staedtler
Arbeitsgruppe Physiologie der Mikroorganismen, Abteilung Biologie, Ruhr Universitat Bochum, Germany
Eur J Biochem 232:658-63. 1995..It was shown to be identical to an authentic, synthetic sample. From this, it is evident that E375 is the active-site nucleophile of 6-phospho-galactosidase, consistent with previous findings for enzymes in this family...
- Conformational movements and cooperativity upon amino acid, ATP and tRNA binding in threonyl-tRNA synthetaseAlfredo Torres-Larios
Laboratoire de Biologie et Genomique Structurales, IGBMC, 1 rue Laurent Fries, BP 10142, 67400 Illkirch Cedex, France
J Mol Biol 331:201-11. 2003..The structural analysis suggests that, while the small substrates can bind in any order, they must be in place before productive tRNA binding can occur...
- Analysis of gyrA and grlA mutations in stepwise-selected ciprofloxacin-resistant mutants of Staphylococcus aureusL Ferrero
Departement des Biotechnologies, Rhone Poulenc Rorer S A, Vitry sur Seine, France
Antimicrob Agents Chemother 39:1554-8. 1995..Ferrero, B. Cameron, B. Manse, D. Lagneaux, J. Crouzet, A. Famechon, and F. Blanche, Mol. Microbiol. 13:641-653, 1994) that DNA topoisomerase IV is a primary target of fluoroquinolones in S. aureus...
- The N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly in Bacillus subtilis and Staphylococcus aureusMichael A D'Elia
Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
J Bacteriol 191:4030-4. 2009....
- Primary sequence of the alpha-toxin gene from Staphylococcus aureus wood 46G S Gray
Infect Immun 46:615-8. 1984..The mature alpha-toxin protein had a molecular size of 33,000 and contained only three short regions of high hydrophobicity in addition to a number of short, weakly hydrophobic regions...
- A genetic system for analysis of staphylococcal nucleaseD Shortle
Gene 22:181-9. 1983..In combination with present methods for efficient in vitro mutagenesis, this plasmid-based genetic system can be applied to the detailed genetic analysis of this small, biochemically and biophysically well-characterized enzyme...
- Amino acid sequence of the amphiphilic phosphocarrier protein factor IIILac of the lactose-specific phosphotransferase system of StaphylococcusK Stuber
Biochemistry 24:1164-8. 1985..The N-terminal part of the protein comprising approximately one-third of the chain exhibits in vitro affinity toward membrane-bound enzyme IILac...
- The phosphoenolpyruvate:sugar phosphotransferase system in gram-positive bacteria: properties, mechanism, and regulationJ Reizer
Department of Biology, University of California at San Diego, La Jolla
Crit Rev Microbiol 15:297-338. 1988....
- Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coliH L Shuttleworth
Microbial Technology Laboratory, PHLS, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts, U K
Gene 58:283-95. 1987..The homology to the latter probably identifies the Fc-binding region in the immunoglobulin-binding domains of SPA. Enhanced production of SPA in Escherichia coli was achieved using the lac promoter immediately upstream from the spa gene...
- The coagulase of Staphylococcus aureus 8325-4. Sequence analysis and virulence of site-specific coagulase-deficient mutantsP Phonimdaeng
Department of Microbiology, Moyne Institute, Trinity College, Dublin, Ireland
Mol Microbiol 4:393-404. 1990..This contradicts findings of several groups using Coa- mutants generated by chemical mutagenesis and suggests that the earlier results were obtained with strains that had suffered additional mutations in virulence-related genes...
- Complete nucleotide sequence of IS431mec in Staphylococcus aureusL Barberis-Maino
Institute of Medical Microbiology, University of Zurich, Switzerland
Nucleic Acids Res 18:5548. 1990
- Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activityB Walker
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545
J Biol Chem 267:10902-9. 1992..The mutant polypeptide nevertheless binds tightly to erythrocytes as a monomer, strengthening the idea that oligomerization is required for cell lysis...
- An unusual isopentenyl diphosphate isomerase found in the mevalonate pathway gene cluster from Streptomyces sp. strain CL190K Kaneda
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
Proc Natl Acad Sci U S A 98:932-7. 2001..aureus and of the different enzymatic properties of mammalian (type 1) and S. aureus (type 2) isomerases, this unusual enzyme is considered to be a suitable molecular target for the screening of antibacterial drugs specific to S. aureus...
- Identification and characterization of SirA, an iron-regulated protein from Staphylococcus aureusJ H Heinrichs
MedImmune, Inc, Gaithersburg, Maryland 20878, USA
J Bacteriol 181:1436-43. 1999..aureus culture medium. sir-encoded proteins may be involved in iron acquisition in vivo and therefore may be targets for antimicrobial agents...
- Antiseptic susceptibility and distribution of antiseptic-resistance genes in methicillin-resistant Staphylococcus aureusN Noguchi
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan
FEMS Microbiol Lett 172:247-53. 1999..Our results indicated that four or more antiseptic-resistance genes exist in methicillin-resistant S. aureus and that antiseptic-resistant methicillin-resistant S. aureus strains without qacA and smr are widely spread in Japan...
- Antibacterial action of extracellular mammalian group IIA phospholipase A2 against grossly clumped Staphylococcus aureusM E Dominiecki
Department of Microbiology, New York University School of Medicine, New York, New York 10016, USA
Infect Immun 67:2299-305. 1999..Thus, the extracellular mobilization of group IIA PLA2 during inflammation may provide a mechanism by which the host can control the proliferation and survival of S. aureus even after bacterial clumping...
- Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wallS K Mazmanian
Department of Microbiology and Immunology, UCLA School of Medicine, University of California, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
Science 285:760-3. 1999..The protein specified by srtA, sortase, may be a useful target for the development of new antimicrobial drugs...
- Topological analysis of the MraY protein catalysing the first membrane step of peptidoglycan synthesisA Bouhss
Biochimie Structurale et Cellulaire, CNRS, Batiment 430, Universite Paris Sud, F 91405, Orsay, France
Mol Microbiol 34:576-85. 1999..It was noteworthy that S. aureus MraY was functional in E. coli. An increased MraY transferase activity was observed only with the untruncated hybrids from both organisms...
- Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillinH de Lencastre
Laboratory of Microbiology, The Rockefeller University, New York, NY 10021, USA
Microb Drug Resist 5:163-75. 1999..Sequencing upstream and downstream allowed the identification of a number of additional open reading frames, some of which may also include functions relevant for the expression of antibiotic resistance...
- Autophosphorylation of phosphoglucosamine mutase from Escherichia coliL Jolly
Laboratoire des Enveloppes Bactériennes et Antibiotiques, Centre National de la Recherche Scientifique, Universite Paris Sud, 91405 Orsay, France
J Bacteriol 182:1280-5. 2000..coli GlmM...
- Molecular analysis of the accessory gene regulator (agr) locus and balance of virulence factor expression in epidemic methicillin-resistant Staphylococcus aureusH Papakyriacou
Department of Microbiology, Sunnybrook and Women s College Health Science Centre, University of Toronto, Toronto, Ontario, Canada
J Infect Dis 181:990-1000. 2000..Thus, the balance of virulence factor expression in CMRSA-1 and CMRSA-3 favors the colonization phase of infection, and CMRSA-1 possesses unique genotypic and phenotypic traits...
- Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureusD Ni Eidhin
Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
Mol Microbiol 30:245-57. 1998....
- Identification and characterization of a membrane permease involved in iron-hydroxamate transport in Staphylococcus aureusM T Sebulsky
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada, N6A 5C1
J Bacteriol 182:4394-400. 2000..By using the Fur titration assay, it was shown that the Fur box sequences upstream of fhuCBG are recognized by the Escherichia coli Fur protein...
- Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins familyA Gravet
UPRES EA 1318, Institut de Bacteriologie de la Faculte de Medecine, Université Louis Pasteur Hôpitaux Universitaires de Strasbourg, France
FEBS Lett 436:202-8. 1998..005). LukE+LukD was as effective as the Panton-Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus...
- Genetic instability of the global regulator agr explains the phenotype of the xpr mutation in Staphylococcus aureus KSI9051P J McNamara
Department of Microbiology and Immunology, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73190, USA
J Bacteriol 180:2609-15. 1998..A comparison of the nucleotide sequences of AgrC from several strains revealed seven errors in the GenBank entry for agr (X52543); these data were confirmed with plasmid pRN6650, the original wild-type clone of agr...
- MprF-mediated lysinylation of phospholipids in Staphylococcus aureus leads to protection against oxygen-independent neutrophil killingSascha A Kristian
Microbial Genetics, University of Tubingen, 72076 Tubingen, Germany
Infect Immun 71:546-9. 2003..These results demonstrate the impact and specificity of MprF-mediated CAMP resistance and underscore the role of defensin-like peptides in innate host defense...
- Glutamine synthetase and heteroresistance in methicillin-resistant Staphylococcus aureusA M Stranden
Institute of Medical Microbiology, University of Zurich, Switzerland
Microb Drug Resist 2:201-7. 1996..This suggests that S. aureus can adopt multiple ways to achieve high methicillin resistance...
- Localized perforation of the cell wall by a major autolysin: atl gene products and the onset of penicillin-induced lysis of Staphylococcus aureusM Sugai
Department of Microbiology, Hiroshima University School of Dentistry, Japan
J Bacteriol 179:2958-62. 1997....
- Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureusM Bischoff
Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
J Bacteriol 183:5171-9. 2001..The data presented here suggest that sigma(B) increases sar expression while simultaneously reducing the RNAIII level in a growth phase-dependent manner...
- Cloning and sequencing of the gene, fmtC, which affects oxacillin resistance in methicillin-resistant Staphylococcus aureusH Komatsuzawa
Department of Microbiology, Hiroshima University School of Dentistry, Kasumi 1 2 3, Minami Ku, Hiroshima City, Hiroshima 734 8553, Japan
FEMS Microbiol Lett 203:49-54. 2001..9 kDa. The N-terminal half of the deduced FmtC protein is very hydrophobic, implying that this protein is a membrane-associated protein...
- A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor AChampion C S Deivanayagam
Center for Biophysical Sciences and Engineering, School of Optometry, 244 CBSE, 1025 18th Street South, University of Alabama at Birmingham, Birmingham, AL 35294 0005, USA
EMBO J 21:6660-72. 2002....
- Expression, purification, and characterization of peptidyl-tRNA hydrolase from Staphylococcus aureusPaul D Bonin
Cell and Molecular Biology, Rockville, Maryland, USA
Protein Expr Purif 24:123-30. 2002..aureus Pth was determined to be 2.8 microM. A far UV circular dichroism spectrum revealed that His-tagged S. aureus Pth appears to have a structured core predominated by beta-sheet...
- Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like proteinMaria A Schumacher
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97201 3098, USA
EMBO J 21:3546-56. 2002..Such binding suggests a mechanism for Hfq function...
- Chorismate synthase from Staphylococcus aureusM J Horsburgh
Division of Biochemistry and Molecular Biology, University of Glasgow, UK
Microbiology 142:2943-50. 1996..The properties of S. aureus chorismate synthase are compared with those of the B. subtilis and E. coli enzymes...
- Regulatory elements of the Staphylococcus aureus protein A (Spa) promoterJinxin Gao
Department of Diagnostic Medicine Pathobiology, College of Veterinary Medicine, Kansas State University, 1800 Denison Ave, Manhattan, KS 66506, USA
J Bacteriol 186:3738-48. 2004..Full repression required the presence of a second consensus site adjacent to the SarS binding site. Sequences directly upstream of the core promoter sequence were found to stimulate transcription...
- Characterization of NorR protein, a multifunctional regulator of norA expression in Staphylococcus aureusQue Chi Truong-Bolduc
Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114 2696, USA
J Bacteriol 185:3127-38. 2003..Collectively, these results indicate that NorR is a multifunctional regulator, affecting cell surface properties as well as the expression of NorA and likely other multidrug resistance efflux pumps...
- A general method for cloning recA genes of gram-positive bacteria by polymerase chain reactionP Duwat
Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
J Bacteriol 174:5171-5. 1992..This method is particularly useful for the recovery of the recA genes of gram-positive bacteria and avoids the difficulties of using a genetic complementation test for cloning...
- Staphylococcus aureus clumping factor B (ClfB) promotes adherence to human type I cytokeratin 10: implications for nasal colonizationLouise M O'Brien
Moyne Institute of Preventive Medicine, Department of Microbiology, Trinity College Dublin, Dublin 2, Ireland
Cell Microbiol 4:759-70. 2002..We also showed that ClfB is transcribed by S. aureus in the human nares. We propose that ClfB is a major determinant in S. aureus nasal colonization...
- Transcriptional analysis of the Staphylococcus aureus penicillin binding protein 2 geneM G Pinho
Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA
J Bacteriol 180:6077-81. 1998..We now show that introduction of the intact pbp2 gene with its two newly identified promoters into the chromosome of the transposon mutant resulted in the full recovery of high-level methicillin resistance...
- A genetic and molecular characterization of the recA gene from Staphylococcus aureusK W Bayles
Department of Biological Sciences, University of Maryland, Baltimore 21228
Gene 147:13-20. 1994..Furthermore, genetic and physical mapping of S. aureus recA placed it in the same region as the uvs-568 mutation. These data strongly suggest that these mutations represent different alleles of the same recA gene...
- Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel Staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IVSusan Boyle-Vavra
Department of Pediatrics, Section of Infectious Diseases, University of Chicago, IL, 60637, USA
J Clin Microbiol 43:4719-30. 2005..SCCmec V(T) is a novel SCCmec variant that is found in multiply resistant CAMRSA strains with sequence type 59 in Taipei in association with the PVL leukotoxin genes...
- Molecular basis for the role of Staphylococcus aureus penicillin binding protein 4 in antimicrobial resistanceVikas Navratna
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India
J Bacteriol 192:134-44. 2010..Put together, the expression analysis and biochemical features of PBP4 provide a framework for understanding the function of this protein in S. aureus and its role in antimicrobial resistance...
- Reduced content of lysyl-phosphatidylglycerol in the cytoplasmic membrane affects susceptibility to moenomycin, as well as vancomycin, gentamicin, and antimicrobial peptides, in Staphylococcus aureusHiromi Nishi
Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences, Kasumi 1 2 3, Minami Ku, Hiroshima City, Hiroshima, Japan
Antimicrob Agents Chemother 48:4800-7. 2004..Therefore, the membrane charge of S. aureus appears to influence the efficacies of moenomycin, vancomycin, and other cationic antimicrobial agents...
- A nitric oxide-inducible lactate dehydrogenase enables Staphylococcus aureus to resist innate immunityAnthony R Richardson
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
Science 319:1672-6. 2008..NO.-inducible lactate dehydrogenase activity and NO. resistance distinguish S. aureus from the closely related commensal species S. epidermidis and S. saprophyticus...
- Modification of autolysis by synthetic peptides derived from the presumptive binding domain of Staphylococcus aureus autolysinM Takano
Pharmaceutical Development Research Laboratory, Tanabe Seiyaku Co, Ltd, Saitama, Japan
Microbiol Immunol 44:463-72. 2000..aureus autolysin activities. These peptides, especially the 10-mer peptide B9 (PGTKLYTVPW) that represents the C-terminal half of A10 and N-terminal half of A11, may be important segments for Atl to bind to the cell surface...
- Influence of the two-component system SaeRS on global gene expression in two different Staphylococcus aureus strainsKathrin Rogasch
Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, F L Jahn Strasse 15, D 17487 Greifswald, Germany
J Bacteriol 188:7742-58. 2006..Our results clearly show that SaeRS is strongly involved in the tight temporal control of virulence factor expression in S. aureus. Its precise role within the regulatory network remains to be determined...
- Comparison of the beta-toxins from Staphylococcus aureus and Staphylococcus intermediusK Dziewanowska
Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow 83844 3052, USA
Arch Biochem Biophys 335:102-8. 1996..Further evidence that the two proteins differ was obtained by immunological analysis which demonstrated crossreactivity but a lack of identity...
- Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strainsXiao Xue Ma
Department of Bacteriology, Juntendo University, Tokyo, Japan
Antimicrob Agents Chemother 46:1147-52. 2002..Consistent with the strains' susceptibilities to various non-beta-lactam antibiotics, the type IV SCCmec was devoid of any antibiotic resistance genes other than the mecA gene...
- Gordon Research Conference on Staphylococcal DiseasesBRIGITTE BERGER BACHI; Fiscal Year: 2003..S. This will afford a unique and rich opportunity for fellowship and scientific exchange between young and established investigators in the field. ..
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