Detection of Live Fibers in Injured Spinal Cord

Summary

Principal Investigator: Mehmet Bilgen
Abstract: [unreadable] DESCRIPTION (provided by applicant): Magnetic resonance imaging (MRI) provides in vivo 3D images of rat spinal cord (SC) with exquisite soft tissue contrast, and allows following the same animal longitudinally in experimental spinal cord injury (SCI) studies. Conventional MRIs, however, give limited information and new neuroimaging methods are required for visualizing the neural network of injured SC. Experienced with the use of MR contrast agents in our previous SCI studies, we have investigated manganese-enhanced MRI (MEI) with the potential to produce high-resolution maps of the neural fiber projections in normal and injured cords. Our initial data showed that the contrast agent manganese (Mn) spreads in both rostral and caudal directions in normal cord. In hemisectioned SCs, Mn is transported below the lesion on the contra-lateral, but not ipsilateral, to the cut. This was also confirmed by parallel studies with histological neuronal tracer. In a contusion-type SCI model, Mn labeling was detected within and below the injury, possibly due to the presence of viable tissue therein. In recent experiments, we delivered Mn into the cortex or brainstem of normal and injured rats. With intracortical injection, weak labeling was observed in the descending spinal tracts below the pyramidal decussation. But, injection to brainstem successfully produced sensitive and specific labeling in the targeted fibers. The sections of injured cord below the injury were labeled with Mn, suggesting some fibers were bridging across the injury. From these aspects, MEI offers a new tool for detecting live fibers (spared/restored) in injured SC, and may play a role in validating the accuracy of diffusion tensor imaging (DTI) in sensing these fibers. Based on these results, we hypothesize that DTI corroborated with MEI may provide crucial information on the spatiotemporal dynamics of the changes in the fiber connections during the course of recovery from SCI. To test this hypothesis, we propose to investigate normal and injured SCs using MEI, DTI and high resolution MRI along with neurobehavioral tests and end point histological analysis. These multi-modal studies will establish a new modality to probe the evolution of SCI and map the fiber projections. This should form a basis for future experimental investigations aimed at testing new therapies for promoting fiber connectivity, understanding SC plasticity and improving functional recovery from SCI. [unreadable] [unreadable] [unreadable]
Funding Period: 2006-04-20 - 2007-10-31
more information: NIH RePORT

Top Publications

  1. ncbi Preclinical use of longitudinal MRI for screening the efficacy of S-nitrosoglutathione in treating spinal cord injury
    Peter Cheng te Chou
    Preclinical Imaging in Translational Research Laboratory, Medical University of South Carolina, Charleston, South Carolina, USA
    J Magn Reson Imaging 33:1301-11. 2011
  2. ncbi Magnetic resonance imaging of contrast-enhanced polyelectrolyte complexes
    Min Huang
    Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, Kansas 66047, USA
    Nanomedicine 4:30-40. 2008
  3. pmc Evaluating regional blood spinal cord barrier dysfunction following spinal cord injury using longitudinal dynamic contrast-enhanced MRI
    Ilkan Tatar
    Preclinical Imaging in Translational Research Laboratory, Radiology and Radiological Science, Medical University of South Carolina, 169 Ashley Avenue, Charleston, SC 29425, USA
    BMC Med Imaging 9:10. 2009

Scientific Experts

  • Ilkan Tatar
  • Peter Cheng te Chou
  • Mehmet Bilgen
  • Min Huang
  • Inderjit Singh
  • Anandakumar Shunmugavel
  • Mohamed Mokhtar Desouki
  • Mushfiquddin Khan
  • Shaun A Nguyen
  • Hanaa El Sayed
  • Cory Berkland
  • Zhixin L Huang

Detail Information

Publications3

  1. ncbi Preclinical use of longitudinal MRI for screening the efficacy of S-nitrosoglutathione in treating spinal cord injury
    Peter Cheng te Chou
    Preclinical Imaging in Translational Research Laboratory, Medical University of South Carolina, Charleston, South Carolina, USA
    J Magn Reson Imaging 33:1301-11. 2011
    ..To evaluate the therapeutic efficacy of S-nitrosoglutathione (GSNO) in spinal cord injury (SCI) using in vivo MRI in combination with neuorobehavioral testing and postmortem tissue analysis...
  2. ncbi Magnetic resonance imaging of contrast-enhanced polyelectrolyte complexes
    Min Huang
    Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, Kansas 66047, USA
    Nanomedicine 4:30-40. 2008
    ..MRI detection of PECs provides a potential approach to rapidly evaluate parameters such as the biodistribution and pharmacokinetics of these established drug and gene delivery vehicles...
  3. pmc Evaluating regional blood spinal cord barrier dysfunction following spinal cord injury using longitudinal dynamic contrast-enhanced MRI
    Ilkan Tatar
    Preclinical Imaging in Translational Research Laboratory, Radiology and Radiological Science, Medical University of South Carolina, 169 Ashley Avenue, Charleston, SC 29425, USA
    BMC Med Imaging 9:10. 2009
    ..This paper uses multimodal magnetic resonance imaging (MRI) to investigate neurovascular pathology and changes in blood spinal cord barrier (BSCB) permeability following SCI in a mouse model of SCI...