In Vivo Measurement of Optic Nerve Axon Loss
Principal Investigator: JAMES LINDSEY
Abstract: [unreadable] DESCRIPTION: The overall aim of this project is to establish a new method for obtaining repeatable quantitative measurements of retinal ganglion cell projections to the mouse brain in vivo. This will allow direct longitudinal assessment of optic nerve axon projections to the brain in mouse models of ocular disease characterized by gradual optic nerve degeneration. Aim 1. To correlate the distribution and signal strength of an orthograde axonal tracer recognizable in MRI images with the number of axons present in the optic nerve. Using normal mice and mice that previously received graded optic nerve crush, these studies will correlate the strength of the MRI signal and the amount of tracer injected into the eye with the number of optic nerve axons as determined by electron microscopy. Aim 2. To define the limitations of making multiple injections of the MRI tracer over time in order to obtain longitudinal assessments of optic nerve axon projections. These studies will evaluate the limits of experimental parameters within which one to four reproducible repeated optic nerve projection measurements can be made without inducing retinal or optic nerve damage. Experiments will be conducted in normal mice and mice that previously received graded optic nerve crush. They will include assessments of neuronal survival, neuronal morphology, and gliosis in the retina and in retinorecipient nuclei of the brain. Aim 3. To assess the validity of the optimized parameters for the evaluation of optic nerve axon loss in a transgenic spontaneous mouse model of open angle glaucoma. These studies will correlate the distribution and signal strength of an orthograde axonal tracer recognizable in MRI images with the [unreadable] distribution and strength of a standard histological orthograde axonal tracer and the number of axons [unreadable] present in the optic nerve. [unreadable] [unreadable]
Funding Period: 2006-05-15 - 2009-04-30
more information: NIH RePORT
- In vivo imaging of murine retinal ganglion cellsChristopher K S Leung
Hamilton Glaucoma Center, Department of Ophthalmology, University of California, San Diego, La Jolla, CA, United States
J Neurosci Methods 168:475-8. 2008....
- Longitudinal profile of retinal ganglion cell damage after optic nerve crush with blue-light confocal scanning laser ophthalmoscopyChristopher Kai Shun Leung
Department of Ophthalmology, Hamilton Glaucoma Center, University of California San Diego, La Jolla, California 92093 0946, USA
Invest Ophthalmol Vis Sci 49:4898-902. 2008..To investigate the long-term longitudinal profile of retinal ganglion cell (RGC) damage after optic nerve crush with a new technique for in vivo imaging of RGCs...
- Oxidative stress is an early event in hydrostatic pressure induced retinal ganglion cell damageQuan Liu
Hamilton Glaucoma Center and the Department of Ophthalmology, University of California San Diego, La Jolla, California 92037 0946, USA
Invest Ophthalmol Vis Sci 48:4580-9. 2007..To determine whether oxidative adduct formation or heme oxygenase-1 (HO-1) expression are altered in retinal ganglion cell (RGC) cultures exposed to elevated hydrostatic pressure and in a mouse model of glaucoma...