NANOPOROUS INOGANIC BIOCAPSULES

Summary

Principal Investigator: Tejal A Desai
Abstract: The ability to create well-defined and controlled interfaces has been an area of great interest over the last few years, particularly in the biomedical arena. The goal of this research is to use innovative materials synthesis strategies to create both passive, and active, precision bioseparation membranes. Of particular interest to them is the development and characterization of well-controlled, stable, and uniform nano-dimensional membranes capable of the separation of viruses and/or proteins during the blood fractionation process and the blocking of antibodies and complement molecules from encapsulated xenogeneic cells. In such applications, the leakage of just one virus or antibody molecule through the membrane will compromise the entire system. It is hypothesized that high surface area cylindrical capsules, the walls of which are comprised of nanoporous membranes, created via electric-field driven anodization of aluminum or titanium can be used for the absolute filtration or exclusion of biomolecules in the nanometer range. Beyond making passive membranes, under the program auspices, nanoporous biocapsules incorporating magnetoelastic elements will be fabricated. The magnetoelastic elements enable the biocapsule to be mechanically vibrated, remotely from a distance, by application of a time varying magnetic field facilitating, and they believe ultimately allowing one to control, transport through the membrane. The proposed research project will focus on defining optimal routes for the fabrication of nanoporous capsules and the characterization of the material/structural properties of the nanoporous membranes with attention to film optimization and the functionality of the membranes as biological filters. The application of passive nanoporous biocapsules for cellular encapsulation/immunoisolation and magnetoelastic mechanically-active biocapsules for controlled transport through the nanoporous membranes will be investigated.
Funding Period: 2002-09-30 - 2005-02-28
more information: NIH RePORT

Top Publications

  1. pmc Biocompatibility of nanoporous alumina membranes for immunoisolation
    Kristen E La Flamme
    Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
    Biomaterials 28:2638-45. 2007
  2. pmc Inorganic nanoporous membranes for immunoisolated cell-based drug delivery
    Adam Mendelsohn
    UCSF UCB Joint Graduate Group in Bioengineering, University of California, San Francisco, University of California, Berkeley, USA
    Adv Exp Med Biol 670:104-25. 2010
  3. ncbi Peptide-immobilized nanoporous alumina membranes for enhanced osteoblast adhesion
    Erin E Leary Swan
    Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, MA 02215, USA
    Biomaterials 26:1969-76. 2005
  4. ncbi Fabrication and evaluation of nanoporous alumina membranes for osteoblast culture
    Erin E Leary Swan
    Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA
    J Biomed Mater Res A 72:288-95. 2005
  5. ncbi Influence of nanoporous alumina membranes on long-term osteoblast response
    Ketul C Popat
    Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, MA 02215, USA
    Biomaterials 26:4516-22. 2005
  6. ncbi Modeling of RGDC film parameters using X-ray photoelectron spectroscopy
    Ketul C Popat
    Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA
    Langmuir 21:7061-5. 2005

Detail Information

Publications6

  1. pmc Biocompatibility of nanoporous alumina membranes for immunoisolation
    Kristen E La Flamme
    Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
    Biomaterials 28:2638-45. 2007
    ..Further, in vivo work has demonstrated that implantation of these capsules into the peritoneal cavity of rats induces a transient inflammatory response, and that PEG is useful in minimizing the host response to the material...
  2. pmc Inorganic nanoporous membranes for immunoisolated cell-based drug delivery
    Adam Mendelsohn
    UCSF UCB Joint Graduate Group in Bioengineering, University of California, San Francisco, University of California, Berkeley, USA
    Adv Exp Med Biol 670:104-25. 2010
    ..This review outlines the challenges involved with maintaining transplanted cell viability and discusses how inorganic nanoporous membranes may be useful in achieving clinical success...
  3. ncbi Peptide-immobilized nanoporous alumina membranes for enhanced osteoblast adhesion
    Erin E Leary Swan
    Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, MA 02215, USA
    Biomaterials 26:1969-76. 2005
    ..Cell secreted matrix was absent on unmodified membranes for the same duration. Vitronectin-adsorbed surfaces did not show significant improvement in adhesion over unmodified membranes...
  4. ncbi Fabrication and evaluation of nanoporous alumina membranes for osteoblast culture
    Erin E Leary Swan
    Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA
    J Biomed Mater Res A 72:288-95. 2005
    ....
  5. ncbi Influence of nanoporous alumina membranes on long-term osteoblast response
    Ketul C Popat
    Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, MA 02215, USA
    Biomaterials 26:4516-22. 2005
    ..Results indicate improved osteoblast adhesion and proliferation and increased matrix production after 4 weeks of study...
  6. ncbi Modeling of RGDC film parameters using X-ray photoelectron spectroscopy
    Ketul C Popat
    Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA
    Langmuir 21:7061-5. 2005
    ..These data were further utilized to calculate RGDC film thickness, density, and coverage, which are essential for understanding bone cell interactions with the underlying films and substrate...