Cell-and Nucleus-Permeable Luminescent Gold Nanoparticles

Summary

Principal Investigator: Jie Zheng
Abstract: Intracellular imaging and targeted delivery demand new nanomaterials that can directly cross cell membranes and enter the cytosol without being encapsulated by membrane bound vesicles. We recently found that highly luminescent, gold nanoparticles about 2 nm in diameter when exposed to HeLa cells rapidly crossed cellular membranes and nuclear membranes apparently by passive diffusion and subsequently bound to chromosomes in live cells. The objective of this application is to unravel underlying principles that govern the cell/nuclear permeability of nanomaterials. To achieve this goal, we proposed to develop general approaches that allow us to create a library of gold nanoprobes with fine tuned surface chemistry and structure, and then apply fluorescence microscopy techniques to investigate cell behaviors of these nanomaterials. Successful realization of these aims will greatly advance our understanding of fundamental principles that regulate the permeability of nanostructures at the molecular level. These studies will be further broadened beyond the scope of this exploratory period and lead to development of new nanomedicines for intracellular imaging and targeted delivery. PUBLIC HEALTH RELEVANCE: Cell- and Nucleus-Permeable Luminescent Gold Nanoparticles Nanomaterials generally cannot cross cell /nuclear membranes via passive diffusion, but are taken up by the cell via endocytosis. We recently found a luminescent gold nanostructure can directly diffuse across cell and nuclear membranes within a few minutes. We propose to develop a library of luminescent gold nanoprobes with different surface properties, so that we can uncover the underlying principles that govern cell and nuclear permeability of gold nanoparticles. 1
Funding Period: ----------------2009 - ---------------2011-
more information: NIH RePORT

Top Publications

  1. pmc One-step interfacial synthesis and assembly of ultrathin luminescent AuNPs/silica membranes
    Shengyang Yang
    Department of Chemistry, The University of Texas at Dallas, Richardson, 75080, USA
    Adv Mater 24:3218-22. 2012
  2. pmc Different sized luminescent gold nanoparticles
    Jie Zheng
    Department of Chemistry, University of Texas at Dallas, 800 W Campbell Rd, Richardson, TX 75080, USA
    Nanoscale 4:4073-83. 2012
  3. ncbi Near-infrared emitting radioactive gold nanoparticles with molecular pharmacokinetics
    Chen Zhou
    Department of Chemistry, The University of Texas at Dallas, 800 W Campbell Road, Richardson, TX 75080 USA http www utdallas edu jiezheng
    Angew Chem Int Ed Engl 51:10118-22. 2012
  4. ncbi Luminescent gold nanoparticles: a new class of nanoprobes for biomedical imaging
    Chen Zhou
    Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75080, USA
    Exp Biol Med (Maywood) 238:1199-209. 2013
  5. pmc PEGylation and Zwitterionization: Pros and Cons in the Renal Clearance and Tumor Targeting of Near-IR-Emitting Gold Nanoparticles
    Jinbin Liu
    Department of Chemistry, The University of Texas at Dallas, 800 W Campbell Rd Richardson, Texas, 75080 USA http www utdallas edu jiezheng
    Angew Chem Int Ed Engl 52:12572-6. 2013
  6. ncbi Synthesis of gold nanoclusters: a fluorescent marker for water-soluble TiO2 nanotubes
    Chalita Ratanatawanate
    Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75080 3021, USA
    Nanotechnology 22:065601. 2011
  7. pmc Luminescent gold nanoparticles with pH-dependent membrane adsorption
    Mengxiao Yu
    Department of Chemistry, The University of Texas at Dallas, Richardson, Texas 75080, USA
    J Am Chem Soc 133:11014-7. 2011

Detail Information

Publications9

  1. pmc One-step interfacial synthesis and assembly of ultrathin luminescent AuNPs/silica membranes
    Shengyang Yang
    Department of Chemistry, The University of Texas at Dallas, Richardson, 75080, USA
    Adv Mater 24:3218-22. 2012
    ..Such a versatile concept is ideal for the development of multifunctional devices...
  2. pmc Different sized luminescent gold nanoparticles
    Jie Zheng
    Department of Chemistry, University of Texas at Dallas, 800 W Campbell Rd, Richardson, TX 75080, USA
    Nanoscale 4:4073-83. 2012
    ..In this review, we summarized the strategies used to create luminescent gold nanoparticles with sizes from few to millions of atoms and discussed how these structural factors affect their photoluminescence...
  3. ncbi Near-infrared emitting radioactive gold nanoparticles with molecular pharmacokinetics
    Chen Zhou
    Department of Chemistry, The University of Texas at Dallas, 800 W Campbell Road, Richardson, TX 75080 USA http www utdallas edu jiezheng
    Angew Chem Int Ed Engl 51:10118-22. 2012
    ..These nanoparticles show a rapid distribution half-life, a desirable elimination half-life, and hold promise for single-photon emission computed tomography (SPECT) and fluorescence imaging...
  4. ncbi Luminescent gold nanoparticles: a new class of nanoprobes for biomedical imaging
    Chen Zhou
    Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75080, USA
    Exp Biol Med (Maywood) 238:1199-209. 2013
    ..In this minireview, we summarize the recent progress in applications of different-sized luminescent AuNPs as imaging probes for both in vitro and in vivo levels. ..
  5. pmc PEGylation and Zwitterionization: Pros and Cons in the Renal Clearance and Tumor Targeting of Near-IR-Emitting Gold Nanoparticles
    Jinbin Liu
    Department of Chemistry, The University of Texas at Dallas, 800 W Campbell Rd Richardson, Texas, 75080 USA http www utdallas edu jiezheng
    Angew Chem Int Ed Engl 52:12572-6. 2013
    ..High tumor-targeting efficiency and specificity were obtained with PEGylated AuNPs, whereas rapid tumor detection was more readily achieved with zwitterionic AuNPs. HD=hydrodynamic diameter, GS=glutathione. ..
  6. ncbi Synthesis of gold nanoclusters: a fluorescent marker for water-soluble TiO2 nanotubes
    Chalita Ratanatawanate
    Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75080 3021, USA
    Nanotechnology 22:065601. 2011
    ..The gold clusters emit at 660 nm in water and were imaged using confocal microscopy. The gold decorated TNTs were suspended in water by wrapping the nanotubes with poly-L-arginine...
  7. pmc Luminescent gold nanoparticles with pH-dependent membrane adsorption
    Mengxiao Yu
    Department of Chemistry, The University of Texas at Dallas, Richardson, Texas 75080, USA
    J Am Chem Soc 133:11014-7. 2011
    ....