Cell-and Nucleus-Permeable Luminescent Gold Nanoparticles
Principal Investigator: Jie Zheng
Abstract: Intracellular imaging and targeted delivery demand new nanomaterials that can directly cross cell membranes and enter the cytosol without being encapsulated by membrane bound vesicles. We recently found that highly luminescent, gold nanoparticles about 2 nm in diameter when exposed to HeLa cells rapidly crossed cellular membranes and nuclear membranes apparently by passive diffusion and subsequently bound to chromosomes in live cells. The objective of this application is to unravel underlying principles that govern the cell/nuclear permeability of nanomaterials. To achieve this goal, we proposed to develop general approaches that allow us to create a library of gold nanoprobes with fine tuned surface chemistry and structure, and then apply fluorescence microscopy techniques to investigate cell behaviors of these nanomaterials. Successful realization of these aims will greatly advance our understanding of fundamental principles that regulate the permeability of nanostructures at the molecular level. These studies will be further broadened beyond the scope of this exploratory period and lead to development of new nanomedicines for intracellular imaging and targeted delivery. PUBLIC HEALTH RELEVANCE: Cell- and Nucleus-Permeable Luminescent Gold Nanoparticles Nanomaterials generally cannot cross cell /nuclear membranes via passive diffusion, but are taken up by the cell via endocytosis. We recently found a luminescent gold nanostructure can directly diffuse across cell and nuclear membranes within a few minutes. We propose to develop a library of luminescent gold nanoprobes with different surface properties, so that we can uncover the underlying principles that govern cell and nuclear permeability of gold nanoparticles. 1
Funding Period: ----------------2009 - ---------------2011-
more information: NIH RePORT
- One-step interfacial synthesis and assembly of ultrathin luminescent AuNPs/silica membranesShengyang Yang
Department of Chemistry, The University of Texas at Dallas, Richardson, 75080, USA
Adv Mater 24:3218-22. 2012..Such a versatile concept is ideal for the development of multifunctional devices...
- Different sized luminescent gold nanoparticlesJie Zheng
Department of Chemistry, University of Texas at Dallas, 800 W Campbell Rd, Richardson, TX 75080, USA
Nanoscale 4:4073-83. 2012..In this review, we summarized the strategies used to create luminescent gold nanoparticles with sizes from few to millions of atoms and discussed how these structural factors affect their photoluminescence...
- Near-infrared emitting radioactive gold nanoparticles with molecular pharmacokineticsChen Zhou
Department of Chemistry, The University of Texas at Dallas, 800 W Campbell Road, Richardson, TX 75080 USA http www utdallas edu jiezheng
Angew Chem Int Ed Engl 51:10118-22. 2012..These nanoparticles show a rapid distribution half-life, a desirable elimination half-life, and hold promise for single-photon emission computed tomography (SPECT) and fluorescence imaging...
- Luminescent gold nanoparticles: a new class of nanoprobes for biomedical imagingChen Zhou
Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75080, USA
Exp Biol Med (Maywood) 238:1199-209. 2013..In this minireview, we summarize the recent progress in applications of different-sized luminescent AuNPs as imaging probes for both in vitro and in vivo levels. ..
- PEGylation and Zwitterionization: Pros and Cons in the Renal Clearance and Tumor Targeting of Near-IR-Emitting Gold NanoparticlesJinbin Liu
Department of Chemistry, The University of Texas at Dallas, 800 W Campbell Rd Richardson, Texas, 75080 USA http www utdallas edu jiezheng
Angew Chem Int Ed Engl 52:12572-6. 2013..High tumor-targeting efficiency and specificity were obtained with PEGylated AuNPs, whereas rapid tumor detection was more readily achieved with zwitterionic AuNPs. HD=hydrodynamic diameter, GS=glutathione. ..
- Synthesis of gold nanoclusters: a fluorescent marker for water-soluble TiO2 nanotubesChalita Ratanatawanate
Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75080 3021, USA
Nanotechnology 22:065601. 2011..The gold clusters emit at 660 nm in water and were imaged using confocal microscopy. The gold decorated TNTs were suspended in water by wrapping the nanotubes with poly-L-arginine...
- Luminescent gold nanoparticles with pH-dependent membrane adsorptionMengxiao Yu
Department of Chemistry, The University of Texas at Dallas, Richardson, Texas 75080, USA
J Am Chem Soc 133:11014-7. 2011....