Noninvasive Functional and Cellular Imaging of Epilepsy

Summary

Principal Investigator: Huabei Jiang
Abstract: DESCRIPTION (provided by applicant): Approximately 2.5 million Americans live with epilepsy and epilepsy-related deficits today, more than disabled by Parkinson disease or brain tumors. The impact of epilepsy in the US is significant with a total cost to the nation for seizures and epilepsy of approximately $12.5 billion. Epilepsy consists of more than 40 clinical syndromes affecting 40 million people worldwide. Approximately 25 percent of individuals receiving antiepileptic medication have inadequate seizure control;however, 80% individuals with medication resistant epilepsy might be cured through surgery if one were able to precisely localize the seizure focus. The proposed research will significantly advance our ability to localize such foci, and thereby offer curative epilepsy surgery for this devastating disease. We hypothesize that diffuse optical tomography (DOT) provides a new functional and cellular neuroimaging modality for non-invasively tracking dynamical changes during seizure occurrence. DOT has the ability to image tissue functions including oxyhemoglobin (HbO2), deoxyhemoglobin (Hb), cerebral blood volume (CBV), cerebral blood flow (CBF) and rate of cerebral oxygen metabolism (CMRO2). In addition, we show that DOT can also provide cerebral cellular morphology that is derived from tissue scattering spectra. Given the high temporal and spatial resolution for optical modalities, the proposed hemodynamic and cellular imaging will offer unprecedented localization of both ictal and interictal seizure activities, a feature that is unavailable from any of the existing neuroimaging techniques. While the primary goal of this project is to develop a DOT system for accurate localization of epileptic focus, we also propose to study the neuro-vascular and neuro-cellular coupling between neuronal activity and hemodynamic/cellular response using concurrent electroencephalography (EEG) and DOT noninvasively in animal models of seizure. Such coupling study is becoming increasingly important for interpreting functional/cellular imaging results, and may be useful in predicting seizure onset. To test the hypothesis, we propose to complete the following specific aims: (1) To design, construct and test a fast multispectral optical system for functional/cellular DOT imaging;(2) To develop a set of image enhancement schemes for improved DOT imaging of small animals, and To develop both linear and nonlinear models that couple the electrophysiological recordings with the hemodynamic/cellular response measured by DOT;(3) To evaluate and optimize the integrated functioning of the hardware and software components of the multispectral DOT system, using simulation and phantom experiments;(4) To test and evaluate the proposed DOT system and neuro-vascular/neuro-cellular coupling models using animal models. PUBLIC HEALTH RELEVANCE: Many individuals with medication resistant epilepsy might be cured through surgery if one were able to precisely localize the seizure focus. The proposed research will significantly advance our ability to localize such foci by developing a new imaging technology, and thereby offer curative epilepsy surgery for this devastating disease. Both technological development and in vivo animal studies are proposed in this application.
Funding Period: 2010-02-15 - 2015-01-31
more information: NIH RePORT

Top Publications

  1. pmc T-matrix-based inverse algorithm for morphologic characterization of nonspherical particles using multispectral diffuse optical tomography
    M Reza Hajihashemi
    Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA
    Appl Opt 50:3896-906. 2011
  2. pmc Pre-seizure state identified by diffuse optical tomography
    Tao Zhang
    J Crayton Pruitt Family Department of Biomedical Engineering, University of Florida Gainesville, FL 32611, USA
    Sci Rep 4:3798. 2014
  3. pmc A calibration-free, one-step method for quantitative photoacoustic tomography
    Zhen Yuan
    Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA
    Med Phys 39:6895-9. 2012
  4. pmc Noninvasive evaluation of nuclear morphometry in breast lesions using multispectral diffuse optical tomography
    Mohammad Reza Hajihashemi
    Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA
    PLoS ONE 7:e45714. 2012
  5. pmc Fast multispectral diffuse optical tomography system for in vivo three-dimensional imaging of seizure dynamics
    Jianjun Yang
    Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA
    Appl Opt 51:3461-9. 2012
  6. pmc An inverse light scattering technique for morphological characterization of irregular particles based on the Gaussian-random-sphere model
    M Reza Hajihashemi
    Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA
    J Opt Soc Am A Opt Image Sci Vis 29:1124-31. 2012
  7. pmc Morphologic tomography of nonspherical particles using multispectral diffusing light measurements
    Mohammad Reza Hajihashemi
    University of Florida, Department of Biomedical Engineering, Gainesville, Florida 32611 6131, USA
    J Biomed Opt 16:116014. 2011
  8. ncbi Non-invasive detection of optical changes elicited by seizure activity using time-series analysis of light scattering images in a rat model of generalized seizure
    M Reza Hajihashemi
    Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States
    J Neurosci Methods 227:18-28. 2014

Detail Information

Publications10

  1. pmc T-matrix-based inverse algorithm for morphologic characterization of nonspherical particles using multispectral diffuse optical tomography
    M Reza Hajihashemi
    Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA
    Appl Opt 50:3896-906. 2011
    ..Different statistical models are assumed for the suspended nonspherical particles and the performance of the inverse algorithm is tested using noise-corrupted data up to 50% noise added to the observed scattering spectra...
  2. pmc Pre-seizure state identified by diffuse optical tomography
    Tao Zhang
    J Crayton Pruitt Family Department of Biomedical Engineering, University of Florida Gainesville, FL 32611, USA
    Sci Rep 4:3798. 2014
    ..Together, these findings suggest DOT represents a powerful tool for mapping early seizure onset and propagation pathways. ..
  3. pmc A calibration-free, one-step method for quantitative photoacoustic tomography
    Zhen Yuan
    Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA
    Med Phys 39:6895-9. 2012
    ..The purpose of this study is to develop a new algorithm for the reconstruction of optical absorption coefficient that does not depend on these initial parameters...
  4. pmc Noninvasive evaluation of nuclear morphometry in breast lesions using multispectral diffuse optical tomography
    Mohammad Reza Hajihashemi
    Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA
    PLoS ONE 7:e45714. 2012
    ..This pilot study demonstrates the potential of multispectral DOT as a cellular imaging method for accurate determination of breast cancer...
  5. pmc Fast multispectral diffuse optical tomography system for in vivo three-dimensional imaging of seizure dynamics
    Jianjun Yang
    Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA
    Appl Opt 51:3461-9. 2012
    ..The system is validated using both static and dynamic tissue-like phantoms. An initial in vivo experiment using a rat model of seizure is also demonstrated...
  6. pmc An inverse light scattering technique for morphological characterization of irregular particles based on the Gaussian-random-sphere model
    M Reza Hajihashemi
    Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA
    J Opt Soc Am A Opt Image Sci Vis 29:1124-31. 2012
    ..The performance of the inverse algorithm is tested using noise-corrupted data, in which up to 50% noise may be added to the observed scattering spectra...
  7. pmc Morphologic tomography of nonspherical particles using multispectral diffusing light measurements
    Mohammad Reza Hajihashemi
    University of Florida, Department of Biomedical Engineering, Gainesville, Florida 32611 6131, USA
    J Biomed Opt 16:116014. 2011
    ..Diffusely scattered light at several wavelengths along the boundary of the phantom are collected and analyzed to allow for simultaneous extraction of the size, concentration, and aspect ratio of the spheroidal particles...
  8. ncbi Non-invasive detection of optical changes elicited by seizure activity using time-series analysis of light scattering images in a rat model of generalized seizure
    M Reza Hajihashemi
    Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States
    J Neurosci Methods 227:18-28. 2014
    ....