Functional Assessment of Pulmonary Toxicity with MRM

Summary

Principal Investigator: G Johnson
Abstract: DESCRIPTION (Provided by Applicant): We propose continuation of studies of the environmental impact of particulate matter on lung structure and function using in vivo magnetic resonance microscopy. We will expand our previous work with hyperpolarized 3He to support localized three-dimensional structural imaging at 1 x 10-4 mm3. We will extend our physiologic support system and spiral encoding techniques to quantitative flow measurements and functional imaging at 1 x 10-2 mm3. We will study the impact of particulates in three specific models: 1) elastase-induced injury, a model of emphysema; 2) inflammation and remodeling in the endotoxin sensitive C3H/HeBFeJ mouse, a model of organic dust induced asthma; and 3) inflammation and remodeling in the vanadium pentoxide model of asthma, which is characterized by extensive bronchiolar fibrosis. The proposal will refine methods that will be applicable to a much wider range of basic pulmonary studies while obtaining detailed assessment of structural and functional changes in three important models of human disease. By extending the methodologies to the mouse, we will build a critical bridge between man and mouse models of pulmonary disease that will be essential to understanding the pathophysiology of pulmonary disease and the validation of new therapies.
Funding Period: 1996-09-30 - 2006-08-31
more information: NIH RePORT

Top Publications

  1. ncbi Ventilation-synchronous magnetic resonance microscopy of pulmonary structure and ventilation in mice
    Ben T Chen
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, NC 27710, USA
    Magn Reson Med 53:69-75. 2005
  2. pmc Small animal imaging with magnetic resonance microscopy
    Bastiaan Driehuys
    Department of Radiology, Center for In Vivo Microscopy, Duke University Medical Center, Durham, NC 27710, USA
    ILAR J 49:35-53. 2008
  3. pmc 3He MRI in mouse models of asthma
    Bastiaan Driehuys
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, North Carolina 27710, USA
    Magn Reson Med 58:893-900. 2007
  4. pmc Cardiac micro-computed tomography for morphological and functional phenotyping of muscle LIM protein null mice
    Cristian T Badea
    Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Durham, NC, USA
    Mol Imaging 6:261-8. 2007
  5. pmc Imaging techniques for small animal models of pulmonary disease: MR microscopy
    Bastiaan Driehuys
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, North Carolina 27710, USA
    Toxicol Pathol 35:49-58. 2007
  6. pmc High-resolution imaging of murine myocardial infarction with delayed-enhancement cine micro-CT
    Matthias Nahrendorf
    Center for Molecular Imaging Research, Massachusetts General Hospital, Boston, MA, USA
    Am J Physiol Heart Circ Physiol 292:H3172-8. 2007
  7. pmc Imaging alveolar-capillary gas transfer using hyperpolarized 129Xe MRI
    Bastiaan Driehuys
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 103:18278-83. 2006
  8. ncbi Imaging methods for morphological and functional phenotyping of the rodent heart
    Cristian T Badea
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, North Carolina 27710, USA
    Toxicol Pathol 34:111-7. 2006
  9. pmc Morphology of the small-animal lung using magnetic resonance microscopy
    Laurence W Hedlund
    Center for In Vivo Microscopy, Box 3302, Duke University Medical Center, Durham, NC 27710, USA
    Proc Am Thorac Soc 2:481-3, 501-2. 2005
  10. ncbi Automated segmentation of neuroanatomical structures in multispectral MR microscopy of the mouse brain
    Anjum A Ali
    Center for In Vivo Microscopy, Box 3302, Duke University Medical Center, Durham, NC 27710, USA
    Neuroimage 27:425-35. 2005

Scientific Experts

Detail Information

Publications12

  1. ncbi Ventilation-synchronous magnetic resonance microscopy of pulmonary structure and ventilation in mice
    Ben T Chen
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, NC 27710, USA
    Magn Reson Med 53:69-75. 2005
    ..2 mL/breath. The feasibility of acquiring both pulmonary vasculature and ventilated airways was demonstrated in the mouse lung with in-plane spatial resolution of 70 x 70 microm(2) and slice thickness of 800 microm...
  2. pmc Small animal imaging with magnetic resonance microscopy
    Bastiaan Driehuys
    Department of Radiology, Center for In Vivo Microscopy, Duke University Medical Center, Durham, NC 27710, USA
    ILAR J 49:35-53. 2008
    ..The ability of MRM to provide a detailed functional and anatomical picture in rats and mice, and to track this picture over time, makes it a promising platform with broad applications in biomedical research...
  3. pmc 3He MRI in mouse models of asthma
    Bastiaan Driehuys
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, North Carolina 27710, USA
    Magn Reson Med 58:893-900. 2007
    ..The extension of (3)He MRI to mouse models should make it a valuable translational tool in asthma research...
  4. pmc Cardiac micro-computed tomography for morphological and functional phenotyping of muscle LIM protein null mice
    Cristian T Badea
    Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Durham, NC, USA
    Mol Imaging 6:261-8. 2007
    ..Other sources of errors for micro-CT are also analyzed. Micro-CT can provide the four-dimensional data (three-dimensional isotropic volumes over time) required for morphological and functional phenotyping in mice...
  5. pmc Imaging techniques for small animal models of pulmonary disease: MR microscopy
    Bastiaan Driehuys
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, North Carolina 27710, USA
    Toxicol Pathol 35:49-58. 2007
    ..MRM has become a valuable tool for studying morphologic and functional changes in small animal models of lung diseases...
  6. pmc High-resolution imaging of murine myocardial infarction with delayed-enhancement cine micro-CT
    Matthias Nahrendorf
    Center for Molecular Imaging Research, Massachusetts General Hospital, Boston, MA, USA
    Am J Physiol Heart Circ Physiol 292:H3172-8. 2007
    ..This efficient imaging tool is a valuable addition to the current phenotyping armamentarium and will allow rapid testing of novel drugs and cell-based interventions in murine models...
  7. pmc Imaging alveolar-capillary gas transfer using hyperpolarized 129Xe MRI
    Bastiaan Driehuys
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 103:18278-83. 2006
    ..We validate the successful separation of tissue barrier and RBC images and show the utility of this method in a rat model of pulmonary fibrosis where (129)Xe replenishment of the RBCs is severely impaired in regions of lung injury...
  8. ncbi Imaging methods for morphological and functional phenotyping of the rodent heart
    Cristian T Badea
    Center for In Vivo Microscopy, Duke University Medical Center, Durham, North Carolina 27710, USA
    Toxicol Pathol 34:111-7. 2006
    ....
  9. pmc Morphology of the small-animal lung using magnetic resonance microscopy
    Laurence W Hedlund
    Center for In Vivo Microscopy, Box 3302, Duke University Medical Center, Durham, NC 27710, USA
    Proc Am Thorac Soc 2:481-3, 501-2. 2005
    ..With further improvements in imaging and animal handling technology, we will be able to image faster and at higher resolutions, making MRM an even more valuable research tool...
  10. ncbi Automated segmentation of neuroanatomical structures in multispectral MR microscopy of the mouse brain
    Anjum A Ali
    Center for In Vivo Microscopy, Box 3302, Duke University Medical Center, Durham, NC 27710, USA
    Neuroimage 27:425-35. 2005
    ..Results achieved in the mouse brain are comparable with those achieved in human brain studies using similar techniques. The segmentation algorithm shows excellent potential for routine morphological phenotyping of mouse models...
  11. ncbi Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents
    Wilfried Mai
    Center for In Vivo Microscopy, Box 3302, Duke University Medical Center, Durham, NC 27710, USA
    Magn Reson Med 53:858-65. 2005
    ..With careful control of motion, its impact on resolution can be limited. This work provides the first quantitative measure of the motion-imposed resolution limits for in vivo imaging...
  12. pmc T1 relaxivity of core-encapsulated gadolinium liposomal contrast agents--effect of liposome size and internal gadolinium concentration
    Ketan Ghaghada
    Department of Radiology, Box 3808, Duke University Medical Center, Durham, NC 27710, USA
    Acad Radiol 15:1259-63. 2008
    ..The objective of this study was to investigate the effect of liposome size and internal (core) Gd concentration on the T1 relaxivity of CE-Gd liposomes...