RNA EDITING IN PLANT ORGANELLES

Summary

Principal Investigator: Maureen Hanson
Abstract: RNA editing alters the predicted nuclear or organelle gene products from many organisms, ranging from protozoans to humans. This distinctive form of RNA processing gives organisms unique flexibility in producing messenger and structural RNAs and proteins from DNA genomes. For organelles of some plants and animals, RNA editing can be viewed as a correction mechanism for genes that otherwise could not be expressed or would encode aberrant products. RNA editing also sometimes serves to allow the production of proteins with diverse functions or properties from a single gene. In humans, transcripts of genes involved in lipid metabolism and nervous system function are among those that undergo base modifications. Fundamental aspects of base modification RNA editing will be uncovered by studies of C-to-U RNA editing in plastids of angiosperm plants. Unlike other organelles in which RNA editing occurs plastids can be transformed with deliberately altered editing sites to analyze the cis-acting elements necessary for catalysis and recognition of bases for modification. Mutant screens will identify nuclear or organelle genes which affect editing site recognition and editing efficiency. With information obtained from transformation analysis, substrate RNAs can be designed to identify components of the editing apparatus that associate with target transcripts. The proposed research should enhance understanding of other forms of RNA processing in the biological world, especially the mechanisms of tRNA, mRNA and rRNA modification and editing.
Funding Period: 1994-05-01 - 2003-11-30
more information: NIH RePORT

Top Publications

  1. pmc Expression of complementary RNA from chloroplast transgenes affects editing efficiency of transgene and endogenous chloroplast transcripts
    Carla E Hegeman
    Department of Molecular Biology and Genetics, Cornell University Ithaca, NY 14853, USA
    Nucleic Acids Res 33:1454-64. 2005
  2. ncbi Substrate and cofactor requirements for RNA editing of chloroplast transcripts in Arabidopsis in vitro
    Carla E Hegeman
    Department of Molecular Biology and Genetics, Cornell University, Biotechnology Building, Ithaca, NY 14853, USA
    Plant J 42:124-32. 2005
  3. pmc Ecotype allelic variation in C-to-U editing extent of a mitochondrial transcript identifies RNA-editing quantitative trait loci in Arabidopsis
    Stephane Bentolila
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Plant Physiol 139:2006-16. 2005

Scientific Experts

  • Maureen Hanson
  • Carla E Hegeman
  • Stephane Bentolila
  • Michael L Hayes
  • Christine P Halter
  • Thomas G Owens
  • Anne Laure Chateigner-Boutin

Detail Information

Publications3

  1. pmc Expression of complementary RNA from chloroplast transgenes affects editing efficiency of transgene and endogenous chloroplast transcripts
    Carla E Hegeman
    Department of Molecular Biology and Genetics, Cornell University Ithaca, NY 14853, USA
    Nucleic Acids Res 33:1454-64. 2005
    ..Chloroplast transgenic plants expressing the three different antisense RNA constructs exhibited abnormal growth and development, though plants expressing the 92 nt sense transcripts were phenotypically normal...
  2. ncbi Substrate and cofactor requirements for RNA editing of chloroplast transcripts in Arabidopsis in vitro
    Carla E Hegeman
    Department of Molecular Biology and Genetics, Cornell University, Biotechnology Building, Ithaca, NY 14853, USA
    Plant J 42:124-32. 2005
    ..Editing was sensitive to a zinc chelator, also a characteristic of the mammalian APOBEC editing enzyme, which is a zinc-dependent cytidine deaminase...
  3. pmc Ecotype allelic variation in C-to-U editing extent of a mitochondrial transcript identifies RNA-editing quantitative trait loci in Arabidopsis
    Stephane Bentolila
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Plant Physiol 139:2006-16. 2005
    ..A member of the pentatricopeptide repeat protein family that carries a putative mitochondrial transit sequence has been identified near a major quantitative trait locus on chromosome 4...