Mechanism of aPL antibody-induced Pregnancy loss

Summary

Principal Investigator: J E Salmon
Abstract: DESCRIPTION (provided by applicant): The antiphospholipid syndrome (APS) is characterized by thrombosis and pregnancy loss that occur in the presence of antiphospholipid (aPL) antibodies. Pregnancy complications in women with APS include fetal death, preeclampsia, and intrauterine growth restriction (IUGR). The pathogenic mechanisms that lead to such injury are incompletely understood and therapy for pregnant women with APS is only partially successful. Our studies in a murine model of APS induced by passive transfer of human aPL antibodies indicate that complement activation plays an essential and causative role in fetal loss and growth restriction. In addition, treatment with heparin, the standard therapy for pregnant patients with APS, prevents complement activation and protects mice from pregnancy complications induced by aPL antibodies, while anticoagulants that do not inhibit complement do not protect pregnancies. These studies indicate that APS is an inflammatory disease and suggest that complement inhibitory therapy might be an effective treatment. Our overall goals are to use the murine model of APS to determine how complement is activated, which complement products mediate the clinical complications associated with aPL antibodies, and the relative role of complement activation within the overall inflammatory cascade. In addition, we propose to test the hypothesis that activation of complement at the maternal-fetal interface plays an etiologic role in IUGR. Accordingly, our aims are: Aim 1. To determine which complement components and receptors are necessary or sufficient to mediate aPL antibodies-induced placental injury, fetal loss and/or IUGR. (a) To identify the pathways that initiate complement activation and lead(s) to poor pregnancy outcomes;(b) To identify the complement activation products and receptors that mediate fetal injury;(c) To assess the role of murine complement regulatory proteins in the control of local complement activation. Aim 2. To define the role of aPL antibody-mediated complement activation in the overall inflammatory cascade to identify complement- dependent vs. complement-independent mechanisms, (a) To define the contribution of Fc-gammaR to aPL antibody mediated injury;(b) To define the cellular and cytokine mediators which contribute to IUGR and fetal loss. RELEVANCE: The antiphospholipid syndrome (APS) is a leading cause of miscarriage and maternal and fetal morbidity. The proposed study, together with our ongoing work to define the role of complement in pregnancy complications in APS patients, will provide insights into the mechanisms by which complement induces disease and define targets for interventions to prevent aPL antibody-associated fetal demise and IUGR. Additionally, understanding how aPL antibodies "cause" pregnancy loss may translate into new concepts about maternal-fetal tolerance and miscarriages in general and benefit women with non-APS- related pregnancy complications.
Funding Period: ----------------1992 - ---------------2011-
more information: NIH RePORT

Top Publications

  1. pmc Antiphospholipid antibodies and pregnancy loss: a disorder of inflammation
    Jane E Salmon
    Autoimmunity and Inflammation Program, Hospital for Special Surgery, Department of Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Reprod Immunol 77:51-6. 2008
  2. pmc Pathogenic role of antiphospholipid antibodies
    J E Salmon
    Department of Medicine, Hospital for Special Surgery, Weill Medical College of Cornell University, New York, New York 10021, USA
    Lupus 17:405-11. 2008
  3. pmc Engraftment of peripheral blood mononuclear cells from systemic lupus erythematosus and antiphospholipid syndrome patient donors into BALB-RAG-2-/- IL-2Rγ-/- mice: a promising model for studying human disease
    Danieli Andrade
    Hospital for Special Surgery, New York, New York 10021, USA
    Arthritis Rheum 63:2764-73. 2011
  4. pmc Complement regulates conventional DC-mediated NK-cell activation by inducing TGF-β1 in Gr-1+ myeloid cells
    Xiaoping Qing
    Program in Inflammation and Autoimmunity, Hospital for Special Surgery, New York, NY, USA
    Eur J Immunol 42:1723-34. 2012

Detail Information

Publications4

  1. pmc Antiphospholipid antibodies and pregnancy loss: a disorder of inflammation
    Jane E Salmon
    Autoimmunity and Inflammation Program, Hospital for Special Surgery, Department of Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Reprod Immunol 77:51-6. 2008
    ..These studies underscore the importance of inflammation in fetal injury associated with aPL antibodies and raise the importance of developing and testing targeted complement inhibitory therapy for patients with APS...
  2. pmc Pathogenic role of antiphospholipid antibodies
    J E Salmon
    Department of Medicine, Hospital for Special Surgery, Weill Medical College of Cornell University, New York, New York 10021, USA
    Lupus 17:405-11. 2008
    ..We will discuss the current knowledge on how aPL antibodies trigger increased inflammation and enhanced thrombotic tendency, and thereby lead to tissue damage...
  3. pmc Engraftment of peripheral blood mononuclear cells from systemic lupus erythematosus and antiphospholipid syndrome patient donors into BALB-RAG-2-/- IL-2Rγ-/- mice: a promising model for studying human disease
    Danieli Andrade
    Hospital for Special Surgery, New York, New York 10021, USA
    Arthritis Rheum 63:2764-73. 2011
    ..To construct a humanized mouse model of systemic lupus erythematosus (SLE) that resembles the human disease in order to define the pathophysiology and targets for treatments...
  4. pmc Complement regulates conventional DC-mediated NK-cell activation by inducing TGF-β1 in Gr-1+ myeloid cells
    Xiaoping Qing
    Program in Inflammation and Autoimmunity, Hospital for Special Surgery, New York, NY, USA
    Eur J Immunol 42:1723-34. 2012
    ..Thus, complement activation indirectly regulates cDC-NK-cell activation in response to inflammatory stimuli such as TLR3 by promoting TGF-β1 production in Gr-1(+) myeloid cells at steady state...