A Combinatorial Approach to Wound Healing for Protein, Gene and Cell Therapeutics

Summary

Principal Investigator: Andrew Baird
Abstract: DESCRIPTION (provided by applicant): We propose an 14 month, milestone-driven, benchmarked research plan that extends a parent P20 Center grant focusing on bio-therapeutics development. The research will initially be executed by 6 newly hired academic research and technical positions at UCSD. Sustained and new employment in drug discovery, drug screening and drug development will be developed by partnerships with the San Diego's local biotechnology community. Specifically we will: (1) express a newly discovered recombinant neuropeptide protein that we identified in the parent grant (P20 GM078421). Analogs will be synthesized to identify functional domains and test agonists and antagonists. (2) develop high specificity monoclonal antibodies and create a quantitative ELISA assay to measure its role in disease. (3) evaluate preclinical models of wound repair by creating models of gene knock down and conditional knock outs to (a) establish its physiological relevance, (b) serve as models for drug discovery and (c) assist in converting the drug candidate to a drug lead. (4) identify its receptor by molecular cloning to establish an assay for drug discovery. (5) map the promoter to create a luciferase reporter transgenic mouse. (6) transfer technology generated to the private sector for further development. Funding will build on the success of the parent project, extend its scope, bring new fundamental skills to the laboratory, accelerate the tempo of scientific research and allow for significant job creation (and retention) by enabling hiring of additional staff, increasing hours of current part-time staff, and contracting for additional needed skills. The funded program will create 6 new full time positions, enable us to sustain 2 ongoing current research positions and with a successful outcome, we anticipate would generate an additional 5-15 new jobs in drug development research positions by technology transfer to the private biotechnology sector. Relevance: This research project describes 5 milestone and bench-marked specific AIMs that will create, test and validate a new therapeutic for wound healing. It will immediately create 6 full time positions and, if successful, its 6th AIM will create 5-15 new jobs by technology transfer to the private sector.
Funding Period: 2009-09-30 - 2010-07-31
more information: NIH RePORT

Top Publications

  1. pmc The candidate tumor suppressor gene Ecrg4 as a wound terminating factor in cutaneous injury
    Ashkaun Shaterian
    Division of Trauma, Surgical Critical Care and Burns, Department of Surgery, University of California San Diego School of Medicine, 212 Dickinson Street, San Diego, CA 92103, USA
    Arch Dermatol Res 305:141-9. 2013
  2. pmc Traumatic brain injury and recovery mechanisms: peptide modulation of periventricular neurogenic regions by the choroid plexus-CSF nexus
    Conrad Johanson
    Department of Neurosurgery, Warren Alpert Medical School at Brown University, Providence, RI 02903, USA
    J Neural Transm 118:115-33. 2011
  3. pmc Zebrafish model of the blood-brain barrier: morphological and permeability studies
    Brian P Eliceiri
    Department of Surgery, University of California San Diego, San Diego, CA, USA
    Methods Mol Biol 686:371-8. 2011
  4. pmc Targeting the choroid plexus-CSF-brain nexus using peptides identified by phage display
    Andrew Baird
    Department of Surgery, Division of Trauma, Burns and Critical Care, University of California San Diego, San Diego, CA, USA
    Methods Mol Biol 686:483-98. 2011
  5. pmc Conditional deletion of the focal adhesion kinase FAK alters remodeling of the blood-brain barrier in glioma
    Jisook Lee
    Department of Surgery, University of California San Diego, California 92103 8236, USA
    Cancer Res 70:10131-40. 2010
  6. pmc Non-invasive quantification of brain tumor-induced astrogliosis
    Jisook Lee
    Department of Surgery, University of California San Diego, San Diego, CA, USA
    BMC Neurosci 12:9. 2011
  7. pmc A phage-targeting strategy for the design of spatiotemporal drug delivery from grafted matrices
    Ritsuko Sawada
    Department of Surgery, Division of Trauma, Surgical Critical Care and Burns, University of California San Diego School of Medicine, 200 W, Arbor Dr, San Diego, CA 92103 8236 USA
    Fibrogenesis Tissue Repair 4:7. 2011
  8. pmc Burn-induced acute lung injury requires a functional Toll-like receptor 4
    Michael Krzyzaniak
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California, San Diego School of Medicine, San Diego, California, USA
    Shock 36:24-9. 2011
  9. pmc Ecrg4 expression and its product augurin in the choroid plexus: impact on fetal brain development, cerebrospinal fluid homeostasis and neuroprogenitor cell response to CNS injury
    Ana Maria Gonzalez
    College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Fluids Barriers CNS 8:6. 2011
  10. pmc Postinjury vagal nerve stimulation protects against intestinal epithelial barrier breakdown
    Michael Krzyzaniak
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California, San Diego School of Medicine, San Diego, California 92103 8896, USA
    J Trauma 70:1168-75; discussion 1175-6. 2011

Scientific Experts

  • Andrew Baird
  • Ana M Gonzalez
  • Vishal Bansal
  • Brian P Eliceiri
  • Raul Coimbra
  • Todd W Costantini
  • Carrie Y Peterson
  • Paul Wolf
  • James G Putnam
  • Michael Krzyzaniak
  • William H Loomis
  • Jisook Lee
  • Brian Eliceiri
  • Ashkaun Shaterian
  • Xitong Dang
  • Nicole E Lopez
  • William Loomis
  • Ritsuko Sawada
  • Arwa Kurabi
  • Michael J Krzyzaniak
  • Sonia Podvin
  • James Putnam
  • Gerald Cheadle
  • Hyun Bae Chun
  • Alexandra K Borboa
  • Alexandra Borboa
  • Lauren Kroll
  • Yan T Ortiz-Pomales
  • Yan Ortiz-Pomales
  • Ann Marie Hageny
  • Carrie Peterson
  • Conrad Johanson
  • Bruce M Potenza
  • Sunghyuk Choi
  • Amy H Lin
  • Jessica Deree
  • Allen F Ryan
  • Lin Chen
  • Luisa A DiPietro
  • Kwang Pak
  • Steven Kao
  • Chelsea Blow
  • Nicole Lopez
  • Luiz Guilherme Reys
  • Antonio De Maio
  • Heather Hoover
  • Matthew Boissaud-Cooke
  • Vince Kennedy
  • Conrad E Johanson
  • Edward Stopa
  • Hannah Botfield
  • Michael Scott
  • Bruce E Torbett
  • Edward G Stopa
  • Sherry Niessen
  • Miles C Miller
  • Wendy E Leadbeater
  • Barbara Mueller
  • Hari Sharma
  • John Yates
  • Luiz Reys
  • Ryan Rossi
  • John E Donahue
  • Fermin Rankin
  • Todd Costantini
  • Bruce Potenza
  • Dana Drusinsky
  • Caren Lund-Smith
  • Eugene G Levin

Detail Information

Publications45

  1. pmc The candidate tumor suppressor gene Ecrg4 as a wound terminating factor in cutaneous injury
    Ashkaun Shaterian
    Division of Trauma, Surgical Critical Care and Burns, Department of Surgery, University of California San Diego School of Medicine, 212 Dickinson Street, San Diego, CA 92103, USA
    Arch Dermatol Res 305:141-9. 2013
    ..These findings point to the candidate tumor suppressor gene Ecrg4 as a novel, biologically active, constituent of skin and skin injury. The possibility that Ecrg4 serves as a wound termination factor during wound resolution is discussed...
  2. pmc Traumatic brain injury and recovery mechanisms: peptide modulation of periventricular neurogenic regions by the choroid plexus-CSF nexus
    Conrad Johanson
    Department of Neurosurgery, Warren Alpert Medical School at Brown University, Providence, RI 02903, USA
    J Neural Transm 118:115-33. 2011
    ..CSF translational research presents opportunities that involve CP and ependymal manipulations to expedite recovery from TBI...
  3. pmc Zebrafish model of the blood-brain barrier: morphological and permeability studies
    Brian P Eliceiri
    Department of Surgery, University of California San Diego, San Diego, CA, USA
    Methods Mol Biol 686:371-8. 2011
    ....
  4. pmc Targeting the choroid plexus-CSF-brain nexus using peptides identified by phage display
    Andrew Baird
    Department of Surgery, Division of Trauma, Burns and Critical Care, University of California San Diego, San Diego, CA, USA
    Methods Mol Biol 686:483-98. 2011
    ..This chapter describes three selection strategies, which can be used to identify peptides that target the choroid plexus (CP) directly or for drug translocation across the CP and into cerebrospinal fluid...
  5. pmc Conditional deletion of the focal adhesion kinase FAK alters remodeling of the blood-brain barrier in glioma
    Jisook Lee
    Department of Surgery, University of California San Diego, California 92103 8236, USA
    Cancer Res 70:10131-40. 2010
    ..Our findings may help understand how therapeutics might be used to regulate specific cell-type interactions to restore BBB structure/function in cancer and perhaps other pathologic conditions...
  6. pmc Non-invasive quantification of brain tumor-induced astrogliosis
    Jisook Lee
    Department of Surgery, University of California San Diego, San Diego, CA, USA
    BMC Neurosci 12:9. 2011
    ..However, the kinetics of astrogliosis that is related to these insults (i.e. tumor) is largely unknown...
  7. pmc A phage-targeting strategy for the design of spatiotemporal drug delivery from grafted matrices
    Ritsuko Sawada
    Department of Surgery, Division of Trauma, Surgical Critical Care and Burns, University of California San Diego School of Medicine, 200 W, Arbor Dr, San Diego, CA 92103 8236 USA
    Fibrogenesis Tissue Repair 4:7. 2011
    ..abstract:..
  8. pmc Burn-induced acute lung injury requires a functional Toll-like receptor 4
    Michael Krzyzaniak
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California, San Diego School of Medicine, San Diego, California, USA
    Shock 36:24-9. 2011
    ..Toll-like receptor 4 KO animals were clearly protected and had a much less severe lung injury. Our data suggest that burn-induced ALI is a TLR4-dependent process...
  9. pmc Ecrg4 expression and its product augurin in the choroid plexus: impact on fetal brain development, cerebrospinal fluid homeostasis and neuroprogenitor cell response to CNS injury
    Ana Maria Gonzalez
    College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Fluids Barriers CNS 8:6. 2011
    ..abstract:..
  10. pmc Postinjury vagal nerve stimulation protects against intestinal epithelial barrier breakdown
    Michael Krzyzaniak
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California, San Diego School of Medicine, San Diego, California 92103 8896, USA
    J Trauma 70:1168-75; discussion 1175-6. 2011
    ..Therefore, we postulated that VNS conducted postinjury would also have a similar protective effect on maintaining gut epithelial barrier integrity...
  11. pmc Non-invasive detection of spatio-temporal activation of SBE and NFAT5 promoters in transgenic reporter mice following stroke
    Ashkaun Shaterian
    Department of Surgery, University of California of San Diego, San Diego, California 92121, USA
    Neuropathology 32:118-23. 2012
    ..e., radiance in photons/sec/cm(2) /steradian) as well as qualitative localization of the molecular response following focal ischemic injury...
  12. pmc Efferent vagal nerve stimulation attenuates acute lung injury following burn: The importance of the gut-lung axis
    Michael J Krzyzaniak
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California, San Diego School of Medicine, San Diego, CA, USA
    Surgery 150:379-89. 2011
    ..The purpose of this study was to assess acute lung injury when protection to the gut mucosal barrier offered by vagus nerve stimulation is eliminated by an abdominal vagotomy...
  13. pmc The proteome of mouse brain microvessel membranes and basal lamina
    Hyun Bae Chun
    Department of Surgery, School of Medicine, University of California San Diego, San Diego, California 92103, USA
    J Cereb Blood Flow Metab 31:2267-81. 2011
    ....
  14. pmc In vivo measurement of glioma-induced vascular permeability
    Jisook Lee
    Department of Surgery, University of California San Diego, San Diego, CA, USA
    Methods Mol Biol 763:417-22. 2011
    ..Herein, we describe methods to quantitatively measure glioma-induced vascular permeability, utilizing an orthotopic xenograft model of glioma...
  15. pmc Vagal nerve stimulation blocks peritoneal macrophage inflammatory responsiveness after severe burn injury
    Nicole E Lopez
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego Health Sciences, San Diego, California
    Shock 38:294-300. 2012
    ..In combination, these studies suggest VNS mediates the inflammatory response in peritoneal macrophages by affecting the set point of LPS responsiveness...
  16. pmc Vagus nerve stimulation blocks vascular permeability following burn in both local and distal sites
    Yan T Ortiz-Pomales
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego Health Sciences, MC 8236, 212 Dickinson Street, San Diego, CA 92103, USA
    Burns 39:68-75. 2013
    ..These studies identify a role for VNS in the regulation of VP in burns, with the translational potential of attenuating lung complications following burn...
  17. pmc The hormone ghrelin prevents traumatic brain injury induced intestinal dysfunction
    Vishal Bansal
    Department of Surgery, Division of Trauma, Surgical Critical Care and Burns, University of California San Diego, San Diego, California 92103, USA
    J Neurotrauma 27:2255-60. 2010
    ..The mechanism for ghrelin mediating intestinal protection is likely multifactorial, and further studies are needed to delineate these possibilities...
  18. pmc Role of p38 MAPK in burn-induced intestinal barrier breakdown
    Todd W Costantini
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego School of Medicine, San Diego, CA 92103 8896, USA
    J Surg Res 156:64-9. 2009
    ....
  19. pmc FAK mediates the inhibition of glioma cell migration by truncated 24 kDa FGF-2
    Amy H Lin
    Torrey Pines Institute for Molecular Studies, 3550 General Atomics Court, San Diego, CA 92121, United States
    Biochem Biophys Res Commun 382:503-7. 2009
    ..These data suggest that the inhibition of cell migration by ATE+31 occurs via Tyr(407)-FAK and Ser(732)-FAK...
  20. pmc Traumatic brain injury and intestinal dysfunction: uncovering the neuro-enteric axis
    Vishal Bansal
    Department of Surgery, Division of Trauma, Surgical Critical Care and Burns, University of California San Diego, San Diego, CA 92103, USA
    J Neurotrauma 26:1353-9. 2009
    ..Expression of intestinal tight junction proteins may be an important factor in gastrointestinal dysfunction following brain injury...
  21. pmc Ecrg4 attenuates the inflammatory proliferative response of mucosal epithelial cells to infection
    Arwa Kurabi
    Department of Surgery, University of California, San Diego School of Medicine, La Jolla, California, United States of America
    PLoS ONE 8:e61394. 2013
    ..Taken together, these data support a hypothesis that Ecrg4 plays a role in coordinating the inflammatory and proliferative response to infection of mucosal epithelium suggesting a possible mechanism for its putative anti-tumor activity...
  22. pmc The deployment of adenovirus-containing gene activated matrices onto severed axons after central nervous system injury leads to transgene expression in target neuronal cell bodies
    Ana Maria Gonzalez
    Molecular Neuroscience Group, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    J Gene Med 11:679-88. 2009
    ..Lyophilized GAMs containing collagen and adenoviral vectors were assessed in vitro and in vivo...
  23. pmc Glioma-induced remodeling of the neurovascular unit
    Jisook Lee
    Department of Surgery, Division of Trauma, 212 Dickinson Street, San Diego, CA 92103, USA
    Brain Res 1288:125-34. 2009
    ..We suggest that these changes may be essential to understand the capacity of gliomas to regulate BBB integrity and as such, glioma invasion into brain parenchyma...
  24. pmc Pentoxifylline modulates intestinal tight junction signaling after burn injury: effects on myosin light chain kinase
    Todd W Costantini
    Department of Surgery, Division of Trauma, Surgical Critical Care, and Burns, University of California, San Diego School of Medicine, San Diego, California 92103 8896, USA
    J Trauma 66:17-24; discussion 24-5. 2009
    ..Therefore, we postulate that PTX will attenuate activation of the tight junction protein MLCK, which may decrease intestinal tight junction permeability after severe burn...
  25. pmc Targeting the gut barrier: identification of a homing peptide sequence for delivery into the injured intestinal epithelial cell
    Todd W Costantini
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego School of Medicine, San Diego, CA 92103 8896, USA
    Surgery 146:206-12. 2009
    ..We postulated that mining a peptide library that was displayed on phage would identify peptide sequences that bind and internalize into the gut epithelium following injury...
  26. ncbi Phosphodiesterase inhibition attenuates alterations to the tight junction proteins occludin and ZO-1 in immunostimulated Caco-2 intestinal monolayers
    Todd W Costantini
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego School of Medicine, San Diego, California, United States
    Life Sci 84:18-22. 2009
    ..We hypothesize that PTX prevents the breakdown of ZO-1 and occludin in an in vitro model of immunostimulated intestinal cell monolayers...
  27. pmc Burns, inflammation, and intestinal injury: protective effects of an anti-inflammatory resuscitation strategy
    Todd W Costantini
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego School of Medicine, San Diego, California, USA
    J Trauma 67:1162-8. 2009
    ..We hypothesized that PTX would limit intestinal barrier breakdown and attenuate inflammatory signaling via the MAPK pathway...
  28. pmc Co-localization and regulation of basic fibroblast growth factor and arginine vasopressin in neuroendocrine cells of the rat and human brain
    Ana M Gonzalez
    School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK
    Cerebrospinal Fluid Res 7:13. 2010
    ..To ascertain a functional link between arginine vasopressin (AVP) and FGF2, a rat model of chronic dehydration was used to test the hypothesis that FGF2 expression, like that of AVP, is altered by perturbed fluid balance...
  29. pmc Vagal nerve stimulation protects against burn-induced intestinal injury through activation of enteric glia cells
    Todd W Costantini
    Department of Surgery, University of California, San Diego School of Medicine, USA
    Am J Physiol Gastrointest Liver Physiol 299:G1308-18. 2010
    ..The vagus nerve may mediate the signaling that occurs from the central nervous system to the enteric nervous system following gastrointestinal injury...
  30. pmc Efferent vagal nerve stimulation attenuates gut barrier injury after burn: modulation of intestinal occludin expression
    Todd W Costantini
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego School of Medicine, San Diego, California, USA
    J Trauma 68:1349-54; discussion 1354-6. 2010
    ....
  31. pmc Stimulating the central nervous system to prevent intestinal dysfunction after traumatic brain injury
    Vishal Bansal
    Department of Surgery, Division of Trauma, Surgical Critical Care and Burns, University of California San Diego, California, USA
    J Trauma 68:1059-64. 2010
    ..Regulation of the gut barrier may involve the central nervous system. We hypothesize that vagal nerve stimulation prevents an increase in intestinal permeability after TBI...
  32. pmc Toll-like receptor-4 mediates intestinal barrier breakdown after thermal injury
    Carrie Y Peterson
    Division of Trauma, Critical Care and Burns, Department of Surgery, University of California, San Diego, San Diego, California 92103 8896, USA
    Surg Infect (Larchmt) 11:137-44. 2010
    ..We hypothesized that increased intestinal permeability and barrier breakdown after burns is a TLR-4 dependent process; hence, knocking down the TLR-4 gene would have a protective effect on burn-induced intestinal dysfunction...
  33. pmc Burn-induced gut barrier injury is attenuated by phosphodiesterase inhibition: effects on tight junction structural proteins
    Todd W Costantini
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego School of Medicine, San Diego, California, USA
    Shock 31:416-22. 2009
    ..Therefore, phosphodiesterase inhibition may be a useful adjunct strategy in the attenuation of burn-induced gut barrier injury...
  34. pmc Quantitative assessment of intestinal injury using a novel in vivo, near-infrared imaging technique
    Todd W Costantini
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego School of Medicine, San Diego, CA, USA
    Mol Imaging 9:30-9. 2010
    ..Our results correlate with traditional, time-consuming, semiquantitative assays of intestinal injury, now allowing the noninvasive, nonlethal assessment of injury over time...
  35. pmc Cell-specific processing and release of the hormone-like precursor and candidate tumor suppressor gene product, Ecrg4
    Xitong Dang
    Department of Surgery, School of Medicine University of California, San Diego, San Diego, CA 92103, USA
    Cell Tissue Res 348:505-14. 2012
    ....
  36. pmc Real-time analysis of the kinetics of angiogenesis and vascular permeability in an animal model of wound healing
    Ashkaun Shaterian
    Department of Surgery, University of California, San Diego, CA 92103, USA
    Burns 35:811-7. 2009
    ..We propose a non-invasive strategy to assess VP of the wound capillary bed will facilitate a better understanding of the cell and molecular basis of angiogenesis in wound healing...
  37. pmc CPSI-121 pharmacologically prevents intestinal barrier dysfunction after cutaneous burn through a vagus nerve-dependent mechanism
    Michael Krzyzaniak
    Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California San Diego School of Medicine, San Diego, CA 92103, USA
    J Trauma Acute Care Surg 72:355-61; discussion 361-3. 2012
    ....
  38. pmc Ghrelin prevents disruption of the blood-brain barrier after traumatic brain injury
    Nicole E Lopez
    Department of Surgery, University of California San Diego, San Diego, California, USA
    J Neurotrauma 29:385-93. 2012
    ..This is evident by a decrease in vascular permeability that is linked to a decrease in AQP-4. This decrease in vascular permeability may diminish post-TBI brain tissue damage was evident by decreased S100B...
  39. pmc Esophageal cancer related gene-4 is a choroid plexus-derived injury response gene: evidence for a biphasic response in early and late brain injury
    Sonia Podvin
    Department of Surgery, School of Medicine, University of California San Diego, San Diego, California, United States of America
    PLoS ONE 6:e24609. 2011
    ..Accordingly, augurin would play a constitutive inhibitory function in normal CNS while down regulation of Ecrg4 gene expression in injury, like in cancer, dysinhibits proliferation...
  40. pmc Epidermal growth factor targeting of bacteriophage to the choroid plexus for gene delivery to the central nervous system via cerebrospinal fluid
    Ana Maria Gonzalez
    School of Clinical and Experimental Medicine, University of Birmingham, Edgbaston, UK
    Brain Res 1359:1-13. 2010
    ....
  41. pmc Vagal stimulation modulates inflammation through a ghrelin mediated mechanism in traumatic brain injury
    Vishal Bansal
    Department of Surgery, Division of Trauma, Surgical Critical Care and Burns, University of California San Diego, 200 W Arbor Drive 8896, San Diego, CA 92103, USA
    Inflammation 35:214-20. 2012
    ..In conclusion, VNS increased serum ghrelin and decreased TNF-α following TBI. This was abrogated with GRa. Our data suggests that ghrelin plays an important role in the anti-inflammatory effects of VNS following TBI...
  42. pmc The noninvasive, quantitative, in vivo assessment of adenoviral-mediated gene delivery in skin wound biomaterials
    Carrie Y Peterson
    Division of Trauma, Burns and Critical Care, Department of Surgery, 200 W Arbor Dr, University of California San Diego, San Diego, CA 92103 8236, USA
    Biomaterials 30:6788-93. 2009
    ..Taken together, these results show how a noninvasive and quantitative assessment of gene expression can be used to optimize gene delivery and that the composition of matrices can dramatically influence gene expression in the wound bed...
  43. pmc Targeting choroid plexus epithelia and ventricular ependyma for drug delivery to the central nervous system
    Ana Maria Gonzalez
    School of Experimental Medicine and Dentistry, University of Birmingham, Edgbaston, UK
    BMC Neurosci 12:4. 2011
    ..To this end, we sought to identify peptides capable of ligand-mediated targeting to CP epithelial cells reasoning that they could be exploited to deliver drugs, biotherapeutics and genes to the CNS...
  44. pmc Cell surface localization and release of the candidate tumor suppressor Ecrg4 from polymorphonuclear cells and monocytes activate macrophages
    Andrew Baird
    University of California San Diego School of Medicine, 212 Dickinson St, MC 8236, San Diego, CA 92103, USA
    J Leukoc Biol 91:773-81. 2012
    ....