p2rx4a

Summary

Gene Symbol: p2rx4a
Description: purinergic receptor P2X, ligand-gated ion channel, 4a
Alias: p2rx4, p2x4, wu:fi03f02, P2X purinoceptor 4, etID14865.21, zP2X4.1
Species: zebrafish

Top Publications

  1. pmc Crystal structure of the ATP-gated P2X(4) ion channel in the closed state
    Toshimitsu Kawate
    Vollum Institute, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Oregon 97239, USA
    Nature 460:592-8. 2009
  2. pmc Pore architecture and ion sites in acid-sensing ion channels and P2X receptors
    Eric B Gonzales
    Vollum Institute, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nature 460:599-604. 2009
  3. pmc Molecular mechanism of ATP binding and ion channel activation in P2X receptors
    Motoyuki Hattori
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nature 485:207-12. 2012
  4. ncbi Cloning and characterization of two novel zebrafish P2X receptor subunits
    Miguel Diaz-Hernandez
    Department of Pharmacological and Physiological Sciences, Saint Louis University School of Medicine, 1402 S Grand Blvd, St Louis, MO 63104, USA
    Biochem Biophys Res Commun 295:849-53. 2002
  5. pmc Conductance of P2X4 purinergic receptor is determined by conformational equilibrium in the transmembrane region
    Yuichi Minato
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 113:4741-6. 2016
  6. doi Geometric rules of channel gating inferred from computational models of the P2X receptor transmembrane domain
    Guo Hua Li
    School of Life Sciences, Changchun Normal University, Changchun 130032, China Electronic address
    J Mol Graph Model 61:107-14. 2015
  7. pmc Amino acid variations resulting in functional and nonfunctional zebrafish P2X(1) and P2X (5.1) receptors
    Sean E Low
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 N University Ave, Ann Arbor, MI, 48109 1048, USA
    Purinergic Signal 4:383-92. 2008
  8. doi Solution structure of the rat P2X4 receptor head domain involved in inhibitory metal binding
    Tatsuhiro Igawa
    Department of Protein Structure, Function and Design, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    FEBS Lett 589:680-6. 2015
  9. doi Identification and characterization of a selective allosteric antagonist of human P2X4 receptor channels
    Ariel R Ase
    Montreal Neurological Institute, Alan Edwards Centre for Research on Pain, Department of Neurology and Neurosurgery, McGill University, Montreal, Canada A R A, P S Screening Division, The Centre for Drug Research and Discovery, Vancouver, Canada N S H, T A P and Zamboni Chemical Solutions, Department of Chemistry, McGill University, Montreal, Canada H Z
    Mol Pharmacol 87:606-16. 2015
  10. doi Purinergic P2X receptors: structural models and analysis of ligand-target interaction
    Diego Dal Ben
    School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S Agostino 1, 62032 Camerino, MC, Italy Electronic address
    Eur J Med Chem 89:561-80. 2015

Scientific Experts

  • Diego Dal Ben
  • Guo Hua Li
  • Ralf Hausmann
  • Toshimitsu Kawate
  • Motoyuki Hattori
  • Vendula Tvrdonova
  • Eric Gouaux
  • Yuichi Minato
  • Tatsuhiro Igawa
  • Ariel R Ase
  • Li Dong Huang
  • Yanling Jie
  • Ji Xu
  • Gabriel Heymann
  • Huan Xiang Zhou
  • Juan Du
  • Mufeng Li
  • Sukanya Punthambaker
  • Shai D Silberberg
  • Kenton J Swartz
  • Maria Valente
  • Nataliia Petrenko
  • Richard I Hume
  • Mark T Young
  • Eric B Gonzales
  • Lishuang Cao
  • Terrance M Egan
  • Sean E Low
  • Lior Appelbaum
  • Ichio Shimada
  • Tomoaki Hara
  • Takumi Ueda
  • Yutaka Kofuku
  • S Kucenas
  • Go Kasuya
  • Shunsuke Igarashi
  • Yuichiro Fujiwara
  • Shiho Suzuki
  • Ei ichiro Suzuki
  • Osamu Nureki
  • Tom A Pfeifer
  • Tadashi Ueda
  • Helmi Zaghdane
  • Nicolette S Honson
  • Makoto Tsuda
  • Yoshito Abe
  • Kazuhide Inoue
  • Philippe Séguéla
  • Xiao Yang Cheng
  • Hua Chai
  • Rahul Srinivasan
  • Baljit S Khakh
  • Longmei Zhang
  • Konstantin Ehinger
  • Yang Yang
  • Xiang Yang Lu
  • Peng Cao
  • Huijuan Xu
  • Wenbo Ma
  • Wen Chao Zhou
  • Miguel Diaz-Hernandez
  • Chao Gao
  • Wen Shan Zhao
  • Ye Yu
  • Milos B Rokic
  • Hana Zemkova
  • Yan Liu
  • Zhiyuan Li
  • Stanko S Stojilkovic
  • Jin Wang
  • Ying zhe Fan
  • Yun Tian
  • Manfred Frick
  • Jian Dai
  • Hao Dong
  • Jacob A Blum
  • Rashid Giniatullin
  • Kamil Khafizov
  • Mark D Parker
  • Summer J Watterson
  • Andrei Skorinkin
  • Janice L Robertson
  • Robert C Ford
  • Jennifer Carlisle Michel
  • R Alan North
  • William T Birdsong
  • Helen E Broomhead
  • John Y Kuwada
  • Emmanuel Mignot
  • Philippe Mourrain

Detail Information

Publications24

  1. pmc Crystal structure of the ATP-gated P2X(4) ion channel in the closed state
    Toshimitsu Kawate
    Vollum Institute, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Oregon 97239, USA
    Nature 460:592-8. 2009
    ..We define the location of three non-canonical, intersubunit ATP-binding sites, and suggest that ATP binding promotes subunit rearrangement and ion channel opening...
  2. pmc Pore architecture and ion sites in acid-sensing ion channels and P2X receptors
    Eric B Gonzales
    Vollum Institute, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nature 460:599-604. 2009
    ....
  3. pmc Molecular mechanism of ATP binding and ion channel activation in P2X receptors
    Motoyuki Hattori
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nature 485:207-12. 2012
    ..Here we report the crystal structure of the zebrafish P2X4 receptor in complex with ATP and a new structure of the apo receptor...
  4. ncbi Cloning and characterization of two novel zebrafish P2X receptor subunits
    Miguel Diaz-Hernandez
    Department of Pharmacological and Physiological Sciences, Saint Louis University School of Medicine, 1402 S Grand Blvd, St Louis, MO 63104, USA
    Biochem Biophys Res Commun 295:849-53. 2002
    ..Both subunit genes were physically mapped onto the zebrafish genome using radiation hybrid analysis of the T51 panel, with the zp2x4 localized to LG21 and zp2x5 to LG5...
  5. pmc Conductance of P2X4 purinergic receptor is determined by conformational equilibrium in the transmembrane region
    Yuichi Minato
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 113:4741-6. 2016
    ..underlying the partial activation of the P2X receptors is unknown although the crystal structures of zebrafish P2X4 receptor in the apo and ATP-bound states are available...
  6. doi Geometric rules of channel gating inferred from computational models of the P2X receptor transmembrane domain
    Guo Hua Li
    School of Life Sciences, Changchun Normal University, Changchun 130032, China Electronic address
    J Mol Graph Model 61:107-14. 2015
    ..Here, I have reconstructed the three-dimensional models of the zebrafish P2X4 transmembrane domain using spatial restraints on helical packing...
  7. pmc Amino acid variations resulting in functional and nonfunctional zebrafish P2X(1) and P2X (5.1) receptors
    Sean E Low
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 N University Ave, Ann Arbor, MI, 48109 1048, USA
    Purinergic Signal 4:383-92. 2008
    ..1). Antisense knockdown of P2X(5.1) eliminated skeletal muscle responsiveness to ATP but did not prevent myogenesis or behaviors that require functional transmission at the neuromuscular junction...
  8. doi Solution structure of the rat P2X4 receptor head domain involved in inhibitory metal binding
    Tatsuhiro Igawa
    Department of Protein Structure, Function and Design, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    FEBS Lett 589:680-6. 2015
    ..The head domain (Gln111-Val167 in the rat P2X4 receptor) regulates ATP-induced cation influx...
  9. doi Identification and characterization of a selective allosteric antagonist of human P2X4 receptor channels
    Ariel R Ase
    Montreal Neurological Institute, Alan Edwards Centre for Research on Pain, Department of Neurology and Neurosurgery, McGill University, Montreal, Canada A R A, P S Screening Division, The Centre for Drug Research and Discovery, Vancouver, Canada N S H, T A P and Zamboni Chemical Solutions, Department of Chemistry, McGill University, Montreal, Canada H Z
    Mol Pharmacol 87:606-16. 2015
    b>P2X4 is an ATP-gated nonselective cation channel highly permeable to calcium...
  10. doi Purinergic P2X receptors: structural models and analysis of ligand-target interaction
    Diego Dal Ben
    School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S Agostino 1, 62032 Camerino, MC, Italy Electronic address
    Eur J Med Chem 89:561-80. 2015
    ..The publication of the crystal structures of zebrafish P2X4 receptor in inactive and ATP-bound active forms provided structural data for the analysis of the receptor structure,..
  11. pmc Identification of functionally important residues of the rat P2X4 receptor by alanine scanning mutagenesis of the dorsal fin and left flipper domains
    Vendula Tvrdonova
    Department of Cellular and Molecular Neuroendocrinology, Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic Department of Physiology of Animals, Faculty of Science, Charles University, Prague, Czech Republic
    PLoS ONE 9:e112902. 2014
    Crystallization of the zebrafish P2X4 receptor in both open and closed states revealed conformational differences in the ectodomain structures, including the dorsal fin and left flipper domains...
  12. doi Involvement of the left-flipper-to-dorsal-fin interface of the zebrafish P2X4 receptor in ATP binding and structural rearrangement
    Yanling Jie
    Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Neurosci Lett 582:1-5. 2014
    ..In this way, we sought to determine the function of side chains of the zebrafish P2X4 receptor ectodomain left-flipper-to-dorsal-fin interface residues in ligand binding...
  13. pmc Imaging P2X4 receptor subcellular distribution, trafficking, and regulation using P2X4-pHluorin
    Ji Xu
    Department of Physiology and Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095
    J Gen Physiol 144:81-104. 2014
    b>P2X4 receptors are adenosine triphosphate (ATP)-gated cation channels present on the plasma membrane (PM) and also within intracellular compartments such as vesicles, vacuoles, lamellar bodies (LBs), and lysosomes...
  14. pmc Inherent dynamics of head domain correlates with ATP-recognition of P2X4 receptors: insights gained from molecular simulations
    Li Dong Huang
    Department of Pharmacology and Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    PLoS ONE 9:e97528. 2014
    ..Recent crystal structures of the zebrafish P2X4 (zfP2X4) receptor reveal a large ATP-binding pocket (ABP) located at the subunit interface of zfP2X4 receptors, ..
  15. pmc Inter- and intrasubunit interactions between transmembrane helices in the open state of P2X receptor channels
    Gabriel Heymann
    Molecular Physiology and Biophysics Section, Porter Neuroscience Research Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 110:E4045-54. 2013
    ..Apo and ATP-bound X-ray structures of the detergent-solubilized zebrafish P2X4 receptor provide a blueprint for receptor mechanisms but unexpectedly showed large crevices between subunits within ..
  16. pmc High potency zinc modulation of human P2X2 receptors and low potency zinc modulation of rat P2X2 receptors share a common molecular mechanism
    Sukanya Punthambaker
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 287:22099-111. 2012
    ..candidate regions controlling zinc inhibition in hP2X2 a homology model based on the crystal structure of zebrafish P2X4.1 was made. In this model, His-204 and His-209 of one subunit were near His-330 of the adjacent subunit...
  17. doi Salt bridge switching from Arg290/Glu167 to Arg290/ATP promotes the closed-to-open transition of the P2X2 receptor
    Ralf Hausmann
    Department of Molecular Pharmacology, RWTH Aachen University, Wendlingweg 2, D 52074 Aachen, Germany
    Mol Pharmacol 83:73-84. 2013
    ..In this study, we used homology modeling of the rat P2X2 receptor with the zebrafish P2X4 X-ray template to determine that the side chains of the Glu167 and Arg290 residues are in close spatial vicinity ..
  18. pmc Gating mechanism of a P2X4 receptor developed from normal mode analysis and molecular dynamics simulations
    Juan Du
    Department of Physics and Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA
    Proc Natl Acad Sci U S A 109:4140-5. 2012
    ..Our computational studies produced the first complete model, supported by experimental data, for how ATP binding triggers activation of a P2X receptor...
  19. doi Role of the ectodomain serine 275 in shaping the binding pocket of the ATP-gated P2X3 receptor
    Nataliia Petrenko
    Department of Neurobiology, AI Virtanen Institute, University of Eastern Finland, Kuopio, Finland
    Biochemistry 50:8427-36. 2011
    ..the rough structure of the ATP binding site has been proposed on the basis of the X-ray structure of the zebrafish P2X4 receptor and mutagenesis studies, the fine subunit-specific structural properties predisposing the receptor to ..
  20. pmc Expression, purification, electron microscopy, N-glycosylation mutagenesis and molecular modeling of human P2X4 and Dictyostelium discoideum P2XA
    Maria Valente
    School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, UK
    Biochim Biophys Acta 1808:2859-66. 2011
    The recent publication of the apo-, closed-state 3D crystal structure of zebrafish (zf) P2X4.1 has not only revolutionized the P2X research field, but also highlighted the need for further crystal structures, of receptors in different ..
  21. pmc Ion access pathway to the transmembrane pore in P2X receptor channels
    Toshimitsu Kawate
    Porter Neuroscience Research Center, Molecular Physiology and Biophysics Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Gen Physiol 137:579-90. 2011
    ..The x-ray structure of the P2X4 receptor from zebrafish (zfP2X4) receptor reveals that the extracellular vestibule above the gate opens to the ..
  22. pmc Polar residues in the second transmembrane domain of the rat P2X2 receptor that affect spontaneous gating, unitary conductance, and rectification
    Lishuang Cao
    Faculty of Medical and Human Sciences, University of Manchester, Manchester M13 9PL, UK
    J Neurosci 29:14257-64. 2009
    ..1 A crystal structure of the zebrafish P2X4.1 receptor in the closed state...
  23. ncbi Comparative expression of p2x receptors and ecto-nucleoside triphosphate diphosphohydrolase 3 in hypocretin and sensory neurons in zebrafish
    Lior Appelbaum
    Department of Psychiatry and Behavioral Sciences, Stanford University, Palo Alto, CA 94305, USA
    Brain Res 1174:66-75. 2007
    ..The conservation of expression of these genes in HCRT cells and sensory neurons across vertebrates suggests an important role for ATP mediated transmission in the regulation of sleep and the processing of sensory inputs...
  24. ncbi Molecular characterization of the zebrafish P2X receptor subunit gene family
    S Kucenas
    Department of Pharmacological and Physiological Science, St Louis University School of Medicine, 1402 South Grand Boulevard, St Louis, MO 63104, USA
    Neuroscience 121:935-45. 2003
    ..These results suggest that P2X receptors might play a role in the early development and/or function of the sensory nervous system in vertebrates...