mpx

Summary

Gene Symbol: mpx
Description: myeloid-specific peroxidase
Alias: fj80f04, mpo, wu:fj80f04, eosinophil peroxidase, drf, myeloperoxidase, unm t30963, unm_sm681, unm_t30963
Species: zebrafish

Top Publications

  1. pmc Haem homeostasis is regulated by the conserved and concerted functions of HRG-1 proteins
    Abbhirami Rajagopal
    Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA
    Nature 453:1127-31. 2008
  2. ncbi Myelopoiesis in the zebrafish, Danio rerio
    C M Bennett
    Department of Pediatric Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Blood 98:643-51. 2001
  3. ncbi Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish
    G J Lieschke
    Ludwig Institute for Cancer Research, The Royal Melbourne Hospital, Parkville, Victoria, Australia
    Blood 98:3087-96. 2001
  4. ncbi The pu.1 promoter drives myeloid gene expression in zebrafish
    Karl Hsu
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Blood 104:1291-7. 2004
  5. ncbi Zebrafish scl functions independently in hematopoietic and endothelial development
    Kimberly A Dooley
    Division of Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute and Harvard Medical School, Howard Hughes Medical Institute, Boston, MA 02115, USA
    Dev Biol 277:522-36. 2005
  6. ncbi Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish
    Jennifer Rhodes
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Dev Cell 8:97-108. 2005
  7. ncbi Loss of gata1 but not gata2 converts erythropoiesis to myelopoiesis in zebrafish embryos
    Jenna L Galloway
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital Boston, Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 8:109-16. 2005
  8. ncbi Loss of Hspa9b in zebrafish recapitulates the ineffective hematopoiesis of the myelodysplastic syndrome
    Sarah E Craven
    DNA Way, South San Francisco, CA 94080, USA
    Blood 105:3528-34. 2005
  9. ncbi Zebrafish as a model for myelopoiesis during embryogenesis
    Jason N Berman
    Department of Pediatric Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, Mass 02115, USA
    Exp Hematol 33:997-1006. 2005
  10. pmc Hematopoietic stem cell fate is established by the Notch-Runx pathway
    Caroline Erter Burns
    Stem Cell Program and Division of Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 19:2331-42. 2005

Scientific Experts

Detail Information

Publications122 found, 100 shown here

  1. pmc Haem homeostasis is regulated by the conserved and concerted functions of HRG-1 proteins
    Abbhirami Rajagopal
    Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA
    Nature 453:1127-31. 2008
    ..Thus, uncovering the mechanisms of haem transport in C. elegans may provide insights into human disorders of haem metabolism and reveal new drug targets for developing anthelminthics to combat worm infestations...
  2. ncbi Myelopoiesis in the zebrafish, Danio rerio
    C M Bennett
    Department of Pediatric Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Blood 98:643-51. 2001
    ..Zebrafish neutrophils resembled human neutrophils, possessing segmented nuclei and myeloperoxidase-positive cytoplasmic granules...
  3. ncbi Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish
    G J Lieschke
    Ludwig Institute for Cancer Research, The Royal Melbourne Hospital, Parkville, Victoria, Australia
    Blood 98:3087-96. 2001
    ..Embryonic zebrafish have circulating immature heterophils by 48 hours after fertilization (hpf). A zebrafish myeloperoxidase homologue (myeloid-specific peroxidase; mpx) was isolated...
  4. ncbi The pu.1 promoter drives myeloid gene expression in zebrafish
    Karl Hsu
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Blood 104:1291-7. 2004
    ..These transgenic lines will be useful to dissect the cellular and molecular control of myeloid cell differentiation, and this promoter fragment may prove useful in the development of zebrafish models of acute myeloid leukemia...
  5. ncbi Zebrafish scl functions independently in hematopoietic and endothelial development
    Kimberly A Dooley
    Division of Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute and Harvard Medical School, Howard Hughes Medical Institute, Boston, MA 02115, USA
    Dev Biol 277:522-36. 2005
    ..Surprisingly, in cloche, lmo2 was not induced in response to scl over-expression. Taken together, these findings support distinct roles for scl in hematopoietic and endothelial development, downstream of hemangioblast development...
  6. ncbi Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish
    Jennifer Rhodes
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Dev Cell 8:97-108. 2005
    ..1 in the bloodless mutant cloche resulted in myelopoietic rescue, providing intriguing evidence that this gene can function in the absence of some stem cell genes, such as scl, in governing myelopoiesis...
  7. ncbi Loss of gata1 but not gata2 converts erythropoiesis to myelopoiesis in zebrafish embryos
    Jenna L Galloway
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital Boston, Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 8:109-16. 2005
    ..Our studies establish a transcriptional hierarchy of Gata factor dependence during hematopoiesis and demonstrate that gata1 plays an integral role in directing myelo-erythroid lineage fate decisions during embryogenesis...
  8. ncbi Loss of Hspa9b in zebrafish recapitulates the ineffective hematopoiesis of the myelodysplastic syndrome
    Sarah E Craven
    DNA Way, South San Francisco, CA 94080, USA
    Blood 105:3528-34. 2005
    ..Thus, we identify an essential role for Hspa9b in hematopoiesis and implicate both loss of HSPA9B specifically and mitochondrial dysfunction generally in the pathogenesis of the MDS...
  9. ncbi Zebrafish as a model for myelopoiesis during embryogenesis
    Jason N Berman
    Department of Pediatric Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, Mass 02115, USA
    Exp Hematol 33:997-1006. 2005
    ..The conservation of genes such as scl, pu.1, c/ebpalpha, mpo, l-plastin, and lysozyme C in myelopoiesis and the corresponding expression patterns in zebrafish suggests that ..
  10. pmc Hematopoietic stem cell fate is established by the Notch-Runx pathway
    Caroline Erter Burns
    Stem Cell Program and Division of Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 19:2331-42. 2005
    ..These data define the Notch-Runx pathway as critical for the developmental specification of HSC fate and the subsequent homeostasis of HSC number, thus providing a mechanism for amplifying stem cells in vivo...
  11. ncbi Specification of the primitive myeloid precursor pool requires signaling through Alk8 in zebrafish
    Benjamin M Hogan
    Ludwig Institute for Cancer Research, Post Office Box 2008, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia
    Curr Biol 16:506-11. 2006
    ..1 expression, but the role of alk8 was independent of the scl-dependent cell-fate pathway. Furthermore, the role of Alk8 in myelopoiesis was genetically separable from its earlier role in dorsal-ventral embryonic patterning...
  12. ncbi A transgenic zebrafish model of neutrophilic inflammation
    Stephen A Renshaw
    MRC Centre for Developmental and Biomedical Genetics, Acadamic Unit of Respiratory Medicine, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    Blood 108:3976-8. 2006
    ..response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter...
  13. pmc The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish
    Chris Hall
    Department of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Auckland, New Zealand
    BMC Dev Biol 7:42. 2007
    ..A number of blood-specific lines have provided a means to exploit the exquisite optical clarity that this vertebrate system offers and provide a level of insight into dynamic inflammatory processes previously unavailable...
  14. pmc Heat shock-inducible Cre/Lox approaches to induce diverse types of tumors and hyperplasia in transgenic zebrafish
    Xiuning Le
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:9410-5. 2007
    ..Moreover, these tumors and hyperplasia closely resemble human diseases at both the morphologic and molecular levels...
  15. ncbi Identification and real-time imaging of a myc-expressing neutrophil population involved in inflammation and mycobacterial granuloma formation in zebrafish
    Annemarie H Meijer
    Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands
    Dev Comp Immunol 32:36-49. 2008
    ..showing that lcp1 can be considered as a general leukocyte marker, csf1r as a macrophage-specific marker, and mpx and lyz as neutrophil-specific markers...
  16. pmc Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis
    Trista E North
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 447:1007-11. 2007
    ..The conserved role for PGE2 in the regulation of vertebrate HSC homeostasis indicates that modulation of the prostaglandin pathway may facilitate expansion of HSC number for therapeutic purposes...
  17. ncbi Inactivation of serine protease Matriptase1a by its inhibitor Hai1 is required for epithelial integrity of the zebrafish epidermis
    Thomas J Carney
    Max Planck Institute of Immunobiology, Stuebeweg 51, D 79108 Freiburg, Germany
    Development 134:3461-71. 2007
    ..Our work provides direct genetic evidence for antagonistic in vivo roles of Hai1 and Matriptase1a to regulate skin homeostasis and remodeling...
  18. ncbi Live imaging of chronic inflammation caused by mutation of zebrafish Hai1
    Jonathan R Mathias
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Cell Sci 120:3372-83. 2007
    ..These findings illustrate the utility of zebrafish as a new model system to study chronic inflammation and to visualize immune responses with high resolution in vivo...
  19. pmc Dichotomous role of the macrophage in early Mycobacterium marinum infection of the zebrafish
    Hilary Clay
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98195, USA
    Cell Host Microbe 2:29-39. 2007
    ..We propose that residence within macrophages represents an evolutionary trade-off for pathogenic mycobacteria that slows their early growth but provides a mechanism for tissue dissemination...
  20. ncbi AML1-ETO reprograms hematopoietic cell fate by downregulating scl expression
    Jing Ruey J Yeh
    Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Development 135:401-10. 2008
    ..Thus, this zebrafish model facilitates in vivo dissection of AML1-ETO-mediated signaling, and will enable large-scale chemical screens to identify suppressors of the in vivo effects of AML1-ETO...
  21. pmc Interplay among Etsrp/ER71, Scl, and Alk8 signaling controls endothelial and myeloid cell formation
    Saulius Sumanas
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, USA
    Blood 111:4500-10. 2008
    ..We further demonstrate that the choice of endothelial versus myeloid fate depends on a combinatorial effect of etsrp, scl, and alk8 genes...
  22. doi eena Promotes myeloid proliferation through stimulating ERK1/2 phosphorylation in zebrafish
    Huang Ying Le
    Laboratory of Development and Diseases and State Key Laboratory of Medical Genomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    J Biol Chem 283:17652-61. 2008
    ..The results suggest that eena plays an important role in the development of the myeloid cell through activation of the ERK pathway and may provide a valuable reference for future studies of the role of EEN in leukemogenesis...
  23. pmc CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis
    Julien Y Bertrand
    Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0380, USA
    Development 135:1853-62. 2008
    ..Together, these studies suggest that expression of CD41 and cmyb marks nascent HSCs in the zebrafish AGM, and provide the means to further dissect HSC generation and function in the early vertebrate embryo...
  24. ncbi In vivo interstitial migration of primitive macrophages mediated by JNK-matrix metalloproteinase 13 signaling in response to acute injury
    Yong Zhang
    Laboratory of Development and Diseases and State Key Laboratory for Medical Genomics and Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    J Immunol 181:2155-64. 2008
    ..The results indicate that the JNK-Mmp13 signaling pathway plays an essential role in regulating the innate immune cell migration in response to severe injury in vivo...
  25. doi Deficiency of ribosomal protein S19 during early embryogenesis leads to reduction of erythrocytes in a zebrafish model of Diamond-Blackfan anemia
    Tamayo Uechi
    Frontier Science Research Center, University of Miyazaki, Miyazaki 889 1692, Japan
    Hum Mol Genet 17:3204-11. 2008
    ..The RPS19-deficient zebrafish will provide a valuable tool for investigating the molecular mechanisms of DBA development in humans...
  26. pmc A non-canonical function of zebrafish telomerase reverse transcriptase is required for developmental hematopoiesis
    Shintaro Imamura
    Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, USA
    PLoS ONE 3:e3364. 2008
    ..The data also provide insights into a non-canonical pathway by which TERT functions to modulate specification of hematopoietic stem/progenitor cells during vertebrate development. (276 words)...
  27. pmc Muscle degeneration and leukocyte infiltration caused by mutation of zebrafish Fad24
    Kevin B Walters
    Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Dev Dyn 238:86-99. 2009
    ....
  28. doi Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration
    Clifford Liongue
    School of Medicine, Deakin University, Waurn Ponds, Vic, Australia
    Blood 113:2535-46. 2009
    ....
  29. pmc Hematopoietic stem cell development is dependent on blood flow
    Trista E North
    Stem Cell Program and Hematology Oncology, Children s Hospital, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 137:736-48. 2009
    ..This work links blood flow to AGM hematopoiesis and identifies NO as a conserved downstream regulator of HSC development...
  30. pmc The ENTH domain protein Clint1 is required for epidermal homeostasis in zebrafish
    M Ernest Dodd
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA
    Development 136:2591-600. 2009
    ....
  31. pmc Characterization of zebrafish larval inflammatory macrophages
    Jonathan R Mathias
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    Dev Comp Immunol 33:1212-7. 2009
    ..These macrophages can be distinguished from neutrophils by immunolabeling of L-Plastin without MPO co-expression and by an elongated morphology...
  32. pmc Differential regulation of protrusion and polarity by PI3K during neutrophil motility in live zebrafish
    Sa Kan Yoo
    Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, WI 53706, USA
    Dev Cell 18:226-36. 2010
    ..This work provides new insight into how cell signaling at the front and back of the cell is coordinated during polarized cell migration in intact tissues within a multicellular organism...
  33. doi An evolutionarily conserved PTEN-C/EBPalpha-CTNNA1 axis controls myeloid development and transformation
    Chun Tang Fu
    Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People s Republic of China
    Blood 115:4715-24. 2010
    ..This study uncovers a novel molecular pathway, PTEN-C/EBPalpha-CTNNA1, which is evolutionarily conserved and might be therapeutically targeted to eradicate LICs with low CTNNA1 expression...
  34. pmc Essential role of c-myb in definitive hematopoiesis is evolutionarily conserved
    Cristian Soza-Ried
    Department of Developmental Immunology, Max Planck Institute of Immunobiology, D 79108 Freiburg, Germany
    Proc Natl Acad Sci U S A 107:17304-8. 2010
    ..Our results, therefore, suggest that the key role of c-myb in definitive hematopoiesis is similar to that in mammals and must have become established early in vertebrate evolution...
  35. doi Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis
    Li Li
    State Key Laboratory of Molecular Neuroscience, Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People s Republic of China
    Blood 117:1359-69. 2011
    ..1 but is insufficient to promote macrophage development in the absence of Pu.1. Our findings demonstrate that Irf8 is a critical determinant for neutrophil versus macrophage fate choice during zebrafish primitive myelopoiesis...
  36. pmc mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish
    Felix Ellett
    Cancer and Haematology Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
    Blood 117:e49-56. 2011
    ..These zebrafish transgenes provide a new resource that will contribute to the fields of inflammation, infection, and leukocyte biology...
  37. doi Topographical distribution of antimicrobial genes in the zebrafish intestine
    Stefan H Oehlers
    Department of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Private Bag 92019, Auckland Mail Centre, Auckland 1142, New Zealand
    Dev Comp Immunol 35:385-91. 2011
    ..Expression of the zebrafish β-defensin family was examined in adult zebrafish tissues. Strong expression of defensin beta-like 1 was detected in the swim bladder of zebrafish from the larval stage of development through to adults...
  38. pmc Spatiotemporal photolabeling of neutrophil trafficking during inflammation in live zebrafish
    Sa Kan Yoo
    University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Leukoc Biol 89:661-7. 2011
    ..This work provides new insight into neutrophil behaviors during inflammation and resolution within a multicellular organism...
  39. pmc Clonal analysis of hematopoietic progenitor cells in the zebrafish
    David L Stachura
    Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093 0380, USA
    Blood 118:1274-82. 2011
    ..Analysis of the gene-expression profiles after isolation of colonies marked by gata1:DsRed or mpx:eGFP transgenes confirmed our morphological erythroid and myeloid lineage designations, respectively...
  40. pmc Microbial colonization induces dynamic temporal and spatial patterns of NF-κB activation in the zebrafish digestive tract
    Michelle Kanther
    Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, North Carolina, USA
    Gastroenterology 141:197-207. 2011
    ..We used in vivo imaging and molecular approaches to analyze NF-κB activation in response to the commensal microbiota in transparent gnotobiotic zebrafish...
  41. doi Dominant-negative C/ebpα and polycomb group protein Bmi1 extend short-lived hematopoietic stem/progenitor cell life span and induce lethal dyserythropoiesis
    Ting Zhou
    Key Laboratory of Stem Cell Biology and State Key Laboratory of Medical Genomics and Laboratory of Development and Diseases, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, and Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People s Republic of China
    Blood 118:3842-52. 2011
    ..The results indicate that Bmi1 acts immediately downstream of C/ebpαDN to regulate the survival and self-renewal of HSPCs and contribute to the erythropoietic dysplasia...
  42. pmc Dual roles for Rac2 in neutrophil motility and active retention in zebrafish hematopoietic tissue
    Qing Deng
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA
    Dev Cell 21:735-45. 2011
    ....
  43. pmc Lyn is a redox sensor that mediates leukocyte wound attraction in vivo
    Sa Kan Yoo
    Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Nature 480:109-12. 2011
    ..This is the first identification, to our knowledge, of a physiological redox sensor that mediates leukocyte wound attraction in multicellular organisms...
  44. pmc Hematopoietic defects in rps29 mutant zebrafish depend upon p53 activation
    Alison M Taylor
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Mass, USA
    Exp Hematol 40:228-237.e5. 2012
    ..Together, our studies demonstrate a role of p53 in mediating the cellular defects associated with rps29 and establish a role for rps29 and p53 in hematopoietic stem cells and red blood cell development...
  45. pmc Zebrafish models for dyskeratosis congenita reveal critical roles of p53 activation contributing to hematopoietic defects through RNA processing
    Ying Zhang
    Laboratory of Chemical Genomics, Shenzhen Graduate School, Peking University, Shenzhen, China
    PLoS ONE 7:e30188. 2012
    ....
  46. doi Infection-responsive expansion of the hematopoietic stem and progenitor cell compartment in zebrafish is dependent upon inducible nitric oxide
    Christopher J Hall
    Department of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand
    Cell Stem Cell 10:198-209. 2012
    ..These results provide new insights into the reactive capacity of HSPCs and how the blood system is "fine-tuned" in response to inflammatory stress...
  47. doi Developmental nephrotoxicity of aristolochic acid in a zebrafish model
    Yu Ju Ding
    Department of Chemistry, Tamkang University, Tamsui, New Taipei City, Taiwan
    Toxicol Appl Pharmacol 261:59-65. 2012
    ..Real-time PCR showed that AA can up-regulate the expression of proinflammatory genes like TNFα, cox2 and mpo. These results support the following conclusions: (1) AA-induced renal failure is mediated by inflammation, which ..
  48. pmc In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma
    Myron S Ignatius
    Department of Pathology and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Cancer Cell 21:680-93. 2012
    ..Our data suggest that non-tumor-propagating cells likely have important supportive roles in cancer progression and facilitate metastasis...
  49. pmc Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development
    Elayne Provost
    Department of Surgery, Johns Hopkins University, Baltimore, MD 21205, USA
    Development 139:3232-41. 2012
    ..The pancreatic phenotypes observed in rpl3- and pes-deficient embryos were also independent of p53. Together, these data suggest novel p53-independent roles for ribosomal biogenesis genes in zebrafish pancreas development...
  50. doi Neutrophil-delivered myeloperoxidase dampens the hydrogen peroxide burst after tissue wounding in zebrafish
    Luke Pase
    Cancer and Haematology Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
    Curr Biol 22:1818-24. 2012
    ..Neutrophils carry abundant amounts of myeloperoxidase, an enzyme catalyzing an H(2)O(2)-consuming reaction [7, 8]...
  51. pmc The SH2-domain-containing inositol 5-phosphatase (SHIP) limits the motility of neutrophils and their recruitment to wounds in zebrafish
    Pui Ying Lam
    Department of Medical Microbiology and Immunology University of Wisconsin Madison, Madison, WI 53706, USA
    J Cell Sci 125:4973-8. 2012
    ..Moreover, overexpression of the SHIP phosphatase domain in neutrophils impaired neutrophil 3D migration. Taken together, our findings suggest that SHIP phosphatases control neutrophil inflammation by limiting neutrophil motility in vivo...
  52. pmc Maternal pak4 expression is required for primitive myelopoiesis in zebrafish
    Sheran H W Law
    Section on Vertebrate Development, Program on Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Development, National Institutes of Health, Bethesda, MD, USA
    Mech Dev 130:181-94. 2013
    ....
  53. doi Phospholipase C gamma-1 is required for granulocyte maturation in zebrafish
    Chang Bin Jing
    Key Laboratory of Stem Cell Biology, Laboratory of Development and Diseases, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, People s Republic of China
    Dev Biol 374:24-31. 2013
    ..mutant that has relatively normal hematopoiesis, except decreased expression of the granulocyte-specific gene mpx. Positional cloning revealed that phospholipase C gamma-1 (plcg1) was mutated...
  54. pmc Localized bacterial infection induces systemic activation of neutrophils through Cxcr2 signaling in zebrafish
    Qing Deng
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Leukoc Biol 93:761-9. 2013
    ....
  55. pmc An anti-inflammatory NOD-like receptor is required for microglia development
    Celia E Shiau
    Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA
    Cell Rep 5:1342-52. 2013
    ..Together, these results demonstrate that NLRC3-like prevents inappropriate macrophage activation, thereby allowing normal microglial development. ..
  56. pmc Heat shock modulates neutrophil motility in zebrafish
    Pui Ying Lam
    Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, Wisconsin, United States of America Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 8:e84436. 2013
    ..Our results indicate that routine heat shock can alter neutrophil function in zebrafish. The findings suggest that caution should be taken when employing a heat shock-dependent inducible system to study the innate immune response. ..
  57. ncbi Resolution of inflammation by retrograde chemotaxis of neutrophils in transgenic zebrafish
    Jonathan R Mathias
    University of Wisconsin Madison, 2715 Medical Sciences Center, 1300 University Ave, Madison, WI 53706, USA
    J Leukoc Biol 80:1281-8. 2006
    ..we have developed a novel transgenic zebrafish in which the neutrophils express GFP under control of the myeloperoxidase promoter (zMPO:GFP)...
  58. pmc Tnfa signaling through tnfr2 protects skin against oxidative stress-induced inflammation
    Sergio Candel
    Departamento de Biología Celular e Histología, Facultad de Biologia, Universidad de Murcia, Murcia, Spain Instituto Murciano de Investigación Biosanitaria IMIB, Murcia, Spain
    PLoS Biol 12:e1001855. 2014
    ..These results reveal a crucial role for TNFα/TNFR2 axis in the protection of the skin against DUOX1-mediated oxidative stress and could establish new therapeutic targets for skin inflammatory disorders...
  59. pmc CBFβ and RUNX1 are required at 2 different steps during the development of hematopoietic stem cells in zebrafish
    Erica Bresciani
    Oncogenesis and Development Section
    Blood 124:70-8. 2014
    ..Our results also indicate that RUNX1 can drive the emergence of nascent HSCs in the AGM without its heterodimeric partner CBFβ. ..
  60. doi Notable mucosal immune responses induced in the intestine of zebrafish (Danio rerio) bath-vaccinated with a live attenuated Vibrio anguillarum vaccine
    Xiaohong Liu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Fish Shellfish Immunol 40:99-108. 2014
    ..In conclusion, the live attenuated V. anguillarum vaccine induced notable mucosal immune responses in the intestine which could be used as a mucosal vaccine vector in the future...
  61. pmc Differential requirement for irf8 in formation of embryonic and adult macrophages in zebrafish
    Celia E Shiau
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, California, United States of America
    PLoS ONE 10:e0117513. 2015
    ..Our study defines distinct requirement for irf8 in myelopoiesis before and after transition to the adult hematopoietic system. ..
  62. pmc Reticular dysgenesis-associated AK2 protects hematopoietic stem and progenitor cell development from oxidative stress
    Alberto Rissone
    Disorders of Immunity Section, Genetics and Molecular Biology Branch Zebrafish Core and Oncogenesis and Development Section, Translational and Functional Genomics Branch Genomics Core, Cancer Genetics and Comparative Genomics Branch Division of Intramural Research Flow Cytometry Core and Cytogenetics and Microscopy Core, Genetic Disease Research Branch National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892
    J Exp Med 212:1185-202. 2015
    ..Our results link hematopoietic cell fate in AK2 deficiency to cellular energy depletion and increased oxidative stress. This points to the potential use of antioxidants as a supportive therapeutic modality for patients with RD. ..
  63. pmc An evolutionarily conserved program of B-cell development and activation in zebrafish
    Dawne M Page
    Department of Biology, Point Loma Nazarene University, San Diego, CA 92106, USA
    Blood 122:e1-11. 2013
    ..The IgM1:eGFP reporter fish are the first nonmammalian B-cell reporter animals to be described. They will be important for further investigation of immune cell evolution and development and host-pathogen interactions in zebrafish. ..
  64. pmc Notch signaling distinguishes 2 waves of definitive hematopoiesis in the zebrafish embryo
    Julien Y Bertrand
    Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0380, USA
    Blood 115:2777-83. 2010
    ..These studies establish that HSCs are the only hematopoietic precursor that requires Notch signaling and help to clarify the signaling events underlying the specification of the 2 distinct waves of definitive hematopoiesis...
  65. pmc Broad-host-range plasmids for red fluorescent protein labeling of gram-negative bacteria for use in the zebrafish model system
    John T Singer
    Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USA
    Appl Environ Microbiol 76:3467-74. 2010
    ..Results from zebrafish infectivity studies indicate that RFP-labeled pathogens will be useful for the study of real-time interactions between host cells of the innate immune system and the infecting pathogen...
  66. pmc cpsf1 is required for definitive HSC survival in zebrafish
    Niccolò Bolli
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, USA
    Blood 117:3996-4007. 2011
    ..These studies show that cpsf1 is essential for HSC survival and differentiation in caudal hematopoietic tissue...
  67. pmc The NOTCH pathway contributes to cell fate decision in myelopoiesis
    Laurence Bugeon
    Imperial College London, London, SW7 2AZ, UK
    Haematologica 96:1753-60. 2011
    ....
  68. pmc Retinoic acid signaling plays a restrictive role in zebrafish primitive myelopoiesis
    Dong Liang
    Model Animal Research Center, MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China
    PLoS ONE 7:e30865. 2012
    ..Taken together, our results demonstrate that RA signaling restricts zebrafish primitive myelopoiesis through acting downstream of gata4/5/6, upstream of, or parallel to, cloche, and upstream of scl...
  69. doi Zebrafish (Danio rerio) larvae as a system to test the efficacy of polysaccharides as immunostimulants
    U Oyarbide
    AZTI Tecnalia, Food Research Division, Parque Tecnologico de Bizkaia, Derio Bizkaia, Spain
    Zebrafish 9:74-84. 2012
    ..The most evident effects of β-glucan were the overexpression of the genes TNFα, MPO, TRF, and LYZ...
  70. pmc Runx1 regulates embryonic myeloid fate choice in zebrafish through a negative feedback loop inhibiting Pu.1 expression
    Hao Jin
    State Key Laboratory of Molecular Neuroscience, Division of Life Science, Hong Kong University of Science and Technology, Hong Kong, PR China
    Blood 119:5239-49. 2012
    ..1 level required for balanced fate commitment to neutrophils versus macrophages. Our findings define a Pu.1-Runx1 regulatory loop that governs the equilibrium between distinct myeloid fates by assuring an appropriate Pu.1 dosage...
  71. doi The role of Nfil3 in zebrafish hematopoiesis
    Fränze Progatzky
    Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, London, UK
    Dev Comp Immunol 38:187-92. 2012
    ..Using mpx and fms-fluorescent transgenic fish we found that all myeloid cell lineages, and in particular macrophages, had ..
  72. pmc Persistent oxytetracycline exposure induces an inflammatory process that improves regenerative capacity in zebrafish larvae
    Francisco Barros-Becker
    Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile
    PLoS ONE 7:e36827. 2012
    ..In this work, we use the zebrafish to analyze quantitatively the effects of persistent exposure to oxytetracycline, the most common antibiotic used in fish farming...
  73. pmc Teleost growth factor independence (gfi) genes differentially regulate successive waves of hematopoiesis
    Jeffrey D Cooney
    Department of Medicine, Division of Hematology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Dev Biol 373:431-41. 2013
    ..Our studies establish a comparative paradigm for the regulation of hematopoietic lineages by gfi transcription factors...
  74. doi Rac2 expression and its role in neutrophil functions of zebrafish (Danio rerio)
    Rachel M Tell
    Immunobiology Graduate Program, 1068 Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Fish Shellfish Immunol 33:1086-94. 2012
    ..The expression pattern and role of Rac2 in zebrafish neutrophil function allows for comparative studies of innate immune responses in this animal model...
  75. pmc Hematopoiesis
    Madhumita Jagannathan-Bogdan
    HHMI Children s Hospital Boston, Boston, MA 02115, USA
    Development 140:2463-7. 2013
    ..Here, we provide a broad overview of vertebrate hematopoiesis. We also highlight the benefits of using zebrafish as a model...
  76. pmc Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota
    John F Rawls
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63108, USA
    Proc Natl Acad Sci U S A 101:4596-601. 2004
    ..Together, these studies establish gnotobiotic zebrafish as a useful model for dissecting the molecular foundations of host-microbial interactions in the vertebrate digestive tract...
  77. ncbi Immune-related, lectin-like receptors are differentially expressed in the myeloid and lymphoid lineages of zebrafish
    Patoula G Panagos
    Department of Biology, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA
    Immunogenetics 58:31-40. 2006
    ..Illr genes are differentially expressed in the myeloid and lymphoid lineages, suggesting that they may play important roles in the immune functions of multiple hematopoietic cell lineages...
  78. pmc Fgf21 is essential for haematopoiesis in zebrafish
    Hajime Yamauchi
    Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Yoshida Shimoadachi, Sakyo, Kyoto 606 8501, Japan
    EMBO Rep 7:649-54. 2006
    ..These results indicate that Fgf21 is a newly identified factor essential for the determination of myelo-erythroid progenitor cell fate in vivo...
  79. ncbi BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis
    Sunny Gupta
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Development 133:2177-87. 2006
    ....
  80. ncbi Antisense targeting of CXXC finger protein 1 inhibits genomic cytosine methylation and primitive hematopoiesis in zebrafish
    Suzanne R L Young
    Herman B Wells Center for Pediatric Research, Section of Pediatric Hematology Oncology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Biol Chem 281:37034-44. 2006
    ..These findings establish the importance of CFP1 during postgastrulation development...
  81. pmc Reciprocal gut microbiota transplants from zebrafish and mice to germ-free recipients reveal host habitat selection
    John F Rawls
    Center for Genome Sciences, Washington University School of Medicine, St Louis, MO 63108, USA
    Cell 127:423-33. 2006
    ....
  82. pmc In vivo imaging and genetic analysis link bacterial motility and symbiosis in the zebrafish gut
    John F Rawls
    Center for Genome Sciences, Washington University School of Medicine, St Louis, MO 63108, USA
    Proc Natl Acad Sci U S A 104:7622-7. 2007
    ....
  83. pmc Structural characteristics of zebrafish orthologs of adaptor molecules that associate with transmembrane immune receptors
    Jeffrey A Yoder
    Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    Gene 401:154-64. 2007
    ..Their genomic organizations have been characterized, cDNA transcripts have been recovered, phylogenetic relationships have been defined and their cell lineage-specific expression patterns have been established...
  84. ncbi Innate immune gene expression in individual zebrafish after Listonella anguillarum inoculation
    Irune Rojo
    AZTI Tecnalia, Food Research Unit, Txatxarramendi Ugartea z g, E 48395 Sukarrieta, Bizkaia, Spain
    Fish Shellfish Immunol 23:1285-93. 2007
    ..2, 4, 6 and 22h post infection (hpi) and the expression of genes related to the immune response (il1b, cebpb, tfa, mpx, tnfa, nitr9, tlr22, hsc70, cp, mrlp1, c3b and lyz) in each fish was monitored by means of real-time RT-PCR...
  85. pmc The zebrafish activating immune receptor Nitr9 signals via Dap12
    Sheng Wei
    Immunology Program, H Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Avenue, Tampa, FL 33612, USA
    Immunogenetics 59:813-21. 2007
    ....
  86. doi Multiplex fluorescent in situ hybridization in zebrafish embryos using tyramide signal amplification
    Hilary Clay
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington 98195, USA
    Zebrafish 2:105-11. 2005
    ..To this effect, we demonstrate that this approach can be combined with standard horseradish peroxidase (HRP)-mediated immunocytochemistry procedures in addition to FISH...
  87. pmc Loss of TLE1 and TLE4 from the del(9q) commonly deleted region in AML cooperates with AML1-ETO to affect myeloid cell proliferation and survival
    Farshid Dayyani
    Department of Pediatrics, Division of Pediatric Hematology Oncology, Massachusetts General Hospital, and Harvard Medical School, Boston, MA 02114, USA
    Blood 111:4338-47. 2008
    ..This study is the first to implicate the TLEs as potential tumor suppressor genes in myeloid leukemia...
  88. doi A role for the transcription intermediary factor 2 in zebrafish myelopoiesis
    Julia Zhuravleva
    INSERM, University of Burgundy, Dijon, France
    Exp Hematol 36:559-67. 2008
    ..Therefore, we decided to choose the zebrafish model to study TIF2. The aim of this study was to characterize the role of this HAT during embryonic development...
  89. doi A zebrafish model for the Shwachman-Diamond syndrome (SDS)
    Narayanan Venkatasubramani
    Department of Pediatrics and Children s Research Institute, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Pediatr Res 63:348-52. 2008
    ..We also noted granulopoiesis defect using myeloperoxidase as a marker...
  90. pmc Chk1 suppresses a caspase-2 apoptotic response to DNA damage that bypasses p53, Bcl-2, and caspase-3
    Samuel Sidi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 133:864-77. 2008
    ..The evolutionarily conserved "Chk1-suppressed" pathway defines a novel apoptotic process, whose responsiveness to Chk1 inhibitors and insensitivity to p53 and BCL2 alterations have important implications for cancer therapy...
  91. doi Zebrafish runx1 promoter-EGFP transgenics mark discrete sites of definitive blood progenitors
    Enid Yi Ni Lam
    Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland, Auckland, New Zealand
    Blood 113:1241-9. 2009
    ..The ability to visualize and isolate fluorescently labeled HSCs should contribute to further elucidating the complex regulation of HSC development...
  92. doi Gene expression and functional analysis of zebrafish larval fin fold regeneration
    Nozomi Yoshinari
    Department of Biological Information, Tokyo Institute of Technology, Midori ku, Yokohama 226 8501, Japan
    Dev Biol 325:71-81. 2009
    ..Thus, our results demonstrate that the regeneration model of juvenile zebrafish offers a powerful assay to dissect the regeneration processes...
  93. pmc Essential role of spi-1-like (spi-1l) in zebrafish myeloid cell differentiation
    Alex Bukrinsky
    Department of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095 1606, USA
    Blood 113:2038-46. 2009
    ..1 cells and its function is necessary and sufficient for macrophage and granulocyte differentiation. These results establish a critical role for spi-1l in zebrafish myeloid cell differentiation...
  94. doi Fluorescence-activated cell sorting (FACS) of whole mount in situ hybridization (WISH) labelled haematopoietic cell populations in the zebrafish
    Joseph T Dobson
    IWK Health Centre, Dalhousie University, Halifax, NS, Canada
    Br J Haematol 144:732-5. 2009
    ....
  95. doi The role of survivin2 in primitive hematopoiesis during zebrafish development
    A C H Ma
    Division of Haematology and Bone Marrow Transplantation, Department of Medicine, Queen Mary Hospital, University of Hong Kong, Hong Kong, China
    Leukemia 23:712-20. 2009
    ..1) and late (mpo, l-plastin) myelomonocytic lineages at 12 and 18 h.p.f...
  96. pmc Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI
    Hao Jin
    Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, People s Republic of China
    Development 136:647-54. 2009
    ..Our results highlight the importance of niche in shaping the differentiation output of developing HSPCs...
  97. pmc Discovering chemical modifiers of oncogene-regulated hematopoietic differentiation
    Jing Ruey J Yeh
    Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Nat Chem Biol 5:236-43. 2009
    ..This approach may open doors for developing therapeutics targeting oncogene function within leukemic stem cells...
  98. pmc Fate mapping embryonic blood in zebrafish: multi- and unipotential lineages are segregated at gastrulation
    Rachel M Warga
    Department of Biological Sciences, Western Michigan University, Kalamazoo, MI 49008, USA
    Dev Cell 16:744-55. 2009
    ..Our results demonstrate the existence of primitive hematopoietic progenitors that are segregated very early in development and that are specified to produce either a unipotent or a multipotent blood cell lineage...
  99. doi Identification of phagocytic cells, NK-like cytotoxic cell activity and the production of cellular exudates in the coelomic cavity of adult zebrafish
    Lauren D Moss
    Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA
    Dev Comp Immunol 33:1077-87. 2009
    ..E. coli injected into the CC were phagocytosed in a dose and time dependent fashion by granulocytes, monocytes and macrophages. NCC lysed mammalian target cells and NCAMP-1 expressing hybridoma cells in redirected lysis assays...
  100. pmc Emi1 maintains genomic integrity during zebrafish embryogenesis and cooperates with p53 in tumor suppression
    Jennifer Rhodes
    Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111, USA
    Mol Cell Biol 29:5911-22. 2009
    ..Our results cast Emi1 as a critical regulator of genomic fidelity during embryogenesis and suggest that the factor may act as a tumor suppressor...
  101. doi Translocator protein (18 kDa) is involved in primitive erythropoiesis in zebrafish
    Christine Rampon
    CNRS UMR 8542, Chaire des Processes Morphogénètiques, College de France, 11 place Marcelin Berthelot, 75231 Paris Cedex 05, France
    FASEB J 23:4181-92. 2009
    ..Although unexpected, this lack of blood cells is independent of the Tspo cholesterol binding site and reveals a new in vivo key role for Tspo during erythropoiesis...