kdrl

Summary

Gene Symbol: kdrl
Description: kinase insert domain receptor like
Alias: flk, flk-1, flk1, kdr, kdra, vegfr-2, vegfr2, vegfr2a, vegfr4, vegr2, wu:fk52c05, vascular endothelial growth factor receptor kdr-like, VEGFR-4, fetal liver kinase 1, flt1b, kinase insert domain receptor-A, protein-tyrosine kinase receptor flk-1, vascular endothelial growth factor receptor 2, vascular endothelial growth factor receptor 4, vascular endothelial growth factor receptor type 2
Species: zebrafish

Top Publications

  1. ncbi Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos
    Anskar Y H Leung
    Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Dev Biol 277:235-54. 2005
  2. ncbi Thalidomide-induced antiangiogenic action is mediated by ceramide through depletion of VEGF receptors, and is antagonized by sphingosine-1-phosphate
    Takeshi Yabu
    Department of Hematology Oncology, Graduate School of Medicine, Kyoto University, Japan
    Blood 106:125-34. 2005
  3. pmc Notch signaling functions as a cell-fate switch between the endothelial and hematopoietic lineages
    Christina Y Lee
    McAllister Heart Institute, Department of Cell and Molecular Physiology, and Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Biol 19:1616-22. 2009
  4. pmc Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis
    Trista E North
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 447:1007-11. 2007
  5. pmc Haematopoietic stem cells derive directly from aortic endothelium during development
    Julien Y Bertrand
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093 0380, USA
    Nature 464:108-11. 2010
  6. pmc Hematopoietic stem cell fate is established by the Notch-Runx pathway
    Caroline Erter Burns
    Stem Cell Program and Division of Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 19:2331-42. 2005
  7. ncbi Lmo2 and Scl/Tal1 convert non-axial mesoderm into haemangioblasts which differentiate into endothelial cells in the absence of Gata1
    Martin Gering
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Development 130:6187-99. 2003
  8. ncbi Zebrafish scl functions independently in hematopoietic and endothelial development
    Kimberly A Dooley
    Division of Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute and Harvard Medical School, Howard Hughes Medical Institute, Boston, MA 02115, USA
    Dev Biol 277:522-36. 2005
  9. pmc Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation
    Shane P Herbert
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, Cardiovascular Research Institute, University of California, San Francisco, CA 94158, USA
    Science 326:294-8. 2009
  10. doi Two functional microRNA-126s repress a novel target gene p21-activated kinase 1 to regulate vascular integrity in zebrafish
    Jun Zou
    Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, China
    Circ Res 108:201-9. 2011

Research Grants

Scientific Experts

Detail Information

Publications130 found, 100 shown here

  1. ncbi Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos
    Anskar Y H Leung
    Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Dev Biol 277:235-54. 2005
    ..BMP4, but not BMP2b or 7, was involved in this process. The results provide ground for further research into the mechanisms of embryonic hematopoietic cell expansion...
  2. ncbi Thalidomide-induced antiangiogenic action is mediated by ceramide through depletion of VEGF receptors, and is antagonized by sphingosine-1-phosphate
    Takeshi Yabu
    Department of Hematology Oncology, Graduate School of Medicine, Kyoto University, Japan
    Blood 106:125-34. 2005
    ..loss and confirmed by the depletion of vascular endothelial growth factor (VEGF) receptors such as neuropilin-1 and Flk-1...
  3. pmc Notch signaling functions as a cell-fate switch between the endothelial and hematopoietic lineages
    Christina Y Lee
    McAllister Heart Institute, Department of Cell and Molecular Physiology, and Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Biol 19:1616-22. 2009
    ....
  4. pmc Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis
    Trista E North
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 447:1007-11. 2007
    ..The conserved role for PGE2 in the regulation of vertebrate HSC homeostasis indicates that modulation of the prostaglandin pathway may facilitate expansion of HSC number for therapeutic purposes...
  5. pmc Haematopoietic stem cells derive directly from aortic endothelium during development
    Julien Y Bertrand
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093 0380, USA
    Nature 464:108-11. 2010
    ..Finally, using a permanent lineage tracing strategy, we demonstrate that the HSCs generated from haemogenic endothelium are the lineal founders of the adult haematopoietic system...
  6. pmc Hematopoietic stem cell fate is established by the Notch-Runx pathway
    Caroline Erter Burns
    Stem Cell Program and Division of Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 19:2331-42. 2005
    ..These data define the Notch-Runx pathway as critical for the developmental specification of HSC fate and the subsequent homeostasis of HSC number, thus providing a mechanism for amplifying stem cells in vivo...
  7. ncbi Lmo2 and Scl/Tal1 convert non-axial mesoderm into haemangioblasts which differentiate into endothelial cells in the absence of Gata1
    Martin Gering
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Development 130:6187-99. 2003
    ..These results suggest that, in the absence of inducers of erythroid or myeloid haematopoiesis, Scl/Tal1-Lmo2-induced haemangioblasts differentiate into endothelial cells...
  8. ncbi Zebrafish scl functions independently in hematopoietic and endothelial development
    Kimberly A Dooley
    Division of Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute and Harvard Medical School, Howard Hughes Medical Institute, Boston, MA 02115, USA
    Dev Biol 277:522-36. 2005
    ..Surprisingly, in cloche, lmo2 was not induced in response to scl over-expression. Taken together, these findings support distinct roles for scl in hematopoietic and endothelial development, downstream of hemangioblast development...
  9. pmc Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation
    Shane P Herbert
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, Cardiovascular Research Institute, University of California, San Francisco, CA 94158, USA
    Science 326:294-8. 2009
    ..Thus, directional control of progenitor migration drives arterial-venous segregation and generation of separate parallel vessels from a single precursor vessel, a process essential for vascular development...
  10. doi Two functional microRNA-126s repress a novel target gene p21-activated kinase 1 to regulate vascular integrity in zebrafish
    Jun Zou
    Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, China
    Circ Res 108:201-9. 2011
    ..MicroRNAs (miRNAs) are key regulators of vascular development and diseases. The function and underlying mechanism of endothelial miRNAs have not been fully defined...
  11. pmc Functional analysis of zebrafish microfibril-associated glycoprotein-1 (Magp1) in vivo reveals roles for microfibrils in vascular development and function
    Eleanor Chen
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Blood 107:4364-74. 2006
    ..The essential role for MAGP1 in vascular morphogenesis and function also supports a wide range of clinical applications, including therapeutic targets in vascular disease and cardiovascular tissue engineering...
  12. doi Phospholipase C gamma-1 is required for granulocyte maturation in zebrafish
    Chang Bin Jing
    Key Laboratory of Stem Cell Biology, Laboratory of Development and Diseases, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, People s Republic of China
    Dev Biol 374:24-31. 2013
    ..These results suggested that plcg1 functioned specifically in zebrafish ICM granulopoiesis for the first time. Our studies suggest that specific pathways regulate the differentiation of the hematopoietic lineages...
  13. ncbi Vessel patterning in the embryo of the zebrafish: guidance by notochord
    B Fouquet
    Cardiovascular Research Center, Massachusetts General Hospital, Charlestown 02129, USA
    Dev Biol 183:37-48. 1997
    We have cloned the zebrafish homolog of the receptor tyrosine kinase flk-1 to provide us with a tool to study normal vascular pattern formation in the developing zebrafish embryo and to compare it to mutants in which vascular pattern is ..
  14. ncbi Cyclooxygenase-1 signaling is required for vascular tube formation during development
    Yong I Cha
    Department of Medicine, Cell and Developmental Biology and Cancer Biology, Vanderbilt University Medical Center and Vanderbilt Ingram Cancer Center, Vanderbilt University, 694 Preston Research Building, 2300 Pierce Ave, Nashville, TN 37232, USA
    Dev Biol 282:274-83. 2005
    ..Together, we provide the first evidence that COX-1 signaling is required for development of posterior mesoderm organs, specifically in the vascular tube formation and posterior nephric duct development...
  15. doi Fli1 acts at the top of the transcriptional network driving blood and endothelial development
    Feng Liu
    John Radcliffe Hospital, University of Oxford, OX3 9DS Oxford, United Kingdom
    Curr Biol 18:1234-40. 2008
    ..of Fli1 is sufficient to induce expression of key hemangioblast genes, such as Scl/Tal1, Lmo2, Gata2, Etsrp, and Flk1. Epistasis assays show that Fli1 expression is induced by Bmp signaling or Cloche, depending on the hemangioblast ..
  16. pmc The role of the ETS factor erg in zebrafish vasculogenesis
    Felix Ellett
    Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia
    Mech Dev 126:220-9. 2009
    ....
  17. pmc Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate
    Charles Williams
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37203, USA
    Dev Biol 341:196-204. 2010
    ..Collectively, these studies suggest that arterial endothelial cells are specified and formed via repressing venous cell fate at the lateral plate mesoderm by Hh signaling during vasculogenesis...
  18. ncbi Runx1 is required for zebrafish blood and vessel development and expression of a human RUNX1-CBF2T1 transgene advances a model for studies of leukemogenesis
    Maggie L Kalev-Zylinska
    Division of Molecular Medicine, The University of Auckland, Auckland, New Zealand
    Development 129:2015-30. 2002
    ..The phenotype obtained with transient expression of RUNX1-CBF2T1 validates the zebrafish as a model system to study t(8;21)-mediated leukemogenesis...
  19. ncbi Hedgehog signaling is required for adult blood stem cell formation in zebrafish embryos
    Martin Gering
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham, NG7 2UH, United Kingdom
    Dev Cell 8:389-400. 2005
    ..Furthermore, HSC and DA formation also share Vegf and Notch requirements, which further distinguishes them from primitive hematopoiesis and underlines their close relationship during vertebrate development...
  20. pmc Mutation in sodium-calcium exchanger 1 (NCX1) causes cardiac fibrillation in zebrafish
    Adam D Langenbacher
    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 102:17699-704. 2005
    ..These data signify the essential role of calcium homeostasis and NCX1h in establishing rhythmic contraction in the embryonic zebrafish heart...
  21. pmc Chemical modulation of receptor signaling inhibits regenerative angiogenesis in adult zebrafish
    Peter E Bayliss
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nat Chem Biol 2:265-73. 2006
    ..This study illustrates the utility of the adult zebrafish as a new model system for receptor signaling and chemical biology...
  22. pmc Duplicate VegfA genes and orthologues of the KDR receptor tyrosine kinase family mediate vascular development in the zebrafish
    Nathan Bahary
    Department of Medicine, University of Pittsburgh School of Medicine, PA15260, USA
    Blood 110:3627-36. 2007
    ..We have isolated a duplicated zebrafish VegfA locus, termed VegfAb, and a duplicate RTK locus with homology to KDR/FLK1 (named Kdrb)...
  23. doi Gfi1.1 regulates hematopoietic lineage differentiation during zebrafish embryogenesis
    Wei Wei
    Key Laboratory of Cell Proliferation and Differentiation, Center of Developmental Biology and Genetics, College of Life Sciences, Peking University, Ministry of Education, Beijing 100871, China
    Cell Res 18:677-85. 2008
    ..1 plays a critical role in regulating the balance of embryonic erythroid and myeloid lineage determination, and is also required for the differentiation of lymphocytes and granulocytes during zebrafish embryogenesis...
  24. ncbi The zebrafish gene cloche acts upstream of a flk-1 homologue to regulate endothelial cell differentiation
    W Liao
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0554, USA
    Development 124:381-9. 1997
    ..further the role of cloche in regulating endothelial cell differentiation, we have examined the expression of flk-1 and tie, two receptor tyrosine kinase genes expressed early and sequentially in the endothelial lineage...
  25. ncbi A role for notochord in axial vascular development revealed by analysis of phenotype and the expression of VEGR-2 in zebrafish flh and ntl mutant embryos
    L Sumoy
    Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
    Mech Dev 63:15-27. 1997
    ..Differences in the vascular phenotype between the two mutations correlated with the severity of their axial mesodermal defects. These observations support a role for the notochord in vasculogenesis...
  26. ncbi The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis
    M A Thompson
    Howard Hughes Medical Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Dev Biol 197:248-69. 1998
    ..The zebrafish homologs of lmo2, c-myb, fli1, flk1, and flt4 have been cloned and characterized in this study...
  27. pmc The SCL gene specifies haemangioblast development from early mesoderm
    M Gering
    University of Cambridge, Department of Haematology, MRC Centre, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 17:4029-45. 1998
    ..Our data also underline the striking similarities between the role of SCL in haematopoiesis/vasculogenesis and the function of other bHLH proteins in muscle and neural development...
  28. ncbi Hhex and scl function in parallel to regulate early endothelial and blood differentiation in zebrafish
    W Liao
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, University of California at San Francisco, San Francisco, CA 94143 0448, USA
    Development 127:4303-13. 2000
    ..leads to the premature and ectopic expression of early endothelial and blood differentiation genes such as fli1, flk1 and gata1, indicating that Hhex can positively regulate endothelial and blood differentiation...
  29. pmc Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant
    A Nasevicius
    Arnold and Mabel Beckman Center for Transposon Research, Department of Genetics, University of Minnesota, 6 160 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA
    Yeast 17:294-301. 2000
    ..Molecular analysis using the endothelial markers fli-1 and flk-1 at 1 day of development demonstrates a fundamental distinction between VEGF-A requirements for axial and ..
  30. pmc Vegfc is required for vascular development and endoderm morphogenesis in zebrafish
    Elke A Ober
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, University of California, San Francisco, California 94143 0448, USA
    EMBO Rep 5:78-84. 2004
    ....
  31. ncbi Transgenic zebrafish reveal stage-specific roles for Bmp signaling in ventral and posterior mesoderm development
    Ujwal J Pyati
    Department of Biochemistry, University of Washington, Seattle, WA 98195 7350, USA
    Development 132:2333-43. 2005
    ..We conclude that the role of Bmp signaling in the ventral and posterior mesoderm changes as gastrulation proceeds...
  32. ncbi Cellular and molecular analyses of vascular tube and lumen formation in zebrafish
    Suk Won Jin
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, Program in Developmental Biology, University of California San Francisco, 1550 Fourth Street, San Francisco, CA 94143, USA
    Development 132:5199-209. 2005
    ..To facilitate these studies, we generated a transgenic line where EGFP expression is controlled by the zebrafish flk1 promoter. We find that angioblasts migrate as individual cells to form a vascular cord at the midline...
  33. pmc Ets1-related protein is a key regulator of vasculogenesis in zebrafish
    Saulius Sumanas
    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, USA
    PLoS Biol 4:e10. 2006
    ..These results demonstrate that Etsrp is necessary and sufficient for the initiation of vasculogenesis...
  34. pmc Distinct genetic interactions between multiple Vegf receptors are required for development of different blood vessel types in zebrafish
    L D Covassin
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 103:6554-9. 2006
    ..Here, we describe a mutation in the zebrafish vegf receptor-2 homolog, kdra, which eliminates its kinase activity and leads to specific defects in artery development...
  35. ncbi Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish
    Kyeong Won Yoo
    Department of Biology, Chungnam National University, Daejeon 305 764, South Korea
    FEBS Lett 580:4409-16. 2006
    ..Taken together, we show that Snx5, a novel interacting partner of Mind bomb, may have an essential role for cell fate determination in early development...
  36. ncbi Mammalian tumor xenografts induce neovascularization in zebrafish embryos
    Stefania Nicoli
    Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy
    Cancer Res 67:2927-31. 2007
    ..and express the transcripts for the zebrafish orthologues of the early endothelial markers Fli-1, VEGF receptor-2 (VEGFR2/KDR), and VE-cadherin...
  37. pmc Interplay among Etsrp/ER71, Scl, and Alk8 signaling controls endothelial and myeloid cell formation
    Saulius Sumanas
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, USA
    Blood 111:4500-10. 2008
    ..We further demonstrate that the choice of endothelial versus myeloid fate depends on a combinatorial effect of etsrp, scl, and alk8 genes...
  38. pmc An acyltransferase controls the generation of hematopoietic and endothelial lineages in zebrafish
    Jing Wei Xiong
    The Nephrology Division, Massachusetts General Hospital East, Harvard Medical School, 149 13th St, Room 8216, Charlestown, MA 02129, USA
    Circ Res 102:1057-64. 2008
    ..Epistasis analysis supports that lycat acts upstream of scl and etsrp in zebrafish hemangioblast development. These data indicate that lycat is the earliest known player in the generation of both endothelial and hematopoietic lineages...
  39. doi Fibroblast growth factor 2-induced angiogenesis in zebrafish: the zebrafish yolk membrane (ZFYM) angiogenesis assay
    Stefania Nicoli
    General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia Medical School, Brescia, Italy
    J Cell Mol Med 13:2061-8. 2009
    ..are formed by proliferating cells that incorporate bromodeoxyuridine and express the endothelial cell markers vegfr2/kdr and fli1...
  40. pmc Rapid mutation of endogenous zebrafish genes using zinc finger nucleases made by Oligomerized Pool ENgineering (OPEN)
    Jonathan E Foley
    Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
    PLoS ONE 4:e4348. 2009
    ..The Consortium has previously used this new method (known as OPEN for Oligomerized Pool ENgineering) to generate high quality ZFN pairs that function in human and plant cells...
  41. doi The Integrator subunits function in hematopoiesis by modulating Smad/BMP signaling
    Shijie Tao
    Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore
    Development 136:2757-65. 2009
    ....
  42. pmc Chemokine signaling guides regional patterning of the first embryonic artery
    Arndt F Siekmann
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01602, USA
    Genes Dev 23:2272-7. 2009
    ..These studies reveal unexpected endothelial diversity within the aorta that is necessary to facilitate its regional patterning by local cues...
  43. pmc In vivo structure-activity relationship study of dorsomorphin analogues identifies selective VEGF and BMP inhibitors
    Jijun Hao
    Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    ACS Chem Biol 5:245-53. 2010
    ..has significant "off-target" effects against the VEGF (vascular endothelial growth factor) type-2 receptor (Flk1/KDR) and disrupts zebrafish angiogenesis...
  44. doi Blood stem cells emerge from aortic endothelium by a novel type of cell transition
    Karima Kissa
    Institut Pasteur, Unité Macrophages et Développement de l Immunité and CNRS URA2578, 25 rue du Dr Roux, F 75724 Paris Cedex 15, France
    Nature 464:112-5. 2010
    ....
  45. pmc Scl isoforms act downstream of etsrp to specify angioblasts and definitive hematopoietic stem cells
    Xi Ren
    Key Laboratory of Cell Proliferation and Differentiation, Center of Developmental Biology and Genetics, College of Life Sciences, Peking University, Ministry of Education, China
    Blood 115:5338-46. 2010
    ....
  46. doi von Hippel-Lindau tumor suppressor mutants faithfully model pathological hypoxia-driven angiogenesis and vascular retinopathies in zebrafish
    Ellen van Rooijen
    Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Utrecht, The Netherlands
    Dis Model Mech 3:343-53. 2010
    ..increased expression of the duplicated VEGFA orthologs vegfaa and vegfab, and of vegfb and its receptors flt1, kdr and kdr-like, indicating increased vascular endothelial growth factor (Vegf) signaling in vhl mutants...
  47. pmc Assembly and patterning of the vascular network of the vertebrate hindbrain
    Misato Fujita
    Laboratory of Molecular Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Development 138:1705-15. 2011
    ..Knockdown of either cxcl12b or cxcr4a results in defects in basilar artery formation, showing that the assembly and patterning of this crucial artery depends on chemokine signaling...
  48. pmc Semaphorin-PlexinD1 signaling limits angiogenic potential via the VEGF decoy receptor sFlt1
    Tomasz Zygmunt
    Department of Cell Biology, Helen L and Martin S Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Dev Cell 21:301-14. 2011
    ..Hence, Sema-PlxnD1 signaling regulates distinct but related aspects of angiogenesis: the spatial allocation of angiogenic capacity within a primary vessel and sprout guidance...
  49. pmc ApoB-containing lipoproteins regulate angiogenesis by modulating expression of VEGF receptor 1
    Inbal Avraham-Davidi
    Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
    Nat Med 18:967-73. 2012
    ..These findings may open new avenues for the treatment of lipoprotein-related vascular disorders...
  50. pmc Arterial and venous progenitors of the major axial vessels originate at distinct locations
    Vikram Kohli
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Dev Cell 25:196-206. 2013
    ..We propose a revised model of arterial-venous differentiation that explains how angioblasts choose between an arterial and venous fate...
  51. doi In vivo analysis reveals a highly stereotypic morphogenetic pathway of vascular anastomosis
    Anna Lenard
    Biozentrum der Universitat Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Dev Cell 25:492-506. 2013
    ..Vascular endothelial-cadherin plays an important role early in the anastomosis process and is required for filopodial tip cell interactions and efficient formation of a single contact site...
  52. pmc SCL/Tal-1 transcription factor acts downstream of cloche to specify hematopoietic and vascular progenitors in zebrafish
    E C Liao
    Division of Hematology Oncology, Children s Hospital, Department of Pediatrics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 12:621-6. 1998
    ..Forced expression of SCL in clo embryos rescues the blood and vascular defects, suggesting that SCL acts downstream of clo to specify hematopoietic and vascular differentiation...
  53. ncbi Ontogeny and behaviour of early macrophages in the zebrafish embryo
    P Herbomel
    Unité de Biologie moléculaire du Développement, URA1947 du CNRS, Departement de Biologie Moleculaire, Institut Pasteur, 75724 Paris Cedex 15, France
    Development 126:3735-45. 1999
    ....
  54. ncbi The role of vascular endothelial growth factor (VEGF) in vasculogenesis, angiogenesis, and hematopoiesis in zebrafish development
    D Liang
    Department of Biological Sciences, National University of Singapore, Singapore 119260
    Mech Dev 108:29-43. 2001
    ..Simultaneous overexpression of both isoforms in the embryo results in increased production of flk1, tie1, scl, and gata1 transcripts, indicating a stimulation of both endothelial and hematopoietic lineages...
  55. ncbi Gridlock signalling pathway fashions the first embryonic artery
    T P Zhong
    Cardiovascular Research Center, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Charlestown 02129, USA
    Nature 414:216-20. 2001
    ..Thus, a notch-grl pathway controls assembly of the first embryonic artery, apparently by adjudicating an arterial versus venous cell fate decision...
  56. ncbi Dissection of angiogenic signaling in zebrafish using a chemical genetic approach
    Joanne Chan
    Department of Cancer Biology, Dana Farber Cancer Institute and the Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Cancer Cell 1:257-67. 2002
    ..This approach allowed us to examine the effects of blood flow and the role of endothelial signals in organogenesis...
  57. ncbi sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Cell 3:127-36. 2002
    ..These studies reveal a complex signaling cascade responsible for establishing arterial cell fate and suggest differential effects of Vegf on developing endothelial cells...
  58. ncbi Analysis of a zebrafish VEGF receptor mutant reveals specific disruption of angiogenesis
    Hinrich Habeck
    Artemis Pharmaceuticals Exelixis Deutschland GmbH, 72076, Tubingen, Germany
    Curr Biol 12:1405-12. 2002
    ..Targeted disruption of vascular endothelial growth factor (VEGF) or its receptor kdr (flk1, VEGFR2) in mouse embryos results in a severe reduction of all blood vessels, while the complete loss of flt1 (VEGFR1) ..
  59. pmc phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development
    Nathan D Lawson
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:1346-51. 2003
    ..Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system...
  60. ncbi Syndecan-2 is essential for angiogenic sprouting during zebrafish development
    Eleanor Chen
    Arnold and Mabel Beckman Center for Transposon Research, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Blood 103:1710-9. 2004
    ..Syndecan-2 as a novel angiogenic factor is a potential candidate for use in the development of angiogenesis-based therapies...
  61. ncbi The endothelial-cell-derived secreted factor Egfl7 regulates vascular tube formation
    Leon H Parker
    Molecular Biology Department, Genentech Inc, South San Francisco, California 94080, USA
    Nature 428:754-8. 2004
    ..This process fails to take place in Egfl7 knockdown embryos, leading to the failure of vascular tube formation. Our study defines a regulator that controls a specific and important step in vasculogenesis...
  62. ncbi Zebrafish genetics and formation of embryonic vasculature
    Tao P Zhong
    Departments of Medicine and Cell and Developmental Biology Vanderbilt University School of Medicine Nashville, Tennessee 37232, USA
    Curr Top Dev Biol 71:53-81. 2005
    ..These studies highlight the unique utilities and benefits of the zebrafish system for studying development of embryonic blood vessels...
  63. ncbi Notch signaling regulates midline cell specification and proliferation in zebrafish
    Andrew J Latimer
    Department of Biological Sciences, Vanderbilt University, U7211 BSB MRBIII, 465 21st Avenue South, Nashville, TN 37232, USA
    Dev Biol 298:392-402. 2006
    ..Together, our results indicate that Notch signaling regulates allocation of appropriate numbers of different midline cells by different mechanisms...
  64. pmc Combinatorial function of ETS transcription factors in the developing vasculature
    Van N Pham
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development NIH, Building 6B, Room 309, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 303:772-83. 2007
    ..Our results demonstrate that combinatorial ETS factor function is essential for early endothelial specification and differentiation...
  65. pmc FoxH1 negatively modulates flk1 gene expression and vascular formation in zebrafish
    Jayoung Choi
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA
    Dev Biol 304:735-44. 2007
    b>Flk1 is the major receptor for VEGF on endothelial cells. During embryogenesis, flk1 is required for both vasculogenesis and angiogenesis and abnormally elevated flk1 expression is often associated with pathological conditions in adults...
  66. pmc A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos
    Suk Won Jin
    Department of Biochemistry and Biophysics, Genetics and Human Genetics, and Cardiovascular Research Institute, University of California San Francisco, CA 94158, USA
    Dev Biol 307:29-42. 2007
    ..All putative mutants were then crossed into the Tg(flk1:EGFP)(s843) transgenic background to facilitate detailed examination of endothelial cells in live and fixed embryos...
  67. ncbi Sox18 and Sox7 play redundant roles in vascular development
    Solei Cermenati
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Via Celoria 26, Milan, Italy
    Blood 111:2657-66. 2008
    ..Our data suggest that a defect in arteriovenous identity could be responsible for the formation of telangiectases in patients with HLT...
  68. doi Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo
    Yannick Blum
    Biozentrum der Universitat Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Dev Biol 316:312-22. 2008
    ....
  69. pmc Foxn4 directly regulates tbx2b expression and atrioventricular canal formation
    Neil C Chi
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, University of California, San Francisco, San Francisco, California 94158, USA
    Genes Dev 22:734-9. 2008
    ..sli/foxn4 is expressed in the AV canal, and its encoded product binds to a highly conserved tbx2 enhancer domain that contains Foxn4- and T-box-binding sites, both necessary to regulate tbx2b expression in the AV canal...
  70. pmc A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development
    L D Covassin
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, Room 617, 364 Plantation Street, Worcester, MA 01605, USA
    Dev Biol 329:212-26. 2009
    ..with different mutations within a functional zebrafish Vegf receptor-2 ortholog (referred to as kdr-like, kdrl) revealed surprisingly varied effects on vascular development...
  71. pmc Hematopoietic stem cell development is dependent on blood flow
    Trista E North
    Stem Cell Program and Hematology Oncology, Children s Hospital, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 137:736-48. 2009
    ..This work links blood flow to AGM hematopoiesis and identifies NO as a conserved downstream regulator of HSC development...
  72. pmc Disruption of angiogenesis and tumor growth with an orally active drug that stabilizes the inactive state of PDGFRbeta/B-RAF
    Eric A Murphy
    Department of Pathology, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 107:4299-304. 2010
    ..Our lead compound was selected as an orally active molecule with favorable pharmacokinetic properties which demonstrated target inhibition in vivo leading to suppression of murine orthotopic tumors in both the kidney and pancreas...
  73. pmc Calycosin promotes angiogenesis involving estrogen receptor and mitogen-activated protein kinase (MAPK) signaling pathway in zebrafish and HUVEC
    Jing Yan Tang
    Institute of Chinese Medical Sciences, University of Macau, Macao, China
    PLoS ONE 5:e11822. 2010
    ..The present study investigates the angiogenic effects of calycosin, a major isoflavonoid isolated from Radix Astragali, in vitro and in vivo...
  74. doi Gicerin/Cd146 is involved in zebrafish cardiovascular development and tumor angiogenesis
    Ju Hoon So
    Department of Biology and GRAST, Chungnam National University, Daejeon, Korea
    Genes Cells 15:1099-110. 2010
    ..Thus, knock-down of gicerin might have an important implication in controlling tumor angiogenesis...
  75. pmc Arterial-venous network formation during brain vascularization involves hemodynamic regulation of chemokine signaling
    Jeroen Bussmann
    Max Planck Institute for Molecular Biomedicine, Muenster, Germany
    Development 138:1717-26. 2011
    ....
  76. doi Mef2cb regulates late myocardial cell addition from a second heart field-like population of progenitors in zebrafish
    Savo Lazic
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    Dev Biol 354:123-33. 2011
    ..Further, as congenital heart disease is often associated with defects in second heart field development, the embryological and genetic advantages of the zebrafish model can be applied to study the vertebrate second heart field...
  77. pmc Distinct signalling pathways regulate sprouting angiogenesis from the dorsal aorta and the axial vein
    David M Wiley
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 13:686-92. 2011
    ....
  78. doi Transcriptional profiling of angiogenesis activities of calycosin in zebrafish
    Shang Li
    Institute of Chinese Medical Sciences, University of Macau, Av Padre Tomas Pereira, Taipa, Macau, China
    Mol Biosyst 7:3112-21. 2011
    ..The data have elucidated the connection between morphological observations and genomic evidence, indicating the potential roles of several key signaling pathways in angiogenesis...
  79. doi Hedgehog signaling is required for differentiation of endocardial progenitors in zebrafish
    Kuan Shen Wong
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, USA
    Dev Biol 361:377-91. 2012
    ..Our results argue that Hh provides a critical signal to induce the specification and differentiation of endocardial progenitors...
  80. doi In vitro and in vivo structure and activity relationship analysis of polymethoxylated flavonoids: identifying sinensetin as a novel antiangiogenesis agent
    In Kei Lam
    Institute of Chinese Medical Sciences, University of Macau, Avenue Padre Tomás Pereira S J, Taipa, Macao SAR, PR China
    Mol Nutr Food Res 56:945-56. 2012
    ..These compounds have potential for anticarcinogenesis, antitumor, and cardiovascular protective activity, but the effect on angiogenesis has not been well studied...
  81. pmc Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation
    Yaniv Hinits
    Randall Division of Cell and Molecular Biophysics, New Hunt s House, Guy s Campus, King s College London, UK
    Dev Biol 369:199-210. 2012
    ..Mef2cb single mutants have a functional heart and are viable adults. Our results show that the key role of Mef2c in myocardial differentiation is conserved throughout the vertebrate heart...
  82. pmc VEGF-PLCgamma1 pathway controls cardiac contractility in the embryonic heart
    Wolfgang Rottbauer
    Department of Medicine III, University of Heidelberg, D 69120 Heidelberg, Germany
    Genes Dev 19:1624-34. 2005
    ..Thus, the muscle of the heart uses the VEGF-PLCgamma1 cascade to control the strength of the heart beat. We speculate that this paracrine system may contribute to normal and pathological regulation of cardiac contractility...
  83. ncbi Genetic and cellular analyses of zebrafish atrioventricular cushion and valve development
    Dimitris Beis
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    Development 132:4193-204. 2005
    ..This collection of mutants provides a unique set of tools to further our understanding of the genetic basis of cell behavior and differentiation during AV valve development...
  84. ncbi alyron, an insertional mutation affecting early neural crest development in zebrafish
    C J Cretekos
    Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, Utah, 84112, USA
    Dev Biol 210:322-38. 1999
    ....
  85. ncbi Specification of hematopoietic and vascular development by the bHLH transcription factor SCL without direct DNA binding
    C Porcher
    Division of Hematology and Oncology, Children s Hospital and Dana Farber Cancer Institute, Department of Pediatrics, Harvard Medical School and Howard Hughes Medical Institute, Boston, MA 02115, USA
    Development 126:4603-15. 1999
    ....
  86. ncbi Sequence and embryonic expression of deltaC in the zebrafish
    L Smithers
    Vertebrate Development Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London, UK
    Mech Dev 90:119-23. 2000
    ..There is expression also in notochord, blood vessels and pronephros...
  87. ncbi Hematopoietic perturbation in zebrafish expressing a tel-jak2a fusion
    Sara M N Onnebo
    Centre for Cellular and Molecular Biology, School of Biological and Chemical Sciences, Deakin University, Burwood, Victoria, Australia
    Exp Hematol 33:182-8. 2005
    ..Such fusions can mediate factor-independent growth of hematopoietic cell lines and induction of malignancy in mouse models...
  88. ncbi Ethanol disrupts the formation of hypochord and dorsal aorta during the development of embryonic zebrafish
    Linxi Qian
    Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical School of Fudan University, Shanghai 200032, China
    Sci China C Life Sci 48:608-15. 2005
    ..By employing in situ hybridization with endothelial biomarker (Flk-1), we revealed that ethanol disrupts the establishment of trunk axial vasculature, but has no effect on cranial ..
  89. ncbi Endothelium is required for the promotion of interrenal morphogenetic movement during early zebrafish development
    Yi Wen Liu
    Department of Life Science, Tunghai University, Taichung 40704, Taiwan R O C
    Dev Biol 297:44-58. 2006
    ..We thus conclude that endothelial signaling is involved in the morphogenetic movement of early interrenal tissue...
  90. pmc Biphasic role for Wnt/beta-catenin signaling in cardiac specification in zebrafish and embryonic stem cells
    Shuichi Ueno
    Department of Pathology, Center for Cardiovascular Biology, University of Washington School of Medicine, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 104:9685-90. 2007
    ..Thus, Wnt/beta-catenin signaling promotes cardiac differentiation at early developmental stages and inhibits it later. Control of this pathway may promote derivation of cardiomyocytes for basic research and cell therapy applications...
  91. ncbi Endocardium is necessary for cardiomyocyte movement during heart tube assembly
    Nathalia Glickman Holtzman
    Developmental Genetics Program and Department of Cell Biology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Development 134:2379-86. 2007
    ..Together, our data reveal a dynamic cellular mechanism by which tissue interactions establish organ architecture...
  92. pmc Infectious spleen and kidney necrosis virus ORF48R functions as a new viral vascular endothelial growth factor
    Zi Liang Wang
    State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat Sen Zhongshan University, 135 Xingang Road West, Guangzhou 510275, People s Republic of China
    J Virol 82:4371-83. 2008
    ..ISKNV ORF48R is also able to stimulate a striking expression of flk1 in the zebrafish dorsal aorta and the axial vein...
  93. pmc odd skipped related1 reveals a novel role for endoderm in regulating kidney versus vascular cell fate
    Sudha P Mudumana
    Nephrology Division, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Development 135:3355-67. 2008
    ....
  94. pmc A non-canonical function of zebrafish telomerase reverse transcriptase is required for developmental hematopoiesis
    Shintaro Imamura
    Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, USA
    PLoS ONE 3:e3364. 2008
    ..The data also provide insights into a non-canonical pathway by which TERT functions to modulate specification of hematopoietic stem/progenitor cells during vertebrate development. (276 words)...
  95. doi Critical role of connexin43 in zebrafish late primitive and definitive hematopoiesis
    Qiu Jiang
    Department of Biochemistry and Molecular Biology, Shanghai Medical College and Key Laboratory of Molecular Medicine, Ministry of Education, Children s Hospital, Fudan University, 200032, Shanghai, People s Republic of China
    Fish Physiol Biochem 36:945-51. 2010
    ..Whole-mount in situ hybridization analysis revealed that the hemangioblastic marker flk-1 and the primitive hematopoietic markers lmo2 and scl are basically maintained at normal levels in cx43 morphant ..
  96. pmc Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity
    Anjali Verma
    Department of Pediatrics and Developmental Vascular Biology Program, Medical College of Wisconsin and Children s Research Institute, Milwaukee, WI 53226, USA
    Blood 115:4614-22. 2010
    ..ECSCR localized to actin-rich membrane protrusions, colocalizing with kinase insert domain protein receptor (KDR)/VEGF receptor 2 in these regions...
  97. pmc Joint dynamic imaging of morphogenesis and function in the developing heart
    Jungho Ohn
    Electrical and Computer Engineering University of California Santa Barbara, CA USA
    Organogenesis 5:166-73. 2009
    ..We present results obtained in the developing zebrafish heart. Image analysis of the acquired data yielded blood flow velocity maps and made it possible to follow the relative movement of individual cells over several hours...
  98. doi Live imaging of Runx1 expression in the dorsal aorta tracks the emergence of blood progenitors from endothelial cells
    Enid Yi Ni Lam
    Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland, Auckland, New Zealand
    Blood 116:909-14. 2010
    ..This study captures the earliest events of the transition of endothelial cells to a hemogenic endothelium and demonstrates that embryonic hematopoietic progenitors directly differentiate from endothelial cells within a living organism...
  99. doi Zebrafish notch signalling pathway mutants exhibit trunk vessel patterning anomalies that are secondary to somite misregulation
    Christina Therapontos
    NHLI, Faculty of Medicine, Imperial College London, London, United Kingdom
    Dev Dyn 239:2761-8. 2010
    ..Ectopic filopodia are not due to loss of dll4. Somite expression of known vascular guidance cues, efnb2, sema3a2, and plexinD1 are disrupted, suggesting that the ISV vascular phenotype is due to disruption of these cues...
  100. doi Gα13 is closely related to hematopoiesis in zebrafish
    Ding Ye
    Laboratory of Molecular Genetics and Developmental Biology, College of Life Sciences, Wuhan University, Department of Gynaecology, South Central Hospital, Wuhan, 430072, People s Republic of China
    Mol Biol Rep 38:2685-94. 2011
    ..The Gα13b morphants don't show complete loss of expression of gata1, pu.1 or flk until 35 hpf suggests that Gα13b is closely related to the development of hematopoietic cells...
  101. pmc Latent TGF-β binding protein 3 identifies a second heart field in zebrafish
    Yong Zhou
    Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Nature 474:645-8. 2011
    ..Taken together, our findings uncover a requirement for ltbp3-TGF-β signalling during zebrafish SHF development, a process that serves to enlarge the single ventricular chamber in this species...

Research Grants6

  1. Cloning and Function of Cloche in Vascular Development
    Jing Wei Xiong; Fiscal Year: 2006
    ..We have identified cloche mutants in large-scale mutagenesis screens of the zebrafish genome. cloche lacks all flk-l-expressing cells, and fails to form both vessels and blood...
  2. Identifying Vegf target genes during artery development
    Nathan Lawson; Fiscal Year: 2005
    ....
  3. Genetic Analysis of Maternal Factors in Embryonic Devel*
    Mary Mullins; Fiscal Year: 2005
    ..These studies will be relevant to the nature of human sterilities, birth defects, and human inherited disorders. ..
  4. DORSAL-VENTRAL PATTERN FORMATION IN THE ZEBRAFISH EMBRYO
    Mary Mullins; Fiscal Year: 2007
    ..Thus it is critical to identify and study all the genetic players in this process. ..
  5. Role of Plcg1 in Vegf signaling
    Nathan Lawson; Fiscal Year: 2009
    ..Since these signaling mechanisms are evolutionary conserved, the molecules we identify in this proposal represent possible targets for clinical manipulation of pathological blood vessel formation. ..
  6. Genetic analysis of ovary development and oogenesis
    Mary Mullins; Fiscal Year: 2009
    ..Identification of the mutant zebrafish genes will provide candidate genes responsible for the human defects, which could ultimately lead to genetic testing or therapeutic intervention. ..