VP35

Summary

Gene Symbol: VP35
Description: polymerase complex protein
Species: Zaire ebolavirus

Top Publications

  1. pmc The Ebola virus VP35 protein functions as a type I IFN antagonist
    C F Basler
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 97:12289-94. 2000
  2. pmc Reverse genetic generation of recombinant Zaire Ebola viruses containing disrupted IRF-3 inhibitory domains results in attenuated virus growth in vitro and higher levels of IRF-3 activation without inhibiting viral transcription or replication
    Amy L Hartman
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 14, Atlanta, GA 30329, USA
    J Virol 80:6430-40. 2006
  3. ncbi The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein
    Yue Huang
    Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 10:307-16. 2002
  4. pmc Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling
    Washington B Cardenas
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 80:5168-78. 2006
  5. pmc VP35 knockdown inhibits Ebola virus amplification and protects against lethal infection in mice
    Sven Enterlein
    Department of Virology, Philipps University Marburg, Hans Meerwein str 3, 35043 Marburg, Germany
    Antimicrob Agents Chemother 50:984-93. 2006
  6. doi Identification of novel VP35 inhibitors: Virtual screening driven new scaffolds
    Ji Xia Ren
    College of Life Science, Liaocheng University, Liaocheng 252059, People s Republic of China Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, People s Republic of China
    Biomed Pharmacother 84:199-207. 2016
  7. pmc Differential Regulation of Interferon Responses by Ebola and Marburg Virus VP35 Proteins
    Megan R Edwards
    Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell Rep 14:1632-40. 2016
  8. doi In silico study of VP35 inhibitors: from computational alanine scanning to essential dynamics
    F Dapiaggi
    Dipartimento di Chimica, Universita degli Studi di Milano, Via Golgi 19, 20133 Milano, Italy
    Mol Biosyst 11:2152-7. 2015
  9. pmc Different Temporal Effects of Ebola Virus VP35 and VP24 Proteins on Global Gene Expression in Human Dendritic Cells
    Philipp A Ilinykh
    Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA Galveston National Laboratory, University of Texas Medical Branch at Galveston, Galveston, Texas, USA
    J Virol 89:7567-83. 2015
  10. pmc Ebola virus VP35 interaction with dynein LC8 regulates viral RNA synthesis
    Priya Luthra
    Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
    J Virol 89:5148-53. 2015

Research Grants

  1. EBOLA VIRUS INTERFERON-ANTAGONISTS AND VIRULENCE
    CHRISTOPHER BASLER; Fiscal Year: 2003
  2. Lassa and Nipah Virus Interferon-Antagonists
    CHRISTOPHER BASLER; Fiscal Year: 2003

Scientific Experts

  • S Pieraccini
  • CHRISTOPHER BASLER
  • John C Kash
  • Reed F Johnson
  • Heinz Feldmann
  • Daisy W Leung
  • Gaya K Amarasinghe
  • Kathleen C Prins
  • Priya Luthra
  • Benjamin Yen
  • Washington B Cardenas
  • Amy L Hartman
  • V E Volchkov
  • Elke Mühlberger
  • Parameshwaran Ramanan
  • Richard B Honzatko
  • Sven Enterlein
  • Ji Xia Ren
  • Megan R Edwards
  • St Patrick Reid
  • F Dapiaggi
  • Philipp A Ilinykh
  • Thomas W Geisbert
  • Chad E Mire
  • Gai Liu
  • Kelly L Warfield
  • Reed S Shabman
  • Martina Trunschke
  • Osvaldo Martinez
  • Allison Groseth
  • Yali Zhu
  • Luca Zinzula
  • Steven Theriault
  • Giulia Fabozzi
  • Lawrence W Leung
  • Huali Jin
  • Jay C Nix
  • Nathaniel D Ginder
  • Michael Schumann
  • Joost Haasnoot
  • E L Subbotina
  • Shinji Watanabe
  • David A Stein
  • Sina Bavari
  • Patrick L Iversen
  • Dana L Swenson
  • Stuart T Nichol
  • Yoshihiro Kawaoka
  • Jonathan S Towner
  • V A Volchkova
  • H D Klenk
  • Suhas Sureshchandra
  • Ilhem Messaoudi
  • Li Ke Liu
  • Xue Song Cao
  • Hui Zhang
  • Jens Modrof
  • Rui Tao Zhang
  • Yong Xie
  • Steven G Widen
  • Ndongala M Lubaki
  • Alexander Bukreyev
  • A A Chepurnov
  • Lynnsey A Renn
  • M Sironi
  • Terence C Theisen
  • David S Jordan
  • Thomas G Wood
  • Ronald L Rabin
  • Yue Huang
  • Lubbertus C F Mulder
  • Dominik Conrad
  • Carla Weisend
  • Yoshimi Tsuda
  • Hideki Ebihara
  • Judith Olejnik
  • Kristina Brauburger
  • Kenneth J Buckley
  • David M Bisaro
  • A Takada
  • Jamie N Jackel
  • Nil Celebi Cherukuri
  • Deborah S Parris
  • Zetang Wu
  • Monica Crary
  • Daniela Pala
  • Francesca Esposito
  • Enzo Tramontano
  • Christopher S Nabel
  • Charalampos Valmas

Detail Information

Publications47

  1. pmc The Ebola virus VP35 protein functions as a type I IFN antagonist
    C F Basler
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 97:12289-94. 2000
    ..Using this assay, we have identified an Ebola virus-encoded inhibitor of the type I IFN response, the Ebola virus VP35 protein...
  2. pmc Reverse genetic generation of recombinant Zaire Ebola viruses containing disrupted IRF-3 inhibitory domains results in attenuated virus growth in vitro and higher levels of IRF-3 activation without inhibiting viral transcription or replication
    Amy L Hartman
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 14, Atlanta, GA 30329, USA
    J Virol 80:6430-40. 2006
    The VP35 protein of Zaire Ebola virus is an essential component of the viral RNA polymerase complex and also functions to antagonize the cellular type I interferon (IFN) response by blocking activation of the transcription factor IRF-3...
  3. ncbi The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein
    Yue Huang
    Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 10:307-16. 2002
    ..Expression of the nucleoprotein (NP) and virion-associated proteins VP35 and VP24 led to spontaneous assembly of nucleocapsids in transfected 293T cells by transmission electron microscopy...
  4. pmc Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling
    Washington B Cardenas
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 80:5168-78. 2006
    The Ebola virus (EBOV) VP35 protein blocks the virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF-3), a transcription factor critical for the induction of alpha/beta interferon (IFN-alpha/beta) expression...
  5. pmc VP35 knockdown inhibits Ebola virus amplification and protects against lethal infection in mice
    Sven Enterlein
    Department of Virology, Philipps University Marburg, Hans Meerwein str 3, 35043 Marburg, Germany
    Antimicrob Agents Chemother 50:984-93. 2006
    ..A 22-mer P-PMO designed to base pair with the translation start site region of EBOV VP35 positive-sense RNA generated sequence-specific and time- and dose-dependent inhibition of EBOV amplification in ..
  6. doi Identification of novel VP35 inhibitors: Virtual screening driven new scaffolds
    Ji Xia Ren
    College of Life Science, Liaocheng University, Liaocheng 252059, People s Republic of China Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, People s Republic of China
    Biomed Pharmacother 84:199-207. 2016
    ..The Ebola virus Viral Protein 35 (VP35) is a multifunctional protein which is critical for virus replication and infection, and it is considered as a ..
  7. pmc Differential Regulation of Interferon Responses by Ebola and Marburg Virus VP35 Proteins
    Megan R Edwards
    Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell Rep 14:1632-40. 2016
    ..Ebola (EBOV) and Marburg (MARV) viruses each encode a VP35 protein that suppresses RIG-I-like receptor signaling and interferon-α/β (IFN-α/β) production by several ..
  8. doi In silico study of VP35 inhibitors: from computational alanine scanning to essential dynamics
    F Dapiaggi
    Dipartimento di Chimica, Universita degli Studi di Milano, Via Golgi 19, 20133 Milano, Italy
    Mol Biosyst 11:2152-7. 2015
    ..The Ebola virus Viral Protein 35 (VP35) carries out multiple functions necessary for virus replication and infection, in particular interfering with (IFN)-..
  9. pmc Different Temporal Effects of Ebola Virus VP35 and VP24 Proteins on Global Gene Expression in Human Dendritic Cells
    Philipp A Ilinykh
    Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA Galveston National Laboratory, University of Texas Medical Branch at Galveston, Galveston, Texas, USA
    J Virol 89:7567-83. 2015
    ..demonstrated that DC maturation is unblocked by disabling the innate response antagonizing domains (IRADs) in EBOV VP35 and VP24 by the mutations R312A and K142A, respectively...
  10. pmc Ebola virus VP35 interaction with dynein LC8 regulates viral RNA synthesis
    Priya Luthra
    Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
    J Virol 89:5148-53. 2015
    Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor...
  11. pmc Molecular basis for ebolavirus VP35 suppression of human dendritic cell maturation
    Benjamin Yen
    Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
    J Virol 88:12500-10. 2014
    Zaire ebolavirus (EBOV) VP35 is a double-stranded RNA (dsRNA)-binding protein that inhibits RIG-I signaling and alpha/beta interferon (IFN-α/β) responses by both dsRNA-binding-dependent and -independent mechanisms...
  12. pmc Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome
    Priya Luthra
    Department of Microbiology, Icahn School of Medicine at Mount Sinai School, New York, NY 10029, USA
    Cell Host Microbe 14:74-84. 2013
    ..Here, we show that EBOV VP35 inhibits PACT-induced RIG-I ATPase activity in a dose-dependent manner...
  13. pmc The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3
    Christopher F Basler
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 77:7945-56. 2003
    The Ebola virus VP35 protein was previously found to act as an interferon (IFN) antagonist which could complement growth of influenza delNS1 virus, a mutant influenza virus lacking the influenza virus IFN antagonist protein, NS1...
  14. pmc Characterization of the RNA silencing suppression activity of the Ebola virus VP35 protein in plants and mammalian cells
    Yali Zhu
    Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University, Columbus, Ohio, USA
    J Virol 86:3038-49. 2012
    ..EBOV VP35 is a virulence factor that blocks innate antiviral host responses, including the induction of and response to alpha/..
  15. pmc Ebolavirus VP35 suppresses IFN production from conventional but not plasmacytoid dendritic cells
    Lawrence W Leung
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Immunol Cell Biol 89:792-802. 2011
    ..The VP35 protein impairs RIG-I-like receptor-dependent signaling to inhibit IFN production, and this function has been ..
  16. doi dsRNA binding characterization of full length recombinant wild type and mutants Zaire ebolavirus VP35
    Luca Zinzula
    Department of Life and Environmental Sciences, University of Cagliari, Cittadella di Monserrato SS554, 09042 Monserrato, Cagliari, Italy
    Antiviral Res 93:354-63. 2012
    The Ebola viruses (EBOVs) VP35 protein is a multifunctional major virulence factor involved in EBOVs replication and evasion of the host immune system...
  17. pmc The L-VP35 and L-L interaction domains reside in the amino terminus of the Ebola virus L protein and are potential targets for antivirals
    Martina Trunschke
    Department of Virology, Philipps University of Marburg, Hans Meerwein Strβe 2, 35043 Marburg, Germany
    Virology 441:135-45. 2013
    ..RNA polymerase (RdRp) complex consists of the catalytic subunit of the polymerase, L, and its cofactor VP35. Using immunofluorescence analysis and coimmunoprecipitation assays, we mapped the VP35 binding site on L...
  18. pmc Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway
    Giulia Fabozzi
    Biodefense Research Section, Vaccine Research Center, National Institutes of Health, Vaccine Research Center, 40 Convent Drive, Bldg 40 2509, Bethesda, MD 20892, USA
    J Virol 85:2512-23. 2011
    ..In addition to viral protein 35 (VP35), we found that VP30 and VP40 independently act as SRSs. Here, we present the molecular mechanisms of VP30 and VP35...
  19. pmc Basic residues within the ebolavirus VP35 protein are required for its viral polymerase cofactor function
    Kathleen C Prins
    Department of Microbiology, Mount Sinai School of Medicine, Box 1124, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 84:10581-91. 2010
    The ebolavirus (EBOV) VP35 protein binds to double-stranded RNA (dsRNA), inhibits host alpha/beta interferon (IFN-α/β) production, and is an essential component of the viral polymerase complex...
  20. pmc Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35
    Daisy W Leung
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA
    Nat Struct Mol Biol 17:165-72. 2010
    Ebola viral protein 35 (VP35), encoded by the highly pathogenic Ebola virus, facilitates host immune evasion by antagonizing antiviral signaling pathways, including those initiated by RIG-I-like receptors...
  21. pmc Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology
    Reed F Johnson
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce St, Philadelphia, PA 19104, USA
    Virol J 3:31. 2006
    Recently we described a role for Ebola virus proteins, NP, GP, and VP35 in enhancement of VP40 VLP budding...
  22. pmc Mutations abrogating VP35 interaction with double-stranded RNA render Ebola virus avirulent in guinea pigs
    Kathleen C Prins
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Virol 84:3004-15. 2010
    Ebola virus (EBOV) protein VP35 is a double-stranded RNA (dsRNA) binding inhibitor of host interferon (IFN)-alpha/beta responses that also functions as a viral polymerase cofactor...
  23. pmc The VP35 protein of Ebola virus impairs dendritic cell maturation induced by virus and lipopolysaccharide
    Huali Jin
    Department of Microbiology and Immunology, College of Medicine, University of Illinois, Chicago, IL 60612, USA
    J Gen Virol 91:352-61. 2010
    ..However, the underlying mechanism remains unclear. In this study, we report that Ebola virus VP35 impairs the maturation of mouse DCs...
  24. pmc Ebola virus VP35 antagonizes PKR activity through its C-terminal interferon inhibitory domain
    Michael Schumann
    Department of Virology, Philipps University Marburg, 35043 Marburg, Germany
    J Virol 83:8993-7. 2009
    Ebola virus VP35 contains a C-terminal cluster of basic amino acids required for double-stranded RNA (dsRNA) binding and inhibition of interferon regulatory factor 3 (IRF3)...
  25. pmc Expression, purification, crystallization and preliminary X-ray studies of the Ebola VP35 interferon inhibitory domain
    Daisy W Leung
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:163-5. 2009
    Ebola VP35 is a multifunctional protein that is important for host immune suppression and pathogenesis. VP35 contains an N-terminal oligomerization domain and a C-terminal interferon inhibitory domain (IID)...
  26. pmc Ebola virus protein VP35 impairs the function of interferon regulatory factor-activating kinases IKKepsilon and TBK-1
    Kathleen C Prins
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Virol 83:3069-77. 2009
    The Ebola virus (EBOV) VP35 protein antagonizes the early antiviral alpha/beta interferon (IFN-alpha/beta) response...
  27. pmc Structure of the Ebola VP35 interferon inhibitory domain
    Daisy W Leung
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 106:411-6. 2009
    ..The Ebola VP35 protein is multifunctional, acting as a component of the viral RNA polymerase complex, a viral assembly factor, and ..
  28. ncbi A C-terminal basic amino acid motif of Zaire ebolavirus VP35 is essential for type I interferon antagonism and displays high identity with the RNA-binding domain of another interferon antagonist, the NS1 protein of influenza A virus
    Amy L Hartman
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road MS G 14 Atlanta, GA 30329, USA
    Virology 328:177-84. 2004
    The ebolavirus VP35 protein antagonizes the cellular type I interferon response by blocking phosphorylation of IRF-3, a transcription factor that turns on the expression of a large number of antiviral genes...
  29. ncbi The VP35 and VP40 proteins of filoviruses. Homology between Marburg and Ebola viruses
    A A Bukreyev
    Institute of Molecular Biology, Koltsovo, Novosibirsk Region, Russian Federation
    FEBS Lett 322:41-6. 1993
    ..These fragments were found to encode the VP35 and VP40 proteins. The canonic sequences were revealed before and after each open reading frame...
  30. ncbi GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases
    V E Volchkov
    State Research Centre of Virology and Biotechnology Vector, Institute of Molecular Biology, Koltsovo, Novosibirsk Region, Russia
    Virology 214:421-30. 1995
    ..Our data indicate that the editing site in the Ebola virus glycoprotein gene is recognized not only by Ebola virus polymerase but also by DNA-dependent RNA polymerases of different origin...
  31. pmc Processing of the Ebola virus glycoprotein by the proprotein convertase furin
    V E Volchkov
    Institut fur Virologie, Philipps Universitat Marburg, 35011 Marburg, Germany
    Proc Natl Acad Sci U S A 95:5762-7. 1998
    ..As a result of these observations, it should now be considered that proteolytic processing of GP may be an important determinant for the pathogenicity of Ebola virus...
  32. ncbi Release of viral glycoproteins during Ebola virus infection
    V E Volchkov
    Institut fur Virologie, Philipps Universitat Marburg, Germany
    Virology 245:110-9. 1998
    ..The abundant shedding of soluble GP1 may play an important role in the immunopathology of Ebola hemorrhagic fever in experimentally and naturally infected hosts...
  33. pmc Comparison of the transcription and replication strategies of marburg virus and Ebola virus by using artificial replication systems
    E Muhlberger
    Institut für Virologie der Philipps Universität Marburg, 35037 Marburg, Germany
    J Virol 73:2333-42. 1999
    ..As it was shown previously for MBGV, three of the four EBOV nucleocapsid proteins, NP, VP35, and L, were essential and sufficient for replication...
  34. ncbi Characterization of the L gene and 5' trailer region of Ebola virus
    V E Volchkov
    Institut fur Virologie, Philipps Universitat, Marburg, Germany
    J Gen Virol 80:355-62. 1999
    ..The presence of several conserved linear domains commonly found within L proteins of other members of the order Mononegavirales identified this protein as the RNA-dependent RNA polymerase of Ebola virus...
  35. ncbi The pathogenesis of Ebola hemorrhagic fever
    A Takada
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Trends Microbiol 9:506-11. 2001
    ....
  36. ncbi Biosynthesis and role of filoviral glycoproteins
    H Feldmann
    Canadian Science Centre for Human and Animal Health, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2
    J Gen Virol 82:2839-48. 2001
  37. pmc Ebola virus transcription activator VP30 is a zinc-binding protein
    Jens Modrof
    Institut für Virologie der Philipps Universität Marburg, 35037 Marburg, Germany
    J Virol 77:3334-8. 2003
    ....
  38. pmc The Ebola virus VP35 protein is a suppressor of RNA silencing
    Joost Haasnoot
    Laboratory of Experimental Virology, Department of Medical Microbiology, Center of Infection and Immunity Amsterdam, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands
    PLoS Pathog 3:e86. 2007
    ..Here, we demonstrate that the Ebola virus VP35 protein is a suppressor of RNAi in mammalian cells and that its RSS activity is functionally equivalent to that of ..
  39. ncbi Rescue of Ebola virus from cDNA using heterologous support proteins
    Steven Theriault
    Special Pathogens Program, National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Health Canada, Canada
    Virus Res 106:43-50. 2004
    ..Protein interactions between the nucleoprotein NP and the virion protein VP35 and the polymerase L and VP35 seemed to be the most critical steps...
  40. pmc RNA polymerase I-driven minigenome system for Ebola viruses
    Allison Groseth
    National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada
    J Virol 79:4425-33. 2005
    ....
  41. pmc Homo-oligomerization facilitates the interferon-antagonist activity of the ebolavirus VP35 protein
    St Patrick Reid
    Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Virology 341:179-89. 2005
    We have identified a putative coiled-coil motif within the amino-terminal half of the ebolavirus VP35 protein...
  42. pmc Gene-specific countermeasures against Ebola virus based on antisense phosphorodiamidate morpholino oligomers
    Kelly L Warfield
    US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA
    PLoS Pathog 2:e1. 2006
    ..A combination of EBOV-specific PMOs targeting sequences of viral mRNAs for the viral proteins (VPs) VP24, VP35, and RNA polymerase L protected rodents in both pre- and post-exposure therapeutic regimens...
  43. pmc Global suppression of the host antiviral response by Ebola- and Marburgviruses: increased antagonism of the type I interferon response is associated with enhanced virulence
    John C Kash
    Department of Microbiology, School of Medicine, University of Washington, Box 358070, Seattle, Washington 98195 8070, USA
    J Virol 80:3009-20. 2006
    ..Taken together, these studies showed a correlation between antagonism of type I IFN responses and filovirus virulence...
  44. pmc Functional mapping of the nucleoprotein of Ebola virus
    Shinji Watanabe
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Dr, Madison, Wisconsin 53706, USA
    J Virol 80:3743-51. 2006
    ..2002) previously demonstrated that NP, together with the minor matrix protein VP24 and polymerase cofactor VP35, is necessary and sufficient for the formation of nucleocapsid-like structures that are morphologically ..
  45. pmc Ebola virus VP35-VP40 interaction is sufficient for packaging 3E-5E minigenome RNA into virus-like particles
    Reed F Johnson
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce St, Philadelphia, PA 19104, USA
    J Virol 80:5135-44. 2006
    ..A mammalian two-hybrid assay and confocal microscopy were used to demonstrate that Ebola virus VP35 and VP40 interact and colocalize in transfected cells...
  46. ncbi Descriptive analysis of Ebola virus proteins
    L H Elliott
    Virology 147:169-76. 1985
    ..Seven virion proteins were described; an L (180K), GP (125K), NP (104K), VP40 (40K), VP35 (35K), VP30 (30K), and VP24 (24K)...
  47. ncbi [The properties of Ebola virus proteins]
    E L Subbotina
    Vopr Virusol 51:4-10. 2006
    ..It also presents information on the role of structural (NP, VP40, VP35, GP, VP30, VP24, and L) and secreted (sGP, delta-peptide, GP1, GP(1,2delta), ssGP) proteins in the viral ..

Research Grants2

  1. EBOLA VIRUS INTERFERON-ANTAGONISTS AND VIRULENCE
    CHRISTOPHER BASLER; Fiscal Year: 2003
    ..I previously demonstrated that the Ebola virus VP35 protein functions as an IFN antagonist...
  2. Lassa and Nipah Virus Interferon-Antagonists
    CHRISTOPHER BASLER; Fiscal Year: 2003
    ..abstract_text> ..