VP30

Summary

Gene Symbol: VP30
Description: minor nucleoprotein
Alias: polymerase complex protein
Species: Zaire ebolavirus

Top Publications

  1. ncbi Phosphorylation of VP30 impairs ebola virus transcription
    Jens Modrof
    Institut für Virologie der Philipps Universität Marburg, Robert Koch Strasse 17, Marburg 35037, Germany
    J Biol Chem 277:33099-104. 2002
  2. pmc Role of Ebola virus VP30 in transcription reinitiation
    Miguel J Martínez
    INSERM, U758, Filovirus Laboratory, 21 Av Tony Garnier, 69365 Lyon, Cedex 07, France
    J Virol 82:12569-73. 2008
  3. doi Detection of links between Ebola nucleocapsid and virulence using disorder analysis
    Gerard Kian Meng Goh
    Goh s BioComputing, Singapore, Republic of Singapore
    Mol Biosyst 11:2337-44. 2015
  4. pmc Role of protein phosphatase 1 in dephosphorylation of Ebola virus VP30 protein and its targeting for the inhibition of viral transcription
    Philipp A Ilinykh
    From the Departments of Pathology and Galveston National Laboratory, Galveston, Texas 77555
    J Biol Chem 289:22723-38. 2014
  5. ncbi Oligomerization of Ebola virus VP30 is essential for viral transcription and can be inhibited by a synthetic peptide
    Bettina Hartlieb
    Institut für Virologie der Philipps Universität Marburg, Robert Koch Strasse 17, 35037 Marburg, Germany
    J Biol Chem 278:41830-6. 2003
  6. pmc Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway
    Giulia Fabozzi
    Biodefense Research Section, Vaccine Research Center, National Institutes of Health, Vaccine Research Center, 40 Convent Drive, Bldg 40 2509, Bethesda, MD 20892, USA
    J Virol 85:2512-23. 2011
  7. ncbi How Ebola and Marburg viruses battle the immune system
    Mansour Mohamadzadeh
    US Army Medical Research Institute for Infectious Diseases, Frederick, Maryland, USA
    Nat Rev Immunol 7:556-67. 2007
  8. pmc Ebola virus VP30 is an RNA binding protein
    Sinu P John
    Graduate Program in Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Virol 81:8967-76. 2007
  9. ncbi [The properties of Ebola virus proteins]
    E L Subbotina
    Vopr Virusol 51:4-10. 2006
  10. pmc Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30
    Sven Enterlein
    Department of Virology, Philipps University Marburg, Robert Koch str 17, 35037 Marburg, Germany
    J Virol 80:1038-43. 2006

Scientific Experts

  • Gerard Kian Meng Goh
  • Mansour Mohamadzadeh
  • Heinz Feldmann
  • Jens Modrof
  • V E Volchkov
  • Stephan Becker
  • Elke Mühlberger
  • Hans Dieter Klenk
  • Philipp A Ilinykh
  • Allison Groseth
  • Bettina Hartlieb
  • Steven Theriault
  • Giulia Fabozzi
  • Michael Weik
  • Miguel J Martínez
  • Sinu P John
  • Valentina Volchkova
  • Viktor Volchkov
  • E L Subbotina
  • Sven Enterlein
  • H D Klenk
  • V A Volchkova
  • A A Chepurnov
  • Andrey Ivanov
  • Alexander Bukreyev
  • Alexander N Freiberg
  • Dmytro Kovalskyy
  • Vasiliy S Naumchik
  • Maxim O Platonov
  • Yuri Obukhov
  • Tatiana Ammosova
  • Sergei Nekhai
  • Namita Kumari
  • Tania Garron
  • Bersabeh Tigabu
  • Christopher S Nabel
  • Nancy J Sullivan
  • Michael A Dolan
  • E Muhlberger
  • Olivier Reynard
  • Nathalie Alazard-Dany
  • Nadine Biedenkopf
  • Connie S Schmaljohn
  • Colleen B Jonsson
  • Sonia Longhi
  • Scott Steffen
  • S V Netesov
  • Tan Wang
  • A V Kachko
  • Larissa Kolesnikova
  • Ramon Flick
  • Gülsah Mehmetoglu
  • S Becker
  • Yoshihiro Kawaoka
  • Gabriele Neumann
  • V M Blinov
  • O Dolnik
  • M Weik
  • W Slenczka
  • A A Bukreyev
  • V A Ternovoj
  • A N Kotov
  • L H Elliott
  • M P Kiley
  • J B McCormick

Detail Information

Publications22

  1. ncbi Phosphorylation of VP30 impairs ebola virus transcription
    Jens Modrof
    Institut für Virologie der Philipps Universität Marburg, Robert Koch Strasse 17, Marburg 35037, Germany
    J Biol Chem 277:33099-104. 2002
    Transcription of the highly pathogenic Ebola virus (EBOV) is dependent on VP30, a constituent of the viral nucleocapsid complex...
  2. pmc Role of Ebola virus VP30 in transcription reinitiation
    Miguel J Martínez
    INSERM, U758, Filovirus Laboratory, 21 Av Tony Garnier, 69365 Lyon, Cedex 07, France
    J Virol 82:12569-73. 2008
    b>VP30 is a phosphoprotein essential for the initiation of Ebola virus transcription. In this work, we have studied the effect of mutations in VP30 phosphorylation sites on the ebolavirus replication cycle by using a reverse genetics system...
  3. doi Detection of links between Ebola nucleocapsid and virulence using disorder analysis
    Gerard Kian Meng Goh
    Goh s BioComputing, Singapore, Republic of Singapore
    Mol Biosyst 11:2337-44. 2015
    ..Comparison of the percentage of disorder (PID) in nucleocapsid proteins VP30 and NP reveals high correlation between nucleocapsid PIDs and the case-fatality rates of EBOV...
  4. pmc Role of protein phosphatase 1 in dephosphorylation of Ebola virus VP30 protein and its targeting for the inhibition of viral transcription
    Philipp A Ilinykh
    From the Departments of Pathology and Galveston National Laboratory, Galveston, Texas 77555
    J Biol Chem 289:22723-38. 2014
    ..The EBOV RNA-dependent polymerase complex includes a filovirus-specific VP30, which is critical for the transcriptional but not replication activity of EBOV polymerase; to support ..
  5. ncbi Oligomerization of Ebola virus VP30 is essential for viral transcription and can be inhibited by a synthetic peptide
    Bettina Hartlieb
    Institut für Virologie der Philipps Universität Marburg, Robert Koch Strasse 17, 35037 Marburg, Germany
    J Biol Chem 278:41830-6. 2003
    ..This process is further dependent on VP30, an essential EBOV-specific transcription factor...
  6. pmc Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway
    Giulia Fabozzi
    Biodefense Research Section, Vaccine Research Center, National Institutes of Health, Vaccine Research Center, 40 Convent Drive, Bldg 40 2509, Bethesda, MD 20892, USA
    J Virol 85:2512-23. 2011
    ..In addition to viral protein 35 (VP35), we found that VP30 and VP40 independently act as SRSs. Here, we present the molecular mechanisms of VP30 and VP35...
  7. ncbi How Ebola and Marburg viruses battle the immune system
    Mansour Mohamadzadeh
    US Army Medical Research Institute for Infectious Diseases, Frederick, Maryland, USA
    Nat Rev Immunol 7:556-67. 2007
    ..Filoviruses have provided thereby a new lens through which to examine the immune system itself...
  8. pmc Ebola virus VP30 is an RNA binding protein
    Sinu P John
    Graduate Program in Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Virol 81:8967-76. 2007
    ..proteins that are necessary and sufficient for replication and transcription of the viral RNAs in vitro; NP, VP30, VP35, and L...
  9. ncbi [The properties of Ebola virus proteins]
    E L Subbotina
    Vopr Virusol 51:4-10. 2006
    ..It also presents information on the role of structural (NP, VP40, VP35, GP, VP30, VP24, and L) and secreted (sGP, delta-peptide, GP1, GP(1,2delta), ssGP) proteins in the viral replication cycle ..
  10. pmc Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30
    Sven Enterlein
    Department of Virology, Philipps University Marburg, Robert Koch str 17, 35037 Marburg, Germany
    J Virol 80:1038-43. 2006
    ..The nucleocapsid protein VP30 of MARV and Ebola virus (EBOV) contains a Zn-binding motif which is important for the function of VP30 as a ..
  11. pmc RNA polymerase I-driven minigenome system for Ebola viruses
    Allison Groseth
    National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada
    J Virol 79:4425-33. 2005
    ....
  12. ncbi Rescue of Ebola virus from cDNA using heterologous support proteins
    Steven Theriault
    Special Pathogens Program, National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Health Canada, Canada
    Virus Res 106:43-50. 2004
    ..In contrast to previous data, viral proteins were able to target heterologous filovirus RNA. Together these results indicated that protein-protein interactions are more critical than protein-RNA interactions...
  13. ncbi Descriptive analysis of Ebola virus proteins
    L H Elliott
    Virology 147:169-76. 1985
    ..Seven virion proteins were described; an L (180K), GP (125K), NP (104K), VP40 (40K), VP35 (35K), VP30 (30K), and VP24 (24K)...
  14. pmc Ebola virus transcription activator VP30 is a zinc-binding protein
    Jens Modrof
    Institut für Virologie der Philipps Universität Marburg, 35037 Marburg, Germany
    J Virol 77:3334-8. 2003
    Ebola virus VP30 is an essential activator of viral transcription. In viral particles, VP30 is closely associated with the nucleocapsid complex...
  15. pmc Ebola virus VP30-mediated transcription is regulated by RNA secondary structure formation
    Michael Weik
    Institut für Virologie der Philipps Universität Marburg, 35037 Marburg, Germany
    J Virol 76:8532-9. 2002
    The nucleocapsid protein VP30 of Ebola virus (EBOV), a member of the Filovirus family, is known to act as a transcription activator. By using a reconstituted minigenome system, the role of VP30 during transcription was investigated...
  16. ncbi Biosynthesis and role of filoviral glycoproteins
    H Feldmann
    Canadian Science Centre for Human and Animal Health, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2
    J Gen Virol 82:2839-48. 2001
  17. ncbi Characterization of the L gene and 5' trailer region of Ebola virus
    V E Volchkov
    Institut fur Virologie, Philipps Universitat, Marburg, Germany
    J Gen Virol 80:355-62. 1999
    ..The presence of several conserved linear domains commonly found within L proteins of other members of the order Mononegavirales identified this protein as the RNA-dependent RNA polymerase of Ebola virus...
  18. pmc Comparison of the transcription and replication strategies of marburg virus and Ebola virus by using artificial replication systems
    E Muhlberger
    Institut für Virologie der Philipps Universität Marburg, 35037 Marburg, Germany
    J Virol 73:2333-42. 1999
    ..In contrast to MBGV, EBOV-specific transcription was dependent on the presence of the fourth nucleocapsid protein, VP30. When EBOV VP30 was replaced by MBGV VP30, EBOV-specific transcription was observed but with lower efficiency...
  19. ncbi Release of viral glycoproteins during Ebola virus infection
    V E Volchkov
    Institut fur Virologie, Philipps Universitat Marburg, Germany
    Virology 245:110-9. 1998
    ..The abundant shedding of soluble GP1 may play an important role in the immunopathology of Ebola hemorrhagic fever in experimentally and naturally infected hosts...
  20. pmc Processing of the Ebola virus glycoprotein by the proprotein convertase furin
    V E Volchkov
    Institut fur Virologie, Philipps Universitat Marburg, 35011 Marburg, Germany
    Proc Natl Acad Sci U S A 95:5762-7. 1998
    ..As a result of these observations, it should now be considered that proteolytic processing of GP may be an important determinant for the pathogenicity of Ebola virus...
  21. ncbi GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases
    V E Volchkov
    State Research Centre of Virology and Biotechnology Vector, Institute of Molecular Biology, Koltsovo, Novosibirsk Region, Russia
    Virology 214:421-30. 1995
    ..Our data indicate that the editing site in the Ebola virus glycoprotein gene is recognized not only by Ebola virus polymerase but also by DNA-dependent RNA polymerases of different origin...
  22. ncbi The VP35 and VP40 proteins of filoviruses. Homology between Marburg and Ebola viruses
    A A Bukreyev
    Institute of Molecular Biology, Koltsovo, Novosibirsk Region, Russian Federation
    FEBS Lett 322:41-6. 1993
    ..It is suggested that these sequences are mRNA extremities and at the same time the regulatory elements for mRNA transcription. Homology between the MBG and EBO proteins was discovered...