DCL4

Summary

Gene Symbol: DCL4
Description: dicer-like 4
Alias: ATDCL4, DICER-LIKE 4, F5O24.210, F5O24_210, dicer-like 4
Species: thale cress

Top Publications

  1. ncbi Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana
    Akihiro Hiraguri
    Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, Saiwaicho 3 5 8, Fuchu, Tokyo 183 8509, Japan
    Plant Mol Biol 57:173-88. 2005
  2. pmc DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures
    Quan Sheng Du
    State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    J Virol 81:9142-51. 2007
  3. pmc Specific requirement of DRB4, a dsRNA-binding protein, for the in vitro dsRNA-cleaving activity of Arabidopsis Dicer-like 4
    Akihito Fukudome
    Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3 5 8 Saiwaicho, Fuchu, Tokyo 183 8509, Japan
    RNA 17:750-60. 2011
  4. pmc Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1
    Feng Qu
    School of Biological Sciences, University of Nebraska, 206 Morrison Center, Lincoln, NE 68583 0900, USA
    Proc Natl Acad Sci U S A 105:14732-7. 2008
  5. ncbi The dsRNA-binding protein DRB4 interacts with the Dicer-like protein DCL4 in vivo and functions in the trans-acting siRNA pathway
    Yukihiro Nakazawa
    Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan
    Plant Mol Biol 63:777-85. 2007
  6. pmc Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing
    Todd Blevins
    Institute of Botany, University of Basel, Schönbeinstrasse 6, 4056 Basel, Switzerland
    Nucleic Acids Res 34:6233-46. 2006
  7. pmc An antagonistic function for Arabidopsis DCL2 in development and a new function for DCL4 in generating viral siRNAs
    Nicolas Bouche
    Laboratoire de Biologie Cellulaire, Institut Jean Pierre Bourgin, INRA, Versailles, France
    EMBO J 25:3347-56. 2006
  8. ncbi Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense
    Angelique Deleris
    Institut de Biologie Moleculaire des Plantes, CNRS Unité Propre de Recherche UPR 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Science 313:68-71. 2006
  9. ncbi Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning
    Ian R Henderson
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California 90095, USA
    Nat Genet 38:721-5. 2006
  10. ncbi DICER-LIKE 4 is required for RNA interference and produces the 21-nucleotide small interfering RNA component of the plant cell-to-cell silencing signal
    Patrice Dunoyer
    Institut de Biologie Moléculaire des Plantes du CNRS UPR 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Nat Genet 37:1356-60. 2005

Scientific Experts

  • Liang Wu
  • Akihiro Hiraguri
  • Toshiyuki Fukuhara
  • Akihito Fukudome
  • Hiromitsu Moriyama
  • Yukihiro Nakazawa
  • Allison C Mallory
  • Herve Vaucheret
  • Chantal Brosseau
  • Xiaofang Ma
  • Xiaofeng Fang
  • Peter Moffett
  • Shuxin Zhang
  • James C Carrington
  • Gireesha Mohannath
  • Hideaki Nagano
  • Olivier Voinnet
  • Virginie Gasciolli
  • Fuquan Liu
  • Anna Jakubiec
  • Haina Qin
  • Mathew G Lewsey
  • Heiko Ziebell
  • John P Carr
  • Feng Qu
  • Miya D Howell
  • Guillaume Moissiard
  • Surekha Katiyar-Agarwal
  • Quan Sheng Du
  • Kristin D Kasschau
  • Ian R Henderson
  • Angelique Deleris
  • Nicolas Bouche
  • Todd Blevins
  • Christophe Himber
  • Zhixin Xie
  • Patrice Dunoyer
  • Guoping Wang
  • Louis Valentin Meteignier
  • Marie Claude Nicole
  • Xiuli Lu
  • Zulong Chen
  • Yupeng Shi
  • Ni Hong
  • Yijun Qi
  • Bin Yu
  • R Cody Buchmann
  • Allie K Adams
  • David M Bisaro
  • Youn Hyung Lee
  • Hui Wang
  • Jamie N Jackel
  • Yuhui Liu
  • Veena Patil
  • Saleha Bakht
  • Caroline Dean
  • Seong Wook Yang
  • Nam Hai Chua
  • Mai Egami
  • Akihiro Kanaya
  • Y Adam Yuan
  • Satoru Machida
  • Jianxing Song
  • Fading Chen
  • Xuelu Huan
  • Xiaohong Ye
  • T Jack Morris
  • Zhong hui Zhang
  • Adam Vivian-Smith
  • Scott A Givan
  • Eneida Abreu Parizotto
  • Hailing Jin
  • Rong Xiang Fang
  • Hui Shan Guo
  • Yuan Yuan Fang
  • Elisabeth J Chapman
  • Christopher M Sullivan
  • Noah Fahlgren
  • Qi Xie
  • Cheng Guo Duan
  • Shang Gao
  • Jason S Cumbie
  • Blake C Meyers
  • Jinsong Bao
  • Frederick Meins
  • Padubidri V Shivaprasad
  • Dominique Lauressergues
  • Hyun Sook Park
  • Xiaoyu Zhang
  • Mikhail M Pooggin

Detail Information

Publications30

  1. ncbi Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana
    Akihiro Hiraguri
    Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, Saiwaicho 3 5 8, Fuchu, Tokyo 183 8509, Japan
    Plant Mol Biol 57:173-88. 2005
    ..We show that DRB4 interacts specifically with DCL4, and HYL1 most strongly interacts with DCL1...
  2. pmc DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures
    Quan Sheng Du
    State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    J Virol 81:9142-51. 2007
    ..hierarchical actions of plant Dicer-like (DCL) proteins are required in the biogenesis process of small RNAs, and DCL4 is the primary producer of vsiRNAs...
  3. pmc Specific requirement of DRB4, a dsRNA-binding protein, for the in vitro dsRNA-cleaving activity of Arabidopsis Dicer-like 4
    Akihito Fukudome
    Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3 5 8 Saiwaicho, Fuchu, Tokyo 183 8509, Japan
    RNA 17:750-60. 2011
    Arabidopsis thaliana Dicer-like 4 (DCL4) produces 21-nt small interfering RNAs from both endogenous and exogenous double-stranded RNAs (dsRNAs), and it interacts with DRB4, a dsRNA-binding protein, in vivo and in vitro...
  4. pmc Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1
    Feng Qu
    School of Biological Sciences, University of Nebraska, 206 Morrison Center, Lincoln, NE 68583 0900, USA
    Proc Natl Acad Sci U S A 105:14732-7. 2008
    ..We confirm previous reports implicating both DCL4 and DCL2 in this process and establish a minor role for DCL3...
  5. ncbi The dsRNA-binding protein DRB4 interacts with the Dicer-like protein DCL4 in vivo and functions in the trans-acting siRNA pathway
    Yukihiro Nakazawa
    Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan
    Plant Mol Biol 63:777-85. 2007
    ..We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo...
  6. pmc Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing
    Todd Blevins
    Institute of Botany, University of Basel, Schönbeinstrasse 6, 4056 Basel, Switzerland
    Nucleic Acids Res 34:6233-46. 2006
    ..In contrast, RNA virus infection is mainly affected by DCL4. While the four DCLs are partially redundant for CaLCuV-induced mRNA degradation, DCL4 in conjunction with RDR6 and ..
  7. pmc An antagonistic function for Arabidopsis DCL2 in development and a new function for DCL4 in generating viral siRNAs
    Nicolas Bouche
    Laboratoire de Biologie Cellulaire, Institut Jean Pierre Bourgin, INRA, Versailles, France
    EMBO J 25:3347-56. 2006
    ..the production of miRNAs and siRNAs by DCL1 in certain circumstances and affects development deleteriously in dcl1 dcl4 and dcl1 dcl3 dcl4 mutant plants, whereas dcl1 dcl2 dcl3 dcl4 quadruple mutant plants are viable...
  8. ncbi Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense
    Angelique Deleris
    Institut de Biologie Moleculaire des Plantes, CNRS Unité Propre de Recherche UPR 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Science 313:68-71. 2006
    ..We show here that virus-derived small RNAs produced by Arabidopsis Dicer-like 4 (DCL4) program an effector complex conferring antiviral immunity...
  9. ncbi Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning
    Ian R Henderson
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California 90095, USA
    Nat Genet 38:721-5. 2006
    ..Here, we study the role of the four Arabidopsis thaliana DICER-LIKE genes (DCL1-DCL4) in these processes...
  10. ncbi DICER-LIKE 4 is required for RNA interference and produces the 21-nucleotide small interfering RNA component of the plant cell-to-cell silencing signal
    Patrice Dunoyer
    Institut de Biologie Moléculaire des Plantes du CNRS UPR 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Nat Genet 37:1356-60. 2005
    ..Five alleles defective in synthesis of 21-nt, but not 24-nt, siRNAs carry mutations in Dicer-like 4 (DCL4) that are involved in biogenesis of trans-acting siRNAs...
  11. pmc DICER-LIKE 4 functions in trans-acting small interfering RNA biogenesis and vegetative phase change in Arabidopsis thaliana
    Zhixin Xie
    Center for Gene Research and Biotechnology and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
    Proc Natl Acad Sci U S A 102:12984-9. 2005
    ..A mutant with defects in DCL4 was identified and analyzed for microRNA- and endogenous, small interfering RNA (siRNA)-related functions...
  12. ncbi Partially redundant functions of Arabidopsis DICER-like enzymes and a role for DCL4 in producing trans-acting siRNAs
    Virginie Gasciolli
    Laboratoire de Biologie Cellulaire, Institut Jean Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
    Curr Biol 15:1494-500. 2005
    ..DCL2 produces some virus-derived siRNAs, and DCL3 produces endogenous RDR2-dependent siRNAs, but the role of DCL4 is unknown. We show that DCL4 is the primary processor of endogenous RDR6-dependent trans-acting siRNAs (tasiRNAs)...
  13. pmc Functional and Genetic Analysis Identify a Role for Arabidopsis ARGONAUTE5 in Antiviral RNA Silencing
    Chantal Brosseau
    Centre SEVE, Departement de Biologie, Universite de Sherbrooke, Sherbrooke, Quebec J1K 2R1, Canada
    Plant Cell 27:1742-54. 2015
    ..In agreement with functional assays, we show that Potexvirus infection induces AGO5 expression and that both AGO2 and AGO5 are required for full restriction of PVX infection in systemic tissues of Arabidopsis. ..
  14. doi CMA33/XCT Regulates Small RNA Production through Modulating the Transcription of Dicer-Like Genes in Arabidopsis
    Xiaofeng Fang
    Graduate Program, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
    Mol Plant 8:1227-36. 2015
    ..of sRNAs in Arabidopsis: DCL1-dependent microRNA (miRNA), DCL3-dependent heterochromatic siRNA (hc-siRNA), and DCL4-dependent trans-acting siRNA (ta-siRNA)...
  15. pmc PRL1, an RNA-binding protein, positively regulates the accumulation of miRNAs and siRNAs in Arabidopsis
    Shuxin Zhang
    Center for Plant Science Innovation and School of Biological Sciences, University of Nebraska Lincoln, Lincoln, Nebraska, United States of America
    PLoS Genet 10:e1004841. 2014
    ..Furthermore, PRL1 interacts with the pri-miRNA processor, DCL1, and the dsRNA processors (DCL3 and DCL4). These results suggest that PRL1 may function as a general factor to promote the production of miRNAs and siRNAs...
  16. doi Different roles for RNA silencing and RNA processing components in virus recovery and virus-induced gene silencing in plants
    Xiaofang Ma
    State Key Laboratory of Agricultural Microbiology, Wuhan, Hubei 430070, PR China College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China Université de Sherbrooke, Departement de Biologie, 2500 Boulevard de l Universite, Sherbrooke J1K 2R1, QC, Canada
    J Exp Bot 66:919-32. 2015
    ..Consistent with this, we found that viral recovery induced increased PB formation and that a decapping mutant (DCP2) showed increased VIGS and virus RNA accumulation, indicating an important role for PBs in eliminating viral RNA. ..
  17. pmc A complex containing SNF1-related kinase (SnRK1) and adenosine kinase in Arabidopsis
    Gireesha Mohannath
    Department of Molecular Genetics, Center for Applied Plant Sciences, and Center for RNA Biology, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 9:e87592. 2014
    ..This study establishes the existence of SnRK1-ADK complexes that may play important roles in energy homeostasis and cellular responses to biotic and abiotic stress. ..
  18. pmc Distinct substrate specificities of Arabidopsis DCL3 and DCL4
    Hideaki Nagano
    Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3 5 8 Saiwaicho, Fuchu, Tokyo 183 8509, Japan
    Nucleic Acids Res 42:1845-56. 2014
    In Arabidopsis thaliana, Dicer-like 3 (DCL3) and Dicer-like 4 (DCL4) cleave long, perfect double-stranded RNAs (dsRNAs) into 24 and 21 nucleotides (nt) small interfering RNAs, respectively, which in turn function in RNA-directed DNA ..
  19. pmc ARGONAUTE 1 homeostasis invokes the coordinate action of the microRNA and siRNA pathways
    Allison C Mallory
    Laboratoire de Biologie Cellulaire, Institut Jean Pierre Bourgin, INRA, Route de Saint Cyr, 78026 Versailles, France
    EMBO Rep 10:521-6. 2009
    ..3 (SGS3)- and SILENCING DEFECTIVE 5 (SDE5)-dependent AGO1 silencing, which also requires DICER-LIKE 2 (DCL2) and DCL4. By varying the efficacy of miR168-guided AGO1 mRNA cleavage, we show that siRNA-mediated AGO1 silencing depends on ..
  20. pmc Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection
    Anna Jakubiec
    Laboratory of Plant Molecular Biology, The Rockefeller University, New York, NY 10065, USA
    Plant J 69:14-25. 2012
    RNA silencing is an important antiviral mechanism in diverse eukaryotic organisms. In Arabidopsis DICER-LIKE 4 (DCL4) is the primary antiviral Dicer, required for the production of viral small RNAs from positive-strand RNA viruses...
  21. doi Cotranscriptional role for Arabidopsis DICER-LIKE 4 in transcription termination
    Fuquan Liu
    Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich, UK
    Science 335:1621-3. 2012
    ..We have discovered a role for the Arabidopsis RNA silencing enzyme DICER-LIKE 4 (DCL4) in transcription termination of an endogenous Arabidopsis gene, FCA...
  22. pmc Roles of dicer-like and argonaute proteins in TAS-derived small interfering RNA-triggered DNA methylation
    Liang Wu
    National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    Plant Physiol 160:990-9. 2012
    ..activities of SUPPRESSOR OF GENE SILENCING3 (SGS3), RNA-DEPENDENT RNA POLYMERASE6 (RDR6), and DICER-LIKE4 (DCL4), and loaded into ARGONAUTE1 (AGO1) proteins to posttranscriptionally regulate several target genes by messenger ..
  23. pmc Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction
    Haina Qin
    Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    RNA 16:474-81. 2010
    ..Here we report the first structure of the DUF283 domain from the Arabidopsis thaliana DCL4. The DUF283 domain adopts an alpha-beta-beta-beta-alpha topology and resembles the structural similarity to the ..
  24. doi Effects of DICER-like proteins 2, 3 and 4 on cucumber mosaic virus and tobacco mosaic virus infections in salicylic acid-treated plants
    Mathew G Lewsey
    Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
    J Gen Virol 90:3010-4. 2009
    ..However, the findings do not exclude RNA silencing from making a contribution to SA-mediated resistance in wild-type plants...
  25. doi Effects of dicer-like endoribonucleases 2 and 4 on infection of Arabidopsis thaliana by cucumber mosaic virus and a mutant virus lacking the 2b counter-defence protein gene
    Heiko Ziebell
    Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
    J Gen Virol 90:2288-92. 2009
    ..Cross-protection by Fny-CMVDelta2b against Fny-CMV infection was not abolished in dcl2, dcl3 or dcl4 mutant plants and was strongest in dcl2/4 double mutants, although in these plants and in dcl4 mutants, Fny-..
  26. pmc A novel class of bacteria-induced small RNAs in Arabidopsis
    Surekha Katiyar-Agarwal
    Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California at Riverside, Riverside, California 92521, USA
    Genes Dev 21:3123-34. 2007
    ..which is dicer independent and requires PIWI subfamily proteins, generation of AtlsiRNA-1 requires DCL1, DCL4, and the ARGONAUTE subfamily protein AGO7. It also depends on HYL1, HEN1, HST1, RDR6, and Pol IV...
  27. pmc Transitivity in Arabidopsis can be primed, requires the redundant action of the antiviral Dicer-like 4 and Dicer-like 2, and is compromised by viral-encoded suppressor proteins
    Guillaume Moissiard
    Institut de Biologie Moleculaire des Plantes, Strasbourg Cedex, France
    RNA 13:1268-78. 2007
    ..Viral suppression of primary siRNA accumulation shows that transitivity can be initiated with minute amounts of DCL4-dependent 21-nucleotide (nt)-long siRNAs, whereas DCL3-dependent 24-nt siRNAs appear dispensable for this process...
  28. pmc Genome-wide analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 pathway in Arabidopsis reveals dependency on miRNA- and tasiRNA-directed targeting
    Miya D Howell
    Center for Genome Research and Biocomputing, Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331, USA
    Plant Cell 19:926-42. 2007
    ..many endogenous transcripts, viruses, and transgenes involves the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 (RDR6/DCL4)-dependent short interfering RNA (siRNA) biogenesis pathway...