prostaglandin endoperoxide synthase

Summary

Gene Symbol: prostaglandin endoperoxide synthase
Description: prostaglandin-endoperoxide synthase 1
Alias: COX-1, COX1, PGHS-1, prostaglandin G/H synthase 1, PGH synthase 1, PHS 1, cyclooxygenase-1, prostaglandin H2 synthase 1, prostaglandin endoperoxide synthase
Species: sheep

Top Publications

  1. ncbi The productive conformation of arachidonic acid bound to prostaglandin synthase
    M G Malkowski
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824 1319, USA
    Science 289:1933-7. 2000
  2. doi Glutathione promotes prostaglandin H synthase (cyclooxygenase)-dependent formation of malondialdehyde and 15(S)-8-iso-prostaglandin F2α
    Dimitrios Tsikas
    Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany
    FEBS Lett 586:3723-30. 2012
  3. ncbi Synthesis and use of iodinated nonsteroidal antiinflammatory drug analogs as crystallographic probes of the prostaglandin H2 synthase cyclooxygenase active site
    P J Loll
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084, USA
    Biochemistry 35:7330-40. 1996
  4. pmc Comparison of cyclooxygenase-1 crystal structures: cross-talk between monomers comprising cyclooxygenase-1 homodimers
    Ranjinder S Sidhu
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Biochemistry 49:7069-79. 2010
  5. pmc Characterization of an AM404 analogue, N-(3-hydroxyphenyl)arachidonoylamide, as a substrate and inactivator of prostaglandin endoperoxide synthase
    MELISSA V TURMAN
    A B Hancock, Jr, Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, and Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 48:12233-41. 2009
  6. pmc Development of prostaglandin endoperoxide synthase expression in the ovine fetal central nervous system and pituitary
    Jason A Gersting
    Department of Physiology and Functional Genomics, University of Florida College of Medicine, Gainesville, 32610 0274, USA
    Gene Expr Patterns 9:603-11. 2009
  7. ncbi Molecular oxygen dependent steps in fatty acid oxidation by cyclooxygenase-1
    Arnab Mukherjee
    Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Biochemistry 46:3975-89. 2007
  8. ncbi Structural analysis of NSAID binding by prostaglandin H2 synthase: time-dependent and time-independent inhibitors elicit identical enzyme conformations
    B S Selinsky
    Department of Chemistry, Villanova University, Pennsylvania 19085, USA
    Biochemistry 40:5172-80. 2001
  9. ncbi Mutational and X-ray crystallographic analysis of the interaction of dihomo-gamma -linolenic acid with prostaglandin endoperoxide H synthases
    E D Thuresson
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    J Biol Chem 276:10358-65. 2001
  10. ncbi The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases
    D L DeWitt
    Department of Biochemistry, Michigan State University, East Lansing 48824
    J Biol Chem 265:5192-8. 1990

Scientific Experts

  • Dimitrios Tsikas
  • Ranjinder S Sidhu
  • MELISSA V TURMAN
  • Jason A Gersting
  • P J Loll
  • R M Garavito
  • Arnab Mukherjee
  • M G Malkowski
  • W L Smith
  • B S Selinsky
  • E D Thuresson
  • S L Ginell
  • Chong Yuan
  • Jullia Y Lee
  • William L Smith
  • Charles E Wood
  • Lawrence J Marnett
  • Christine E Schaub
  • D Picot
  • Philip J Kingsley
  • Justine P Roth
  • David W Brinkley
  • Keng Ming Chang
  • K M Lakkides
  • A M Mulichak
  • C T Sharkey
  • K Gupta
  • C J Rieke
  • D L DeWitt
  • O Ekabo
  • J C Otto
  • G J Roth
  • J P Merlie
  • C Yokoyama
  • T Shimokawa
  • T Takai
  • P Needleman
  • R J Kulmacz
  • D Fagan
  • J Mudd
  • M J Andrews
  • T Tanabe
  • R L Armstrong
  • E T Machuga
  • S A Kraemer
  • J Ozols
  • E F Yao
  • E A El-Harith

Detail Information

Publications18

  1. ncbi The productive conformation of arachidonic acid bound to prostaglandin synthase
    M G Malkowski
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824 1319, USA
    Science 289:1933-7. 2000
    ....
  2. doi Glutathione promotes prostaglandin H synthase (cyclooxygenase)-dependent formation of malondialdehyde and 15(S)-8-iso-prostaglandin F2α
    Dimitrios Tsikas
    Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany
    FEBS Lett 586:3723-30. 2012
    ..We propose mechanisms for the PGHS/AA/GSH-dependent formation of MDA, 15(S)-8-iso-prostaglandin F(2α) and other F(2)-isoprostanes. These mechanisms are supported by clinical observations...
  3. ncbi Synthesis and use of iodinated nonsteroidal antiinflammatory drug analogs as crystallographic probes of the prostaglandin H2 synthase cyclooxygenase active site
    P J Loll
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104 6084, USA
    Biochemistry 35:7330-40. 1996
    ..No evidence is seen for significant protein conformational differences between the iodoindomethacin and iodosuprofen of flurbiprofen complex structures...
  4. pmc Comparison of cyclooxygenase-1 crystal structures: cross-talk between monomers comprising cyclooxygenase-1 homodimers
    Ranjinder S Sidhu
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Biochemistry 49:7069-79. 2010
    ..In ovine PGHS-1 crystallized in the absence of an NSAID, there is an alternative route for substrate entry into the COX site different than the well-known route through the membrane binding domain...
  5. pmc Characterization of an AM404 analogue, N-(3-hydroxyphenyl)arachidonoylamide, as a substrate and inactivator of prostaglandin endoperoxide synthase
    MELISSA V TURMAN
    A B Hancock, Jr, Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, and Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 48:12233-41. 2009
    ..is a derivative of arachidonic acid and has been reported to inhibit arachidonate oxygenation by prostaglandin endoperoxide synthase-1 and -2 (PGHS-1 and -2, respectively)...
  6. pmc Development of prostaglandin endoperoxide synthase expression in the ovine fetal central nervous system and pituitary
    Jason A Gersting
    Department of Physiology and Functional Genomics, University of Florida College of Medicine, Gainesville, 32610 0274, USA
    Gene Expr Patterns 9:603-11. 2009
    In this study, we tested the hypothesis that prostaglandin endoperoxide synthase-1 and -2 (PGHS-1 and PGHS-2) are expressed throughout the latter half of gestation in ovine fetal brain and pituitary...
  7. ncbi Molecular oxygen dependent steps in fatty acid oxidation by cyclooxygenase-1
    Arnab Mukherjee
    Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Biochemistry 46:3975-89. 2007
    ....
  8. ncbi Structural analysis of NSAID binding by prostaglandin H2 synthase: time-dependent and time-independent inhibitors elicit identical enzyme conformations
    B S Selinsky
    Department of Chemistry, Villanova University, Pennsylvania 19085, USA
    Biochemistry 40:5172-80. 2001
    ..Instead, they suggest that the different apparent modes of NSAID binding may result from differences in the speed and efficiency with which inhibitors can perturb the hydrogen bonding network around Arg-120 and Tyr-355...
  9. ncbi Mutational and X-ray crystallographic analysis of the interaction of dihomo-gamma -linolenic acid with prostaglandin endoperoxide H synthases
    E D Thuresson
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    J Biol Chem 276:10358-65. 2001
    ..Ser-530 also influences the substrate specificity of PGHS-1; an S530T substitution causes 40- and 750-fold decreases in oxygenation efficiencies for AA and DHLA, respectively...
  10. ncbi The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases
    D L DeWitt
    Department of Biochemistry, Michigan State University, East Lansing 48824
    J Biol Chem 265:5192-8. 1990
    ..The histidine in this latter sequence is the putative axial heme ligand of these peroxidases. We suggest that the histidine (His-309) of sheep PGG/H synthase sequence is the axial heme ligand of this enzyme...
  11. ncbi N-glycosylation of prostaglandin endoperoxide synthases-1 and -2 and their orientations in the endoplasmic reticulum
    J C Otto
    Department of Biochemistry, Michigan State University, East Lansing 48824
    J Biol Chem 268:18234-42. 1993
    ..A similar orientation is predicted for PGH synthase-2...
  12. ncbi The X-ray crystal structure of the membrane protein prostaglandin H2 synthase-1
    D Picot
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637
    Nature 367:243-9. 1994
    ..The conformation of the membrane-binding motif strongly suggests that the enzyme integrates into only one leaflet of the lipid bilayer and is thus a monotopic membrane protein...
  13. ncbi The structural basis of aspirin activity inferred from the crystal structure of inactivated prostaglandin H2 synthase
    P J Loll
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA
    Nat Struct Biol 2:637-43. 1995
    ..We also observe the product salicylic acid binding at a site consistent with its antagonistic effect on aspirin activity...
  14. ncbi Isolation and covalent structure of the aspirin-modified, active-site region of prostaglandin synthetase
    G J Roth
    Biochemistry 22:4672-5. 1983
    ..The acetylated serine residue was located at position 8 in this sequence. The current study marks this polypeptide sequence as a region related to an active site of the enzyme...
  15. ncbi Primary structure of sheep prostaglandin endoperoxide synthase deduced from cDNA sequence
    C Yokoyama
    Laboratory of Cell Biology, National Cardiovascular Center Research Institute, Osaka, Japan
    FEBS Lett 231:347-51. 1988
    The complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA...
  16. pmc Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence
    D L DeWitt
    Department of Biochemistry, Michigan State University, East Lansing 48824
    Proc Natl Acad Sci U S A 85:1412-6. 1988
    ....
  17. ncbi Isolation and characterization of the complementary DNA for sheep seminal vesicle prostaglandin endoperoxide synthase (cyclooxygenase)
    J P Merlie
    Department of Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 263:3550-3. 1988
    ..The putative glycosylation sites, transmembrane domain, and sequence similarities with functionally related enzymes have been incorporated into a model for the topology of cyclooxygenase in the endoplasmic reticulum...
  18. ncbi Tyrosine 385 of prostaglandin endoperoxide synthase is required for cyclooxygenase catalysis
    T Shimokawa
    Department of Biochemistry, Michigan State University, East Lansing 48824
    J Biol Chem 265:20073-6. 1990
    ....