sid2

Summary

Gene Symbol: sid2
Description: NDR kinase Sid2
Species: Schizosaccharomyces pombe 972h-

Top Publications

  1. pmc Roles of putative Rho-GEF Gef2 in division-site positioning and contractile-ring function in fission yeast cytokinesis
    Yanfang Ye
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 23:1181-95. 2012
  2. pmc Initiation of cytokinesis is controlled through multiple modes of regulation of the Sid2p-Mob1p kinase complex
    Ming Chin Hou
    Department of Molecular Genetics and Microbiology and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell Biol 24:3262-76. 2004
  3. ncbi Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB
    Jennifer L Morrell
    Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Curr Biol 14:579-84. 2004
  4. pmc A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors
    Quan Wen Jin
    Department of Molecular Genetics and Microbiology, and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester 01605, USA
    Genetics 172:2101-12. 2006
  5. pmc The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1
    Chun Ti Chen
    Department of Molecular Genetics and Microbiology and Interdisciplinary Graduate Program, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Curr Biol 18:1594-9. 2008
  6. pmc Antagonistic roles of PP2A-Pab1 and Etd1 in the control of cytokinesis in fission yeast
    Aurelia Lahoz
    Centro Andaluz de Biologia del Desarrollo, Consejo Superior de Investigaciones Cientificas Universidad Pablo de Olavide, 41013 Sevilla, Spain
    Genetics 186:1261-70. 2010
  7. ncbi The protein phosphatase 2A B'-regulatory subunit par1p is implicated in regulation of the S. pombe septation initiation network
    X Le Goff
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges, Switzerland
    FEBS Lett 508:136-42. 2001
  8. ncbi Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis
    N Cueille
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research, Epalinges
    J Cell Sci 114:2649-64. 2001
  9. pmc The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11
    Anna Feoktistova
    Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37212, USA
    Mol Biol Cell 23:1636-45. 2012
  10. ncbi Mob1p interacts with the Sid2p kinase and is required for cytokinesis in fission yeast
    M C Hou
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Curr Biol 10:619-22. 2000

Detail Information

Publications41

  1. pmc Roles of putative Rho-GEF Gef2 in division-site positioning and contractile-ring function in fission yeast cytokinesis
    Yanfang Ye
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 23:1181-95. 2012
    ..Collectively, we propose that Gef2 facilitates and stabilizes Mid1 binding to the medial cortex, where the localized Mid1 specifies the division site and induces contractile-ring assembly...
  2. pmc Initiation of cytokinesis is controlled through multiple modes of regulation of the Sid2p-Mob1p kinase complex
    Ming Chin Hou
    Department of Molecular Genetics and Microbiology and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell Biol 24:3262-76. 2004
    ..Sid2p with a mutation of Thr578 to Ala (T578A) can no longer rescue sid2-250 mutant cells, and this results in reduction of Mob1p binding...
  3. ncbi Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB
    Jennifer L Morrell
    Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Curr Biol 14:579-84. 2004
    ..Our results suggest that the Sid4p-Cdc11p complex organizes a signaling hub on the SPB and that this hub coordinates cell and nuclear division...
  4. pmc A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors
    Quan Wen Jin
    Department of Molecular Genetics and Microbiology, and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester 01605, USA
    Genetics 172:2101-12. 2006
    ..In a genetic screen for suppressors of the SIN mutant sid2-250, we isolated a mutation, ace2-35, in the transcription factor Ace2p...
  5. pmc The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1
    Chun Ti Chen
    Department of Molecular Genetics and Microbiology and Interdisciplinary Graduate Program, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Curr Biol 18:1594-9. 2008
    ..Here we show that the most downstream SIN component, the Ndr-family kinase Sid2, maintains Clp1 in the cytoplasm in late mitosis by phosphorylating Clp1 directly and thereby creating binding ..
  6. pmc Antagonistic roles of PP2A-Pab1 and Etd1 in the control of cytokinesis in fission yeast
    Aurelia Lahoz
    Centro Andaluz de Biologia del Desarrollo, Consejo Superior de Investigaciones Cientificas Universidad Pablo de Olavide, 41013 Sevilla, Spain
    Genetics 186:1261-70. 2010
    ..Two-hybrid assays indicate that Pab1 physically interacts with Mob1, Sid1, Sid2, and Cdc11, suggesting that the phosphatase 2A B-subunit is a component of the SIN complex...
  7. ncbi The protein phosphatase 2A B'-regulatory subunit par1p is implicated in regulation of the S. pombe septation initiation network
    X Le Goff
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges, Switzerland
    FEBS Lett 508:136-42. 2001
    ..Loss of par1p function rescues mutants in cdc11, cdc7, and spg1, but no other SIN mutants. Our data suggest that PP2A-par1p acts as a negative regulator of SIN signalling...
  8. ncbi Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis
    N Cueille
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research, Epalinges
    J Cell Sci 114:2649-64. 2001
    ..In contrast to S. cerevisiae Cdc14p, flp1p is also present on the mitotic spindle and contractile ring. The potential roles of flp1p in cell cycle control are discussed...
  9. pmc The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11
    Anna Feoktistova
    Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37212, USA
    Mol Biol Cell 23:1636-45. 2012
    ..In this paper, we show that the last protein kinase in the signaling cascade, Sid2, feeds back to phosphorylate Cdc11 during mitosis...
  10. ncbi Mob1p interacts with the Sid2p kinase and is required for cytokinesis in fission yeast
    M C Hou
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Curr Biol 10:619-22. 2000
    ..for Sid2p localization, and Mob1p localization requires the function of the cdc7, cdc11, cdc14, spg1, sid1, sid2, and sid4 genes, suggesting that together with Sid2p, Mob1p functions at the end of the signaling cascade required ..
  11. ncbi The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation
    E Salimova
    Cell Cycle Control Laboratory, ISREC, Chemin des Boveresses, 1066 Epalinges, Switzerland
    J Cell Sci 113:1695-704. 2000
    ..We also demonstrate that mob1p can be precipitated from cells in a complex with the septation regulating kinase sid2p...
  12. pmc Drc1p/Cps1p, a 1,3-beta-glucan synthase subunit, is essential for division septum assembly in Schizosaccharomyces pombe
    J Liu
    Cell Division Laboratory, Institute of Molecular Agrobiology, The National University of Singapore, Singapore 117604
    Genetics 153:1193-203. 1999
    ..We discuss possible mechanisms that account for the differences in the phenotypes of the drc1-191 and the drc1-null mutants and also reflect the potential links between Drc1p and other cytokinesis regulators...
  13. pmc Isolation and characterization of new fission yeast cytokinesis mutants
    M K Balasubramanian
    Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genetics 149:1265-75. 1998
    ..Four mutants defective in septum deposition, septum initiation defective (sid)1, sid2, sid3, and sid4, were also identified and characterized...
  14. pmc Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis
    C A Sparks
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Cell Biol 146:777-90. 1999
    ....
  15. pmc Characterization of the roles of Blt1p in fission yeast cytokinesis
    John W Goss
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8103Department of Biological Sciences, Wellesley College, Wellesley, MA 02481 8203
    Mol Biol Cell 25:1946-57. 2014
    ..We find three defects that likely contribute to this delay. Without Blt1p, contractile rings recruited and retained less Sid2p/Mob1p and Clp1p phosphatase, and β-glucan synthase Bgs1p accumulated slowly at the cleavage site. ..
  16. pmc CK1 is required for a mitotic checkpoint that delays cytokinesis
    Alyssa E Johnson
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Curr Biol 23:1920-6. 2013
    ..Like Dma1, CK1 accumulates at SPBs during a mitotic arrest and associates stably with SPB components, including Sid4. Our results establish CK1 as an integral component of a mitotic, ubiquitin-mediated checkpoint pathway...
  17. ncbi The 14-3-3 protein rad24p modulates function of the cdc14p family phosphatase clp1p/flp1p in fission yeast
    Mithilesh Mishra
    Cell Division Laboratory, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604
    Curr Biol 15:1376-83. 2005
    ..Furthermore, SIN-dependent nuclear exclusion of Clp1p is dependent on Rad24p function. We conclude that Rad24p-mediated cytoplasmic retention of Clp1p/Flp1p is important for cell viability upon stress to the division apparatus...
  18. pmc SIN-dependent phosphoinhibition of formin multimerization controls fission yeast cytokinesis
    K Adam Bohnert
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genes Dev 27:2164-77. 2013
    ..Here, we identify the essential cytokinetic formin Cdc12 as a key CR substrate of SIN kinase Sid2. Eliminating Sid2-mediated Cdc12 phosphorylation leads to persistent Cdc12 clustering, which prevents CR assembly ..
  19. pmc Measuring affinities of fission yeast spindle pole body proteins in live cells across the cell cycle
    Chad D McCormick
    Departments of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut
    Biophys J 105:1324-35. 2013
    ..These measurements allowed us to account for the fluctuations in Sid2p binding to SPBs throughout the cell cycle. ..
  20. pmc Fission yeast nucleolar protein Dnt1 regulates G2/M transition and cytokinesis by downregulating Wee1 kinase
    Zhi yong Yu
    State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361102, Fujian, China
    J Cell Sci 126:4995-5004. 2013
    ..Our findings also define a novel mode of regulation of Wee1 and Cdk1, which is important for integration of the signals controlling the SIN pathway in fission yeast...
  21. pmc A new genetic method for isolating functionally interacting genes: high plo1(+)-dependent mutants and their suppressors define genes in mitotic and septation pathways in fission yeast
    C F Cullen
    Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Genetics 155:1521-34. 2000
    ..Among the 14 pld mutants with septation defects, 12 mapped to known loci: cdc7, cdc15, cdc11 spg1, and sid2. One of the pld mutants, cdc7-PD1, was selected for suppressor analysis...
  22. pmc Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways
    Sneha Gupta
    Department of Microbiology and Physiological Systems and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Curr Biol 23:333-8. 2013
    ..we developed a proteomics approach that allowed us to identify multiple substrates of the SIN effector kinase Sid2, including the MOR pathway components Nak1 kinase and an associated protein, Sog2...
  23. pmc The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning
    Sebastian Mana-Capelli
    Department of Microbiology and Physiological Systems and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 23:4592-600. 2012
    ..of Klp2 from MTs in anaphase, and we find that this is mediated by direct phosphorylation of Klp2 by the SIN kinase Sid2, which abrogates loading of Klp2 onto MTs by inhibiting its interaction with Mal3 (EB1 homologue)...
  24. pmc The S. pombe cytokinesis NDR kinase Sid2 activates Fin1 NIMA kinase to control mitotic commitment through Pom1/Wee1
    Agnes Grallert
    CRUK Cell Division Group, Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK
    Nat Cell Biol 14:738-45. 2012
    ..We now show that the NDR (nuclear Dbf2-related) kinase component of the fission yeast SIN, Sid2-Mob1, acts independently of the other known SIN components in G2 phase of the cell cycle to control the timing of ..
  25. doi Hsp90 interaction with Cdc2 and Plo1 kinases contributes to actomyosin ring condensation in fission yeast
    Andrea Santino
    Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de Olavide CSIC, Carretera de Utrera Km1, 41013 Sevilla, Spain
    Curr Genet 58:191-203. 2012
    ..Overall, our analyses of hsp90-w1 reveal a possible role for this chaperone in medial ring condensation in association with Cdc2 and Plo1 kinases...
  26. pmc Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe orthologs of the peptidyl proyl isomerases that activate PP2A, reveals a role for Ypa2p in the regulation of cytokinesis
    Anupama Goyal
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    Genetics 190:1235-50. 2012
    ..We also isolated a cold-sensitive allele of ppa2, the major protein phosphatase type 2A catalytic subunit, implicating this enzyme as a regulator of the septation initiation network...
  27. ncbi The S. pombe zfs1 gene is required to prevent septation if mitotic progression is inhibited
    N Beltraminelli
    Cell Cycle Control Laboratory, ISREC, Chemin des Boveresses, 1066 Epalinges, Switzerland
    J Cell Sci 112:3103-14. 1999
    ..The genetic interactions of zfs1 with genes regulating septum formation suggest that it may be a modulator of the signal transduction network controlling the onset of septum formation and exit from mitosis...
  28. pmc Evidence of a new role for the high-osmolarity glycerol mitogen-activated protein kinase pathway in yeast: regulating adaptation to citric acid stress
    Clare L Lawrence
    Centre for Biomolecular Science, School of Biology, University of St Andrews, St Andrews, Fife KY16 9ST, United Kingdom
    Mol Cell Biol 24:3307-23. 2004
    ..Supporting this, Mdh1p and Idh1p, both TCA cycle enzymes, were up-regulated in response to citric acid...
  29. ncbi Bypass of the requirement for cdc16p GAP function in Schizosaccharomyces pombe by mutation of the septation initiation network genes
    N Fournier
    Cell Cycle Control Laboratory, ISREC, Epalinges, Switzerland
    Arch Microbiol 175:62-9. 2001
    ..We also demonstrate that increased expression of the dma1 gene can rescue the lethality of a null allele of cdc16. The implications for the regulation of septum formation in fission yeast are discussed...
  30. pmc Mutations in SID2, a novel gene in Saccharomyces cerevisiae, cause synthetic lethality with sic1 deletion and may cause a defect during S phase
    M D Jacobson
    The Rockefeller University, New York, New York 10021, USA
    Genetics 159:17-33. 2001
    ..In this screen, we identified a novel gene, SID2, which encodes an essential protein that appears to be required for DNA replication or repair...
  31. pmc Localization of fission yeast type II myosin, Myo2, to the cytokinetic actin ring is regulated by phosphorylation of a C-terminal coiled-coil domain and requires a functional septation initiation network
    D P Mulvihill
    Department of Biology, University College London, London WC1E 6BT, United Kingdom
    Mol Biol Cell 12:4044-53. 2001
    ..GFP-Myo2S(1518)E rings persisted at 36 degrees C in cdc7-24 but not in another SIN kinase mutant, sid2-250...
  32. pmc The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe
    Samriddha Ray
    Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Cell Biol 190:793-805. 2010
    ..Together, our results reveal a mutually antagonistic relationship between the SIN and MOR pathways, which is important for completion of cytokinesis and coordination of cytoskeletal remodeling at the mitosis-to-interphase transition...
  33. pmc The Scw1 RNA-binding domain protein regulates septation and cell-wall structure in fission yeast
    Jim Karagiannis
    Department of Biology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    Genetics 162:45-58. 2002
    ..scw1 null mutations also suppress the lethality of mutations (cdc11-136, cdc7-24, cdc14-118, sid1-239, sid2-250, sid3-106, sid4-A1, and mob1-1) at all levels of the sid pathway...
  34. pmc The meiosis-specific Sid2p-related protein Slk1p regulates forespore membrane assembly in fission yeast
    Hongyan Yan
    Cell Division Laboratory, Department of Biological Sciences, National University of Singapore, Singapore
    Mol Biol Cell 19:3676-90. 2008
    ..Interestingly, increased expression of the syntaxin Psy1p rescues the sporulation defect of sid2-250 slk1 Delta...
  35. pmc The actomyosin ring recruits early secretory compartments to the division site in fission yeast
    Aleksandar Vjestica
    Cell Dynamics Group, Temasek Life Sciences Laboratory, 117604 Singapore
    Mol Biol Cell 19:1125-38. 2008
    ..Cdc15p overexpression was sufficient to induce tER polarization in interphase. Thus, fission yeast polarizes its entire secretory machinery to the cell division site by utilizing molecular cues provided by the actomyosin ring...
  36. pmc The nucleolar Net1/Cfi1-related protein Dnt1 antagonizes the septation initiation network in fission yeast
    Quan Wen Jin
    Department of Molecular Genetics and Microbiology, and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 18:2924-34. 2007
    ..Unlike Net1/Cfi1, which regulates the MEN through the Cdc14 phosphatase, Dnt1 can inhibit SIN signaling independently of Clp1, suggesting a novel connection between the nucleolus and the SIN pathway...
  37. ncbi Calcineurin is implicated in the regulation of the septation initiation network in fission yeast
    Yabin Lu
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Genes Cells 7:1009-19. 2002
    ..In fission yeast, calcineurin has been implicated in cytokinesis because calcineurin-deleted cells form multiple septa and cell separation is impeded. However, this mechanism remains unclear...
  38. ncbi Mob2p interacts with the protein kinase Orb6p to promote coordination of cell polarity with cell cycle progression
    Ming Chin Hou
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Cell Sci 116:125-35. 2003
    ..Our results suggest that Mob2p, along with Orb6p, is required for coordinating polarized cell growth during interphase with the onset of mitosis...
  39. pmc Etd1p is a novel protein that links the SIN cascade with cytokinesis
    Rafael R Daga
    Centro Andaluz de Biologia del Desarrollo, CSIC Universidad Pablo de Olavide, Carretera de Utrera Km1, Sevilla, Spain
    EMBO J 24:2436-46. 2005
    ..Etd1p peaks during cytokinesis and is degraded by the ubiquitin-dependent 26S-proteasome pathway at the end of septation, providing a mechanism to couple inactivation of SIN to completion of cytokinesis...
  40. pmc Roles of Pdk1p, a fission yeast protein related to phosphoinositide-dependent protein kinase, in the regulation of mitosis and cytokinesis
    Andrea Bimbo
    Cell Division Laboratory, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore
    Mol Biol Cell 16:3162-75. 2005
    ..We also present evidence that the effects of Pdk1p on cytokinesis are likely mediated via the fission yeast anillin-related protein, Mid1p, and the septation initiation network...
  41. pmc Scw1p antagonizes the septation initiation network to regulate septum formation and cell separation in the fission yeast Schizosaccharomyces pombe
    Quan Wen Jin
    Department of Molecular Genetics and Microbiology and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Eukaryot Cell 2:510-20. 2003
    ..In a screen to search for mutations that can rescue the sid2-250 SIN mutant, we obtained scw1-18...