rqh1

Summary

Gene Symbol: rqh1
Description: RecQ type DNA helicase Rqh1
Alias: hus2, rad12, rec9
Species: Schizosaccharomyces pombe 972h-

Top Publications

  1. ncbi The novel gene mus7(+) is involved in the repair of replication-associated DNA damage in fission yeast
    Mika Yokoyama
    Institute for Virus Research, Kyoto University, Shogoinkawahara cho, Kyoto, Japan
    DNA Repair (Amst) 6:770-80. 2007
  2. doi Sumoylation of RecQ helicase controls the fate of dysfunctional telomeres
    Ofer Rog
    Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Mol Cell 33:559-69. 2009
  3. pmc The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast
    Claudette L Doe
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Nucleic Acids Res 32:1480-91. 2004
  4. ncbi Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks
    Claudette L Doe
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    J Biol Chem 277:32753-9. 2002
  5. pmc Topoisomerase III is required for accurate DNA replication and chromosome segregation in Schizosaccharomyces pombe
    Misook Oh
    School of Biological Sciences, Seoul National University, Seoul 151 742, Republic of Korea
    Nucleic Acids Res 30:4022-31. 2002
  6. pmc The severe slow growth of Deltasrs2 Deltarqh1 in Schizosaccharomyces pombe is suppressed by loss of recombination and checkpoint genes
    Mohamed Maftahi
    Department of Environmental Health Sciences, Columbia University, Mailman School of Public Health and College of Physicians and Surgeons, Kolb Building, Room 114, 722 West 168th Street, New York, NY 10032, USA
    Nucleic Acids Res 30:4781-92. 2002
  7. pmc Rhp51-dependent recombination intermediates that do not generate checkpoint signal are accumulated in Schizosaccharomyces pombe rad60 and smc5/6 mutants after release from replication arrest
    Izumi Miyabe
    Genome Dynamics Group, Research Institute for Microbial Disease, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Mol Cell Biol 26:343-53. 2006
  8. pmc Regulation of Mus81-Eme1 Holliday junction resolvase in response to DNA damage
    Pierre Marie Dehé
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7258, Inserm Unité 1068, Centre de Recherche en Cancerologie de Marseille, Institut Paoli Calmettes, France
    Nat Struct Mol Biol 20:598-603. 2013
  9. ncbi A role for the fission yeast Rqh1 helicase in chromosome segregation
    Thein Z Win
    Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK
    J Cell Sci 118:5777-84. 2005
  10. pmc Role for the fission yeast RecQ helicase in DNA repair in G2
    Louise V Laursen
    Department of Molecular Biology, Aarhus University, DK 8000 Aarhus C, Denmark
    Mol Cell Biol 23:3692-705. 2003

Research Grants

Scientific Experts

  • Felicity Z Watts
  • S Davey
  • J M Murray
  • Shao Win Wang
  • Sarah Lambert
  • Dewight R Williams
  • Toru M Nakamura
  • Paul Russell
  • Katsunori Takahashi
  • Masaru Ueno
  • Masashi Yukawa
  • Eiko Tsuchiya
  • John R Yates
  • Michael N Boddy
  • Violena Pietrobon
  • Susan L Forsburg
  • Ismail Iraqui
  • Shinobu Ukimori
  • Antony M Carr
  • Takashi Morishita
  • John Prudden
  • Matthew C Whitby
  • Tatsuya Kibe
  • Fouzia Ahmad
  • Katerina Zabrady
  • Claudette L Doe
  • Stephane Coulon
  • Stephanie Pebernard
  • James Wohlschlegel
  • Akemi Nakano
  • Thomas Caspari
  • Salah Adam Mahyous Saeyd
  • Tara L Mastro
  • Lin Ding
  • Hiroshi Iwasaki
  • Ulrika Norman-Axelsson
  • Audrey Costes
  • Miriam Schonbrun
  • Julie Bonne Køhler
  • Karine Fréon
  • Tomoko Nanbu
  • Dorota Dziadkowiec
  • Pierre Marie Dehé
  • Yoshitake Matsumoto
  • Hideo Shinagawa
  • Taisuke Hiromoto
  • Junko Kanoh
  • Fekret Osman
  • Lynda M Groocock
  • Naoki Kawabata
  • Ryota Imano
  • Justin C Hope
  • Petra Langerak
  • Jong Sook Ahn
  • Eva Mejía-Ramírez
  • William P Dolan
  • Sophie Rozenzhak
  • J Jefferson P Perry
  • Mohamed Maftahi
  • Ofer Rog
  • Tomoichiro Miyoshi
  • Nicholas Willis
  • Pierre Henri L Gaillard
  • Ling Yin
  • Gareth A Cromie
  • Xiaorong Wang
  • Fuat K Khasanov
  • Alison B Ansbach
  • Laura Roseaulin
  • Assen Roguev
  • Stephen E Kearsey
  • Mika Yokoyama
  • Claire L Dovey
  • Clare H McGowan
  • Fuyuki Ishikawa
  • Takashi Toda
  • Grazia D Raffa
  • Victoria Martin
  • Izumi Miyabe
  • Daniel M Sheedy
  • Charly Chahwan
  • Thein Z Win
  • Hirofumi Morikawa
  • Julie Dixon
  • Greg A Freyer
  • Elspeth Stewart
  • Chunyan Liao
  • Lucie Vondrova
  • Jan J Palecek
  • Aaron Alt

Detail Information

Publications76

  1. ncbi The novel gene mus7(+) is involved in the repair of replication-associated DNA damage in fission yeast
    Mika Yokoyama
    Institute for Virus Research, Kyoto University, Shogoinkawahara cho, Kyoto, Japan
    DNA Repair (Amst) 6:770-80. 2007
    ..From these data, we propose that Mus7 functions in the repair of replication-associated DSBs by promoting RAD51-dependent conversion-type recombination downstream of Rad22 and Mus81...
  2. doi Sumoylation of RecQ helicase controls the fate of dysfunctional telomeres
    Ofer Rog
    Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Mol Cell 33:559-69. 2009
    ..Here we show that the RecQ homolog, Rqh1, is sumoylated...
  3. pmc The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast
    Claudette L Doe
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Nucleic Acids Res 32:1480-91. 2004
    ..to a range of DNA damaging agents, rhp51-dependent hyper-recombination and synthetic sickness when combined with rqh1- that is suppressed by deleting rhp51, rhp55 or rhp57...
  4. ncbi Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks
    Claudette L Doe
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    J Biol Chem 277:32753-9. 2002
    ..In fission yeast DNA junctions at stalled replication forks appear to be processed by either the Rqh1 DNA helicase or Mus81-Eme1 endonuclease...
  5. pmc Topoisomerase III is required for accurate DNA replication and chromosome segregation in Schizosaccharomyces pombe
    Misook Oh
    School of Biological Sciences, Seoul National University, Seoul 151 742, Republic of Korea
    Nucleic Acids Res 30:4022-31. 2002
    ..This lethality is suppressed by concomitant loss of rqh1(+), the RecQ helicase...
  6. pmc The severe slow growth of Deltasrs2 Deltarqh1 in Schizosaccharomyces pombe is suppressed by loss of recombination and checkpoint genes
    Mohamed Maftahi
    Department of Environmental Health Sciences, Columbia University, Mailman School of Public Health and College of Physicians and Surgeons, Kolb Building, Room 114, 722 West 168th Street, New York, NY 10032, USA
    Nucleic Acids Res 30:4781-92. 2002
    Our interest in the Schizosaccharomyces pombe RecQ helicase, rqh1+, led us to investigate the function of a related putative DNA helicase, srs2+. We identified the srs2+ homolog in S...
  7. pmc Rhp51-dependent recombination intermediates that do not generate checkpoint signal are accumulated in Schizosaccharomyces pombe rad60 and smc5/6 mutants after release from replication arrest
    Izumi Miyabe
    Genome Dynamics Group, Research Institute for Microbial Disease, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Mol Cell Biol 26:343-53. 2006
    ..These results suggest that Rad60 is required for recombination repair at a step downstream of Rhp51. We propose that Rhp51-dependent DNA structures that cannot activate the mitotic checkpoints accumulate in rad60-1 cells...
  8. pmc Regulation of Mus81-Eme1 Holliday junction resolvase in response to DNA damage
    Pierre Marie Dehé
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7258, Inserm Unité 1068, Centre de Recherche en Cancerologie de Marseille, Institut Paoli Calmettes, France
    Nat Struct Mol Biol 20:598-603. 2013
    ..Mus81-Eme1 activation prevents gross chromosomal rearrangements in cells lacking the BLM-related DNA helicase Rqh1. We propose that linking Mus81-Eme1 DNA damage-induced activation to cell-cycle progression ensures efficient ..
  9. ncbi A role for the fission yeast Rqh1 helicase in chromosome segregation
    Thein Z Win
    Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK
    J Cell Sci 118:5777-84. 2005
    Schizosaccharomyces pombe Rqh1 protein is a member of the RecQ DNA helicase family. Members of this protein family are mutated in several human genome instability syndromes, including Bloom, Werner and Rothmund-Thomson syndromes...
  10. pmc Role for the fission yeast RecQ helicase in DNA repair in G2
    Louise V Laursen
    Department of Molecular Biology, Aarhus University, DK 8000 Aarhus C, Denmark
    Mol Cell Biol 23:3692-705. 2003
    ..We show that Rqh1, the single Schizosaccharomyces pombe homologue, is a 3'-to-5' helicase and exists with Top3 in a high-molecular-..
  11. pmc Slx1-Slx4 are subunits of a structure-specific endonuclease that maintains ribosomal DNA in fission yeast
    Stephane Coulon
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 15:71-80. 2004
    ..Deletion of Slx1 or Rqh1 RecQ-like DNA helicase provokes rDNA contraction, whereas simultaneous elimination of Slx1-Slx4 endonuclease and ..
  12. pmc Cdc2-cyclin B kinase activity links Crb2 and Rqh1-topoisomerase III
    Thomas Caspari
    Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, UK
    Genes Dev 16:1195-208. 2002
    ..Our data suggest a model in which CDK activity links processing of recombination intermediates to cell cycle progression via checkpoint proteins...
  13. pmc Histone H2A phosphorylation controls Crb2 recruitment at DNA breaks, maintains checkpoint arrest, and influences DNA repair in fission yeast
    Toru M Nakamura
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Cell Biol 24:6215-30. 2004
    ..suppressed the IR hypersensitivity of crb2Delta cells by a mechanism that required the RecQ-like DNA helicase Rqh1. We propose that gamma-H2A modulates checkpoint and DNA repair through large-scale recruitment of Crb2 to damaged ..
  14. pmc The F-Box DNA helicase Fbh1 prevents Rhp51-dependent recombination without mediator proteins
    Fekret Osman
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Mol Cell Biol 25:8084-96. 2005
    ..Furthermore, analysis of different fbh1 mutant alleles suggests that both the F-box and helicase activities of Fbh1 contribute to controlling Rhp51...
  15. pmc Recovery of arrested replication forks by homologous recombination is error-prone
    Ismail Iraqui
    Institut Curie, Centre de Recherche, Orsay, France
    PLoS Genet 8:e1002976. 2012
    ..We propose that deletions/insertions, mediated by micro-homology, leading to copy number variations during replication stress may arise by progression of error-prone replication forks restarted by homologous recombination...
  16. pmc Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates
    Takashi Morishita
    Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Mol Cell Biol 25:8074-83. 2005
    ..fbh1 is essential for viability in stationary-phase cells and in the absence of either Srs2 or Rqh1 DNA helicase. In each case, lethality is suppressed by deletion of the recombination gene rhp57...
  17. pmc A postsynaptic role for Rhp55/57 that is responsible for cell death in Deltarqh1 mutants following replication arrest in Schizosaccharomyces pombe
    Justin C Hope
    Graduate Program in Anatomy and Cell Biology, Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA
    Genetics 170:519-31. 2005
    ..In fission yeast, the RecQ helicase, Rqh1, plays a critical role in this process...
  18. pmc Replication fork blockage by RTS1 at an ectopic site promotes recombination in fission yeast
    Jong Sook Ahn
    Department of Biochemistry, University of Oxford, Oxford, UK
    EMBO J 24:2011-23. 2005
    ..In the absence of the RecQ family DNA helicase Rqh1, deletion events increase dramatically, which correlates with the detection of one-sided DNA double-strand breaks ..
  19. pmc Brc1-mediated DNA repair and damage tolerance
    Daniel M Sheedy
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA
    Genetics 171:457-68. 2005
    ..Activation of this pathway through overproduction of Brc1 bypasses a repair defect in smc6-74, reestablishing resolution of lesions by recombination...
  20. pmc Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints
    D T Liang
    Department of Biology, University of California, San Diego, 92093, USA
    Genetics 159:471-86. 2001
    ..Mcm7p is a nuclear protein throughout the cell cycle, and its localization is dependent on the other MCM proteins. Our data suggest that the Mcm3p-Mcm5p dimer interacts with the Mcm4p-Mcm6p-Mcm7p core complex through Mcm7p...
  21. pmc Release of Ku and MRN from DNA ends by Mre11 nuclease activity and Ctp1 is required for homologous recombination repair of double-strand breaks
    Petra Langerak
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS Genet 7:e1002271. 2011
    ..Exo1 is largely responsible for extended resection up to 3.1 kb from a DSB, with an activity dependent on Rqh1 (Sgs1) DNA helicase having a minor role...
  22. doi Homologous recombination restarts blocked replication forks at the expense of genome rearrangements by template exchange
    Sarah Lambert
    Institut Curie Centre National de la Recherche Scientifique, UMR3348, Réponse Cellulaire aux Perturbations de la Réplication, Universite Paris Sud XI, Orsay, France
    Mol Cell 39:346-59. 2010
    ..This template exchange occurs by both Rad51-dependent and -independent mechanisms. We demonstrate that Rqh1, the BLM homolog, limits Rad51-dependent template exchange without affecting fork restart...
  23. pmc Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage
    William P Dolan
    Molecular and Computational Biology Program, University of Southern California, Los Angeles, CA 90089, USA
    Genetics 185:39-53. 2010
    ..We conclude that Hsk1-Dfp1 kinase functions post-initiation to maintain replication fork stability, an activity potentially mediated by the C terminus of Dfp1...
  24. pmc SUMO-targeted ubiquitin ligases in genome stability
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:4089-101. 2007
    ..The DNA repair factor Rad60 and its human homolog NIP45, which contain SLDs, are candidate STUbL targets. Consistently, Rad60 and Slx8-Rfp mutants have similar DNA repair defects...
  25. pmc Topoisomerase III is essential for accurate nuclear division in Schizosaccharomyces pombe
    A Goodwin
    Imperial Cancer Research Fund Laboratories, London, UK
    Nucleic Acids Res 27:4050-8. 1999
    ..cerevisiae ) between topoisomerase III and DNA helicases of the RecQ family, deletion of the rqh1 (+)gene encoding the only known RecQ helicase in S.pombe suppresses lethality in top3 mutants...
  26. ncbi The role of Schizosaccharomyces pombe SUMO ligases in genome stability
    F Z Watts
    Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, U K
    Biochem Soc Trans 35:1379-84. 2007
    ..We report here on a comparison between the properties of the two proteins and discuss potential roles for the proteins...
  27. pmc Fission yeast rad12+ regulates cell cycle checkpoint control and is homologous to the Bloom's syndrome disease gene
    S Davey
    Department of Oncology, Queen s University, Kingston, Ontario, Canada
    Mol Cell Biol 18:2721-8. 1998
    ..Here we describe the cloning of rad12+, which is the fission yeast homolog of BLM and is identical to the recently reported rhq1+ gene...
  28. pmc Role of Schizosaccharomyces pombe RecQ homolog, recombination, and checkpoint genes in UV damage tolerance
    J M Murray
    School of Biological Sciences, Sussex University, Falmer, Brighton, United Kingdom
    Mol Cell Biol 17:6868-75. 1997
    ..We have identified the gene mutated in the rad12.502 strain as a Schizosaccharomyces pombe recQ homolog...
  29. pmc rqh1+, a fission yeast gene related to the Bloom's and Werner's syndrome genes, is required for reversible S phase arrest
    E Stewart
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 16:2682-92. 1997
    ..rqh1+, previously known as hus2+, encodes a putative DNA helicase related to the Escherichia coli RecQ helicase, with particular homology to the ..
  30. pmc RFCCtf18 and the Swi1-Swi3 complex function in separate and redundant pathways required for the stabilization of replication forks to facilitate sister chromatid cohesion in Schizosaccharomyces pombe
    Alison B Ansbach
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA
    Mol Biol Cell 19:595-607. 2008
    ..We propose that RFC(Ctf18) and the Swi1-Swi3 complex function in separate and redundant pathways essential for replication fork stabilization to facilitate sister chromatid cohesion in fission yeast...
  31. pmc Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint
    Nicholas Willis
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 20:819-33. 2009
    ..required for checkpoint-dependant slowing, including the structure-specific endonuclease Mus81 and the helicase Rqh1, which are implicated in replication fork stability and the negative regulation of recombination...
  32. pmc The fission yeast BLM homolog Rqh1 promotes meiotic recombination
    Gareth A Cromie
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    Genetics 179:1157-67. 2008
    ..In this study we show that mutants lacking the Rqh1 protein of the fission yeast Schizosaccharomyces pombe, a RecQ and BLM homolog, have substantially reduced meiotic ..
  33. pmc The top3(+) gene is essential in Schizosaccharomyces pombe and the lethality associated with its loss is caused by Rad12 helicase activity
    M Maftahi
    Division of Environmental Sciences, Joseph Mailman School of Public Health, New York, NY 10032, USA
    Nucleic Acids Res 27:4715-24. 1999
    ..A deletion mutant, rad12-hd ( rad12 is a homolog of Saccharomyces cerevisiae SGS1), partially suppressed the lethality of top3 mutants...
  34. pmc Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA
    Katerina Zabrady
    Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Kamenice 5, Brno, 62500, Czech Republic Laboratory of Functional Genomics and Proteomics, National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kotlarska 2, Brno, 61137, Czech Republic
    Nucleic Acids Res 44:1064-79. 2016
    ..Based on our results, we propose a model for loading of the SMC5/6 complex onto the chromatin. ..
  35. ncbi A double mutant between fission yeast telomerase and RecQ helicase is sensitive to thiabendazole, an anti-microtubule drug
    Shinobu Ukimori
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi Hiroshima, Japan
    Biosci Biotechnol Biochem 76:264-9. 2012
    ..The double mutant between pot1 and RecQ helicase rqh1 is synthetically lethal. We found that the trt1 rqh1 double mutant was not synthetically lethal...
  36. pmc The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1
    Violena Pietrobon
    Institut Curie, Centre de Recherche, Orsay, France Centre National de la Recherche Scientifique, UMR3348, Centre Universitaire, Orsay, France
    PLoS Biol 12:e1001968. 2014
    ..We reveal functional and physical interplays between CAF-1 and the RecQ-helicase Rqh1, the BLM homologue, mutations in which cause Bloom's syndrome, a human disease associating genome instability with ..
  37. pmc Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF)
    Tara L Mastro
    Program in Molecular and Computational BiologyUniversity of Southern California, Los Angeles, California 90089
    Genetics 198:1457-72. 2014
    ..Thus, the XPF/XPA module of the nucleotide excision repair pathway is incorporated into multiple aspects of genome maintenance even in the absence of external DNA damage...
  38. pmc Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response
    Lin Ding
    Program in Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089 2910
    G3 (Bethesda) 4:1373-84. 2014
    ....
  39. pmc Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state
    Salah Adam Mahyous Saeyd
    Genome Biology Group, College of Natural Sciences, School of Biological Sciences, Bangor University, Brambell Building, Deiniol Road, Bangor LL57 2UW, Wales, United Kingdom
    Nucleic Acids Res 42:7734-47. 2014
    ..Taken together, our data support a model such that elevated Cdc2 activity delays the transition of Crb2 from its G1 to its G2 mode by blocking Srs2 DNA helicase and Casein Kinase 1 (Hhp1)...
  40. pmc Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint
    Akemi Nakano
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi Hiroshima, Japan
    Mol Cell Biol 34:1389-97. 2014
    ..In fission yeast, the double mutant between pot1Δ and the helicase-dead point mutant of the RecQ helicase Rqh1 gene (rqh1-hd) accumulates Rad51-dependent recombination intermediates at telomeres and enters mitosis with those ..
  41. pmc Concerted action of the ubiquitin-fusion degradation protein 1 (Ufd1) and Sumo-targeted ubiquitin ligases (STUbLs) in the DNA-damage response
    Julie Bonne Køhler
    Department of Biology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 8:e80442. 2013
    ..Broader functional relationships between Ufd1 and STUbLs conceivably affect numerous cellular processes beyond the DDR...
  42. pmc Fission yeast RecQ helicase Rqh1 is required for the maintenance of circular chromosomes
    Tomoko Nanbu
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi Hiroshima, Japan
    Mol Cell Biol 33:1175-87. 2013
    ..Fission yeast pot1 and RecQ helicase rqh1 double mutants are synthetically lethal, but the mechanism is not fully understood...
  43. pmc Involvement of Schizosaccharomyces pombe rrp1+ and rrp2+ in the Srs2- and Swi5/Sfr1-dependent pathway in response to DNA damage and replication inhibition
    Dorota Dziadkowiec
    Faculty of Biotechnology, Wrocław University, Przybyszewskiego 63 77, 51 148 Wrocław, Poland, Institute of Low Temperature and Structure Research, Polish Academy of Sciences, PO Box 1410, 50 950 Wrocław, Poland and Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, BN1 9RQ, UK
    Nucleic Acids Res 41:8196-209. 2013
    ..defining Rrp1/2, Rad51 (recombinase), Swi5 and Rad57 (HR-mediators) plus the anti-recombinogenic helicases Srs2 and Rqh1. We confirm that Rrp1 and Rrp2 act together with Srs2 and Swi5 and independently of Rad57 and show that Rqh1 also ..
  44. pmc TORC2 is required to maintain genome stability during S phase in fission yeast
    Miriam Schonbrun
    Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel
    J Biol Chem 288:19649-60. 2013
    ..Our findings indicate that TORC2 is required for genome integrity. This may be relevant for the growing amount of evidence implicating TORC2 in cancer development. ..
  45. pmc The RecQ4 orthologue Hrq1 is critical for DNA interstrand cross-link repair and genome stability in fission yeast
    Lynda M Groocock
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, USA
    Mol Cell Biol 32:276-87. 2012
    ..Thus, in hrq1Δ cells, lesions are channeled through the PRR pathway, yielding hyper-recombinant and mutator phenotypes; analogous defects may underlie the genetic instability and diseases associated with RecQ4 dysfunction...
  46. pmc mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation
    Dewight R Williams
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Eukaryot Cell 1:758-73. 2002
    ..It interacts genetically with replication components and causes an S-phase delay when overexpressed. We propose that Mcl1p, like Ctf4p, has a role in regulating DNA replication complexes...
  47. pmc DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast
    Ulrika Norman-Axelsson
    Center for Biosciences, Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden
    PLoS Genet 9:e1003371. 2013
    ..Here we show that Schizosaccharomyces pombe Top3 and its partner Rqh1 are involved in controlling the levels of CENP-A(Cnp1) at centromeres...
  48. pmc Rad3 decorates critical chromosomal domains with gammaH2A to protect genome integrity during S-Phase in fission yeast
    Sophie Rozenzhak
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS Genet 6:e1001032. 2010
    ..Brc1 C-terminal BRCT domain binding to gammaH2A is crucial in the absence of Rqh1(Sgs1), a RecQ DNA helicase required for rDNA maintenance whose human homologs are mutated in patients with Werner, ..
  49. doi Fission yeast Ku protein is required for recovery from DNA replication stress
    Tomoichiro Miyoshi
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Genes Cells 14:1091-103. 2009
    ..In the absence of Rqh1, the fission yeast RecQ helicase, the cells are sensitive to reagents inducing replication stress...
  50. pmc Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation
    Katsunori Takahashi
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1 3 1 Kagamiyama, Higashi Hiroshima 739 8530, Japan
    Mol Cell Biol 31:495-506. 2011
    ..Deletion of Schizosaccharomyces pombe pot1(+) causes immediate telomere loss. S. pombe Rqh1 is a homolog of the human RecQ helicase WRN, which plays essential roles in the maintenance of genomic stability...
  51. pmc Sws1 is a conserved regulator of homologous recombination in eukaryotic cells
    Victoria Martin
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 25:2564-74. 2006
    Rad52-dependent homologous recombination (HR) is regulated by the antirecombinase activities of Srs2 and Rqh1/Sgs1 DNA helicases in fission yeast and budding yeast...
  52. doi Genetic analysis reveals different roles of Schizosaccharomyces pombe sfr1/dds20 in meiotic and mitotic DNA recombination and repair
    Fuat K Khasanov
    Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, Russia
    Curr Genet 54:197-211. 2008
    ..We suggest that Sfr1 acts early in recombination and has a specific role in Rad51 filament assembly, distinct from that of the Rad51 paralogs...
  53. pmc Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival
    Dewight R Williams
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, USA
    Eukaryot Cell 4:166-77. 2005
    ..We take these data to mean that Mcl1p is a dynamic component of the polymerase alpha complex during replication and is important for the replication stress response in fission yeast...
  54. pmc An alternative eukaryotic DNA excision repair pathway
    G A Freyer
    Center for Radiological Research, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Mol Cell Biol 15:4572-7. 1995
    ..Here we report that the UV-sensitive S. pombe rad12-502 mutant lacks SPDE activity...
  55. pmc Characterization of the alternative excision repair pathway of UV-damaged DNA in Schizosaccharomyces pombe
    R Yonemasu
    Institute of Development, Aging and Cancer, Graduate School of Science, Tohoku University, Seiryomachi 4 1, Sendai 980 77, Japan
    Nucleic Acids Res 25:1553-8. 1997
    ..In contrast to a previous report, our genetic analysis showed that rad12 and uvde are not the same gene...
  56. pmc Mutator phenotype induced by aberrant replication
    V F Liu
    Department of Pathology, Stanford University School of Medicine, Stanford, California 94305 5324, USA
    Mol Cell Biol 19:1126-35. 1999
    ....
  57. pmc The role of Schizosaccharomyces pombe Rad32, the Mre11 homologue, and other DNA damage response proteins in non-homologous end joining and telomere length maintenance
    S Wilson
    Biochemistry, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
    Nucleic Acids Res 27:2655-61. 1999
    ..cerevisiae RAD51 and RAD54, respectively) are not affected. However disruption of rad22, rqh1 and rhp9 / crb2 (homologues of the S.cerevisiae RAD52, SGS1 and RAD9 genes) resulted in increased NHEJ activity...
  58. pmc Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity
    H A Snaith
    Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
    Mol Cell Biol 20:7922-32. 2000
    ..These results indicate that the Hsk1p kinase is a potential target of Cds1p regulation and that its activity is required after replication initiation for normal mitosis...
  59. pmc Involvement of Schizosaccharomyces pombe Srs2 in cellular responses to DNA damage
    S W Wang
    Imperial Cancer Research Fund Molecular Oncology Laboratory, University of Oxford Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, UK
    Nucleic Acids Res 29:2963-72. 2001
    ..srs2 caused spontaneous hyper-recombination and UV sensitivity, and simultaneous deletion of the SGS1 homologue rqh1 caused a severe growth defect reminiscent of that seen in the equivalent S.cerevisiae mutant...
  60. ncbi A homologue of the Rad18 postreplication repair gene is required for DNA damage responses throughout the fission yeast cell cycle
    H M Verkade
    Trescowthick Research Laboratories, Peter MacCallum Cancer Institute, Melbourne, Victoria, Australia
    Mol Genet Genomics 265:993-1003. 2001
    ..Genetic analyses show that rhp18 controls a unique DNA damage repair/tolerance pathway that extends beyond the requirement to tolerate damage during S-phase, suggesting a broader definition of the function of this eukaryotic PRR protein...
  61. ncbi Helicase activity is only partially required for Schizosaccharomyces pombe Rqh1p function
    Fouzia Ahmad
    School of Biological Sciences, University of Manchester, Manchester, UK
    Yeast 19:1381-98. 2002
    ..In the fission yeast Schizosaccharomyces pombe, disruption of the rqh1(+) gene, which encodes the single Sz...
  62. ncbi The fission yeast spSet1p is a histone H3-K4 methyltransferase that functions in telomere maintenance and DNA repair in an ATM kinase Rad3-dependent pathway
    Junko Kanoh
    Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto 606 8502, Japan
    J Mol Biol 326:1081-94. 2003
    ..Nevertheless, the pathway induced in spset1 cells cannot substitute for the Mus81/Rqh1 DNA damage tolerance pathway...
  63. pmc Rad62 protein functionally and physically associates with the smc5/smc6 protein complex and is required for chromosome integrity and recombination repair in fission yeast
    Hirofumi Morikawa
    Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Mol Cell Biol 24:9401-13. 2004
    ..rad62-1 is synthetically lethal with mutations in the genes promoting recovery from stalled replication, such as rqh1, srs2, and mus81, and those involved in nucleotide excision repair like rad13 and rad16...
  64. doi Chromosome fusions following telomere loss are mediated by single-strand annealing
    Xiaorong Wang
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Mol Cell 31:463-73. 2008
    ..A comparison with telomere uncapping caused by the absence of the double-stranded telomere-binding protein Taz1 demonstrates that the circumstances and cause of telomere dysfunction profoundly affect which DNA repair pathway is engaged...
  65. ncbi The N-terminal region of the Schizosaccharomyces pombe RecQ helicase, Rqh1p, physically interacts with Topoisomerase III and is required for Rqh1p function
    Fouzia Ahmad
    School of Biological Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester, M13 9PT, UK
    Mol Genet Genomics 273:102-14. 2005
    The Schizosaccharomyces pombe rqh1+ gene encodes a member of the RecQ DNA helicase family. Members of this protein family are essential for the maintenance of genetic integrity...
  66. pmc The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex
    Stephanie Pebernard
    The Scripps Research Institute, Rm MB107, 10550 North Torrey Pines Rd, Molecular Biology, MB 3, La Jolla, CA 92037, USA
    Mol Cell Biol 26:1617-30. 2006
    ..Further, the viability of Nse5/6 mutants depends on Mus81 and Rqh1, factors that resolve or prevent the formation of Holliday junctions...
  67. pmc Meiotic recombination-deficient mutants of Schizosaccharomyces pombe
    A S Ponticelli
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    Genetics 123:45-54. 1989
    ..intergenic) specificity of the corresponding rec+ gene products. None of the mutations specifically inactivated the ade6-M26 hotspot. Additional rec genes may be identified with these methods...
  68. ncbi SUMO-binding motifs mediate the Rad60-dependent response to replicative stress and self-association
    Grazia D Raffa
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 281:27973-81. 2006
    ..In addition, mutation of Rad60 Ser(32) and Ser(34) to alanine is lethal in cells deleted for the RecQ DNA helicase Rqh1. Finally, we find that Rad60 self-associates via its C-terminal SUMO-like domain and putative SUMO-binding motifs.
  69. pmc Fission yeast Cut8 is required for the repair of DNA double-strand breaks, ribosomal DNA maintenance, and cell survival in the absence of Rqh1 helicase
    Stephen E Kearsey
    Department of Zoology, South Parks Road, Oxford OX1 3PS, United Kingdom
    Mol Cell Biol 27:1558-67. 2007
    Schizosaccharomyces pombe Rqh1 is a member of the RecQ DNA helicase family. Members of this protein family are mutated in cancer predisposition diseases, causing Bloom's, Werner, and Rothmund-Thomson syndromes...
  70. pmc Fission yeast Taz1 and RPA are synergistically required to prevent rapid telomere loss
    Tatsuya Kibe
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi Hiroshima 739 8530, Japan
    Mol Biol Cell 18:2378-87. 2007
    ..taz1-d rad11-D223Y double mutants was suppressed by additional mutation of the helicase domain in a RecQ helicase (Rqh1), or by overexpression of Pot1, a single-strand telomere binding protein that is essential for protection of ..
  71. pmc Mms22 preserves genomic integrity during DNA replication in Schizosaccharomyces pombe
    Claire L Dovey
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 90237, USA
    Genetics 177:47-61. 2007
    ..We propose that Mms22 functions directly at the replication fork to maintain genomic integrity in a pathway involving Mus81-Eme1...
  72. ncbi High-throughput genetic interaction mapping in the fission yeast Schizosaccharomyces pombe
    Assen Roguev
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 1700 4th Street San Francisco, California 94158, USA
    Nat Methods 4:861-6. 2007
    ..pombe, but not S. cerevisiae, and will enable a comprehensive analysis of the conservation of genetic interaction networks...
  73. pmc Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation
    Ling Yin
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, FL 33101, USA
    Mol Biol Cell 19:4374-82. 2008
    ....
  74. pmc Partial suppression of the fission yeast rqh1(-) phenotype by expression of a bacterial Holliday junction resolvase
    C L Doe
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    EMBO J 19:2751-62. 2000
    ..The resolvase partially complements the UV and hydroxyurea hypersensitivity and associated aberrant mitoses of an rqh1(-) mutant...
  75. pmc Mus81 is essential for sister chromatid recombination at broken replication forks
    Laura Roseaulin
    Genomes and Genetic, Unite de la Dynamique du Génome, URA 2171 du CNRS, Institut Pasteur, Paris, France
    EMBO J 27:1378-87. 2008
    ..These findings demonstrate that Mus81/Eme1 is the dedicated endonuclease that resolves sister chromatid recombination intermediates during the repair of broken replication forks...
  76. ncbi The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeast Schizosaccharomyces pombe
    V Hegde
    Department of Molecular Biology and Biotechnology, University of Sheffield, U K
    Mol Gen Genet 251:483-92. 1996
    ..There are distinct differences in the epistatic interactions of several of the repair mutations with respect to 5-azaC-mediated DNA damage relative to UV-mediated DNA damage...

Research Grants1

  1. Structure Determination of the Polymerase alpha-primase
    DEWIGHT WILLIAMS; Fiscal Year: 2006
    ..abstract_text> ..