YKU70

Summary

Gene Symbol: YKU70
Description: ATP-dependent DNA helicase YKU70
Alias: HDF1, NES24
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Inactivation of Ku-mediated end joining suppresses mec1Delta lethality by depleting the ribonucleotide reductase inhibitor Sml1 through a pathway controlled by Tel1 kinase and the Mre11 complex
    Yves Corda
    Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
    Mol Cell Biol 25:10652-64. 2005
  2. pmc The Est1 subunit of Saccharomyces cerevisiae telomerase makes multiple contributions to telomere length maintenance
    Sara K Evans
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genetics 162:1101-15. 2002
  3. pmc The MRX complex plays multiple functions in resection of Yku- and Rif2-protected DNA ends
    Diego Bonetti
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS ONE 5:e14142. 2010
  4. pmc Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends
    Anne E Stellwagen
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 17:2384-95. 2003
  5. pmc Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2
    Eleni P Mimitou
    Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA
    EMBO J 29:3358-69. 2010
  6. pmc Telomere capping in non-dividing yeast cells requires Yku and Rap1
    Momchil D Vodenicharov
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
    EMBO J 29:3007-19. 2010
  7. ncbi Cell cycle-dependent regulation of yeast telomerase by Ku
    Timothy S Fisher
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Struct Mol Biol 11:1198-205. 2004
  8. pmc Shelterin-like proteins and Yku inhibit nucleolytic processing of Saccharomyces cerevisiae telomeres
    Diego Bonetti
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS Genet 6:e1000966. 2010
  9. pmc A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization
    Xiaolan Zhao
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:4777-82. 2005
  10. pmc De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks
    Wei Zhang
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Genes Dev 24:502-15. 2010

Research Grants

Scientific Experts

  • Brian Luke
  • M J Ruiz-Gómez
  • T E Wilson
  • Sang Eun Lee
  • Benoit Palancade
  • Kerstin Bystricky
  • Alison A Bertuch
  • Eun Yong Shim
  • Diego Bonetti
  • David Lydall
  • Michela Clerici
  • Marcus E Marvin
  • Brietta L Pike
  • Kihoon Lee
  • Susan M Gasser
  • Xia Jing Tong
  • Nathalie Grandin
  • Giovanna Lucchini
  • Maria Pia Longhese
  • Albert Ribes-Zamora
  • Jörg Heierhorst
  • Daichi Iwasaki
  • Stephanie Smith
  • Xiaolan Zhao
  • Kyungjae Myung
  • Soma Banerjee
  • Linda Rubinstein
  • Sandra Muñoz-Galván
  • Ireneusz Litwin
  • James E Haber
  • Indukuri Srividya
  • Stephanie Kueng
  • Sarah Luke-Glaser
  • Nagesh Pasupala
  • Piotr Dzierzbicki
  • Krishnaveni Mishra
  • Christopher R Lopez
  • Hui Yong Lian
  • Lisa E Hang
  • Xi Chen
  • Steven S Foster
  • Xuefeng Chen
  • Woo Hyun Chung
  • Grzegorz Ira
  • Jia Lin Ma
  • Zhu Zhu
  • Laura Maringele
  • Ana Traven
  • Eleni P Mimitou
  • Wei Zhang
  • Dominika Mániková
  • Momchil D Vodenicharov
  • James M Dewar
  • Jin Qiu Zhou
  • John H J Petrini
  • Yu Zhang
  • Adriana Miele
  • Brian M Wasko
  • Tzung Ju Wu
  • Fei Long Meng
  • Edward J Louis
  • Angela Taddei
  • Catherine L Vandre
  • Jacqueline Franke
  • Matthew Jessulat
  • Erin E Patterson
  • Daniel Durocher
  • Stefanie E Grund
  • Suriyan Ponnusamy
  • Stephen P Jackson
  • Sanchita Krishna
  • Angela T Y Tam
  • Avgi Tsolou
  • Leticia R Vega
  • Maria Valencia-Burton
  • Michael Downey
  • Catherine H Sterling
  • Caren Norden
  • Mobeen Malik
  • Clark C Chen
  • L Driller
  • Virginia A Zakian
  • Jeffrey Linger
  • Jianxin Ye
  • Yves Corda
  • Yoshimasa Yamana
  • Ayako Ui
  • Michel Charbonneau
  • S J Boulton
  • Christine Soustelle

Detail Information

Publications100

  1. pmc Inactivation of Ku-mediated end joining suppresses mec1Delta lethality by depleting the ribonucleotide reductase inhibitor Sml1 through a pathway controlled by Tel1 kinase and the Mre11 complex
    Yves Corda
    Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
    Mol Cell Biol 25:10652-64. 2005
    ..We report that deletion of YKU70 or YKU80 suppresses mec1Delta, but not rad53Delta, lethality...
  2. pmc The Est1 subunit of Saccharomyces cerevisiae telomerase makes multiple contributions to telomere length maintenance
    Sara K Evans
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genetics 162:1101-15. 2002
    ..The isolation of mutations that perturb separate functions of Est1 demonstrates that a telomerase holoenzyme subunit can contribute multiple regulatory roles to telomere length maintenance...
  3. pmc The MRX complex plays multiple functions in resection of Yku- and Rif2-protected DNA ends
    Diego Bonetti
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS ONE 5:e14142. 2010
    ..Thus, resection of DNA ends that are protected by Yku and Rif2 involves multiple functions of the MRX complex that do not necessarily require its nuclease activity...
  4. pmc Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends
    Anne E Stellwagen
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 17:2384-95. 2003
    ..Thus, the interaction between Ku and TLC1 RNA enables telomerase to act at both broken and normal chromosome ends...
  5. pmc Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2
    Eleni P Mimitou
    Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA
    EMBO J 29:3358-69. 2010
    ..radiation sensitivity of a mutant defective for extensive resection (exo1Δ sgs1Δ) cannot be suppressed by the yku70Δ mutation, indicating that Ku suppression is specific to the initiation of resection...
  6. pmc Telomere capping in non-dividing yeast cells requires Yku and Rap1
    Momchil D Vodenicharov
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
    EMBO J 29:3007-19. 2010
    ..However, Yku and Rap1 become crucially important for this function in these cells. After inactivation of Yku70 in G1-arrested cells, moderate but significant telomere degradation occurs...
  7. ncbi Cell cycle-dependent regulation of yeast telomerase by Ku
    Timothy S Fisher
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Struct Mol Biol 11:1198-205. 2004
    ..These data support a model in which Ku recruits telomerase to telomeres in G1 phase when telomerase is inactive and promotes telomerase-mediated telomere lengthening in late S phase...
  8. pmc Shelterin-like proteins and Yku inhibit nucleolytic processing of Saccharomyces cerevisiae telomeres
    Diego Bonetti
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS Genet 6:e1000966. 2010
    ..Thus, chromosome end degradation is controlled by telomeric proteins that specifically inhibit the action of different nucleases...
  9. pmc A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization
    Xiaolan Zhao
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:4777-82. 2005
    ..The substrates for this SUMO ligase include a subunit of the octameric complex, Smc5, and the DNA repair protein Yku70. We further show that the abolition of the SUMO E3 activity of Mms21 leads to such disparate phenotypes as DNA ..
  10. pmc De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks
    Wei Zhang
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Genes Dev 24:502-15. 2010
    ..These studies therefore identify a mechanism by which the ATR family of kinases enforces genome integrity, and a process that underscores the contribution of Cdc13 to the fate of DNA ends...
  11. pmc In Saccharomyces cerevisiae, yKu and subtelomeric core X sequences repress homologous recombination near telomeres as part of the same pathway
    Marcus E Marvin
    Institute of Genetics, University of Nottingham, Medical School, Queens Medical Centre, Nottingham, UK
    Genetics 183:441-51, 1SI-12SI. 2009
    ..Furthermore, core X bestowed this protection as part of the same pathway as yKu. Our findings implicate a role for both yKu and core X in stabilizing the genome against recombination events involving telomeric sequences...
  12. pmc The association of yKu with subtelomeric core X sequences prevents recombination involving telomeric sequences
    Marcus E Marvin
    Institute of Genetics, University of Nottingham, Medical School, Queen s Medical Centre, Nottingham, UK
    Genetics 183:453-67, 1SI-13SI. 2009
    ..We believe our findings support our hypothesis that yKu and core X play a pivotal role in maintaining genome stability through nuclear architecture by mediating a defensive fold-back structure at yeast chromosome ends...
  13. pmc Sequential loading of Saccharomyces cerevisiae Ku and Cdc13p to telomeres
    Tzung Ju Wu
    Institute of Biopharmaceutical Science and Institute of Biochemistry and Molecular Biology, National Yang Ming University, Shih Pai, Taipei, Taiwan, ROC
    J Biol Chem 284:12801-8. 2009
    ..Our results also offer a mechanism that the binding of Cdc13p to telomeres might prevent Yku from initiating DNA double-stranded break repair pathway on telomeres...
  14. pmc Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks
    Eun Yong Shim
    Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
    EMBO J 29:3370-80. 2010
    ..However, Mre11 nuclease activity is essential for resection in the absence of extensive resection enzymes. The results provide new insights into how MRX catalyses end resection and recombination initiation...
  15. pmc Suppression of gross chromosomal rearrangements by yKu70-yKu80 heterodimer through DNA damage checkpoints
    Soma Banerjee
    Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:1816-21. 2006
    ..To understand the mechanism of Ku as a genome gatekeeper, we overexpressed the yKu70-yKu80 heterodimer and monitored the formation of GCRs...
  16. ncbi A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator
    Michael Downey
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada
    Cell 124:1155-68. 2006
    ..Our results therefore indicate that the KEOPS complex promotes both telomere uncapping and telomere elongation...
  17. pmc The functional importance of telomere clustering: global changes in gene expression result from SIR factor dispersion
    Angela Taddei
    Friedrich Miescher Institute for Biomedical Research and National Center for Competence in Research Frontiers in Genetics, CH 4058 Basel, Switzerland
    Genome Res 19:611-25. 2009
    ..This demonstrates that patterns of gene expression can be regulated by changing the spatial distribution of repetitive DNA sequences that bind repressive factors...
  18. pmc Regulation of nuclear positioning and dynamics of the silent mating type loci by the yeast Ku70/Ku80 complex
    Kerstin Bystricky
    Laboratoire de Biologie Moleculaire Eucaryote, Bâtiment IBCG, Universite de Toulouse, Toulouse, France
    Mol Cell Biol 29:835-48. 2009
    ..However, HMLalpha, unlike HMRa and most telomeres, shows increased NE association in a strain lacking yeast Ku70 (yKu70)...
  19. pmc The DNA end-binding protein Ku regulates silencing at the internal HML and HMR loci in Saccharomyces cerevisiae
    Catherine L Vandre
    Department of Cell and Developmental Biology, University of Illinois, Urbana, Illinois 61801, USA
    Genetics 180:1407-18. 2008
    ..Here, we tested whether Ku contributes to silencing at HML or HMR. Mutant analysis revealed that yKu70 and Sir1 act collectively to silence the mating-type genes at HML and HMR...
  20. pmc The Ku complex in silencing the cryptic mating-type loci of Saccharomyces cerevisiae
    Erin E Patterson
    Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706 1532, USA
    Genetics 180:771-83. 2008
    ..Additional ChIP experiments provided evidence that Ku functioned directly at the HM loci. Thus Ku and Sir1 had overlapping roles in silencing the HM loci...
  21. ncbi Mrc1, a non-essential DNA replication protein, is required for telomere end protection following loss of capping by Cdc13, Yku or telomerase
    Nathalie Grandin
    UMR CNRS no 5161, Ecole Normale Superieure de Lyon, IFR128 BioSciences Gerland, 46, Allee d Italie, 69364 Lyon, France
    Mol Genet Genomics 277:685-99. 2007
    ..Here, we report that in the absence of Mrc1, a component of the replication forks, telomeres of cdc13 or yku70 mutants exhibited increased degradation, while telomerase-negative cells displayed accelerated senescence...
  22. pmc The Yku70-Yku80 complex contributes to regulate double-strand break processing and checkpoint activation during the cell cycle
    Michela Clerici
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milan, Italy
    EMBO Rep 9:810-8. 2008
    ..Here, we show that a lack of any of the NHEJ proteins Yku (Yku70-Yku80), Lif1 or DNA ligase IV (Dnl4) increases 5' DSB end degradation in G1 phase, with ykuDelta cells showing the ..
  23. pmc Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining
    Kihoon Lee
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Genetics 176:2003-14. 2007
    ..Sae2 and Tel1 promote MMEJ but inhibit NHEJ, likely by regulating Mre11-dependent ssDNA accumulation at DNA break. Our data support the role of Sae2 and Tel1 in MMEJ and genome integrity...
  24. pmc Sensitivity of yeast strains with long G-tails to levels of telomere-bound telomerase
    Leticia R Vega
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Genet 3:e105. 2007
    ..These data suggest that wild-type levels of telomere-bound telomerase are critical for the viability of strains whose telomeres are already susceptible to degradation...
  25. pmc Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1
    Avgi Tsolou
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
    DNA Repair (Amst) 6:1607-17. 2007
    ..Our observations show that Mrc1, a core component of the replication fork, plays an important role in telomere capping, protecting from nucleases and checkpoint pathways...
  26. pmc Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast
    V Hegde
    Department of Biochemistry and Kaplan Cancer Center, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Nucleic Acids Res 28:2779-83. 2000
    ..However, NHEJ of blunt ends, while very inefficient, is not further reduced by mutations in YKU70, SIR2, SIR3, SIR4 or SRS2, suggesting that this rejoining process occurs by a different mechanism.
  27. pmc Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae
    G T Milne
    Division of Tumor Immunology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Mol Cell Biol 16:4189-98. 1996
    ..Ku8O associates with the product of the HDF1 gene, forming the major DNA end-binding complex of yeast cells...
  28. pmc Saccharomyces cerevisiae Ku70 potentiates illegitimate DNA double-strand break repair and serves as a barrier to error-prone DNA repair pathways
    S J Boulton
    Wellcome CRC Institute, Cambridge, UK
    EMBO J 15:5093-103. 1996
    ..However, yku70 mutations enhance the radiosensitivity of rad52 strains, which are deficient in homologous recombination...
  29. ncbi Silencing factors participate in DNA repair and recombination in Saccharomyces cerevisiae
    Y Tsukamoto
    Department of Molecular Biology, Institute of Medical Science, University of Tokyo, Japan
    Nature 388:900-3. 1997
    ..One of the factors involved in the end-joining process is Hdf1, a yeast homologue of Ku protein...
  30. pmc Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing
    S J Boulton
    Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    EMBO J 17:1819-28. 1998
    ..These data provide important insights into DNA DSB repair and the linkage of this process to telomere length homeostasis and transcriptional silencing...
  31. ncbi The yeast Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activities
    R M Polotnianka
    Department of Biochemistry, Tulane University Medical Center, New Orleans, Louisiana 70112, USA
    Curr Biol 8:831-4. 1998
    ..Furthermore, mutations in HDF1 or HDF2 rapidly reduce telomeric poly (TG1-3) tract size [1-3], hinting also at a possible telomeric function of Ku...
  32. ncbi Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage
    S E Lee
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Cell 94:399-409. 1998
    ..b>hdf1 cells, lacking Ku70p, fail to escape from this RAD9/RAD17-dependent checkpoint...
  33. ncbi Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast
    S G Martin
    Swiss Institute for Experimental Cancer Research, Epalinges
    Cell 97:621-33. 1999
    ..Consistently, yKu- or SIR-deficient strains are hypersensitive to DNA-damaging agents. The release of yKu from telomeric chromatin may allow efficient scanning of the genome for DNA strand breaks...
  34. ncbi A short C-terminal domain of Yku70p is essential for telomere maintenance
    L Driller
    Institute for Biochemistry, University of Munich LMU, Feodor Lynen Str 25, D 81377 Munich, Germany
    J Biol Chem 275:24921-7. 2000
    ..the role of the Yku70p subunit in these two different pathways, we generated C-terminal deletions of the Yku70 protein and examined their ability to complement the phenotypes of a yku70(-) strain...
  35. pmc Est1 protects telomeres and inhibits subtelomeric y'-element recombination
    Xia Jing Tong
    The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
    Mol Cell Biol 31:1263-74. 2011
    ..Our results demonstrate that one major functional role that Est1 brings to the telomerase complex is the capping or protection of telomeres...
  36. pmc EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutants
    Laura Maringele
    School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
    Genes Dev 16:1919-33. 2002
    ..Therefore, subsets of both DNA-damage and spindle checkpoint pathways cooperate to regulate cell division of yku70Delta mutants...
  37. pmc Functional interplay of the Mre11 nuclease and Ku in the response to replication-associated DNA damage
    Steven S Foster
    Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, 1275 York Ave, RRL 901C, New York, NY 10021, USA
    Mol Cell Biol 31:4379-89. 2011
    ..We have previously shown that in Saccharomyces cerevisiae, Ku70 (yKu70) deficiency reduces the ionizing radiation sensitivity of mre11Δ mutants...
  38. ncbi Multiple pathways cooperate in the suppression of genome instability in Saccharomyces cerevisiae
    K Myung
    Ludwig Institute for Cancer Research, University of California San Diego, 92093, USA
    Nature 411:1073-6. 2001
    ..Mutations that inactivate these pathways cause high rates of GCRs and show synergistic interactions, indicating that the pathways that suppress GCRs all compete for the same DNA substrates...
  39. pmc Cell cycle regulation of DNA double-strand break end resection by Cdk1-dependent Dna2 phosphorylation
    Xuefeng Chen
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Nat Struct Mol Biol 18:1015-9. 2011
    ..Poorly recruited dna2T4A S17A S237A and dna2ΔN248 mutant proteins promote resection only in the presence of Exo1, suggesting cross-talk between Dna2- and Exo1-dependent resection pathways...
  40. pmc Ku must load directly onto the chromosome end in order to mediate its telomeric functions
    Christopher R Lopez
    Department of Pediatrics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Genet 7:e1002233. 2011
    ..The extensive telomere defects observed in these mutants lead us to propose that Ku is an integral component of the terminal telomeric cap, where it promotes a specific architecture that is central to telomere function and maintenance...
  41. pmc Telomerase subunit overexpression suppresses telomere-specific checkpoint activation in the yeast yku80 mutant
    S H Teo
    Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK
    EMBO Rep 2:197-202. 2001
    ..In Saccharomyces cerevisiae, deletion of YKU70 or YKU80 results in an inability to grow at 37 degrees C...
  42. pmc Rap1-Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast
    Kunheng Luo
    Department of Biological Chemistry, UCLA School of Medicine, 90095, USA
    Genes Dev 16:1528-39. 2002
    ..Several proteins are involved in telomeric heterochromatin structure including Rap1, Sir2, Sir3, Sir4, yKu70 (Hdf1), yKu80 (Hdf2), and the N termini of histones H3 and H4...
  43. pmc Cdc13 cooperates with the yeast Ku proteins and Stn1 to regulate telomerase recruitment
    N Grandin
    Ecole Normale Superieure, UMR CNRS ENS 5665, Lyon 69364, France
    Mol Cell Biol 20:8397-408. 2000
    ..depended on telomerase (Est2/TLC1) and Est1, a direct regulator of telomerase, but also on the yeast Ku proteins, yKu70/Hdf1 and yKu80/Hdf2, that have been previously implicated in DNA repair and telomere maintenance...
  44. pmc Mre11 and Ku regulation of double-strand break repair by gene conversion and break-induced replication
    Sanchita Krishna
    Department of Molecular Genetics and Microbiology and Cancer Research and Treatment Center, University of New Mexico School of Medicine, Albuquerque, NM 87131, United States
    DNA Repair (Amst) 6:797-808. 2007
    ..BIR is also elevated in rad51 mutants, but yku70Delta did not suppress BIR in a rad51 background. These results indicate that Mre11 functions in Rad51-independent BIR, and that Ku functions in Rad51-dependent BIR...
  45. ncbi Telomere maintenance is dependent on activities required for end repair of double-strand breaks
    C I Nugent
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Biol 8:657-60. 1998
    ..Ku, Cdc13 and telomerase define three epistasis groups required in parallel for telomere maintenance...
  46. ncbi Distinct faces of the Ku heterodimer mediate DNA repair and telomeric functions
    Albert Ribes-Zamora
    Department of Pediatrics, Hematology Oncology Section, Baylor College of Medicine, One Baylor Plaza, BCM225, Houston, Texas 77030 3411, USA
    Nat Struct Mol Biol 14:301-7. 2007
    ..We propose a 'two-face' model for Ku and that divergent evolution of these faces allowed Ku's dual role in NHEJ and telomere maintenance...
  47. pmc The Rad51 pathway of telomerase-independent maintenance of telomeres can amplify TG1-3 sequences in yku and cdc13 mutants of Saccharomyces cerevisiae
    Nathalie Grandin
    Ecole Normale Superieure de Lyon, UMR CNRS 5665, 69364 Lyon, France
    Mol Cell Biol 23:3721-34. 2003
    ..required for type II recombination) in cdc13-1 yku70delta or yku70delta tlc1delta mutants, but also in cdc13-1 YKU70(+) tlc1delta mutants, still permitted type II recombination, but this process was now entirely dependent on the ..
  48. ncbi A domain of Rad9 specifically required for activation of Chk1 in budding yeast
    Richard T Blankley
    School of Biological Sciences, University of Manchester, G38 Stopford Building, Oxford Rd, Manchester, M13 9PT, UK
    J Cell Sci 117:601-8. 2004
    ..This work extends data showing that separable domains within the Rad9 adaptor protein allow it to activate two distinct kinase signalling pathways independently of each other...
  49. pmc Telomere length kinetics assay (TELKA) sorts the telomere length maintenance (tlm) mutants into functional groups
    Linda Rubinstein
    Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel
    Nucleic Acids Res 42:6314-25. 2014
    ..Thus, our telomere kinetics assay uncovers new functional groups, as well as complex genetic interactions between tlm mutants. ..
  50. pmc The MRX Complex Ensures NHEJ Fidelity through Multiple Pathways Including Xrs2-FHA-Dependent Tel1 Activation
    Daichi Iwasaki
    Department of Integrated Protein Functions, Institute for Protein Research, Osaka University, Suita, Osaka, Japan
    PLoS Genet 12:e1005942. 2016
    ..Thus, the Xrs2 FHA domain and Tel1 kinase work in a coordinated manner to maintain DSB repair fidelity. ..
  51. doi Saccharomyces cerevisiae ATM orthologue suppresses break-induced chromosome translocations
    Kihoon Lee
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Nature 454:543-6. 2008
    ....
  52. doi The generation of oxidative stress-induced rearrangements in Saccharomyces cerevisiae mtDNA is dependent on the Nuc1 (EndoG/ExoG) nuclease and is enhanced by inactivation of the MRX complex
    Piotr Dzierzbicki
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    Mutat Res 740:21-33. 2012
    ..This result indicates that the nuclease plays an important role in processing of oxidative stress-induced lesions in the mitochondrial genome...
  53. pmc The Mph1 helicase can promote telomere uncapping and premature senescence in budding yeast
    Sarah Luke-Glaser
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, DKFZ ZMBH Allianz, Heidelberg, Germany
    PLoS ONE 7:e42028. 2012
    ..We thus put forward that Mph1 is a double-edge sword at the telomere, it prevents NHEJ, but promotes senescence in cells with dysfunctional telomeres by increasing the levels of ssDNA...
  54. pmc Yeast transcription termination factor Rtt103 functions in DNA damage response
    Indukuri Srividya
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
    PLoS ONE 7:e31288. 2012
    b>YKu70/YKu80 is a heterodimer that is essential for repair of DNA double strand breaks through non-homologous end joining pathway in the yeast Saccharomyces cerevisiae...
  55. doi Investigations on the role of base excision repair and non-homologous end-joining pathways in sodium selenite-induced toxicity and mutagenicity in Saccharomyces cerevisiae
    Dominika Mániková
    Laboratory of Molecular Genetics, Cancer Research Institute, Vlarska 7, 833 91 Bratislava, Slovak Republic
    Mutagenesis 25:155-62. 2010
    ....
  56. pmc The SUMO E3 ligase Siz2 exerts a locus-dependent effect on gene silencing in Saccharomyces cerevisiae
    Nagesh Pasupala
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
    Eukaryot Cell 11:452-62. 2012
    ..These data support the idea that sumoylation of specific substrates at the nuclear periphery regulates the availability of Sir2 protein at telomeres...
  57. pmc Oxidative stress and replication-independent DNA breakage induced by arsenic in Saccharomyces cerevisiae
    Ireneusz Litwin
    Institute of Experimental Biology, University of Wroclaw, Wroclaw, Poland
    PLoS Genet 9:e1003640. 2013
    ..In G1 phase, arsenic activates DNA damage response only in the absence of the Yku70-Yku80 complex which normally binds to DNA ends and inhibits resection of DSBs...
  58. pmc Competing roles of DNA end resection and non-homologous end joining functions in the repair of replication-born double-strand breaks by sister-chromatid recombination
    Sandra Muñoz-Galván
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Seville, Spain
    Nucleic Acids Res 41:1669-83. 2013
    ..The phenotype of nuclease-deficient MRX mutants is suppressed by ablation of Yku70 or overexpression of Exo1, suggesting a competition between NHEJ and resection factors for DNA ends arising during ..
  59. pmc Regulating repression: roles for the sir4 N-terminus in linker DNA protection and stabilization of epigenetic states
    Stephanie Kueng
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    PLoS Genet 8:e1002727. 2012
    ..N-terminal half of Sir4 is dispensable for SIR-mediated repression of HM loci in vivo, except in strains that lack Yku70 or have weak silencer elements...
  60. pmc Regulation of telomere length by fatty acid elongase 3 in yeast. Involvement of inositol phosphate metabolism and Ku70/80 function
    Suriyan Ponnusamy
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 283:27514-24. 2008
    ..to determine downstream mechanisms, epistasis experiments were performed, and data indicated that ELO3 and YKU70/80 share a common pathway for the regulation of telomere length...
  61. ncbi Regulation of Saccharomyces Rad53 checkpoint kinase during adaptation from DNA damage-induced G2/M arrest
    A Pellicioli
    Istituto F I R C di Oncologia Molecolare and, Dipartimento di Genetica e di Biologia dei, Microrganismi, Universita degli Studi di Milano, 20133, Milan, Italy
    Mol Cell 7:293-300. 2001
    ..We conclude that activation of the checkpoint kinases in response to a single DNA break is cell cycle regulated and that adaptation is an active process by which these kinases are inactivated...
  62. pmc Histone H4 lysine 91 acetylation a core domain modification associated with chromatin assembly
    Jianxin Ye
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Mol Cell 18:123-30. 2005
    ..These results indicate an important role for histone modifications outside the NH2-tail domains in the processes of chromatin assembly, DNA repair, and transcriptional silencing...
  63. ncbi Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae
    Matthew Jessulat
    Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ont, Canada K1S 5B6
    Arch Biochem Biophys 469:157-64. 2008
    ..We propose that one function of the Rtt109-Vps75 interacting protein pair is to affect the efficiency of NHEJ in yeast. Vps75 but not Rtt109 also seem to have an effect on the efficiency of DSB repair using homologous recombination...
  64. pmc A new Saccharomyces cerevisiae strain with a mutant Smt3-deconjugating Ulp1 protein is affected in DNA replication and requires Srs2 and homologous recombination for its viability
    Christine Soustelle
    Commissaritat à l Energy Atomique, UMR 217 CNRS CEA, DSV DRR, Fontenay aux Roses, France
    Mol Cell Biol 24:5130-43. 2004
    ..These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway...
  65. pmc Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncapping
    James M Dewar
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle upon Tyne, Tyne and Wear, UK
    EMBO J 29:4020-34. 2010
    ..Thus, attenuation of the DDR at uncapped telomeres can circumvent the need for otherwise-essential telomere capping proteins...
  66. pmc Yeast Nej1 is a key participant in the initial end binding and final ligation steps of nonhomologous end joining
    Xi Chen
    Radiation Oncology Research Laboratory, Department of Radiation Oncology, and the Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 286:4931-40. 2011
    ....
  67. pmc The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation
    Hui Yong Lian
    Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK
    Mol Biol Cell 22:1753-65. 2011
    ..Genome-wide analysis shows that regions extending up to 80 kb from telomeres replicate abnormally early in a yku70 mutant...
  68. ncbi Telomere instability caused by subtelomeric Y' amplification and rearrangements in Saccharomyces cerevisiae (ku70 tel1 and ku70 rad50) double mutants
    Miguel J Ruiz-Gómez
    Radiobiological Institute, University of Munich Schillerstr 42, 80336 Munich, Germany
    Indian J Exp Biol 49:324-31. 2011
    Telomeres solve the end-replication problem. Previous results suggested a relation between Yku70/80 and proteins Tell and Rad50 in telomere stabilization...
  69. pmc SUMOylation regulates telomere length homeostasis by targeting Cdc13
    Lisa E Hang
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 18:920-6. 2011
    ..cdc13-snm's effect on telomere length is epistatic with stn1, but not with yku70, tel1 or est1 alleles, and is suppressed by Stn1 overexpression...
  70. pmc Multiple pathways promote short-sequence recombination in Saccharomyces cerevisiae
    Glenn M Manthey
    Division of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, 1450 E Duarte Road, Duarte, CA 91010 0269, USA
    Mol Cell Biol 22:5347-56. 2002
    ..Additional evidence suggests that SSR is distinct from nonhomologous end joining and is superimposed upon basal homologous recombination...
  71. pmc Dicentric chromosome stretching during anaphase reveals roles of Sir2/Ku in chromatin compaction in budding yeast
    D A Thrower
    Department of Biology, CB3280 University of North Carolina, Chapel Hill, NC 27599 3280, USA
    Mol Biol Cell 12:2800-12. 2001
    ..In cells deleted for yKU70, yKU80, or SIR2, a 10-kb region of the dicentric chromosome stretched along the spindle axis to a length of 6 ..
  72. ncbi Effects of HDF1 (Ku70) and HDF2 (Ku80) on spontaneous and DNA damage-induced intrachromosomal recombination in Saccharomyces cerevisiae
    T Cervelli
    Genetica e Biochimica Tossicologica, Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy
    Mol Gen Genet 264:56-63. 2000
    ..b>HDF1 and HDF2, which have been identified in Saccharomyces cerevisiae as homologues of the Ku70 and Ku80 proteins of ..
  73. ncbi Efficient processing of DNA ends during yeast nonhomologous end joining. Evidence for a DNA polymerase beta (Pol4)-dependent pathway
    T E Wilson
    Department of Pathology, Division of Laboratory Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 274:23599-609. 1999
    ..Pol4 is thus specifically recruited to perform gap-filling in an NHEJ pathway that must also involve as yet unidentified nucleases...
  74. pmc The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks
    Eun Yong Shim
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mol Cell Biol 25:3934-44. 2005
    ..Recruitment of RSC to a DSB depends on Mre11, Rsc30, and yKu70 proteins. Rsc1p and Rsc2p, two other RSC subunits, physically interact with yKu80p and Mre11p...
  75. pmc The yeast histone chaperone chromatin assembly factor 1 protects against double-strand DNA-damaging agents
    Jeffrey Linger
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center at Fitzsimons, Aurora, CO 80045, USA
    Genetics 171:1513-22. 2005
    ..We propose that CAF-1 has an essential role in assembling chromatin during double-strand-DNA repair...
  76. pmc Yeast silent mating type loci form heterochromatic clusters through silencer protein-dependent long-range interactions
    Adriana Miele
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA, USA
    PLoS Genet 5:e1000478. 2009
    ....
  77. pmc The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
    Stefanie E Grund
    Biochemie Zentrum der Universitat Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 182:897-910. 2008
    ..Our data show that the inner nuclear membrane protein Src1 functions at the interface between subtelomeric gene expression and TREX-dependent messenger RNA export through the nuclear pore complexes...
  78. pmc Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication
    Fei Long Meng
    The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China
    EMBO J 28:1466-78. 2009
    ..Thus, Sua5p represents a novel ssTG DNA-binding protein and positively regulates the telomere length in vivo...
  79. pmc DNA polymerase 4 of Saccharomyces cerevisiae is important for accurate repair of methyl-methanesulfonate-induced DNA damage
    Catherine H Sterling
    Department of Therapeutic Radiology and Genetics, Yale University, New Haven, Connecticut 06510, USA
    Genetics 172:89-98. 2006
    ..The MMS sensitivity of a Pol4-deficient strain can be rescued by deletion of YKu70. We also show that deletion of Pol4 results in a 6- to 14-fold increase in the MMS-induced mutation frequency and ..
  80. ncbi Roles of nonhomologous end-joining pathways in surviving topoisomerase II-mediated DNA damage
    Mobeen Malik
    Department of Molecular Pharmacology, St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, TN 38105, USA
    Mol Cancer Ther 5:1405-14. 2006
    ..Using this approach, we found that yku70 (hdf1), yku80 (hdf2), and other genes required for NHEJ were important for cell survival following exposure to ..
  81. ncbi The NoCut pathway links completion of cytokinesis to spindle midzone function to prevent chromosome breakage
    Caren Norden
    Institute of Biochemistry, Biology Department, ETH Zurich, 8093 Zurich, Switzerland
    Cell 125:85-98. 2006
    ..We propose that NoCut monitors clearance of chromatin from the midzone to ensure that cytokinesis completes only after all chromosomes have migrated to the poles...
  82. pmc Different mating-type-regulated genes affect the DNA repair defects of Saccharomyces RAD51, RAD52 and RAD55 mutants
    Maria Valencia-Burton
    Department of Biology and Resenstiel Center, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Genetics 174:41-55. 2006
    ..All three recombination-defective mutations are made more sensitive by deletions of Rad6 and of the histone deacetylases Rpd3 and Ume6, although these mutations are not themselves CPT or phleomycin sensitive...
  83. pmc Inhibition of DNA double-strand break repair by the Ku heterodimer in mrx mutants of Saccharomyces cerevisiae
    Brian M Wasko
    Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, USA
    DNA Repair (Amst) 8:162-9. 2009
    ..experiments demonstrated that the 5'-end of TLC1 RNA was essential and a segment containing a binding site for the Yku70/Yku80 complex was sufficient for suppression...
  84. doi Dual functions of Mdt1 in genome maintenance and cell integrity pathways in Saccharomyces cerevisiae
    Ana Traven
    St Vincent s Institute of Medical Research and Department of Medicine SVH, University of Melbourne, Fitzroy, Victoria 3065, Australia
    Yeast 27:41-52. 2010
    ..Overall, the results indicate that Mdt1 has partially separable functions in both cell wall and genome integrity pathways...
  85. pmc Mdt1 facilitates efficient repair of blocked DNA double-strand breaks and recombinational maintenance of telomeres
    Brietta L Pike
    St Vincent s Institute of Medical Research, 9 Princes Street, Fitzroy, VIC 3065, Australia
    Mol Cell Biol 27:6532-45. 2007
    ....
  86. pmc Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes
    Benoit Palancade
    Institut Curie, Centre de Recherche, and Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique, F 75248 Paris, France
    Mol Biol Cell 18:2912-23. 2007
    ..for the establishment of the appropriate sumoylation of several cellular proteins, including the DNA repair factor Yku70. Moreover, restoration of nuclear envelope-associated Ulp1 in nucleoporin mutants reestablishes proper sumoylation ..
  87. ncbi Telomere-related functions of yeast KU in the repair of bleomycin-induced DNA damage
    Angela T Y Tam
    St Vincent s Institute of Medical Research, 9 Princes Street, Fitzroy, VIC 3065, Australia
    Biochem Biophys Res Commun 357:800-3. 2007
    ..cerevisiae, DSBs are predominantly repaired by RAD52-dependent homologous recombination (HR) with some support by Yku70/Yku80 (KU)-dependent pathways...
  88. pmc New function of CDC13 in positive telomere length regulation
    B Meier
    Institute for Biochemistry, University of Munich LMU, D 81377 Munich, Germany
    Mol Cell Biol 21:4233-45. 2001
    ..In a synthetic lethality screen with YKU70, the 70-kDa subunit of the telomere-associated Yku heterodimer, we identified a new mutation in CDC13, cdc13-4, ..
  89. doi Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1
    Jacqueline Franke
    Zentrum für Medizinische Biotechnologie, Universitat Duisburg Essen, Universitätsstr 5, 45117 Essen, Germany
    J Cell Sci 121:3553-60. 2008
    ..Furthermore, tgs1Delta cells displayed a shortened replicative lifespan, suggesting that the loss of the m(3)G cap of TLC1 causes premature aging...
  90. pmc NuA4 subunit Yng2 function in intra-S-phase DNA damage response
    John S Choy
    Department of Molecular Genetics and Cell Biology Center for Molecular Oncology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 22:8215-25. 2002
    ..Our results implicate nucleosomal histone acetylation in maintaining genomic integrity during chromosomal replication...
  91. pmc Multiple roles for Saccharomyces cerevisiae histone H2A in telomere position effect, Spt phenotypes and double-strand-break repair
    Holly R Wyatt
    Interdisciplinary Program in Molecular and Cellular Biology, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Genetics 164:47-64. 2003
    ..In the absence of the DSB and telomere-binding protein yKu70, the bleomycin sensitivity of hta1tpe alleles is further enhanced...
  92. ncbi Suppression of homologous recombination by the Saccharomyces cerevisiae linker histone
    Jessica A Downs
    The Wellcome Trust Cancer Research UK Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Mol Cell 11:1685-92. 2003
    ..Finally, we show that Hho1p is inhibitory to the recombination-dependent mechanism of telomere maintenance. The role of linker histones in genome stability, aging, and tumorigenesis is discussed...
  93. pmc Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences
    Jia Lin Ma
    Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Mol Cell Biol 23:8820-8. 2003
    ..The increased gamma ray sensitivity of rad1Delta rad52Delta yku70Delta strains compared to rad52Delta yku70Delta strains suggests that MMEJ also contributes to the repair of DSBs induced by ionizing radiation...
  94. ncbi Regulation of homologous integration in yeast by the DNA repair proteins Ku70 and RecQ
    Yoshimasa Yamana
    Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2 4 Hibikino, Wakamatsu ku, Kitakyushu, 808 0196, Japan
    Mol Genet Genomics 273:167-76. 2005
    ..We present evidence for a genetic interaction between SGS1 and YKU70, which encodes the S. cerevisiae homologue of the human DNA helicase Ku70...
  95. ncbi Genetic analysis of ionizing radiation-induced mutagenesis in Saccharomyces cerevisiae reveals TransLesion Synthesis (TLS) independent of PCNA K164 SUMOylation and ubiquitination
    Clark C Chen
    Department of Neurosurgery, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA
    DNA Repair (Amst) 5:1475-88. 2006
    ..A genetic model based on these observations is proposed...
  96. ncbi Nuclear pore complexes in the organization of silent telomeric chromatin
    V Galy
    Laboratoire de Biologie Cellulaire du Noyau, Institut Pasteur, CNRS URA 1773, Paris, France
    Nature 403:108-12. 2000
    ..The only protein found to be involved in the peripheral localization of telomeres is Yku70/Yku80...
  97. ncbi The ability of Sgs1 to interact with DNA topoisomerase III is essential for damage-induced recombination
    Ayako Ui
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    DNA Repair (Amst) 4:191-201. 2005
    ....
  98. pmc Dynamic regulation of single-stranded telomeres in Saccharomyces cerevisiae
    Stephanie Smith
    Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genetics 178:693-701. 2008
    ..Therefore, the yKu70-80 heterodimer and telomerase maintain telomere size, and the helicase activity of Pif1 likely also helps to ..
  99. ncbi Telomerase, Ku, and telomeric silencing in Saccharomyces cerevisiae
    S K Evans
    Verna and Marrs MacLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, USA
    Chromosoma 107:352-8. 1998
    ....
  100. pmc The Saccharomyces cerevisiae Ku autoantigen homologue affects radiosensitivity only in the absence of homologous recombination
    W Siede
    Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Genetics 142:91-102. 1996
    ..In the yeast Saccharomyces cerevisiae, we have examined the phenotype conferred by a deletion of HDF1, the putative homologue of the 70-kD subunit of the DNA-end binding Ku complex of DNA-PK...

Research Grants1

  1. Mechanisms of Nonhomologous Repair of Damaged DNA
    Sang Eun Lee; Fiscal Year: 2005
    ..Furthermore, since DSB repair by NHEJ is remarkably conserved from yeast to humans, these studies will help to dissect the similar pathways in humans. ..