TOM70

Summary

Gene Symbol: TOM70
Description: protein channel TOM70
Alias: MAS70, MOM72, OMP1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex
    P J Dekker
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
    Mol Cell Biol 18:6515-24. 1998
  2. ncbi Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae
    Lena Burri
    Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Australia
    FEBS J 273:1507-15. 2006
  3. ncbi Crystal structure of yeast mitochondrial outer membrane translocon member Tom70p
    Yunkun Wu
    Department of Cell Biology, Center for Biophysical Sciences and Engineering, MCLM 364, 1918 Univ Blvd, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
    Nat Struct Mol Biol 13:589-93. 2006
  4. ncbi Biogenesis of the mitochondrial TOM complex: Mim1 promotes insertion and assembly of signal-anchored receptors
    Thomas Becker
    Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, D 79104 Freiburg, Germany
    J Biol Chem 283:120-7. 2008
  5. pmc The peripheral membrane subunits of the SAM complex function codependently in mitochondrial outer membrane biogenesis
    Nickie C Chan
    Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria 3010, Australia
    Mol Biol Cell 19:126-36. 2008
  6. ncbi Molecular chaperones Hsp90 and Hsp70 deliver preproteins to the mitochondrial import receptor Tom70
    Jason C Young
    Cellular Biochemistry, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Cell 112:41-50. 2003
  7. pmc Tetratricopeptide repeat proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis
    Noriko Kondo-Okamoto
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112 5650, USA
    EMBO Rep 9:63-9. 2008
  8. pmc Mitochondrial cardiolipin involved in outer-membrane protein biogenesis: implications for Barth syndrome
    Natalia Gebert
    Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
    Curr Biol 19:2133-9. 2009
  9. doi Regulation of mitochondrial protein import by cytosolic kinases
    Oliver Schmidt
    Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, Freiburg, Germany
    Cell 144:227-39. 2011
  10. pmc The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins
    Thomas Becker
    Institute for Biochemistry and Molecular Biology, Centre for Biochemistry and Molecular Cell Research, Freiburg, Germany
    J Cell Biol 194:387-95. 2011

Scientific Experts

  • X Grandier-Vazeille
  • Peter Rehling
  • Koji Okamoto
  • Thomas Becker
  • Chris Meisinger
  • Nikolaus Pfanner
  • Trevor Lithgow
  • Bernard Guiard
  • Agnieszka Chacinska
  • Drazen Papic
  • Nickie C Chan
  • Nils Wiedemann
  • Doron Rapaport
  • Lena Böttinger
  • Susanne E Horvath
  • Sanjana Rao
  • Oliver Schmidt
  • Yunkun Wu
  • Jonathan Melin
  • Terrence D Mulhern
  • Görkem Garipler
  • Lidia Wrobel
  • Maria Bohnert
  • Erez Eliyahu
  • Elisa Merklinger
  • Gunther Daum
  • Lena Sophie Wenz
  • Birgit Schönfisch
  • Angelika B Harbauer
  • Judith M Müller
  • J Brix
  • David A Stroud
  • Dusanka Milenkovic
  • Diana Stojanovski
  • N Pfanner
  • K Dietmeier
  • Natalia Gebert
  • Ryan D Mills
  • Stephan Kutik
  • Noriko Kondo-Okamoto
  • Bingdong Sha
  • Lena Burri
  • Billy T Dye
  • A Hönlinger
  • Travis Beddoe
  • Tohru Yoshihisa
  • Jason C Young
  • Piotr Neumann
  • Thomas Liepold
  • Christian Schulz
  • Oleksandr Lytovchenko
  • Toshiya Endo
  • Olaf Jahn
  • Alexander Schendzielorz
  • Ridhima Gomkale
  • Ralf Ficner
  • Bernhard Schmidt
  • Markus Kilisch
  • Cory D Dunn
  • Nebibe Mutlu
  • Nathan A Lack
  • Hayashi Yamamoto
  • Malgorzata E Sztolsztener
  • P J Dekker
  • Maya Schuldiner
  • Martin Jung
  • Sophia Nina Koerdt
  • Dennis Worm
  • Karoline Leopold
  • Yael Elbaz-Alon
  • Agata Trojanowska
  • F Nora Vögtle
  • Piotr Bragoszewski
  • Silke Oeljeklaus
  • Nir Ben-Tal
  • Agnes Schulze-Specking
  • Ralf M Zerbes
  • Karina von der Malsburg
  • Kaye N Truscott
  • Alexej Kedrov
  • Tomasz Czerwik
  • Yana Gofman
  • Yoav Arava
  • Chen Lesnik
  • J Y Koh
  • Arnold J M Driessen
  • Inge Perschil
  • Martin van der Laan
  • Agnieszka Gornicka
  • Yechiel Shai

Detail Information

Publications42

  1. pmc Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex
    P J Dekker
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
    Mol Cell Biol 18:6515-24. 1998
    ..with a molecular weight of approximately 400,000 ( approximately 400K), while the other receptors, Tom20 and Tom70, are more loosely associated with this GIP complex and can be found in distinct subcomplexes...
  2. ncbi Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae
    Lena Burri
    Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Australia
    FEBS J 273:1507-15. 2006
    ..The alpha-helical outer membrane proteins provide functions derived after the endosymbiotic event, and require the translocase in the outer mitochondrial membrane complex for insertion into the outer membrane...
  3. ncbi Crystal structure of yeast mitochondrial outer membrane translocon member Tom70p
    Yunkun Wu
    Department of Cell Biology, Center for Biophysical Sciences and Engineering, MCLM 364, 1918 Univ Blvd, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
    Nat Struct Mol Biol 13:589-93. 2006
    ..b>Tom70 is a major surface receptor for mitochondrial protein precursors in the TOM complex...
  4. ncbi Biogenesis of the mitochondrial TOM complex: Mim1 promotes insertion and assembly of signal-anchored receptors
    Thomas Becker
    Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, D 79104 Freiburg, Germany
    J Biol Chem 283:120-7. 2008
    ..The three receptors Tom20, Tom22, and Tom70 are anchored in the outer membrane by a single transmembrane alpha-helix, located at the N terminus in the case of ..
  5. pmc The peripheral membrane subunits of the SAM complex function codependently in mitochondrial outer membrane biogenesis
    Nickie C Chan
    Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria 3010, Australia
    Mol Biol Cell 19:126-36. 2008
    ..Very different environments surround bacteria and mitochondria, and we discuss the role of Sam35 and Sam37 in terms of the problems peculiar to mitochondrial protein substrates...
  6. ncbi Molecular chaperones Hsp90 and Hsp70 deliver preproteins to the mitochondrial import receptor Tom70
    Jason C Young
    Cellular Biochemistry, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Cell 112:41-50. 2003
    ..in mammals, the cytosolic chaperones Hsp90 and Hsp70 dock onto a specialized TPR domain in the import receptor Tom70 at the outer mitochondrial membrane...
  7. pmc Tetratricopeptide repeat proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis
    Noriko Kondo-Okamoto
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112 5650, USA
    EMBO Rep 9:63-9. 2008
    ..Here, we show that two tetratricopeptide repeat proteins-the general preprotein import receptor Tom70 (a component of translocase of the outer membrane) and its paralogue Tom71-are required for Mfb1 mitochondrial ..
  8. pmc Mitochondrial cardiolipin involved in outer-membrane protein biogenesis: implications for Barth syndrome
    Natalia Gebert
    Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
    Curr Biol 19:2133-9. 2009
    ..Our findings reveal a new role for cardiolipin in protein sorting at the mitochondrial outer membrane and bear implications for the pathogenesis of Barth syndrome...
  9. doi Regulation of mitochondrial protein import by cytosolic kinases
    Oliver Schmidt
    Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, Freiburg, Germany
    Cell 144:227-39. 2011
    ..PKA phosphorylates Tom70 under nonrespiring conditions, thereby inhibiting its receptor activity and the import of mitochondrial metabolite ..
  10. pmc The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins
    Thomas Becker
    Institute for Biochemistry and Molecular Biology, Centre for Biochemistry and Molecular Cell Research, Freiburg, Germany
    J Cell Biol 194:387-95. 2011
    ..The Mim1 complex cooperates with the receptor Tom70 in binding of precursor proteins and promotes their insertion and assembly into the outer membrane...
  11. pmc Multispan mitochondrial outer membrane protein Ugo1 follows a unique Mim1-dependent import pathway
    Drazen Papic
    Interfaculty Institute of Biochemistry, University of Tubingen, Germany
    J Cell Biol 194:397-405. 2011
    ..Using a specific insertion assay and analysis by native gel electrophoresis, we show that the import receptor Tom70, but not its partner Tom20, is involved in the initial recognition of the Ugo1 precursor...
  12. pmc Biogenesis of the preprotein translocase of the outer mitochondrial membrane: protein kinase A phosphorylates the precursor of Tom40 and impairs its import
    Sanjana Rao
    Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, 79104 Freiburg, Germany
    Mol Biol Cell 23:1618-27. 2012
    ..The major subunits of the TOM complex are the three receptors Tom20, Tom22, and Tom70 and the central channel-forming protein Tom40...
  13. pmc In vivo evidence for cooperation of Mia40 and Erv1 in the oxidation of mitochondrial proteins
    Lena Böttinger
    Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, 79104 Freiburg, Germany
    Mol Biol Cell 23:3957-69. 2012
    ..Thus Mia40 in cooperation with Erv1 promotes the formation of two disulfide bonds in the substrate protein, ensuring the efficiency of oxidative folding in the intermembrane space of mitochondria...
  14. pmc Functional cooperation of mitochondrial protein import receptors in yeast
    L Ramage
    Biocenter, University of Basel, Switzerland
    EMBO J 12:4115-23. 1993
    ..contain the sequence motif F-X-K-A-L-X-V/L, which is repeated several times with minor variations in the MAS70/MOM72 receptors...
  15. ncbi Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria
    M Moczko
    Biochemisches Institut, Universitat Freiburg, Germany
    J Biol Chem 269:9045-51. 1994
    The mitochondrial outer membrane proteins MOM19 and MOM72 are thought to function as import receptors for nuclear encoded preproteins...
  16. pmc The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins
    A Hönlinger
    Biochemisches Institut, Universitat Freiburg, Germany
    Mol Cell Biol 15:3382-9. 1995
    ..even suppressed the lethal phenotype of a double deletion of the two import receptors known so far (mom19 delta mom72 delta)...
  17. pmc Import of ADP/ATP carrier into mitochondria: two receptors act in parallel
    H F Steger
    Institut fur Physiologische Chemie, Universitat Munchen, Federal Republic of Germany
    J Cell Biol 111:2353-63. 1990
    We have identified the yeast homologue of Neurospora crassa MOM72, the mitochondrial import receptor for the ADP/ATP carrier (AAC), by functional studies and by cDNA sequencing...
  18. doi A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria
    Jonathan Melin
    Institute of Cellular Biochemistry, University Medical Center Gottingen, Germany
    Biochim Biophys Acta 1853:1850-9. 2015
    ..Aside from the well-characterized presequence receptors Tom20 and Tom22 a third TOM receptor, Tom70, binds proteins of the carrier family containing multiple transmembrane segments...
  19. pmc Mitochondrial protein import: Mia40 facilitates Tim22 translocation into the inner membrane of mitochondria
    Lidia Wrobel
    International Institute of Molecular and Cell Biology, 02 109 Warsaw, Poland
    Mol Biol Cell 24:543-54. 2013
    ..We propose that Mia40 not only is responsible for disulfide bond formation, but also assists the Tim22 protein in its integration into the inner membrane of mitochondria...
  20. pmc Deletion of conserved protein phosphatases reverses defects associated with mitochondrial DNA damage in Saccharomyces cerevisiae
    Görkem Garipler
    Department of Molecular Biology and Genetics and School of Medicine, Koc University, Sariyer, Istanbul, 34450 Turkey
    Proc Natl Acad Sci U S A 111:1473-8. 2014
    ..Our work highlights the important role that nutrient-responsive signaling pathways can play in determining the response to mitochondrial dysfunction. ..
  21. pmc The role of Djp1 in import of the mitochondrial protein Mim1 demonstrates specificity between a cochaperone and its substrate protein
    Drazen Papic
    Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany
    Mol Cell Biol 33:4083-94. 2013
    ..Two interesting candidates were the cytosolic cochaperone Djp1 and the mitochondrial import receptor Tom70. Our results indeed demonstrate a direct interaction of newly synthesized Mim1 molecules with Tom70...
  22. pmc Processing and topology of the yeast mitochondrial phosphatidylserine decarboxylase 1
    Susanne E Horvath
    Institut fur Biochemie, Technische Universitat Graz, Petersgasse 12 2, A 8010 Graz, Austria
    J Biol Chem 287:36744-55. 2012
    ..In summary, membrane integration of Psd1 is crucial for its functionality and for maintenance of mitochondrial lipid homeostasis...
  23. pmc Role of mitochondrial inner membrane organizing system in protein biogenesis of the mitochondrial outer membrane
    Maria Bohnert
    Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
    Mol Biol Cell 23:3948-56. 2012
    ..We conclude that MINOS interacts with TOM and SAM independently and that the core subunit mitofilin is involved in biogenesis of outer membrane β-barrel proteins...
  24. doi Membrane integration of a mitochondrial signal-anchored protein does not require additional proteinaceous factors
    Elisa Merklinger
    Interfaculty Institute of Biochemistry, University of Tubingen, Hoppe Seyler Strasse 4, Tubingen, Germany
    Biochem J 442:381-9. 2012
    ..Collectively, our results suggest that SA proteins are specifically inserted into the MOM by a process that is not dependent on additional proteins, but is rather facilitated by the distinct lipid composition of this membrane...
  25. doi The protein chaperone Ssa1 affects mRNA localization to the mitochondria
    Erez Eliyahu
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    FEBS Lett 586:64-9. 2012
    ..Importantly, deletion of the mitochondrial receptor Tom70 abolished the impact of Ssa1 overexpression on mRNAs encoding Tom70 targets...
  26. ncbi The C-terminal TPR domain of Tom70 defines a family of mitochondrial protein import receptors found only in animals and fungi
    Nickie C Chan
    Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville 3010, Australia
    J Mol Biol 358:1010-22. 2006
    ..in the outer mitochondrial membrane (TOM complex) consists of multiple components including the receptor subunit Tom70. Genome sequence analyses suggest no Tom70 receptor subunit exists in plants or protozoans, raising questions ..
  27. ncbi Identification of the mitochondrial receptor complex in Saccharomyces cerevisiae
    M Moczko
    Institut fur Physiologische Chemie, Universitat Munchen, Germany
    FEBS Lett 310:265-8. 1992
    ..In Neurospora crassa, the two import receptors, MOM19 and MOM72, were found in a complex with the general insertion protein, GIP (formed by MOM7, MOM8, MOM30 and MOM38) and MOM22...
  28. pmc Mas37p, a novel receptor subunit for protein import into mitochondria
    S Gratzer
    Biozentrum, University of Basel, Switzerland
    J Cell Biol 129:25-34. 1995
    ....
  29. ncbi The use of chemical cross-linking to identify proteins that interact with a mitochondrial presequence
    A S Gaikwad
    Department of Molecular Biology and Biochemistry, University of California, Irvine 92717
    J Biol Chem 269:6437-43. 1994
    ....
  30. pmc The Mas20p and Mas70p subunits of the protein import receptor of yeast mitochondria interact via the tetratricopeptide repeat motif in Mas20p: evidence for a single hetero-oligomeric receptor
    V Haucke
    Biozentrum, University of Basel, Switzerland
    EMBO J 15:1231-7. 1996
    ..We conclude that the two receptor subcomplexes of the mitochondrial protein import receptor interact in vivo via their Mas20p and Mas70p subunits and that this interaction is functionally important...
  31. ncbi Tom5 functionally links mitochondrial preprotein receptors to the general import pore
    K Dietmeier
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Germany
    Nature 388:195-200. 1997
    ..Tom20-Tom22 and Tom70-Tom37 function as import receptors with a preference for preproteins that have amino-terminal presequences or ..
  32. ncbi The mitochondrial import receptor Tom70: identification of a 25 kDa core domain with a specific binding site for preproteins
    J Brix
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, Freiburg, D 79104, Germany
    J Mol Biol 303:479-88. 2000
    The mitochondrial import receptor of 70 kDa, Tom70, preferentially recognizes precursors of membrane proteins with internal targeting signals...
  33. ncbi Yeast mitochondrial dehydrogenases are associated in a supramolecular complex
    X Grandier-Vazeille
    UMR5095 C N R S Université de Bordeaux 2, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
    Biochemistry 40:9758-69. 2001
    ..The association of these proteins is discussed in terms of NADH-channeling...
  34. pmc Overproduction of PDR3 suppresses mitochondrial import defects associated with a TOM70 null mutation by increasing the expression of TOM72 in Saccharomyces cerevisiae
    J Y Koh
    Department of Microbiology, University of Alabama at Birmingham, 35294 2170, USA
    Mol Cell Biol 21:7576-86. 2001
    ..network, as a multicopy suppressor of the mitochondrial import defects associated with Delta 1,2 pre-F(1)beta in a tom70 Delta strain...
  35. doi Domain organization of the monomeric form of the Tom70 mitochondrial import receptor
    Ryan D Mills
    Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria, Australia
    J Mol Biol 388:1043-58. 2009
    b>Tom70 is a mitochondrial protein import receptor composed of 11 tetratricopeptide repeats (TPRs)...
  36. ncbi Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes
    Hayashi Yamamoto
    Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa ku, 464 8602, Nagoya, Japan
    Cell 111:519-28. 2002
    ..A translocation intermediate accumulated at the TOM complex is crosslinked to Tim50. We suggest that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the TIM23 complex..
  37. pmc Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria
    Tohru Yoshihisa
    Research Center for Materials Science, Nagoya University, Nagoya, 464 8602, Japan
    Mol Biol Cell 14:3266-79. 2003
    ..It is difficult to explain these results from the view of tRNA splicing in the nucleus. We rather propose a new possibility that tRNA splicing occurs on the mitochondrial surface in yeast...
  38. ncbi A biophysical analysis of the tetratricopeptide repeat-rich mitochondrial import receptor, Tom70, reveals an elongated monomer that is inherently flexible, unstable, and unfolds via a multistate pathway
    Travis Beddoe
    Protein Crystallography Unit, The ARC Centre for Structural and Functional Microbial Genomics, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia
    J Biol Chem 279:46448-54. 2004
    ..One receptor, Tom70, binds large, hydrophobic mitochondrial precursors...
  39. ncbi Detecting protein-protein interaction in live yeast by flow cytometry
    Billy T Dye
    Howard Hughes Medical Institute, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
    Cytometry A 63:77-86. 2005
    ..Probability binning is also employed to provide a statistical confirmation of our observations...
  40. pmc Preliminary X-ray crystallographic studies of yeast mitochondrial protein Tom70p
    Yunkun Wu
    Department of Cell Biology, Center for Biophysical Sciences and Engineering, University of Alabama, Birmingham, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:265-7. 2006
    ..78, c = 83.41 A, alpha = 90.00, beta = 102.74, gamma = 90.00 degrees. There are two Tom70p molecules in one asymmetric unit, which corresponds to a solvent content of approximately 51%. Structure determination by MAD methods is under way...
  41. ncbi Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22, and Tom70
    J Brix
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
    J Biol Chem 272:20730-5. 1997
    ..We have expressed and purified the cytosolic domains of three postulated import receptors, Tom20, Tom22, and Tom70. Each receptor domain is able to bind mitochondrial preproteins but with different specificity...
  42. ncbi Insertion of hydrophobic membrane proteins into the inner mitochondrial membrane--a guided tour
    Peter Rehling
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Str 7, D 79104 Freiburg, Germany
    J Mol Biol 326:639-57. 2003
    ..The identification of components of these complexes and the analysis of the molecular mechanisms underlying their function are currently an exciting and fast developing field of molecular cell biology...