TOF1

Summary

Gene Symbol: TOF1
Description: Tof1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Post-replication repair suppresses duplication-mediated genome instability
    Christopher D Putnam
    Ludwig Institute for Cancer Research, University of California San Diego School of Medicine, La Jolla, California, United States of America
    PLoS Genet 6:e1000933. 2010
  2. ncbi S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex
    Yuki Katou
    Genome Structure and Function Team, Human Genome Research Group, RIKEN Genomic Science Center, 1 7 22 Suehiro cho, Japan
    Nature 424:1078-83. 2003
  3. pmc Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks
    Masashige Bando
    Department of Biological Science, Laboratory of Chromosome Structure and Function, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B 20, 4259, Nagatsuta, Midori ku, Yokohama City, Kanagawa, 226 8501, Japan
    J Biol Chem 284:34355-65. 2009
  4. doi Ctf4 coordinates the progression of helicase and DNA polymerase alpha
    Hirokazu Tanaka
    Laboratory of Chromosome Structure and Function, Department of Biological Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama City, Kanagawa, Japan
    Genes Cells 14:807-20. 2009
  5. ncbi Hst3 is regulated by Mec1-dependent proteolysis and controls the S phase checkpoint and sister chromatid cohesion by deacetylating histone H3 at lysine 56
    Safia Thaminy
    Clinical Research Division and Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Biol Chem 282:37805-14. 2007
  6. pmc Genetic dissection of parallel sister-chromatid cohesion pathways
    Hong Xu
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Genetics 176:1417-29. 2007
  7. pmc Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork
    Arturo Calzada
    Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK
    Genes Dev 19:1905-19. 2005
  8. ncbi Mrc1 and Tof1 promote replication fork progression and recovery independently of Rad53
    Hélène Tourrière
    Institute of Human Genetics, Centre National de la Recherche Scientifique, UPR 1142, 34396 Montpellier Cedex 5, France
    Mol Cell 19:699-706. 2005
  9. pmc Identification of protein complexes required for efficient sister chromatid cohesion
    Melanie L Mayer
    Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4
    Mol Biol Cell 15:1736-45. 2004
  10. pmc Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest
    Deepak Bastia
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29415
    Proc Natl Acad Sci U S A 113:E3639-48. 2016

Research Grants

  1. MECHANISM OF REPLICATION OF DRUG RESISTANCE FACTORS
    Deepak Bastia; Fiscal Year: 2008
  2. MECHANISM OF TERMINATION OF DNA REPLICATION
    Deepak Bastia; Fiscal Year: 2010

Scientific Experts

  • Deepak Bastia
  • Linda J Reha-Krantz
  • Bidyut K Mohanty
  • E J Foss
  • Masashige Bando
  • Christopher D Putnam
  • Kaushlendra Tripathi
  • Hirokazu Tanaka
  • Katsuhiko Shirahige
  • Yuki Katou
  • Makiko Komata
  • Stephanie A Schalbetter
  • Jun Che
  • Demis Menolfi
  • Ben Hodgson
  • Hong Xu
  • Karim Labib
  • Hajime Murakami
  • Sonya Dimitrova Uzunova
  • Arturo Calzada
  • Chi Meng Fong
  • Andrea R Leclere
  • Grant W Brown
  • Charles Boone
  • Vanessa Borges
  • Richard D Kolodner
  • Tikvah K Hayes
  • Amaya Alzu
  • Michael Chang
  • Nabil Matmati
  • Narendra K Bairwa
  • Tracey J Au
  • Xin Chenglin Li
  • Nicole Cherng
  • Philippe Pasero
  • Sergei M Mirkin
  • Maria L Naylor
  • Takashi Ohuchi
  • Takehiko Itoh
  • Hiroyuki Araki
  • Irina Voineagu
  • David F Razidlo
  • Safia Thaminy
  • Nathalie Grandin
  • Ashby J Morrison
  • Xuetong Shen
  • Jung Ae Kim
  • Nevan J Krogan
  • James E Haber
  • Jodie K Chin
  • Agnieszka Gambus
  • Christophe Redon
  • Michael Christopher Keogh
  • Masato Kanemaki
  • Floyd E Romesberg
  • Hélène Tourrière
  • Anandi S Karumbati
  • Marina N Nedelcheva
  • Bryan M O'Neill
  • Melanie L Mayer
  • Sahar Mansoubi
  • Eun Yong Shim
  • Stephanie Smith
  • Axel Delamarre
  • Sang Eun Lee
  • Dana Branzei
  • Yoo Jung Kim
  • Jessica A Downs
  • Anna L Chambers
  • Armelle Lengronne
  • Jonathan Baxter
  • Kyungjae Myung
  • Stoyno Stefanov Stoynov
  • Katielee Pallis
  • Scott Keeney
  • Alexander Stefanov Zarkov
  • Anna Marianova Ivanova
  • Marina Nedelcheva Nedelcheva-Veleva
  • Duncan J Smith
  • Ashwini Arumugam
  • Iestyn Whitehouse
  • Deanna M Koepp
  • Frank Uhlmann
  • David T Kirkpatrick
  • John K Yang
  • Andrea Cocito
  • Rodrigo Bermejo
  • Marco Foiani
  • Marco Saponaro
  • Chiara Lucca

Detail Information

Publications47

  1. pmc Post-replication repair suppresses duplication-mediated genome instability
    Christopher D Putnam
    Ludwig Institute for Cancer Research, University of California San Diego School of Medicine, La Jolla, California, United States of America
    PLoS Genet 6:e1000933. 2010
    ..Our analysis is consistent with models in which PRR prevents replication damage from becoming double strand breaks (DSBs) and/or regulates the activity of HR on DSBs...
  2. ncbi S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex
    Yuki Katou
    Genome Structure and Function Team, Human Genome Research Group, RIKEN Genomic Science Center, 1 7 22 Suehiro cho, Japan
    Nature 424:1078-83. 2003
    ..Here we demonstrate that the checkpoint regulatory proteins Tof1 and Mrc1 interact directly with the DNA replication machinery in Saccharomyces cerevisiae...
  3. pmc Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks
    Masashige Bando
    Department of Biological Science, Laboratory of Chromosome Structure and Function, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B 20, 4259, Nagatsuta, Midori ku, Yokohama City, Kanagawa, 226 8501, Japan
    J Biol Chem 284:34355-65. 2009
    Mrc1 (mediator of replication checkpoint), Tof1 (topoisomerase I interacting factor), and Csm3 (chromosome segregation in meiosis) are checkpoint-mediator proteins that function during DNA replication and activate the effector kinase ..
  4. doi Ctf4 coordinates the progression of helicase and DNA polymerase alpha
    Hirokazu Tanaka
    Laboratory of Chromosome Structure and Function, Department of Biological Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama City, Kanagawa, Japan
    Genes Cells 14:807-20. 2009
    ..The same interaction was not affected in the absence of the replication checkpoint mediators Tof1 or Mrc1...
  5. ncbi Hst3 is regulated by Mec1-dependent proteolysis and controls the S phase checkpoint and sister chromatid cohesion by deacetylating histone H3 at lysine 56
    Safia Thaminy
    Clinical Research Division and Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Biol Chem 282:37805-14. 2007
    ..Both S phase checkpoint and SCC defects are phenocopied by H3K56 point mutants. Our findings demonstrate that Hst3-regulated H3K56 acetylation safeguards genome stability by controlling the S phase DNA damage response and promoting SCC...
  6. pmc Genetic dissection of parallel sister-chromatid cohesion pathways
    Hong Xu
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Genetics 176:1417-29. 2007
    ..These data defined two cohesion pathways, one containing CSM3, TOF1, CTF4, and CHL1, and the second containing MRC1, CTF18, CTF8, and DCC1...
  7. pmc Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork
    Arturo Calzada
    Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK
    Genes Dev 19:1905-19. 2005
    ..The budding yeast proteins Mrc1 and Tof1 associate with the putative MCM-Cdc45 helicase and limit progression of the replisome when nucleotides are depleted,..
  8. ncbi Mrc1 and Tof1 promote replication fork progression and recovery independently of Rad53
    Hélène Tourrière
    Institute of Human Genetics, Centre National de la Recherche Scientifique, UPR 1142, 34396 Montpellier Cedex 5, France
    Mol Cell 19:699-706. 2005
    ..The critical role of Mrc1p in HU is therefore to promote fork recovery in a Rad53p-independent manner, presumably through the formation of a stable fork-pausing complex...
  9. pmc Identification of protein complexes required for efficient sister chromatid cohesion
    Melanie L Mayer
    Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4
    Mol Biol Cell 15:1736-45. 2004
    ..Deletion of seven genes (CHL1, CSM3, BIM1, KAR3, TOF1, CTF4, and VIK1) resulted in defective sister chromatid cohesion...
  10. pmc Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest
    Deepak Bastia
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29415
    Proc Natl Acad Sci U S A 113:E3639-48. 2016
    ..However, a general mechanism of PFA has remained elusive. We previously showed that the Tof1-Csm3 fork protection complex is essential for PFA by antagonizing the Rrm3 helicase that displaces nonhistone ..
  11. pmc DNA repair pathway selection caused by defects in TEL1, SAE2, and de novo telomere addition generates specific chromosomal rearrangement signatures
    Christopher D Putnam
    Ludwig Institute for Cancer Research, University of California School of Medicine, San Diego, La Jolla, California, United States of America Department of Medicine, University of California School of Medicine, San Diego, La Jolla, California, United States of America
    PLoS Genet 10:e1004277. 2014
    ..retaining a chrV L telomeric hph marker was significantly higher in tel1Δ, sae2Δ, rad53Δ sml1Δ, and mrc1Δ tof1Δ mutants...
  12. pmc Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes
    Hajime Murakami
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    Cell 158:861-73. 2014
    ..We now show that, in yeast, the replisome-associated components Tof1 and Csm3 physically associate with the Dbf4-dependent Cdc7 kinase (DDK) and recruit it to the replisome, where it ..
  13. pmc Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance
    Demis Menolfi
    IFOM, The FIRC Institute of Molecular Oncology, Via Adamello 16, 20139, Milan, Italy
    Mol Cell 60:835-46. 2015
    ..chromosome fragility and toxic recombination instigated by prolonged pausing and the fork protection complex, Tof1-Csm3...
  14. pmc Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability
    Stephanie A Schalbetter
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, East Sussex BN1 9RQ, United Kingdom
    Proc Natl Acad Sci U S A 112:E4565-70. 2015
    ..However, generally, fork rotation and precatenation are actively inhibited by Timeless/Tof1 and Tipin/Csm3...
  15. pmc The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence
    Sonya Dimitrova Uzunova
    Institute of Molecular Biology Roumen Tsanev, Bulgarian Academy of Sciences, 21 Acad George Bonchev Str, 1113 Sofia, Bulgaria
    Cell Div 9:4. 2014
    ..The S. cerevisiae complex Tof1/Csm3/Mrc1 acts to restrain the replicative MCM helicase when DNA synthesis is prohibited...
  16. pmc Hyper-Acetylation of Histone H3K56 Limits Break-Induced Replication by Inhibiting Extensive Repair Synthesis
    Jun Che
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America Program of Radiation Biology, Department of Radiation Oncology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America
    PLoS Genet 11:e1004990. 2015
    ..Our studies suggest that acetylation of H3K56 limits extensive repair synthesis and interferes with efficient fork progression in BIR. ..
  17. pmc An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae
    Vanessa Borges
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Chromosoma 122:121-34. 2013
    ..In addition to Eco1, several other factors contribute to cohesion establishment, including Ctf4, Ctf18, Tof1, Csm3, Chl1 and Mrc1, but little is known about their roles...
  18. pmc The direct binding of Mrc1, a checkpoint mediator, to Mcm6, a replication helicase, is essential for the replication checkpoint against methyl methanesulfonate-induced stress
    Makiko Komata
    Laboratory of Chromosome Structure and Function, Department of Biological Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama City, Kanagawa, Japan
    Mol Cell Biol 29:5008-19. 2009
    ..These results strongly suggest for the first time that an Mcm helicase acts as a checkpoint sensor for methyl methanesulfonate-induced DNA damage through direct binding to the replication checkpoint mediator Mrc1...
  19. pmc Drug-sensitive DNA polymerase δ reveals a role for mismatch repair in checkpoint activation in yeast
    Linda J Reha-Krantz
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
    Genetics 189:1211-24. 2011
    ....
  20. doi Nicotinamide induces Fob1-dependent plasmid integration into chromosome XII in Saccharomyces cerevisiae
    Kaushlendra Tripathi
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, USA
    FEMS Yeast Res 12:949-57. 2012
    ..This study suggests that NAD metabolism and intracellular NAM concentrations are important in Fob1-mediated rDNA recombination...
  21. pmc Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes
    Amaya Alzu
    The FIRC Institute of Molecular Oncology IFOM Foundation, Via Adamello 16, 20139 Milan, Italy
    Cell 151:835-46. 2012
    ....
  22. pmc The role of CSM3, MRC1, and TOF1 in minisatellite stability and large loop DNA repair during meiosis in yeast
    Andrea R Leclere
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, United States
    Fungal Genet Biol 50:33-43. 2013
    ..Here we show that minisatellite length expansions are controlled by the products of the CSM3 and TOF1 genes, while contractions are controlled by MRC1...
  23. pmc The Saccharomyces cerevisiae F-box protein Dia2 is a mediator of S-phase checkpoint recovery from DNA damage
    Chi Meng Fong
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Genetics 193:483-99. 2013
    ..We propose a model in which Dia2 mediates Mrc1 degradation to help cells resume the cell cycle during recovery from MMS-induced DNA damage in S-phase...
  24. pmc ATP-dependent chromatin remodeling factors tune S phase checkpoint activity
    Tracey J Au
    Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell Biol 31:4454-63. 2011
    ..Based on these results, we propose that Isw2 and Ino80 are targeted to stalled replication forks via RPA and directly control the amplitude of S phase checkpoint activity and the subsequent deactivation process...
  25. pmc Cellular morphogenesis under stress is influenced by the sphingolipid pathway gene ISC1 and DNA integrity checkpoint genes in Saccharomyces cerevisiae
    Kaushlendra Tripathi
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Genetics 189:533-47. 2011
    ..That is, yeast carrying deletions of both ISC1 and a replication checkpoint mediator gene including MRC1, TOF1, or CSM3 display basal morphological defects, which increase following HU treatment...
  26. pmc Expansions, contractions, and fragility of the spinocerebellar ataxia type 10 pentanucleotide repeat in yeast
    Nicole Cherng
    Department of Biology, Tufts University, Medford, MA 02155, USA
    Proc Natl Acad Sci U S A 108:2843-8. 2011
    ..A first round of genetic analysis showed that a functional TOF1 gene precludes, whereas a functional RAD5 gene promotes, expansions of the (ATTCT)(n) repeat...
  27. pmc Ploidy dictates repair pathway choice under DNA replication stress
    Xin Chenglin Li
    Department of Molecular Biology and Genetics, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York 14853, USA
    Genetics 187:1031-40. 2011
    ..In response to replication stress, early events associated with ploidy dictate the repair pathway choice. This study uncovers a fundamental difference between haplophase and diplophase in the maintenance of genome integrity...
  28. pmc The intra-S phase checkpoint protein Tof1 collaborates with the helicase Rrm3 and the F-box protein Dia2 to maintain genome stability in Saccharomyces cerevisiae
    Narendra K Bairwa
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 286:2445-54. 2011
    The intra-S phase checkpoint protein complex Tof1/Csm3 of Saccharomyces cerevisiae antagonizes Rrm3 helicase to modulate replication fork arrest not only at the replication termini of rDNA but also at strong nonhistone protein binding ..
  29. doi Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication
    Takashi Ohuchi
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    DNA Repair (Amst) 8:690-6. 2009
    ..Double mutation of RAD51 and RAD53 exhibited the similar levels of Rad52 sumoylation to RAD53 single mutation. The significance and regulation mechanism of Rad52 sumoylation by checkpoint pathways will be discussed...
  30. ncbi Identification and characterization of the genes for two topoisomerase I-interacting proteins from Saccharomyces cerevisiae
    H Park
    Department of Biochemistry and Cell Biology, SUNY at Stony Brook 11794 5215, USA
    Yeast 15:35-41. 1999
    ..Two new genes were found and named TOF1 and TOF2. The Tof1 protein has 1238 amino acids and no obvious homologues in databases...
  31. pmc Tof1p regulates DNA damage responses during S phase in Saccharomyces cerevisiae
    E J Foss
    Division of Basic Sciences, A3 023, Fred Hutchinson Cancer Research Center, 1100 Faiorview Ave, Seattle, WA 98109 1024, USA
    Genetics 157:567-77. 2001
    A tof1 mutant was recovered in a screen aimed at identifying genes involved specifically in the S phase branch of the MEC1-dependent DNA damage response pathway...
  32. pmc Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response
    Bryan M O'Neill
    Department of Chemistry, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Nucleic Acids Res 32:6519-30. 2004
    ..A yeast two-hybrid screen identified a potential physical interaction between Wss1 and both Psy2 and Tof1. Genetic interactions were also detected between PSY2 and TOF1, as well as between each gene and RAD52 and SRS2, ..
  33. pmc Abrogation of the Chk1-Pds1 checkpoint leads to tolerance of persistent single-strand breaks in Saccharomyces cerevisiae
    Anandi S Karumbati
    Department of Pathology, University of Michigan Medical School, Ann Arbor, 48109 0602, USA
    Genetics 169:1833-44. 2005
    ..We propose a model in which recombinational repair during S phase coupled with failure of the metaphase-anaphase checkpoint allows for tolerance of persistent single-strand breaks at the expense of genome stability...
  34. ncbi Uncoupling of unwinding from DNA synthesis implies regulation of MCM helicase by Tof1/Mrc1/Csm3 checkpoint complex
    Marina N Nedelcheva
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria
    J Mol Biol 347:509-21. 2005
    ..In concordance with this suggestion, we found that the Tof1/Csm3/Mrc1 checkpoint complex interacts directly with the MCM helicase during both replication fork progression and ..
  35. pmc RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex
    Michael Chang
    Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
    EMBO J 24:2024-33. 2005
    ..In addition, rmi1Delta strains fail to fully activate Rad53 upon exposure to DNA-damaging agents, suggesting that Rmi1 is also an important part of the Rad53-dependent DNA damage response...
  36. pmc Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damage
    Christophe Redon
    NIH, NCI, DBS, Laboratory of Molecular Pharmacology, Bethesda, Maryland 20892, USA
    Genetics 172:67-76. 2006
    ..We now report that efficient repair also requires proteins involved in chromatid cohesion: Csm3; Tof1; Mrc1, and Dcc1. Epistasis analysis defined several pathways involving these proteins...
  37. ncbi A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 439:497-501. 2006
    ..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint...
  38. pmc The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae
    Bidyut K Mohanty
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Proc Natl Acad Sci U S A 103:897-902. 2006
    ..Simultaneous deletions of the TOF1 (or CSM3), and the RRM3 genes restored fork arrest by removing both the fork-releasing and fork-protection ..
  39. pmc Esc4/Rtt107 and the control of recombination during replication
    Jodie K Chin
    Department of Chemistry, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    DNA Repair (Amst) 5:618-28. 2006
    ..Similar behavior is also observed in the absence of damage when either of the S-phase checkpoint proteins, Tof1 or Mrc1, is deleted...
  40. ncbi Mrc1, a non-essential DNA replication protein, is required for telomere end protection following loss of capping by Cdc13, Yku or telomerase
    Nathalie Grandin
    UMR CNRS no 5161, Ecole Normale Superieure de Lyon, IFR128 BioSciences Gerland, 46, Allee d Italie, 69364 Lyon, France
    Mol Genet Genomics 277:685-99. 2007
    ..Combinations between the cdc13-1 mutation and deletion of either TOF1 or PSY2, coding for proteins physically interacting with Mrc1, also resulted in a synthetic growth defect...
  41. pmc Mrc1 and Tof1 regulate DNA replication forks in different ways during normal S phase
    Ben Hodgson
    Cancer Research U K, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom
    Mol Biol Cell 18:3894-902. 2007
    The Mrc1 and Tof1 proteins are conserved throughout evolution, and in budding yeast they are known to associate with the MCM helicase and regulate the progression of DNA replication forks...
  42. pmc Mrc1, Tof1 and Csm3 inhibit CAG.CTG repeat instability by at least two mechanisms
    David F Razidlo
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Box 986805, Omaha, NE 68198 6805, USA
    DNA Repair (Amst) 7:633-40. 2008
    ..Genetic analysis showed that Mrc1, along with Tof1 and Csm3, inhibits instability in two distinct ways...
  43. pmc Replication stalling at unstable inverted repeats: interplay between DNA hairpins and fork stabilizing proteins
    Irina Voineagu
    Department of Biology, Tufts University, Medford, MA 02155, USA
    Proc Natl Acad Sci U S A 105:9936-41. 2008
    ..Finally, we report that yeast fork-stabilizing proteins, Tof1 and Mrc1, are required to counteract repeat-mediated replication stalling...
  44. pmc Contrasting roles of checkpoint proteins as recombination modulators at Fob1-Ter complexes with or without fork arrest
    Bidyut K Mohanty
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Eukaryot Cell 8:487-95. 2009
    ..The checkpoint adapter proteins Tof1 and Csm3 either positively or negatively controlled recombination depending on whether it was provoked by polar ..
  45. pmc Mrc1 phosphorylation in response to DNA replication stress is required for Mec1 accumulation at the stalled fork
    Maria L Naylor
    Department of Genetics, Harvard Medical School, Division of Genetics, Brigham and Women s Hospital, Howard Hughes Medical Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:12765-70. 2009
    ..Together these results reveal new insight into how Mrc1 facilitates checkpoint signal amplification at stalled replication forks...
  46. ncbi Mec1/Tel1 phosphorylation of the INO80 chromatin remodeling complex influences DNA damage checkpoint responses
    Ashby J Morrison
    Department of Carcinogenesis, Science Park Research Division, University of Texas M D Anderson Cancer Center, Smithville, TX 78957, USA
    Cell 130:499-511. 2007
    ..mutants are linked to the function of checkpoint regulators, such as the replication checkpoint factors Tof1 and Rad53...
  47. ncbi GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks
    Agnieszka Gambus
    Cancer Research UK, Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK
    Nat Cell Biol 8:358-66. 2006
    ..RPC components include the essential initiation and elongation factor, Cdc45, the checkpoint mediator Mrc1, the Tof1-Csm3 complex that allows replication forks to pause at protein-DNA barriers, the histone chaperone FACT (..

Research Grants2

  1. MECHANISM OF REPLICATION OF DRUG RESISTANCE FACTORS
    Deepak Bastia; Fiscal Year: 2008
    ..abstract_text> ..
  2. MECHANISM OF TERMINATION OF DNA REPLICATION
    Deepak Bastia; Fiscal Year: 2010
    ..Finally, we wish to uncover the mode of action of the Swi1 and Swi3 proteins to determine if these act as "antisweepases". ..