STE20

Summary

Gene Symbol: STE20
Description: mitogen-activated protein kinase kinase kinase kinase STE20
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. ncbi Pheromone response in yeast: association of Bem1p with proteins of the MAP kinase cascade and actin
    T Leeuw
    Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    Science 270:1210-3. 1995
  2. pmc A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia Unit, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany
    PLoS Biol 11:e1001495. 2013
  3. pmc Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex
    Lukasz Kozubowski
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 18:1719-26. 2008
  4. ncbi Sterile 20 kinase phosphorylates histone H2B at serine 10 during hydrogen peroxide-induced apoptosis in S. cerevisiae
    Sung Hee Ahn
    Laboratory of Chromatin Biology, The Rockefeller University, Box 78, New York, NY 10021, USA
    Cell 120:25-36. 2005
  5. pmc Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2
    Thomas Höfken
    The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Rd, Manchester, M20 4BX, UK
    J Cell Biol 164:219-31. 2004
  6. pmc A role for cell polarity proteins in mitotic exit
    Thomas Höfken
    The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK
    EMBO J 21:4851-62. 2002
  7. pmc A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae
    L Ni
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Mol Biol Cell 12:2147-70. 2001
  8. pmc Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway
    D C Raitt
    Dana Farber Cancer Institute, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 19:4623-31. 2000
  9. pmc PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae
    S P Holly
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 147:845-56. 1999
  10. pmc The MAPKKK Ste11 regulates vegetative growth through a kinase cascade of shared signaling components
    B N Lee
    Department of Biological Chemistry, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 96:12679-84. 1999

Research Grants

  1. GENETIC REGULATION OF YEAST CELL TYPE
    GEORGE SPRAGUE; Fiscal Year: 2008

Scientific Experts

  • Robert B Annan
  • GEORGE SPRAGUE
  • P M Pryciak
  • Meghal Gandhi
  • S Bhattacharjya
  • Thomas Höfken
  • A Tonouchi
  • April L Harkins
  • Meng Lin
  • Junwon Kim
  • Maryna Gorelik
  • E Leberer
  • C Wu
  • Katsuyoshi Yamamoto
  • Ursula Just
  • Gislene Pereira
  • Kazuo Tatebayashi
  • D Y Thomas
  • H U Mösch
  • T Leeuw
  • Franz Meitinger
  • Dennis Pultz
  • Alan R Davidson
  • Samyabrata Bhaduri
  • Je Hyun Yoon
  • Hao Wu
  • Tomoyuki Takaku
  • Clinton R Bartholomew
  • T J Richman
  • Keiichiro Tanaka
  • Haruo Saito
  • Wei Niu
  • Lukasz Kozubowski
  • Alicia G Howe
  • Christopher Tiedje
  • C Patrick Lusk
  • Melanie Heinrich
  • Corinne E Zeller
  • Satoe Takahashi
  • Yoshihiro Yamaguchi
  • M Whiteway
  • David Y Thomas
  • M Peter
  • Malcolm Whiteway
  • Sung Hee Ahn
  • Paul J Cullen
  • Tamara J Richman
  • April S Goehring
  • S P Holly
  • Josee Ash
  • Megan E Keniry
  • D I Johnson
  • Mark D Rose
  • Sean M O'Rourke
  • Astrid Winkler
  • Gregory R Smith
  • Rachel E Lamson
  • Birgit Hub
  • G R Fink
  • Heidi Richter
  • Sabrina Heisel
  • Wolfgang Seufert
  • A S Gladfelter
  • Sheena Claire Li
  • Christine Zimmermann
  • Nils J Færgeman
  • Martin V Bennetzen
  • Jens S Andersen
  • L Ni
  • Patricia M Kane
  • Jorrit M Enserink
  • D A Mitchell
  • R L Roberts
  • Steven Vestergaard Rødkær
  • M Snyder
  • Karen Stanger
  • Hiroyuki Kumeta
  • F Drogen
  • Y J Sheu
  • Roy Parker
  • Kenji Ogura
  • Patrick Brennwald
  • P J Cullen
  • Naoki Yoshida
  • Jimmy Gardner
  • Fuyuhiko Inagaki
  • V Reiser
  • G H Braus
  • Courtney Turner
  • D C Raitt

Detail Information

Publications86

  1. ncbi Pheromone response in yeast: association of Bem1p with proteins of the MAP kinase cascade and actin
    T Leeuw
    Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    Science 270:1210-3. 1995
    ..Thus, the association of Bem1p with Ste20p and Ste5p may contribute to the conveyance of spatial information that regulates polarized rearrangement of the actin cytoskeleton during yeast mating...
  2. pmc A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia Unit, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany
    PLoS Biol 11:e1001495. 2013
    ..Our findings provide unexpected insights into the temporal control of cytokinesis and describe the importance of a Gps1-dependent mechanism for highly accurate polarity switching between two closely connected locations...
  3. pmc Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex
    Lukasz Kozubowski
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 18:1719-26. 2008
    ..Cdc42p concentrates at a random cortical site during symmetry breaking in a manner that requires the scaffold protein Bem1p. The mechanism whereby Bem1p promotes this polarization was unknown...
  4. ncbi Sterile 20 kinase phosphorylates histone H2B at serine 10 during hydrogen peroxide-induced apoptosis in S. cerevisiae
    Sung Hee Ahn
    Laboratory of Chromatin Biology, The Rockefeller University, Box 78, New York, NY 10021, USA
    Cell 120:25-36. 2005
    ..b>Ste20 kinase, a yeast homolog of mammalian Mst1 kinase, translocates into the nucleus in a caspase-independent fashion ..
  5. pmc Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2
    Thomas Höfken
    The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Rd, Manchester, M20 4BX, UK
    J Cell Biol 164:219-31. 2004
    The guanine nucleotide exchange factor Cdc24, the GTPase Cdc42, and the Cdc42 effectors Cla4 and Ste20, two p21-activated kinases, form a signal transduction cascade that promotes mitotic exit in yeast...
  6. pmc A role for cell polarity proteins in mitotic exit
    Thomas Höfken
    The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK
    EMBO J 21:4851-62. 2002
    ..Moreover, Cdc24, Cdc42 and Ste20, another PAK, probably function parallel to Lte1 in facilitating mitotic exit...
  7. pmc A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae
    L Ni
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Mol Biol Cell 12:2147-70. 2001
    ..Genome-wide screens of defined collections of mutants hold significant promise for dissecting many biological processes in yeast...
  8. pmc Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway
    D C Raitt
    Dana Farber Cancer Institute, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 19:4623-31. 2000
    ..The yeast PAK homolog Ste20 is essential for the Sho1-dependent activation of the Hog1 MAP kinase in response to severe osmotic stress...
  9. pmc PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae
    S P Holly
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 147:845-56. 1999
    ..Inactivation of the PAK homologues Ste20 and Cla4 at various points in the cell cycle resulted in loss of cell and actin cytoskeletal polarity, but not in ..
  10. pmc The MAPKKK Ste11 regulates vegetative growth through a kinase cascade of shared signaling components
    B N Lee
    Department of Biological Chemistry, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 96:12679-84. 1999
    ..and invasive growth (IG) pathways use the same mitogen-activated protein kinase kinase kinase kinase (MAPKKKK, Ste20), MAPKKK (Ste11), MAPKK (Ste7), and transcription factor (Ste12) to promote either G(1) arrest and fusion or ..
  11. ncbi Actin cytoskeleton organization regulated by the PAK family of protein kinases
    J J Eby
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3202, USA
    Curr Biol 8:967-70. 1998
    ..Here, we show that mutants of the budding yeast Saccharomyces cerevisiae lacking the PAK homologs Ste20 and Cla4 exhibit actin cytoskeletal defects, in vivo and in vitro, that resemble those of cdc42-1 mutants...
  12. ncbi The phosphorylation site for Ste20p-like protein kinases is essential for the function of myosin-I in yeast
    C Wu
    Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2, Canada
    J Biol Chem 272:30623-6. 1997
    ..Our results suggest that phosphorylation of the head domain by Ste20p-like protein kinases plays an essential role in the function of myosin-I in yeast cells...
  13. ncbi Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast
    F Cvrckova
    Institute of Molecular Pathology IMP, Wien, Austria
    Genes Dev 9:1817-30. 1995
    The yeast Ste20 protein kinase is involved in pheromone response. Mammalian homologs of Ste20 exist, but their function remains unknown...
  14. ncbi Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae
    C Wu
    Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    J Biol Chem 270:15984-92. 1995
    ....
  15. ncbi Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth
    R L Roberts
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge
    Genes Dev 8:2974-85. 1994
    ..We find that the same components of the MAP kinase cascade necessary for diploid pseudohyphal development (STE20, STE11, STE7, and STE12) are also required for both filament formation and agar penetration in haploids...
  16. pmc A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae
    S W Ramer
    Department of Biochemistry, Stanford University School of Medicine, CA 94305
    Proc Natl Acad Sci U S A 90:452-6. 1993
    This work reports the identification, characterization, and nucleotide sequence of STE20, a newly discovered gene involved in the Saccharomyces cerevisiae mating response pathway, to date one of the best understood signal transduction ..
  17. pmc The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components
    E Leberer
    Biotechnology Research Institute, National Research Council of Canada
    EMBO J 11:4815-24. 1992
    ..We have identified a new gene, designated STE20, which encodes a protein kinase homologue with sequence similarity to protein kinase C, which is required to ..
  18. pmc Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling
    Junwon Kim
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Genet 11:e1005684. 2015
    ..Taken together, our findings show that budding yeast can access a stable constitutive pseudohyphal growth state with very few genetic and regulatory changes. ..
  19. pmc Polarized growth controls cell shape and bipolar bud site selection in Saccharomyces cerevisiae
    Y J Sheu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Mol Cell Biol 20:5235-47. 2000
    ..In addition, we found that the p21-activated kinase homologue Ste20 is also important for both apical growth and bipolar bud site selection...
  20. pmc p21-activated kinases Cla4 and Ste20 regulate vacuole inheritance in Saccharomyces cerevisiae
    Clinton R Bartholomew
    Department of Cell and Developmental Biology, Vanderbilt School of Medicine, Nashville, TN 37232 8240, USA
    Eukaryot Cell 8:560-72. 2009
    ..The p21-activated kinases (PAKs) Cla4 and Ste20 are localized to and activated in the bud...
  21. pmc A biochemical genomics screen for substrates of Ste20p kinase enables the in silico prediction of novel substrates
    Robert B Annan
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    PLoS ONE 4:e8279. 2009
    The Ste20/PAK family is involved in many cellular processes, including the regulation of actin-based cytoskeletal dynamics and the activation of MAPK signaling pathways...
  22. pmc A Conserved residue in the yeast Bem1p SH3 domain maintains the high level of binding specificity required for function
    Maryna Gorelik
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    J Biol Chem 286:19470-7. 2011
    ..This work provides a clear illustration of the importance of intrinsic binding specificity for the function of protein-protein interaction modules, and the key role of "negative" interactions in determining the specificity of a domain...
  23. pmc A third osmosensing branch in Saccharomyces cerevisiae requires the Msb2 protein and functions in parallel with the Sho1 branch
    Sean M O'Rourke
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143 0448, USA
    Mol Cell Biol 22:4739-49. 2002
    ..These observations indicate that Msb2 is partially redundant with the Sho1 osmosensing branch for the activation of Ste11...
  24. pmc POG1, a novel yeast gene, promotes recovery from pheromone arrest via the G1 cyclin CLN2
    M A Leza
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 151:531-43. 1999
    ..upregulation of the CLN2 gene and that the resulting Cln2 protein promotes recovery primarily through an effect on Ste20, an activator of the mating MAPK cascade...
  25. pmc Regulation of vacuolar H+-ATPase activity by the Cdc42 effector Ste20 in Saccharomyces cerevisiae
    Meng Lin
    Institute of Biochemistry, Christian Albrechts University, Kiel, Germany
    Eukaryot Cell 11:442-51. 2012
    In the budding yeast Saccharomyces cerevisiae, the Cdc42 effector Ste20 plays a crucial role in the regulation of filamentous growth, a response to nutrient limitation...
  26. pmc Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface
    Maryna Gorelik
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    J Biol Chem 287:9168-77. 2012
    ..To investigate this unusual behavior, we have solved the structure of the Nbp2p SH3-Ste20 peptide complex and compared it with the previously determined structure of the Bem1p SH3b bound to the same ..
  27. doi Global mapping of protein phosphorylation events identifies Ste20, Sch9 and the cell-cycle regulatory kinases Cdc28/Pho85 as mediators of fatty acid starvation responses in Saccharomyces cerevisiae
    Dennis Pultz
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark
    Mol Biosyst 8:796-803. 2012
    ..of these phosphorylation events, we have identified the cell cycle kinases Cdc28 and Pho85, the PAK kinase Ste20 as well as the protein kinase Sch9 as central mediators of the cellular response to inhibition of fatty acid ..
  28. pmc Genetic analysis of the interface between Cdc42p and the CRIB domain of Ste20p in Saccharomyces cerevisiae
    Josee Ash
    Genetics, National Research Council, Biotechnology Research Institute, Montreal, Quebec H4P 2R2, Canada
    Genetics 163:9-20. 2003
    ..These results suggest that the Ste20p-Cdc42p interaction plays a direct role in Ste20p kinase function and that this interaction is required for efficient activity of the pheromone response pathway...
  29. pmc Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in Saccharomyces cerevisiae
    Je Hyun Yoon
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
    J Cell Biol 189:813-27. 2010
    ..We show that during stress in Saccharomyces cerevisiae, Dcp2 is phosphorylated on serine 137 by the Ste20 kinase...
  30. ncbi Analysis of cell-cycle specific localization of the Rdi1p RhoGDI and the structural determinants required for Cdc42p membrane localization and clustering at sites of polarized growth
    Tamara J Richman
    Department of Microbiology and Molecular Genetics and the Markey Center for Molecular Genetics, University of Vermont, 95 Carrigan Dr, 202 Stafford Hall, Burlington, VT 05405, USA
    Curr Genet 45:339-49. 2004
    ..Taken together, these results provide insight into the complicated nature of the relationships between Cdc42p localization, nucleotide binding, and protein-protein interactions...
  31. pmc A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast
    Paul J Cullen
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Genes Dev 18:1695-708. 2004
    ..Taken together, our data suggest that Msb2 is a signaling mucin that interacts with general components, such as Cdc42 and Sho1, to promote their function in the FG pathway...
  32. ncbi Kin4 kinase delays mitotic exit in response to spindle alignment defects
    Gislene Pereira
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Mol Cell 19:209-21. 2005
    ..In response to spindle misalignment, Kin4 and Bub2-Bfa1 are brought together at both SPBs. Kin4 now maintains Bub2-Bfa1 activity by counteracting Cdc5, thereby inhibiting mitotic exit...
  33. pmc Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway
    Kazuo Tatebayashi
    Division of Molecular Cell Signaling, Institute of Medical Sciences, University of Tokyo, Tokyo, Japan
    EMBO J 25:3033-44. 2006
    ..We found that Cdc42, in addition to binding and activating the PAK-like kinases Ste20 and Cla4, binds to the Ste11-Ste50 complex to bring activated Ste20/Cla4 to their substrate Ste11...
  34. ncbi An NMR-based identification of a peptide fragment from the beta-subunit of a G-protein showing specific interactions with the GBB domain of the Ste20 kinase in budding yeast
    Surajit Bhattacharjya
    School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
    Biochem Biophys Res Commun 347:1145-50. 2006
    ..by interactions between the beta-subunit of a G-protein (G-beta) and the G-beta binding (GBB) domain of Ste20 kinase...
  35. ncbi A novel Cdc42-interacting domain of the yeast polarity establishment protein Bem1. Implications for modulation of mating pheromone signaling
    Yoshihiro Yamaguchi
    Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa 277 8561, Japan
    J Biol Chem 282:29-38. 2007
    ..interacts not only with Cdc42 but also with Cdc24 and the effectors of Cdc42, including the p21-activated kinase Ste20, to function as a scaffold for cell polarity establishment...
  36. pmc Role of Cdc42-Cla4 interaction in the pheromone response of Saccharomyces cerevisiae
    Melanie Heinrich
    Department of Biology, Molecular Genetics, Philipps University, D 35032 Marburg, Germany
    Eukaryot Cell 6:317-27. 2007
    ..controls the mitogen-activated protein (MAP) kinase cascade by activating the p21-activated protein kinase (PAK) Ste20. To further explore the role of Cdc42 in pheromone-stimulated signaling, we isolated novel alleles of CDC42 that ..
  37. ncbi Nup53p is a target of two mitotic kinases, Cdk1p and Hrr25p
    C Patrick Lusk
    Department of Cell Biology, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Traffic 8:647-60. 2007
    ..The ability of nup53 alleles containing Cdk1p site mutations to complement synthetic defects of nup53 Delta nup170 Delta strains is linked to a function for Nup53p in the spindle assembly checkpoint...
  38. ncbi The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast
    Corinne E Zeller
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:25168-76. 2007
    ..Our findings reveal the existence of an unusual Gbeta subunit, one having multiple functions within the cell in addition to serving as a signal transducer for cell surface receptors and intracellular effectors...
  39. pmc Identification of novel membrane-binding domains in multiple yeast Cdc42 effectors
    Satoe Takahashi
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 18:4945-56. 2007
    ..regulates polarity and mitogen-activated protein (MAP) kinase signaling in part through the PAK-family kinase Ste20. Activation of Ste20 requires a Cdc42/Rac interactive binding (CRIB) domain, which mediates its recruitment to ..
  40. ncbi An upstream regulator and downstream target of phospholipase D1 activity during pheromone response in Saccharomyces cerevisiae
    April L Harkins
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
    FEMS Yeast Res 8:237-44. 2008
    ..Finally, kinase activity assays provided evidence for the stimulation of Ste20p by PA. These findings highlight the important interactions among PLD1, Cdc42p and Ste20p during PCG in S. cerevisiae...
  41. pmc Cyclin-specific docking motifs promote phosphorylation of yeast signaling proteins by G1/S Cdk complexes
    Samyabrata Bhaduri
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Curr Biol 21:1615-23. 2011
    ..Two such proteins, Ste5 and Ste20, are phosphorylated only when Cdk is associated with the G1/S cyclins Cln1 and Cln2 and not G1, S, or M cyclins...
  42. pmc Mechanisms of cell cycle control revealed by a systematic and quantitative overexpression screen in S. cerevisiae
    Wei Niu
    Center for Systems and Synthetic Biology, University of Texas, Austin, Texas, United States of America
    PLoS Genet 4:e1000120. 2008
    ..This work thus implicates new genes in cell cycle progression, complements previous screens, and lays the foundation for future experiments to define more precisely roles for these genes in cell cycle progression...
  43. pmc Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations
    Junwon Kim
    Department of Biochemistry and Institute of Life Science and Biotechnology, Yonsei University, Seoul 120 749, Korea
    Mol Biol Cell 19:4328-40. 2008
    ..These findings demonstrate that there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles...
  44. pmc Pheromone signalling in Saccharomyces cerevisiae requires the small GTP-binding protein Cdc42p and its activator CDC24
    Z S Zhao
    Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge
    Mol Cell Biol 15:5246-57. 1995
    ..Epistatic analysis indicates that CDC24 and CDC42 lie between STE4 and STE20 in the pathway. The two-hybrid system revealed that Ste4p interacts with Cdc24p...
  45. pmc The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway
    Meng Lin
    Institute of Biochemistry, Christian Albrecht University, 24098 Kiel, Germany
    Mol Biol Cell 20:4826-37. 2009
    ..Rho-type GTPase Cdc42 regulates polarized growth through its effectors, including the p21-activated kinases (PAKs) Ste20, Cla4, and Skm1...
  46. pmc The Exo70 subunit of the exocyst is an effector for both Cdc42 and Rho3 function in polarized exocytosis
    Hao Wu
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7090, USA
    Mol Biol Cell 21:430-42. 2010
    ..These data suggest that interaction with the Exo70 component of the exocyst is a key event in spatial regulation of exocytosis by Rho GTPases...
  47. doi Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor
    Katsuyoshi Yamamoto
    Division of Molecular Cell Signaling, Institute of Medical Sciences, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Mol Cell 40:87-98. 2010
    ..Thus, dynamic regulation of Ste50-Opy2 interaction fine-tunes the MAPK signaling network...
  48. ncbi Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae
    Meng Lin
    Institute of Biochemistry, Christian Albrecht University, Kiel, Germany
    FEBS J 276:7253-64. 2009
    ..Here, we demonstrate that the deletion of either STE20 or CLA4 results in increased levels of sterols...
  49. pmc Solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42
    Tomoyuki Takaku
    Laboratory of Structural Biology, Graduate School of Life Science, Hokkaido University, N 12, W 6 Kita ku, Sapporo 060 0812, Japan
    J Biol Chem 285:19346-53. 2010
    ..Next, the solution structure of Bem1 SH3b-CI in complex with the proline-rich region of p21-activated kinase Ste20 (Ste20 PRR) was determined...
  50. pmc "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections
    C R Geyer
    The Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, CA 94704, USA
    Proc Natl Acad Sci U S A 96:8567-72. 1999
    ..Forward genetic analysis with peptide aptamer "mutagens" should be particularly useful in elucidating genetic networks in organisms and processes for which classical genetics is not feasible...
  51. pmc Loss of sustained Fus3p kinase activity and the G1 arrest response in cells expressing an inappropriate pheromone receptor
    A Couve
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, New York 10029, USA
    Mol Cell Biol 16:4478-85. 1996
    ....
  52. ncbi Genetic interactions indicate a role for Mdg1p and the SH3 domain protein Bem1p in linking the G-protein mediated yeast pheromone signalling pathway to regulators of cell polarity
    E Leberer
    Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada
    Mol Gen Genet 252:608-21. 1996
    ..The STE20 gene, encoding a protein kinase required for pheromone signal transduction, has recently been identified in a ..
  53. pmc Functional analysis of the interaction between the small GTP binding protein Cdc42 and the Ste20 protein kinase in yeast
    M Peter
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448, USA
    EMBO J 15:7046-59. 1996
    b>STE20 encodes a protein kinase related to mammalian p65Pak which functions in several signal transduction pathways in yeast, including those involved in pseudohyphal and invasive growth, as well as mating...
  54. ncbi Characterization of SKM1, a Saccharomyces cerevisiae gene encoding a novel Ste20/PAK-like protein kinase
    H Martin
    Departamento de Microbiologia II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain
    Mol Microbiol 23:431-44. 1997
    b>Ste20/PAK serine/threonine protein kinases have been suggested as playing essential roles in cell signalling and morphogenesis as potential targets of Cdc42 and Rac GTPases...
  55. ncbi 14-3-3 proteins are essential for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae
    R L Roberts
    Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Cambridge 02142, USA
    Cell 89:1055-65. 1997
    ..alleles of RAS2 and CDC42 induce pseudohyphal development and FG(TyA)-lacZ signaling in Bmh+ strains but not in ste20 (p65PAK) or bmh1 bmh2 mutant strains. Moreover, Bmh1p and Bmh2p associate with Ste20p in vivo...
  56. pmc Mating in Saccharomyces cerevisiae: the role of the pheromone signal transduction pathway in the chemotropic response to pheromone
    K Schrick
    Department of Genetics, University of Washington, Seattle 98195 7360, USA
    Genetics 147:19-32. 1997
    ..Cells mutant for components of the mitogen-activated protein (MAP) kinase cascade (ste5, ste20, ste11, ste7 or fus3 kss1) formed diploids at a frequency 1% that of the wild-type control, but formed prezygotes ..
  57. pmc AKR1 encodes a candidate effector of the G beta gamma complex in the Saccharomyces cerevisiae pheromone response pathway and contributes to control of both cell shape and signal transduction
    P M Pryciak
    Department of Genetics, University of Washington, Seattle 98195 7360, USA
    Mol Cell Biol 16:2614-26. 1996
    ....
  58. ncbi A screen for upstream components of the yeast protein kinase C signal transduction pathway identifies the product of the SLG1 gene
    J J Jacoby
    Institut fur Mikrobiologie, Heinrich Heine Universitat Dusseldorf, Germany
    Mol Gen Genet 258:148-55. 1998
    ..suppressed by overexpression of genes for other components of the Pkc pathway, such as PKC1, SLT2, ROM2, and STE20. In addition, a SLG1-lacZ reporter construct shows higher expression in the presence of caffeine or magnesium ..
  59. pmc The SLT2(MPK1) MAP kinase is activated during periods of polarized cell growth in yeast
    P Zarzov
    Departement de Biologie Cellulaire et Moleculaire, Centre d Etudes deSaclay, France
    EMBO J 15:83-91. 1996
    ..Upstream of BCK1(SLK1), we found that the STE20 kinase was required for SLT2 activation by mating pheromone, but was unnecessary for its activation during the ..
  60. ncbi Cell cycle- and Cln2p-Cdc28p-dependent phosphorylation of the yeast Ste20p protein kinase
    C Wu
    Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2
    J Biol Chem 273:28107-15. 1998
    Ste20p from Saccharomyces cerevisiae is a member of the Ste20/p21-activated protein kinase family of protein kinases...
  61. pmc Crosstalk between the Ras2p-controlled mitogen-activated protein kinase and cAMP pathways during invasive growth of Saccharomyces cerevisiae
    H U Mösch
    Institute for Microbiology and Genetics, Georg August University, D 37077 Gottingen, Germany
    Mol Biol Cell 10:1325-35. 1999
    ....
  62. ncbi The Cdc42p GTPase is involved in a G2/M morphogenetic checkpoint regulating the apical-isotropic switch and nuclear division in yeast
    T J Richman
    Department of Microbiology and Molecular Genetics and the Markey Center for Molecular Genetics, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 274:16861-70. 1999
    ....
  63. pmc Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway
    R Akada
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405, USA
    Genetics 143:103-17. 1996
    ..The other mutations were in STE5 and the STE20 kinase gene, which act near Ste4p in the pathway, and a new gene called STE21...
  64. ncbi Elements of the yeast pheromone response pathway required for filamentous growth of diploids
    H Liu
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142
    Science 262:1741-4. 1993
    ..Thus, a similar kinase cascade is activated by different signals in haploids and diploids and mediates different developmental outcomes in the two cell types...
  65. ncbi Phosphorylation of the MEKK Ste11p by the PAK-like kinase Ste20p is required for MAP kinase signaling in vivo
    F Drogen
    Swiss Institute for Experimental Cancer Research ISREC, Switzerland
    Curr Biol 10:630-9. 2000
    ..Genetic evidence suggests that the p21-activated protein kinase (PAK) Ste20p functions upstream of Ste11p, and Ste20p has been shown to phosphorylate Ste11p in vitro...
  66. pmc Defects in protein glycosylation cause SHO1-dependent activation of a STE12 signaling pathway in yeast
    P J Cullen
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Genetics 155:1005-18. 2000
    ..We specifically suggest that a Sho1 --> Ste20/Ste50 --> Ste11 --> Ste7 --> Kss1 --> Ste12 pathway is responsible for activation of FUS1 ..
  67. pmc GTPase-activating proteins for Cdc42
    Gregory R Smith
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Eukaryot Cell 1:469-80. 2002
    ..overproduction or loss of Rga1 and Rga2, but not Bem3, affected the two-hybrid interaction of Cdc42 with Ste20, a p21-activated kinase (PAK) kinase required for haploid invasive growth...
  68. pmc Heat stress activates the yeast high-osmolarity glycerol mitogen-activated protein kinase pathway, and protein tyrosine phosphatases are essential under heat stress
    Astrid Winkler
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA
    Eukaryot Cell 1:163-73. 2002
    ..Second, they block inappropriate cross talk between the HOG and the cell wall integrity MAPK pathways, suggesting that PTPs are important for maintaining specificity in MAPK signaling pathways...
  69. pmc Isolation and characterization of effector-loop mutants of CDC42 in yeast
    A S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Biol Cell 12:1239-55. 2001
    ..The availability of partial function alleles of CDC42 in a genetically tractable system serves as a useful starting point for genetic approaches to identify such novel effectors...
  70. ncbi Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1
    E Leberer
    Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    Mol Gen Genet 241:241-54. 1993
    ..A protein kinase homologue encoded by the STE20 gene has recently been identified as a potential G beta gamma target...
  71. ncbi Polarized localization of yeast Pbs2 depends on osmostress, the membrane protein Sho1 and Cdc42
    V Reiser
    Department of Biochemistry and Molecular Cell Biology, Ludwig Boltzmann Forschungsstelle, University of Vienna, Dr Bohrgasse 9, A1030, Vienna, Austria
    Nat Cell Biol 2:620-7. 2000
    ..Sho1 itself accumulates at sites of polar growth, but independently of stress conditions and Cdc42. These observations allow us to define the sequence of events that occurs during propogation of osmostress signals...
  72. pmc Saccharomyces cerevisiae cdc42p GTPase is involved in preventing the recurrence of bud emergence during the cell cycle
    T J Richman
    Department of Microbiology and Molecular Genetics and Markey Center for Molecular Genetics, University of Vermont Burlington, Vermont 05405, USA
    Mol Cell Biol 20:8548-59. 2000
    ....
  73. pmc The phosphotyrosyl phosphatase activator, Ncs1p (Rrd1p), functions with Cla4p to regulate the G(2)/M transition in Saccharomyces cerevisiae
    D A Mitchell
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Mol Cell Biol 21:488-500. 2001
    ..As for ste20 cla4-75 mutants, most ncs cla4-75 double mutants were defective for septin localization during budding...
  74. pmc Regulation of phosphoinositide levels by the phospholipid transfer protein Sec14p controls Cdc42p/p21-activated kinase-mediated cell cycle progression at cytokinesis
    Alicia G Howe
    Atlantic Research Centre, Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada
    Eukaryot Cell 6:1814-23. 2007
    ..4-kinases and phosphatidylinositol 4-phosphate 5-kinase prevented growth arrest by CDC42, CLA4, or STE20 upon inactivation of Sec14p function...
  75. pmc Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast
    April S Goehring
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Mol Biol Cell 14:4329-41. 2003
    ..Moreover, these five genes have a role in invasive growth and display genetic interactions with the TOR pathway. In summary, our results suggest the urmylation pathway is involved in nutrient sensing and budding...
  76. ncbi Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases
    T Leeuw
    Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    Nature 391:191-5. 1998
    Serine/threonine protein kinases of the Ste20/PAK family have been implicated in the signalling from heterotrimeric G proteins to mitogen-activated protein (MAP) kinase cascades...
  77. pmc Different domains of the essential GTPase Cdc42p required for growth and development of Saccharomyces cerevisiae
    H U Mösch
    Institute for Microbiology and Genetics, Georg August University, D 37077 Gottingen, Germany
    Mol Cell Biol 21:235-48. 2001
    ....
  78. pmc Accurate quantitation of protein expression and site-specific phosphorylation
    Y Oda
    The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 96:6591-6. 1999
    ..versus mutant cell populations and to the identification of in vivo phosphorylation sites in the PAK-related yeast Ste20 protein kinase that depend specifically on the G1 cyclin Cln2...
  79. pmc Rho5p is involved in mediating the osmotic stress response in Saccharomyces cerevisiae, and its activity is regulated via Msi1p and Npr1p by phosphorylation and ubiquitination
    Robert B Annan
    Department of Biochemistry, McIntyre Medical Building, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada
    Eukaryot Cell 7:1441-9. 2008
    ..These data identify Rho5p as being a target of Msi1p/Npr1p regulation and describe a regulatory circuit involving phosphorylation and ubiquitination...
  80. ncbi Proteins involved in sterol synthesis interact with Ste20 and regulate cell polarity
    Christopher Tiedje
    Institute of Biochemistry, Christian Albrecht University Kiel, Olshausenstrasse 40, 24098 Kiel, Germany
    J Cell Sci 120:3613-24. 2007
    The Saccharomyces cerevisiae p21-activated kinase (PAK) Ste20 regulates various aspects of cell polarity during vegetative growth, mating and filamentous growth...
  81. pmc Cdc42 regulation of kinase activity and signaling by the yeast p21-activated kinase Ste20
    Rachel E Lamson
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell Biol 22:2939-51. 2002
    The Saccharomyces cerevisiae kinase Ste20 is a member of the p21-activated kinase (PAK) family with several functions, including pheromone-responsive signal transduction...
  82. ncbi Role for the Rho-family GTPase Cdc42 in yeast mating-pheromone signal pathway
    M N Simon
    Scripps Research Institute, Department of Molecular Biology, La Jolla, California 92037, USA
    Nature 376:702-5. 1995
    ..But here we show that Cdc42 has a direct signalling role in the mating-pheromone response between the G protein and the downstream protein kinase cascade...
  83. ncbi Filamentous growth of the budding yeast Saccharomyces cerevisiae induced by overexpression of the WHi2 gene
    P A Radcliffe
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Microbiology 143:1867-76. 1997
    ..However, Whi2-induced filament formation is reduced, but not blocked, by mutations in STE7, STE12 or STE20 which do block pseudohypha formation...
  84. pmc Hsl7p, a negative regulator of Ste20p protein kinase in the Saccharomyces cerevisiae filamentous growth-signaling pathway
    A Fujita
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, 1 1 Higashi, Tsukuba 305 8566, Japan
    Proc Natl Acad Sci U S A 96:8522-7. 1999
    ..However, deletions of STE20 in haploid and diploid greatly diminished these hsl7-associated phenotypes...
  85. pmc Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase
    E Leberer
    Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    EMBO J 16:83-97. 1997
    ..These results suggest that Ste20p is regulated in different developmental pathways by different mechanisms which involve heterotrimeric and small GTP binding proteins...
  86. pmc Identification of p21-activated kinase specificity determinants in budding yeast: a single amino acid substitution imparts Ste20 specificity to Cla4
    Megan E Keniry
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Mol Cell Biol 23:1569-80. 2003
    Two closely related p21-activated kinases from Saccharomyces cerevisiae, Ste20 and Cla4, interact with and are regulated by Cdc42, a small Rho-like GTPase...

Research Grants1

  1. GENETIC REGULATION OF YEAST CELL TYPE
    GEORGE SPRAGUE; Fiscal Year: 2008
    ..b>Ste20, a p21-activated protein kinase that has human homologs, is one point of connection to the actin cytoskeleton...