SSA1

Summary

Gene Symbol: SSA1
Description: Hsp70 family ATPase SSA1
Alias: YG100
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. doi The Hsp70 chaperone Ssa1 is essential for catabolite induced degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase
    Jeannette Juretschke
    Institut fur Biochemie, Universitat Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany
    Biochem Biophys Res Commun 397:447-52. 2010
  2. pmc Isolation and characterization of extragenic suppressors of mutations in the SSA hsp70 genes of Saccharomyces cerevisiae
    R J Nelson
    Department of Physiological Chemistry, University of Wisconsin Madison 53706
    Genetics 131:277-85. 1992
  3. pmc Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae
    M Werner-Washburne
    Mol Cell Biol 7:2568-77. 1987
  4. pmc A heat shock transcription factor with reduced activity suppresses a yeast HSP70 mutant
    J T Halladay
    Department of Biomolecular Chemistry, University of Wisconsin, Madison 53706, USA
    Mol Cell Biol 15:4890-7. 1995
  5. pmc Functional interaction of cytosolic hsp70 and a DnaJ-related protein, Ydj1p, in protein translocation in vivo
    J Becker
    Department of Biomolecular Chemistry, University of Wisconsin Madison, 53706, USA
    Mol Cell Biol 16:4378-86. 1996
  6. pmc Members of the Hsp70 family of proteins in the cell wall of Saccharomyces cerevisiae
    J L Lopez-Ribot
    Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, 79430, USA
    J Bacteriol 178:4724-6. 1996
  7. pmc In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation
    N Shulga
    Department of Biology, University of Rochester, New York 14627, USA
    J Cell Biol 135:329-39. 1996
  8. ncbi Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins
    J R Glover
    Howard Hughes Medical Institute and Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA
    Cell 94:73-82. 1998
  9. ncbi Loss of Hsp70-Hsp40 chaperone activity causes abnormal nuclear distribution and aberrant microtubule formation in M-phase of Saccharomyces cerevisiae
    M Oka
    Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    J Biol Chem 273:29727-37. 1998
  10. ncbi A nuclear export signal prevents Saccharomyces cerevisiae Hsp70 Ssb1p from stimulating nuclear localization signal-directed nuclear transport
    N Shulga
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    J Biol Chem 274:16501-7. 1999

Scientific Experts

  • Do Hee Lee
  • H Saibil
  • Robert Phillip Smith
  • Mercedes Dosil
  • Joanna Krzewska
  • Jill L Johnson
  • KEVIN ANTHONY MORANO
  • Virginie Redeker
  • Yusuf Tutar
  • E A Craig
  • Daniel C Masison
  • Jeffrey L Brodsky
  • Robert Yung Liang Wang
  • Bernd Bukau
  • Claes Andréasson
  • Jingzhi Li
  • Patrick A Gibney
  • Bernhard Sielaff
  • Gary W Jones
  • Atin K Mandal
  • Nadinath B Nillegoda
  • Sigrun Polier
  • Douglas M Cyr
  • Hong Yu Ren
  • Johannes Buchner
  • Harald Wegele
  • Peter D Nagy
  • Xu Zheng
  • Andrew F Jarnuczak
  • Bingdong Sha
  • Jennifer L Abrams
  • Véronique Albanèse
  • Kelly C Krantz
  • Ayala Shiber
  • Christopher J Guerriero
  • Li Qiong Xu
  • Alice Yen Wen Yam
  • Daniel W Summers
  • Jungsoon Lee
  • Hong Zhang
  • Sarah Perrett
  • Andrew W Truman
  • Youtao Song
  • Yanyu Wang
  • Taras Makhnevych
  • Martin Haslbeck
  • Francis T F Tsai
  • María Moriel-Carretero
  • Nancy N Fang
  • Marie Claire Daugeron
  • Deepak Sharma
  • Christian Kohl
  • Mehdi Kabani
  • Chrisostomos Prodromou
  • Xinguo Qian
  • Rupali Prasad
  • Avrom J Caplan
  • Maria A Theodoraki
  • Michael Reidy
  • Jeannette Juretschke
  • Jozsef Stork
  • Jessica C S Brown
  • Cinzia Pagliuca
  • Meredith E K Calvert
  • A J McClellan
  • Stefan H Millson
  • Jimmy Savistchenko
  • Patrick G Needham
  • Sviatoslav N Bagriantsev
  • Ryder G Mackay
  • Kunio Nakatsukasa
  • Jocelyne Fiaux
  • Heike Rampelt
  • Harriet M Loovers
  • Stacy L Hrizo
  • Alison E Meyer
  • Rena Matsumoto
  • Céline N Martineau
  • Christine M Wright
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  • B K Baxter
  • Mitsuaki Tabuchi
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  • Elizabeth A Craig
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  • Gary Flom
  • Saulius Serva
  • Wei Zhang
  • Judith Frydman
  • R D Wegrzyn

Detail Information

Publications106 found, 100 shown here

  1. doi The Hsp70 chaperone Ssa1 is essential for catabolite induced degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase
    Jeannette Juretschke
    Institut fur Biochemie, Universitat Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany
    Biochem Biophys Res Commun 397:447-52. 2010
    ..Using tandem affinity purification and subsequent mass spectrometry we identified the Hsp70 chaperone Ssa1 as a novel interaction partner of FBPase...
  2. pmc Isolation and characterization of extragenic suppressors of mutations in the SSA hsp70 genes of Saccharomyces cerevisiae
    R J Nelson
    Department of Physiological Chemistry, University of Wisconsin Madison 53706
    Genetics 131:277-85. 1992
    Saccharomyces cerevisiae strains that contain null alleles of two hsp70 genes, SSA1 and SSA2, are temperature sensitive for growth. In this study, extragenic suppressors of ssa1 ssa2 have been isolated...
  3. pmc Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae
    M Werner-Washburne
    Mol Cell Biol 7:2568-77. 1987
    ..One subfamily, identified by sequence homology, contains four genes, SSA1, SSA2, SSA3, and SSA4 (formerly YG100, YG102, YG106, and YG107, respectively)...
  4. pmc A heat shock transcription factor with reduced activity suppresses a yeast HSP70 mutant
    J T Halladay
    Department of Biomolecular Chemistry, University of Wisconsin, Madison 53706, USA
    Mol Cell Biol 15:4890-7. 1995
    Strains carrying deletions in both the SSA1 and SSA2 HSP70 genes of Saccharomyces cerevisiae exhibit pleiotropic phenotypes, including the inability to grow at 37 degrees C or higher, reduced growth rate at permissive temperatures, ..
  5. pmc Functional interaction of cytosolic hsp70 and a DnaJ-related protein, Ydj1p, in protein translocation in vivo
    J Becker
    Department of Biomolecular Chemistry, University of Wisconsin Madison, 53706, USA
    Mol Cell Biol 16:4378-86. 1996
    ..70-kDa heat shock proteins (hsps) of Saccharomyces cerevisiae, we isolated a temperature-sensitive mutant of SSA1. The effect of a shift of mutant cells (ssa1ts ssa2 ssa3 ssa4) from the permissive temperature of 23 degrees C to ..
  6. pmc Members of the Hsp70 family of proteins in the cell wall of Saccharomyces cerevisiae
    J L Lopez-Ribot
    Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, 79430, USA
    J Bacteriol 178:4724-6. 1996
    Western blot (immunoblot) analysis of cell wall and cytosolic extracts obtained from parental and ssa1 and ssa2 single- and double-mutant strains of Saccharomyces cerevisiae showed that the heat shock protein 70 (Hsp70) products of these ..
  7. pmc In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation
    N Shulga
    Department of Biology, University of Rochester, New York 14627, USA
    J Cell Biol 135:329-39. 1996
    ..that the kinetics of NLS-directed nuclear transport in wild-type cells is stimulated by the elevated expression of SSA1, which encodes a cytoplasmic heat shock protein 70 (Hsp70)...
  8. ncbi Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins
    J R Glover
    Howard Hughes Medical Institute and Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA
    Cell 94:73-82. 1998
    ..coli. We conclude that Hsp104 has a protein remodeling activity that acts on trapped, aggregated proteins and requires specific interactions with conventional chaperones to promote refolding of the intermediates it produces...
  9. ncbi Loss of Hsp70-Hsp40 chaperone activity causes abnormal nuclear distribution and aberrant microtubule formation in M-phase of Saccharomyces cerevisiae
    M Oka
    Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    J Biol Chem 273:29727-37. 1998
    ..To elucidate the function of the yeast cytosolic hsp70 Ssa1p in vivo, we characterized a Saccharomyces cerevisiae ssa1 temperature-sensitive mutant (ssa1-134)...
  10. ncbi A nuclear export signal prevents Saccharomyces cerevisiae Hsp70 Ssb1p from stimulating nuclear localization signal-directed nuclear transport
    N Shulga
    Department of Biology, University of Rochester, Rochester, New York 14627, USA
    J Biol Chem 274:16501-7. 1999
    ..Elevated levels of SSA1 but not SSB1 suppressed the NLS-GFP nuclear localization defects of nup188-Delta cells...
  11. pmc Mutation of the ATP-binding pocket of SSA1 indicates that a functional interaction between Ssa1p and Ydj1p is required for post-translational translocation into the yeast endoplasmic reticulum
    A J McClellan
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Genetics 156:501-12. 2000
    ..To this end, mutations in the ATP-binding pocket of SSA1 were constructed and examined both in vivo and in vitro...
  12. pmc Nucleotide exchange factor for the yeast Hsp70 molecular chaperone Ssa1p
    Mehdi Kabani
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Mol Cell Biol 22:4677-89. 2002
    ..In support of this hypothesis, Fes1p was found to be associated with ribosomes...
  13. ncbi Prediction of novel Bag-1 homologs based on structure/function analysis identifies Snl1p as an Hsp70 co-chaperone in Saccharomyces cerevisiae
    Holger Sondermann
    Department of Cellular Biochemistry, Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Biol Chem 277:33220-7. 2002
    ..Thus, Snl1p is the first Bag domain protein identified in S. cerevisiae, and its interaction with Hsp70 is essential for biological activity...
  14. ncbi Sti1 is a novel activator of the Ssa proteins
    Harald Wegele
    Institut fur Organische Chemie and Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, 85747 Garching, Germany
    J Biol Chem 278:25970-6. 2003
    ..Using purified components, we reconstituted the dimeric Ssa1-Sti1 complex and the ternary Ssa1-Sti1-yHsp90 complex in vitro...
  15. pmc Exchangeable chaperone modules contribute to specification of type I and type II Hsp40 cellular function
    Chun Yang Fan
    Department of Cell and Developmental Biology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599 7090, USA
    Mol Biol Cell 15:761-73. 2004
    ..Type I and type II Hsp40s, such as yeast Ydj1 and Sis1, form chaperone pairs with cytosolic Hsp70 Ssa1 that fold proteins with different efficiencies and carry out specific cellular functions...
  16. pmc Propagation of Saccharomyces cerevisiae [PSI+] prion is impaired by factors that regulate Hsp70 substrate binding
    Gary Jones
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0851, USA
    Mol Cell Biol 24:3928-37. 2004
    ..Earlier characterization of HSP70 (SSA1) mutations suggested that [PSI(+)] propagation is impaired by alterations that enhance Ssa1p's substrate binding...
  17. pmc Hsp70 chaperones as modulators of prion life cycle: novel effects of Ssa and Ssb on the Saccharomyces cerevisiae prion [PSI+]
    Kim D Allen
    School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, 30332 0363, USA
    Genetics 169:1227-42. 2005
    PSI(+)] is a prion isoform of the yeast release factor Sup35. In some assays, the cytosolic chaperones Ssa1 and Ssb1/2 of the Hsp70 family were previously shown to exhibit "pro-[PSI(+)]" and "anti-[PSI(+)]" effects, respectively...
  18. ncbi Hsp110 cooperates with different cytosolic HSP70 systems in a pathway for de novo folding
    Alice Yen Wen Yam
    Department of Biological Sciences and BioX Program, Stanford University, Stanford, California 94305 5020, USA
    J Biol Chem 280:41252-61. 2005
    ..It, thus, appears that Hsp110 is an important regulator of Hsp70-substrate interactions. Based on our data, we propose that Hsp110 cooperates with the SSB and SSA Hsp70 subfamilies, which act sequentially during de novo folding...
  19. ncbi The yeast Hsp110 Sse1 functionally interacts with the Hsp70 chaperones Ssa and Ssb
    Lance Shaner
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030, USA
    J Biol Chem 280:41262-9. 2005
    ..Sse1 stimulated Ssa1 ATPase activity synergistically with the co-chaperone Ydj1, and stimulation required complex formation...
  20. ncbi Systems analyses reveal two chaperone networks with distinct functions in eukaryotic cells
    Véronique Albanèse
    Department of Biological Sciences and BioX Program, Stanford University, Stanford, CA 94305, USA
    Cell 124:75-88. 2006
    ..The emergence of a translation-linked chaperone network likely underlies the elaborate cotranslational folding process necessary for the evolution of larger multidomain proteins characteristic of eukaryotic cells...
  21. pmc Molecular chaperones and the assembly of the prion Sup35p, an in vitro study
    Joanna Krzewska
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Gif sur Yvette Cedex, France
    EMBO J 25:822-33. 2006
    ....
  22. pmc Chaperone network in the yeast cytosol: Hsp110 is revealed as an Hsp70 nucleotide exchange factor
    Holger Raviol
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Heidelberg, Germany
    EMBO J 25:2510-8. 2006
    ..This is the first report of a nucleotide exchange activity for the Hsp110 class of proteins, and provides a key piece in the puzzle of the cellular chaperone network...
  23. pmc Importance of the Hsp70 ATPase domain in yeast prion propagation
    Harriet M Loovers
    Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
    Genetics 175:621-30. 2007
    ..Analysis of the effects of Hsp70 mutants upon propagation of a second yeast prion [URE3] further classifies these mutants as having general or prion-specific inhibitory properties...
  24. pmc The specialized cytosolic J-protein, Jjj1, functions in 60S ribosomal subunit biogenesis
    Alison E Meyer
    Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 104:1558-63. 2007
    ....
  25. pmc The Hsp110 molecular chaperone stabilizes apolipoprotein B from endoplasmic reticulum-associated degradation (ERAD)
    Stacy L Hrizo
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Biol Chem 282:32665-75. 2007
    ..This study indicates that chaperones within distinct complexes can play unique roles during ER-associated degradation (ERAD), establishes a role for Sse1/Hsp110 in ERAD, and identifies Hsp110 as a target to lower cholesterol...
  26. pmc Dissecting the ER-associated degradation of a misfolded polytopic membrane protein
    Kunio Nakatsukasa
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Cell 132:101-12. 2008
    ..These data indicate that polytopic membrane proteins can be extracted from the ER, and define the point of action of chaperones and the requirement for Ufd2p during membrane protein quality control...
  27. doi The C-terminal extension of Saccharomyces cerevisiae Hsp104 plays a role in oligomer assembly
    Ryder G Mackay
    Department of Biochemistry, University of Toronto, 1 King s College Circle, Toronto, Ontario, Canada M5S 1A8
    Biochemistry 47:1918-27. 2008
    ..However, elimination of the whole C-terminal extension results in an Hsp104 molecule which is unable to assemble and becomes aggregation prone at high temperature, highlighting a novel structural role for this region...
  28. pmc Variant-specific [PSI+] infection is transmitted by Sup35 polymers within [PSI+] aggregates with heterogeneous protein composition
    Sviatoslav N Bagriantsev
    Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA
    Mol Biol Cell 19:2433-43. 2008
    ..show that the major components of two different variants of [PSI(+)] are interacting infectious Sup35 polymers and Ssa1/2...
  29. doi Structural basis for the cooperation of Hsp70 and Hsp110 chaperones in protein folding
    Sigrun Polier
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Cell 133:1068-79. 2008
    ....
  30. pmc Hsp110 chaperones control client fate determination in the hsp70-Hsp90 chaperone system
    Atin K Mandal
    Department of Biology, The City College of New York, New York, NY 10031, USA
    Mol Biol Cell 21:1439-48. 2010
    ..These findings support a model in which Hsp110 chaperones contribute significantly to the decision made by Hsp70 to fold or degrade a client protein...
  31. pmc A nucleus-based quality control mechanism for cytosolic proteins
    Rupali Prasad
    Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore 117604
    Mol Biol Cell 21:2117-27. 2010
    ..Instead, the Hsp70 chaperone system is needed for efficient import and degradation. These data reveal a new function of the nucleus as a compartment central to the quality control of cytosolic proteins...
  32. pmc Ubr1 and Ubr2 function in a quality control pathway for degradation of unfolded cytosolic proteins
    Nadinath B Nillegoda
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Biol Cell 21:2102-16. 2010
    ..Ubr1 and Ubr2 therefore represent E3 components of a novel quality control pathway for proteins synthesized on cytosolic ribosomes...
  33. pmc The M-domain controls Hsp104 protein remodeling activity in an Hsp70/Hsp40-dependent manner
    Bernhard Sielaff
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Mol Biol 402:30-7. 2010
    ..Our results demonstrate that the M-domain controls Hsp104 protein remodeling activities in an Hsp70/Hsp40-dependent manner, which is required to unleash Hsp104 protein disaggregating activity...
  34. doi A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry
    Virginie Redeker
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Gif sur Yvette, France
    FEBS J 277:5112-23. 2010
    ..They also reveal that the Ure2p amino-acid stretch spanning residues 327-339 plays a central role in the assembly into fibrils...
  35. pmc Single methyl group determines prion propagation and protein degradation activities of yeast heat shock protein (Hsp)-70 chaperones Ssa1p and Ssa2p
    Deepak Sharma
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:13665-70. 2011
    ..degradation pathway did not affect prion propagation, however, indicating these are two distinct processes where Ssa1/2p chaperones function differently...
  36. pmc Hul5 HECT ubiquitin ligase plays a major role in the ubiquitylation and turnover of cytosolic misfolded proteins
    Nancy N Fang
    Department of Biochemistry and Molecular Biology, Centre for High Throughput Biology, University of British Columbia, 2125 East Mall, Vancouver, British Columbia V6T 1Z4, Canada
    Nat Cell Biol 13:1344-52. 2011
    ..These findings indicate that Hul5 is involved in a cytosolic protein quality control pathway that targets misfolded proteins for degradation...
  37. pmc CDK-dependent Hsp70 Phosphorylation controls G1 cyclin abundance and cell-cycle progression
    Andrew W Truman
    Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637, USA
    Cell 151:1308-18. 2012
    In budding yeast, the essential functions of Hsp70 chaperones Ssa1-4 are regulated through expression level, isoform specificity, and cochaperone activity...
  38. pmc The Type II Hsp40 Sis1 cooperates with Hsp70 and the E3 ligase Ubr1 to promote degradation of terminally misfolded cytosolic protein
    Daniel W Summers
    Department of Cell and Developmental Biology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    PLoS ONE 8:e52099. 2013
    ..Pathways for proteasomal degradation of misfolded cytosolic proteins involve functional interplay between Type II Hsp40/Hsp70 chaperone pairs, PQC E3 ligases, and storage depots for misfolded proteins...
  39. pmc Heat shock protein (Hsp) 70 is an activator of the Hsp104 motor
    Jungsoon Lee
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 110:8513-8. 2013
    ..Consistent with a Strep-Tactin-dependent activation step, we found that full-length Hsp70 on its own could activate the Hsp104 hexamer by promoting intersubunit coordination, suggesting that Hsp70 is an activator of the Hsp104 motor...
  40. pmc The refolding activity of the yeast heat shock proteins Ssa1 and Ssa2 defines their role in protein translocation
    G L Bush
    Department of Biological Chemistry, UCLA School of Medicine 90024 1737, USA
    J Cell Biol 135:1229-37. 1996
    b>Ssa1/2p, members of one of the yeast cytosolic hsp70 subfamilies, have been implicated in the translocation of secretory proteins into the lumen of the ER...
  41. pmc Antagonistic interactions between yeast chaperones Hsp104 and Hsp70 in prion curing
    G P Newnam
    School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332 0230, USA
    Mol Cell Biol 19:1325-33. 1999
    ..Here, we show that simultaneous overexpression of Ssa1, a protein of the Hsp70 family, protects [PSI] from curing by overexpression of Hsp104...
  42. pmc Interactions between centromere complexes in Saccharomyces cerevisiae
    Vladimir S Nekrasov
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, England
    Mol Biol Cell 14:4931-46. 2003
    ..These results show an increasingly complex structure for the S. cerevisiae centromere and a probable conservation of structure between parts of the centromeres of S. cerevisiae and S. pombe...
  43. pmc Crystal structure of yeast Sis1 peptide-binding fragment and Hsp70 Ssa1 C-terminal complex
    Jingzhi Li
    Department of Cell Biology, Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, Brimingham, AL 35294 0005, USA
    Biochem J 398:353-60. 2006
    ..by determining the crystal structure of yeast Hsp40 Sis1 peptide-binding fragment complexed with the Hsp70 Ssa1 C-terminal...
  44. doi Hsp110 is a nucleotide-activated exchange factor for Hsp70
    Claes Andréasson
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZBH Alliance, Heidelberg, Germany
    J Biol Chem 283:8877-84. 2008
    ..results in formation of a stabilized conformation of Sse1 that is required for association with the yeast Hsp70 Ssa1. The interaction triggers release of bound ADP from Ssa1, but nucleotide persists bound to Sse1 in the complex...
  45. ncbi The dissociation of ATP from hsp70 of Saccharomyces cerevisiae is stimulated by both Ydj1p and peptide substrates
    T Ziegelhoffer
    Department of Biomolecular Chemistry, University of Wisconsin, Madison 53706, USA
    J Biol Chem 270:10412-9. 1995
    ..The enhanced ATP dissociation caused by both polypeptide substrates and Ydj1p may play a role in the regulation of Ssa1p chaperone activity by altering the relative abundance of ATP-and ADP-bound forms...
  46. ncbi Cytosolic Hsp70s are involved in the transport of aminopeptidase 1 from the cytoplasm into the vacuole
    C Satyanarayana
    Institut für Molekulare Zellbiologie und Biochemie, Abteilung Biochemie II, Universitat Gottingen, Heinrich Düker Weg 12, D 37073, Gottingen, Germany
    FEBS Lett 470:232-8. 2000
    ..Here we demonstrate that the constitutively expressed members of the yeast cytoplasmic Ssa subfamily, Ssa1/2p, are involved in the transport of the vacuolar hydrolase aminopeptidase 1 from the cytoplasm into the vacuole...
  47. pmc The Hsp70-Ydj1 molecular chaperone represses the activity of the heme activator protein Hap1 in the absence of heme
    T Hon
    Department of Biochemistry, NYU School of Medicine, New York, New York 10016, USA
    Mol Cell Biol 21:7923-32. 2001
    ..Our results suggest that Ssa-Ydj1 and Sro9 act together to mediate Hap1 repression in the absence of heme and that molecular chaperones promote heme regulation of Hap1 by a mechanism distinct from the mechanism of steroid signaling...
  48. pmc Proteomics analysis of the tombusvirus replicase: Hsp70 molecular chaperone is associated with the replicase and enhances viral RNA replication
    Saulius Serva
    Department of Plant Pathology, University of Kentucky, Lexington, 40546 0312, USA
    J Virol 80:2162-9. 2006
    ..The host proteins included the Ssa1/2p molecular chaperones (yeast homologues of Hsp70 proteins), Tdh2/3p (glyceraldehyde-3-phosphate dehydrogenase, an ..
  49. pmc Phosphorylation by casein kinase 2 regulates Nap1 localization and function
    Meredith E K Calvert
    Center for Cell Signaling, University of Virginia, Box 800577 HSC, Charlottesville, VA 22908, USA
    Mol Cell Biol 28:1313-25. 2008
    ..In conclusion, our data show that Nap1 phosphorylation by CK2 appears to regulate Nap1 localization and is required for normal progression through S phase...
  50. pmc Sti1 regulation of Hsp70 and Hsp90 is critical for curing of Saccharomyces cerevisiae [PSI+] prions by Hsp104
    Michael Reidy
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 30:3542-52. 2010
    ..Earlier evidence suggests that the Hsp70 mutant Ssa1-21 impairs [PSI(+)] by a related mechanism...
  51. pmc Heritable yeast prions have a highly organized three-dimensional architecture with interfiber structures
    Helen R Saibil
    Crystallography and Institute for Structural and Molecular Biology, Birkbeck College, London WC1E 7HX, United Kingdom
    Proc Natl Acad Sci U S A 109:14906-11. 2012
    ..We suggest these structures constitute a self-organizing mechanism that coordinates fiber deposition and the regulation of prion inheritance...
  52. pmc Influence of specific HSP70 domains on fibril formation of the yeast prion protein Ure2
    Li Qiong Xu
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang, Beijing 100101, People s Republic of China
    Philos Trans R Soc Lond B Biol Sci 368:20110410. 2013
    ..Constitutive overexpression of the HSP70 family member SSA1 cures cells of [URE3]...
  53. pmc Ubiquitin conjugation triggers misfolded protein sequestration into quality control foci when Hsp70 chaperone levels are limiting
    Ayala Shiber
    Department of Biological Chemistry, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Mol Biol Cell 24:2076-87. 2013
    ..We find that the heat shock protein 70 (Hsp70) chaperone pair Ssa1/Ssa2 and the Hsp40 cochaperone Sis1 are essential for degradation...
  54. pmc Hierarchical functional specificity of cytosolic heat shock protein 70 (Hsp70) nucleotide exchange factors in yeast
    Jennifer L Abrams
    From the Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030
    J Biol Chem 289:13155-67. 2014
    ....
  55. pmc Quantitative proteomics and network analysis of SSA1 and SSB1 deletion mutants reveals robustness of chaperone HSP70 network in Saccharomyces cerevisiae
    Andrew F Jarnuczak
    Faculty of Life Sciences, Michael Smith Building, Manchester, UK
    Proteomics 15:3126-39. 2015
    ..Here, we consider deletions of two major chaperone proteins, SSA1 and SSB1, from the chaperone network in Sacchromyces cerevisiae...
  56. pmc Dynamic control of Hsf1 during heat shock by a chaperone switch and phosphorylation
    Xu Zheng
    Whitehead Institute for Biomedical Research, Cambridge, United States
    elife 5:. 2016
    ..Our work reveals two uncoupled forms of regulation - an ON/OFF chaperone switch and a tunable phosphorylation gain - that allow Hsf1 to flexibly integrate signals from the proteostasis network and cell signaling pathways...
  57. pmc Clathrin coat disassembly by the yeast Hsc70/Ssa1p and auxilin/Swa2p proteins observed by single-particle burst analysis spectroscopy
    Kelly C Krantz
    From the Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544
    J Biol Chem 288:26721-30. 2013
    ....
  58. pmc The structural plasticity of Tom71 for mitochondrial precursor translocations
    Jingzhi Li
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:985-9. 2010
    ..This structural plasticity enables the precursor receptors to accommodate different precursor substrates for mitochondrial translocation...
  59. pmc The yeast Hsp70 Ssa1 is a sensor for activation of the heat shock response by thiol-reactive compounds
    Yanyu Wang
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, Houston, TX 77030, USA
    Mol Biol Cell 23:3290-8. 2012
    ..Instead, we demonstrate that the Hsp70 chaperone Ssa1, which represses Hsf1 in the absence of stress, is hypersensitive to modification by a thiol-reactive probe...
  60. pmc Hsp70 targets a cytoplasmic quality control substrate to the San1p ubiquitin ligase
    Christopher J Guerriero
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 288:18506-20. 2013
    ..These results emphasize the diverse action of molecular chaperones during CytoQC. ..
  61. pmc Control of the function of the transcription and repair factor TFIIH by the action of the cochaperone Ydj1
    María Moriel-Carretero
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla Consejo Superior de Investigaciones Científicas, 41092 Seville, Spain
    Proc Natl Acad Sci U S A 108:15300-5. 2011
    ..Our results provide evidence for a role of chaperones in NER and transcription, with implications in cancer and TFIIH-associated syndromes...
  62. pmc Hsp110 is required for spindle length control
    Taras Makhnevych
    Department of Biochemistry, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    J Cell Biol 198:623-36. 2012
    ..The role of Hsp110 Sse1 as a nucleotide exchange factor for the Hsp70 chaperones Ssa1/Ssa2 was found to be required for maintaining the proper distribution of kinesin-5 motors within the spindle, which ..
  63. ncbi Polymerization of 70-kDa heat shock protein by yeast DnaJ in ATP
    C King
    Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 270:22535-40. 1995
    ..These data suggest that, in the presence of ATP, YDJ1 may present one hsp70 to another just as under other conditions DnaJ is able to present protein substrates to DnaK...
  64. ncbi Differential regulation of Hsp70 subfamilies by the eukaryotic DnaJ homologue YDJ1
    D M Cyr
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill 27599 7260
    J Biol Chem 269:9798-804. 1994
    ..Ssa1p complexes. We propose that intramolecular communication between the polypeptide binding, ATPase and DnaJ regulatory domains on Ssa1p plays a role in the regulation of chaperone activity...
  65. ncbi Transcriptional regulation of the yeast DnaJ homologue SIS1
    T Zhong
    Cold Spring Harbor Laboratory, New York 11724, USA
    J Biol Chem 271:1349-56. 1996
    ..The elevated transcription of SIS1 in ssa1 ssa2 mutants is mediated solely through the SIS1 heat shock element...
  66. ncbi Isolation of UBP3, encoding a de-ubiquitinating enzyme, as a multicopy suppressor of a heat-shock mutant strain of S. cerevisiae
    B K Baxter
    Department of Biomolecular Chemistry, University of Wisconsin, 1300 University Avenue, Madison, WI 53706, USA
    Curr Genet 33:412-9. 1998
    Yeast strains lacking functional copies of the two genes SSA1 and SSA2, which encode cytosolic molecular chaperones, are temperature-sensitive...
  67. pmc Folding in vivo of a newly translated yeast cytosolic enzyme is mediated by the SSA class of cytosolic yeast Hsp70 proteins
    S Kim
    Department of Genetics, Yale University School of Medicine, Boyer Center, 295 Congress Avenue, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 95:12860-5. 1998
    ..refolding of OTC diluted from denaturant was assisted by crude yeast cytosol and ATP and found to be directed by SSA1/2...
  68. pmc Specific molecular chaperone interactions and an ATP-dependent conformational change are required during posttranslational protein translocation into the yeast ER
    A J McClellan
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Mol Biol Cell 9:3533-45. 1998
    ..We conclude that a conformation- and ATP-dependent interaction of BiP with the J domain of Sec63p is essential for protein translocation and that the specificity of hsc70 action is dictated by their DnaJ partners...
  69. pmc Suppression of an Hsp70 mutant phenotype in Saccharomyces cerevisiae through loss of function of the chromatin component Sin1p/Spt2p
    B K Baxter
    Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Bacteriol 180:6484-92. 1998
    ..subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded by SSA1, SSA2, SSA3, and SSA4...
  70. pmc The biochemical properties of the ATPase activity of a 70-kDa heat shock protein (Hsp70) are governed by the C-terminal domains
    P Lopez-Buesa
    Department of Biomolecular Chemistry, University of Wisconsin, 1300 University Avenue, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 95:15253-8. 1998
    ..In addition, the ATPase activity of Hsp70s, like that of Ssa1, depends on the addition of K+ whereas Ssb activity does not...
  71. ncbi Ssa1p chaperone interacts with the guanine nucleotide exchange factor of ras Cdc25p and controls the cAMP pathway in Saccharomyces cerevisiae
    M Geymonat
    Laboratoire Information Génétique et Développement, Institut de Genetique et Microbiologie, UMR CNRS Université 2225, Universite Paris Sud, Orsay, France
    Mol Microbiol 30:855-64. 1998
    ..In a strain deleted for SSA1 and SSA2, we observed a reduced cellular content of Cdc25p...
  72. pmc Mutations in VPS16 and MRT1 stabilize mRNAs by activating an inhibitor of the decapping enzyme
    S Zhang
    Department of Molecular Genetics, Robert Wood Johnson Medical School, New Jersey, USA
    Mol Cell Biol 19:7568-76. 1999
    ..Intriguingly, the interaction with Ssa1p/2p is enhanced in strains with mutations in vps16 or mrt1. We propose that Hsp70s may be involved in the regulation of mRNA decapping...
  73. ncbi Purification of protein A-tagged yeast ran reveals association with a novel karyopherin beta family member, Pdr6p
    D Lau
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    J Biol Chem 275:467-71. 2000
    ..Thus, functional tagging of yeast Ran allowed the study of its in vivo distribution and interaction with known and novel Ran-binding proteins...
  74. ncbi Purification of eukaryotic MutL homologs from Saccharomyces cerevisiae using self-affinity technology
    M C Hall
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Protein Expr Purif 21:333-42. 2001
    ..The general approach is a valid alternative for simple, rapid purification of recombinant proteins in yeast when expression in bacteria is unsuitable...
  75. ncbi The yeast hsp70 homologue Ssa is required for translation and interacts with Sis1 and Pab1 on translating ribosomes
    L E Horton
    Department of Medicine, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 276:14426-33. 2001
    ..This translational effect of Ssa appears mediated, at least in part, by its affect on the interaction of Pab1 with the translation initiation factor, eIF4G, which is dramatically reduced in the absence of functional Ssa protein...
  76. pmc Mechanism of prion loss after Hsp104 inactivation in yeast
    R D Wegrzyn
    School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332 0363, USA
    Mol Cell Biol 21:4656-69. 2001
    ..Thus, our data confirm that the primary function of Hsp104 in prion propagation is to disassemble prion aggregates and generate the small prion seeds that initiate new rounds of prion propagation (possibly assisted by Hsp70-Ssa)...
  77. pmc The nucleoporin Nup60p functions as a Gsp1p-GTP-sensitive tether for Nup2p at the nuclear pore complex
    D Denning
    Cancer Biology Program, Stanford Medical School, Stanford University, CA 94305, USA
    J Cell Biol 154:937-50. 2001
    ..The results suggest a dynamic interaction, controlled by the nucleoplasmic concentration of Gsp1p-GTP, between Nup60p and Nup2p at the NPC...
  78. pmc Direct interactions between molecular chaperones heat-shock protein (Hsp) 70 and Hsp40: yeast Hsp70 Ssa1 binds the extreme C-terminal region of yeast Hsp40 Sis1
    Xinguo Qian
    School of Life Sciences, University of Science and Technology of China, Hefei, People s Republic of China 230026
    Biochem J 361:27-34. 2002
    ..By using yeast Hsp40 Sis1 and yeast Hsp70 Ssa1 as our model proteins, we found that the Sis1 peptide-binding fragment interacts directly with the full-length Ssa1 ..
  79. pmc In vivo bipartite interaction between the Hsp40 Sis1 and Hsp70 in Saccharomyces cerevisiae
    Rebecca Aron
    Department of Biochemistry, University of Wisconsin, Madison, 53706, USA
    Genetics 169:1873-82. 2005
    ..These alterations disrupted the interaction of Sis1 with the 10-kD carboxy-terminal regulatory domain of Ssa1, indicating that Sis1 has a bipartite interaction with Ssa in vivo.
  80. ncbi Physiological effects of unassembled chaperonin Cct subunits in the yeast Saccharomyces cerevisiae
    M Anaul Kabir
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, USA
    Yeast 22:219-39. 2005
    ..Furthermore, we also suggest that the Cct subunits can act as suppressors only in certain states, such as when associated with ATP...
  81. ncbi Disassembling protein aggregates in the yeast cytosol. The cooperation of Hsp26 with Ssa1 and Hsp104
    Martin Haslbeck
    Department Chemie, Technische Universitat Munchen, D 85747 Garching, Germany
    J Biol Chem 280:23861-8. 2005
    ..Specifically, we characterized the influence of Hsp104 and Ssa1 on the disassembly of Hsp26 x substrate complexes in vitro and in vivo...
  82. pmc Purification and characterization of the three Snf1-activating kinases of Saccharomyces cerevisiae
    Karin Elbing
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Biochem J 393:797-805. 2006
    ..Finally, we showed that the Snf1 kinase domain isolated from bacteria as a GST fusion protein can be activated in vitro and shows substrate specificity in the absence of its beta and gamma subunits...
  83. pmc Effect of mutation of the tetratricopeptide repeat and asparatate-proline 2 domains of Sti1 on Hsp90 signaling and interaction in Saccharomyces cerevisiae
    Gary Flom
    Department of Microbiology, Molecular Biology and Biochemistry and the Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844 3052, USA
    Genetics 172:41-51. 2006
    ..We further examined the genetic and physical interaction of Sti1 with a mutant form of Hsp90, providing insight into the importance of the TPR2A domain of Sti1 in regulating Hsp90 function...
  84. pmc The phosphatidylinositol 4,5-biphosphate and TORC2 binding proteins Slm1 and Slm2 function in sphingolipid regulation
    Mitsuaki Tabuchi
    Department of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, La Jolla, CA 92093 0668, USA
    Mol Cell Biol 26:5861-75. 2006
    ..Together, our data suggest that Slm1 and Slm2 define a molecular link between phosphoinositide and sphingolipid signaling and thereby regulate actin cytoskeleton organization...
  85. ncbi Enhanced secretion of heterologous proteins in Pichia pastoris following overexpression of Saccharomyces cerevisiae chaperone proteins
    Wei Zhang
    Beijing Institute of Biotechnology, China
    Biotechnol Prog 22:1090-5. 2006
    ..pastoris. Therefore, the findings from the present study demonstrate the presence of a network of chaperones in vivo, which may act synergistically to increase recombinant protein yields...
  86. pmc Nucleotide-dependent interaction of Saccharomyces cerevisiae Hsp90 with the cochaperone proteins Sti1, Cpr6, and Sba1
    Jill L Johnson
    Department of Microbiology, Molecular Biology and Biochemistry and Center for Reproductive Biology, University of Idaho, Moscow, ID 83844 3052, USA
    Mol Cell Biol 27:768-76. 2007
    ....
  87. ncbi Exploring novel function of yeast Ssa1/2p by quantitative profiling proteomics using NanoESI-LC-MS/MS
    Rena Matsumoto
    International Patent Organism Depositary IPOD, National Institute of Advanced Industrial Science and Technology AIST, Central 6, 1 1 1 Higashi, Tsukuba 305 8566, Japan
    J Proteome Res 6:3465-74. 2007
    In the present study, we profiled proteins in ssa1/2 mutant and wild-type using one-dimensional gel electrophoresis coupled with liquid chromatography and mass spectrometry to reveal a total of 322 proteins...
  88. pmc Flo11p-independent control of "mat" formation by hsp70 molecular chaperones and nucleotide exchange factors in yeast
    Céline N Martineau
    Laboratoire de Microbiologie et Genetique Moleculaire, CNRS, INRA, AgroParisTech, 78850 Thiverval Grignon, France
    Genetics 177:1679-89. 2007
    ..Furthermore, complex interactions among SSA genes were observed: mat formation depended mostly on SSA1 while minor defects were observed upon loss of SSA2; additional mutations in SSA3 or SSA4 further enhanced these ..
  89. pmc Molecular chaperones and the assembly of the prion Ure2p in vitro
    Jimmy Savistchenko
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette Cedex, France
    J Biol Chem 283:15732-9. 2008
    ..Finally, the affinities of Ssa1p, Ydj1p, and Hsp104p for Ure2p are determined. Our in vitro observations bring new insight into the mechanism by which molecular chaperones influence the propagation of [URE3]...
  90. pmc Chaperone ligand-discrimination by the TPR-domain protein Tah1
    Stefan H Millson
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Biochem J 413:261-8. 2008
    ..Independent studies suggest that Tah1 interacts with Hsp90, but whether it can also interact with Hsp70/Ssa1 has not been investigated...
  91. pmc Prion-impairing mutations in Hsp70 chaperone Ssa1: effects on ATPase and chaperone activities
    Patrick G Needham
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 8, Room 407, LBG NIDDK, Bethesda, MD 20892 0851, USA
    Arch Biochem Biophys 478:167-74. 2008
    ..b>Ssa1(L483W) ATPase activity was elevated 10-fold and was least stimulated by substrates or Hsp40 co-chaperones...
  92. pmc Insights into the structural dynamics of the Hsp110-Hsp70 interaction reveal the mechanism for nucleotide exchange activity
    Claes Andréasson
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Im Neuenheimer Feld 282, D 69120 Heidelberg
    Proc Natl Acad Sci U S A 105:16519-24. 2008
    ..Here, we report the architecture of the complex between the yeast Hsp110, Sse1, and its cognate Hsp70 partner, Ssa1, as revealed by hydrogen-deuterium exchange analysis and site-specific cross-linking...
  93. pmc A key role for heat shock protein 70 in the localization and insertion of tombusvirus replication proteins to intracellular membranes
    Robert Yung Liang Wang
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    J Virol 83:3276-87. 2009
    ..To dissect the function of Hsp70 in TBSV replication, in this paper we use an Hsp70 mutant (ssa1 ssa2) yeast strain that supports a low level of TBSV replication...
  94. pmc A temperature sensitive mutant of heat shock protein 70 reveals an essential role during the early steps of tombusvirus replication
    Robert Yung Liang Wang
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546, USA
    Virology 394:28-38. 2009
    ..The constitutively expressed SSA1 and SSA2, which are resident proteins in the viral replicase, can be complemented by the heat-inducible SSA3 and/or ..
  95. pmc Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint
    Cinzia Pagliuca
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    PLoS ONE 4:e7640. 2009
    ..Moreover, comparison of orthologous proteins in yeast and humans promises to extend insight obtained from the study of simple fungal kinetochores to complex animal cell kinetochores...
  96. doi Heat, pH induced aggregation and surface hydrophobicity of S. cerevesiae Ssa1 protein
    Yusuf Tutar
    Department of Biochemistry, Faculty of Medicine, Cumhuriyet University, 58140, Sivas, Turkey
    Protein J 29:501-8. 2010
    ..For this purpose, S. cerevesiae cytosolic Hsp70 (Ssa1) biochemical properties were characterized...
  97. pmc The highly conserved eukaryotic DRG factors are required for efficient translation in a manner redundant with the putative RNA helicase Slh1
    Marie Claire Daugeron
    Equipe Labellisée La Ligue, CGM, CNRS FRE3144, 1 Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, Univ Paris Sud, Orsay F 91405, France
    Nucleic Acids Res 39:2221-33. 2011
    ....
  98. pmc Ribosome synthesis-unrelated functions of the preribosomal factor Rrp12 in cell cycle progression and the DNA damage response
    Mercedes Dosil
    Instituto de Biologia Molecular y Celular del Cancer, CSIC University of Salamanca, Campus Unamuno, E 37007 Salamanca, Spain
    Mol Cell Biol 31:2422-38. 2011
    ..I propose that the functional duality of Rrp12 may couple the control of ribosome production to the regulation of other cellular processes during cell cycle progression...
  99. doi Cooperative and independent activities of Sgt2 and Get5 in the targeting of tail-anchored proteins
    Christian Kohl
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, DKFZ ZMBH Alliance, Heidelberg, Germany
    Biol Chem 392:601-8. 2011
    ..Here, we describe the S. cerevisiae TPR protein Sgt2 as interaction partner of Ssa1 and Hsp104 and as a component of the GET pathway by interacting with Get5...
  100. pmc Reassembly and protection of small nuclear ribonucleoprotein particles by heat shock proteins in yeast cells
    A P Bracken
    Moyne Institute for Preventive Medicine, Microbiology Department, University of Dublin, Trinity College, Ireland
    RNA 5:1586-96. 1999
    ..In addition, our results show that Hsp70 plays a role in snRNP assembly under normal physiological conditions...
  101. ncbi Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae
    Ching Nen Chen
    Department of Biology, Washington University, St Louis, MO 63130, USA
    Yeast 20:545-54. 2003
    ..Our approach should be useful for identifying many of the genes that encode the metabolic pathway enzymes that have not been associated with a gene (about 275 in S. cerevisiae, by our estimate)...