SCL1

Summary

Gene Symbol: SCL1
Description: proteasome core particle subunit alpha 1
Alias: PRC2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. ncbi Structure of 20S proteasome from yeast at 2.4 A resolution
    M Groll
    Max Planck Institut fur Biochemie, Martinsreid, Germany
    Nature 386:463-71. 1997
  2. ncbi Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields active S phase cyclin-Cdk
    R Verma
    Howard Hughes Medical Institute, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Cell 8:439-48. 2001
  3. ncbi The C-terminal extension of the beta7 subunit and activator complexes stabilize nascent 20 S proteasomes and promote their maturation
    António J Marques
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, D 50674 Cologne, Germany
    J Biol Chem 282:34869-76. 2007
  4. doi Defects in Protein Folding Machinery Affect Cell Wall Integrity and Reduce Ethanol Tolerance in S. cerevisiae
    Aswathy Narayanan
    Molecular Genetics Laboratory, School of Biotechnology, National Institute of Technology Calicut, Calicut, Kerala, 673601, India
    Curr Microbiol 73:38-45. 2016
  5. doi Mistargeted mitochondrial proteins activate a proteostatic response in the cytosol
    Lidia Wrobel
    International Institute of Molecular and Cell Biology, 02 109 Warsaw, Poland
    Nature 524:485-8. 2015
  6. pmc Cytosolic Hsp60 can modulate proteasome activity in yeast
    Bella Kalderon
    From the Department of Microbiology Molecular Genetics, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 91120, Israel and
    J Biol Chem 290:3542-51. 2015
  7. pmc Conformational switching of the 26S proteasome enables substrate degradation
    Mary E Matyskiela
    Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    Nat Struct Mol Biol 20:781-8. 2013
  8. doi Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes
    Hideki Yashiroda
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Struct Mol Biol 15:228-36. 2008
  9. doi Hsm3/S5b participates in the assembly pathway of the 19S regulatory particle of the proteasome
    Benoît Le Tallec
    Laboratoire du Métabolisme de l ADN et Réponses aux Génotoxiques, SBIGeM, CEA, iBiTecS, Gif sur Yvette, F 91191, France
    Mol Cell 33:389-99. 2009
  10. pmc Proteasomes can degrade a significant proportion of cellular proteins independent of ubiquitination
    James M Baugh
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    J Mol Biol 386:814-27. 2009

Scientific Experts

  • C Enenkel
  • Aswathy Narayanan
  • Bella Kalderon
  • Lidia Wrobel
  • Mary E Matyskiela
  • Marilene Demasi
  • Benoît Le Tallec
  • Sumit Prakash
  • Olesya O Panasenko
  • James M Baugh
  • Rina Rosenzweig
  • Andrew R Kusmierczyk
  • Gustavo M Silva
  • Hideki Yashiroda
  • Damien Laporte
  • António J Marques
  • Carilee Denison
  • Dileep Pullepu
  • Praveen Kumar Reddy
  • Wasim Uddin
  • M Anaul Kabir
  • Ulrike Topf
  • Seweryn Mroczek
  • Bettina Warscheid
  • Ettel Bubis
  • Agnieszka Chacinska
  • Andrzej Dziembowski
  • Silke Oeljeklaus
  • Piotr Chrościcki
  • Piotr Bragoszewski
  • Elzbieta Januszewicz
  • Malgorzata E Sztolsztener
  • Ophry Pines
  • Gleb Kogan
  • Maciej Lirski
  • Marta Koblowska
  • Aksana Varabyova
  • Sebastian Wiese
  • Gabriel C Lander
  • Andreas Martin
  • S Jager
  • R Verma
  • Tomonao Inobe
  • Raphael Guerois
  • Ace Joseph Hatch
  • Thibault Carré
  • Andreas Matouschek
  • Anne Peyroche
  • Evgeny V Pilipenko
  • Martine A Collart
  • Fabrice P A David
  • Marie Bénédicte Barrault
  • Ekaterina G Viktorova
  • Maria Gaczynska
  • Michael H Glickman
  • Masanori Kasahara
  • Yuko Hirano
  • José A Bárcena
  • Benedicte Salin
  • Eri Sakata
  • Keiji Tanaka
  • Minoru Funakoshi
  • Atsuo Suzuki
  • Mary J Kunjappu
  • Tomie Kameyama
  • Bertrand Daignan-Fornier
  • Hidemi Hayashi
  • Koichi Kato
  • Kenji Takagi
  • D H Wolf
  • Isabelle Sagot
  • Takashi Yamane
  • Daniel C Pimenta
  • Toshihiko Kishimoto
  • Kenta Okamoto
  • Gilberto M Piassa-Filho
  • Mark Hochstrasser
  • Karen F Discola
  • Shin Ichiro Niwa
  • Tsunehiro Mizushima
  • Pawel A Osmulski
  • Eiji Kurimoto
  • Luis E S Netto
  • D Voges
  • Shigeo Murata
  • Christoph Glanemann
  • Paula C Ramos
  • R Jürgen Dohmen
  • Thomas Kodadek
  • M Groll

Detail Information

Publications24

  1. ncbi Structure of 20S proteasome from yeast at 2.4 A resolution
    M Groll
    Max Planck Institut fur Biochemie, Martinsreid, Germany
    Nature 386:463-71. 1997
    ....
  2. ncbi Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields active S phase cyclin-Cdk
    R Verma
    Howard Hughes Medical Institute, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Cell 8:439-48. 2001
    ..Activation of S-Cdk reported herein represents a complete reconstitution of the regulatory switch underlying the G1/S transition in budding yeast...
  3. ncbi The C-terminal extension of the beta7 subunit and activator complexes stabilize nascent 20 S proteasomes and promote their maturation
    António J Marques
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, D 50674 Cologne, Germany
    J Biol Chem 282:34869-76. 2007
    ..Together these data demonstrate that Blm10 and the 19 S activator have a partially redundant function in stabilizing nascent 20 S proteasomes and in promoting their activation...
  4. doi Defects in Protein Folding Machinery Affect Cell Wall Integrity and Reduce Ethanol Tolerance in S. cerevisiae
    Aswathy Narayanan
    Molecular Genetics Laboratory, School of Biotechnology, National Institute of Technology Calicut, Calicut, Kerala, 673601, India
    Curr Microbiol 73:38-45. 2016
    ..Genetic and proteomic analyses showed that the yeast genes RPS6A (ribosomal protein), SCL1 (proteasomal subunit) and TDH3 (glyceraldehyde-3-phosphate dehydrogenase) on overexpression, improved the growth of ..
  5. doi Mistargeted mitochondrial proteins activate a proteostatic response in the cytosol
    Lidia Wrobel
    International Institute of Molecular and Cell Biology, 02 109 Warsaw, Poland
    Nature 524:485-8. 2015
    ..UPRam provides a means for buffering the consequences of physiological slowdown in mitochondrial protein import and for counteracting pathologies that are caused or contributed by mitochondrial dysfunction. ..
  6. pmc Cytosolic Hsp60 can modulate proteasome activity in yeast
    Bella Kalderon
    From the Department of Microbiology Molecular Genetics, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 91120, Israel and
    J Biol Chem 290:3542-51. 2015
    ..Even mutant Hsp60 proteins, lacking chaperone activity, were still capable of proteasome inhibition. The results support the hypothesis that localization of Hsp60 to the cytosol may modulate proteasome activity according to cell need...
  7. pmc Conformational switching of the 26S proteasome enables substrate degradation
    Mary E Matyskiela
    Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    Nat Struct Mol Biol 20:781-8. 2013
    ..Notably, Rpn11 moves from an occluded position to directly above the central pore, thus facilitating substrate deubiquitination concomitant with translocation. ..
  8. doi Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes
    Hideki Yashiroda
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Struct Mol Biol 15:228-36. 2008
    ..The structure of the Dmp1-Dmp2-alpha5 complex reveals how this chaperone functions in proteasome assembly and why it dissociates from proteasome precursors before the beta-rings are assembled...
  9. doi Hsm3/S5b participates in the assembly pathway of the 19S regulatory particle of the proteasome
    Benoît Le Tallec
    Laboratoire du Métabolisme de l ADN et Réponses aux Génotoxiques, SBIGeM, CEA, iBiTecS, Gif sur Yvette, F 91191, France
    Mol Cell 33:389-99. 2009
    ..Finally, we identify the putative species-specific 19S subunit S5b as a functional homolog of the Hsm3 chaperone in mammals. These findings shed light on chaperone-assisted proteasome assembly in eukaryotes...
  10. pmc Proteasomes can degrade a significant proportion of cellular proteins independent of ubiquitination
    James M Baugh
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    J Mol Biol 386:814-27. 2009
    ..Collectively, these findings suggest a significant contribution of the ubiquitin-independent proteasome degradation pathway to the regulation of protein homeostasis in eukaryotes...
  11. pmc Ribosome association and stability of the nascent polypeptide-associated complex is dependent upon its own ubiquitination
    Olesya O Panasenko
    Swiss Institute for Bioinformatics, University of Geneva, 1211 Geneva 4, Switzerland
    Genetics 181:447-60. 2009
    ..Finally, our study demonstrated an interaction of EGD/NAC with the proteasome and revealed the importance of the Not4p E3 ligase, responsible for EGD/NAC ubiquitination, in this association...
  12. pmc Substrate selection by the proteasome during degradation of protein complexes
    Sumit Prakash
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Nat Chem Biol 5:29-36. 2009
    ..In addition, our data provide a plausible explanation for how adaptor proteins can bind to otherwise stable proteins and target them for degradation...
  13. pmc The central unit within the 19S regulatory particle of the proteasome
    Rina Rosenzweig
    Department of Biology, Technion Israel Institute of Technology, 32000 Haifa, Israel
    Nat Struct Mol Biol 15:573-80. 2008
    ..Similar pairing of units is found in unfoldases and nuclear transporters, exposing common features of these protein nanomachines...
  14. doi Role of glutaredoxin 2 and cytosolic thioredoxins in cysteinyl-based redox modification of the 20S proteasome
    Gustavo M Silva
    Instituto Butantan, Laboratorio de Bioquimica e Biofisica, Sao Paulo, Brazil, and Departamento de Genética e Biologia Evolutiva, Instituto de Biociencias, Universidade de Sao Paulo, Brazil
    FEBS J 275:2942-55. 2008
    ..These results indicate for the first time that 20S proteasome cysteinyl redox modification is a regulated mechanism coupled to enzymatic deglutathionylase activity...
  15. ncbi The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth
    E Balzi
    Unite de Biochimie Physiologique, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Gene 83:271-9. 1989
    ..The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts ..
  16. doi A multimeric assembly factor controls the formation of alternative 20S proteasomes
    Andrew R Kusmierczyk
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, Connecticut 06520 8114, USA
    Nat Struct Mol Biol 15:237-44. 2008
    ..Our data demonstrate that Pba3-Pba4 orchestrates formation of a specific type of proteasome, the first example of a trans-acting factor that controls assembly of alternative proteasomal complexes...
  17. ncbi Toward a general chemical method for rapidly mapping multi-protein complexes
    Carilee Denison
    Center for Biomedical Inventions, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas 75390 8573, USA
    J Proteome Res 3:417-25. 2004
    ....
  18. ncbi 20 S proteasome from Saccharomyces cerevisiae is responsive to redox modifications and is S-glutathionylated
    Marilene Demasi
    Departmento de Biologia, Instituto de Biociencias, Universidade de Sao Paulo, Rua do Matao, 277 São Paulo, Sao Paulo 05508 900, Brazil
    J Biol Chem 278:679-85. 2003
    ..The present results indicate that at the physiological level the yeast 20 S proteasome is regulated by its sulfhydryl content, thereby coupling intracellular redox signaling to proteasome-mediated proteolysis...
  19. pmc Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4
    S Jager
    Institut für Biochemie der Universität Stuttgart, Pfaffenwaldring 55, D 70569 Stuttgart, Germany
    EMBO J 20:4423-31. 2001
    ..Cic1 interacts in vitro and in vivo with Cdc4, suggesting a function as a new kind of substrate recruiting factor or adaptor associated with the proteasome...
  20. pmc Subcellular distribution of proteasomes implicates a major location of protein degradation in the nuclear envelope-ER network in yeast
    C Enenkel
    Institut fur Biochemie, Humboldt Universitat, Universitatsklinikum Charite, Monbijoustrasse 2, D 10117 Berlin, Germany
    EMBO J 17:6144-54. 1998
    ..A major location of proteasomal peptide cleavage activity was visualized in the NE-ER network, indicating that proteasomal degradation takes place mainly in this subcellular compartment in yeast...
  21. pmc Yeast cycloheximide-resistant crl mutants are proteasome mutants defective in protein degradation
    U M Gerlinger
    Institut fur Biochemie, Universitat Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany
    Mol Biol Cell 8:2487-99. 1997
    ..Temperature sensitivity of one of these mutants, crl3-2, had been found to be suppressed by a mutation, SCL1-1, which resided in an alpha-type subunit of the 20S proteasome...
  22. pmc Cycloheximide-resistant temperature-sensitive lethal mutations of Saccharomyces cerevisiae
    J H McCusker
    Department of Biology, Brandeis University, Waltham, Massachusetts 02254
    Genetics 119:303-15. 1988
    ..We also describe two new methods for the enrichment of auxotrophic mutations from a wild-type yeast strain...
  23. pmc Reversible cytoplasmic localization of the proteasome in quiescent yeast cells
    Damien Laporte
    Institut de Biochimie et Génétique Cellulaires, Université Victor Segalen Bordeaux II, 33077 Bordeaux, France
    J Cell Biol 181:737-45. 2008
    ..Finally, we observe conserved formation and mobilization of these PSGs in the evolutionary distant yeast Schizosaccharomyces pombe. This conservation implies a broad significance for these proteasome reserves...
  24. ncbi The 26S proteasome: a molecular machine designed for controlled proteolysis
    D Voges
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Annu Rev Biochem 68:1015-68. 1999
    ..A low-resolution structure of the 26S proteasome has been obtained by electron microscopy, but the precise arrangement of subunits in the 19S complex is unclear...