RPT2

Summary

Gene Symbol: RPT2
Description: proteasome regulatory particle base subunit RPT2
Alias: YHS4, YTA5
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Complete subunit architecture of the proteasome regulatory particle
    Gabriel C Lander
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
    Nature 482:186-91. 2012
  2. pmc The regulatory particle of the Saccharomyces cerevisiae proteasome
    M H Glickman
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 18:3149-62. 1998
  3. ncbi Rapid isolation and characterization of the yeast proteasome regulatory complex
    Y Saeki
    Department of Biochemistry, Hokkaido University, Sapporo, 060 0812, Japan
    Biochem Biophys Res Commun 273:509-15. 2000
  4. doi Hsm3/S5b participates in the assembly pathway of the 19S regulatory particle of the proteasome
    Benoît Le Tallec
    Laboratoire du Métabolisme de l ADN et Réponses aux Génotoxiques, SBIGeM, CEA, iBiTecS, Gif sur Yvette, F 91191, France
    Mol Cell 33:389-99. 2009
  5. ncbi Sem1p is a novel subunit of the 26 S proteasome from Saccharomyces cerevisiae
    Takayuki Sone
    Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    J Biol Chem 279:28807-16. 2004
  6. pmc High-resolution cryo-EM structure of the proteasome in complex with ADP-AlFx
    Zhanyu Ding
    National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences University of Chinese Academy of Sciences, 333 Haike Road, Shanghai 201203, China
    Cell Res . 2017
  7. pmc 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay
    Kotaro Fujii
    Institute for Virus Research, Kyoto University, Kyoto, Japan
    EMBO J 31:2579-89. 2012
  8. doi N-myristoylation of the Rpt2 subunit regulates intracellular localization of the yeast 26S proteasome
    Ayuko Kimura
    Advanced Medical Research Center, Yokohama City University, Fukuura 3 9, Kanazawa, Yokohama 236 0004, Japan
    Biochemistry 51:8856-66. 2012
  9. ncbi Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity
    Y Saeki
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan
    Methods Enzymol 399:215-27. 2005
  10. pmc Base-CP proteasome can serve as a platform for stepwise lid formation
    Zanlin Yu
    Department of Biology, Technion Israel Institute of Technology, 32000 Haifa, Israel
    Biosci Rep 35:. 2015

Detail Information

Publications28

  1. pmc Complete subunit architecture of the proteasome regulatory particle
    Gabriel C Lander
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
    Nature 482:186-91. 2012
    ..We provide a structural basis for the ability of the proteasome to degrade a diverse set of substrates and thus regulate vital cellular processes...
  2. pmc The regulatory particle of the Saccharomyces cerevisiae proteasome
    M H Glickman
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 18:3149-62. 1998
    ..Overall, regulatory particles from yeasts and mammals are remarkably similar, suggesting that the specific mechanistic features of the proteasome have been closely conserved over the course of evolution...
  3. ncbi Rapid isolation and characterization of the yeast proteasome regulatory complex
    Y Saeki
    Department of Biochemistry, Hokkaido University, Sapporo, 060 0812, Japan
    Biochem Biophys Res Commun 273:509-15. 2000
    ..In contrast with the previously reported result showing that Rpn10, a multiubiquitin chain binding subunit, is a component of the base complex, we present evidence that the lid complex isolated from wild-type yeast contains Rpn10...
  4. doi Hsm3/S5b participates in the assembly pathway of the 19S regulatory particle of the proteasome
    Benoît Le Tallec
    Laboratoire du Métabolisme de l ADN et Réponses aux Génotoxiques, SBIGeM, CEA, iBiTecS, Gif sur Yvette, F 91191, France
    Mol Cell 33:389-99. 2009
    ..Finally, we identify the putative species-specific 19S subunit S5b as a functional homolog of the Hsm3 chaperone in mammals. These findings shed light on chaperone-assisted proteasome assembly in eukaryotes...
  5. ncbi Sem1p is a novel subunit of the 26 S proteasome from Saccharomyces cerevisiae
    Takayuki Sone
    Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    J Biol Chem 279:28807-16. 2004
    ..The results suggest that Sem1, possibly hDSS1, is a novel subunit of the 26 S proteasome and plays a role in ubiquitin-dependent proteolysis...
  6. pmc High-resolution cryo-EM structure of the proteasome in complex with ADP-AlFx
    Zhanyu Ding
    National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences University of Chinese Academy of Sciences, 333 Haike Road, Shanghai 201203, China
    Cell Res . 2017
    ..The remaining two subunits, Rpt2 and Rpt6, with empty or only partially occupied nucleotide pocket exhibit pronounced conformational changes in the ..
  7. pmc 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay
    Kotaro Fujii
    Institute for Virus Research, Kyoto University, Kyoto, Japan
    EMBO J 31:2579-89. 2012
    ....
  8. doi N-myristoylation of the Rpt2 subunit regulates intracellular localization of the yeast 26S proteasome
    Ayuko Kimura
    Advanced Medical Research Center, Yokohama City University, Fukuura 3 9, Kanazawa, Yokohama 236 0004, Japan
    Biochemistry 51:8856-66. 2012
    ..Here, we report that the N-myristoylation of the Rpt2 subunit controls the intracellular localization of the 26S proteasome...
  9. ncbi Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity
    Y Saeki
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan
    Methods Enzymol 399:215-27. 2005
    ..In this communication, we describe that Sic1 was successfully ubiquitinated by the PY motif-insertion method and demonstrate that Sic1 thus ubiquitinated was degraded by the purified yeast 26S proteasome...
  10. pmc Base-CP proteasome can serve as a platform for stepwise lid formation
    Zanlin Yu
    Department of Biology, Technion Israel Institute of Technology, 32000 Haifa, Israel
    Biosci Rep 35:. 2015
    ....
  11. pmc Ubp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome
    Charlene Bashore
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA
    Nat Struct Mol Biol 22:712-9. 2015
    ..Ubp6 may thus act as a ubiquitin-dependent 'timer' to coordinate individual processing steps at the proteasome and modulate substrate degradation. ..
  12. ncbi The axial channel of the proteasome core particle is gated by the Rpt2 ATPase and controls both substrate entry and product release
    A Kohler
    Department of Cell Biology, Harvard Medical School, 02115, Boston, MA, USA
    Mol Cell 7:1143-52. 2001
    ..Using yeast mutants, we show that channel opening is mediated by the ATPase domain of Rpt2, one of six ATPases in the RP...
  13. doi N-Myristoylation of the Rpt2 subunit of the yeast 26S proteasome is implicated in the subcellular compartment-specific protein quality control system
    Ayuko Kimura
    Advanced Medical Research Center, Yokohama City University, Fukuura 3 9, Kanazawa, Yokohama 236 0004, Japan Graduate School of Medical Life Science, Yokohama City University, Fukuura 3 9, Kanazawa, Yokohama 236 0004, Japan
    J Proteomics 130:33-41. 2016
    ..the yeast proteasome is maintained by the importin-mediated transport, N-myristoylation of the proteasome subunit Rpt2 was indicated to cause dynamic nucleo-cytoplasmic localization of proteasomes...
  14. pmc Activation of the Yeast UBI4 Polyubiquitin Gene by Zap1 Transcription Factor via an Intragenic Promoter Is Critical for Zinc-deficient Growth
    Colin W MacDiarmid
    From the Department of Nutritional Sciences, University of Wisconsin, Madison, Wisconsin 53706 and
    J Biol Chem 291:18880-96. 2016
    ..In a screen for ubi4Δ suppressors, a hypomorphic allele of the RPT2 proteasome regulatory subunit gene (rpt2(E301K)) suppressed the ubi4Δ growth defect...
  15. doi Separation of a functional deubiquitylating module from the SAGA complex by the proteasome regulatory particle
    Sungsu Lim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    Nat Commun 4:2641. 2013
    ..We show that the localization of the Sgf73-DUBm on chromatin is perturbed in rpt2-1, a strain of Saccharomyces cerevisiae that is specifically defective in the Rpt2p-Sgf73p interaction...
  16. pmc Conformational switching of the 26S proteasome enables substrate degradation
    Mary E Matyskiela
    Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    Nat Struct Mol Biol 20:781-8. 2013
    ..Notably, Rpn11 moves from an occluded position to directly above the central pore, thus facilitating substrate deubiquitination concomitant with translocation. ..
  17. pmc Structural basis for specific recognition of Rpt1p, an ATPase subunit of 26 S proteasome, by proteasome-dedicated chaperone Hsm3p
    Kenji Takagi
    Picobiology Institute, Department of Life Science, Graduate School of Life Science, University of Hyogo, 3 2 1, Kouto, Kamigori cho, Ako gun, Hyogo, 678 1297, Japan
    J Biol Chem 287:12172-82. 2012
    ..chaperone, transiently binds to the C-terminal domain of the Rpt1 subunit and forms a tetrameric complex, Hsm3-Rpt1-Rpt2-Rpn1, during maturation of the ATPase ring of 19 S RP...
  18. pmc Dual functions of the Hsm3 protein in chaperoning and scaffolding regulatory particle subunits during the proteasome assembly
    Marie Bénédicte Barrault
    Commissariat a l Energie Atomique CEA, Institut de Biologie et Technologies de Saclay iBiTec S, Service de Biologie Intégrative et Génétique Moléculaire, Laboratoire du Métabolisme des Acides Nucléiques et Réponses aux Génotoxiques, F 91191 Gif sur Yvette, France
    Proc Natl Acad Sci U S A 109:E1001-10. 2012
    ..is important for normal growth and directly targets the carboxyl-terminal (C-terminal) domain of Rpt1 of the Rpt1-Rpt2-Rpn1 assembly intermediate...
  19. pmc Hexameric assembly of the proteasomal ATPases is templated through their C termini
    SoYeon Park
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 459:866-70. 2009
    ..Our studies show that assembly of the proteasome base is a rapid yet highly orchestrated process...
  20. doi Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle
    Yasushi Saeki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    Cell 137:900-13. 2009
    ..Our results indicate that the RP assembly is a highly organized and elaborate process orchestrated by multiple proteasome-dedicated chaperones...
  21. ncbi Cloning and expression of a yeast gene encoding a protein with ATPase activity and high identity to the subunit 4 of the human 26 S protease
    H A Lucero
    Department of Physiology, Boston University School of Medicine, Massachusetts 02118, USA
    J Biol Chem 270:9178-84. 1995
    ..N-Ethylmaleimide inhibits the ATPase activity of both forms of Yhs4p. We propose that Yhs4p ATPase may play an essential role in the regulatory function of the proteolytic activity of the yeast 26 S protease...
  22. pmc Ribosome association and stability of the nascent polypeptide-associated complex is dependent upon its own ubiquitination
    Olesya O Panasenko
    Swiss Institute for Bioinformatics, University of Geneva, 1211 Geneva 4, Switzerland
    Genetics 181:447-60. 2009
    ..Finally, our study demonstrated an interaction of EGD/NAC with the proteasome and revealed the importance of the Not4p E3 ligase, responsible for EGD/NAC ubiquitination, in this association...
  23. ncbi The bipartite nuclear localization sequence of Rpn2 is required for nuclear import of proteasomal base complexes via karyopherin alphabeta and proteasome functions
    Petra Wendler
    Institut für Biochemie CCM, Charite, Universitatsmedizin Berlin, Monbijoustrasse 2, D 10117 Berlin, Germany
    J Biol Chem 279:37751-62. 2004
    ..Potential classic nuclear localization sequences (NLSs) of base subunits were analyzed. Rpn2 and Rpt2, a non-ATPase subunit and an ATPase subunit of the base complex, harbor functional NLSs...
  24. ncbi Recombinant ATPases of the yeast 26S proteasome activate protein degradation by the 20S proteasome
    Junko Takeuchi
    Proteolysis and Protein Turnover Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, 2 17 2 1 Tsukisamu Higashi, Toyohira ku, Sapporo 062 8517, Japan
    FEBS Lett 565:39-42. 2004
    ..Our finding, production of a functional subunit of the 19S regulatory particle in bacteria, is a simpler and technically advanced system to functionally characterize individual subunits...
  25. ncbi Recruitment of a 19S proteasome subcomplex to an activated promoter
    Fernando Gonzalez
    Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 8573, USA
    Science 296:548-50. 2002
    ..These data indicate that in vivo, the base of the 19S complex functions independently of the larger complex and plays a direct, nonproteolytic role in RNA polymerase II transcription...
  26. pmc Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome
    D M Rubin
    Department of Cell Biology, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA
    EMBO J 17:4909-19. 1998
    ..Proteasomes purified from an rpt2 mutant showed a dramatic inhibition of peptidase activity, suggesting a defect in gating of the proteasome channel...
  27. ncbi Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex
    R Schnall
    Institut fur Physiologische Chemie, Universitat Munchen, Germany
    Yeast 10:1141-55. 1994
    ..YTA1, YTA2, YTA3 and YTA5 exhibit significant similarity to proteins involved in human immunodeficiency virus Tat-mediated gene expression ..
  28. ncbi Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle
    D Finley
    Trends Biochem Sci 23:244-5. 1998