RPN8

Summary

Gene Symbol: RPN8
Description: proteasome regulatory particle lid subunit RPN8
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. ncbi Sem1p is a novel subunit of the 26 S proteasome from Saccharomyces cerevisiae
    Takayuki Sone
    Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    J Biol Chem 279:28807-16. 2004
  2. pmc Complete subunit architecture of the proteasome regulatory particle
    Gabriel C Lander
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
    Nature 482:186-91. 2012
  3. ncbi Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae
    Erika Isono
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    J Biol Chem 279:27168-76. 2004
  4. ncbi N-Terminal modifications of the 19S regulatory particle subunits of the yeast proteasome
    Yayoi Kimura
    Kihara Institute for Biological Research Graduate School of Integrated Science, Yokohama City University, Maioka 641 12, Totsuka, Yokohama, Japan
    Arch Biochem Biophys 409:341-8. 2003
  5. pmc The regulatory particle of the Saccharomyces cerevisiae proteasome
    M H Glickman
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 18:3149-62. 1998
  6. pmc Atomic structure of the 26S proteasome lid reveals the mechanism of deubiquitinase inhibition
    Corey M Dambacher
    Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, United States
    elife 5:e13027. 2016
  7. pmc The Proteasome Subunit Rpn8 Interacts with the Small Nucleolar RNA Protein (snoRNP) Assembly Protein Pih1 and Mediates Its Ubiquitin-independent Degradation in Saccharomyces cerevisiae
    Alexandr Paci
    From the Department of Biological Sciences, University of Toronto, Toronto, Ontario M1C 1A4, Canada
    J Biol Chem 291:11761-75. 2016
  8. pmc A Single α Helix Drives Extensive Remodeling of the Proteasome Lid and Completion of Regulatory Particle Assembly
    Robert J Tomko
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA Electronic address
    Cell 163:432-44. 2015
  9. pmc Solution Structure of the Cuz1 AN1 Zinc Finger Domain: An Exposed LDFLP Motif Defines a Subfamily of AN1 Proteins
    Zhen Yu J Sun
    Department of Biological Chemistry and Molecular Physiology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 11:e0163660. 2016
  10. pmc High-resolution cryo-EM structure of the proteasome in complex with ADP-AlFx
    Zhanyu Ding
    National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences University of Chinese Academy of Sciences, 333 Haike Road, Shanghai 201203, China
    Cell Res . 2017

Scientific Experts

  • Robert J Tomko
  • M Hochstrasser
  • Andreas Martin
  • Gabriel C Lander
  • Michael H Glickman
  • Yasushi Saeki
  • Zanlin Yu
  • Wissam Mansour
  • Evan J Worden
  • Mary E Matyskiela
  • Eric Estrin
  • Zhanyu Ding
  • Alexandr Paci
  • Thomas Taverner
  • Corey M Dambacher
  • Zhen Yu J Sun
  • Michal Sharon
  • Mark A Nakasone
  • Oded Kleifeld
  • David Fushman
  • Lee Zeev Peters
  • Noa Reis
  • Ganesh Ramnath Pathare
  • Akio Toh-E
  • Grégory Bonfils
  • Fuqiang Geng
  • Hideyoshi Yokosawa
  • Abhishek Chandra
  • Elah Pick
  • Minoru Funakoshi
  • Damien Laporte
  • Li Chen
  • Kiran Madura
  • Carol V Robinson
  • Irina Brandina
  • Show Mei Chuang
  • Y Saeki
  • Erika Isono
  • Teresa Rinaldi
  • Takayuki Sone
  • Rongguang Zhang
  • Liangliang Kong
  • Zhenglin Fu
  • Yao Cong
  • Junrui Li
  • Na Li
  • Yifan Wang
  • Jinhuan Chen
  • Yanxing Wang
  • Cong Xu
  • Yayoi Kimura
  • John Hanna
  • Rongmin Zhao
  • Mark A Herzik
  • Gerhard Wagner
  • Peter X H Liu
  • Haribabu Arthanari
  • Martin G Allan
  • Lingjie Zhang
  • Meera K Bhanu
  • Carlos A Castaneda
  • Emma K Dixon
  • Galit David-Kadoch
  • Nurit Livnat-Levanon
  • Shay Ben-Aroya
  • Ofri Karmon
  • Thomas Sommer
  • Tzenlin Yu
  • Rotem Hazan
  • Maximilian von Delbrück
  • Daria Krutauz
  • Friedrich Förster
  • Istvan Nagy
  • Chris Padovani
  • Wolfgang Baumeister
  • Els Pardon
  • Daniel J Anderson
  • Andreas Bracher
  • Jan Steyaert
  • Paweł Sledź
  • D Finley
  • Han Jie Zhou
  • José Ramón Lopéz-Blanco
  • A Toh-e
  • Pablo Chacon
  • Charlene Bashore
  • Eva Nogales
  • William P Tansey
  • Malika Jaquenoud
  • Christian Ungermann

Detail Information

Publications35

  1. ncbi Sem1p is a novel subunit of the 26 S proteasome from Saccharomyces cerevisiae
    Takayuki Sone
    Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    J Biol Chem 279:28807-16. 2004
    ..The results suggest that Sem1, possibly hDSS1, is a novel subunit of the 26 S proteasome and plays a role in ubiquitin-dependent proteolysis...
  2. pmc Complete subunit architecture of the proteasome regulatory particle
    Gabriel C Lander
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
    Nature 482:186-91. 2012
    ..We provide a structural basis for the ability of the proteasome to degrade a diverse set of substrates and thus regulate vital cellular processes...
  3. ncbi Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae
    Erika Isono
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    J Biol Chem 279:27168-76. 2004
    ..From these results, we concluded that Rpn7 is required for the integrity of the 26 S complex by establishing a correct lid structure...
  4. ncbi N-Terminal modifications of the 19S regulatory particle subunits of the yeast proteasome
    Yayoi Kimura
    Kihara Institute for Biological Research Graduate School of Integrated Science, Yokohama City University, Maioka 641 12, Totsuka, Yokohama, Japan
    Arch Biochem Biophys 409:341-8. 2003
    ..nat1, nat3, and mak3 deletion mutants, we found that 8 subunits, Rpt4, Rpt5, Rpt6, Rpn2, Rpn3, Rpn5, Rpn6, and Rpn8, were NatA substrates, and that 2 subunits, Rpt3 and Rpn11, were NatB substrates...
  5. pmc The regulatory particle of the Saccharomyces cerevisiae proteasome
    M H Glickman
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 18:3149-62. 1998
    ..Overall, regulatory particles from yeasts and mammals are remarkably similar, suggesting that the specific mechanistic features of the proteasome have been closely conserved over the course of evolution...
  6. pmc Atomic structure of the 26S proteasome lid reveals the mechanism of deubiquitinase inhibition
    Corey M Dambacher
    Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, United States
    elife 5:e13027. 2016
    ....
  7. pmc The Proteasome Subunit Rpn8 Interacts with the Small Nucleolar RNA Protein (snoRNP) Assembly Protein Pih1 and Mediates Its Ubiquitin-independent Degradation in Saccharomyces cerevisiae
    Alexandr Paci
    From the Department of Biological Sciences, University of Toronto, Toronto, Ontario M1C 1A4, Canada
    J Biol Chem 291:11761-75. 2016
    ..we investigated Pih1 interactors and identified a specific interaction between Pih1 and the proteasome subunit Rpn8 in yeast Saccharomyces cerevisiae when HSP90 co-chaperone Tah1 is depleted...
  8. pmc A Single α Helix Drives Extensive Remodeling of the Proteasome Lid and Completion of Regulatory Particle Assembly
    Robert J Tomko
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA Electronic address
    Cell 163:432-44. 2015
    ..Such assembly-coupled conformational switching is reminiscent of viral particle maturation and may represent a commonly used mechanism to enforce hierarchical assembly in multisubunit complexes...
  9. pmc Solution Structure of the Cuz1 AN1 Zinc Finger Domain: An Exposed LDFLP Motif Defines a Subfamily of AN1 Proteins
    Zhen Yu J Sun
    Department of Biological Chemistry and Molecular Physiology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 11:e0163660. 2016
    ..These results provide the first structural characterization of the AN1 zinc finger domain, and suggest that the LDFLP motif may define a sub-family of evolutionarily conserved AN1 zinc finger proteins...
  10. pmc High-resolution cryo-EM structure of the proteasome in complex with ADP-AlFx
    Zhanyu Ding
    National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences University of Chinese Academy of Sciences, 333 Haike Road, Shanghai 201203, China
    Cell Res . 2017
    ..Cell Research advance online publication 20 January 2017; doi:10.1038/cr.2017.12...
  11. pmc Base-CP proteasome can serve as a platform for stepwise lid formation
    Zanlin Yu
    Department of Biology, Technion Israel Institute of Technology, 32000 Haifa, Israel
    Biosci Rep 35:. 2015
    ..Even within the lid, subunits have been demarcated into two modules: module 1 (Rpn5, Rpn6, Rpn8, Rpn9 and Rpn11), which interacts with both CP and base sub-complexes and module 2 (Rpn3, Rpn7, Rpn12 and Rpn15) ..
  12. pmc The protein quality control machinery regulates its misassembled proteasome subunits
    Lee Zeev Peters
    Faculty of life sciences Bar Ilan University, Ramat Gan, Israel
    PLoS Genet 11:e1005178. 2015
    ..Thus, we show that proteasome homeostasis is controlled through probing the level of proteasome assembly, and the interplay between UPS mediated degradation or their sorting into distinct cellular compartments. ..
  13. pmc Disassembly of Lys11 and mixed linkage polyubiquitin conjugates provides insights into function of proteasomal deubiquitinases Rpn11 and Ubp6
    Wissam Mansour
    From the Department of Biology, Technion Israel Institute of Technology, 32000 Haifa, Israel
    J Biol Chem 290:4688-704. 2015
    ..The reduced ability to disassemble homogeneous Lys(48)-linked chains longer than 4 Ub units may prolong residency time on the proteasome. ..
  14. pmc Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11
    Ganesh Ramnath Pathare
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Proc Natl Acad Sci U S A 111:2984-9. 2014
    ..Here we describe three crystal structures of the heterodimer of the Mpr1-Pad1-N-terminal domains of Rpn8 and Rpn11, crystallized as a fusion protein in complex with a nanobody...
  15. doi Structure of the Rpn11-Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation
    Evan J Worden
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA
    Nat Struct Mol Biol 21:220-7. 2014
    ..crystal structures of Zn(2+)-free and Zn(2+)-bound Saccharomyces cerevisiae Rpn11 in an MPN-domain heterodimer with Rpn8. The Rpn11-Rpn8 interaction occurs via two distinct interfaces that may be conserved in related MPN-domain ..
  16. pmc The intrinsically disordered Sem1 protein functions as a molecular tether during proteasome lid biogenesis
    Robert J Tomko
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520 8114, USA
    Mol Cell 53:433-43. 2014
    ..Thus, although Sem1 is a stoichiometric component of the mature proteasome, it has a distinct, chaperone-like function specific to early stages of proteasome assembly...
  17. doi Formation of an intricate helical bundle dictates the assembly of the 26S proteasome lid
    Eric Estrin
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Structure 21:1624-35. 2013
    ..Finally, we predict that the assembly of the COP9 signalosome depends on a similar helical bundle...
  18. pmc Proteasome-mediated degradation of cotranslationally damaged proteins involves translation elongation factor 1A
    Show Mei Chuang
    Department of Biochemistry, Robert Wood Johnson Medical School, 683 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Cell Biol 25:403-13. 2005
    ..Our findings provide a mechanistic foundation for defining how cellular proteins are degraded cotranslationally...
  19. ncbi Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity
    Y Saeki
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan
    Methods Enzymol 399:215-27. 2005
    ..In this communication, we describe that Sic1 was successfully ubiquitinated by the PY motif-insertion method and demonstrate that Sic1 thus ubiquitinated was degraded by the purified yeast 26S proteasome...
  20. pmc Incorporation of the Rpn12 subunit couples completion of proteasome regulatory particle lid assembly to lid-base joining
    Robert J Tomko
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Mol Cell 44:907-17. 2011
    ..Rpn12 incorporation thus links proper lid assembly to subsequent assembly steps...
  21. ncbi Ubiquitin-dependent protein degradation
    M Hochstrasser
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA
    Annu Rev Genet 30:405-39. 1996
    ..This diversity underlies both the high substrate specificity of the ubiquitin system and the variety of regulatory mechanisms that it serves...
  22. pmc Conformational switching of the 26S proteasome enables substrate degradation
    Mary E Matyskiela
    Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    Nat Struct Mol Biol 20:781-8. 2013
    ..Notably, Rpn11 moves from an occluded position to directly above the central pore, thus facilitating substrate deubiquitination concomitant with translocation. ..
  23. pmc Similar temporal and spatial recruitment of native 19S and 20S proteasome subunits to transcriptionally active chromatin
    Fuqiang Geng
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, 465 21st Avenue South, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 109:6060-5. 2012
    ..We find that proteasome subunits Rpt1, Rpt4, Rpn8, Rpn12, Pre6, and Pre10 are recruited to GAL10 rapidly upon galactose induction...
  24. doi Leucyl-tRNA synthetase controls TORC1 via the EGO complex
    Grégory Bonfils
    Department of Biology, Division of Biochemistry, University of Fribourg, CH 1700 Fribourg, Switzerland
    Mol Cell 46:105-10. 2012
    ..Thus, the EGOC-TORC1 signaling module samples, via the LeuRS-intrinsic editing domain, the fidelity of tRNA(Leu) aminoacylation as a proxy for leucine availability...
  25. pmc Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base
    Minoru Funakoshi
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520 8114, USA
    Cell 137:887-99. 2009
    ..Our results demonstrate that proteasomal RP biogenesis requires multiple, functionally overlapping chaperones and suggest a model in which subunits form specific subcomplexes that then assemble into the base...
  26. pmc Reversible cytoplasmic localization of the proteasome in quiescent yeast cells
    Damien Laporte
    Institut de Biochimie et Génétique Cellulaires, Université Victor Segalen Bordeaux II, 33077 Bordeaux, France
    J Cell Biol 181:737-45. 2008
    ..Finally, we observe conserved formation and mobilization of these PSGs in the evolutionary distant yeast Schizosaccharomyces pombe. This conservation implies a broad significance for these proteasome reserves...
  27. doi Subunit architecture of intact protein complexes from mass spectrometry and homology modeling
    Thomas Taverner
    Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge CB2 1EW, UK
    Acc Chem Res 41:617-27. 2008
    ..Overall therefore this mass spectrometry and homology modeling approach has given significant insight into the structure of two previously intractable protein complexes and as such has broad application in structural biology...
  28. ncbi tRNA import into yeast mitochondria is regulated by the ubiquitin-proteasome system
    Irina Brandina
    Unité Mixte de Recherche 7156 GMGM, Department of Molecular and Cellular Genetics, Centre National de la Recherche Scientifique ULP, 21 rue René Descartes, Strasbourg 67084, France
    FEBS Lett 581:4248-54. 2007
    ..This result suggests a functional link between UPS and tRNA mitochondrial import in yeast and indicates on the existence of negative and positive import regulators...
  29. pmc Structural organization of the 19S proteasome lid: insights from MS of intact complexes
    Michal Sharon
    Department of Chemistry, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 4:e267. 2006
    ..More generally, the results highlight the potential of mass spectrometry to add crucial insight into the structural organization of an endogenous, wild-type complex...
  30. doi Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle
    Yasushi Saeki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    Cell 137:900-13. 2009
    ..Our results indicate that the RP assembly is a highly organized and elaborate process orchestrated by multiple proteasome-dedicated chaperones...
  31. pmc Participation of the proteasomal lid subunit Rpn11 in mitochondrial morphology and function is mapped to a distinct C-terminal domain
    Teresa Rinaldi
    Pasteur Institute Cenci Bolognetti Foundation and the Department of Cell and Developmental Biology, University of Rome I, 00185 Rome, Italy
    Biochem J 381:275-85. 2004
    ..We find that overexpression of WT (wild-type) RPN8, encoding a paralogous subunit that does not contain the catalytic MPN+ motif, corrects proteasome conformations ..
  32. pmc Evidence for an interaction between ubiquitin-conjugating enzymes and the 26S proteasome
    P Tongaonkar
    Department of Biochemistry, Robert Wood Johnson Medical School UMDNJ, Piscataway, NJ 08854, USA
    Mol Cell Biol 20:4691-8. 2000
    ..Purified proteasomes can ligate ubiquitin to a test substrate without the addition of exogenous E2 protein, suggesting that the ubiquitylation of some proteolytic substrates might be directly coupled to degradation by the proteasome...
  33. pmc Recovery from DNA replicational stress is the essential function of the S-phase checkpoint pathway
    B A Desany
    Verna and Marrs McLean Department of Biochemistry, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030 USA
    Genes Dev 12:2956-70. 1998
    ..We propose that this checkpoint pathway plays an important role in the maintenance of DNA synthetic capabilities when DNA replication is stressed...
  34. ncbi Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle
    D Finley
    Trends Biochem Sci 23:244-5. 1998
  35. pmc Synthetic lethality of rpn11-1 rpn10Δ is linked to altered proteasome assembly and activity
    Abhishek Chandra
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Curr Genet 56:543-57. 2010
    ..Based on these findings, we propose that the lethality of rpn11-1 rpn10Δ results primarily from altered proteasome integrity. It is conceivable that Rpn10/Rpn11 interaction couples proteasome assembly to substrate binding...