RLF2

Summary

Gene Symbol: RLF2
Description: Rlf2p
Alias: CAC1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae
    S H Meijsing
    Otto Warburg Laboratorium, Max Planck Institut fur Molekulare Genetik, D 14195 Berlin, Germany
    Genes Dev 15:3169-82. 2001
  2. ncbi A role for cell-cycle-regulated histone H3 lysine 56 acetylation in the DNA damage response
    Hiroshi Masumoto
    Chromosome Dynamics Laboratory, Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 436:294-8. 2005
  3. pmc Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing
    Shengbing Huang
    Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Proc Natl Acad Sci U S A 102:13410-5. 2005
  4. pmc Checkpoint functions are required for normal S-phase progression in Saccharomyces cerevisiae RCAF- and CAF-I-defective mutants
    Ellen S Kats
    Ludwig Institute for Cancer Research, Department of Medicine, University of California at San Diego School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0669, USA
    Proc Natl Acad Sci U S A 103:3710-5. 2006
  5. ncbi A Rad53 kinase-dependent surveillance mechanism that regulates histone protein levels in S. cerevisiae
    Akash Gunjan
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Cell 115:537-49. 2003
  6. pmc Structural basis for the histone chaperone activity of Asf1
    Christine M English
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, CO 80045, USA
    Cell 127:495-508. 2006
  7. pmc Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56
    Robert Driscoll
    Wellcome Trust and Cancer Research U K Gurdon Institute and the Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Science 315:649-52. 2007
  8. pmc Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly
    Qing Li
    Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Cell 134:244-55. 2008
  9. pmc Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete
    Jung Ae Kim
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 106:1151-6. 2009
  10. pmc Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing
    Denise C Krawitz
    Lawrence Berkeley National Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Mol Cell Biol 22:614-25. 2002

Research Grants

  1. RECOMBINATION MECHANISMS IN YEAST CELL DIFFERENTIATION
    James Haber; Fiscal Year: 2006
  2. Mechanism of in-vitro aging
    LYSLE LEWIS; Fiscal Year: 2007

Scientific Experts

  • Troy A A Harkness
  • P D Kaufman
  • LYSLE LEWIS
  • KRISTINA HILDEGARD SCHMIDT
  • James Haber
  • Zhiguo Zhang
  • Rebecca J Burgess
  • Hui Zhou
  • Qing Li
  • Wallace H Liu
  • Judith A Sharp
  • Jessica K Tyler
  • Junhong Han
  • Jiayi Yang
  • Brandon Wyse
  • Kimiko Saka
  • Sarah C Roemer
  • Alain Verreault
  • Akash Gunjan
  • Ellen S Kats
  • Betty P K Poon
  • Dan Su
  • Rachel M Zunder
  • Yao Yu
  • Jessica Lopes da Rosa
  • Marta Clemente-Ruiz
  • Nidhi Vishnoi
  • Marlies P Rossmann
  • Kyungjae Myung
  • Jason Feser
  • Yiwei Liu
  • Mair E A Churchill
  • Jung Ae Kim
  • Rakesh Kumar Singh
  • Adriana Miele
  • Judith A Erkmann
  • Denise C Krawitz
  • Ana Poveda
  • Joshua J Carson
  • Lindsey J Bostelman
  • Kazuya Matsuda
  • Zachary L Pratt
  • Robert Driscoll
  • Christine M English
  • Andrew P VanDemark
  • Susan R Zabaronick
  • Shengbing Huang
  • Hiroshi Masumoto
  • Richard D Kolodner
  • Soma Banerjee
  • Tetsushi Iida
  • Lynn Glowczewski
  • Jianxun Feng
  • Yeyun Zhou
  • Travis Nemkov
  • Sanna Abbasi
  • Akihiro Takahashi
  • Zih Jie Shen
  • Jennifer C Liddle
  • Briana K Dennehey
  • Junyu Xiao
  • Jingyang Wang
  • Hollie Rowlands
  • Roxanne Oshidari
  • Shaofeng Liu
  • Krassimir Yankulov
  • Zhiyun Xu
  • Natalie G Ahn
  • He Leng
  • Di Li
  • Shuqi Li
  • Kirk C Hansen
  • Mair Ea Churchill
  • Xu Zhang
  • Zhongshi Wang
  • Takehiko Kobayashi
  • Jeremy L Balsbaugh
  • Jiawei Xu
  • Mariko Sasaki
  • S Enomoto
  • L F Revers
  • James R Thompson
  • Alex M Port
  • James M Berger
  • Qi Hu
  • Gaofeng Cui
  • S D Johnston
  • Ahmed Fazly
  • Maria Victoria Botuyan
  • Brian A Davies

Detail Information

Publications59

  1. pmc The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae
    S H Meijsing
    Otto Warburg Laboratorium, Max Planck Institut fur Molekulare Genetik, D 14195 Berlin, Germany
    Genes Dev 15:3169-82. 2001
    ..In this study, the MYST family acetyltransferase Sas2 was found to interact with Cac1, the largest subunit of Saccharomyces cerevisiae chromatin assembly factor-I (CAF-I), and with the nucleosome ..
  2. ncbi A role for cell-cycle-regulated histone H3 lysine 56 acetylation in the DNA damage response
    Hiroshi Masumoto
    Chromosome Dynamics Laboratory, Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 436:294-8. 2005
    ..We suggest that the acetylation of histone H3 K56 creates a favourable chromatin environment for DNA repair and that a key component of the DNA damage response is to preserve this acetylation...
  3. pmc Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing
    Shengbing Huang
    Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Proc Natl Acad Sci U S A 102:13410-5. 2005
    ..Furthermore, Rtt106p interacts with CAF-1 physically through Cac1p. These biochemical and genetic data indicate that Rtt106p is a previously uncharacterized histone chaperone connecting S phase to epigenetic inheritance...
  4. pmc Checkpoint functions are required for normal S-phase progression in Saccharomyces cerevisiae RCAF- and CAF-I-defective mutants
    Ellen S Kats
    Ludwig Institute for Cancer Research, Department of Medicine, University of California at San Diego School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0669, USA
    Proc Natl Acad Sci U S A 103:3710-5. 2006
    ..FACS analysis of mutants lacking Asf1 or Cac1 as well as various checkpoint proteins indicated that normal rates of S-phase progression in asf1 mutants have a ..
  5. ncbi A Rad53 kinase-dependent surveillance mechanism that regulates histone protein levels in S. cerevisiae
    Akash Gunjan
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Cell 115:537-49. 2003
    ..Our results argue that Rad53 contributes to genome stability independently of Mec1 by preventing the damaging effects of excess histones both during normal cell cycle progression and in response to DNA damage...
  6. pmc Structural basis for the histone chaperone activity of Asf1
    Christine M English
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, CO 80045, USA
    Cell 127:495-508. 2006
    ..The Asf1-H3/H4 structure suggests a "strand-capture" mechanism whereby the H4 tail acts as a lever to facilitate chromatin disassembly/assembly that may be used ubiquitously by histone chaperones...
  7. pmc Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56
    Robert Driscoll
    Wellcome Trust and Cancer Research U K Gurdon Institute and the Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Science 315:649-52. 2007
    ..These data establish Rtt109p as a member of a new class of histone acetyltransferases and show that its actions are critical for cell survival in the presence of DNA damage during S phase...
  8. pmc Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly
    Qing Li
    Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Cell 134:244-55. 2008
    ..These results reveal a mechanism by which H3K56Ac regulates replication-coupled nucleosome assembly mediated by CAF-1 and Rtt106...
  9. pmc Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete
    Jung Ae Kim
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 106:1151-6. 2009
    ..DSB repair or formation of gamma-H2AX does not depend on either the CAF-1 protein, Cac1, or Asf1...
  10. pmc Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing
    Denise C Krawitz
    Lawrence Berkeley National Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Mol Cell Biol 22:614-25. 2002
    ..Saccharomyces cerevisiae mutants lacking CAF-I subunit genes (CAC1 to CAC3) display reduced heterochromatic gene silencing...
  11. ncbi Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing
    J A Sharp
    Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Curr Biol 11:463-73. 2001
    ..the four HIR genes and nucleosome assembly proteins Asf1p and chromatin assembly factor I (CAF-I, encoded by the CAC1-3 genes)...
  12. pmc A negatively charged residue in place of histone H3K56 supports chromatin assembly factor association but not genotoxic stress resistance
    Judith A Erkmann
    Program in Gene Function and Expression, University of Massachusetts Medical School, 364 Plantation St 506, Worcester, MA 01605, USA
    DNA Repair (Amst) 8:1371-9. 2009
    ..Our studies therefore emphasize how H3 K56ac not only promotes chromatin assembly but also leads to chromosomal malfunction if not removed following histone deposition...
  13. ncbi PCNA connects DNA replication to epigenetic inheritance in yeast
    Z Zhang
    Cold Spring Harbor Laboratory, New York 11724, USA
    Nature 408:221-5. 2000
    ..Thus, PCNA participates in inheritance of both DNA and epigenetic chromatin structures during the S phase of the cell cycle, the latter by at least two mechanisms...
  14. pmc A role for Gcn5 in replication-coupled nucleosome assembly
    Rebecca J Burgess
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Mol Cell 37:469-80. 2010
    ..These results demonstrate that Gcn5 regulates RC nucleosome assembly, in part, by promoting H3 association with CAF-1 via H3 acetylation...
  15. pmc A common telomeric gene silencing assay is affected by nucleotide metabolism
    Marlies P Rossmann
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Cell 42:127-36. 2011
    ..We conclude that metabolic changes caused by the drug employed and certain mutants being studied are incompatible with the use of certain prototrophic markers for TPEV...
  16. pmc A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors
    J S Smith
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Cell Biol 19:3184-97. 1999
    ..Mutations in rpd3 also restored mating competence selectively to sir3Delta MATalpha strains, suggesting restoration of silencing at HMR in a sir3 mutant background...
  17. pmc Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I
    P D Kaufman
    Lawrence Berkeley National Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3206, USA
    Mol Cell Biol 18:4793-806. 1998
    ....
  18. pmc Structural basis for recognition of H3K56-acetylated histone H3-H4 by the chaperone Rtt106
    Dan Su
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA
    Nature 483:104-7. 2012
    ..We show that the Rtt106-(H3-H4)(2) interaction is important for gene silencing and the DNA damage response...
  19. ncbi RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo
    S Enomoto
    Department of Plant Biology, University of Minnesota, St Paul 55108, USA
    Genes Dev 11:358-70. 1997
    ..Furthermore, RLF2 is identical to CAC1, a subunit of yeast chromatin assembly factor-I (yCAF-I) which assembles nucleosomes in vitro...
  20. ncbi The RCAF complex mediates chromatin assembly during DNA replication and repair
    J K Tyler
    Department of Biology and Center for Molecular Genetics, University of California at San Diego, La Jolla 92093 0347, USA
    Nature 402:555-60. 1999
    ....
  21. pmc Saccharomyces cerevisiae chromatin-assembly factors that act during DNA replication function in the maintenance of genome stability
    Kyungjae Myung
    Ludwig Institute for Cancer Research, Cancer Center and Department of Medicine, University of California at San Diego School of Medicine, La Jolla 92093, USA
    Proc Natl Acad Sci U S A 100:6640-5. 2003
    ..These results indicate that coupling of chromatin assembly to DNA replication and DNA repair is critical to maintaining genome stability...
  22. ncbi Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I
    P D Kaufman
    Cold Spring Harbor Laboratory, New York 11724, USA
    Genes Dev 11:345-57. 1997
    ..The CAC1 and CAC2 (chromatin assembly complex) genes encode proteins similar to the p150 and p60 subunits of human CAF-I, ..
  23. pmc The Cac1 subunit of histone chaperone CAF-1 organizes CAF-1-H3/H4 architecture and tetramerizes histones
    Wallace H Liu
    Department of Pharmacology, University of Colorado School of Medicine, Aurora, United States
    elife 5:. 2016
    ..reveal interactions that are essential for CAF-1 function in budding yeast, and importantly indicate that the Cac1 subunit functions as a scaffold within the CAF-1-H3/H4 complex...
  24. pmc RRM3 regulates epigenetic conversions in Saccharomyces cerevisiae in conjunction with Chromatin Assembly Factor I
    Brandon Wyse
    a Department of Molecular and Cellular Biology, University of Guelph, Ontario, Canada
    Nucleus 7:405-14. 2016
    ..We propose that the stalling of DNA replication predisposes to epigenetic conversions and that RRM3 and CAF-I play key roles in this process. ..
  25. pmc More than 10% of yeast genes are related to genome stability and influence cellular senescence via rDNA maintenance
    Kimiko Saka
    National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540 Japan
    Nucleic Acids Res 44:4211-21. 2016
    ....
  26. doi The Histone Chaperone FACT Contributes to DNA Replication-Coupled Nucleosome Assembly
    Jiayi Yang
    State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences and Peking Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
    Cell Rep 14:1128-41. 2016
    ..Importantly, we show that FACT physically associates with Rtt106 and that the acetylation of H3K56, a mark on newly synthesized H3, modulates this interaction. Therefore, FACT collaborates with CAF-1 and Rtt106 in RC nucleosome assembly...
  27. pmc Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis
    Rakesh Kumar Singh
    Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306 4300, USA
    Nat Cell Biol 11:925-33. 2009
    ..Regulated histone proteolysis has major implications for the maintenance of epigenetic marks on chromatin, genomic stability and the packaging of sperm DNA...
  28. pmc The Ddc1-Mec3-Rad17 sliding clamp regulates histone-histone chaperone interactions and DNA replication-coupled nucleosome assembly in budding yeast
    Rebecca J Burgess
    From the Department of Biochemistry and Molecular Biology, Epigenomics Translational Program, Center for Individualized Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota 55905
    J Biol Chem 289:10518-29. 2014
    ..These results support the idea that the 9-1-1 checkpoint protein regulates DNA replication-coupled nucleosome assembly in part through regulating histone-histone chaperone interactions. ..
  29. pmc Systematic triple-mutant analysis uncovers functional connectivity between pathways involved in chromosome regulation
    James E Haber
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Waltham, MA 02454, USA
    Cell Rep 3:2168-78. 2013
    ..We have used this approach to interrogate partially redundant pairs of genes in S. cerevisiae, including ASF1 and CAC1, two histone chaperones...
  30. ncbi MSI1 suppresses hyperactive RAS via the cAMP-dependent protein kinase and independently of chromatin assembly factor-1
    X Zhu
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Curr Genet 38:60-70. 2000
    ..MSI1 appears to provide two distinct roles in chromatin modeling as a component of yCAF-1, and in the inhibition of RAS signaling by modulating PKA...
  31. pmc Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1
    L F Revers
    Depto de Biofísica Centro de Biotecnologia IB UFRGS, Avenida Bento Gonçalves, 9500, Prédio 43421, Campus do Vale, 91501 907 Porto Alegre, RS, Brazil
    Nucleic Acids Res 30:4993-5003. 2002
    ..Amongst 24 interacting clones isolated in a two-hybrid screening, seven could be identified as parts of the RAD2, RLF2 and DBR1 genes...
  32. pmc Histone H3K56 acetylation, CAF1, and Rtt106 coordinate nucleosome assembly and stability of advancing replication forks
    Marta Clemente-Ruiz
    Departamento de Biologia Molecular, Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Consejo Superior de Investigaciones Cientificas, Seville, Spain
    PLoS Genet 7:e1002376. 2011
    ....
  33. pmc Two surfaces on the histone chaperone Rtt106 mediate histone binding, replication, and silencing
    Rachel M Zunder
    Department of Molecular and Cell Biology and California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 109:E144-53. 2012
    ..Our studies described the structural origins of Rtt106 function, identified a conserved histone-binding surface, and defined a critical role for Rtt106:H3K56ac-binding specificity in silencing and replication-coupled nucleosome turnover...
  34. pmc The SCFDia2 ubiquitin E3 ligase ubiquitylates Sir4 and functions in transcriptional silencing
    Rebecca J Burgess
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, MN 55905, USA
    PLoS Genet 8:e1002846. 2012
    ..These results demonstrate that the SCF(Dia2) complex is involved in transcriptional silencing, ubiquitylates Sir4, and regulates transcriptional silencing during the cell cycle...
  35. pmc Effects of Perinuclear Chromosome Tethers in the Telomeric URA3/5FOA System Reflect Changes to Gene Silencing and not Nucleotide Metabolism
    Betty P K Poon
    Faculty of Medicine, Department of Laboratory Medicine and Pathobiology, University of Toronto Toronto, ON, Canada
    Front Genet 3:144. 2012
    ..This is key to understanding relationships between telomere positioning, chromatin silencing, and lifespan...
  36. pmc CAF-1-induced oligomerization of histones H3/H4 and mutually exclusive interactions with Asf1 guide H3/H4 transitions among histone chaperones and DNA
    Wallace H Liu
    Department of Pharmacology, University of Colorado School of Medicine, Mail Stop 8303, PO Box 6511, Aurora, CO 80045, USA
    Nucleic Acids Res 40:11229-39. 2012
    ..Thus, transition of H3/H4 from the Asf1-associated dimer to the DNA-associated tetramer is promoted by CAF-1-induced H3/H4 oligomerization...
  37. ncbi Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair
    Hui Zhou
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, Minnesota 55905, USA
    Biochemistry 45:2852-61. 2006
    ..Together, these data indicate that CAF-1 interacts with H3 methylated at lysine 79 during the processes of epigenetic silencing and DNA repair...
  38. pmc Yeast chromatin assembly complex 1 protein excludes nonacetylatable forms of histone H4 from chromatin and the nucleus
    Lynn Glowczewski
    Department of Genetics, Cell Biology and Development, University of Minnesota, 6 170 MCB Building, 420 Washington Ave SE, Minneapolis, MN 55455, USA
    Mol Cell Biol 24:10180-92. 2004
    ..In addition, our data support a model whereby Cac1p and Kap123 cooperate to ensure that only appropriately acetylated histone H4 proteins are imported into the nucleus...
  39. pmc Separation-of-function mutation in HPC2, a member of the HIR complex in S. cerevisiae, results in derepression of the histone genes but does not confer cryptic TATA phenotypes
    Nidhi Vishnoi
    Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Biochim Biophys Acta 1809:557-66. 2011
    ..This is the first direct demonstration that a separation-of-function mutation exists within the HIR complex...
  40. pmc A conserved patch near the C terminus of histone H4 is required for genome stability in budding yeast
    Yao Yu
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794, USA
    Mol Cell Biol 31:2311-25. 2011
    ....
  41. pmc Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 and HIR proteins in Saccharomyces cerevisiae
    Jessica Lopes da Rosa
    Department of Molecular and Cell Biology, University of California, Berkeley, California 947202, USA
    Genetics 187:9-19. 2011
    ..chromatin immunoprecipitation analyses demonstrated broader distribution of Cse4 outside of centromeres in cac1Δ hir1Δ double mutant cells that lack both CAF-1 and HIR complexes than in either single mutant...
  42. pmc CAC3(MSI1) suppression of RAS2(G19V) is independent of chromatin assembly factor I and mediated by NPR1
    S D Johnston
    Department of Genetics, Cell Biology and Development, University of Minnesota, St Paul, Minnesota 55108, USA
    Mol Cell Biol 21:1784-94. 2001
    ..this mechanism and found that CAC3 suppression of RAS/cAMP signal transduction was independent of either CAC1 or CAC2, subunits required for CAF-I function...
  43. ncbi Methylation of histone H3 lysine-79 by Dot1p plays multiple roles in the response to UV damage in Saccharomyces cerevisiae
    Lindsey J Bostelman
    Department of Biology, Denison University, 213 Talbot Hall, Granville, Ohio 43023, USA
    DNA Repair (Amst) 6:383-95. 2007
    ..The overall results indicate the existence of distinct and separable roles of histone H3 lysine-79 methylation in the response to UV damage, potentially serving to coordinate the various repair processes...
  44. pmc Contribution of CAF-I to anaphase-promoting-complex-mediated mitotic chromatin assembly in Saccharomyces cerevisiae
    Troy A A Harkness
    Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    Eukaryot Cell 4:673-84. 2005
    ..When present in multiple copies, the individual CAF-I genes, CAC1, CAC2, and MSI1, suppress apc5(CA) phenotypes in a CAF-1- and Asf1p-independent manner...
  45. pmc Increased genome instability and telomere length in the elg1-deficient Saccharomyces cerevisiae mutant are regulated by S-phase checkpoints
    Soma Banerjee
    Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Building 49, Room 4A22, Bethesda, MD 20892, USA
    Eukaryot Cell 3:1557-66. 2004
    ....
  46. pmc Regulation of histone deposition proteins Asf1/Hir1 by multiple DNA damage checkpoint kinases in Saccharomyces cerevisiae
    Judith A Sharp
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Genetics 171:885-99. 2005
    ..display defects in telomeric gene silencing, silencing was dramatically reduced in cells lacking both Mec1 and the Cac1 subunit of CAF-1...
  47. pmc Reduction of nucleosome assembly during new DNA synthesis impairs both major pathways of double-strand break repair
    L Kevin Lewis
    Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, USA
    Nucleic Acids Res 33:4928-39. 2005
    ..These results demonstrate that defective assembly of nucleosomes during new DNA synthesis compromises each of the known pathways of DSB repair and that the effects can be indirect consequences of changes in silenced chromatin structure...
  48. pmc Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae
    Tetsushi Iida
    Division of Microbial Genetics, National Institute of Genetics, SOKENDAI, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 24:217-27. 2004
    ..We therefore propose that inheritance of specific epigenetic states of a telomere requires at least two counteracting regulators...
  49. ncbi The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84132, USA
    Mol Cell 22:363-74. 2006
    ..These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process...
  50. pmc Control of translocations between highly diverged genes by Sgs1, the Saccharomyces cerevisiae homolog of the Bloom's syndrome protein
    Kristina H Schmidt
    Department of Biology, University of South Florida, 4202 E Fowler Avenue, SCA110, Tampa, FL 33620, USA
    Mol Cell Biol 26:5406-20. 2006
    ..mutations in the genes encoding the checkpoint proteins Mec3, Rad24, Rad9, or Rfc5, the chromatin assembly factors Cac1 or Asf1, and the DNA helicase Rrm3...
  51. pmc The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin
    Judith A Sharp
    Lawrence Berkeley National Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Genes Dev 17:2356-61. 2003
    ..Sir1 bound to Cac1, a subunit of chromatin assembly factor I (CAF-I), and helped to retain Cac1 at centromeric loci...
  52. pmc Elevated histone expression promotes life span extension
    Jason Feser
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80010, USA
    Mol Cell 39:724-35. 2010
    ..This study indicates that maintenance of the fundamental chromatin structure is critical for slowing down the aging process and reveals that increasing the histone supply extends life span...
  53. pmc Mutual interdependence of MSI1 (CAC3) and YAK1 in Saccharomyces cerevisiae
    Zachary L Pratt
    Department of Biology, North Central College, 30 N Brainard St, Naperville, IL 60540, USA
    J Mol Biol 368:30-43. 2007
    ..Taken together, these data demonstrate a novel interaction between Msi1p and Yak1p in which each protein influences the activity of the other...
  54. ncbi Yeast two-hybrid analysis of the origin recognition complex of Saccharomyces cerevisiae: interaction between subunits and identification of binding proteins
    Kazuya Matsuda
    Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    FEMS Yeast Res 7:1263-9. 2007
    ..We discuss roles of these interactions in functions of ORC...
  55. doi Site specificity of yeast histone acetyltransferase B complex in vivo
    Ana Poveda
    Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, Spain
    FEBS J 275:2122-36. 2008
    ..Finally, the exchange of Lys for Arg at position 12 of histone H4 did not interfere with histone H4 association with the complex, but prevented acetylation on Lys5 by the HAT-B enzyme, in vivo as well as in vitro...
  56. pmc Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae
    Judith A Sharp
    Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Genes Dev 16:85-100. 2002
    ..Finally, CAF-I subunits and Hir1 are enriched at centromeres, indicating that these proteins make a direct contribution to centromeric chromatin structures...
  57. pmc Yeast silent mating type loci form heterochromatic clusters through silencer protein-dependent long-range interactions
    Adriana Miele
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA, USA
    PLoS Genet 5:e1000478. 2009
    ....
  58. pmc Structural analysis of Rtt106p reveals a DNA binding role required for heterochromatin silencing
    Yiwei Liu
    Hefei National Laboratory for Physical Sciences at Microscale and School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, China
    J Biol Chem 285:4251-62. 2010
    ..Our results uncover the structural basis of the diverse functions of Rtt106p and provide new insights into its cellular roles...
  59. pmc The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase
    Susan R Zabaronick
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, 12801 East 17th Ave, Aurora, CO 80010, USA
    Mol Cell Biol 25:652-60. 2005
    ....

Research Grants2

  1. RECOMBINATION MECHANISMS IN YEAST CELL DIFFERENTIATION
    James Haber; Fiscal Year: 2006
    ..abstract_text> ..
  2. Mechanism of in-vitro aging
    LYSLE LEWIS; Fiscal Year: 2007
    ..The proposed research will enhance our understanding of events that occur as a consequence of shortened chromosomes and factors that affect the rate of shortening. [unreadable] [unreadable] [unreadable]..