RAD53

Summary

Gene Symbol: RAD53
Description: serine/threonine/tyrosine protein kinase RAD53
Alias: LSD1, MEC2, SPK1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc FACT prevents the accumulation of free histones evicted from transcribed chromatin and a subsequent cell cycle delay in G1
    Macarena Morillo-Huesca
    Departamento de Genetica, Universidad de Sevilla, Seville, Spain
    PLoS Genet 6:e1000964. 2010
  2. doi Replication stress checkpoint signaling controls tRNA gene transcription
    Vesna C Nguyen
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada
    Nat Struct Mol Biol 17:976-81. 2010
  3. pmc Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases
    Marcus B Smolka
    Ludwig Institute for Cancer Research, La Jolla, CA 92093 0653, USA
    Proc Natl Acad Sci U S A 104:10364-9. 2007
  4. pmc Phosphorylation of Rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forks
    Kristina Herzberg
    Section of Microbiology, University of California, Davis, Davis, CA 95616 8665, USA
    Mol Cell Biol 26:8396-409. 2006
  5. pmc Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis
    Rakesh Kumar Singh
    Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306 4300, USA
    Nat Cell Biol 11:925-33. 2009
  6. pmc Identification of RNR4, encoding a second essential small subunit of ribonucleotide reductase in Saccharomyces cerevisiae
    M Huang
    Verna and Mars McLean Department of Biochemistry, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 17:6105-13. 1997
  7. pmc Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo
    J C Game
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Genetics 151:485-97. 1999
  8. pmc Cell cycle progression in the presence of irreparable DNA damage is controlled by a Mec1- and Rad53-dependent checkpoint in budding yeast
    H Neecke
    Dipartimento di Genetica e di Biologia dei Microrganismi, Via Celoria 26, 20133 Milano, Italy
    EMBO J 18:4485-97. 1999
  9. pmc Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling
    Soo Jung Lee
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Mol Cell Biol 23:6300-14. 2003
  10. pmc Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1
    Christopher Josh Ramey
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center at Fitzsimons, P O Box 6511, Aurora, CO 80045, USA
    Mol Cell Biol 24:10313-27. 2004

Patents

  1. Beta-Tubulin from Acremonium chrysogenum, preparation and use

Scientific Experts

  • Leanne E Wybenga-Groot
  • Rakesh Kumar Singh
  • Ada Collura
  • Robert B Annan
  • Ahmet Koc
  • Hiroo Ogi
  • MICHAEL T FASULLO
  • A Emili
  • Julia M Sidorova
  • Maria Pia Longhese
  • Takashi Hishida
  • Rodrigo Bermejo
  • Ata Ghavidel
  • Roland Chanet
  • Michael Chang
  • Brietta L Pike
  • D Pei
  • David F Stern
  • Soo Jung Lee
  • Vasundhara M Navadgi-Patil
  • N A Koltovaia
  • Takehiko Usui
  • Susan M Gasser
  • Jimmy K Duong
  • Marcus B Smolka
  • Huilin Zhou
  • Marc F Schwartz
  • Bernard P Duncker
  • Carrie Gabrielse
  • John H J Petrini
  • Kenji Shimada
  • Shawn J Szyjka
  • Sheng Hong Chen
  • Oscar M Aparicio
  • James E Haber
  • Frederic D Sweeney
  • Raphael Guerois
  • Francoise Ochsenbein
  • Marie Claude Marsolier-Kergoat
  • David Lydall
  • John R Yates
  • Akash Gunjan
  • Hannah G Blitzblau
  • Ying Chou Chen
  • Giovanna Lucchini
  • Lindsay A Matthews
  • Richard D Kolodner
  • Anna Maria Hegnauer
  • Olga Tsaponina
  • Venkateswarlu Gangavarapu
  • Vladimir I Bashkirov
  • Farokh Dotiwala
  • Ling Ju Lin
  • Thomas Schleker
  • Macarena Morillo-Huesca
  • Rebecca J Burgess
  • Michael C Schultz
  • Jaime Lopez-Mosqueda
  • Genevieve M Vidanes
  • Vesna C Nguyen
  • Teresa M Holzen
  • Willy Aucher
  • David P Toczyski
  • Michael Weinreich
  • Svetlana Makovets
  • Laura Diani
  • Kyungjae Myung
  • Jacob C Harrison
  • Belén Gómez-González
  • Wenyi Feng
  • X Zhao
  • Achille Pellicioli
  • Peter M Burgers
  • Simona Miron
  • Ajai A Prasad
  • Emilie Ma
  • Simona Fiorani
  • James Wohlschlegel
  • Angela T Y Tam
  • Hee Sook Kim
  • Monica Segurado
  • A B Devin
  • Debabrata Biswas
  • Nianhan Jia-Lin Ma
  • Brian Luke
  • Isabelle Morin
  • Anna Travesa
  • Jörg Heierhorst
  • Do Hee Choi
  • Hyun Lee

Detail Information

Publications123 found, 100 shown here

  1. pmc FACT prevents the accumulation of free histones evicted from transcribed chromatin and a subsequent cell cycle delay in G1
    Macarena Morillo-Huesca
    Departamento de Genetica, Universidad de Sevilla, Seville, Spain
    PLoS Genet 6:e1000964. 2010
    ..The G1 delay undergone by spt16 mutants is not mediated by the DNA-damage checkpoint, although the mutation of RAD53, which is otherwise involved in histone degradation, enhances the cell-cycle defects of spt16-197...
  2. doi Replication stress checkpoint signaling controls tRNA gene transcription
    Vesna C Nguyen
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada
    Nat Struct Mol Biol 17:976-81. 2010
    ..Specifically, the sensor kinase Mec1, the signaling adaptor Mrc1 and the transducer kinase Rad53 relay signals that globally repress tRNA gene transcription during unchallenged proliferation and under conditions ..
  3. pmc Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases
    Marcus B Smolka
    Ludwig Institute for Cancer Research, La Jolla, CA 92093 0653, USA
    Proc Natl Acad Sci U S A 104:10364-9. 2007
    ..phosphoproteomics to identify in vivo kinase substrates of the yeast DNA damage checkpoint kinases Mec1, Tel1, and Rad53 (orthologs of human ATR, ATM, and CHK2, respectively)...
  4. pmc Phosphorylation of Rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forks
    Kristina Herzberg
    Section of Microbiology, University of California, Davis, Davis, CA 95616 8665, USA
    Mol Cell Biol 26:8396-409. 2006
    ..These results suggest that Rad55-S2,8,14 phosphorylation activates recombinational repair, allowing for faster recovery after genotoxic stress...
  5. pmc Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis
    Rakesh Kumar Singh
    Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306 4300, USA
    Nat Cell Biol 11:925-33. 2009
    ..Although chromosomal histones are stable, excess (non-chromatin bound) histones are rapidly degraded in a Rad53 (radiation sensitive 53) kinase-dependent manner in Saccharomyces cerevisiae...
  6. pmc Identification of RNR4, encoding a second essential small subunit of ribonucleotide reductase in Saccharomyces cerevisiae
    M Huang
    Verna and Mars McLean Department of Biochemistry, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 17:6105-13. 1997
    ..RNR genes, RNR4 is inducible by DNA-damaging agents through the same signal transduction pathway involving MEC1, RAD53, and DUN1 kinase genes...
  7. pmc Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo
    J C Game
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Genetics 151:485-97. 1999
    ..We find an increased loss of telomeric gene silencing in rad6Delta cac1Delta and rad18Delta cac1Delta double mutants, suggesting that CAF-I and multiple factors in the postreplicative repair pathway influence chromosome structure...
  8. pmc Cell cycle progression in the presence of irreparable DNA damage is controlled by a Mec1- and Rad53-dependent checkpoint in budding yeast
    H Neecke
    Dipartimento di Genetica e di Biologia dei Microrganismi, Via Celoria 26, 20133 Milano, Italy
    EMBO J 18:4485-97. 1999
    ..In fact, Mec1 and Rad53, but not Rad9 and the Rad24 group of checkpoint proteins, are required to delay cell cycle progression in ..
  9. pmc Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling
    Soo Jung Lee
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Mol Cell Biol 23:6300-14. 2003
    Budding yeast Rad53 is an essential protein kinase that is phosphorylated and activated in a MEC1- and TEL1-dependent manner in response to DNA damage...
  10. pmc Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1
    Christopher Josh Ramey
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center at Fitzsimons, P O Box 6511, Aurora, CO 80045, USA
    Mol Cell Biol 24:10313-27. 2004
    ....
  11. pmc Cell cycle- and ribonucleotide reductase-driven changes in mtDNA copy number influence mtDNA Inheritance without compromising mitochondrial gene expression
    Maria A Lebedeva
    Department of Pathology, and Graduate Program in Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Cell Cycle 6:2048-57. 2007
    ..Analysis of rad53 mutant strains also revealed upregulation of mtDNA copy number independent of that resulting from elevated RNR ..
  12. pmc Yeast DNA replication protein Dpb11 activates the Mec1/ATR checkpoint kinase
    Vasundhara M Navadgi-Patil
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 283:35853-9. 2008
    ..we demonstrate that Dpb11 directly activates the Mec1 kinase in phosphorylating the downstream effector kinase Rad53 (human Chk1/2) and DNA bound RPA. However, DNA was not required for Dpb11 to function as an activator...
  13. ncbi Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpoint
    Z Sun
    Department of Biology, Yale University, New Haven, CT 06511, USA
    Science 281:272-4. 1998
    The Rad53 protein kinase of Saccharomyces cerevisiae is required for checkpoints that prevent cell division in cells with damaged or incompletely replicated DNA...
  14. ncbi MEC1-dependent phosphorylation of Rad9p in response to DNA damage
    A Emili
    Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell 2:183-9. 1998
    In budding yeast, DNA damage can activate a checkpoint surveillance system controlled by the RAD9, RAD53, and MEC1 genes, resulting in a delay in cell cycle progression...
  15. pmc The budding yeast Rad9 checkpoint protein is subjected to Mec1/Tel1-dependent hyperphosphorylation and interacts with Rad53 after DNA damage
    J E Vialard
    Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO J 17:5679-88. 1998
    ..Analysis of checkpoint protein interactions after DNA damage revealed that Rad9 physically associates with Rad53.
  16. ncbi Regulation of Saccharomyces Rad53 checkpoint kinase during adaptation from DNA damage-induced G2/M arrest
    A Pellicioli
    Istituto F I R C di Oncologia Molecolare and, Dipartimento di Genetica e di Biologia dei, Microrganismi, Universita degli Studi di Milano, 20133, Milan, Italy
    Mol Cell 7:293-300. 2001
    ..We conclude that activation of the checkpoint kinases in response to a single DNA break is cell cycle regulated and that adaptation is an active process by which these kinases are inactivated...
  17. pmc The ribonucleotide reductase inhibitor Sml1 is a new target of the Mec1/Rad53 kinase cascade during growth and in response to DNA damage
    X Zhao
    Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA
    EMBO J 20:3544-53. 2001
    The evolutionarily conserved protein kinases Mec1 and Rad53 are required for checkpoint response and growth. Here we show that their role in growth is to remove the ribonucleotide reductase inhibitor Sml1 to ensure DNA replication...
  18. pmc Direct kinase-to-kinase signaling mediated by the FHA phosphoprotein recognition domain of the Dun1 DNA damage checkpoint kinase
    Vladimir I Bashkirov
    Section of Microbiology and Center for Genetics and Development, Division of Biological Sciences, University of California, Davis, Davis, California 95616 8665, USA
    Mol Cell Biol 23:1441-52. 2003
    ..It belongs to the Chk2 family of checkpoint kinases, which includes S. cerevisiae Rad53 and Mek1, Schizosaccharomyces pombe Cds1, and human Chk2...
  19. pmc Mdt1, a novel Rad53 FHA1 domain-interacting protein, modulates DNA damage tolerance and G(2)/M cell cycle progression in Saccharomyces cerevisiae
    Brietta L Pike
    St Vincent s Institute of Medical Research, Department of Medicine, St Vincent s Hospital, The University of Melbourne, Fitzroy, Victoria 3065, Australia
    Mol Cell Biol 24:2779-88. 2004
    The Rad53 kinase plays a central role in yeast DNA damage checkpoints. Rad53 contains two FHA phosphothreonine-binding domains that are required for Rad53 activation and possibly downstream signaling...
  20. pmc SPK1 is an essential S-phase-specific gene of Saccharomyces cerevisiae that encodes a nuclear serine/threonine/tyrosine kinase
    P Zheng
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510
    Mol Cell Biol 13:5829-42. 1993
    b>SPK1 was originally discovered in an immunoscreen for tyrosine-protein kinases in Saccharomyces cerevisiae. We have used biochemical and genetic techniques to investigate the function of this gene and its encoded protein...
  21. pmc Rad53-dependent phosphorylation of Swi6 and down-regulation of CLN1 and CLN2 transcription occur in response to DNA damage in Saccharomyces cerevisiae
    J M Sidorova
    Fred Hutchinson Cancer Research Center FHCRC, Basic Sciences Division, Seattle, Washington 98109 1024, USA
    Genes Dev 11:3032-45. 1997
    ..We have shown that after a pulse of DNA damage in G1 with the alkylating agent MMS, there is also a MEC1-, RAD53-, and RAD9-dependent delay in G1...
  22. ncbi The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor
    M Huang
    Howard Hughes Medical Institute, Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    Cell 94:595-605. 1998
    ..Derepression of the Crt1 regulon suppresses the lethality of mec1 and rad53 null alleles and is essential for cell viability during replicative stress...
  23. pmc Recovery from DNA replicational stress is the essential function of the S-phase checkpoint pathway
    B A Desany
    Verna and Marrs McLean Department of Biochemistry, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030 USA
    Genes Dev 12:2956-70. 1998
    b>RAD53 and MEC1 are essential genes required for the transcriptional and cell cycle responses to DNA damage and DNA replication blocks...
  24. ncbi A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools
    X Zhao
    Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, New York 10032 2704, USA
    Mol Cell 2:329-40. 1998
    In Saccharomyces cerevisiae, MEC1 and RAD53 are essential for cell growth and checkpoint function...
  25. pmc RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast
    R Gardner
    Department of Molecular and Cellular Biology, The University of Arizona, PO Box 21016, Tucson, AZ 85721 0106, USA
    EMBO J 18:3173-85. 1999
    ..g. RAD9 and RAD24), those transducing a signal (MEC1, RAD53 and DUN1) or those participating more directly in arrest (PDS1)...
  26. pmc The yeast Sgs1p helicase acts upstream of Rad53p in the DNA replication checkpoint and colocalizes with Rad53p in S-phase-specific foci
    C Frei
    Swiss Institute for Experimental Cancer Research, CH 1066 Epalinges Lausanne, Switzerland
    Genes Dev 14:81-96. 2000
    ..The association of Rad53p with a chromatin subfraction is Sgs1p dependent, suggesting an important role for the helicase in the signal-transducing pathway that monitors replication fork progression...
  27. ncbi Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1
    A Emili
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Mol Cell 7:13-20. 2001
    The evolutionarily conserved yeast checkpoint protein kinase Rad53 regulates cell cycle progression, transcription, and DNA repair in response to DNA damage...
  28. ncbi Suppression of spontaneous chromosomal rearrangements by S phase checkpoint functions in Saccharomyces cerevisiae
    K Myung
    Ludwig Institute for Cancer Research, Cancer Center and Department of Medicine, University of California San Diego School of Medicine, La Jolla, CA 92093, USA
    Cell 104:397-408. 2001
    ..Mutations in Saccharomyces cerevisiae RFC5, DPB11, MEC1, DDC2 MEC3, RAD53, CHK1, PDS1, and DUN1 increased the rate of genome rearrangements up to 200-fold whereas mutations in RAD9, RAD17, ..
  29. ncbi Budding yeast Rad9 is an ATP-dependent Rad53 activating machine
    C S Gilbert
    Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, EN6 3LD, Hertfordshire, United Kingdom
    Mol Cell 8:129-36. 2001
    ..Rad9, whereas the smaller (560 kDa) complex, which forms after DNA damage, contains hyperphosphorylated Rad9 and Rad53. This smaller Rad9 complex is capable of catalyzing phosphorylation and release of active Rad53 kinase, a process ..
  30. pmc Precocious S-phase entry in budding yeast prolongs replicative state and increases dependence upon Rad53 for viability
    Julia M Sidorova
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genetics 160:123-36. 2002
    ..We find that wild-type cells tolerate Swi4-t, whereas checkpoint-deficient rad53-11 cells lose viability within several divisions when Swi4-t is overproduced...
  31. pmc The Dun1 checkpoint kinase phosphorylates and regulates the ribonucleotide reductase inhibitor Sml1
    Xiaolan Zhao
    Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032 2704, USA
    Proc Natl Acad Sci U S A 99:3746-51. 2002
    ..In both yeast and humans, RNR is transcriptionally induced after DNA damage via Mec1/Rad53 (yeast) and ATM/CHK2 (human) checkpoint pathways...
  32. pmc A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1
    Michele Giannattasio
    Dipartimento di Genetica e Biologia dei Microrganismi, Universita degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
    Proc Natl Acad Sci U S A 99:12997-3002. 2002
    ..step-by-step the signaling cascade, which is triggered by DNA lesions and requires the activity of Mec1 and Rad53 kinases...
  33. pmc An N-terminal domain of Dbf4p mediates interaction with both origin recognition complex (ORC) and Rad53p and can deregulate late origin firing
    Bernard P Duncker
    Department of Molecular Biology, University of Geneva, Quai Ernest Ansermet 30, CH 1211 Geneva 4, Switzerland
    Proc Natl Acad Sci U S A 99:16087-92. 2002
    ..Given that the activation of Rad53 kinase in response to the DNA replication checkpoint leads to the release of Dbf4p from an ORC-containing ..
  34. ncbi PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break
    Christophe Leroy
    Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette, Cedex, France
    Mol Cell 11:827-35. 2003
    ..In vivo and in vitro evidence suggests that phosphorylated forms of Ptc2 and Ptc3 specifically bind to the Rad53 FHA1 domain and inactivate Rad53-dependent pathways during adaptation and recovery by dephosphorylating Rad53.
  35. pmc Rad53 checkpoint kinase phosphorylation site preference identified in the Swi6 protein of Saccharomyces cerevisiae
    Julia M Sidorova
    Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, Seattle, WA 98109, USA
    Mol Cell Biol 23:3405-16. 2003
    b>Rad53 of Saccharomyces cerevisiae is a checkpoint kinase whose structure and function are conserved among eukaryotes...
  36. ncbi FHA domain-mediated DNA checkpoint regulation of Rad53
    Marc F Schwartz
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Cell Cycle 2:384-96. 2003
    Saccharomyces cerevisiae Rad53 is a protein kinase central to the DNA damage and DNA replication checkpoint signaling pathways...
  37. ncbi A Rad53 kinase-dependent surveillance mechanism that regulates histone protein levels in S. cerevisiae
    Akash Gunjan
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Cell 115:537-49. 2003
    b>Rad53 and Mec1 are protein kinases required for DNA replication and recovery from DNA damage in Saccharomyces cerevisiae...
  38. pmc A Ddc2-Rad53 fusion protein can bypass the requirements for RAD9 and MRC1 in Rad53 activation
    Soo Jung Lee
    Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA
    Mol Biol Cell 15:5443-55. 2004
    ..In addition, expression of Ddc2-Rad53p in Deltarad24Deltarad17Deltamec3 cells increased cell survival. These results reveal minimal requirements for function of a core checkpoint signaling system...
  39. pmc A Tel1/MRX-dependent checkpoint inhibits the metaphase-to-anaphase transition after UV irradiation in the absence of Mec1
    Michela Clerici
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, P zza della Scienza 2, 20126 Milan, Italy
    Mol Cell Biol 24:10126-44. 2004
    ..Activation of this checkpoint leads to phosphorylation of the downstream checkpoint kinases Rad53 and Chk1, which are required for Tel1-dependent cell cycle arrest, and their adaptor Rad9...
  40. pmc Mechanistically distinct roles for Sgs1p in checkpoint activation and replication fork maintenance
    Lotte Bjergbaek
    Department of Molecular Biology and NCCR, Frontiers in Genetics, University of Geneva, Quai Ernest Ansermet 30, Geneva, Switzerland
    EMBO J 24:405-17. 2005
    ....
  41. ncbi Saccharomyces cerevisiae Rad9 acts as a Mec1 adaptor to allow Rad53 activation
    Frederic D Sweeney
    Centre for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Curr Biol 15:1364-75. 2005
    ..Despite having identified many components of this signaling cascade, the exact mechanisms by which checkpoint kinases are activated after DNA damage, as well as the role of the checkpoint mediators, remain poorly understood...
  42. pmc Replication-independent histone deposition by the HIR complex and Asf1
    Erin M Green
    Lawrence Berkeley National Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Curr Biol 15:2044-9. 2005
    ..These data indicate that the HIR complex and Asf1 proteins function together as a conserved eukaryotic pathway for histone replacement throughout the cell cycle...
  43. pmc Checkpoint functions are required for normal S-phase progression in Saccharomyces cerevisiae RCAF- and CAF-I-defective mutants
    Ellen S Kats
    Ludwig Institute for Cancer Research, Department of Medicine, University of California at San Diego School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0669, USA
    Proc Natl Acad Sci U S A 103:3710-5. 2006
    ..Our data suggest that RCAF defects lead to unstable replication forks that are then stabilized by replication checkpoint proteins, whereas CAF-I defects likely cause different types of DNA damage...
  44. ncbi Cell cycle execution point analysis of ORC function and characterization of the checkpoint response to ORC inactivation in Saccharomyces cerevisiae
    Daniel G Gibson
    Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 2910, USA
    Genes Cells 11:557-73. 2006
    ..We discuss the potential significance of these overlapping checkpoints and the impact of our findings on previously postulated role(s) of ORCs in other cell cycle functions...
  45. pmc Novel role for checkpoint Rad53 protein kinase in the initiation of chromosomal DNA replication in Saccharomyces cerevisiae
    Paul R Dohrmann
    Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado 80045, USA
    Genetics 174:87-99. 2006
    A novel role for Rad53 in the initiation of DNA replication that is independent of checkpoint or deoxynucleotide regulation is proposed...
  46. ncbi Constraining G1-specific transcription to late G1 phase: the MBF-associated corepressor Nrm1 acts via negative feedback
    Robertus A M de Bruin
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Mol Cell 23:483-96. 2006
    ..In both yeasts, MBF represses G1-specific transcription outside of G1 phase. A negative feedback loop involving Nrm1 bound to MBF leads to transcriptional repression as cells exit G1 phase...
  47. pmc Contribution of Trf4/5 and the nuclear exosome to genome stability through regulation of histone mRNA levels in Saccharomyces cerevisiae
    Clara C Reis
    Braun Laboratories, California Institute of Technology, Pasadena, California 91125, USA
    Genetics 175:993-1010. 2007
    ..find that TRF4 shows synthetic genetic interactions with genes that negatively regulate histone levels, including RAD53. Moreover, synthetic lethality of trf4Delta rad53Delta is rescued by reducing histone levels whereas ..
  48. ncbi Checkpoint deficient rad53-11 yeast cannot accumulate dNTPs in response to DNA damage
    Ahmet Koc
    Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir, Turkey
    Biochem Biophys Res Commun 353:527-30. 2007
    ..b>Rad53 is part of a protein kinase regulatory cascade that, conceptually, promotes dNTP accumulation in four ways: (1) it ..
  49. pmc The checkpoint clamp activates Mec1 kinase during initiation of the DNA damage checkpoint
    Jerzy Majka
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Cell 24:891-901. 2006
    ..of the clamp in regulating Mec1, and we delineate how the signal generated by DNA lesions is transduced to the Rad53 effector kinase...
  50. pmc The Saccharomyces cerevisiae 14-3-3 proteins Bmh1 and Bmh2 directly influence the DNA damage-dependent functions of Rad53
    Takehiko Usui
    Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 104:2797-802. 2007
    In this study, we mutated autophosphorylation sites in Rad53 based on their conservation with previously identified autophosphorylation sites in the mammalian Rad53 ortholog, Chk2...
  51. pmc Mechanisms of checkpoint kinase Rad53 inactivation after a double-strand break in Saccharomyces cerevisiae
    Ghislaine Guillemain
    CEA, Direction des Sciences du Vivant, Institut de Biologie et de la Technologies de Saclay, 91191 Gif sur Yvette Cedex, France
    Mol Cell Biol 27:3378-89. 2007
    ..type are required for DNA checkpoint inactivation after a DSB and probably dephosphorylate the checkpoint kinase Rad53. In this article we have investigated further the interactions between Ptc2 and Rad53...
  52. pmc DNA damage checkpoints inhibit mitotic exit by two different mechanisms
    Fengshan Liang
    Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306, USA
    Mol Cell Biol 27:5067-78. 2007
    ..In response to DNA damage, two branches of the DNA damage checkpoint, Chk1 and Rad53, are activated in budding yeast to prevent anaphase entry and mitotic exit, allowing cells more time to repair ..
  53. pmc Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage
    Bryan M O'Neill
    Departments of Chemistry and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 104:9290-5. 2007
    Activation of the checkpoint kinase Rad53 is a critical response to DNA damage that results in stabilization of stalled replication forks, inhibition of late-origin initiation, up-regulation of dNTP levels, and delayed entry to mitosis...
  54. pmc Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1
    Avgi Tsolou
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
    DNA Repair (Amst) 6:1607-17. 2007
    ..This is in accordance with Mrc1-independent Rad53 activation after telomere uncapping...
  55. ncbi Impaired tRNA nuclear export links DNA damage and cell-cycle checkpoint
    Ata Ghavidel
    Ontario Cancer Institute, Division of Signaling Biology, Toronto, Ontario M5G 1L7, Canada
    Cell 131:915-26. 2007
    ..This response requires an intact MEC1- and RAD53-dependent signaling pathway that impedes the nuclear export of intron-containing tRNA via differential ..
  56. pmc Functional interactions between Sae2 and the Mre11 complex
    Hee Sook Kim
    Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Genetics 178:711-23. 2008
    ..We show that Sae2 oligomerizes independently of DNA damage and that oligomerization is required for its regulatory influence on the Mre11 nuclease and checkpoint functions...
  57. ncbi Characterization of the activation domain of the Rad53 checkpoint kinase
    Simona Fiorani
    FIRC Institute of Molecular Oncology Foundation, Milano, Italy
    Cell Cycle 7:493-9. 2008
    b>Rad53 protein, the yeast orthologue of the human checkpoint kinase Chk2, presents two highly conserved phosphorylatable threonine residues (T354 and T358) in the activation domain, whose phosphorylation is critical to allow the ..
  58. pmc Role of the Saccharomyces cerevisiae Rad53 checkpoint kinase in signaling double-strand breaks during the meiotic cell cycle
    Hugo Cartagena-Lirola
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, P zza della Scienza 2, 20126 Milan, Italy
    Mol Cell Biol 28:4480-93. 2008
    ..DNA damage checkpoint response to accidental DSBs during mitosis requires the Rad53 effector kinase, whereas the meiosis-specific Mek1 kinase, together with Red1 and Hop1, mediates the recombination ..
  59. doi Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade
    Hyun Lee
    Genomics Research Center, Academia Sinica, Taipei 115, Taiwan
    Mol Cell 30:767-78. 2008
    ..The Rad53-SCD1 has dual functions in regulating the activation of the Rad53-Dun1 checkpoint kinase cascade but with unknown ..
  60. pmc Separate roles for the DNA damage checkpoint protein kinases in stabilizing DNA replication forks
    Monica Segurado
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms EN6 3LD, United Kingdom
    Genes Dev 22:1816-27. 2008
    ..In budding yeast, the protein kinases Mec1 (ATR) and Rad53 (Chk2) are especially important in this process...
  61. pmc Rad53 regulates replication fork restart after DNA damage in Saccharomyces cerevisiae
    Shawn J Szyjka
    Molecular and Computational Biology Program, University of Southern California, Los Angeles, California 90089, USA
    Genes Dev 22:1906-20. 2008
    Replication fork stalling at a DNA lesion generates a damage signal that activates the Rad53 kinase, which plays a vital role in survival by stabilizing stalled replication forks...
  62. pmc Checkpoint-dependent phosphorylation of Exo1 modulates the DNA damage response
    Isabelle Morin
    Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, Newcastle University, Newcastle upon Tyne, UK
    EMBO J 27:2400-10. 2008
    ..uncapping, Exo1 phosphorylation depends on components of the checkpoint machinery such as Rad24, Rad17, Rad9, Rad53 and Mec1, but is largely independent of Chk1, Tel1 and Dun1...
  63. pmc Maintenance of the DNA-damage checkpoint requires DNA-damage-induced mediator protein oligomerization
    Takehiko Usui
    Laboratory of Chromosome Biology, Sloan Kettering Institute, New York, NY 10065, USA
    Mol Cell 33:147-59. 2009
    ..b>Rad53 activation is unaffected by mutations that impair Rad9 oligomerization, but checkpoint maintenance is lost, ..
  64. pmc The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants
    Belén Gómez-González
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Seville, Spain
    Mol Cell Biol 29:5203-13. 2009
    ..mutants display replication fork progression impairment at actively transcribed chromosomal regions and trigger Rad53 phosphorylation...
  65. pmc Centromere replication timing determines different forms of genomic instability in Saccharomyces cerevisiae checkpoint mutants during replication stress
    Wenyi Feng
    Department of Genome Sciences, University of Washington, Box 355065 Foege Bldg, Room S041, 1705 NE Pacific St, Seattle, Washington 98195, USA
    Genetics 183:1249-60. 2009
    ..different events are responsible for inviability in checkpoint-deficient cells harboring mutations in the mec1 and rad53 genes...
  66. pmc DNA damage signalling prevents deleterious telomere addition at DNA breaks
    Svetlana Makovets
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Nat Cell Biol 11:1383-6. 2009
    ..After DNA damage, MEC1-RAD53-DUN1-dependent phosphorylation of the telomerase inhibitor Pif1 occurs...
  67. pmc The unstructured C-terminal tail of the 9-1-1 clamp subunit Ddc1 activates Mec1/ATR via two distinct mechanisms
    Vasundhara M Navadgi-Patil
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Cell 36:743-53. 2009
    ..Remarkably, small peptides that fuse the two tryptophan-containing motifs together are proficient in activating Mec1...
  68. ncbi Cell cycle-dependent phosphorylation of Rad53 kinase by Cdc5 and Cdc28 modulates checkpoint adaptation
    Thomas Schleker
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    Cell Cycle 9:350-63. 2010
    ..Specifically, higher amounts of damage are needed to activate the downstream checkpoint kinase Rad53 in S-phase cells...
  69. pmc CDC5 inhibits the hyperphosphorylation of the checkpoint kinase Rad53, leading to checkpoint adaptation
    Genevieve M Vidanes
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA
    PLoS Biol 8:e1000286. 2010
    ..Cdc5 overproduction appeared to have little effect on the early steps leading to Rad53 activation...
  70. pmc A role for Gcn5 in replication-coupled nucleosome assembly
    Rebecca J Burgess
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Mol Cell 37:469-80. 2010
    ..These results demonstrate that Gcn5 regulates RC nucleosome assembly, in part, by promoting H3 association with CAF-1 via H3 acetylation...
  71. pmc A strategy for interaction site prediction between phospho-binding modules and their partners identified from proteomic data
    Willy Aucher
    Laboratoire du Métabolisme de l ADN et Réponses aux Génotoxiques, Gif Sur Yvette F 91191, France
    Mol Cell Proteomics 9:2745-59. 2010
    ..The method was validated by a two-hybrid screen carried out using the ForkHead Associated (FHA)1 domain of Rad53, a key protein of Saccharomyces cerevisiae DNA checkpoint, as a bait...
  72. pmc Damage-induced phosphorylation of Sld3 is important to block late origin firing
    Jaime Lopez-Mosqueda
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94158 9001, USA
    Nature 467:479-83. 2010
    ..firing of late replicating origins, and this inhibition requires both Mec1 and the downstream checkpoint kinase Rad53 (Chk2 in humans)...
  73. pmc Ixr1 is required for the expression of the ribonucleotide reductase Rnr1 and maintenance of dNTP pools
    Olga Tsaponina
    Department of Medical Biochemistry and Biophysics, Umea University, Umea, Sweden
    PLoS Genet 7:e1002061. 2011
    The Saccharomyces cerevisiae Dun1 protein kinase is a downstream target of the conserved Mec1-Rad53 checkpoint pathway...
  74. pmc Requirement of replication checkpoint protein kinases Mec1/Rad53 for postreplication repair in yeast
    Venkateswarlu Gangavarapu
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA
    MBio 2:e00079-11. 2011
    ..The Mec1/Rad53-dependent replication checkpoint plays a crucial role in the maintenance of stable and functional replication ..
  75. pmc Saccharomyces cerevisiae Dbf4 has unique fold necessary for interaction with Rad53 kinase
    Lindsay A Matthews
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8S 4K1, Canada
    J Biol Chem 287:2378-87. 2012
    ..During the replication checkpoint, Saccharomyces cerevisiae Dbf4 is phosphorylated in a Rad53-dependent manner, and this, in turn, inhibits initiation of replication at late origins...
  76. pmc An N-terminal acidic region of Sgs1 interacts with Rpa70 and recruits Rad53 kinase to stalled forks
    Anna Maria Hegnauer
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    EMBO J 31:3768-83. 2012
    ..However, the Sgs1 R1 domain is a target of Mec1 kinase, deletion of which compromises Rad53 activation on HU...
  77. pmc DNA replication checkpoint signaling depends on a Rad53-Dbf4 N-terminal interaction in Saccharomyces cerevisiae
    Ying Chou Chen
    Laboratory of Genome Integrity and Tumorigenesis, Van Andel Research Institute, Grand Rapids, MI 49503, USA
    Genetics 194:389-401. 2013
    ..b>Rad53 kinase is a central effector of the replication checkpoint and both binds to and phosphorylates Dbf4 to prevent ..
  78. ncbi Mitotic checkpoint genes in budding yeast and the dependence of mitosis on DNA replication and repair
    T A Weinert
    Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721
    Genes Dev 8:652-65. 1994
    ..The MEC2 gene proves to be identical to the RAD53 gene...
  79. ncbi A DNA damage response pathway controlled by Tel1 and the Mre11 complex
    T Usui
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, 560 0043, Osaka, Japan
    Mol Cell 7:1255-66. 2001
    ..In mitotic cells, the Tel1-Mre11 complex pathway triggers Rad53 activation and its interaction with Rad9, whereas in meiosis it acts via Rad9 and the Rad53 paralog Mre4/Mek1...
  80. ncbi Rad9 phosphorylation sites couple Rad53 to the Saccharomyces cerevisiae DNA damage checkpoint
    Marc F Schwartz
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    Mol Cell 9:1055-65. 2002
    ..for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1. DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9...
  81. ncbi Activation of the checkpoint kinase Rad53 by the phosphatidyl inositol kinase-like kinase Mec1
    Jia Lin Ma
    Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Biol Chem 281:3954-63. 2006
    Saccharomyces cerevisiae Rad53, the ortholog of mammalian Chk2, is an essential protein kinase in DNA damage and DNA replication checkpoint pathways...
  82. pmc A Dbf4p BRCA1 C-terminal-like domain required for the response to replication fork arrest in budding yeast
    Carrie Gabrielse
    Laboratory of Chromosome Replication, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA
    Genetics 173:541-55. 2006
    ..Rad53p likely directly phosphorylated Dbf4p in response to RF arrest and Dbf4p was required for Rad53p abundance. Rad53p and Dbf4p therefore cooperated to coordinate a robust cellular response to RF arrest...
  83. ncbi A domain of Rad9 specifically required for activation of Chk1 in budding yeast
    Richard T Blankley
    School of Biological Sciences, University of Manchester, G38 Stopford Building, Oxford Rd, Manchester, M13 9PT, UK
    J Cell Sci 117:601-8. 2004
    ..is to link the activity of the Mec1 kinase to the activation of two parallel signalling pathways dependent on the Rad53 and Chk1 kinases. The mechanisms by which Rad9 interacts with, and activates, Rad53 are well understood...
  84. pmc Mechanism of Dun1 activation by Rad53 phosphorylation in Saccharomyces cerevisiae
    Sheng Hong Chen
    Division of Biological Sciences, Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla 92093 0653, USA
    J Biol Chem 282:986-95. 2007
    ..Using this assay, we confirmed the genetic basis of Dun1 activation. Rad53 was found to be directly responsible for Dun1 activation...
  85. pmc ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast
    Hannah G Blitzblau
    Whitehead Institute for Biomedical Research, Cambridge, United States
    elife 2:e00844. 2013
    ..DOI:http://dx.doi.org/10.7554/eLife.00844.001. ..
  86. ncbi Two alternative cell cycle checkpoint pathways differentially control DNA damage-dependent induction of MAG1 and DDI1 expression in yeast
    Y Zhu
    Department of Microbiology and Immunology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada
    Mol Genet Genomics 266:436-44. 2001
    ..We previously reported that MAG1 induction was abolished in pol2 and rad53 mutants, but not in the mec1-1 mutant...
  87. pmc Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiae
    Kyungjae Myung
    Ludwig Institute for Cancer Research, Cancer Center, and Department of Medicine, University of California at San Diego School of Medicine, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 99:4500-7. 2002
    ..were found to be important members of all the S-phase checkpoints in suppressing genome instability, whereas RAD53 only seemed to play a role in the intra-S checkpoints...
  88. pmc A mutation in Dbf4 motif M impairs interactions with DNA replication factors and confers increased resistance to genotoxic agents
    Angela E Varrin
    Department of Biology, University of Waterloo, Ontario, Canada
    Mol Cell Biol 25:7494-504. 2005
    ..Previously, a 186-amino-acid Dbf4 region that mediates interactions with both the origin recognition complex and Rad53 was identified...
  89. pmc Stimulation of sister chromatid exchanges and mutation by aflatoxin B1-DNA adducts in Saccharomyces cerevisiae requires MEC1 (ATR), RAD53, and DUN1
    Michael Fasullo
    Ordway Research Institute, Center for Medical Sciences, Department of Biomedical Sciences, State University of New York at Albany, 150 New Scotland Avenue, Albany, New York 12209, USA
    Mol Carcinog 47:608-15. 2008
    ..AFB(1) exposure increases frequencies of RAD51-dependent unequal sister chromatid exchange (SCE) and activates Rad53 (CHK2). We found that MEC1, RAD53, and DUN1 are required for both AFB(1)-associated mutation and SCE...
  90. doi Location-specific functions of the two forkhead-associated domains in Rad53 checkpoint kinase signaling
    Angela T Y Tam
    St Vincent s Institute of Medical Research and Department of Medicine SVH, The University of Melbourne, 9 Princes Street, Fitzroy, VIC 3065, Australia
    Biochemistry 47:3912-6. 2008
    ..The Saccharomyces cerevisiae checkpoint kinase Rad53 contains two phosphothreonine-binding forkhead-associated (FHA) domains...
  91. doi The mutation of a novel Saccharomyces cerevisiae SRL4 gene rescues the lethality of rad53 and lcd1 mutations by modulating dNTP levels
    Do Hee Choi
    Department of Biological Sciences, College of Natural Science, Inha University, Incheon 402 751, Republic of Korea
    J Microbiol 46:75-80. 2008
    ..Furthermore, srl4Delta was shown to suppress the lethality of mutations of the essential S phase checkpoint genes, RAD53 and LCD1...
  92. pmc UV but not X rays stimulate homologous recombination between sister chromatids and homologs in a Saccharomyces cerevisiae mec1 (ATR) hypomorphic mutant
    Michael Fasullo
    Ordway Research Institute, Albany, NY 12208, USA
    Mutat Res 648:73-81. 2008
    ..Over-expression of Rad53 in mec1-21 reduced UV-associated recombination but did not suppress the defect in X-ray-associated recombination...
  93. pmc Asf1 can promote trimethylation of H3 K36 by Set2
    Ling Ju Lin
    Department of Biochemistry, University of Alberta, MSB 5 76, Edmonton, Alberta, Canada T6G 2H7
    Mol Cell Biol 30:1116-29. 2010
    ..This function of Asf1 promotes the switch from di- to trimethylation of H3 K36 at that gene. These results support the view that Set2 function in chromatin metabolism can intimately involve histone chaperone Asf1...
  94. pmc Genetic interaction of RAD53 protein kinase with histones is important for DNA replication
    Teresa M Holzen
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, USA
    Cell Cycle 9:4735-47. 2010
    Studies in budding yeast suggest the protein kinase Rad53 plays novel roles in controlling initiation of DNA replication and in maintaining cellular histone levels, and these roles are independent of Rad53-mediated regulation of the ..
  95. pmc RAD9, RAD17, and RAD24 are required for S phase regulation in Saccharomyces cerevisiae in response to DNA damage
    A G Paulovich
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Genetics 145:45-62. 1997
    We have previously shown that a checkpoint dependent on MEC1 and RAD53 slows the rate of S phase progression in Saccharomyces cerevisiae in response to alkylation damage...
  96. pmc A role for DNA primase in coupling DNA replication to DNA damage response
    F Marini
    Dipartimento di Genetica e di Biologia dei Microrganismi, Universita degli Studi di Milano, Italy
    EMBO J 16:639-50. 1997
    ..Altogether, our results suggest that DNA primase plays an essential role in a subset of the Rad53p-dependent checkpoint pathways controlling cell cycle progression in response to DNA damage...
  97. pmc Rfc5, a replication factor C component, is required for regulation of Rad53 protein kinase in the yeast checkpoint pathway
    K Sugimoto
    Department of Molecular Biology, Faculty of Science, Nagoya University, Japan
    Mol Cell Biol 17:5905-14. 1997
    ..The phosphorylation of the essential central transducer, Rad53 protein kinase, is reduced in response to DNA damage in rfc5-1 mutants during the S phase...
  98. pmc RAD53 regulates DBF4 independently of checkpoint function in Saccharomyces cerevisiae
    P R Dohrmann
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Genetics 151:965-77. 1999
    ..The lsd1 complementation group contained a new allele of RAD53, which was designated rad53-31...
  99. ncbi II. Structure and specificity of the interaction between the FHA2 domain of Rad53 and phosphotyrosyl peptides
    P Wang
    Departments of Chemistry and Biochemistry, The Ohio State Biochemistry Program, and Campus Chemical Instrument Center, The Ohio State University, Columbus, OH, 43210, USA
    J Mol Biol 302:927-40. 2000
    ..Recently we have determined the first structure of an FHA domain, FHA2 from the yeast protein Rad53, and demonstrated that FHA2 binds to a pTyr-containing peptide (826)EDI(pY)YLD(832) from Rad9, with a moderate ..
  100. pmc Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae
    T Ohya
    Department of Biochemistry and Molecular Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Nucleic Acids Res 28:3846-52. 2000
    ..Furthermore, the restrictive temperature of double mutants carrying Deltadpb4 and dpb2-1, rad53-1 or rad53-21 is lower than in the corresponding single mutants...
  101. pmc Asf1 links Rad53 to control of chromatin assembly
    F Hu
    Verna and Mars McLean Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 15:1061-6. 2001
    Yeast defective in the checkpoint kinase Rad53 fail to recover from transient DNA replication blocks and synthesize intact chromosomes. The effectors of Rad53 relevant to this recovery process are unknown...

Research Grants2

  1. RADIATION INDUCTION OF GENOMIC REARRANGEMENTS IN YEAST
    Michael Fasullo; Fiscal Year: 2004
    ..abstract_text> ..
  2. Activating liver carcinogens in yeast by expressing CYP450 polymorphisms
    Michael Fasullo; Fiscal Year: 2007
    ..induction of DNA repair genes after AFB1 exposure, and AFB1-associated activation of checkpoint protein Rad53 (CHK2)...

Patents1

  1. Beta-Tubulin from Acremonium chrysogenum, preparation and use
    Patent Number: EP0610842-A2; Date:1994-08-17