Genomes and Genes
Gene Symbol: PPH21
Description: phosphoprotein phosphatase 2A catalytic subunit PPH21
Species: Saccharomyces cerevisiae S288c
- Intranuclear function for protein phosphatase 2A: Pph21 and Pph22 are required for rapamycin-induced GATA factor binding to the DAL5 promoter in yeastIsabelle Georis
Institut de Recherches Microbiologiques J M Wiame, Laboratoire de Microbiologie, Universite Libre de Bruxelles, B1070 Brussels, Belgium
Mol Cell Biol 31:92-104. 2011Protein phosphatase 2A (PP2A), a central Tor pathway phosphatase consisting of a catalytic subunit (Pph21 or Pph22), a scaffold subunit (Tpd3), and one of two regulatory subunits (Cdc55 or Rts1), has been repeatedly shown to play ..
- Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2Christine Van Hoof
Afdeling Biochemie, Faculteit Geneeskunde, Campus Gasthuisberg O and N, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
Biochem J 386:93-102. 2005..Ypa1 interacts specifically with Pph3, Sit4 and Ppg1, whereas Ypa2 binds to Pph21 and Pph22...
- Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeastValentina Rossio
Department of Biology, Brandeis University, Waltham, MA 02454, USA
J Cell Biol 193:445-54. 2011..Our analysis defines the long-sought molecular function for the zillion different screens family proteins and reveals the importance of the regulation of PP2A localization for proper mitotic progression...
- The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphataseSidonie Wicky
Department of Molecular, Cell, and Developmental Biology, Univ of California, Santa Cruz, CA 95064, USA
Mol Biol Cell 22:20-32. 2011..Together, these observations define a role for the Zds proteins in controlling specific functions of PP2A(Cdc55) and suggest that upstream signals that regulate PP2A(Cdc55) may play an important role in controlling entry into mitosis...
- Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domainKimberly Yasutis
Departments of Biology and Cell Biology, University of Virginia, Charlottesville, VA 22904, USA
Mol Biol Cell 21:4373-86. 2010..This negative regulation requires the CDC55 gene. Together these data indicate that the Cdc55p/Zds2p module has a function in the regulation of the Swe1p-dependent G2/M checkpoint...
- A phosphatase threshold sets the level of Cdk1 activity in early mitosis in budding yeastStacy L Harvey
Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
Mol Biol Cell 22:3595-608. 2011..These results define a new role for PP2A(Cdc55) and reveal a systems-level mechanism by which dynamically opposed kinase and phosphatase activities can modulate signal strength...
- Yeast endosulfines control entry into quiescence and chronological life span by inhibiting protein phosphatase 2ASéverine Bontron
Department of Biology, Division of Biochemistry, University of Fribourg, 1700 Fribourg, Switzerland
Cell Rep 3:16-22. 2013..These results close a gap in our perception of and delineate a role for PP2A(Cdc55) in TORC1-/PKA-mediated regulation of quiescence and chronological life span...
- Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1Gayatri Pal
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
J Cell Biol 180:931-45. 2008..Because casein kinase 1 is associated with sites of polar growth, it may regulate Mih1 as part of a signaling mechanism that links successful completion of growth-related events to cell cycle progression...
- Generation of active protein phosphatase 2A is coupled to holoenzyme assemblyHans Hombauer
Department of Medical Biochemistry, Max F Perutz Laboratories, Medical University of Vienna, Vienna, Austria
PLoS Biol 5:e155. 2007..We propose a novel model of PP2A biogenesis in which a tightly controlled activation cascade protects cells from untargeted activity of the free catalytic PP2A subunit...
- Rapamycin activates Tap42-associated phosphatases by abrogating their association with Tor complex 1Gonghong Yan
Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
EMBO J 25:3546-55. 2006..In addition, our data support a model in which rapamycin acts not by inhibiting the kinase activity of Tor but by disrupting its interaction with downstream targets...
- Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiaeYanchang Wang
Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL 32306, USA
Mol Biol Cell 17:80-9. 2006..Genes that encode the B-regulatory subunit (Cdc55) and the three catalytic subunits (Pph21, Pph22, and Pph3) of phosphatase 2A (PP2A) were isolated...
- The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexesYin Zheng
Department of Pharmacology, University of Pittsburgh School of Medicine, PA 15213, USA
Mol Biol Cell 16:2119-27. 2005....
- Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeastY Jiang
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
EMBO J 18:2782-92. 1999..of a protein, Tap42, with two different protein phosphatase catalytic subunits; one encoded redundantly by PPH21 and PPH22, and one encoded by SIT4...
- Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genesA A Sneddon
Department of Biochemistry, The University, Dundee, UK
EMBO J 9:4339-46. 1990Two genes (PPH21 and PPH22) encoding the yeast homologues of protein serine-threonine phosphatase 2A have been cloned from a Saccharomyces cerevisiae genomic library using a rabbit protein phosphatase 2A cDNA as a hybridization probe...
- Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiaeY Wang
Department of Biology, University of Virginia, Charlottesville 22903, USA
Mol Cell Biol 17:620-6. 1997..Finally, cdc55::LEU2 suppresses the temperature sensitivity of cdc20-1, suggesting additional roles for CDC55 in mitosis...
- Molecular genetic analysis of Rts1p, a B' regulatory subunit of Saccharomyces cerevisiae protein phosphatase 2AY Shu
Department of Biology, Syracuse University, New York 13244, USA
Mol Cell Biol 17:3242-53. 1997..Genetic analyses show that the role played by Rts1p in PP2A regulation is distinctly different from that played by the other known variable B regulatory subunit, Cdc55p, a protein recently implicated in checkpoint control regulation...
- The Saccharomyces cerevisiae phosphotyrosyl phosphatase activator proteins are required for a subset of the functions disrupted by protein phosphatase 2A mutationsC Van Hoof
Afdeling Biochemie, Departement Geneeskunde, Katholieke Universiteit Leuven, Herestraat 49, Leuven, B 3000, Belgium
Exp Cell Res 264:372-87. 2001..Therefore, Ypa does not seem to play a role in the regulation of PP2A at this stage of the cell cycle. These results imply that Ypa regulates a specific subset of PP2A functions, possibly by controlling the subunit composition of PP2A...
- Loss of a protein phosphatase 2A regulatory subunit (Cdc55p) elicits improper regulation of Swe1p degradationH Yang
Department of Biology, Syracuse University, Syracuse, New York 13244, USA
Mol Cell Biol 20:8143-56. 2000..We also present evidence indicating that, in cdc55-null cells, misregulated PP2A phosphatase activity is the cause of both the ectopic stabilization of Swe1p and the production of the morphologically abnormal phenotype...
- Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1pH Wei
Department of Biochemistry and Winship Cancer Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
J Biol Chem 276:1570-7. 2001..Taken together, our results indicate that methylation and phosphorylation may be mechanisms by which the cell dynamically regulates PP2A complex formation and function...
- Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatasesHuamin Wang
Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Mol Biol Cell 14:4342-51. 2003In Saccharomyces cerevisiae, Pph21 and Pph22 are the two catalytic subunits of type 2A phosphatase (PP2Ac), and Sit4 is a major form of 2A-like phosphatase...
- The Tap42-protein phosphatase type 2A catalytic subunit complex is required for cell cycle-dependent distribution of actin in yeastHuamin Wang
Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Mol Cell Biol 23:3116-25. 2003..In addition, we provide evidence that PP2A activity plays a negative role in controlling the actin cytoskeleton and, possibly, in regulation of the G(2)/M transition of the cell cycle...
- The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosisF C Lin
Cold Spring Harbor Laboratory, NY 11724, USA
EMBO J 14:2745-59. 1995We have prepared a temperature-sensitive Saccharomyces cerevisiae type 2A phosphatase (PP2A) mutant, pph21-102...
- Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesisH Ronne
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden
Mol Cell Biol 11:4876-84. 1991We have cloned three genes for protein phosphatases in the yeast Saccharomyces cerevisiae. Two of the genes, PPH21 and PPH22, encode highly similar proteins that are homologs of the mammalian protein phosphatase 2A (PP2A), while the ..
- Promotion of Cell Viability and Histone Gene Expression by the Acetyltransferase Gcn5 and the Protein Phosphatase PP2A in Saccharomyces cerevisiaeEmily L Petty
Division of Biological Sciences, Molecular Biology, University of California, San Diego Moores Cancer Center, La Jolla, California 92093
Genetics 203:1693-707. 2016..These data highlight the potential significance of dynamic phosphorylation and dephosphorylation of these deeply conserved histone residues for cell viability. ..
- Deletion of conserved protein phosphatases reverses defects associated with mitochondrial DNA damage in Saccharomyces cerevisiaeGörkem Garipler
Department of Molecular Biology and Genetics and School of Medicine, Koc University, Sariyer, Istanbul, 34450 Turkey
Proc Natl Acad Sci U S A 111:1473-8. 2014..Our work highlights the important role that nutrient-responsive signaling pathways can play in determining the response to mitochondrial dysfunction. ..
- Protein kinase C controls binding of Igo/ENSA proteins to protein phosphatase 2A in budding yeastVu Thai
University of California, Santa Cruz, United States
J Biol Chem . 2017..Together, the data suggest that Pkc1 controls PP2ACdc55 by multiple overlapping mechanisms...
- A novel Sit4 phosphatase complex is involved in the response to ceramide stress in yeastAlexandra Woodacre
Department of Genetics, University of Leicester, Leicester, UK
Oxid Med Cell Longev 2013:129645. 2013..Therefore, Kti12p may play a similar secondary role in the ceramide response. This evidence points to a novel Sit4-dependent regulatory mechanism in response to ceramide stress. ..
- Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpointValentina Rossio
Department of Biology and Rosenstiel Basic Biomedical Sciences Research Center, Brandeis University, 415 South Street, Waltham, MA 02454, USA
J Cell Sci 126:4396-405. 2013..Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by Zds1 family proteins is important for tight control of SAC and mitotic progression...
- Budding yeast greatwall and endosulfines control activity and spatial regulation of PP2A(Cdc55) for timely mitotic progressionMaría Angeles Juanes
Centre de Recherche en Biochimie Macromoleculaire, Montpellier, France
PLoS Genet 9:e1003575. 2013..However, this conserved module is adapted to account for differences in the regulation of mitotic entry in different organisms. ..
- The protein phosphatase Siw14 controls caffeine-induced nuclear localization and phosphorylation of Gln3 via the type 2A protein phosphatases Pph21 and Pph22 in Saccharomyces cerevisiaeMinori Numamoto
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan and Yeast Genetic Resources Laboratory, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
J Biochem 157:53-64. 2015..although the nuclear localization of Gln3 is known to be induced by activation of the type 2A phosphatases (PP2As) Pph21 and Pph22, and the type 2A-related phosphatase Sit4...
- Separase biosensor reveals that cohesin cleavage timing depends on phosphatase PP2A(Cdc55) regulationGilad Yaakov
Department of Physiology, University of California San Francisco, CA 94158, USA
Dev Cell 23:124-36. 2012..Thus, separase acts directly on Scc1 and also indirectly, through inhibition of PP2A(Cdc55), to stimulate cohesin cleavage, providing a feedforward loop that may contribute to a robust and timely anaphase...
- Oma1 Links Mitochondrial Protein Quality Control and TOR Signaling To Modulate Physiological Plasticity and Cellular Stress ResponsesIryna Bohovych
Department of Biochemistry, University of Nebraska Lincoln, Lincoln, Nebraska, USA Redox Biology Center, University of Nebraska Lincoln, Lincoln, Nebraska, USA
Mol Cell Biol 36:2300-12. 2016....
- The ceramide-activated protein phosphatase Sit4p controls lifespan, mitochondrial function and cell cycle progression by regulating hexokinase 2 phosphorylationAntónio Daniel Barbosa
b IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
Cell Cycle 15:1620-30. 2016..The overall results suggest that Hxk2p functions downstream of Sit4p in the control of cell cycle, mitochondrial function, oxidative stress resistance and chronological lifespan. ..
- Glucose-induced posttranslational activation of protein phosphatases PP2A and PP1 in yeastDries Castermans
Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KULeuven, Belgium
Cell Res 22:1058-77. 2012..Rts1 interacted with both Pph21 and Glc7 under different conditions and these interactions were Reg1 dependent...
- TORC2 signaling is antagonized by protein phosphatase 2A and the Far complex in Saccharomyces cerevisiaeTammy Pracheil
Department of Biological Sciences, University of New Orleans, New Orleans, Louisiana 70148, USA
Genetics 190:1325-39. 2012..a member of the Far3-7-8-9-10-11 complex involved in pheromone-induced cell cycle arrest, interacts with Tpd3 and Pph21, conserved components of PP2A, and deletions of components of the Far3-7-8-9-10-11 complex and PP2A rescue growth ..
- PP4 dephosphorylates Maf1 to couple multiple stress conditions to RNA polymerase III repressionAndrew J Oler
HHMI, Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT, USA
EMBO J 31:1440-52. 2012....
- Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathwaysYidi Sun
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
Mol Biol Cell 23:2388-98. 2012..We propose that this mechanism serves as the basis for how Orm phosphoregulation controls sphingolipid biosynthesis in response to stress in a kinetically coupled manner...
- PP2A(Cdc55) regulates G1 cyclin stabilityPaula McCourt
Venenum Biodesign, Genesis Biotechnology Group, Hamilton, NJ, USA
Cell Cycle 12:1201-10. 2013..In sum, our results indicate that PP2A is absolutely required to maintain G1-S cyclin levels through modulating their phosphorylation status, an event necessary to properly transit through the cell cycle...
- Role of protein phosphatase 2A in the control of glycogen metabolism in yeastJ Clotet
Departament de Bioquimica i Biologia Molecular, Facultat de Veterinaria, Universitat Autonoma de Barcelona, Spain
Eur J Biochem 229:207-14. 1995The yeast homologues of mammalian protein phosphatase 2A (PP2A) are encoded by two genes, PPH21 and PPH22...
- Overexpression of yeast PAM1 gene permits survival without protein phosphatase 2A and induces a filamentous phenotypeG Z Hu
Ludwig Institute for Cancer Research, Uppsala Biomedical Center, Sweden
J Biol Chem 269:3429-35. 1994..In the yeast Saccharomyces cerevisiae, the catalytic subunit of PP2A is encoded by two duplicated genes, PPH21 and PPH22. A third related gene, PPH3, also contributes some PP2A activity...
- The phosphotyrosyl phosphatase activator, Ncs1p (Rrd1p), functions with Cla4p to regulate the G(2)/M transition in Saccharomyces cerevisiaeD A Mitchell
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
Mol Cell Biol 21:488-500. 2001..Strains lacking both NCS1 and NOH1 were inviable and arrested as unbudded cells, implying that PTPA function is required for proper G(1) progression...
- A series of double disruptants for protein phosphatase genes in Saccharomyces cerevisiae and their phenotypic analysisNaoko Sakumoto
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
Yeast 19:587-99. 2002..Four double disruptants exhibited synthetic phenotypes in addition to eight single ones: the pph21 pph22 double disruptant showed slow growth on complete medium, as did the sit4 and yvh1 single ones...
- Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiaeIvan Munoz
Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona
Yeast 20:157-69. 2003..The possible relationship between the known functions of these suppressor genes and the progress through the G(1)-S transition is discussed...
- Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis IChristian G Riedel
Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
Nature 441:53-61. 2006..Our data are consistent with the notion that efficient cleavage of Rec8 requires phosphorylation of cohesin and that this is blocked by PP2A at meiosis I centromeres...
- Ensemble modeling for analysis of cell signaling dynamicsLars Kuepfer
Institute of Molecular Systems Biology, ETH Zurich, 8092 Zurich, Switzerland
Nat Biotechnol 25:1001-6. 2007..Beyond revising our picture of TOR signaling, we expect ensemble modeling to help elucidate other insufficiently characterized cellular circuits...
- The yeast Tor signaling pathway is involved in G2/M transition via polo-kinaseAkio Nakashima
Biosignal Research Center, Kobe University, Kobe, Japan
PLoS ONE 3:e2223. 2008..The C-terminal polo-box domain of Cdc5 has an inhibitory role in nuclear translocation. Taken together, our results indicate a novel function of Tor in the regulation of cell cycle and proliferation...
- Rapamycin-induced Gln3 dephosphorylation is insufficient for nuclear localization: Sit4 and PP2A phosphatases are regulated and function differentlyJennifer J Tate
Department of Molecular Sciences, University of Tennessee, Memphis, Tennessee 38163, USA
J Biol Chem 284:2522-34. 2009..In contrast, Rap-induced nuclear Gln3 localization absolutely requires all four PP2A components (Pph21/22, Tpd3, Cdc55, and Rts1)...
- The protein phosphatase 2A functions in the spindle position checkpoint by regulating the checkpoint kinase Kin4Leon Y Chan
David H Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Genes Dev 23:1639-49. 2009....
- Distinct phosphatase requirements and GATA factor responses to nitrogen catabolite repression and rapamycin treatment in Saccharomyces cerevisiaeJennifer J Tate
Department of Molecular Sciences, University of Tennessee, Memphis, Tennessee 38163, USA
J Biol Chem 285:17880-95. 2010..We previously demonstrated that the Sit4 and Pph21/22-Tpd3-Cdc55/Rts1 requirements for nuclear Gln3 localization differ...