NUP120

Summary

Gene Symbol: NUP120
Description: Nup120p
Alias: RAT2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene
    C V Heath
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Cell Biol 131:1677-97. 1995
  2. pmc ER membrane-bending proteins are necessary for de novo nuclear pore formation
    T Renee Dawson
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Cell Biol 184:659-75. 2009
  3. pmc The structure of the scaffold nucleoporin Nup120 reveals a new and unexpected domain architecture
    Nina C Leksa
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Structure 17:1082-91. 2009
  4. pmc Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex
    Hyuk Soo Seo
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:14281-6. 2009
  5. pmc Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes
    Benoit Palancade
    Institut Curie, Centre de Recherche, and Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique, F 75248 Paris, France
    Mol Biol Cell 18:2912-23. 2007
  6. pmc Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution
    Anne Chadrin
    Institut Jacques Monod, UMR 7592, Centre National de la Recherche Scientifique Université Paris Diderot, 75013 Paris, France
    J Cell Biol 189:795-811. 2010
  7. pmc The integral membrane protein Pom34p functionally links nucleoporin subcomplexes
    Mi Miao
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232 8240, USA
    Genetics 172:1441-57. 2006
  8. ncbi Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p
    Huanhuan Gao
    Department of Physiology, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 278:25331-40. 2003
  9. pmc Structure-function mapping of a heptameric module in the nuclear pore complex
    Javier Fernandez-Martinez
    Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY 10065, USA
    J Cell Biol 196:419-34. 2012
  10. pmc Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins
    Malik Lutzmann
    BZH Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    EMBO J 21:387-97. 2002

Scientific Experts

  • Benoit Palancade
  • Svetlana Dokudovskaya
  • E Fabre
  • Craig B Bennett
  • Javier Fernandez-Martinez
  • Susan R Wente
  • S Siniossoglou
  • Amanda K Casey
  • Ed Hurt
  • Seung Joong Kim
  • Brian T Chait
  • Andrej Sali
  • Michael P Rout
  • Xiaoping Liu
  • Jean Baptiste Leducq
  • Akira Hayakawa
  • Malik Lutzmann
  • Shana C Kerr
  • Nayan J Sarma
  • William T Yewdell
  • Gunter Blobel
  • Anne Chadrin
  • William H Light
  • Dirk Flemming
  • Hyuk Soo Seo
  • T Renee Dawson
  • Nina C Leksa
  • Wei Yao
  • Cristina González-Aguilera
  • Nai Jung Hung
  • Rune Thomsen
  • E Hurt
  • John J Scarcelli
  • Tommer Ravid
  • Mi Miao
  • Pierre Therizols
  • Huanhuan Gao
  • Shuliang Chen
  • Peter Novick
  • Susan Ferro-Novick
  • M Mann
  • Ane Markina-Iñarrairaegui
  • Parthasarathy Sampathkumar
  • Stephen K Burley
  • Hiro Tsuruta
  • Terence I Moy
  • Steven C Almo
  • J Michael Sauder
  • Thomas M Weiss
  • Pamela A Silver
  • Yi Shi
  • Jeffery B Bonanno
  • Anne Martel
  • Tsutomu Matsui
  • Marc Damelin
  • Lucie Sengmanivong
  • Javier Velázquez-Muriel
  • Isabelle Gagnon-Arsenault
  • Guillaume Diss
  • Jeremy Phillips
  • Jana M Mitchell
  • Catherine Dargemont
  • Rosemary Williams
  • Ruben Diaz-Avalos
  • Christian R Landry
  • Matthew D Sekedat
  • David L Stokes
  • Anna Babour
  • Josef D Franke
  • Alexandre K Dubé
  • Richard W Wozniak
  • Guillaume Charron
  • Jie Fan
  • Kristine A Willis
  • Thomas D Buford
  • R Nicholas Laribee
  • Stephen M Fuchs
  • Nowel Azzouz
  • Paolo Colombi
  • Kellie Barbara-Haley
  • Martine A Collart
  • Brian D Strahl
  • Taras Makhnevych
  • Terry Haley
  • C Patrick Lusk
  • George M Santangelo
  • Anita H Corbett
  • Yves Barral
  • T Stage-Zimmermann
  • Philipp Stelter

Detail Information

Publications42

  1. pmc Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene
    C V Heath
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Cell Biol 131:1677-97. 1995
    ..We describe here the properties of yeast strains carrying mutations in the RAT2 gene (RAT - ribonucleic acid trafficking) and the cloning of the RAT2 gene...
  2. pmc ER membrane-bending proteins are necessary for de novo nuclear pore formation
    T Renee Dawson
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Cell Biol 184:659-75. 2009
    ..We hypothesize that these ER membrane-bending proteins mediate early NPC assembly steps...
  3. pmc The structure of the scaffold nucleoporin Nup120 reveals a new and unexpected domain architecture
    Nina C Leksa
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Structure 17:1082-91. 2009
    ..We present the crystal structure of Nup120(1-757), one of the two short arms of the Y-shaped Nup84 complex...
  4. pmc Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex
    Hyuk Soo Seo
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:14281-6. 2009
    ..Here we present the crystal structure of one of its 7 components, Nup120, which reveals a beta propeller and an alpha-helical domain representing a novel fold...
  5. pmc Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes
    Benoit Palancade
    Institut Curie, Centre de Recherche, and Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique, F 75248 Paris, France
    Mol Biol Cell 18:2912-23. 2007
    ..Our results thus provide a molecular mechanism that underlies the connection between NPC and genome stability...
  6. pmc Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution
    Anne Chadrin
    Institut Jacques Monod, UMR 7592, Centre National de la Recherche Scientifique Université Paris Diderot, 75013 Paris, France
    J Cell Biol 189:795-811. 2010
    ..We hypothesize that, by modifying or stabilizing the nuclear envelope-NPC interface, Pom33 may contribute to proper distribution and/or efficient assembly of nuclear pores...
  7. pmc The integral membrane protein Pom34p functionally links nucleoporin subcomplexes
    Mi Miao
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232 8240, USA
    Genetics 172:1441-57. 2006
    ..We speculate that multiple integral membrane proteins, either within the nuclear pore domain or in the nuclear envelope, execute coordinated roles in NPC structure and function...
  8. ncbi Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p
    Huanhuan Gao
    Department of Physiology, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 278:25331-40. 2003
    ..which is too large to diffuse across the nuclear envelope, fails to concentrate in nuclei of nup133delta, rat2-1, nup85delta, nic96deltaC, and mtr7-1 cells, demonstrating that GFP-Gsp1p nuclear import is deficient...
  9. pmc Structure-function mapping of a heptameric module in the nuclear pore complex
    Javier Fernandez-Martinez
    Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY 10065, USA
    J Cell Biol 196:419-34. 2012
    ..This work demonstrates that integrative approaches based on low-resolution data of sufficient quality can generate functionally informative structures at intermediate resolution...
  10. pmc Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins
    Malik Lutzmann
    BZH Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    EMBO J 21:387-97. 2002
    ..Taken together, our data document that the Nup84p-Nup133p complex self-assembles in a modular concept from distinct smaller nucleoporin construction sets...
  11. pmc Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport
    J D Aitchison
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York 10021 6399, USA
    J Cell Biol 131:1659-75. 1995
    ..Deletion of the NUP120 gene caused clustering of NPCs at one side of the nuclear envelope, moderate nucleolar fragmentation and slower ..
  12. pmc Structure and assembly of the Nup84p complex
    S Siniossoglou
    Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 149:41-54. 2000
    ..Thus, the Nup84p complex constitutes a paradigm of an NPC structural module with distinct composition, structure, and a role in nuclear mRNA export and NPC bio- genesis...
  13. ncbi Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with nic96p
    B Kosova
    BZH, Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    J Biol Chem 275:343-50. 2000
    ..Double disruption mutants of MLP1 and MLP2 are viable and apparently not impaired in nucleocytoplasmic transport. However, overproduction of MLP1 causes nuclear accumulation of poly(A)(+) RNA in a chromatin-free area of the nucleus...
  14. pmc A novel complex of membrane proteins required for formation of a spherical nucleus
    S Siniossoglou
    BZH, Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    EMBO J 17:6449-64. 1998
    ..Thus, Spo7p and Nem1p, which exhibit a strong genetic link to nucleoporins of the Nup84p complex, fulfil an essential role in formation of a spherical nucleus and meiotic division...
  15. ncbi Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking
    E Fabre
    Institut Pasteur, Departement des Biotechnologies, Paris, France
    Annu Rev Genet 31:277-313. 1997
    ..We review here our current knowledge on the role of nucleoporins, and on the mechanism of nucleocytoplasmic transport, with emphasis on the yeast Saccharomyces cerevisiae...
  16. pmc Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins
    M T Teixeira
    Unité de Génétique Moléculaire des levures URA 1300 CNRS and UPR 927 Univ P M Curie, Institut Pasteur, Departement des Biotechnologies, Paris, France
    EMBO J 16:5086-97. 1997
    ..These data suggest that N- and C-domains of Nup145p perform independent functions, and that the in vivo cleavage observed is of functional importance...
  17. ncbi A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores
    S Siniossoglou
    European Molecular Biology Laboratory, Heidelberg Federal Republic of Germany
    Cell 84:265-75. 1996
    ..Thus, the Nup84p complex in conjunction with Sec13-type proteins is required for correct nuclear pore biogenesis...
  18. pmc Pml39, a novel protein of the nuclear periphery required for nuclear retention of improper messenger ribonucleoparticles
    Benoit Palancade
    Pores Nucléaires et Transport Nucléocytoplasmique, Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique, Paris Cedex, France
    Mol Biol Cell 16:5258-68. 2005
    ..Together, these data indicate that Pml39 is an upstream effector of the Mlps, involved in the retention of improper mRNPs in the nucleus before their export...
  19. pmc A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly
    M Bucci
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Biol Cell 9:2439-61. 1998
    ..of mutagenized cells expressing GFP-Nup49p, three complementation groups were identified: two correspond to mutant nup120 and gle2 alleles that result in clusters of NPCs...
  20. pmc The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes
    John J Scarcelli
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    J Cell Biol 178:799-812. 2007
    ..Thus, Apq12p connects nuclear pore biogenesis to the dynamics of the NE...
  21. pmc Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p
    A L Goldstein
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Mol Biol Cell 7:917-34. 1996
    ..The Rat9/Nup85 protein could not be detected in cells carrying a mutation of RAT2/NUP120, suggesting that Rat9p/Nup85p cannot be assembled into NPCs in the absence of Rat2p/Nup120p...
  22. pmc Integrative structure-function mapping of the nucleoporin Nup133 suggests a conserved mechanism for membrane anchoring of the nuclear pore complex
    Seung Joong Kim
    From the Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, California Institute for Quantitative Biosciences, Byers Hall, 1700 4th Street, Suite 503B, University of California San Francisco, San Francisco, California 94158
    Mol Cell Proteomics 13:2911-26. 2014
    ....
  23. pmc Interaction of a DNA zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory
    William H Light
    Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA
    Mol Cell 40:112-25. 2010
    ..Thus, interaction of recently repressed INO1 with the NPC alters its chromatin structure and rate of reactivation...
  24. pmc Nuclear pore complex integrity requires Lnp1, a regulator of cortical endoplasmic reticulum
    Amanda K Casey
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232
    Mol Biol Cell 26:2833-44. 2015
    ..These findings suggest that Lnp1's role in NPC integrity is separable from functions in the ER and is linked to Ndc1 and Rtn1 interactions. ..
  25. pmc Evidence for the robustness of protein complexes to inter-species hybridization
    Jean Baptiste Leducq
    Institut de Biologie Intégrative et des Systèmes, Departement de Biologie, PROTEO, Pavillon Charles Eugène Marchand, Universite Laval, Quebec City, Canada
    PLoS Genet 8:e1003161. 2012
    ....
  26. pmc Structural evolution of the membrane-coating module of the nuclear pore complex
    Xiaoping Liu
    Laboratory of Cell Biology, Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:16498-503. 2012
    ..Using recombinant nucleoporins, we show that Sp-Nup37 specifically binds the Sp-Nup120 member of the hetero-heptamer but does not bind an Sc-Nup120 homolog...
  27. pmc Ubiquitylation of the nuclear pore complex controls nuclear migration during mitosis in S. cerevisiae
    Akira Hayakawa
    Institut Jacques Monod, University Paris Diderot, Sorbonne Paris Cité, Unite Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Cedex 13, 75205 Paris, France
    J Cell Biol 196:19-27. 2012
    ..This led to defects in nuclear segregation at the onset of mitosis. Thus, defining ubiquitylation of the yeast NPC highlights yet-unexplored functions of this essential organelle in cell division...
  28. pmc The nuclear pore complex mediates binding of the Mig1 repressor to target promoters
    Nayan J Sarma
    Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, Mississippi, United States of America
    PLoS ONE 6:e27117. 2011
    ..Surprisingly, despite its abundant presence in the nucleus of glucose-grown nup120Δ or nup133Δ cells, Mig1 has lost its ability to interact with target promoters...
  29. pmc A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae
    Svetlana Dokudovskaya
    Institut Jacques Monod, CNRS UMR 7592, Batiment Buffon, Paris, France
    Mol Cell Proteomics 10:M110.006478. 2011
    ..These data demonstrate that the SEA complex is an additional member of a family of membrane coating and vesicle tethering assemblies, extending the repertoire of protocoatomer-related complexes...
  30. pmc Factors affecting nuclear export of the 60S ribosomal subunit in vivo
    T Stage-Zimmermann
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Mol Biol Cell 11:3777-89. 2000
    ..Together, these data further define the requirements for ribosomal subunit export and suggest a biological function for KAP120...
  31. ncbi Requirements for the nuclear export of the small ribosomal subunit
    Terence I Moy
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA
    J Cell Sci 115:2985-95. 2002
    ..Together with further analysis of existing mutants, we show that nucleoporins previously shown to be defective in ribosomal assembly are also defective in export of the small ribosomal subunit...
  32. pmc In situ analysis of spatial relationships between proteins of the nuclear pore complex
    Marc Damelin
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and the Dana Farber Cancer Institute, 1 Jimmy Fund Way, Boston, MA 02115, USA
    Biophys J 83:3626-36. 2002
    ..Independent experiments are consistent with the FRET results: Nup120 localization is perturbed in the nic96-1 mutant, as is Nup82 localization in the nup116Delta mutant...
  33. pmc The Ccr4-Not complex interacts with the mRNA export machinery
    Shana C Kerr
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, United States of America
    PLoS ONE 6:e18302. 2011
    ..However, the functional significance of this regulation is poorly understood...
  34. pmc General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants
    Rune Thomsen
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, University of Aarhus, DK 8000 Aarhus C, Denmark
    RNA 14:706-16. 2008
    ..Consistent with a causal role of excess nuclear mRNA, nucleolar morphology in an mRNA export mutant environment remains intact when transcription by RNA polymerase II is inhibited...
  35. pmc Lumenal interactions in nuclear pore complex assembly and stability
    William T Yewdell
    Howard Hughes Medical Institute, New York, NY 10065, USA
    Mol Biol Cell 22:1375-88. 2011
    ..These findings support a role for Heh1p in the assembly or stability of the NPC, potentially through the formation of a lumenal bridge with Pom152p...
  36. pmc Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region
    Pierre Therizols
    Unité de Génétique Moléculaire des Levures URA 2171 Centre National de la Recherche Scientifique, UFR 927 Université Pierre et Marie Curie, Département Structure et Dynamique des Génomes, Institut Pasteur, 75724 Paris Cedex, France
    J Cell Biol 172:189-99. 2006
    ..They also indicate that anchoring of telomeres is essential for efficient repair of DSBs occurring therein and is important for preserving genome integrity...
  37. doi Precise mapping of subunits in multiprotein complexes by a versatile electron microscopy label
    Dirk Flemming
    Biochemie Zentrum der Universitat Heidelberg, Heidelberg, Germany
    Nat Struct Mol Biol 17:775-8. 2010
    ..This tag is readily visible on single particles and becomes exceptionally distinct after image processing and classification. Thus, our method is applicable for the exact topological mapping of subunits in macromolecular complexes...
  38. pmc Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways
    Tommer Ravid
    Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    EMBO J 25:533-43. 2006
    ..Thus, while Doa10 ubiquitinates both membrane and soluble proteins, the mechanisms of subsequent proteasome targeting differ...
  39. pmc A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export
    Wei Yao
    Biochemie Zentrum der Universitat Heidelberg, Heidelberg, Germany
    EMBO J 27:6-16. 2008
    ..Thus, the Mex67-Mtr2 export receptor employs a versatile binding platform on its surface that could create a crosstalk between mRNA and ribosome export pathways...
  40. pmc Arx1 is a nuclear export receptor for the 60S ribosomal subunit in yeast
    Nai Jung Hung
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Biol Cell 19:735-44. 2008
    ..These results show that Arx1 can directly bridge the interaction between the pre-60S particle and the NPC and thus is a third export receptor for the 60S subunit in yeast...
  41. pmc Yeast screens identify the RNA polymerase II CTD and SPT5 as relevant targets of BRCA1 interaction
    Craig B Bennett
    Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America
    PLoS ONE 3:e1448. 2008
    ..elongation (SPT4, SPT5, CTK1, DEF1) to nucleopore-mediated mRNA export (ASM4, MLP1, MLP2, NUP2, NUP53, NUP120, NUP133, NUP170, NUP188, POM34) and cytoplasmic mRNA decay at P-bodies (CCR4, DHH1)...
  42. pmc The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
    Cristina González-Aguilera
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla CSIC, 41092 Sevilla, Spain
    Mol Biol Cell 19:4310-8. 2008
    ....