GPA2

Summary

Gene Symbol: GPA2
Description: guanine nucleotide-binding protein subunit alpha
Alias: SSP101
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. ncbi Gpa2p, a G-protein alpha-subunit, regulates growth and pseudohyphal development in Saccharomyces cerevisiae via a cAMP-dependent mechanism
    E Kübler
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, D 37077 Gottingen, Germany
    J Biol Chem 272:20321-3. 1997
  2. pmc Yeast pseudohyphal growth is regulated by GPA2, a G protein alpha homolog
    M C Lorenz
    Department of Genetics, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 16:7008-18. 1997
  3. ncbi G-protein coupled receptor from yeast Saccharomyces cerevisiae
    C W Yun
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan
    Biochem Biophys Res Commun 240:287-92. 1997
  4. ncbi The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast
    Corinne E Zeller
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:25168-76. 2007
  5. ncbi Kelch repeat protein interacts with the yeast Galpha subunit Gpa2p at a site that couples receptor binding to guanine nucleotide exchange
    Thiruvur Niranjan
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 282:24231-8. 2007
  6. pmc Involvement of distinct G-proteins, Gpa2 and Ras, in glucose- and intracellular acidification-induced cAMP signalling in the yeast Saccharomyces cerevisiae
    S Colombo
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Flanders, Belgium
    EMBO J 17:3326-41. 1998
  7. pmc Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast
    Tom Peeters
    Laboratory of Molecular Cell Biology, Katholieke Universiteit Leuven, and Department of Molecular Microbiology, Flanders Interuniversity Institute of Biotechnology, Kasteelpark Arenberg 31, B 3001 Leuven Heverlee, Belgium
    Proc Natl Acad Sci U S A 103:13034-9. 2006
  8. ncbi A Saccharomyces cerevisiae G-protein coupled receptor, Gpr1, is specifically required for glucose activation of the cAMP pathway during the transition to growth on glucose
    L Kraakman
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Institute of Botany and Microbiology
    Mol Microbiol 32:1002-12. 1999
  9. pmc Galpha subunit Gpa2 recruits kelch repeat subunits that inhibit receptor-G protein coupling during cAMP-induced dimorphic transitions in Saccharomyces cerevisiae
    Toshiaki Harashima
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 16:4557-71. 2005
  10. pmc Sir2-independent life span extension by calorie restriction in yeast
    Matt Kaeberlein
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    PLoS Biol 2:E296. 2004

Scientific Experts

  • Igor L Medintz
  • Mikael Molin
  • Toshiaki Harashima
  • Matt Kaeberlein
  • Brian K Kennedy
  • M C Lorenz
  • Ailan Lu
  • Jeanne P Hirsch
  • D Grant Willhite
  • Elizabeth X Kwan
  • Amit R Reddi
  • Joseph Heitman
  • Roli Budhwar
  • Revathi S Iyer
  • Volodymyr Y Nazarko
  • J M Thevelein
  • Johan M Thevelein
  • Corinne E Zeller
  • J Heitman
  • Thiruvur Niranjan
  • Tom Peeters
  • C W Yun
  • Kathryn T Kirkland
  • Stanley Fields
  • Michael Rutzler
  • X Pan
  • Ying Wang
  • J Winderickx
  • Montserrat Batlle
  • Y Xue
  • Vera A Cherkasova
  • J H de Winde
  • P Ma
  • S Colombo
  • Stephen E Wright
  • Jennifer R Brigati
  • Katie E Selcer
  • Zachary A Duck
  • E Lenssen
  • Joshua E Denny
  • M K Raghuraman
  • Gina M Alvino
  • Bonita J Brewer
  • Leonid Kruglyak
  • Antonio Bedalov
  • Eric J Foss
  • Valeria C Culotta
  • Scott Tsuchiyama
  • M A Souza
  • P Van Dijck
  • J P Hirsch
  • L Kraakman
  • M Versele
  • M Vanhalewyn
  • K Ansari
  • Maitreyi Das
  • M Donzeau
  • Andriy A Sibirny
  • Paike Jayadeva Bhat
  • Xuedong Guo
  • Henrik G Dohlman
  • Stephen C Parnell
  • Jacob Victor
  • Matthias Versele
  • David Nauwelaers
  • Wendy Louwet
  • Scott Anderson
  • Ruud Geladé
  • John R Yates
  • K Yamamoto
  • H Tamaki
  • H Kumagai
  • R Nakayama
  • Andre Reissaus
  • Saeed Tavazoie
  • C Gökçe Güldal
  • Xiuying Zhang
  • Wolfhard Bandlow
  • E Kübler
  • Lisa Schneper
  • Magdalena Budzowska
  • James R Broach
  • Michael Pierce
  • Yong Xue
  • Elaine A Elion
  • Ryan McCully
  • Yunmei Wang
  • Alan Hinnebusch
  • David A Green
  • C De Virgilio

Detail Information

Publications37

  1. ncbi Gpa2p, a G-protein alpha-subunit, regulates growth and pseudohyphal development in Saccharomyces cerevisiae via a cAMP-dependent mechanism
    E Kübler
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, D 37077 Gottingen, Germany
    J Biol Chem 272:20321-3. 1997
    ..Of the two yeast G-protein alpha-subunits (GPA1 and GPA2), only GPA1 has been well studied and shown to negatively regulate the mitogen-activated protein kinase pathway ..
  2. pmc Yeast pseudohyphal growth is regulated by GPA2, a G protein alpha homolog
    M C Lorenz
    Department of Genetics, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 16:7008-18. 1997
    ..The mechanisms by which nitrogen limitation regulates this process are currently unknown. We have found that GPA2, one of the two heterotrimeric G protein alpha subunit homologs in yeast, regulates pseudohyphal differentiation...
  3. ncbi G-protein coupled receptor from yeast Saccharomyces cerevisiae
    C W Yun
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan
    Biochem Biophys Res Commun 240:287-92. 1997
    ..It is likely that a Gpr1p monitors the extracellular signal such as nutrition and transduce it via Gpa2p a possible positive regulator of cAMP level...
  4. ncbi The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast
    Corinne E Zeller
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:25168-76. 2007
    ..In the yeast Saccharomyces cerevisiae there are two known Galpha proteins (Gpa1 and Gpa2) but only one Gbetagamma, which binds only to Gpa1...
  5. ncbi Kelch repeat protein interacts with the yeast Galpha subunit Gpa2p at a site that couples receptor binding to guanine nucleotide exchange
    Thiruvur Niranjan
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 282:24231-8. 2007
    ..The results suggest that binding of Gpa2p to Krh1p does not resemble the binding of Galpha subunits to either Gbeta subunits or effectors, but it instead represents a novel type of functional interaction...
  6. pmc Involvement of distinct G-proteins, Gpa2 and Ras, in glucose- and intracellular acidification-induced cAMP signalling in the yeast Saccharomyces cerevisiae
    S Colombo
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Flanders, Belgium
    EMBO J 17:3326-41. 1998
    ..Stimulation by glucose was dependent on the Galpha-protein Gpa2, whose deletion confers the typical phenotype associated with a reduced cAMP level: higher heat resistance, a ..
  7. pmc Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast
    Tom Peeters
    Laboratory of Molecular Cell Biology, Katholieke Universiteit Leuven, and Department of Molecular Microbiology, Flanders Interuniversity Institute of Biotechnology, Kasteelpark Arenberg 31, B 3001 Leuven Heverlee, Belgium
    Proc Natl Acad Sci U S A 103:13034-9. 2006
    ..domain receptor, Gpr1, that was proposed to activate adenylate cyclase through the G(alpha) protein Gpa2. Consistently, we show here that adenylate cyclase binds only to active, GTP-bound Gpa2...
  8. ncbi A Saccharomyces cerevisiae G-protein coupled receptor, Gpr1, is specifically required for glucose activation of the cAMP pathway during the transition to growth on glucose
    L Kraakman
    Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Institute of Botany and Microbiology
    Mol Microbiol 32:1002-12. 1999
    ..This glucose-induced cAMP signal is dependent on the G alpha-protein Gpa2. We show that the G-protein coupled receptor (GPCR) Gpr1 interacts with Gpa2 and is required for stimulation of ..
  9. pmc Galpha subunit Gpa2 recruits kelch repeat subunits that inhibit receptor-G protein coupling during cAMP-induced dimorphic transitions in Saccharomyces cerevisiae
    Toshiaki Harashima
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 16:4557-71. 2005
    ..The Saccharomyces cerevisiae GPCR Gpr1 and associated Galpha subunit Gpa2 sense extracellular carbon sources (including glucose) to govern filamentous growth...
  10. pmc Sir2-independent life span extension by calorie restriction in yeast
    Matt Kaeberlein
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    PLoS Biol 2:E296. 2004
    ..These findings indicate that Sir2 and calorie restriction act in parallel pathways to promote longevity in yeast and, perhaps, higher eukaryotes...
  11. ncbi The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits
    Toshiaki Harashima
    Department of Molecular Genetics and Microbiology, Durham, North Carolina 27710, USA
    Mol Cell 10:163-73. 2002
    ..Saccharomyces cerevisiae GPCR Gpr1 senses glucose and controls filamentous growth via an unusual Galpha protein, Gpa2, which lacks any known Gbetagamma subunits...
  12. pmc The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae
    M C Lorenz
    Departments of Genetics, Pharmacology and Cancer Biology, Microbiology, and Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genetics 154:609-22. 2000
    ..Recent studies have established a physical and functional link between the Galpha protein Gpa2 and the G protein-coupled receptor homolog Gpr1...
  13. ncbi Krh1p and Krh2p act downstream of the Gpa2p G(alpha) subunit to negatively regulate haploid invasive growth
    Montserrat Batlle
    Brookdale Department of Molecular, Cell, and Developmental Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Cell Sci 116:701-10. 2003
    ..A two-hybrid screen using a constitutively active allele of GPA2 identified the KRH1 gene as encoding a potential binding partner of Gpa2p...
  14. ncbi The kelch proteins Gpb1 and Gpb2 inhibit Ras activity via association with the yeast RasGAP neurofibromin homologs Ira1 and Ira2
    Toshiaki Harashima
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell 22:819-30. 2006
    The G protein-coupled receptor Gpr1 and associated Galpha subunit Gpa2 govern dimorphic transitions in response to extracellular nutrients by signaling coordinately with Ras to activate adenylyl cyclase in the yeast Saccharomyces ..
  15. pmc Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein: studies on its structure and possible functions
    M Nakafuku
    Institute of Medical Science, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 85:1374-8. 1988
    ..Here we report the isolation of another G-protein-homologous gene, GPA2, which encodes an amino acid sequence of 449 amino acid residues with a Mr of 50,516...
  16. pmc GPR1 encodes a putative G protein-coupled receptor that associates with the Gpa2p Galpha subunit and functions in a Ras-independent pathway
    Y Xue
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, NY 10029, USA
    EMBO J 17:1996-2007. 1998
    ..growth control also involves a signal mediated by the heterotrimeric G protein alpha subunit homolog encoded by GPA2. A GPA2 null allele conferred a severe growth defect on cells containing a null allele of RAS2, although either ..
  17. doi Pheromone responsiveness is regulated by components of the Gpr1p-mediated glucose sensing pathway in Saccharomyces cerevisiae
    D Grant Willhite
    Department of Biology, Tennessee Weslyan College, TN, USA
    Yeast 31:361-74. 2014
    ..These data suggest that activity of the GPCR-mediated mating pathway in S. cerevisiae is modulated by extracellular glucose concentrations through the only other GPCR in MATa cells, Gpr1p...
  18. pmc A natural polymorphism in rDNA replication origins links origin activation with calorie restriction and lifespan
    Elizabeth X Kwan
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS Genet 9:e1003329. 2013
    ....
  19. pmc SOD1 integrates signals from oxygen and glucose to repress respiration
    Amit R Reddi
    Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA
    Cell 152:224-35. 2013
    ..Our data therefore may provide mechanistic insight into how rapidly proliferating cells and many cancers accomplish glucose-mediated repression of respiration in favor of aerobic glycolysis...
  20. ncbi Gpr1p, a putative G-protein coupled receptor, regulates glucose-dependent cellular cAMP level in yeast Saccharomyces cerevisiae
    C W Yun
    Graduate School of Agriculture, Kyoto University, Kyoto, 606 8502, Japan
    Biochem Biophys Res Commun 252:29-33. 1998
    ..The glucose-induced higher cAMP level found in the strain with GPA2 in multicopy plasmid decreased by deletion of GPR1 gene...
  21. doi Life span extension and H(2)O(2) resistance elicited by caloric restriction require the peroxiredoxin Tsa1 in Saccharomyces cerevisiae
    Mikael Molin
    CMB, University of Gothenburg, Medicinaregatan 9C, S 413 90 Göteborg, Sweden
    Mol Cell 43:823-33. 2011
    ..The data demonstrate that Tsa1 is required to ensure yeast longevity and that CR extends yeast life span, in part, by counteracting age-induced hyperoxidation of this peroxiredoxin...
  22. pmc Nutrient control of yeast PKA activity involves opposing effects on phosphorylation of the Bcy1 regulatory subunit
    Roli Budhwar
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Biol Cell 21:3749-58. 2010
    ..Stimulation of Bcy1 phosphorylation by Gpb1 and Gpb2 produces a form of Bcy1 that is more stable and is a more effective PKA inhibitor...
  23. doi Pseudohyphal differentiation defect due to mutations in GPCR and ammonium signaling is suppressed by low glucose concentration: a possible integrated role for carbon and nitrogen limitation
    Revathi S Iyer
    Laboratory of Molecular Genetics, School of Biosciences and Bioengineering, Indian Institute of Technology, Mumbai 400 076, India
    Curr Genet 54:71-81. 2008
    ..Further, the pseudohyphal defect of gpa2, gpr1and gpa2gpr1 but not the mep2 mutant strain is overcome on SLALD...
  24. ncbi G-protein-coupled receptor Gpr1 and G-protein Gpa2 of cAMP-dependent signaling pathway are involved in glucose-induced pexophagy in the yeast Saccharomyces cerevisiae
    Volodymyr Y Nazarko
    Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14 16, Lviv 79005, Ukraine
    Cell Biol Int 32:502-4. 2008
    ..We have found that in Saccharomyces cerevisiae, defect of G-protein-coupled receptor Gpr1 and G-protein Gpa2, both the components of cAMP-signaling pathway, strongly suppressed glucose-induced degradation of matrix ..
  25. ncbi Transcript and proteomic analyses of wild-type and gpa2 mutant Saccharomyces cerevisiae strains suggest a role for glycolytic carbon source sensing in pseudohyphal differentiation
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Mol Biosyst 3:623-34. 2007
    ..monosaccharide glucose, has been shown to induce a hyper-filamentous growth phenotype in a strain deficient for GPA2 which codes for a Galpha protein component that interacts with the glucose-sensing receptor Gpr1p to regulate ..
  26. pmc The MEP2 ammonium permease regulates pseudohyphal differentiation in Saccharomyces cerevisiae
    M C Lorenz
    Departments of Genetics, Duke University Medical Center, 322 CARL Building, Research Drive, Durham, NC 27710, USA
    EMBO J 17:1236-47. 1998
    ..This dimorphic transition is regulated by the Galpha protein GPA2, by RAS2, and by elements of the pheromone-responsive MAP kinase cascade, yet the mechanisms by which nitrogen ..
  27. ncbi New aspects of the glucose activation of the H(+)-ATPase in the yeast Saccharomyces cerevisiae
    M A Souza
    Laboratório de Bioquímica e Fisiologia de Microrganismos, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro 35 400 000 Ouro Preto, MG, Brazil
    Microbiology 147:2849-55. 2001
    ..Moreover, the involvement of protein kinase C in the regulation of ATPase activity is confirmed. Finally, a model pathway is presented for sensing and transmission of the glucose activation signal of the yeast H(+)-ATPase...
  28. pmc Cyclic AMP-dependent protein kinase regulates pseudohyphal differentiation in Saccharomyces cerevisiae
    X Pan
    Departments of Genetics, Pharmacology and Cancer Biology, Microbiology, and Medicine, The Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 19:4874-87. 1999
    ..protein (MAP) kinase cascade and a second signaling cascade involving the receptor Gpr1, the Galpha protein Gpa2, Ras2, and cyclic AMP (cAMP)...
  29. ncbi A mutation in Saccharomyces cerevisiae adenylate cyclase, Cyr1K1876M, specifically affects glucose- and acidification-induced cAMP signalling and not the basal cAMP level
    M Vanhalewyn
    Laboratorium voor Moleculaire Celbiologie, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, Flanders, Belgium
    Mol Microbiol 33:363-76. 1999
    ..These results demonstrate the usefulness of the cyr1met1876 (lcr1) mutation for epistasis studies on the signalling function of the cAMP pathway...
  30. ncbi Genes determining yeast replicative life span in a long-lived genetic background
    Matt Kaeberlein
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Mech Ageing Dev 126:491-504. 2005
    ..previously reported to increase yeast life span, we find that deletion of FOB1, deletion of SCH9, and deletion of GPA2, GPR1, or HXK2 (three genetic models of calorie restriction) significantly enhanced longevity...
  31. pmc The yeast trimeric guanine nucleotide-binding protein alpha subunit, Gpa2p, controls the meiosis-specific kinase Ime2p activity in response to nutrients
    M Donzeau
    Institut fur Genetik und Mikrobiologie, Ludwig Maximilians Universitat Munchen, D 80638 Munich, Germany
    Mol Cell Biol 19:6110-9. 1999
    ..Furthermore, deletion of GPA2 accelerates sporulation on low-nitrogen medium. These observations are consistent with the following model...
  32. pmc Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast
    Ying Wang
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
    PLoS Biol 2:E128. 2004
    ..We have used global analysis of gene expression to assess the roles of the small GTP-binding proteins, Ras2 and Gpa2, in mediating the transcriptional response to glucose...
  33. ncbi SUT2 is a novel multicopy suppressor of low activity of the cAMP/protein kinase A pathway in yeast
    Michael Rutzler
    Ludwig Maximilians Universitat Munchen, Department Biologie I, Bereich Genetik, Munich, Germany
    Eur J Biochem 271:1284-91. 2004
    ....
  34. ncbi A novel functional link between MAP kinase cascades and the Ras/cAMP pathway that regulates survival
    Vera A Cherkasova
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Curr Biol 13:1220-6. 2003
    ..The conserved RasGEF Cdc25 is a likely control point, because Kss1 and Fus3 complexes associate with and phosphorylate Cdc25. Cross-regulation of Cdc25 may be a general way that MAPKs control Ras signaling networks...
  35. ncbi Phospholipase C binds to the receptor-like GPR1 protein and controls pseudohyphal differentiation in Saccharomyces cerevisiae
    K Ansari
    Max Planck Institut fur experimentelle Medizin, Hermann Rein Strasse 3, D 37075 Gottingen, Germany
    J Biol Chem 274:30052-8. 1999
    ..Plc1p binds to Gpr1p in either the presence or absence of Gpa2, whereas the Gpr1p/Gpa2p association depends on the presence of Plc1p...
  36. pmc A novel regulator of G protein signalling in yeast, Rgs2, downregulates glucose-activation of the cAMP pathway through direct inhibition of Gpa2
    M Versele
    Laboratorium voor Moleculaire Celbiologie, Instituut voor Plantkunde en Microbiologie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Leuven Heverlee, Flanders, Belgium
    EMBO J 18:5577-91. 1999
    ..as a negative regulator of glucose-induced cAMP signalling through direct GTPase activation of the Gs-alpha protein Gpa2. Rgs2 and Gpa2 constitute the second cognate RGS-G-alpha protein pair identified in yeast, in addition to the ..
  37. ncbi Saccharomyces cerevisiae Ccr4-not complex contributes to the control of Msn2p-dependent transcription by the Ras/cAMP pathway
    E Lenssen
    Department Biochimie Medicale, CMU, 1 rue Michel Servet, 1211 Geneve 4, Switzerland CEA Saclay, France
    Mol Microbiol 43:1023-37. 2002
    ..Together, our results suggest that the Ccr4-Not complex may function as an effector of the Ras/cAMP pathway that contributes to repress basal, stress- and starvation-induced transcription by Msn2/4p...