DBF2

Summary

Gene Symbol: DBF2
Description: serine/threonine-protein kinase DBF2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis
    Dane A Mohl
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    J Cell Biol 184:527-39. 2009
  2. pmc Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex
    Wenying Shou
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    BMC Mol Biol 3:3. 2002
  3. pmc A defect of Kap104 alleviates the requirement of mitotic exit network gene functions in Saccharomyces cerevisiae
    Kazuhide Asakawa
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Genetics 162:1545-56. 2002
  4. pmc Regulation of the mitotic exit protein kinases Cdc15 and Dbf2
    R Visintin
    Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17 233, 40 Ames Street, Cambridge MA 02139, USA
    Mol Biol Cell 12:2961-74. 2001
  5. ncbi Regulation of the localization of Dbf2 and mob1 during cell division of saccharomyces cerevisiae
    S Yoshida
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Japan
    Genes Genet Syst 76:141-7. 2001
  6. pmc Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex
    A S Mah
    Division of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 98:7325-30. 2001
  7. ncbi Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling
    Yanchang Wang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 112:697-709. 2003
  8. ncbi The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis
    L M Frenz
    Division of Yeast Genetics, National Institute for Medical Research, Medical Research Council, The Ridgeway, Mill Hill, London NW7 1AA, UK
    J Cell Sci 113:3399-408. 2000
  9. ncbi Cdc14 activates cdc15 to promote mitotic exit in budding yeast
    S L Jaspersen
    Department of Physiology, University of California, San Francisco, CA 94143 0444, USA
    Curr Biol 10:615-8. 2000
  10. pmc Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia Unit, DKFZ ZMBH Alliance, German Cancer Research Center, 69120 Heidelberg, Germany
    Genes Dev 25:875-88. 2011

Scientific Experts

  • Wenying Shou
  • Marco Geymonat
  • Dane A Mohl
  • Franz Meitinger
  • L H Johnston
  • Gislene Pereira
  • Birgit Hub
  • J H Toyn
  • R Visintin
  • Jose Antonio Rodriguez-Rodriguez
  • H Y Liu
  • Christopher M Yellman
  • Hauke Drechsler
  • Dana M Alessi Wolken
  • C L Denis
  • Michelle A Attner
  • Manuel Hotz
  • Younghoon Oh
  • Hanswalter Zentgraf
  • J Chen
  • Ying Chou Chen
  • Angelika Amon
  • Cornelia König
  • Valentina Rossio
  • Y C Chiang
  • Rami Rahal
  • S L Jaspersen
  • Vasso Makrantoni
  • N Grandin
  • Mark Corbett
  • L M Frenz
  • Jan Stoepel
  • A Amon
  • Hong Hwa Lim
  • Ethel Queralt
  • Soraya Játiva
  • Yanchang Wang
  • Yolanda Moyano
  • Ann Na Tan
  • Dimitris Liakopoulos
  • A L Johnson
  • G Shirleen Roeder
  • Joseph McInnes
  • Liza A Pon
  • Kazuhide Asakawa
  • Saravanan Palani
  • Thomas Wegleiter
  • Kuang Jung Chang
  • Erfei Bi
  • Derek Lindstrom
  • R Shah
  • Christian Leisner
  • Cristina Manatschal
  • Yves Barral
  • Carsten Wloka
  • Daici Chen
  • Jackie Vogel
  • Peter Orlean
  • Dan E Gottschling
  • S Yoshida
  • Raymond Deshaies
  • A S Mah
  • Jimmy Ouellet
  • Wolf D Lehmann
  • Astrid Hofmann
  • Martin E Boehm
  • Elmar Schiebel
  • Elena Galati
  • Michael Weinreich
  • S Irniger
  • Matteo Ferrari
  • Katsuhiko Shirahige
  • Simonetta Piatti
  • Takashi Sutani
  • Giovanna Lucchini
  • Boryana Petrova
  • Ilde Mancini Lombardi
  • Daniela Trinca Bertazzi
  • Achille Pellicioli
  • Hiromi Maekawa
  • J Rappsilber
  • M Mann
  • D C Audino
  • V Badarinarayana
  • D O Morgan
  • E S Hwang
  • Paul Dennison
  • M Charbonneau
  • Michael J R Stark
  • Peter J Coote

Detail Information

Publications52

  1. pmc Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis
    Dane A Mohl
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    J Cell Biol 184:527-39. 2009
    ..In this study, we show that a MEN component, protein kinase Dbf2-Mob1, promotes transfer of Cdc14 to the cytoplasm and consequent exit from mitosis by direct phosphorylation of ..
  2. pmc Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex
    Wenying Shou
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    BMC Mol Biol 3:3. 2002
    ..In pre-anaphase cells, Cdc14 is sequestered to the nucleolus by Net1 as a part of the RENT complex. When cells are primed to exit mitosis, the RENT complex is disassembled and Cdc14 is released from the nucleolus...
  3. pmc A defect of Kap104 alleviates the requirement of mitotic exit network gene functions in Saccharomyces cerevisiae
    Kazuhide Asakawa
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Genetics 162:1545-56. 2002
    ..These results suggest that a mutation in Kap104 stimulates exit from mitosis through the activation of Cdc14 and implies a novel role for Kap104 in cell-cycle progression in budding yeast...
  4. pmc Regulation of the mitotic exit protein kinases Cdc15 and Dbf2
    R Visintin
    Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17 233, 40 Ames Street, Cambridge MA 02139, USA
    Mol Biol Cell 12:2961-74. 2001
    ..factor Lte1, the GTPase activating protein complex Bub2-Bfa1/Byr4, and several protein kinases including Cdc15 and Dbf2. Here we investigate the regulation of the protein kinases Dbf2 and Cdc15...
  5. ncbi Regulation of the localization of Dbf2 and mob1 during cell division of saccharomyces cerevisiae
    S Yoshida
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Japan
    Genes Genet Syst 76:141-7. 2001
    ..yeast, MEN consists of the protein phosphatase Cdc14, the ras-like GTPase Tem1, protein kinases Cdc15, Cdc5, Dbf2 and Dbf2-binding protein Mob1...
  6. pmc Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex
    A S Mah
    Division of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 98:7325-30. 2001
    ..turn, is regulated by a group of proteins, the mitotic exit network (MEN), which includes Lte1, Tem1, Cdc5, Cdc15, Dbf2/Dbf20, and Mob1. The direct biochemical interactions between the components of the MEN remain largely unresolved...
  7. ncbi Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling
    Yanchang Wang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 112:697-709. 2003
    ..Thus, Amn1 is part of a daughter-specific switch that helps cells exit from mitotic exit and reset the cell cycle...
  8. ncbi The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis
    L M Frenz
    Division of Yeast Genetics, National Institute for Medical Research, Medical Research Council, The Ridgeway, Mill Hill, London NW7 1AA, UK
    J Cell Sci 113:3399-408. 2000
    b>Dbf2 is a multifunctional protein kinase in Saccharomyces cerevisiae that functions in transcription, the stress response and as part of a network of genes in exit from mitosis...
  9. ncbi Cdc14 activates cdc15 to promote mitotic exit in budding yeast
    S L Jaspersen
    Department of Physiology, University of California, San Francisco, CA 94143 0444, USA
    Curr Biol 10:615-8. 2000
    ..Instead, Cdc15 dephosphorylation may promote some additional function of Cdc15 that is independent of its effects on Cdc14 activation...
  10. pmc Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia Unit, DKFZ ZMBH Alliance, German Cancer Research Center, 69120 Heidelberg, Germany
    Genes Dev 25:875-88. 2011
    ..Here we identified the conserved F-BAR protein Hof1 as a substrate of the MEN kinase complex Dbf2-Mob1 during cytokinesis...
  11. ncbi Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus
    R Visintin
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    Nature 398:818-23. 1999
    ..A highly conserved signalling cascade, critical for the exit from mitosis, is required for this movement of Cdc14 during anaphase. Furthermore, we have identified a negative regulator of Cdc14, Cfi1, that anchors Cdc14 in the nucleolus...
  12. pmc Inactivation of mitotic kinase triggers translocation of MEN components to mother-daughter neck in yeast
    Hong Hwa Lim
    Institute of Molecular and Cell Biology, Singapore 117609
    Mol Biol Cell 14:4734-43. 2003
    ..is regulated by the mitotic exit network (MEN), which includes a GTPase (Tem1) and various kinases (Cdc15, Cdc5, Dbf2, and Dbf20). Here, we show that Dbf2 and Dbf20 functions are necessary for the execution of cytokinesis...
  13. pmc A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae
    S L Jaspersen
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    Mol Biol Cell 9:2803-17. 1998
    ..yeast Saccharomyces cerevisiae, members of a large class of late mitotic mutants, including cdc15, cdc5, cdc14, dbf2, and tem1, arrest in anaphase with a phenotype similar to that of cells expressing nondegradable forms of mitotic ..
  14. pmc Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast
    Younghoon Oh
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Biol Cell 23:2445-56. 2012
    ..Here, we report that Dbf2, a conserved mitotic exit kinase, localizes to the division site after Chs2 and directly phosphorylates Chs2 on ..
  15. pmc The mitotic exit network Mob1p-Dbf2p kinase complex localizes to the nucleus and regulates passenger protein localization
    Jan Stoepel
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Biol Cell 16:5465-79. 2005
    ..These findings reveal kinetochores as sites for MEN signaling and implicate MEN in coordinating chromosome segregation and/or spindle integrity with mitotic exit and cytokinesis via regulation of chromosome passenger proteins...
  16. pmc Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia Unit, DKFZ ZMBH Alliance, 69120 Heidelberg, Germany
    Mol Biol Cell 24:1290-304. 2013
    The conserved NDR-kinase Dbf2 plays a critical role in cytokinesis in budding yeast. Among its cytokinesis-related substrates is the F-BAR protein Hof1...
  17. ncbi IQGAP and mitotic exit network (MEN) proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae
    Mark Corbett
    Biological Sciences, Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK
    Eur J Cell Biol 85:1201-15. 2006
    ..These data establish a mechanistic dependency of cell separation upon an intermediate step requiring actomyosin ring assembly...
  18. pmc The Swi5 transcription factor of Saccharomyces cerevisiae has a role in exit from mitosis through induction of the cdk-inhibitor Sic1 in telophase
    J H Toyn
    Division of Yeast Genetics, National Institute for Medical Research, London, United Kingdom
    Genetics 145:85-96. 1997
    ..Second, both are hypersensitive to overexpression of the B cyclin CLB2. Thus Swi5-dependent activation of the SIC1 gene contributes to the deactivation of the B cyclin kinase, and hence exit from mitosis...
  19. doi Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast
    Franz Meitinger
    German Cancer Research Centre, DKFZ ZMBH Alliance, Molecular Biology of Centrosomes and Cilia Unit, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany
    J Cell Sci 123:1851-61. 2010
    ..This defect was partially suppressed by increased levels of Cyk3. We therefore propose that the MEN directly controls cytokinesis via targeting of Inn1, Cyk3 and Chs2 to the bud neck...
  20. pmc The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14
    Rami Rahal
    David H Koch Institute for Integrative Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Cell Cycle 7:3262-72. 2008
    ..Finally, we present evidence that the Cdc5-Cdc14 association is direct, further supporting the central role of Cdc5 in Cdc14 localization...
  21. pmc The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition
    J H Toyn
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    EMBO J 13:1103-13. 1994
    Thermosensitive mutations in the DBF2 gene arrest the cell cycle during nuclear division...
  22. ncbi P40SDB25, a putative CDK inhibitor, has a role in the M/G1 transition in Saccharomyces cerevisiae
    J D Donovan
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    Genes Dev 8:1640-53. 1994
    The Saccharomyces cerevisiae protein kinase Dbf2 carries out an essential function in late mitosis, and its kinase activity is cell-cycle regulated around anaphase/telophase...
  23. pmc The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase
    M Shirayama
    Department of Biology, University of Tokyo, Japan
    Mol Cell Biol 14:7476-82. 1994
    ..The genetic interaction among LTE1, TEM1, and CDC15 indicates that they cooperatively play an essential role for termination of M phase...
  24. pmc The product of the Saccharomyces cerevisiae cell cycle gene DBF2 has homology with protein kinases and is periodically expressed in the cell cycle
    L H Johnston
    Laboratory of Cell Propagation, National Institute for Medical Research, Mill Hill, London
    Mol Cell Biol 10:1358-66. 1990
    ..In this paper, one of them, dbf2, is characterized in detail...
  25. ncbi The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk-dependent phosphorylation
    R Visintin
    Whitehead Institute for Biomedical Research, Cambridge Center, Massachusetts 02142, USA
    Mol Cell 2:709-18. 1998
    ..Feedback between these pathways may lead to precipitous collapse of mitotic CDK activity and help coordinate exit from mitosis...
  26. ncbi Requirement of Saccharomyces cerevisiae Ras for completion of mitosis
    T Morishita
    Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, Tokyo, Japan
    Science 270:1213-5. 1995
    ..The lethality of the triple disruption was suppressed by the multicopies of CDC5, CDC15, DBF2, SPO12, and TEM1, all of which function in the completion of the M phase...
  27. pmc Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae
    Wenying Shou
    Division of Biology, 156 29 Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    BMC Genet 3:4. 2002
    ..However, the remaining tab mutants were not characterized...
  28. pmc DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex
    H Y Liu
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, NH 03824, USA
    EMBO J 16:5289-98. 1997
    ..Using the yeast two-hybrid screen, we have identified DBF2, a cell cycle-regulated protein kinase, as a CCR4-associated protein...
  29. doi Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1
    Jose Antonio Rodriguez-Rodriguez
    Cell Cycle Group, Cancer Epigenetics and Biology Program PEBC, Bellvitge Biomedical Research Institute IDIBELL, Avenida Gran Via de L Hospitalet 199 203, 08908 L Hospitalet de Llobregat, Barcelona, Spain
    Cell Rep 15:2050-62. 2016
    ..Cdk1 first phosphorylates Cdc5 to activate it in early anaphase, and then, in late anaphase, further phosphorylation of Cdc5 by Cdk1 is needed to promote its MEN-related functions. ..
  30. pmc Aim44p regulates phosphorylation of Hof1p to promote contractile ring closure during cytokinesis in budding yeast
    Dana M Alessi Wolken
    Department of Pathology and Cell Biology, Columbia University, New York, NY 10027 School of Engineering and Science, Jacobs University Bremen, 28759 Bremen, Germany
    Mol Biol Cell 25:753-62. 2014
    ..Our studies reveal a novel role for Aim44p in regulating contractile ring closure through effects on Hof1p. ..
  31. pmc Cdc14 Early Anaphase Release, FEAR, Is Limited to the Nucleus and Dispensable for Efficient Mitotic Exit
    Christopher M Yellman
    Howard Hughes Medical Institute, Chevy Chase, Maryland, 20815, United States of America Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, 06520, United States of America
    PLoS ONE 10:e0128604. 2015
    ..Our findings clarify the distinction between FEAR and MEN-dependent Cdc14 activities and will help guide emerging quantitative models of this cell cycle transition. ..
  32. pmc Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2
    Hauke Drechsler
    Biochemistry Centre Heidelberg BZH, INF 328, Heidelberg 69120, Germany
    J Cell Sci 128:3910-21. 2015
    ..We propose that yeast GSK-3 spatially controls astral MT dynamics and the loading of dynein and Kar9 on astral MT plus ends by regulating Kip2 interactions with Bim1 and MTs. ..
  33. ncbi Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex
    J Chen
    Department of Biochemistry and Molecular Biology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA
    J Mol Biol 314:683-94. 2001
    ..The distinct location of CAF40 and CAF130 and the evolutionary conservation of CAF40 implicate them in novel roles in the function of the CCR4-NOT complex...
  34. pmc Spindle pole bodies exploit the mitotic exit network in metaphase to drive their age-dependent segregation
    Manuel Hotz
    Institute of Biochemistry, Biology Department, ETH Zurich, 8093 Zurich, Switzerland
    Cell 148:958-72. 2012
    ..Consistent with this, phosphorylation of Kar9 by the MEN kinases Dbf2 and Dbf20 is not required to break Kar9 symmetry but is instead required to maintain stable association of Kar9 ..
  35. pmc Control of the mitotic exit network during meiosis
    Michelle A Attner
    David H Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Biol Cell 23:3122-32. 2012
    ....
  36. pmc Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction
    Ying Chou Chen
    Van Andel Research Institute, Grand Rapids, Michigan 49503, USA
    J Biol Chem 285:41244-54. 2010
    ..This study, therefore, details the molecular nature of a new type of PBD binding and reveals that Cdc5 targeting to phosphorylated substrates likely regulates spindle dynamics...
  37. pmc The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase
    Valentina Rossio
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, 20126 Milano, Italy
    J Cell Biol 191:981-97. 2010
    ..Our data suggest that fine-tuning regulators of mitotic exit have important functions during mitotic progression in cells treated with microtubule poisons and might be promising targets for cancer treatment...
  38. pmc The Spo12 protein of Saccharomyces cerevisiae: a regulator of mitotic exit whose cell cycle-dependent degradation is mediated by the anaphase-promoting complex
    R Shah
    National Institute for Medical Research, Medical Research Council, London NW7 1AA, United Kingdom
    Genetics 159:965-80. 2001
    ..This result is the first indication that Spo12 may carry out the same biochemical function in mitosis as it does in meiosis...
  39. pmc Mutual regulation of cyclin-dependent kinase and the mitotic exit network
    Cornelia König
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, ZMBH DKFZ Alliance, 69120 Heidelberg, Germany
    J Cell Biol 188:351-68. 2010
    ..In addition, Cdk1 phosphorylates the Mob1 protein to inhibit the activity of Dbf2-Mob1 kinase that regulates Cdc14 phosphatase...
  40. pmc Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1
    Marco Geymonat
    Division of Stem Cell Biology and Developmental Genetics, National Institute for Medical Research, London NW7 1AA, England, UK
    J Cell Biol 187:497-511. 2009
    ..We observed that lte1 mutants display aberrant localization of the polarity cap, which is the organizer of the actin cytoskeleton. We propose that Lte1's role in cell polarization underlies its contribution to mitotic regulation...
  41. ncbi A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance
    Vasso Makrantoni
    Centre for Biomolecular Sciences, School of Biology, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK
    Microbiology 153:4016-26. 2007
    ..acid hypersensitivity and impaired vacuolar acidification, phenotypes also observed in both a kinase-inactive dbf2 mutant and cells completely lacking DBF2 (dbf2Delta)...
  42. pmc SPO12 and SIT4 suppress mutations in DBF2, which encodes a cell cycle protein kinase that is periodically expressed
    V Parkes
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    Nucleic Acids Res 20:5617-23. 1992
    To help clarify the role of DBF2, a previously described cell cycle protein kinase, high copy number suppressors of the dbf2 mutation were isolated. Three open reading frames (ORF) have been identified...
  43. ncbi Characterization of CAF4 and CAF16 reveals a functional connection between the CCR4-NOT complex and a subset of SRB proteins of the RNA polymerase II holoenzyme
    H Y Liu
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, New Hampshire 03824
    J Biol Chem 276:7541-8. 2001
    ..9-MDa complex. CAF4 also displayed physical interactions with multiple CCR4-NOT components and with DBF2, a likely component of the 1.9-MDa complex...
  44. pmc Glucose and ras activity influence the ubiquitin ligases APC/C and SCF in Saccharomyces cerevisiae
    S Irniger
    Institute of Microbiology and Genetics, Georg August University, D 37077 Gottingen, Germany
    Genetics 154:1509-21. 2000
    ..We conclude that glucose and Ras proteins influence the APC/C and the SCF complex in an opposite manner. These ubiquitin ligases might represent novel targets for modulating cell division in response to growth conditions...
  45. ncbi Dbf2 is implicated in a Cbt1-dependent pathway following a shift from glucose to galactose or non-fermentable carbon sources in Saccharomyces cerevisiae
    N Grandin
    Yeast Cell Cycle Group, UMR CNRS ENS No 8510, Ecole Normale Superieure de Lyon, France
    Mol Gen Genet 261:402-7. 1999
    b>Dbf2, a cell cycle-regulated protein kinase, has been shown recently to be part of the CCR4 transcriptional regulatory complex in the yeast Saccharomyces cerevisiae...
  46. ncbi DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase
    P J Fitzpatrick
    Division of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    Mol Gen Genet 258:437-41. 1998
    ..Overexpression of Sicl, a cyclin-dependent protein kinase inhibitor, is able to suppress telophase mutants such as dbf2, cdc5 and cdc15, but not cdc14...
  47. ncbi The Cdc14 phosphatase is functionally associated with the Dbf2 protein kinase in Saccharomyces cerevisiae
    N Grandin
    Yeast Cell Cycle Group, Ecole Normale Superieure de Lyon, UMR CNRS 49, France
    Mol Gen Genet 258:104-16. 1998
    The Saccharomyces cerevisiae Cdc14 protein phosphatase and Dbf2 protein kinase have been implicated to act during late M phase, but their functions are not known...
  48. pmc DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein
    S I Komarnitsky
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham 03824, USA
    Mol Cell Biol 18:2100-7. 1998
    The DBF2 gene of the budding yeast Saccharomyces cerevisiae encodes a cell cycle-regulated protein kinase that plays an important role in the telophase/G1 transition...
  49. pmc The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively
    H Y Liu
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham 03824, USA
    EMBO J 17:1096-106. 1998
    The CCR4 transcriptional regulatory complex consisting of CCR4, CAF1, DBF2 and other unidentified factors is one of several groups of proteins that affect gene expression...
  50. pmc A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5
    K Kitada
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    Mol Cell Biol 13:4445-57. 1993
    ..CDC5 on a multicopy plasmid also suppresses temperature-sensitive cdc15, cdc20, and dbf2 mutations which affect mitosis during the cell cycle.
  51. pmc Spo12 is a limiting factor that interacts with the cell cycle protein kinases Dbf2 and Dbf20, which are involved in mitotic chromatid disjunction
    J H Toyn
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, United Kingdom
    Genetics 135:963-71. 1993
    The DBF2 and DBF20 genes of the budding yeast Saccharomyces cerevisiae encode a pair of structurally similar protein kinases. Although yeast with either gene deleted is viable, deletion of both genes is lethal...
  52. ncbi The cell-cycle-regulated budding yeast gene DBF2, encoding a putative protein kinase, has a homologue that is not under cell-cycle control
    J H Toyn
    Laboratory of Cell Propagation, National Institute for Medical Research, The Ridgeway, Mill Hill, London, U K
    Gene 104:63-70. 1991
    The budding yeast cell-cycle gene, DBF2, encoding a putative protein kinase, was shown to have a homologue, designated DBF20...