CLA4

Summary

Gene Symbol: CLA4
Description: serine/threonine protein kinase CLA4
Alias: ERC10
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. ncbi Nucleocytosolic acetyl-coenzyme a synthetase is required for histone acetylation and global transcription
    Hidekazu Takahashi
    High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Cell 23:207-17. 2006
  2. pmc Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway
    D C Raitt
    Dana Farber Cancer Institute, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 19:4623-31. 2000
  3. pmc Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4
    Matthias Versele
    Dept of Molecular and Cell Biology, University of California, Berkeley, Room 16, Barker Hall, Berkeley, CA 94720 3202, USA
    J Cell Biol 164:701-15. 2004
  4. pmc Septins, under Cla4p regulation, and the chitin ring are required for neck integrity in budding yeast
    Martin Schmidt
    National Institute of Diabetes and Digestive and Kidney Diseases, Laboratory of Biochemistry and Genetics, Bethesda, Maryland 20892, USA
    Mol Biol Cell 14:2128-41. 2003
  5. pmc Budding yeast PAK kinases regulate mitotic exit by two different mechanisms
    Elena Chiroli
    Dipartimento di Biotecnologie e Bioscienze, Piazza della Scienza 2, 20126 Milano, Italy
    J Cell Biol 160:857-74. 2003
  6. pmc Coupling morphogenesis to mitotic entry
    Krisada Sakchaisri
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:4124-9. 2004
  7. pmc Functional characterization of Dma1 and Dma2, the budding yeast homologues of Schizosaccharomyces pombe Dma1 and human Chfr
    Roberta Fraschini
    Dipartimento di Biotecnologie e Bioscienze, Piazza della Scienza 2, 20126 Milan, Italy
    Mol Biol Cell 15:3796-810. 2004
  8. pmc A role for cell polarity proteins in mitotic exit
    Thomas Höfken
    The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK
    EMBO J 21:4851-62. 2002
  9. pmc Genetic interactions among regulators of septin organization
    Amy S Gladfelter
    Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, NC 27710, USA
    Eukaryot Cell 3:847-54. 2004
  10. pmc Septin ring assembly requires concerted action of polarisome components, a PAK kinase Cla4p, and the actin cytoskeleton in Saccharomyces cerevisiae
    Jun Kadota
    Division of Molecular Interaction, Institute for Genetic Medicine, Hokkaido University Graduate School of Medicine, N15 W7, Kita ku, Sapporo, 060 0815, Japan
    Mol Biol Cell 15:5329-45. 2004

Scientific Experts

  • Robert B Annan
  • Martin Schmidt
  • Thomas Höfken
  • L J Oehlen
  • Ana Traven
  • Roberta Fraschini
  • Junwon Kim
  • Franz Meitinger
  • Maryna Gorelik
  • Meng Lin
  • Gislene Pereira
  • Giovanna Lucchini
  • Simonetta Piatti
  • Xiang Dong Gao
  • Ursula Just
  • Huan Wu
  • Jeremy Thorner
  • Mark D Rose
  • Birgit Hub
  • T J Richman
  • Sarah E Smith
  • Bahtiyar Kurtulmus
  • Noelia Blanco
  • Smita Amarnath
  • Laura Merlini
  • Satoshi Asano
  • Krisada Sakchaisri
  • Alan R Davidson
  • Michael A McMurray
  • Daniela Trinca Bertazzi
  • Kyle A Havens
  • Michael R Logan
  • Karen Artiles
  • George F Sprague
  • Clinton R Bartholomew
  • Himanshu Sinha
  • Lukasz Kozubowski
  • Alicia G Howe
  • Christopher J R Loewen
  • Satoe Takahashi
  • Melanie Heinrich
  • Christopher Tiedje
  • Kazuo Tatebayashi
  • Aster Legesse-Miller
  • Abdul Kader Souid
  • D J Lew
  • Jorrit M Enserink
  • Daniel J Lew
  • Hidekazu Takahashi
  • Trevin R Zyla
  • Charles Boone
  • Erfei Bi
  • Anupama Seshan
  • Timothy D Veenstra
  • Kyung S Lee
  • Li Rong Yu
  • Jung Eun Park
  • Melanie L Mayer
  • Jun Kadota
  • Amy S Gladfelter
  • Matthias Versele
  • Tamara J Richman
  • Masayuki Iwase
  • D I Johnson
  • Elena Chiroli
  • Megan E Keniry
  • April S Goehring
  • Jia Guo
  • Ya Ting Zhou
  • J R Pringle
  • Gregory R Smith
  • Anton Khmelinskii
  • Saravanan Palani
  • Michael Knop
  • Gilles Charvin
  • Sandrine Morlot
  • Amparo Andrés-Pons
  • G C Chen
  • Wolfgang Seufert
  • Rong Li
  • Jay R Unruh
  • Brian D Slaughter
  • Inês Mendes Pinto
  • Heidi Richter
  • Sabrina Heisel
  • Boris Rubinstein
  • J J Moskow
  • A S Gladfelter
  • Javier Arroyo
  • Enrico Cabib

Detail Information

Publications73

  1. ncbi Nucleocytosolic acetyl-coenzyme a synthetase is required for histone acetylation and global transcription
    Hidekazu Takahashi
    High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Cell 23:207-17. 2006
    ..Thus, acetyl-CoA metabolism is directly linked to chromatin regulation and may affect diverse cellular processes in which acetylation and metabolism intersect, such as disease states and aging...
  2. pmc Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway
    D C Raitt
    Dana Farber Cancer Institute, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 19:4623-31. 2000
    ..Since activated Cdc42 translocates Ste20 to sites of polarized growth, the upstream and downstream elements of the HOG pathway are brought together through the membrane targeting function of Sho1 and Cdc42...
  3. pmc Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4
    Matthias Versele
    Dept of Molecular and Cell Biology, University of California, Berkeley, Room 16, Barker Hall, Berkeley, CA 94720 3202, USA
    J Cell Biol 164:701-15. 2004
    ..Absence of a p21-activated protein kinase (Cla4) perturbs septin collar formation...
  4. pmc Septins, under Cla4p regulation, and the chitin ring are required for neck integrity in budding yeast
    Martin Schmidt
    National Institute of Diabetes and Digestive and Kidney Diseases, Laboratory of Biochemistry and Genetics, Bethesda, Maryland 20892, USA
    Mol Biol Cell 14:2128-41. 2003
    b>CLA4, encoding a protein kinase of the PAK type, and CDC11, encoding a septin, were isolated in a screen for synthetic lethality with CHS3, which encodes the chitin synthase III catalytic moiety...
  5. pmc Budding yeast PAK kinases regulate mitotic exit by two different mechanisms
    Elena Chiroli
    Dipartimento di Biotecnologie e Bioscienze, Piazza della Scienza 2, 20126 Milano, Italy
    J Cell Biol 160:857-74. 2003
    We report the characterization of the dominant-negative CLA4t allele of the budding yeast CLA4 gene, encoding a member of the p21-activated kinase (PAK) family of protein kinases, which, together with its homologue STE20, plays an ..
  6. pmc Coupling morphogenesis to mitotic entry
    Krisada Sakchaisri
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:4124-9. 2004
    ..Here, we show that two other kinases targeted sequentially to the neck, Cla4/PAK and Cdc5/Polo, are responsible for stepwise phosphorylation and down-regulation of Swe1...
  7. pmc Functional characterization of Dma1 and Dma2, the budding yeast homologues of Schizosaccharomyces pombe Dma1 and human Chfr
    Roberta Fraschini
    Dipartimento di Biotecnologie e Bioscienze, Piazza della Scienza 2, 20126 Milan, Italy
    Mol Biol Cell 15:3796-810. 2004
    ..Although their primary functions remain to be defined, our data suggest that Dma1 and Dma2 might be required to ensure timely MEN activation in telophase...
  8. pmc A role for cell polarity proteins in mitotic exit
    Thomas Höfken
    The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK
    EMBO J 21:4851-62. 2002
    ..The Rho-like GTPase Cdc42, its GEF Cdc24 and its effector Cla4 [a member of the p21-activated kinases (PAKs)] control the initial binding and activation of Lte1 to the bud cortex...
  9. pmc Genetic interactions among regulators of septin organization
    Amy S Gladfelter
    Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, NC 27710, USA
    Eukaryot Cell 3:847-54. 2004
    ..strains containing single or multiple mutations in eight septin organization genes (CDC42, RGA1, RGA2, BEM3, CLA4, GIN4, NAP1, and ELM1)...
  10. pmc Septin ring assembly requires concerted action of polarisome components, a PAK kinase Cla4p, and the actin cytoskeleton in Saccharomyces cerevisiae
    Jun Kadota
    Division of Molecular Interaction, Institute for Genetic Medicine, Hokkaido University Graduate School of Medicine, N15 W7, Kita ku, Sapporo, 060 0815, Japan
    Mol Biol Cell 15:5329-45. 2004
    ..In bni1Delta cla4-75-td mutant, septins were recruited to the incipient budding site...
  11. ncbi Phosphorylation of the Cdc42 exchange factor Cdc24 by the PAK-like kinase Cla4 may regulate polarized growth in yeast
    M P Gulli
    Swiss Institute for Experimental Cancer Research Chemin des Boveresses 155 1066 Epalinges, Vaud, Switzerland
    Mol Cell 6:1155-67. 2000
    ..activated Cdc42 then polarizes the cytoskeleton in a manner dependent on its effectors Bni1 and the PAK-like kinase Cla4. In addition, Cla4 induces phosphorylation of Cdc24, leading to its dissociation from Bem1 at bud tips, thereby ..
  12. pmc Concerted mechanism of Swe1/Wee1 regulation by multiple kinases in budding yeast
    Satoshi Asano
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    EMBO J 24:2194-204. 2005
    ..We propose that the concerted action of Cdc28/Cdk1 and Cdc5/Polo on their common substrates is an evolutionarily conserved mechanism that is crucial for effectively triggering mitotic entry and other critical mitotic events...
  13. ncbi Chemical genetic analysis of the budding-yeast p21-activated kinase Cla4p
    E L Weiss
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Nat Cell Biol 2:677-85. 2000
    ..Here we define the in vivo function of the kinase activity of the budding yeast PAK Cla4p, using cla4 alleles that are specifically inhibited by a cell-permeable compound that does not inhibit the wild-type kinase...
  14. pmc Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae
    M S Longtine
    Department of Biology and Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Mol Cell Biol 20:4049-61. 2000
    ..in three other genes (GIN4, encoding a kinase related to the Schizosaccharomyces pombe mitotic inducer Nim1p; CLA4, encoding a p21-activated kinase; and NAP1, encoding a Clb2p-interacting protein) also produce perturbations of ..
  15. pmc Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit
    Roberta Fraschini
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, 20126 Milano, Italy
    J Cell Biol 172:335-46. 2006
    ..Moreover, it correlates with the passage of one spindle pole through the bud neck because it needs septin ring formation and bud neck kinases...
  16. pmc The elm1 kinase functions in a mitotic signaling network in budding yeast
    A Sreenivasan
    Sinsheimer Laboratories, Department of Biology, University of California, Santa Cruz, California 95064, USA
    Mol Cell Biol 19:7983-94. 1999
    ..the function of a Clb2-binding protein called Nap1, the Cdc42 GTPase, and two protein kinases called Gin4 and Cla4. In this study, we demonstrate that the Elm1 kinase also plays a role in the control of bud growth during mitosis...
  17. pmc PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae
    S P Holly
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 147:845-56. 1999
    ..Inactivation of the PAK homologues Ste20 and Cla4 at various points in the cell cycle resulted in loss of cell and actin cytoskeletal polarity, but not in ..
  18. pmc Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle
    Xiang Dong Gao
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Mol Biol Cell 18:2542-60. 2007
    ..Together, these results suggest that Axl2p plays sequential and distinct roles in the regulation of cellular morphogenesis in yeast cell cycle...
  19. pmc Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex
    Lukasz Kozubowski
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 18:1719-26. 2008
    ..Cdc42p concentrates at a random cortical site during symmetry breaking in a manner that requires the scaffold protein Bem1p. The mechanism whereby Bem1p promotes this polarization was unknown...
  20. pmc The Rts1 regulatory subunit of protein phosphatase 2A is required for control of G1 cyclin transcription and nutrient modulation of cell size
    Karen Artiles
    Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America
    PLoS Genet 5:e1000727. 2009
    ..Together, these observations demonstrate that Rts1 is a key player in pathways that link nutrient availability, cell size, and G1 cyclin transcription. Since Rts1 is highly conserved, it may function in similar pathways in vertebrates...
  21. pmc The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4
    Daniela Trinca Bertazzi
    DKFZ ZMBH Alliance, German Cancer Research Center, 69120 Heidelberg, Germany
    J Cell Biol 193:1033-48. 2011
    ..This Lte1-mediated exclusion of Kin4 from the dSPB is essential for proper mitotic exit of cells with a correctly aligned spindle. Therefore, Lte1 promotes mitotic exit by inhibiting Kin4 activity at the dSPB...
  22. ncbi The phosphorylation site for Ste20p-like protein kinases is essential for the function of myosin-I in yeast
    C Wu
    Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2, Canada
    J Biol Chem 272:30623-6. 1997
    ..Our results suggest that phosphorylation of the head domain by Ste20p-like protein kinases plays an essential role in the function of myosin-I in yeast cells...
  23. pmc The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae
    G C Chen
    Department of Microbiology, The University of Texas, Austin, Texas 78712, USA
    Genes Dev 11:2958-71. 1997
    ..The ability of Gic1 to associate with Cdc42 is important for its function but is apparently not essential for its subcellular localization...
  24. ncbi Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast
    F Cvrckova
    Institute of Molecular Pathology IMP, Wien, Austria
    Genes Dev 9:1817-30. 1995
    ..Mammalian homologs of Ste20 exist, but their function remains unknown. We identified a novel yeast STE20 homolog, CLA4, in a screen for mutations lethal in the absence of the G1 cyclins Cln1 and Cln2...
  25. ncbi Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p
    I Bose
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 276:7176-86. 2001
    ..We suggest that Bem1p acts to concentrate polarity establishment proteins at a discrete site, facilitating polarization and promoting Cdc24p phosphorylation at specific times during the cell cycle...
  26. pmc The phosphotyrosyl phosphatase activator, Ncs1p (Rrd1p), functions with Cla4p to regulate the G(2)/M transition in Saccharomyces cerevisiae
    D A Mitchell
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Mol Cell Biol 21:488-500. 2001
    ..To learn more about the shared function, we sought new mutations that were lethal in the absence of CLA4. This approach led to the identification of at least 10 complementation groups designated NCS (need CLA4 to survive)..
  27. ncbi Control of mitotic events by the Cdc42 GTPase, the Clb2 cyclin and a member of the PAK kinase family
    H Tjandra
    Sinsheimer Laboratories, Department of Biology, University of California, Santa Cruz 95064, USA
    Curr Biol 8:991-1000. 1998
    ..A protein called Nap1 is required in vivo for the activation of Gin4, and is able to bind to both Gin4 and Clb2. We have used a simple genetic screen to identify additional proteins that function in this pathway...
  28. pmc Cla4p, a Saccharomyces cerevisiae Cdc42p-activated kinase involved in cytokinesis, is activated at mitosis
    B K Benton
    The Rockefeller University, New York, New York 10021, USA
    Mol Cell Biol 17:5067-76. 1997
    ..Mutations in GIN4 and CLA4 were isolated in a screen for mutants that are inviable with deletions in the G1 cyclins CLN1 and CLN2...
  29. pmc Isolation and characterization of effector-loop mutants of CDC42 in yeast
    A S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Biol Cell 12:1239-55. 2001
    ..The availability of partial function alleles of CDC42 in a genetically tractable system serves as a useful starting point for genetic approaches to identify such novel effectors...
  30. pmc Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling
    Junwon Kim
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Genet 11:e1005684. 2015
    ..Taken together, our findings show that budding yeast can access a stable constitutive pseudohyphal growth state with very few genetic and regulatory changes. ..
  31. pmc A biochemical genomics screen for substrates of Ste20p kinase enables the in silico prediction of novel substrates
    Robert B Annan
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    PLoS ONE 4:e8279. 2009
    ....
  32. pmc A mechanism for the coordination of proliferation and differentiation by spatial regulation of Fus2p in budding yeast
    Junwon Kim
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Genes Dev 26:1110-21. 2012
    ..Thus, potential premature activation of Fus2p in mitosis is prevented by cell cycle-dependent phosphorylation that overrides the mating pheromone-induced phosphorylation that drives nuclear export...
  33. pmc Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42
    Sarah E Smith
    Stowers Institute for Medical Research, Kansas City, MO, 64110
    J Cell Biol 202:1091-106. 2013
    ..The model predicts a nonmonotonic dependence of cell polarization on the concentration of Rdi1 relative to that of Cdc42. ..
  34. doi A memory system of negative polarity cues prevents replicative aging
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia, German Cancer Research Center DKFZ, DKFZ ZMBH Alliance, Im Neuenheimer Feld 581, Heidelberg 69120, Germany
    Cell 159:1056-69. 2014
    ..Our work thus established CRMs as negative polarity cues that prevent Cdc42 reactivation to sustain the fitness of replicating cells. ..
  35. pmc The anillin-related region of Bud4 is the major functional determinant for Bud4's function in septin organization during bud growth and axial bud site selection in budding yeast
    Huan Wu
    Department of Microbiology, College of Life Sciences, Wuhan University, Wuhan, China
    Eukaryot Cell 14:241-51. 2015
    ..These observations provide new insights into the interaction of Bud4 with the septins and Bud3. ..
  36. pmc A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia Unit, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany
    PLoS Biol 11:e1001495. 2013
    ..Our findings provide unexpected insights into the temporal control of cytokinesis and describe the importance of a Gps1-dependent mechanism for highly accurate polarity switching between two closely connected locations...
  37. pmc Crosslinks in the cell wall of budding yeast control morphogenesis at the mother-bud neck
    Noelia Blanco
    Departamento de Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain
    J Cell Sci 125:5781-9. 2012
    Previous work has shown that, in cla4Δ cells of budding yeast, where septin ring organization is compromised, the chitin ring at the mother-daughter neck becomes essential for prevention of neck widening and for cytokinesis...
  38. pmc Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the Elm1 kinase to the bud neck
    Laura Merlini
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS Genet 8:e1002670. 2012
    ..Altogether, our data indicate that septin dynamics and SPOC function are intimately linked and support the idea that integrity of the bud neck is crucial for SPOC signalling...
  39. pmc Pleiotropic roles of a ribosomal protein in Dictyostelium discoideum
    Smita Amarnath
    Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India
    PLoS ONE 7:e30644. 2012
    ..We speculate that the existence of the second role can act as a safeguard against perturbations to ribosome function caused by spontaneous variations in DdS4 levels...
  40. pmc Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface
    Maryna Gorelik
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    J Biol Chem 287:9168-77. 2012
    ..Remarkably, this modulation of local binding energetics can explain the distinct and highly nuanced binding specificities of these two domains...
  41. doi Cdc42p and Rho1p are sequentially activated and mechanistically linked to vacuole membrane fusion
    Michael R Logan
    Department of Cell Biology, University of Alberta, Edmonton, Alta, Canada
    Biochem Biophys Res Commun 394:64-9. 2010
    ..These results define unique activation mechanisms for Cdc42p and Rho1p, which may be linked to the vacuole membrane fusion mechanism...
  42. pmc Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast
    Himanshu Sinha
    European Molecular Biology Laboratory, Heidelberg, Germany
    Genetics 180:1661-70. 2008
    ..The targeted backcrossing approach overcomes the difficulties posed by sample size, genetic linkage, and epistatic effects and facilitates identification of additional alleles with smaller contributions to complex traits...
  43. pmc p21-activated kinases Cla4 and Ste20 regulate vacuole inheritance in Saccharomyces cerevisiae
    Clinton R Bartholomew
    Department of Cell and Developmental Biology, Vanderbilt School of Medicine, Nashville, TN 37232 8240, USA
    Eukaryot Cell 8:560-72. 2009
    ..The p21-activated kinases (PAKs) Cla4 and Ste20 are localized to and activated in the bud...
  44. pmc The Ccr4-Pop2-NOT mRNA deadenylase contributes to septin organization in Saccharomyces cerevisiae
    Ana Traven
    St Vincent s Institute of Medical Research, Fitzroy, Victoria 3065, Australia
    Genetics 182:955-66. 2009
    ..mRNAs encoding regulators of septin assembly (Ccd42, Cdc24, Rga1, Rga2, Bem3, Gin4, Cla4, and Elm1) presented with short poly(A) tails at steady state in wild-type (wt) cells, suggesting their translation ..
  45. pmc The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway
    Meng Lin
    Institute of Biochemistry, Christian Albrecht University, 24098 Kiel, Germany
    Mol Biol Cell 20:4826-37. 2009
    ..GTPase Cdc42 regulates polarized growth through its effectors, including the p21-activated kinases (PAKs) Ste20, Cla4, and Skm1...
  46. ncbi Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases
    T Leeuw
    Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    Nature 391:191-5. 1998
    ....
  47. ncbi Potential regulation of Ste20 function by the Cln1-Cdc28 and Cln2-Cdc28 cyclin-dependent protein kinases
    L J Oehlen
    Rockefeller University, New York, New York 10021, USA
    J Biol Chem 273:25089-97. 1998
    ....
  48. ncbi Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae
    Meng Lin
    Institute of Biochemistry, Christian Albrecht University, Kiel, Germany
    FEBS J 276:7253-64. 2009
    ..b>CLA4 interacts genetically with ERG4 and NCP1...
  49. pmc Slk19p of Saccharomyces cerevisiae regulates anaphase spindle dynamics through two independent mechanisms
    Kyle A Havens
    Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Genetics 186:1247-60. 2010
    ....
  50. pmc A Conserved residue in the yeast Bem1p SH3 domain maintains the high level of binding specificity required for function
    Maryna Gorelik
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    J Biol Chem 286:19470-7. 2011
    ..This work provides a clear illustration of the importance of intrinsic binding specificity for the function of protein-protein interaction modules, and the key role of "negative" interactions in determining the specificity of a domain...
  51. ncbi Analysis of the mechanisms of action of the Saccharomyces cerevisiae dominant lethal cdc42G12V and dominant negative cdc42D118A mutations
    C R Davis
    Department of Microbiology and Molecular Genetics and the Markey Center for Molecular Genetics, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 273:849-58. 1998
    ....
  52. pmc Genetic interactions with mutations affecting septin assembly reveal ESCRT functions in budding yeast cytokinesis
    Michael A McMurray
    Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    Biol Chem 392:699-712. 2011
    ..We show here that mutations in ESCRT-encoding genes exacerbate the cytokinesis defects of cla4Δ or elm1Δ mutants, in which septin assembly is perturbed at an early stage in cell division, and alleviate ..
  53. pmc ELM1 is required for multidrug resistance in Saccharomyces cerevisiae
    Abdul Kader Souid
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    Genetics 173:1919-37. 2006
    ..Collectively, these data show that ELM1, and genes encoding related serine/threonine protein kinases, are required for regulation of multidrug resistance involving, at least in part, control of PDR5 transcription...
  54. pmc Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast
    April S Goehring
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Mol Biol Cell 14:4329-41. 2003
    ..Moreover, these five genes have a role in invasive growth and display genetic interactions with the TOR pathway. In summary, our results suggest the urmylation pathway is involved in nutrient sensing and budding...
  55. pmc Identification of p21-activated kinase specificity determinants in budding yeast: a single amino acid substitution imparts Ste20 specificity to Cla4
    Megan E Keniry
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Mol Cell Biol 23:1569-80. 2003
    Two closely related p21-activated kinases from Saccharomyces cerevisiae, Ste20 and Cla4, interact with and are regulated by Cdc42, a small Rho-like GTPase...
  56. ncbi Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae
    Masayuki Iwase
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan
    Cell Struct Funct 29:1-15. 2004
    ..In view of the fact that members belonging to the mitotic signaling network are localized to the bud neck, at least at some stage of the cell cycle, Rei1 is a unique component of this pathway...
  57. ncbi Analysis of cell-cycle specific localization of the Rdi1p RhoGDI and the structural determinants required for Cdc42p membrane localization and clustering at sites of polarized growth
    Tamara J Richman
    Department of Microbiology and Molecular Genetics and the Markey Center for Molecular Genetics, University of Vermont, 95 Carrigan Dr, 202 Stafford Hall, Burlington, VT 05405, USA
    Curr Genet 45:339-49. 2004
    ..Taken together, these results provide insight into the complicated nature of the relationships between Cdc42p localization, nucleotide binding, and protein-protein interactions...
  58. pmc GTPase-activating proteins for Cdc42
    Gregory R Smith
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Eukaryot Cell 1:469-80. 2002
    ..Thus, it appears that the GAPs play a role in modulating specific aspects of Cdc42 function. Alternatively, the different phenotypes could reflect quantitative rather than qualitative differences in GAP activity in the mutant strains...
  59. pmc Different domains of the essential GTPase Cdc42p required for growth and development of Saccharomyces cerevisiae
    H U Mösch
    Institute for Microbiology and Genetics, Georg August University, D 37077 Gottingen, Germany
    Mol Cell Biol 21:235-48. 2001
    ....
  60. ncbi Ras and the Rho effector Cla4 collaborate to target and anchor Lte1 at the bud cortex
    Anupama Seshan
    Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Cell Cycle 4:940-6. 2005
    ..Ras, the Rho GTPase Cdc42 and its effector the protein kinase Cla4 are required for Lte1's association with the bud cortex...
  61. pmc Saccharomyces cerevisiae cdc42p GTPase is involved in preventing the recurrence of bud emergence during the cell cycle
    T J Richman
    Department of Microbiology and Molecular Genetics and Markey Center for Molecular Genetics, University of Vermont Burlington, Vermont 05405, USA
    Mol Cell Biol 20:8548-59. 2000
    ....
  62. pmc Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast
    E Bi
    Department of Biology and Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Biol Cell 11:773-93. 2000
    ..designed to identify possible additional targets of Cdc24p instead identified two previously known genes, MSB1 and CLA4, and one novel gene, designated MSB3, all of which appear to function in the Cdc24p-Cdc42p pathway...
  63. pmc Regulated phosphorylation of budding yeast's essential myosin V heavy chain, Myo2p
    Aster Legesse-Miller
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Mol Biol Cell 17:1812-21. 2006
    ..These results suggest that in yeast, Myo2p is subject to phosphoregulation involving a PKA-related signaling pathway...
  64. pmc Identification of protein complexes required for efficient sister chromatid cohesion
    Melanie L Mayer
    Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4
    Mol Biol Cell 15:1736-45. 2004
    ..Furthermore, we find that genes involved in mitotic spindle integrity and positioning have a previously unrecognized role in sister chromatid cohesion...
  65. pmc Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway
    Kazuo Tatebayashi
    Division of Molecular Cell Signaling, Institute of Medical Sciences, University of Tokyo, Tokyo, Japan
    EMBO J 25:3033-44. 2006
    ..We found that Cdc42, in addition to binding and activating the PAK-like kinases Ste20 and Cla4, binds to the Ste11-Ste50 complex to bring activated Ste20/Cla4 to their substrate Ste11...
  66. pmc Checkpoint proteins control morphogenetic events during DNA replication stress in Saccharomyces cerevisiae
    Jorrit M Enserink
    Ludwig Institute for Cancer Research, University of California, San Diego School of Medicine, La Jolla, CA 92093, USA
    J Cell Biol 175:729-41. 2006
    ..Thus, checkpoint proteins play an important role in coordinating morphogenetic events with DNA replication during replication stress...
  67. pmc Role of Cdc42-Cla4 interaction in the pheromone response of Saccharomyces cerevisiae
    Melanie Heinrich
    Department of Biology, Molecular Genetics, Philipps University, D 35032 Marburg, Germany
    Eukaryot Cell 6:317-27. 2007
    ..Furthermore, Cdc42(V36A) and Cdc42(V36A, I182T) proteins show reduced interaction with the PAK Cla4 but not with Ste20...
  68. ncbi The Cdc42p GTPase is involved in a G2/M morphogenetic checkpoint regulating the apical-isotropic switch and nuclear division in yeast
    T J Richman
    Department of Microbiology and Molecular Genetics and the Markey Center for Molecular Genetics, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 274:16861-70. 1999
    ....
  69. pmc Regulation of phosphoinositide levels by the phospholipid transfer protein Sec14p controls Cdc42p/p21-activated kinase-mediated cell cycle progression at cytokinesis
    Alicia G Howe
    Atlantic Research Centre, Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada
    Eukaryot Cell 6:1814-23. 2007
    ..of phosphatidylinositol 4-kinases and phosphatidylinositol 4-phosphate 5-kinase prevented growth arrest by CDC42, CLA4, or STE20 upon inactivation of Sec14p function...
  70. ncbi Proteins involved in sterol synthesis interact with Ste20 and regulate cell polarity
    Christopher Tiedje
    Institute of Biochemistry, Christian Albrecht University Kiel, Olshausenstrasse 40, 24098 Kiel, Germany
    J Cell Sci 120:3613-24. 2007
    ..Lack of CBR1 produced no detectable phenotype, whereas the deletion of CBR1 in the absence of NCP1 was lethal. Using a conditional lethal mutant we demonstrate that both proteins have overlapping functions in bud morphology...
  71. pmc Identification of novel membrane-binding domains in multiple yeast Cdc42 effectors
    Satoe Takahashi
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 18:4945-56. 2007
    ....
  72. pmc Inheritance of cortical ER in yeast is required for normal septin organization
    Christopher J R Loewen
    Division of Cell Biology, University College London Institute of Ophthalmology, London EC1V 9EL, England, UK
    J Cell Biol 179:467-83. 2007
    ..This perturbation leads to a delay in the transition through G2, activating the Saccharomyces wee1 kinase (Swe1) and the morphogenesis checkpoint. Thus, we identify a mechanism involved in sensing the distribution of ER...
  73. doi Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3
    Martin Schmidt
    Department of Biochemistry and Nutrition, Des Moines University, Des Moines, IA 50311, USA
    FEMS Yeast Res 8:362-73. 2008
    ..In all of the above aspects, the cak1P212S mutants are similar to previously described cla4 mutants, suggesting that the cell wall defects are common to mutants with hyperpolarized growth...