BRE1

Summary

Gene Symbol: BRE1
Description: E3 ubiquitin-protein ligase BRE1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Direct Bre1-Paf1 complex interactions and RING finger-independent Bre1-Rad6 interactions mediate histone H2B ubiquitylation in yeast
    Jaehoon Kim
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10065, USA
    J Biol Chem 284:20582-92. 2009
  2. ncbi Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St Louis, MO 63104, USA
    Mol Cell 11:267-74. 2003
  3. ncbi The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 278:34739-42. 2003
  4. ncbi H2B ubiquitylation acts as a barrier to Ctk1 nucleosomal recruitment prior to removal by Ubp8 within a SAGA-related complex
    Anastasia Wyce
    Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, PA 19104, USA
    Mol Cell 27:275-88. 2007
  5. pmc The RAD6/BRE1 histone modification pathway in Saccharomyces confers radiation resistance through a RAD51-dependent process that is independent of RAD18
    John C Game
    Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA
    Genetics 173:1951-68. 2006
  6. pmc Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9
    Robert Wysocki
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 25:8430-43. 2005
  7. ncbi BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex
    R Nicholas Laribee
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Curr Biol 15:1487-93. 2005
  8. ncbi A conserved RING finger protein required for histone H2B monoubiquitination and cell size control
    William W Hwang
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    Mol Cell 11:261-6. 2003
  9. ncbi The DNA damage checkpoint response requires histone H2B ubiquitination by Rad6-Bre1 and H3 methylation by Dot1
    Michele Giannattasio
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, 20133 Milano, Italy
    J Biol Chem 280:9879-86. 2005
  10. pmc Chemo-Genetic Interactions Between Histone Modification and the Antiproliferation Drug AICAR Are Conserved in Yeast and Humans
    Delphine Albrecht
    Universite de Bordeaux, IBGC UMR 5095, F 33077 Bordeaux, France
    Genetics 204:1447-1460. 2016

Research Grants

Scientific Experts

  • John Game
  • Jaehoon Kim
  • Brian D Strahl
  • Tiaojiang Xiao
  • William W Hwang
  • Nevan J Krogan
  • Adam Wood
  • Delphine Albrecht
  • Gustavo M Silva
  • Eleonora Turco
  • Pankaj Kumar
  • Glenn G Wozniak
  • Alyssa A Rossodivita
  • R Nicholas Laribee
  • Rwik Sen
  • Brett N Tomson
  • Ali Shilatifard
  • Jack F Greenblatt
  • Kelly M Trujillo
  • Alfonso Gallego-Sánchez
  • Elia M Crisucci
  • Adam Chruscicki
  • David Faucher
  • Young Ha Song
  • Nancy Lévesque
  • Karen M Arndt
  • David Walter
  • Robert G Roeder
  • Ellen S Kats
  • Hélène Gaillard
  • Munshi Azad Hossain
  • Adeline Vitaliano-Prunier
  • Mary Ann Osley
  • Fuqiang Geng
  • Mary A Braun
  • Anastasia Wyce
  • Yoichiro Shibata
  • Akira Motegi
  • Timothy R Hughes
  • Hiten D Madhani
  • Robert Wysocki
  • Michele Giannattasio
  • Cheng Fu Kao
  • Kentaro Yamashita
  • Jessica Schneider
  • Bertrand Daignan-Fornier
  • Mark Johnston
  • Florian Gueniot
  • Benoit Pinson
  • Jim Dover
  • Johanna Ceschin
  • Jim Dompierre
  • Cynthia Wolberger
  • Alwin Köhler
  • Christine Vogel
  • Daniel Finley
  • Maren Schneider
  • Laura D Gallego
  • Andrea L Karl
  • Anna L Boudoures
  • Maria Teresa Teixeira
  • Jeffrey S Thompson
  • Jonathan P Mecoli
  • Arron M Cole
  • Megan R Ansbro
  • Elizabeth M Steenkiste
  • Eudora M Vines
  • Corey Nislow
  • Marinella Gebbia
  • Lawrence E Heisler
  • Shweta Lahudkar
  • Sukesh R Bhaumik
  • Geetha Durairaj
  • Avelino Bueno
  • Francisco Conde
  • Pedro A San-Segundo
  • Sonia Andrés
  • Christopher J R Loewen
  • Seong Hoon Ahn
  • Grace P Leung
  • Birthe Fahrenkrog
  • LeAnn J Howe
  • Thorsten I Schmidt
  • Barry P Young
  • Michael S Kobor
  • Raymund J Wellinger
  • Vicki E MacDonald
  • Anja Matter
  • Alexandra K Fok
  • Andres Aguilera

Detail Information

Publications37

  1. pmc Direct Bre1-Paf1 complex interactions and RING finger-independent Bre1-Rad6 interactions mediate histone H2B ubiquitylation in yeast
    Jaehoon Kim
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10065, USA
    J Biol Chem 284:20582-92. 2009
    ..We also find that, analogous to heteromeric complex formation by BRE1 paralogues in other organisms, yBre1 forms a homo-multimeric complex...
  2. ncbi Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St Louis, MO 63104, USA
    Mol Cell 11:267-74. 2003
    Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription...
  3. ncbi The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 278:34739-42. 2003
    Monoubiquitination of histone H2B, catalyzed by Rad6-Bre1, is required for methylation of histone H3 on lysines 4 and 79, catalyzed by the Set1-containing complex COMPASS and Dot1p, respectively...
  4. ncbi H2B ubiquitylation acts as a barrier to Ctk1 nucleosomal recruitment prior to removal by Ubp8 within a SAGA-related complex
    Anastasia Wyce
    Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, PA 19104, USA
    Mol Cell 27:275-88. 2007
    ..These findings reveal a mechanism by which H2B ubiquitylation acts as a barrier to Ctk1 association with active genes, while subsequent deubiquitylation by Ubp8 triggers Ctk1 recruitment at the appropriate point in activation...
  5. pmc The RAD6/BRE1 histone modification pathway in Saccharomyces confers radiation resistance through a RAD51-dependent process that is independent of RAD18
    John C Game
    Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA
    Genetics 173:1951-68. 2006
    ..We conclude that IR resistance conferred by BRE1 and DOT1 is mediated through homologous recombinational repair, not postreplication repair, and confirm findings of ..
  6. pmc Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9
    Robert Wysocki
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 25:8430-43. 2005
    ..Mutations that affect Dot1 function such as Rad6-Bre1/Paf1 pathway gene deletions or mutation of H2B Lys 123 or H3 Lys 79 share dot1Delta checkpoint defects...
  7. ncbi BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex
    R Nicholas Laribee
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Curr Biol 15:1487-93. 2005
    ..Our data reveal a novel function for the BUR kinase in transcriptional regulation through the selective control of histone modifications...
  8. ncbi A conserved RING finger protein required for histone H2B monoubiquitination and cell size control
    William W Hwang
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    Mol Cell 11:261-6. 2003
    ..The identity of the cognate ubiquitin ligase is unknown. We identify Bre1 as an evolutionarily conserved RING finger protein required in vivo for both H2B ubiquitination and H3 K4 ..
  9. ncbi The DNA damage checkpoint response requires histone H2B ubiquitination by Rad6-Bre1 and H3 methylation by Dot1
    Michele Giannattasio
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, 20133 Milano, Italy
    J Biol Chem 280:9879-86. 2005
    ..We demonstrate that ubiquitination of histone H2B on lysine 123 by the Rad6-Bre1 complex, is necessary for activation of Rad53 kinase and cell cycle arrest...
  10. pmc Chemo-Genetic Interactions Between Histone Modification and the Antiproliferation Drug AICAR Are Conserved in Yeast and Humans
    Delphine Albrecht
    Universite de Bordeaux, IBGC UMR 5095, F 33077 Bordeaux, France
    Genetics 204:1447-1460. 2016
    ..Here, we found that loss of several histone-modifying enzymes, including Bre1 (histone H2B ubiquitination) and Set1 (histone H3 lysine 4 methylation), greatly enhanced AICAR inhibition on ..
  11. pmc Structure of the yeast Bre1 RING domain
    Pankaj Kumar
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland, 21205
    Proteins 83:1185-90. 2015
    ..The RING E3 ligase, Bre1, catalyzes monoubiquitination of H2B in concert with the E2 ubiquitin-conjugating enzyme, Rad6...
  12. pmc K63 polyubiquitination is a new modulator of the oxidative stress response
    Gustavo M Silva
    Center for Genomics and Systems Biology, New York University, New York, New York, USA
    Nat Struct Mol Biol 22:116-23. 2015
    ..enzyme Ubp2, leading to accumulation of K63 conjugates assembled by the Rad6 ubiquitin conjugase and the Bre1 ubiquitin ligase...
  13. pmc Monoubiquitination of histone H2B is intrinsic to the Bre1 RING domain-Rad6 interaction and augmented by a second Rad6-binding site on Bre1
    Eleonora Turco
    From the Max F Perutz Laboratories, Medical University of Vienna, Vienna Biocenter VBC, Dr Bohr Gasse 9 3, 1030 Vienna, Austria
    J Biol Chem 290:5298-310. 2015
    ..The yeast RING E3 ligase Bre1 combines with the E2 Rad6 to monoubiquitinate histone H2B during transcription...
  14. pmc Catalysis-dependent stabilization of Bre1 fine-tunes histone H2B ubiquitylation to regulate gene transcription
    Glenn G Wozniak
    Curriculum in Genetics and Molecular Biology
    Genes Dev 28:1647-52. 2014
    ..Here we show in yeast that H2BK123ub1 is regulated in part through the protein stability of the E3 ubiquitin ligase Bre1. We found that Bre1 stability is controlled by the Rtf1 subunit of the polymerase-associated factor (PAF) complex ..
  15. pmc Histone H3 K79 methylation states play distinct roles in UV-induced sister chromatid exchange and cell cycle checkpoint arrest in Saccharomyces cerevisiae
    Alyssa A Rossodivita
    Department of Biology, Denison University, Granville, OH 43023, USA
    Nucleic Acids Res 42:6286-99. 2014
    ....
  16. pmc A role for H2B ubiquitylation in DNA replication
    Kelly M Trujillo
    Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Mol Cell 48:734-46. 2012
    ..of DNA replication in budding yeast, and as DNA is replicated its levels are maintained on daughter strands by the Bre1 ubiquitin ligase...
  17. pmc Reversal of PCNA ubiquitylation by Ubp10 in Saccharomyces cerevisiae
    Alfonso Gallego-Sánchez
    Instituto de Biologia Molecular y Celular del Cancer, Universidad de Salamanca CSIC, Salamanca, Spain
    PLoS Genet 8:e1002826. 2012
    ..Finally, we report that Ubp10 counteracts Rad18 E3-ubiquitin ligase activity on PCNA at lysine 164 in such a manner that deregulation of Ubp10 expression causes tolerance impairment and MMS hypersensitivity...
  18. pmc Functional analysis of Bre1p, an E3 ligase for histone H2B ubiquitylation, in regulation of RNA polymerase II association with active genes and transcription in vivo
    Rwik Sen
    Department of Biochemistry and Molecular Biology, Southern Illinois University, School of Medicine, Carbondale, Illinois 62901, USA
    J Biol Chem 288:9619-33. 2013
    ..we find here that the association of RNA polymerase II with the active GAL1 coding sequence is not decreased in Δbre1, although it is required for H2B ubiquitylation...
  19. pmc Effects of the Paf1 complex and histone modifications on snoRNA 3'-end formation reveal broad and locus-specific regulation
    Brett N Tomson
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Mol Cell Biol 33:170-82. 2013
    ..cerevisiae, implicates the participation of transcriptional proteins and histone modifications in this process, and suggests that the Paf1C contributes to the fine tuning of nuanced levels of regulation that exist at individual loci...
  20. pmc A Bre1-associated protein, large 1 (Lge1), promotes H2B ubiquitylation during the early stages of transcription elongation
    Young Ha Song
    Division of Molecular and Life Science, College of Science and Technology, Hanyang University, 1271 Sa 3 dong, Sangnok gu, Ansan, Gyeonggi do 426 791, Republic of Korea
    J Biol Chem 285:2361-7. 2010
    ..Here, we show that Lge1 (Large 1) is found in a complex containing Rad6.Bre1 and that it controls the recruitment of Bre1, a ubiquitin ligase, and Ubp8, a deubiquitylase, to promote ..
  21. pmc Methylated H3K4, a transcription-associated histone modification, is involved in the DNA damage response pathway
    David Faucher
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
    PLoS Genet 6:. 2010
    ..Given the high degree of conservation for the methyltransferase and the histone mark in a broad variety of organisms, these results could have similar implications for genome stability mechanisms in vertebrate and mammalian cells...
  22. pmc Loss of H3 K79 trimethylation leads to suppression of Rtt107-dependent DNA damage sensitivity through the translesion synthesis pathway
    Nancy Lévesque
    Department of Medical Genetics, University of British Columbia, Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, Vancouver, British Columbia V5Z 4H4, Canada
    J Biol Chem 285:35113-22. 2010
    ..These data revealed a multifaceted functional relationship between Rtt107 and Dot1 in the DNA damage response and maintenance of genome integrity...
  23. doi Bre1p-mediated histone H2B ubiquitylation regulates apoptosis in Saccharomyces cerevisiae
    David Walter
    M E Müller Institute for Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    J Cell Sci 123:1931-9. 2010
    b>BRE1 encodes an E3 ubiquitin protein ligase that is required for the ubiquitylation of histone H2B at lysine 123 (K123)...
  24. pmc Critical determinants for chromatin binding by Saccharomyces cerevisiae Yng1 exist outside of the plant homeodomain finger
    Adam Chruscicki
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Genetics 185:469-77. 2010
    ..Although these motifs can bind histones independently, together they increase the apparent association of Yng1 for the H3 tail...
  25. pmc Nonredundant requirement for multiple histone modifications for the early anaphase release of the mitotic exit regulator Cdc14 from nucleolar chromatin
    William W Hwang
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA
    PLoS Genet 5:e1000588. 2009
    ..analysis of genetic interactions, we report here a novel requirement for the ubiquitination of histone H2B by the Bre1 ubiquitin ligase in the cell cycle-dependent release of Cdc14 from nucleolar chromatin when the MEN is inactivated...
  26. pmc The Saccharomyces cerevisiae Rad6 postreplication repair and Siz1/Srs2 homologous recombination-inhibiting pathways process DNA damage that arises in asf1 mutants
    Ellen S Kats
    Ludwig Institute for Cancer Research, Departments of Medicine and Cellular and Molecular Medicine, and Biomedical Sciences Graduate Program, UC San Diego School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0669, USA
    Mol Cell Biol 29:5226-37. 2009
    ..Our results show that ASF1 probably contributes to the maintenance of genome stability through multiple mechanisms, some of which involve the PRR and HRS pathways...
  27. pmc The cap binding complex influences H2B ubiquitination by facilitating splicing of the SUS1 pre-mRNA
    Munshi Azad Hossain
    Molecular Biology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093, USA
    RNA 15:1515-27. 2009
    ....
  28. pmc RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells
    Jaehoon Kim
    The Rockefeller University, New York, NY 10065, USA
    Cell 137:459-71. 2009
    ..These studies establish the natural H2B ubiquitylation factors in human cells and also detail the mechanistic basis for H2B ubiquitylation and function during transcription...
  29. pmc Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair
    Hélène Gaillard
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    PLoS Genet 5:e1000364. 2009
    ..Our data provide evidence that subunits of the SAGA and Ccr4-Not complexes, Mediator, Bre1, Bur2, and Fun12 affect transcription elongation to different extents...
  30. doi Ubiquitylation of the COMPASS component Swd2 links H2B ubiquitylation to H3K4 trimethylation
    Adeline Vitaliano-Prunier
    Institut Jacques Monod, Universite Paris VII, Universite Paris VI, CNRS, 2 place Jussieu, Tour 43, 75251 Paris Cedex 05, France
    Nat Cell Biol 10:1365-71. 2008
    ..We found that Rad6/Bre1 ubiquitylation enzymes responsible for H2B ubiquitylation also participate directly in Swd2 modification...
  31. pmc Polyubiquitylation of histone H2B
    Fuqiang Geng
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Biol Cell 19:3616-24. 2008
    ..There are at least two distinct modes of H2B polyubiquitylation: one that occurs at K123 and depends on the Rad6-Bre1 ubiquitylation machinery and another that occurs on multiple lysine residues and is catalyzed by an uncharacterized ..
  32. pmc Rad6 plays a role in transcriptional activation through ubiquitylation of histone H2B
    Cheng Fu Kao
    University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA
    Genes Dev 18:184-95. 2004
    ..Rad6 promoter association depends on the Gal4 activator and the Rad6-associated E3 ligase, Bre1, but is independent of the histone acetyltransferase, Gcn5...
  33. pmc Identification of Rkr1, a nuclear RING domain protein with functional connections to chromatin modification in Saccharomyces cerevisiae
    Mary A Braun
    Department of Biological Sciences, University of Pittsburgh, 269 Crawford Hall, 4249 Fifth Avenue, Pittsburgh, PA 15260, USA
    Mol Cell Biol 27:2800-11. 2007
    ..Taken together, our results identify a new participant in a protein ubiquitylation pathway within the nucleus that acts to modulate chromatin function and transcription...
  34. pmc The RNA polymerase II kinase Ctk1 regulates positioning of a 5' histone methylation boundary along genes
    Tiaojiang Xiao
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, 405 Mary Ellen Jones, Chapel Hill, NC 27599 7260, USA
    Mol Cell Biol 27:721-31. 2007
    ....
  35. pmc Rad6-Bre1-mediated histone H2B ubiquitylation modulates the formation of double-strand breaks during meiosis
    Kentaro Yamashita
    Department of Biology, Graduate School of Science, and Institute for Protein Research, Osaka University, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 101:11380-5. 2004
    An E2 ubiquitin-conjugating enzyme, Rad6, working with an E3 ubiquitin ligase Bre1, catalyzes monoubiquitylation of histone H2B on a C-terminal lysine residue. The rad6 mutant of Saccharomyces cerevisiae shows a meiotic prophase arrest...
  36. pmc Regulation of gross chromosomal rearrangements by ubiquitin and SUMO ligases in Saccharomyces cerevisiae
    Akira Motegi
    Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Building 49, Room 4A22, Bethesda, MD 20892, USA
    Mol Cell Biol 26:1424-33. 2006
    ..In contrast, Siz1 SUMO ligase and another ubiquitin ligase, Bre1, which target for PCNA and histone H2B, respectively, have GCR-supporting activities...
  37. ncbi Genome-wide nuclear morphology screen identifies novel genes involved in nuclear architecture and gene-silencing in Saccharomyces cerevisiae
    Maria Teresa Teixeira
    Département de Structure et Dynamique des Génomes, Unité de Génétique Moléculaire des Levures, URA 2171 CNRS and UFR 927 Univ P M Curie, Institut Pasteur, 25 rue du Docteur Roux, 75724 Cedex 15, Paris, France
    J Mol Biol 321:551-61. 2002
    ..Interestingly, these nuclear morphology alterations were associated with chromatin-silencing defects. These genes provide a molecular context to explore the functional link between nuclear architecture and gene silencing...

Research Grants1

  1. Repair Pathways for Ionizing Radiation Damage Yeast
    John Game; Fiscal Year: 2006
    ..Recent and existing mutants will be studied in detail with this system to determine the molecular involvement of each gene in DSB repair, sister chromatid exchange and recombination between chromosomes. ..