BEM1

Summary

Gene Symbol: BEM1
Description: phosphatidylinositol-3-phosphate-binding protein BEM1
Alias: SRO1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae
    A Bender
    Department of Biology, University of Michigan, Ann Arbor 48109
    Mol Cell Biol 11:1295-305. 1991
  2. pmc A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization
    Anne Christine Butty
    Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, CH 1066 Epalinges VD, Switzerland
    EMBO J 21:1565-76. 2002
  3. pmc Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud
    J E Adamo
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 155:581-92. 2001
  4. pmc Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle
    Xiang Dong Gao
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Mol Biol Cell 18:2542-60. 2007
  5. ncbi The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating
    A C Butty
    Swiss Institute for Experimental Cancer Research ISREC, Chemin des Boveresses 155, 1066 Epalinges VD, Switzerland
    Science 282:1511-6. 1998
  6. ncbi A GTP-exchange factor required for cell orientation
    A Nern
    Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 391:195-8. 1998
  7. pmc Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast
    J L Brown
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 11:2972-82. 1997
  8. pmc Two active states of the Ras-related Bud1/Rsr1 protein bind to different effectors to determine yeast cell polarity
    H O Park
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 0448, USA
    Proc Natl Acad Sci U S A 94:4463-8. 1997
  9. pmc Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1
    Y Matsui
    Department of Biology, University of Tokyo, Japan
    Mol Cell Biol 12:5690-9. 1992
  10. pmc The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae
    D M Lyons
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 16:4095-106. 1996

Research Grants

Scientific Experts

  • Hay Oak Park
  • Takashi Ito
  • Kirsten Hertveldt
  • Thomas Höfken
  • R Li
  • L J Oehlen
  • Maryna Gorelik
  • Chun Chen Kuo
  • Daniel J Lew
  • Trevin R Zyla
  • Kenji Ogura
  • Konomi Fujimura-Kamada
  • Xiang Dong Gao
  • Benjamin Woods
  • Liedewij Laan
  • Chi Fang Wu
  • Keiichiro Tanaka
  • Dongmei Liu
  • Franz Meitinger
  • Henriette Aksnes
  • Yuan Liao
  • Smita Amarnath
  • Alan R Davidson
  • Matthew J Winters
  • Kaushlendra Tripathi
  • Tomoyuki Takaku
  • Erfei Bi
  • Matthias Peter
  • Fuyuhiko Inagaki
  • Hiroyuki Kumeta
  • Daniel C Williams
  • Lukasz Kozubowski
  • Peter J Novick
  • Kazuma Tanaka
  • Christopher Tiedje
  • Yoshihiro Yamaguchi
  • Derek McCusker
  • A Nern
  • J J Moskow
  • D J Lew
  • A S Gladfelter
  • Y Ellen France
  • Ruben C Aguilar
  • Hao Xu
  • Allison Zajac
  • Cleslei F Zanelli
  • Yunmei Wang
  • Charles Boone
  • Audrey van Drogen-Petit
  • Yukiko Shimada
  • Pamela G Fitch
  • M P Gulli
  • Peter M Pryciak
  • Catherine Zwahlen
  • Ryosuke Kawasaki
  • John H Koschwanez
  • M Peter
  • Andrew W Murray
  • Saravanan Palani
  • Hsin Chen
  • Kazuo Tatebayashi
  • Amparo Andrés-Pons
  • Natasha S Savage
  • Katsuyoshi Yamamoto
  • Gislene Pereira
  • Hui Yu Yang
  • Paul Jorgensen
  • Akiko Nishimura
  • Gilles Charvin
  • Anton Khmelinskii
  • Rachel E Lamson
  • Bahtiyar Kurtulmus
  • Olivier Roumanie
  • Anne Christine Butty
  • Michael Knop
  • Sandrine Morlot
  • Peter Novick
  • Birgit Hub
  • Haruo Saito
  • Fei He
  • Ting Gong
  • Thomas Arnesen
  • Camilla Osberg
  • A Bender
  • Vidyanand Nanjundiah
  • Trupti Kawli
  • Smita Mohanty
  • Y Matsui
  • J W Yu
  • I Bose

Detail Information

Publications63

  1. pmc Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae
    A Bender
    Department of Biology, University of Michigan, Ann Arbor 48109
    Mol Cell Biol 11:1295-305. 1991
    ..The new mutations defined two genes, BEM1 and BEM2; both the bem1 and bem2 mutations are temperature sensitive and are only partially suppressed by MSB1...
  2. pmc A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization
    Anne Christine Butty
    Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, CH 1066 Epalinges VD, Switzerland
    EMBO J 21:1565-76. 2002
    ..The adaptor protein Bem1 localizes to sites of polarized growth where it interacts with Cdc42, Cdc24 and the PAK-like kinase Cla4...
  3. pmc Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud
    J E Adamo
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 155:581-92. 2001
    ..Rather, we suggest that Cdc42 acts as an allosteric regulator of the vesicle docking and fusion apparatus to provide maximal function at sites of polarized growth...
  4. pmc Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle
    Xiang Dong Gao
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Mol Biol Cell 18:2542-60. 2007
    ..Together, these results suggest that Axl2p plays sequential and distinct roles in the regulation of cellular morphogenesis in yeast cell cycle...
  5. ncbi The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating
    A C Butty
    Swiss Institute for Experimental Cancer Research ISREC, Chemin des Boveresses 155, 1066 Epalinges VD, Switzerland
    Science 282:1511-6. 1998
    ..Thus, Far1p functions as an adaptor that recruits polarity establishment proteins to the site of extracellular signaling marked by Gbetagamma to polarize assembly of the cytoskeleton in a morphogenetic gradient...
  6. ncbi A GTP-exchange factor required for cell orientation
    A Nern
    Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 391:195-8. 1998
    ..Our results demonstrate that the association of an exchange factor and the betagamma subunit of a hetero-trimeric G protein links receptor-mediated activation to oriented cell growth...
  7. pmc Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast
    J L Brown
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 11:2972-82. 1997
    ..Thus, Gic1p and Gic2p define a novel class of Cdc42p targets that are specifically required for cytoskeletal polarization in vivo...
  8. pmc Two active states of the Ras-related Bud1/Rsr1 protein bind to different effectors to determine yeast cell polarity
    H O Park
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 0448, USA
    Proc Natl Acad Sci U S A 94:4463-8. 1997
    ..group of proteins, which includes a Rho-like GTPase (Cdc42), its guanine nucleotide exchange factor (Cdc24), and Bem1, is necessary for organization of the actin cytoskeleton and for cell polarization...
  9. pmc Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1
    Y Matsui
    Department of Biology, University of Tokyo, Japan
    Mol Cell Biol 12:5690-9. 1992
    ..Two of them were identical with CDC42 and BEM1, bud site assembly genes involved in the process of bud emergence...
  10. pmc The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae
    D M Lyons
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 16:4095-106. 1996
    ..b>Bem1 localizes near the cortical actin cytoskeleton and is essential for polarized growth during mating...
  11. pmc Yeast src homology region 3 domain-binding proteins involved in bud formation
    Y Matsui
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan
    J Cell Biol 133:865-78. 1996
    ..involved in the maintenance of cell polarity for bud formation, and the rho3 defect is suppressed by a high dose of BEM1. Mutational analysis revealed that the second SH3 domain from the NH2 terminus (SH3-2) of Bem1p is important for ..
  12. pmc Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast
    J Peterson
    Department of Biology, Indiana University, Bloomington 47405
    J Cell Biol 127:1395-406. 1994
    ..interact with Bem1p, we screened for mutations that display synthetic lethality with a mutant allele of the BEM1 gene and for genes whose products display two-hybrid interactions with the Bem1 protein...
  13. ncbi Pheromone response in yeast: association of Bem1p with proteins of the MAP kinase cascade and actin
    T Leeuw
    Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    Science 270:1210-3. 1995
    ..Thus, the association of Bem1p with Ste20p and Ste5p may contribute to the conveyance of spatial information that regulates polarized rearrangement of the actin cytoskeleton during yeast mating...
  14. pmc Identification of the bud emergence gene BEM4 and its interactions with rho-type GTPases in Saccharomyces cerevisiae
    D Mack
    Department of Biology, Indiana University, Bloomington 47405, USA
    Mol Cell Biol 16:4387-95. 1996
    ..Models for the role of Bem4p include that it serves as a chaperone or modulates the interaction of these GTPases with one or more of their targets or regulators...
  15. ncbi A novel Cdc42-interacting domain of the yeast polarity establishment protein Bem1. Implications for modulation of mating pheromone signaling
    Yoshihiro Yamaguchi
    Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa 277 8561, Japan
    J Biol Chem 282:29-38. 2007
    ..A multidomain protein Bem1 interacts not only with Cdc42 but also with Cdc24 and the effectors of Cdc42, including the p21-activated kinase ..
  16. pmc High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A
    K R Ayscough
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3202, USA
    J Cell Biol 137:399-416. 1997
    ..Finally, transient actin depolymerization caused many cells to abandon one bud site or mating projection and to initiate growth at a second site. Thus, actin filaments are also required for maintenance of an axis of cell polarity...
  17. pmc Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast
    L Bender
    Department of Biology, Indiana University, Bloomington 47405, USA
    J Cell Biol 133:879-94. 1996
    ....
  18. ncbi Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p
    I Bose
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 276:7176-86. 2001
    ..We suggest that Bem1p acts to concentrate polarity establishment proteins at a discrete site, facilitating polarization and promoting Cdc24p phosphorylation at specific times during the cell cycle...
  19. ncbi Interactions among proteins involved in bud-site selection and bud-site assembly in Saccharomyces cerevisiae
    Y Zheng
    Department of Pharmacology, Cornell University, Ithaca, New York 14850
    J Biol Chem 270:626-30. 1995
    ..The SH3 domain-containing bud-site assembly protein Bem1 also binds directly to Cdc24, and we show here that this interaction is inhibited by Ca2+...
  20. pmc Isolation and characterization of effector-loop mutants of CDC42 in yeast
    A S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Biol Cell 12:1239-55. 2001
    ..The availability of partial function alleles of CDC42 in a genetically tractable system serves as a useful starting point for genetic approaches to identify such novel effectors...
  21. pmc Inhibitory GEF phosphorylation provides negative feedback in the yeast polarity circuit
    Chun Chen Kuo
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 24:753-9. 2014
    ..These findings reveal a mechanism for negative feedback and suggest that the function of negative feedback via GEF inhibition is to buffer the level of Cdc42 at the polarity site...
  22. pmc Polarity establishment requires localized activation of Cdc42
    Benjamin Woods
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710
    J Cell Biol 211:19-26. 2015
    ..Here we show that Cdc42 activation must be localized for successful polarity establishment, supporting local activation rather than local delivery as the dominant mechanism in this system...
  23. pmc N-terminal acetylation by NatC is not a general determinant for substrate subcellular localization in Saccharomyces cerevisiae
    Henriette Aksnes
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS ONE 8:e61012. 2013
    ..Furthermore, all organelle-localized substrates indicated undisrupted structures, thus suggesting that absence of NatC acetylation does not have a vast effect on organelle morphology in yeast...
  24. doi Yeast osmosensors Hkr1 and Msb2 activate the Hog1 MAPK cascade by different mechanisms
    Keiichiro Tanaka
    1Institute of Medical Science, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Sci Signal 7:ra21. 2014
    ..Specifically, activation of Hog1 by Msb2 required the scaffold protein Bem1 and the actin cytoskeleton...
  25. pmc Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p
    Dongmei Liu
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92130
    J Cell Biol 207:59-72. 2014
    ..Mutations in Exo70p that disrupt its interaction with Bem1, without impairing its interactions with other known binding partners, lead to the loss of actin-independent ..
  26. doi A memory system of negative polarity cues prevents replicative aging
    Franz Meitinger
    Molecular Biology of Centrosomes and Cilia, German Cancer Research Center DKFZ, DKFZ ZMBH Alliance, Im Neuenheimer Feld 581, Heidelberg 69120, Germany
    Cell 159:1056-69. 2014
    ..Our work thus established CRMs as negative polarity cues that prevent Cdc42 reactivation to sustain the fitness of replicating cells. ..
  27. pmc Evolutionary adaptation after crippling cell polarization follows reproducible trajectories
    Liedewij Laan
    FAS Center for Systems Biology, Department of Molecular and Cellular Biology, Harvard University, Cambridge, United States
    elife 4:. 2015
    ..We evolved Saccharomyces cerevisiae for 1000 generations without the important polarity gene BEM1. Initially the bem1∆ lineages rapidly increase in fitness and then slowly reach >90% of the fitness of their ..
  28. pmc Association of the cell cycle transcription factor Mbp1 with the Skn7 response regulator in budding yeast
    N Bouquin
    Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Mol Biol Cell 10:3389-400. 1999
    ..Thus, Skn7 and Mbp1 seem to form a transcription factor independent of MBF. Genetic data suggest that this new transcription factor could be involved in the bud-emergence process...
  29. pmc Msb1 interacts with Cdc42, Boi1, and Boi2 and may coordinate Cdc42 and Rho1 functions during early stage of bud development in budding yeast
    Yuan Liao
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China
    PLoS ONE 8:e66321. 2013
    ..cdc24 and cdc42 mutants at restrictive temperature, while deletion of MSB1 showed synthetic lethality with cdc24, bem1, and bem2 mutations. However, the mechanism of how Msb1 regulates Cdc42 function remains poorly understood...
  30. pmc Pleiotropic roles of a ribosomal protein in Dictyostelium discoideum
    Smita Amarnath
    Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India
    PLoS ONE 7:e30644. 2012
    ..cDNA that encodes the ribosomal protein S4 (DdS4) rescues mutations in the cell cycle genes cdc24, cdc42 and bem1. The products of these genes affect morphogenesis in yeast via a coordinated moulding of the cytoskeleton during ..
  31. pmc Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface
    Maryna Gorelik
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    J Biol Chem 287:9168-77. 2012
    ..Remarkably, this modulation of local binding energetics can explain the distinct and highly nuanced binding specificities of these two domains...
  32. pmc Essential role of the NH2-terminal region of Cdc24 guanine nucleotide exchange factor in its initial polarized localization in Saccharomyces cerevisiae
    Konomi Fujimura-Kamada
    Division of Molecular Interaction, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan
    Eukaryot Cell 11:2-15. 2012
    ..These Cdc24-ts mutant proteins did not interact with Bem1 at the COOH-terminal PB1 domain, suggesting a lack of exposure of the PB1 domain in the mutant proteins...
  33. pmc Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex
    Lukasz Kozubowski
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 18:1719-26. 2008
    ..Cdc42p concentrates at a random cortical site during symmetry breaking in a manner that requires the scaffold protein Bem1p. The mechanism whereby Bem1p promotes this polarization was unknown...
  34. ncbi Proteins involved in sterol synthesis interact with Ste20 and regulate cell polarity
    Christopher Tiedje
    Institute of Biochemistry, Christian Albrecht University Kiel, Olshausenstrasse 40, 24098 Kiel, Germany
    J Cell Sci 120:3613-24. 2007
    ..Lack of CBR1 produced no detectable phenotype, whereas the deletion of CBR1 in the absence of NCP1 was lethal. Using a conditional lethal mutant we demonstrate that both proteins have overlapping functions in bud morphology...
  35. pmc Analysis of SEC9 suppression reveals a relationship of SNARE function to cell physiology
    Daniel C Williams
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA
    PLoS ONE 4:e5449. 2009
    ..Although fusion of secretory vesicles with the plasma membrane is important for yeast cell growth, the relationship between exocytic SNAREs and cell physiology has not been established...
  36. doi NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae
    Kenji Ogura
    Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita ku, Sapporo 060 0812, Japan
    J Biochem 146:317-25. 2009
    b>Bem1 and Cdc24 of the budding yeast Saccharomyces cerevisiae interact with each other through PB1-PB1 heterodimer formation to regulate the establishment of cell polarity...
  37. pmc Solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42
    Tomoyuki Takaku
    Laboratory of Structural Biology, Graduate School of Life Science, Hokkaido University, N 12, W 6 Kita ku, Sapporo 060 0812, Japan
    J Biol Chem 285:19346-53. 2010
    b>Bem1 is a scaffold protein essential for the establishment of cell polarity in Saccharomyces cerevisiae...
  38. pmc A Conserved residue in the yeast Bem1p SH3 domain maintains the high level of binding specificity required for function
    Maryna Gorelik
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    J Biol Chem 286:19470-7. 2011
    ..This work provides a clear illustration of the importance of intrinsic binding specificity for the function of protein-protein interaction modules, and the key role of "negative" interactions in determining the specificity of a domain...
  39. pmc Cellular morphogenesis under stress is influenced by the sphingolipid pathway gene ISC1 and DNA integrity checkpoint genes in Saccharomyces cerevisiae
    Kaushlendra Tripathi
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Genetics 189:533-47. 2011
    ..Mechanistically, the checkpoint regulator Rad53 partially influences isc1Δ cell morphology in a dosage-dependent manner...
  40. pmc Novel modular domain PB1 recognizes PC motif to mediate functional protein-protein interactions
    T Ito
    Division of Genome Biology, Cancer Research Institute, Kanazawa University, 13 1 Takaramachi, Kanazawa 920 0934, Japan
    EMBO J 20:3938-46. 2001
    ..This domain-swapping experiment demonstrates that Bem1p function requires interaction with Cdc24p, in which the PB1 domain and the PC motif participate as responsible modules...
  41. ncbi Phosphorylation of the Cdc42 exchange factor Cdc24 by the PAK-like kinase Cla4 may regulate polarized growth in yeast
    M P Gulli
    Swiss Institute for Experimental Cancer Research Chemin des Boveresses 155 1066 Epalinges, Vaud, Switzerland
    Mol Cell 6:1155-67. 2000
    ..GEF for Cdc42, from the nucleus to the polarization site, where it is stably maintained by binding to the adaptor Bem1. Locally activated Cdc42 then polarizes the cytoskeleton in a manner dependent on its effectors Bni1 and the PAK-..
  42. pmc A Cdc24p-Far1p-Gbetagamma protein complex required for yeast orientation during mating
    A Nern
    Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, United Kingdom
    J Cell Biol 144:1187-202. 1999
    ..These results suggest that formation of a Cdc24p-Far1p-Gbetagamma complex functions as a landmark for orientation of the cytoskeleton during growth towards an external signal...
  43. ncbi Potential regulation of Ste20 function by the Cln1-Cdc28 and Cln2-Cdc28 cyclin-dependent protein kinases
    L J Oehlen
    Rockefeller University, New York, New York 10021, USA
    J Biol Chem 273:25089-97. 1998
    ....
  44. pmc Bee1, a yeast protein with homology to Wiscott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton
    R Li
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 136:649-58. 1997
    ..Thus, Bee1 protein may be a crucial component of a cytoskeletal complex that controls the assembly and organization of actin filaments at the cell cortex...
  45. ncbi Genetic interactions indicate a role for Mdg1p and the SH3 domain protein Bem1p in linking the G-protein mediated yeast pheromone signalling pathway to regulators of cell polarity
    E Leberer
    Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada
    Mol Gen Genet 252:608-21. 1996
    ..The same genetic screen identified BEM1, which encodes an SH3 domain protein required for polarized morphogenesis in response to pheromone, and a novel ..
  46. pmc Interaction with the SH3 domain protein Bem1 regulates signaling by the Saccharomyces cerevisiae p21-activated kinase Ste20
    Matthew J Winters
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 377 Plantation St, Four Biotech, Rm 330, Worcester, MA 01605, USA
    Mol Cell Biol 25:2177-90. 2005
    ..Another factor that functions with Ste20 and Cdc42 is the protein Bem1. Bem1 has two SH3 domains, but target ligands for these domains have not been described...
  47. pmc High-resolution genetic mapping with ordered arrays of Saccharomyces cerevisiae deletion mutants
    Paul Jorgensen
    Department of Medical Genetics and Microbiology, University of Toronto, Ontario M5S 1A8, Canada
    Genetics 162:1091-9. 2002
    ..In principle, SGAM should be applicable to the analysis of multigenic traits. Large-scale construction of ordered mutations in other model organisms would broaden the application of this approach...
  48. ncbi The upstream regulator, Rsr1p, and downstream effectors, Gic1p and Gic2p, of the Cdc42p small GTPase coordinately regulate initiation of budding in Saccharomyces cerevisiae
    Ryosuke Kawasaki
    Division of Molecular Interaction, Institute for Genetic Medicine, Hokkaido University Graduate School of Medicine, N15 W7, Kita ku, Sapporo, 060 0815, Japan
    Genes Cells 8:235-50. 2003
    ..However, this model only addresses how Rsr1p determines the budding site, because the rsr1 mutant normally initiates budding...
  49. pmc Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2
    Thomas Höfken
    The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Rd, Manchester, M20 4BX, UK
    J Cell Biol 164:219-31. 2004
    ..Moreover, Gic1 bound directly to Bub2 and prevented binding of the GTPase Tem1 to Bub2. We propose that in anaphase the Cdc42-regulated Gic proteins trigger mitotic exit by interfering with Bfa1-Bub2 GTPase-activating protein function...
  50. pmc The nucleotide exchange factor Cdc24p may be regulated by auto-inhibition
    Yukiko Shimada
    Swiss Institute for Experimental Cancer Research ISREC, Epalinges VD, Switzerland
    EMBO J 23:1051-62. 2004
    ..Taken together, our results support a two-step molecular mechanism for the site-specific activation of Cdc24p, which involves Rsr1p/Bud1p and the adaptor protein Bem1p...
  51. ncbi Insight into molecular interactions between two PB1 domains
    Audrey van Drogen-Petit
    Institute of Chemistry, Swiss Federal Institut of Technology Lausanne EPFL, Switzerland
    J Mol Biol 336:1195-210. 2004
    ..Recently, a new protein-protein interaction domain termed PB1 (Phox and Bem1) was identified, which is conserved throughout evolution and present in diverse proteins functioning in signal ..
  52. pmc Lrg1p Is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast
    Pamela G Fitch
    The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
    Genetics 168:733-46. 2004
    ..Higher dosage of three genes, BEM1, LRG1, and FUS1, partially suppressed the fus2Delta cell fusion defect...
  53. pmc Cyclical regulation of the exocyst and cell polarity determinants for polarized cell growth
    Allison Zajac
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Mol Biol Cell 16:1500-12. 2005
    ..We propose that a cyclical regulatory network contributes to the establishment and maintenance of polarized cell growth in yeast...
  54. ncbi Cdc24 regulates nuclear shuttling and recruitment of the Ste5 scaffold to a heterotrimeric G protein in Saccharomyces cerevisiae
    Yunmei Wang
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:13084-96. 2005
    ..Collectively, these findings suggest that Cdc24 mediates site-specific localization of Ste5 to a heterotrimeric G protein and may therefore ensure localized activation of the associated MAPK cascade...
  55. pmc Cdc42 regulation of kinase activity and signaling by the yeast p21-activated kinase Ste20
    Rachel E Lamson
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell Biol 22:2939-51. 2002
    ..In total, our observations indicate that Cdc42 converts Ste20 to an active form, while pathway stimuli regulate the ability of this active Ste20 to trigger signaling through a particular pathway...
  56. pmc Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant
    Cleslei F Zanelli
    Department of Biological Sciences, School of Pharmaceutical Sciences, Sao Paulo State University, UNESP, Rodovia Araraquara Jaú, km 01, Araraquara, São Paulo 14801 902, Brazil
    Genetics 171:1571-81. 2005
    ..Taken together, these data strongly support the correlated involvement of Pkc1 and eIF5A in establishing actin polarity, which is essential for bud formation and G1/S transition in S. cerevisiae...
  57. ncbi The polarity-establishment component Bem1p interacts with the exocyst complex through the Sec15p subunit
    Y Ellen France
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    J Cell Sci 119:876-88. 2006
    ..This, in turn, helps to coordinate the secretory pathway and polarized bud growth...
  58. pmc Epsin N-terminal homology domains perform an essential function regulating Cdc42 through binding Cdc42 GTPase-activating proteins
    Ruben C Aguilar
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 103:4116-21. 2006
    ..Furthermore, the epsins contribute to regulation of specific Cdc42 signaling pathways in yeast cells. These data support a model in which the epsins function as spatial and temporal coordinators of endocytosis and cell polarity...
  59. ncbi Whole genome phage display selects for proline-rich Boi polypeptides against Bem1p
    Kirsten Hertveldt
    Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven B 3001, Belgium
    Biotechnol Lett 28:1233-9. 2006
    ..Target Bem1p was a doubly-tagged recombinant, Bem1([Asn142-Ile551]), which strongly interacts in ELISA with a C-terminal 75 amino acids polypeptide from Cdc24p ..
  60. ncbi Bem1p is a positive regulator of the homotypic fusion of yeast vacuoles
    Hao Xu
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    J Biol Chem 281:27158-66. 2006
    ..from bem1Delta strains showed a strong reduction in the rate of lipid mixing when compared with vacuoles from the BEM1 parent...
  61. ncbi Functional interactions between the VRP1-LAS17 and RHO3-RHO4 genes involved in actin cytoskeleton organization in Saccharomyces cerevisiae
    Olivier Roumanie
    Laboratoire de Biologie Moléculaire et Séquençage, IBGC, UMR Université Victor Segalen Bordeaux 2 CNRS 5095, 146 rue Leo Saignat, BP 64, 33076 Bordeaux, France
    Curr Genet 40:317-25. 2002
    ..In addition, other observations lead us to propose that Rvs167p may act as a linking protein between the two cellular elements...
  62. ncbi Cdk1 coordinates cell-surface growth with the cell cycle
    Derek McCusker
    Department of Molecular, Cell and Developmental Biology, University of California, 1156 High Street, Santa Cruz, CA 95064, USA
    Nat Cell Biol 9:506-15. 2007
    ..A mutant form of a Cdc24-associated protein that fails to undergo Cdk1-dependent phosphorylation causes defects in bud growth. These results provide a direct link between Cdk1 activity and the control of polarized cell growth...
  63. ncbi All phox homology (PX) domains from Saccharomyces cerevisiae specifically recognize phosphatidylinositol 3-phosphate
    J W Yu
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6059, USA
    J Biol Chem 276:44179-84. 2001
    ..Our results establish that PtdIns-3-P, and not other phosphoinositides, is the target of all PX domains in S. cerevisiae and suggest a role for PX domains in assembly of multiprotein complexes at specific membrane surfaces...

Research Grants2

  1. SPATIAL CONTROL OF CELL POLARITY DURING YEAST BUDDING
    Hay Oak Park; Fiscal Year: 2003
    ..The second group of proteins includes the Rho-like GTPase Cdc42, Cdc24, and Bem1. It is believed that the spatial control of budding and actin polarization involves a cascade of GTPases, Bud1 ..
  2. Regulation of polarized cell growth by GTPases
    Hay Oak Park; Fiscal Year: 2009
    ..abstract_text> ..