ARX1

Summary

Gene Symbol: ARX1
Description: putative hydrolase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc The putative GTPases Nog1p and Lsg1p are required for 60S ribosomal subunit biogenesis and are localized to the nucleus and cytoplasm, respectively
    George Kallstrom
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA
    Mol Cell Biol 23:4344-55. 2003
  2. pmc Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation
    Lisa Kappel
    Institut für Molekulare Biowissenschaften, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    J Cell Biol 199:771-82. 2012
  3. pmc Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes
    Marius B Faza
    Institute of Biochemistry IBC, ETH Zurich, Zurich, Switzerland
    PLoS Genet 8:e1002915. 2012
  4. pmc Integrity of the P-site is probed during maturation of the 60S ribosomal subunit
    Cyril Bussiere
    Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    J Cell Biol 197:747-59. 2012
  5. pmc The mRNA export factor Npl3 mediates the nuclear export of large ribosomal subunits
    Alexandra Hackmann
    Abteilung für Molekulare Genetik, Institut fur Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften, Georg August Universitat Gottingen, Grisebachstrasse 8, Göttingen 37077, Germany
    EMBO Rep 12:1024-31. 2011
  6. pmc Defining the pathway of cytoplasmic maturation of the 60S ribosomal subunit
    Kai Yin Lo
    Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Cell 39:196-208. 2010
  7. pmc Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export
    Yanhua Yao
    Institut Pasteur, Unité de Génétique des Interactions Macromoléculaires, CNRS URA2171, F 75724 Paris Cedex 15, France
    RNA 16:1007-17. 2010
  8. pmc The cytosolic J-protein, Jjj1, and Rei1 function in the removal of the pre-60 S subunit factor Arx1
    Alison E Meyer
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 285:961-8. 2010
  9. pmc Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation
    K Mark Parnell
    Department of Biochemistry and Howard Hughes Medical Institute, University of Utah School of Medicine, 15 N Medical Drive East, Room 4800, Salt Lake City, UT 84112 5650, USA
    Mol Cell Biol 29:4014-23. 2009
  10. pmc Arx1 is a nuclear export receptor for the 60S ribosomal subunit in yeast
    Nai Jung Hung
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Biol Cell 19:735-44. 2008

Scientific Experts

  • K Mark Parnell
  • Arlen W Johnson
  • Kai Yin Lo
  • Nai Jung Hung
  • Alice Lebreton
  • Lisa Kappel
  • Brigitte Pertschy
  • Alison E Meyer
  • Cosmin Saveanu
  • Alain Jacquier
  • Micheline Fromont-Racine
  • Mathias Loibl
  • Laura Occhipinti
  • Ed Hurt
  • Cyril Bussiere
  • Helmut Bergler
  • Gertrude Zisser
  • Chengying Ma
  • Ya Han Ting
  • Anshuk Sarkar
  • Basil Johannes Greber
  • Matthias Thoms
  • Yoshitaka Matsuo
  • Daniel Boehringer
  • Nenad Ban
  • Christopher D Castle
  • Isabella Klein
  • Dagmar Pratte
  • Vikram G Panse
  • Yiming Chang
  • Stefan Kemmler
  • Dieter Kressler
  • Antonio Fernández-Pevida
  • Marius B Faza
  • Basil J Greber
  • Alexandra Hackmann
  • Yanhua Yao
  • Juliane Merl
  • Véronique Albanèse
  • Pascal Lenormand
  • Elizabeth A Craig
  • Wei Yao
  • Suresh Kumar S
  • Ting Jun Lu
  • Lvqin Zheng
  • Bo Ru Chen
  • Jianlin Lei
  • Ning Gao
  • Kaige Yan
  • Yan Chen
  • Zhifei Li
  • Ningning Li
  • Jing Ting Shie
  • John L Woolford
  • Shan Wu
  • Roland Beckmann
  • Markus Pech
  • Ruedi Aebersold
  • Marc Leibundgut
  • Vikram Govind Panse
  • Stefan Gerhardy
  • George Kallstrom
  • Michèle Salem
  • Alexander Leitner
  • Nicolas Leulliot
  • Anna Gungl
  • Michael Prattes
  • Rizos Georgios Manikas
  • Elmar Krieger
  • Claudia Schmidt
  • David Tollervey
  • Sander Granneman
  • Victoria Borgianini
  • Varada Dandekar
  • Guillaume Murat
  • Ujjwala Singh
  • Anna M Menet
  • Martin Altvater
  • Catherine Denicourt
  • Richa Sardana
  • J Bassler
  • Christof Gruber
  • Sucheta Arora
  • Jesus de la Cruz
  • Gernot Fruhmann
  • Gerald Rechberger
  • Stefan Unterweger
  • Antonio Díaz-Quintana
  • Christian Montellese
  • Joachim Frank

Detail Information

Publications31

  1. pmc The putative GTPases Nog1p and Lsg1p are required for 60S ribosomal subunit biogenesis and are localized to the nucleus and cytoplasm, respectively
    George Kallstrom
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA
    Mol Cell Biol 23:4344-55. 2003
    ..Since Lsg1p is cytoplasmic, its role in nuclear export is likely to be indirect. We suggest that Lsg1p is needed to recycle an export factor(s) that shuttles from the nucleus associated with the nascent 60S subunit...
  2. pmc Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation
    Lisa Kappel
    Institut für Molekulare Biowissenschaften, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    J Cell Biol 199:771-82. 2012
    ..Our results show that release of Rlp24 by Drg1 defines a key event in large subunit formation that is a prerequisite for progression of cytoplasmic pre-60S maturation...
  3. pmc Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes
    Marius B Faza
    Institute of Biochemistry IBC, ETH Zurich, Zurich, Switzerland
    PLoS Genet 8:e1002915. 2012
    ..Mex67-Mtr2 could engage a regulatory crosstalk among the three major export pathways for optimal cellular growth and proliferation...
  4. pmc Integrity of the P-site is probed during maturation of the 60S ribosomal subunit
    Cyril Bussiere
    Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    J Cell Biol 197:747-59. 2012
    ..We propose that Efl1 promotes a functional check of the integrity of the 60S subunit before its first round of translation...
  5. pmc The mRNA export factor Npl3 mediates the nuclear export of large ribosomal subunits
    Alexandra Hackmann
    Abteilung für Molekulare Genetik, Institut fur Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften, Georg August Universitat Gottingen, Grisebachstrasse 8, Göttingen 37077, Germany
    EMBO Rep 12:1024-31. 2011
    ..Mutations in NPL3 lead to export defects of the large subunit and genetic interactions with other pre-60S export factors...
  6. pmc Defining the pathway of cytoplasmic maturation of the 60S ribosomal subunit
    Kai Yin Lo
    Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Cell 39:196-208. 2010
    ..Finally, the release of Tif6 is a prerequisite for the release of the nuclear export adaptor Nmd3. Establishing this pathway provides an important conceptual framework for understanding ribosome maturation...
  7. pmc Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export
    Yanhua Yao
    Institut Pasteur, Unité de Génétique des Interactions Macromoléculaires, CNRS URA2171, F 75724 Paris Cedex 15, France
    RNA 16:1007-17. 2010
    ..Here, we report the identification of a novel pre-60S factor, Ecm1, which partially acts like Arx1 and becomes essential when the NPC function is affected...
  8. pmc The cytosolic J-protein, Jjj1, and Rei1 function in the removal of the pre-60 S subunit factor Arx1
    Alison E Meyer
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 285:961-8. 2010
    ..One cytosolic step has two components: 1) the removal of the maturation factor Arx1, which transits from the nucleus to the cytosol with the pre-60 S subunit, and 2) its subsequent transport back ..
  9. pmc Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation
    K Mark Parnell
    Department of Biochemistry and Howard Hughes Medical Institute, University of Utah School of Medicine, 15 N Medical Drive East, Room 4800, Salt Lake City, UT 84112 5650, USA
    Mol Cell Biol 29:4014-23. 2009
    ..found in all eukaryotes, was previously shown to be necessary for the nuclear reimport of 60S subunit export factor Arx1. In this study we investigate the function of Reh1, a protein with high sequence similarity to Rei1...
  10. pmc Arx1 is a nuclear export receptor for the 60S ribosomal subunit in yeast
    Nai Jung Hung
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Biol Cell 19:735-44. 2008
    ..Here, we show that the previously characterized 60S subunit biogenesis factor, Arx1, also acts as an export receptor for the 60S subunit...
  11. pmc Reengineering ribosome export
    Kai Yin Lo
    Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA
    Mol Biol Cell 20:1545-54. 2009
    ..subunit is one of the bulkiest transport cargoes, and in yeast three different receptors, Crm1, Mex67/Mtr2, and Arx1, collaborate in its export...
  12. pmc A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export
    Wei Yao
    Biochemie Zentrum der Universitat Heidelberg, Heidelberg, Germany
    EMBO J 27:6-16. 2008
    ..Thus, the Mex67-Mtr2 export receptor employs a versatile binding platform on its surface that could create a crosstalk between mRNA and ribosome export pathways...
  13. pmc Cytoplasmic recycling of 60S preribosomal factors depends on the AAA protein Drg1
    Brigitte Pertschy
    Institut für Molekulare Biowissenschaften Karl Franzens Universität Graz, Universitatsplatz 2, A 8010, Graz, Austria
    Mol Cell Biol 27:6581-92. 2007
    ..of Drg1 leads to an increased cytoplasmic localization of shuttling pre-60S maturation factors like Rlp24, Arx1, and Tif6...
  14. pmc The specialized cytosolic J-protein, Jjj1, functions in 60S ribosomal subunit biogenesis
    Alison E Meyer
    Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 104:1558-63. 2007
    ....
  15. pmc A functional network involved in the recycling of nucleocytoplasmic pre-60S factors
    Alice Lebreton
    Genetique des Interactions Macromoleculaires, Centre National de la Recherche Scientifique URA2171, Institut Pasteur, 75724 Paris Cedex 15, France
    J Cell Biol 173:349-60. 2006
    ..The accumulation in the cytoplasm of a small complex formed by the association of Arx1 with a novel factor, Alb1/Yjl122w, inhibits the release of the putative antiassociation factor Tif6 from the ..
  16. pmc Nuclear recycling of the pre-60S ribosomal subunit-associated factor Arx1 depends on Rei1 in Saccharomyces cerevisiae
    Nai Jung Hung
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, 1 University Station, A5000, The University of Texas at Austin, Austin, Texas 78712 0162, USA
    Mol Cell Biol 26:3718-27. 2006
    b>Arx1 and Rei1 are found on late pre-60S ribosomal particles containing the export adaptor Nmd3...
  17. ncbi Identification of a 60S preribosomal particle that is closely linked to nuclear export
    J Bassler
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Mol Cell 8:517-29. 2001
    ..Eight of these proteins were copurified with nuclear pore complexes, suggesting that this complex represents the transport intermediate for 60S subunit export...
  18. pmc 60S ribosomal subunit assembly dynamics defined by semi-quantitative mass spectrometry of purified complexes
    Alice Lebreton
    Institut Pasteur, Unité de Génétique des Interactions Macromoléculaires, CNRS URA2171, Paris, France
    Nucleic Acids Res 36:4988-99. 2008
    ..These results led to the characterisation of the composition of early, intermediate and late pre-ribosomal complexes, specific for crucial maturation steps during 60S assembly in eukaryotes...
  19. doi Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1
    Chengying Ma
    Ministry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, China
    Nat Struct Mol Biol . 2017
    ..These findings pinpoint a structural checkpoint role for Nmd3 in PTC assembly, and provide information about functional and mechanistic roles of these assembly factors in the maturation of the 60S ribosomal subunit...
  20. pmc Bcp1 Is the Nuclear Chaperone of Rpl23 in Saccharomyces cerevisiae
    Ya Han Ting
    From the Department of Agricultural Chemistry, National Taiwan University, 1 Sec 4, Roosevelt Road, Taipei 10617, Taiwan
    J Biol Chem 292:585-596. 2017
    ..Loss of Bcp1 causes instability of Rpl23 and deficiency of 60S subunits. In summary, Bcp1 is a novel 60S biogenesis factor via chaperoning Rpl23 in the nucleus...
  21. doi Ribosome-stalk biogenesis is coupled with recruitment of nuclear-export factor to the nascent 60S subunit
    Anshuk Sarkar
    Biochemistry Centre, University of Heidelberg, Heidelberg, Germany
    Nat Struct Mol Biol 23:1074-1082. 2016
    ..Thus, a spatiotemporal mark on the ribosomal stalk controls the recruitment of an RNA-export receptor to the nascent 60S subunit...
  22. doi Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation
    Basil Johannes Greber
    Institute of Molecular Biology and Biophysics, Department of Biology, ETH Zurich, CH 8093 Zurich, Switzerland
    Cell 164:91-102. 2016
    ..cryo-electron microscopy (cryo-EM) structure of the yeast 60S subunit in complex with the biogenesis factors Rei1, Arx1, and Alb1 at 3.4 Å resolution...
  23. pmc The drug diazaborine blocks ribosome biogenesis by inhibiting the AAA-ATPase Drg1
    Mathias Loibl
    From the Institut für Molekulare Biowissenschaften, Karl Franzens Universitat Graz, A 8010 Graz, Austria and
    J Biol Chem 289:3913-22. 2014
    ..Our results identify the first target of an inhibitor of ribosome biogenesis and provide the mechanism of inhibition of a key step in large ribosomal subunit formation. ..
  24. pmc Mak5 and Ebp2 act together on early pre-60S particles and their reduced functionality bypasses the requirement for the essential pre-60S factor Nsa1
    Dagmar Pratte
    Unit of Biochemistry, Department of Biology, University of Fribourg, Fribourg, Switzerland
    PLoS ONE 8:e82741. 2013
    ....
  25. pmc Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export
    Yoshitaka Matsuo
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, Heidelberg D 69120, Germany
    Nature 505:112-6. 2014
    ..We conclude that Nug2 is a regulatory GTPase that monitors pre-60S maturation, with release from its placeholder site linked to recruitment of the nuclear export machinery. ..
  26. pmc Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2
    Laura Occhipinti
    Department of Biology D BIOL, Institute of Biochemistry IBC, ETH Zurich, Schafmattstrasse 18, CH 8093 Zurich, Switzerland and MLS Program, Life Sciences Zurich Graduate School, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Nucleic Acids Res 41:8266-79. 2013
    ..We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel. ..
  27. pmc Las1 interacts with Grc3 polynucleotide kinase and is required for ribosome synthesis in Saccharomyces cerevisiae
    Christopher D Castle
    Department of Integrative Biology and Pharmacology, Graduate School of Biomedical Sciences, The University of Texas Health Science Center, Houston, TX 77030, USA
    Nucleic Acids Res 41:1135-50. 2013
    ..We propose that Las1 and Grc3 function together in a conserved mechanism to modulate rRNA processing and eukaryotic ribosome biogenesis...
  28. doi Cryo-EM structures of Arx1 and maturation factors Rei1 and Jjj1 bound to the 60S ribosomal subunit
    Basil J Greber
    Institute of Molecular Biology and Biophysics, Eidgenossische Technische Hochschule ETH Zurich, Zurich, Switzerland
    Nat Struct Mol Biol 19:1228-33. 2012
    ..We have biochemically characterized ribosomal complexes of the yeast 60S-biogenesis factor Arx1 and late-maturation factors Rei1 and Jjj1 and determined their cryo-EM structures...
  29. pmc Yeast ribosomal protein L40 assembles late into precursor 60 S ribosomes and is required for their cytoplasmic maturation
    Antonio Fernández-Pevida
    Departamento de Genetica, Universidad de Sevilla, E 41012 Sevilla, Spain
    J Biol Chem 287:38390-407. 2012
    ....
  30. pmc A ribosome-anchored chaperone network that facilitates eukaryotic ribosome biogenesis
    Véronique Albanèse
    Department of Biology, Stanford University, Palo Alto, CA 94305, USA
    J Cell Biol 189:69-81. 2010
    ..Our results demonstrate that, in addition to their known cytoplasmic roles in de novo protein folding, some ribosome-anchored CLIPS chaperones play a critical role in nuclear steps of ribosome biogenesis...
  31. pmc Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes
    Juliane Merl
    Institut fur Biochemie, Genetik und Mikrobiologie, University of Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
    Nucleic Acids Res 38:3068-80. 2010
    ..The data presented strengthen the idea that assembly of eukaryotic pre-ribosomal particles can result from transient association of distinct building blocks...