AHA1

Summary

Gene Symbol: AHA1
Description: Aha1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. ncbi Aha1 binds to the middle domain of Hsp90, contributes to client protein activation, and stimulates the ATPase activity of the molecular chaperone
    Gregor P Lotz
    Protein Folding Group, Institute for Genetics, University of Bonn, Romerstrasse 164, D 53117 Bonn, Germany
    J Biol Chem 278:17228-35. 2003
  2. ncbi Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1
    Barry Panaretou
    Division of Life Sciences, Franklin Wilkins Building, 150 Stamford Street, SE1 9NN, London, United Kingdom
    Mol Cell 10:1307-18. 2002
  3. pmc Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery
    Philippe Meyer
    Chester Beatty Laboratories, Section of Structural Biology, The Institute of Cancer Research, London, UK
    EMBO J 23:1402-10. 2004
  4. ncbi Co-chaperone regulation of conformational switching in the Hsp90 ATPase cycle
    Giuliano Siligardi
    Pharmaceutical Optical Spectroscopy Centre and Division of Life Sciences, Department of Pharmacy, King s College London, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NN, United Kingdom
    J Biol Chem 279:51989-98. 2004
  5. pmc Swe1Wee1-dependent tyrosine phosphorylation of Hsp90 regulates distinct facets of chaperone function
    Mehdi Mollapour
    Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Mol Cell 37:333-43. 2010
  6. doi Asymmetric activation of the hsp90 dimer by its cochaperone aha1
    Marco Retzlaff
    Center for Integrated Protein Science Munich at the Department Chemie, Technische Universitat Munchen, D 85747 Garching, Germany
    Mol Cell 37:344-54. 2010
  7. pmc Threonine 22 phosphorylation attenuates Hsp90 interaction with cochaperones and affects its chaperone activity
    Mehdi Mollapour
    Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Mol Cell 41:672-81. 2011
  8. pmc Saccharomyces cerevisiae Tti2 Regulates PIKK Proteins and Stress Response
    Kyle S Hoffman
    Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, N6A5C1 Canada
    G3 (Bethesda) 6:1649-59. 2016
  9. doi Mutation of essential Hsp90 co-chaperones SGT1 or CNS1 renders yeast hypersensitive to overexpression of other co-chaperones
    Jill L Johnson
    Department of Biological Sciences, University of Idaho, P O Box 443051, Moscow, ID, 83844 3051, USA
    Curr Genet 60:265-76. 2014
  10. doi A mutation in the catalytic loop of Hsp90 specifically impairs ATPase stimulation by Aha1p, but not Hch1p
    Natalie K Horvat
    Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    J Mol Biol 426:2379-92. 2014

Scientific Experts

  • Jill L Johnson
  • Mehdi Mollapour
  • Philippe Meyer
  • Jing Li
  • Barry Panaretou
  • Heather Armstrong
  • Laurence H Pearl
  • Chrisostomos Prodromou
  • Peter W Piper
  • Klaus Richter
  • Johannes Buchner
  • Bin Hu
  • Kyle S Hoffman
  • Michael Berg
  • Natalie K Horvat
  • Paul LaPointe
  • Annemarie Wolmarans
  • Rebecca Mercier
  • S Mark Roe
  • Marco Retzlaff
  • Fulai Ran
  • Giuliano Siligardi
  • Chunyan Liao
  • Ignacija Vlasic
  • Cara K Vaughan
  • Gregor P Lotz
  • Jim Karagiannis
  • Christopher J Brandl
  • Julie Genereaux
  • Martin L Duennwald
  • Alexander S McCarton
  • Annette Michalowski
  • Jacob Knowles
  • Leo Spyracopoulos
  • Silke Palzer
  • Kai Sohn
  • Steffen Rupp
  • Brian L Lee
  • Jochen Reinstein
  • Baochan Mai
  • Frederik Gugel
  • Nidhi Gadura
  • Horst Kessler
  • Franz Hagn
  • Lars Mitschke
  • Martin Hessling
  • Corinne A Michels
  • Wolfgang M J Obermann
  • Anja Harst
  • Cara Vaughan
  • Hongying Lin
  • Janis K Sullivan
  • Stefan H Millson
  • Paul Workman
  • Soren Naaby-Hansen
  • Alison Maloney
  • Paul A Clarke
  • Rob Stein
  • Rainer Cramer
  • Shradha Singh

Detail Information

Publications18

  1. ncbi Aha1 binds to the middle domain of Hsp90, contributes to client protein activation, and stimulates the ATPase activity of the molecular chaperone
    Gregor P Lotz
    Protein Folding Group, Institute for Genetics, University of Bonn, Romerstrasse 164, D 53117 Bonn, Germany
    J Biol Chem 278:17228-35. 2003
    ..We have identified Aha1 (activator of Hsp90 ATPase) and its relative Hch1 (high copy Hsp90 suppressor) as binding partners of Hsp90 in ..
  2. ncbi Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1
    Barry Panaretou
    Division of Life Sciences, Franklin Wilkins Building, 150 Stamford Street, SE1 9NN, London, United Kingdom
    Mol Cell 10:1307-18. 2002
    ..A ubiquitous family of stress-regulated proteins have been identified (Aha1, activator of Hsp90 ATPase) that bind directly to Hsp90 and are required for the in vivo Hsp90-dependent activation ..
  3. pmc Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery
    Philippe Meyer
    Chester Beatty Laboratories, Section of Structural Biology, The Institute of Cancer Research, London, UK
    EMBO J 23:1402-10. 2004
    ..The co-chaperones Aha1 and Hch1 bind to Hsp90 and stimulate its ATPase activity...
  4. ncbi Co-chaperone regulation of conformational switching in the Hsp90 ATPase cycle
    Giuliano Siligardi
    Pharmaceutical Optical Spectroscopy Centre and Division of Life Sciences, Department of Pharmacy, King s College London, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NN, United Kingdom
    J Biol Chem 279:51989-98. 2004
    ..isothermal titration calorimetry and difference circular dichroism) and ATPase regulation by the co-chaperones Aha1, Sti1 (Hop), and Sba1 (p23)...
  5. pmc Swe1Wee1-dependent tyrosine phosphorylation of Hsp90 regulates distinct facets of chaperone function
    Mehdi Mollapour
    Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Mol Cell 37:333-43. 2010
    ....
  6. doi Asymmetric activation of the hsp90 dimer by its cochaperone aha1
    Marco Retzlaff
    Center for Integrated Protein Science Munich at the Department Chemie, Technische Universitat Munchen, D 85747 Garching, Germany
    Mol Cell 37:344-54. 2010
    ..dimeric molecular machine regulated by several cochaperones, including inhibitors and the unique ATPase activator Aha1. Here, we analyzed the mechanism of the Aha1-mediated acceleration of Hsp90 ATPase activity and identified the ..
  7. pmc Threonine 22 phosphorylation attenuates Hsp90 interaction with cochaperones and affects its chaperone activity
    Mehdi Mollapour
    Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Mol Cell 41:672-81. 2011
    ..of this residue alters Hsp90 ATPase activity and chaperone function and impacts interaction with the cochaperones Aha1 and Cdc37...
  8. pmc Saccharomyces cerevisiae Tti2 Regulates PIKK Proteins and Stress Response
    Kyle S Hoffman
    Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, N6A5C1 Canada
    G3 (Bethesda) 6:1649-59. 2016
    ..These results indicate that Tti2 does not act as a general chaperone, but may have a specialized function in PIKK folding and/or complex assembly. ..
  9. doi Mutation of essential Hsp90 co-chaperones SGT1 or CNS1 renders yeast hypersensitive to overexpression of other co-chaperones
    Jill L Johnson
    Department of Biological Sciences, University of Idaho, P O Box 443051, Moscow, ID, 83844 3051, USA
    Curr Genet 60:265-76. 2014
    ..However, overexpression of SBA1, PPT1, AHA1 or HCH1 caused varying levels of growth defects in an sgt1-K360E strain...
  10. doi A mutation in the catalytic loop of Hsp90 specifically impairs ATPase stimulation by Aha1p, but not Hch1p
    Natalie K Horvat
    Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    J Mol Biol 426:2379-92. 2014
    ..Our work here suggests that both Hch1p and Aha1p regulate Hsp90 function through interaction with the catalytic loop but do so in different ways. ..
  11. pmc An in vivo photo-cross-linking approach reveals a homodimerization domain of Aha1 in S. cerevisiae
    Michael Berg
    Fraunhofer IGB, Stuttgart, Germany
    PLoS ONE 9:e89436. 2014
    ..photoreactive non-canonical amino acid pAzpa was site-specifically incorporated at eight positions into a domain of Aha1 that was previously described to bind Hsp90 in vitro to function as a cochaperone of Hsp90 and activates its ATPase ..
  12. pmc Asymmetric Hsp90 N domain SUMOylation recruits Aha1 and ATP-competitive inhibitors
    Mehdi Mollapour
    Department of Urology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA Cancer Research Institute, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, 9000 Rockville Pike, Bethesda, MD 20892, USA Electronic address
    Mol Cell 53:317-29. 2014
    ..and human (K191) Hsp90 facilitates both recruitment of the adenosine triphosphatase (ATPase)-activating cochaperone Aha1 and, unexpectedly, the binding of Hsp90 inhibitors, suggesting that these drugs associate preferentially with Hsp90 ..
  13. doi Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle
    Jing Li
    Center for Integrated Protein Science, Department Chemie, Technische Universitat Munchen, Munchen, Germany
    Nat Struct Mol Biol 20:326-31. 2013
    ..Here we analyzed the role of the activating co-chaperone Aha1 in the progression of the yeast Hsp90 chaperone cycle and identified a critical ternary Hsp90 complex containing ..
  14. pmc The co-chaperone Hch1 regulates Hsp90 function differently than its homologue Aha1 and confers sensitivity to yeast to the Hsp90 inhibitor NVP-AUY922
    Heather Armstrong
    Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada
    PLoS ONE 7:e49322. 2012
    ..Deletion of HCH1, but not AHA1, mitigates the temperature sensitive phenotype and high sensitivity to Hsp90 inhibitor drugs observed in ..
  15. doi Mixed Hsp90-cochaperone complexes are important for the progression of the reaction cycle
    Jing Li
    Center for Integrated Protein Science, Department Chemie, Technische Universitat Munchen, Munich, Germany
    Nat Struct Mol Biol 18:61-6. 2011
    ..This mechanism, which is strictly conserved between the yeast and human Hsp90 systems, presents an example of how, in a cyclic process, directionality of assembly and disassembly of protein complexes can be achieved...
  16. pmc Hsp90 cochaperone Aha1 is a negative regulator of the Saccharomyces MAL activator and acts early in the chaperone activation pathway
    Fulai Ran
    Biology Department, Queens College City University of New York, Flushing, New York 11367, USA
    J Biol Chem 285:13850-62. 2010
    b>Aha1 is a ubiquitous cochaperone of the Hsp90/Hsp70 chaperone machine. It binds the middle domain of Hsp90 and stimulates ATPase activity, suggesting a function late in the chaperone pathway...
  17. pmc Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery
    Philippe Meyer
    Chester Beatty Laboratories, Section of Structural Biology, The Institute of Cancer Research, London, UK
    EMBO J 23:511-9. 2004
    ..The co-chaperones Aha1 and Hch1 bind to Hsp90 and stimulate its ATPase activity...
  18. ncbi Structural and functional analysis of the middle segment of hsp90: implications for ATP hydrolysis and client protein and cochaperone interactions
    Philippe Meyer
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, SW3 6JB, London, United Kingdom
    Mol Cell 11:647-58. 2003
    ..structure, residues implicated in the ATPase-coupled conformational cycle and in interactions with client proteins and the activating cochaperone Aha1 have been identified, and their roles functionally characterized in vitro and in vivo.