Kcna2

Summary

Gene Symbol: Kcna2
Description: potassium channel, voltage gated shaker related subfamily A, member 2
Alias: BK2, NGK1, Potassium (K+) channel protein alpha 2, voltage dependent, RAK, RBK2, RCK5, potassium voltage gated channel shaker related subfamily member 2, potassium voltage-gated channel subfamily A member 2, potassium voltage-gated channel, shaker-related subfamily, member 2, voltage-gated potassium channel subunit Kv1.2
Species: rat

Top Publications

  1. ncbi Crystal structure of a mammalian voltage-dependent Shaker family K+ channel
    Stephen B Long
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:897-903. 2005
  2. ncbi Beta subunits promote K+ channel surface expression through effects early in biosynthesis
    G Shi
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794, USA
    Neuron 16:843-52. 1996
  3. ncbi Contributions of Kv1.2, Kv1.5 and Kv2.1 subunits to the native delayed rectifier K(+) current in rat mesenteric artery smooth muscle cells
    Yanjie Lu
    Department of Physiology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, Canada S7N 5E5
    Life Sci 71:1465-73. 2002
  4. pmc Cellular mechanisms and behavioral consequences of Kv1.2 regulation in the rat cerebellum
    Michael R Williams
    Neuroscience Graduate Program, Department of Pharmacology, University of Vermont, Burlington, Vermont 05405, USA
    J Neurosci 32:9228-37. 2012
  5. pmc Dynamics of Kv1 channel transport in axons
    Yuanzheng Gu
    Department of Neuroscience and Center for Molecular Neurobiology, The Ohio State University, Columbus, Ohio, USA
    PLoS ONE 5:e11931. 2010
  6. pmc Trafficking-dependent phosphorylation of Kv1.2 regulates voltage-gated potassium channel cell surface expression
    Jae Won Yang
    Section of Neurobiology, Physiology, and Behavior, College of Biological Sciences, School of Medicine, University of California, Davis, CA 95616, USA
    Proc Natl Acad Sci U S A 104:20055-60. 2007
  7. doi Voltage gated K+ channel expression in arteries of Wistar-Kyoto and spontaneously hypertensive rats
    Robert H Cox
    Department of Pharmacology, Lankenau Institute for Medical Research, Main Line Health System, Wynnewood, Pennsylvania, USA
    Am J Hypertens 21:213-8. 2008
  8. pmc Atomic constraints between the voltage sensor and the pore domain in a voltage-gated K+ channel of known structure
    Anthony Lewis
    Department of Pediatrics, University of Chicago, Chicago, IL 60637, USA
    J Gen Physiol 131:549-61. 2008
  9. doi Expression of Kv1.2 in microglia and its putative roles in modulating production of proinflammatory cytokines and reactive oxygen species
    Fan Li
    Faculty of Basic Medical Sciences, Kunming Medical College, Kunming, China
    J Neurochem 106:2093-105. 2008
  10. pmc Clustering and activity tuning of Kv1 channels in myelinated hippocampal axons
    Chen Gu
    Department of Neuroscience and Center for Molecular Neurobiology, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 286:25835-47. 2011

Research Grants

Scientific Experts

  • Robert H Cox
  • D L Minor
  • Chen Gu
  • Li Li Zhao
  • Vishwanath Jogini
  • Anthony Lewis
  • Michael R Williams
  • Xiaorui Chen
  • Yuanzheng Gu
  • Ahmed Al-Sabi
  • Morten Ø Jensen
  • Li Jun Bi
  • Zhi Qi
  • Gang Jin
  • Xian En Zhang
  • Xiao Tao
  • Fan Li
  • Benoit Roux
  • Aaron H Bubolz
  • Anthony D Morielli
  • Qiang Chai
  • Julio F Cordero-Morales
  • Jae Won Yang
  • Roderick MacKinnon
  • Tetsuhiro Fujita
  • Thomas F Finnegan
  • Elena Caminos
  • Stephen B Long
  • Paul D Dodson
  • David Hattan
  • Yanjie Lu
  • John T Green
  • Jason R Fuchs
  • Y A Kuryshev
  • Kresten Lindorff-Larsen
  • Fengyun Ni
  • Michael P Eastwood
  • J Oliver Dolly
  • Jon T Sack
  • Marie Le Berre
  • Sorcha Ni Dhochartaigh
  • Ron O Dror
  • Paul Maragakis
  • Oleg Shamotienko
  • David W Borhani
  • Jiafu Wang
  • Nagesh Muniyappa
  • Jianpeng Ma
  • Hamdy Shaban
  • David E Shaw
  • Qinghua Wang
  • Taijiao Jiang
  • Pei Liu
  • Aiping Wu
  • Shuqing Li
  • Jun Sun
  • Viswanathan Sivakumar
  • Eng Ang Ling
  • Jia Lu
  • Charanjit Kaur
  • Lydia Blachowicz
  • Chun Yun Wu
  • Muriel Laine
  • S Poliak
  • Qing Jia
  • Helene Vacher
  • Qiang Dong
  • Valeria Vásquez
  • Zhixiang Liu
  • David D Gutterman
  • Xiaoqun Xu
  • Eliana Clark
  • Yanping Liu
  • Weidong Zhang
  • D Marien Cortes
  • James S Trimmer
  • Eduardo Perozo
  • Brandon T Larsen
  • Lianbi Chen
  • Kang Sik Park
  • Qingping Wu
  • Jin Ren
  • Iku Utsunomiya
  • Shao Rui Chen
  • Keiko Hoshi
  • Hui Lin Pan
  • Sachie Sasaki
  • Yousuke Matsushita
  • Miwa Kawai
  • Tadashi Miyatake

Detail Information

Publications31

  1. ncbi Crystal structure of a mammalian voltage-dependent Shaker family K+ channel
    Stephen B Long
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:897-903. 2005
    ..Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit...
  2. ncbi Beta subunits promote K+ channel surface expression through effects early in biosynthesis
    G Shi
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794, USA
    Neuron 16:843-52. 1996
    ..These findings provide a molecular basis for recent genetic studies indicating that beta subunits are key determinants of neuronal excitability...
  3. ncbi Contributions of Kv1.2, Kv1.5 and Kv2.1 subunits to the native delayed rectifier K(+) current in rat mesenteric artery smooth muscle cells
    Yanjie Lu
    Department of Physiology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, Canada S7N 5E5
    Life Sci 71:1465-73. 2002
    ..05). A control antibody (anti-GIRK1) also had no effect on the native Kv currents. This study demonstrates that Kv1.2, Kv1.5, and Kv2.1 subunit genes all contribute to the formation of the native Kv channels in rat mesenteric artery SMCs...
  4. pmc Cellular mechanisms and behavioral consequences of Kv1.2 regulation in the rat cerebellum
    Michael R Williams
    Neuroscience Graduate Program, Department of Pharmacology, University of Vermont, Burlington, Vermont 05405, USA
    J Neurosci 32:9228-37. 2012
    ..2 endocytosis is supported by our finding that infusion into the cerebellar cortex of either the Kv1.2 inhibitor tityustoxin-Kα, or of the Kv1.2 regulator secretin, significantly enhances acquisition of eyeblink conditioning in rats...
  5. pmc Dynamics of Kv1 channel transport in axons
    Yuanzheng Gu
    Department of Neuroscience and Center for Molecular Neurobiology, The Ohio State University, Columbus, Ohio, USA
    PLoS ONE 5:e11931. 2010
    ..2 tetramers in axonal puncta. Taken together, our results suggest that proper amounts of Kv1 channels and their associated proteins are required for efficient transport of Kv1 channel proteins along axons...
  6. pmc Trafficking-dependent phosphorylation of Kv1.2 regulates voltage-gated potassium channel cell surface expression
    Jae Won Yang
    Section of Neurobiology, Physiology, and Behavior, College of Biological Sciences, School of Medicine, University of California, Davis, CA 95616, USA
    Proc Natl Acad Sci U S A 104:20055-60. 2007
    ..These data support Kv1.2 phosphorylation at these clustered C-terminal sites as playing an important role in regulating trafficking of Kv1.2-containing Kv channels...
  7. doi Voltage gated K+ channel expression in arteries of Wistar-Kyoto and spontaneously hypertensive rats
    Robert H Cox
    Department of Pharmacology, Lankenau Institute for Medical Research, Main Line Health System, Wynnewood, Pennsylvania, USA
    Am J Hypertens 21:213-8. 2008
    ..X channel alpha-subunits in arteries from Wistar-Kyoto rats (WKYs) and spontaneously hypertensive rats (SHRs). The purpose of this study was to test the hypothesis that these differences are also present at the protein level...
  8. pmc Atomic constraints between the voltage sensor and the pore domain in a voltage-gated K+ channel of known structure
    Anthony Lewis
    Department of Pediatrics, University of Chicago, Chicago, IL 60637, USA
    J Gen Physiol 131:549-61. 2008
    ..2 x-ray structure. A structural model is provided for this conformation. The results further highlight the dynamic and flexible nature of the voltage sensor...
  9. doi Expression of Kv1.2 in microglia and its putative roles in modulating production of proinflammatory cytokines and reactive oxygen species
    Fan Li
    Faculty of Basic Medical Sciences, Kunming Medical College, Kunming, China
    J Neurochem 106:2093-105. 2008
    ..We conclude that Kv1.2 in microglia modulates IL-1beta and TNF-alpha expression and ROS production probably by regulating the intracellular potassium concentration...
  10. pmc Clustering and activity tuning of Kv1 channels in myelinated hippocampal axons
    Chen Gu
    Department of Neuroscience and Center for Molecular Neurobiology, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 286:25835-47. 2011
    ..This effect was eliminated by the Tyr⁴⁵⁸ mutation or by cholesterol depletion. Taken together, our studies suggest that myelin regulates both trafficking and activity of Kv1 channels along hippocampal axons through TAG-1...
  11. pmc Functional analysis of Kv1.2 and paddle chimera Kv channels in planar lipid bilayers
    Xiao Tao
    Howard Hughes Medical Institute, Department of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    J Mol Biol 382:24-33. 2008
    ..2. We find, however, that in several respects, the Kv1.2 channel exhibits functional properties that are distinct from Kv1.2 channels reported in the literature...
  12. doi Length-dependent regulation of the Kv1.2 channel activation by its C-terminus
    Li Li Zhao
    State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, PR China
    Mol Membr Biol 26:186-93. 2009
    ..2 channel. These results imply that the dynamic interaction of the C-terminus with the S4-S5 linker from a neighboring subunit of the Kv1.2 channel provides a mechanism for its C-terminus to regulate the channel activation...
  13. pmc Arrangement of Kv1 alpha subunits dictates sensitivity to tetraethylammonium
    Ahmed Al-Sabi
    International Centre for Neurotherapeutics, Dublin City University, Dublin 9, Ireland
    J Gen Physiol 136:273-82. 2010
    ..These collective findings indicate that assembly of alpha subunits can be directed by this optimized concatenation, and that subunit arrangement in heteromeric Kv channels affects TEA affinity...
  14. doi Regulatory role of the extreme C-terminal end of the S6 inner helix in C-terminal-truncated Kv1.2 channel activation
    Li Li Zhao
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    Cell Biol Int 34:433-9. 2010
    ..Hence, our results indicate that the extreme C-terminal end of the S6 inner helix plays an important regulatory role in the activation of the C-terminal-truncated Kv1.2 channel...
  15. pmc Principles of conduction and hydrophobic gating in K+ channels
    Morten Ø Jensen
    DE Shaw Research, New York, NY 10036, USA
    Proc Natl Acad Sci U S A 107:5833-8. 2010
    ....
  16. pmc Structure of the full-length Shaker potassium channel Kv1.2 by normal-mode-based X-ray crystallographic refinement
    Xiaorui Chen
    Graduate Program of Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 107:11352-7. 2010
    ..2 between the internal and external solutions. This work also demonstrated the potential of the refinement method in bringing up large chunks of missing densities, thus beneficial to structural refinement of many difficult systems...
  17. ncbi Molecular basis of dysfunctional Kv channels in small coronary artery smooth muscle cells of streptozotocin-induced diabetic rats
    Qiang Chai
    Department of Physiology, Shandong Academy of Medical Sciences, Jinan 250062, Shandong, People s Republic of China
    Chin J Physiol 50:171-7. 2007
    ....
  18. ncbi Molecular driving forces determining potassium channel slow inactivation
    Julio F Cordero-Morales
    Department of Molecular Physiology and Biological Physics, University of Virginia, 1300 JPA, Charlottesville, Virginia 22908, USA
    Nat Struct Mol Biol 14:1062-9. 2007
    ..2 suggests that a similar mechanistic principle applies to other K+ channels. These observations provide a plausible physical framework for understanding C-type inactivation in K+ channels...
  19. pmc Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain
    W Stuhmer
    Max Planck Institut fur biophysikalische Chemie, Abteilung Membranbiophysik, Gottingen, FRG
    EMBO J 8:3235-44. 1989
    ....
  20. ncbi Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene family
    D McKinnon
    Department of Pharmacology, Washington University Medical School, St Louis, Missouri 63110
    J Biol Chem 264:8230-6. 1989
    ..This new potassium channel is called BK2 to distinguish it from the previously described potassium channel (BK1)...
  21. ncbi Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases
    E Kim
    Howard Hughes Medical Institute, Massachusetts General Hospital, Department of Neurobiology, Harvard Medical School, Boston 02114, USA
    Nature 378:85-8. 1995
    ..3) and syntrophin), suggest that PDZ-domain-containing polypeptides may be widely involved in the organization of proteins at sites of membrane specialization...
  22. ncbi Caspr2, a new member of the neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels
    S Poliak
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Neuron 24:1037-47. 1999
    ..This association involves the C-terminal sequence of Caspr2, which contains a putative PDZ binding site. These results suggest a role for Caspr family members in the local differentiation of the axon into distinct functional subdomains...
  23. ncbi The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel
    D L Minor
    Howard Hughes Medical Institute and Department of Physiology, University of California, San Francisco 94143, USA
    Cell 102:657-70. 2000
    ..Together, these data suggest that structural changes involving the buried polar T1 surfaces play a key role in the conformational changes leading to channel opening...
  24. ncbi KChAP/Kvbeta1.2 interactions and their effects on cardiac Kv channel expression
    Y A Kuryshev
    The Rammelkamp Center for Education and Research, MetroHealth Campus, Cleveland, Ohio 44109, USA
    Am J Physiol Cell Physiol 281:C290-9. 2001
    ..2 may alter both sustained and transient cardiac Kv currents. The interaction of these two different classes of modulatory proteins may constitute a novel mechanism for regulating cardiac K+ currents...
  25. ncbi Tyrosine phosphorylation of Kv1.2 modulates its interaction with the actin-binding protein cortactin
    David Hattan
    Department of Pharmacology, University of Vermont College of Medicine, Burlington, Vermont 04505, USA
    J Biol Chem 277:38596-606. 2002
    ..These results demonstrate a dynamic, phosphorylation-dependent interaction between Kv1.2 and the actin cytoskeleton-binding protein cortactin and suggest a role for that interaction in the regulation of Kv1.2 ionic current...
  26. pmc Presynaptic rat Kv1.2 channels suppress synaptic terminal hyperexcitability following action potential invasion
    Paul D Dodson
    Department of Cell Physiology and Pharmacology, University of Leicester, PO Box 138, Leicester LE1 9HN, UK
    J Physiol 550:27-33. 2003
    ..We conclude that Kv1.2 channels have a general presynaptic function in suppressing terminal hyperexcitability during the depolarising after-potential...
  27. ncbi Developmental regulation and adult maintenance of potassium channel proteins (Kv 1.1 and Kv 1.2) in the cochlear nucleus of the rat
    Elena Caminos
    Facultad de Medicina and Centro Regional de Investigaciones Biomédicas CRIB, Universidad de Castilla La Mancha, 02006 Albacete, Spain
    Brain Res 1056:118-31. 2005
    ..Thus, normal excitatory synaptic input in adult animals is not a requirement to regulate the expression and cellular and subcellular distribution of these potassium channel proteins...
  28. ncbi Mu opioid receptor activation inhibits GABAergic inputs to basolateral amygdala neurons through Kv1.1/1.2 channels
    Thomas F Finnegan
    Department of Anesthesiology, Pennsylvania State University College of Medicine, The Milton S Hershey Medical Center, Hershey Pennsylvania, USA
    J Neurophysiol 95:2032-41. 2006
    ..This study provides new information that activation of presynaptic mu opioid receptors primarily attenuates GABAergic synaptic inputs to CeA-projecting neurons in the BLA through a signaling mechanism involving Kv1.1 and Kv1.2 channels...
  29. ncbi Glycosylation and cell surface expression of Kv1.2 potassium channel are regulated by determinants in the pore region
    Tetsuhiro Fujita
    Department of Pharmacotherapeutics, Showa Pharmaceutical University, Tokyo, Machida, Japan
    Neurochem Res 31:589-96. 2006
    ..2 and cells coexpressing beta2and S360A. These results suggest that amino acids in the pore region help regulate ion permeability or cellular trafficking by affecting glycosylation of Kv1.2...
  30. ncbi Ebselen reduces nitration and restores voltage-gated potassium channel function in small coronary arteries of diabetic rats
    Aaron H Bubolz
    Department of Medicine, The Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Am J Physiol Heart Circ Physiol 293:H2231-7. 2007
    ..Eb may be beneficial for the therapeutic treatment of vascular complications in diabetes mellitus...
  31. pmc Cloning and expression of a rat cardiac delayed rectifier potassium channel
    M Paulmichl
    Department of Pharmacology, Mayo Foundation, Rochester, MN 55905
    Proc Natl Acad Sci U S A 88:7892-5. 1991
    ..RAK differs from the cloned rat brain K current, BK2 [McKinnon, D. (1989) J. Biol. Chem. 264, 8230-8236], by one amino acid at residue 411...

Research Grants1

  1. HYPERTENSION: CONTRIBUTION OF ARTERIAL WALL CHANGES
    Robert Cox; Fiscal Year: 2007
    ..These studies will define a novel therapeutic target for antihypertensive drug development based upon the mechanism(s) of Ca2+-Kv channel modulation. ..