Genomes and Genes
Gene Symbol: Kcna2
Description: potassium voltage-gated channel subfamily A member 2
Alias: BK2, NGK1, potassium voltage-gated channel subfamily A member 2, Potassium (K+) channel protein alpha 2, voltage dependent, Potassium voltage gated channel shaker related subfamily member 2, RAK, RBK2, RCK5, potassium channel, voltage gated shaker related subfamily A, member 2, potassium voltage-gated channel, shaker-related subfamily, member 2, voltage-gated potassium channel subunit Kv1.2
- Crystal structure of a mammalian voltage-dependent Shaker family K+ channelStephen B Long
Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Science 309:897-903. 2005..Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit...
- Functional analysis of Kv1.2 and paddle chimera Kv channels in planar lipid bilayersXiao Tao
Howard Hughes Medical Institute, Department of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
J Mol Biol 382:24-33. 2008..2. We find, however, that in several respects, the Kv1.2 channel exhibits functional properties that are distinct from Kv1.2 channels reported in the literature...
- Structure of the full-length Shaker potassium channel Kv1.2 by normal-mode-based X-ray crystallographic refinementXiaorui Chen
Graduate Program of Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 107:11352-7. 2010..2 between the internal and external solutions. This work also demonstrated the potential of the refinement method in bringing up large chunks of missing densities, thus beneficial to structural refinement of many difficult systems...
- The role of Kif5B in axonal localization of Kv1 K(+) channelsJacqueline Rivera
University of Southern California, Department of Biological Sciences, Division of Molecular and Computational Biology, Program in Neuroscience, 1050 Childs Way MCB 204, Los Angeles, CA 90089 2910, USA
Eur J Neurosci 25:136-46. 2007..Together these data suggest a model where Kif5B interacts with Kv1 channels either directly or indirectly via the T1 domain, causing the channels to be transported to axons...
- A long noncoding RNA contributes to neuropathic pain by silencing Kcna2 in primary afferent neuronsXiuli Zhao
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Nat Neurosci 16:1024-31. 2013..Here we identify a conserved lncRNA, named Kcna2 antisense RNA, for a voltage-dependent potassium channel mRNA, Kcna2, in first-order sensory neurons of rat dorsal ..
- Beta subunits promote K+ channel surface expression through effects early in biosynthesisG Shi
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794, USA
Neuron 16:843-52. 1996..These findings provide a molecular basis for recent genetic studies indicating that beta subunits are key determinants of neuronal excitability...
- Exercise training reverses alterations in Kv and BKCa channel molecular expression in thoracic aorta smooth muscle cells from spontaneously hypertensive ratsZhencheng Li
Department of Exercise Physiology, Beijing Sport University, Beijing, China
J Vasc Res 51:447-57. 2014..These data suggest that exercise training reverses the pathological expression of the Kv1.2, Kv1.5, and BKCa channels in aortic myocytes from SHRs. This is one of the favorable effects of exercise training on large conduit arteries...
- Ion Concentration- and Voltage-Dependent Push and Pull Mechanisms of Potassium Channel Ion ConductionKota Kasahara
Graduate School of Information Sciences, Tohoku University, 6 3 09, Aramaki Aza Aoba, Aoba ku, Sendai, Miyagi 980 8597, Japan
PLoS ONE 11:e0150716. 2016....
- The molecular basis for the actions of KVbeta1.2 on the opening and closing of the KV1.2 delayed rectifier channelChristian J Peters
Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
Channels (Austin) 3:314-22. 2009..Together with data from previous studies, our findings provide a complete and coherent picture of the functional and structural interactions between K(V)beta1.2 and K(V)1.2...
- ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axonsYasuhiro Ogawa
Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
J Neurosci 30:1038-48. 2010..2 and Caspr2 clustering is normal in ADAM22-null mice. Thus, ADAM22 is an axonal component of the Kv1 K(+) channel complex that recruits MAGUKs to juxtaparanodes...
- Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteinsKatrine Hommelhoff Jensen
Department of Computer Science, University of Copenhagen, Universitetsparken 5, DK 2100 Copenhagen, Denmark
J Struct Biol 194:49-60. 2016..Precise removal of the strong membrane signals allows better alignment and classification of images of small membrane-protein particles, and allows higher-resolution 3D reconstruction. ..
- Kv1.2 mediates heterosynaptic modulation of direct cortical synaptic inputs in CA3 pyramidal cellsJung Ho Hyun
Cell Physiology Laboratory, Department of Physiology and bioMembrane Plasticity Research Centre, Seoul National University College of Medicine and Neuroscience Research Institute, Seoul National University Medical Research Centre, 103 Daehak Ro, Jongno gu, Seoul, 110 799, Republic of Korea
J Physiol 593:3617-43. 2015..2. Our results may provide new insights into the long-standing question of how mossy fibre inputs constrain the CA3 network to sparsely represent direct cortical inputs...
- Structure of a pore-blocking toxin in complex with a eukaryotic voltage-dependent K(+) channelAnirban Banerjee
Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, New York, United States Howard Hughes Medical Institute, Rockefeller University, New York, United States
elife 2:e00594. 2013..Together, these results show that CTX binds to a K(+) channel in a lock and key manner and interacts directly with conducting ions inside the selectivity filter. DOI:http://dx.doi.org/10.7554/eLife.00594.001...
- Pharmacological characteristics of Kv1.1- and Kv1.2-containing channels are influenced by the stoichiometry and positioning of their α subunitsAhmed Al-Sabi
International Centre for Neurotherapeutics, Dublin City University, Glasnevin, Dublin 9, Ireland
Biochem J 454:101-8. 2013..This is important in designing drugs with abilities to inhibit particular oligomeric Kv1 subtypes, with the goal of elevating neuronal excitability and improving neurotransmission in certain diseases. ..
- Two heteromeric Kv1 potassium channels differentially regulate action potential firingPaul D Dodson
Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, LE1 9HN, United Kingdom
J Neurosci 22:6953-61. 2002..Our results show that heteromeric channels containing Kv1.2 subunits govern AP firing and suggest that their localization at the initial segment of MNTB axons can explain their dominance of AP firing behavior...
- Contributions of Kv1.2, Kv1.5 and Kv2.1 subunits to the native delayed rectifier K(+) current in rat mesenteric artery smooth muscle cellsYanjie Lu
Department of Physiology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, Canada S7N 5E5
Life Sci 71:1465-73. 2002..05). A control antibody (anti-GIRK1) also had no effect on the native Kv currents. This study demonstrates that Kv1.2, Kv1.5, and Kv2.1 subunit genes all contribute to the formation of the native Kv channels in rat mesenteric artery SMCs...
- Impaired neuropathic pain and preserved acute pain in rats overexpressing voltage-gated potassium channel subunit Kv1.2 in primary afferent neuronsLongchang Fan
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Mol Pain 10:8. 2014..2 antisense RNA, in addition to the increase in Kv1.2 protein expression, in the injured DRG. Our findings suggest that Kv1.2 may be a novel potential target for preventing and/or treating neuropathic pain. ..
- Caution is required in interpretation of mutations in the voltage sensing domain of voltage gated channels as evidence for gating mechanismsAlisher M Kariev
Department of Chemistry, City College of New York, 160 Convent Avenue, New York, NY 10031, USA
Int J Mol Sci 16:1627-43. 2015..The computations strongly suggest that the interpretation of cysteine substitution reaction experiments be re-examined in the light of these considerations...
- Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexesK J Rhodes
Central Nervous System Disorders, Wyeth Ayerst Research, Princeton, New Jersey 08543, USA
J Neurosci 17:8246-58. 1997....
- Cellular mechanisms and behavioral consequences of Kv1.2 regulation in the rat cerebellumMichael R Williams
Neuroscience Graduate Program, Department of Pharmacology, University of Vermont, Burlington, Vermont 05405, USA
J Neurosci 32:9228-37. 2012..2 endocytosis is supported by our finding that infusion into the cerebellar cortex of either the Kv1.2 inhibitor tityustoxin-Kα, or of the Kv1.2 regulator secretin, significantly enhances acquisition of eyeblink conditioning in rats...
- Voltage gated K+ channel expression in arteries of Wistar-Kyoto and spontaneously hypertensive ratsRobert H Cox
Department of Pharmacology, Lankenau Institute for Medical Research, Main Line Health System, Wynnewood, Pennsylvania, USA
Am J Hypertens 21:213-8. 2008..X channel alpha-subunits in arteries from Wistar-Kyoto rats (WKYs) and spontaneously hypertensive rats (SHRs). The purpose of this study was to test the hypothesis that these differences are also present at the protein level...
- Clustering and activity tuning of Kv1 channels in myelinated hippocampal axonsChen Gu
Department of Neuroscience and Center for Molecular Neurobiology, Ohio State University, Columbus, Ohio 43210, USA
J Biol Chem 286:25835-47. 2011..This effect was eliminated by the Tyr⁴⁵⁸ mutation or by cholesterol depletion. Taken together, our studies suggest that myelin regulates both trafficking and activity of Kv1 channels along hippocampal axons through TAG-1...
- Arrangement of Kv1 alpha subunits dictates sensitivity to tetraethylammoniumAhmed Al-Sabi
International Centre for Neurotherapeutics, Dublin City University, Dublin 9, Ireland
J Gen Physiol 136:273-82. 2010..These collective findings indicate that assembly of alpha subunits can be directed by this optimized concatenation, and that subunit arrangement in heteromeric Kv channels affects TEA affinity...
- Dynamics of Kv1 channel transport in axonsYuanzheng Gu
Department of Neuroscience and Center for Molecular Neurobiology, The Ohio State University, Columbus, Ohio, USA
PLoS ONE 5:e11931. 2010..2 tetramers in axonal puncta. Taken together, our results suggest that proper amounts of Kv1 channels and their associated proteins are required for efficient transport of Kv1 channel proteins along axons...
- Principles of conduction and hydrophobic gating in K+ channelsMorten Ø Jensen
DE Shaw Research, New York, NY 10036, USA
Proc Natl Acad Sci U S A 107:5833-8. 2010....
- Regulatory role of the extreme C-terminal end of the S6 inner helix in C-terminal-truncated Kv1.2 channel activationLi Li Zhao
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
Cell Biol Int 34:433-9. 2010..Hence, our results indicate that the extreme C-terminal end of the S6 inner helix plays an important regulatory role in the activation of the C-terminal-truncated Kv1.2 channel...
- Mu opioid receptor activation inhibits GABAergic inputs to basolateral amygdala neurons through Kv1.1/1.2 channelsThomas F Finnegan
Department of Anesthesiology, Pennsylvania State University College of Medicine, The Milton S Hershey Medical Center, Hershey Pennsylvania, USA
J Neurophysiol 95:2032-41. 2006..This study provides new information that activation of presynaptic mu opioid receptors primarily attenuates GABAergic synaptic inputs to CeA-projecting neurons in the BLA through a signaling mechanism involving Kv1.1 and Kv1.2 channels...
- Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brainW Stuhmer
Max Planck Institut fur biophysikalische Chemie, Abteilung Membranbiophysik, Gottingen, FRG
EMBO J 8:3235-44. 1989....
- Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene familyD McKinnon
Department of Pharmacology, Washington University Medical School, St Louis, Missouri 63110
J Biol Chem 264:8230-6. 1989..This new potassium channel is called BK2 to distinguish it from the previously described potassium channel (BK1)...
- Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinasesE Kim
Howard Hughes Medical Institute, Massachusetts General Hospital, Department of Neurobiology, Harvard Medical School, Boston 02114, USA
Nature 378:85-8. 1995..3) and syntrophin), suggest that PDZ-domain-containing polypeptides may be widely involved in the organization of proteins at sites of membrane specialization...
- Caspr2, a new member of the neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channelsS Poliak
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Neuron 24:1037-47. 1999..This association involves the C-terminal sequence of Caspr2, which contains a putative PDZ binding site. These results suggest a role for Caspr family members in the local differentiation of the axon into distinct functional subdomains...
- The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channelD L Minor
Howard Hughes Medical Institute and Department of Physiology, University of California, San Francisco 94143, USA
Cell 102:657-70. 2000..Together, these data suggest that structural changes involving the buried polar T1 surfaces play a key role in the conformational changes leading to channel opening...
- KChAP/Kvbeta1.2 interactions and their effects on cardiac Kv channel expressionY A Kuryshev
The Rammelkamp Center for Education and Research, MetroHealth Campus, Cleveland, Ohio 44109, USA
Am J Physiol Cell Physiol 281:C290-9. 2001..2 may alter both sustained and transient cardiac Kv currents. The interaction of these two different classes of modulatory proteins may constitute a novel mechanism for regulating cardiac K+ currents...
- Tyrosine phosphorylation of Kv1.2 modulates its interaction with the actin-binding protein cortactinDavid Hattan
Department of Pharmacology, University of Vermont College of Medicine, Burlington, Vermont 04505, USA
J Biol Chem 277:38596-606. 2002..These results demonstrate a dynamic, phosphorylation-dependent interaction between Kv1.2 and the actin cytoskeleton-binding protein cortactin and suggest a role for that interaction in the regulation of Kv1.2 ionic current...
- Presynaptic rat Kv1.2 channels suppress synaptic terminal hyperexcitability following action potential invasionPaul D Dodson
Department of Cell Physiology and Pharmacology, University of Leicester, PO Box 138, Leicester LE1 9HN, UK
J Physiol 550:27-33. 2003..We conclude that Kv1.2 channels have a general presynaptic function in suppressing terminal hyperexcitability during the depolarising after-potential...
- Developmental regulation and adult maintenance of potassium channel proteins (Kv 1.1 and Kv 1.2) in the cochlear nucleus of the ratElena Caminos
Facultad de Medicina and Centro Regional de Investigaciones Biomédicas CRIB, Universidad de Castilla La Mancha, 02006 Albacete, Spain
Brain Res 1056:118-31. 2005..Thus, normal excitatory synaptic input in adult animals is not a requirement to regulate the expression and cellular and subcellular distribution of these potassium channel proteins...
- Length-dependent regulation of the Kv1.2 channel activation by its C-terminusLi Li Zhao
State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, PR China
Mol Membr Biol 26:186-93. 2009..2 channel. These results imply that the dynamic interaction of the C-terminus with the S4-S5 linker from a neighboring subunit of the Kv1.2 channel provides a mechanism for its C-terminus to regulate the channel activation...
- Glycosylation and cell surface expression of Kv1.2 potassium channel are regulated by determinants in the pore regionTetsuhiro Fujita
Department of Pharmacotherapeutics, Showa Pharmaceutical University, Tokyo, Machida, Japan
Neurochem Res 31:589-96. 2006..2 and cells coexpressing beta2and S360A. These results suggest that amino acids in the pore region help regulate ion permeability or cellular trafficking by affecting glycosylation of Kv1.2...
- Ebselen reduces nitration and restores voltage-gated potassium channel function in small coronary arteries of diabetic ratsAaron H Bubolz
Department of Medicine, The Medical College of Wisconsin, Milwaukee, Wisconsin, USA
Am J Physiol Heart Circ Physiol 293:H2231-7. 2007..Eb may be beneficial for the therapeutic treatment of vascular complications in diabetes mellitus...
- Molecular driving forces determining potassium channel slow inactivationJulio F Cordero-Morales
Department of Molecular Physiology and Biological Physics, University of Virginia, 1300 JPA, Charlottesville, Virginia 22908, USA
Nat Struct Mol Biol 14:1062-9. 2007..2 suggests that a similar mechanistic principle applies to other K+ channels. These observations provide a plausible physical framework for understanding C-type inactivation in K+ channels...
- Molecular basis of dysfunctional Kv channels in small coronary artery smooth muscle cells of streptozotocin-induced diabetic ratsQiang Chai
Department of Physiology, Shandong Academy of Medical Sciences, Jinan 250062, Shandong, People s Republic of China
Chin J Physiol 50:171-7. 2007....
- Trafficking-dependent phosphorylation of Kv1.2 regulates voltage-gated potassium channel cell surface expressionJae Won Yang
Section of Neurobiology, Physiology, and Behavior, College of Biological Sciences, School of Medicine, University of California, Davis, CA 95616, USA
Proc Natl Acad Sci U S A 104:20055-60. 2007..These data support Kv1.2 phosphorylation at these clustered C-terminal sites as playing an important role in regulating trafficking of Kv1.2-containing Kv channels...
- Cloning and expression of a rat cardiac delayed rectifier potassium channelM Paulmichl
Department of Pharmacology, Mayo Foundation, Rochester, MN 55905
Proc Natl Acad Sci U S A 88:7892-5. 1991..RAK differs from the cloned rat brain K current, BK2 [McKinnon, D. (1989) J. Biol. Chem. 264, 8230-8236], by one amino acid at residue 411...
- Atomic constraints between the voltage sensor and the pore domain in a voltage-gated K+ channel of known structureAnthony Lewis
Department of Pediatrics, University of Chicago, Chicago, IL 60637, USA
J Gen Physiol 131:549-61. 2008..2 x-ray structure. A structural model is provided for this conformation. The results further highlight the dynamic and flexible nature of the voltage sensor...
- Expression of Kv1.2 in microglia and its putative roles in modulating production of proinflammatory cytokines and reactive oxygen speciesFan Li
Faculty of Basic Medical Sciences, Kunming Medical College, Kunming, China
J Neurochem 106:2093-105. 2008..We conclude that Kv1.2 in microglia modulates IL-1beta and TNF-alpha expression and ROS production probably by regulating the intracellular potassium concentration...
- HYPERTENSION: CONTRIBUTION OF ARTERIAL WALL CHANGESRobert Cox; Fiscal Year: 2007..These studies will define a novel therapeutic target for antihypertensive drug development based upon the mechanism(s) of Ca2+-Kv channel modulation. ..