eukaryotic elongation factor 2 kinase

Summary

Gene Symbol: eukaryotic elongation factor 2 kinase
Description: eukaryotic elongation factor-2 kinase
Alias: SMEF2K, eukaryotic elongation factor 2 kinase, calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, elongation factor 2 kinase, eukaroytic elongation factor 2 kinase
Species: rat

Top Publications

  1. ncbi Levels of mTOR and its downstream targets 4E-BP1, eEF2, and eEF2 kinase in relationships with tau in Alzheimer's disease brain
    Xu Li
    Division of Experimental Geriatrics, Department of Neurotec, Karolinska Institutet, Huddinge, Sweden
    FEBS J 272:4211-20. 2005
  2. pmc Oxygen and glucose deprivation induces widespread alterations in mRNA translation within 20 minutes
    Dmitry E Andreev
    School of Biochemistry and Cell Biology, Western Gateway Building, University College Cork, Cork, Ireland
    Genome Biol 16:90. 2015
  3. pmc Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation
    Tatsuya Usui
    Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan
    Am J Physiol Heart Circ Physiol 305:H756-68. 2013
  4. doi Synaptic activity controls dendritic spine morphology by modulating eEF2-dependent BDNF synthesis
    Chiara Verpelli
    Consiglio Nazionale delle Ricerche Neuroscience Institute, Milan, Italy
    J Neurosci 30:5830-42. 2010
  5. pmc Brain-derived neurotrophic factor enhances the basal rate of protein synthesis by increasing active eukaryotic elongation factor 2 levels and promoting translation elongation in cortical neurons
    Nobuyuki Takei
    Department of Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata 951 8585, Japan
    J Biol Chem 284:26340-8. 2009
  6. ncbi Characterization of the role of microtubule-associated protein 1B in metabotropic glutamate receptor-mediated endocytosis of AMPA receptors in hippocampus
    Genoveva Davidkova
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 27:13273-8. 2007
  7. pmc AMP-activated protein kinase protects cardiomyocytes against hypoxic injury through attenuation of endoplasmic reticulum stress
    Kazuo Terai
    Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita City, Osaka 565 0871, Japan
    Mol Cell Biol 25:9554-75. 2005
  8. ncbi Prolactin regulation of the calmodulin-dependent protein kinase III elongation factor-2 system in the rat corpus luteum
    C T Albarracin
    Department of Physiology and Biophysics, University of Illinois, Chicago 60612
    J Biol Chem 269:7772-6. 1994
  9. ncbi Myocardial ischemia and increased heart work modulate the phosphorylation state of eukaryotic elongation factor-2
    Sandrine Horman
    Hormone and Metabolic Research Unit, Christian de Duve Institute of Cellular Pathology, University of Louvain Medical School, Avenue Hippocrate 75, ICP UCL 7529, B 1200 Brussels, Belgium
    J Biol Chem 278:41970-6. 2003
  10. ncbi Localization of translational components at the ultramicroscopic level at postsynaptic sites of the rat brain
    Chie Asaki
    Department of Neuroplasticity, Research Center on Aging and Adaptation, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Brain Res 972:168-76. 2003

Research Grants

  1. THE ROLE OF EF-2 KINASE IN DRUG RESISTANCE
    ALEXEY RYAZANOV; Fiscal Year: 2003
  2. STRUCTURE/FUNCTION OF EFF-2 KINASE
    ALEXEY RYAZANOV; Fiscal Year: 2003
  3. THE ROLE OF PROTEIN TURNOVER IN AGING
    ALEXEY RYAZANOV; Fiscal Year: 2005

Scientific Experts

  • Nobuyuki Takei
  • T A Diggle
  • Genoveva Davidkova
  • Alexey G Ryazanov
  • Adam J Rose
  • Dmitry E Andreev
  • Sandrine Horman
  • Tatsuya Usui
  • Shari L Wiseman
  • Chiara Verpelli
  • Xu Li
  • Kazuo Terai
  • Gareth J Browne
  • Chie Asaki
  • Didier Vertommen
  • Pavel V Baranov
  • Patrick B F O'Connor
  • Alexander V Zhdanov
  • Ruslan I Dmitriev
  • Ivan N Shatsky
  • Louis Hue
  • Dmitri B Papkovsky
  • Muneyoshi Okada
  • Clive Palfrey
  • Hideyuki Yamawaki
  • Angus C Nairn
  • Yoshio Shimizu
  • Yukio Hara
  • Kun Huang
  • Giovanni Piccoli
  • Elena Battaglioli
  • Dario Brambilla
  • Monica Di Luca
  • Fabrizio Gardoni
  • Cristina Zibetti
  • Carlo Sala
  • Alice Zanchi
  • Shoko Sugiyama
  • Bengt Winblad
  • Mitsuru Masaki
  • Hisao Hirota
  • Hilkka Soininen
  • Jin Jing Pei
  • Irina Alafuzoff
  • Tadashi Kuroda
  • Masatsugu Hori
  • Ichiro Kawase
  • Yoshimune Hiramoto
  • Christopher G Proud
  • Stephen G Finn
  • Kiyokazu Kametani
  • Ayami Nakazawa
  • Mark H Rider
  • Nobuteru Usuda
  • Christophe Beauloye
  • Jean Louis Vanoverschelde
  • Tatsuo Suzuki
  • Mark Rider
  • N T Redpath
  • Alain Lavoinne
  • Luc Bertrand
  • Ulrike Krause
  • Gareth Browne
  • Christopher Proud
  • Jigna Patel
  • C T Albarracin
  • N T Price
  • C G Proud
  • H C Palfrey
  • M C Rao
  • W R Duan
  • G Gibori

Detail Information

Publications17

  1. ncbi Levels of mTOR and its downstream targets 4E-BP1, eEF2, and eEF2 kinase in relationships with tau in Alzheimer's disease brain
    Xu Li
    Division of Experimental Geriatrics, Department of Neurotec, Karolinska Institutet, Huddinge, Sweden
    FEBS J 272:4211-20. 2005
    ....
  2. pmc Oxygen and glucose deprivation induces widespread alterations in mRNA translation within 20 minutes
    Dmitry E Andreev
    School of Biochemistry and Cell Biology, Western Gateway Building, University College Cork, Cork, Ireland
    Genome Biol 16:90. 2015
    ..Oxygen and glucose metabolism play pivotal roles in many (patho)physiological conditions. In particular, oxygen and glucose deprivation (OGD) during ischemia and stroke results in extensive tissue injury and cell death...
  3. pmc Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation
    Tatsuya Usui
    Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan
    Am J Physiol Heart Circ Physiol 305:H756-68. 2013
    b>Eukaryotic elongation factor 2 kinase (eEF2K) is a Ca2+/calmodulin-dependent protein kinase. We recently demonstrated that eEF2K protein increases in mesenteric artery from spontaneously hypertensive rats (SHR)...
  4. doi Synaptic activity controls dendritic spine morphology by modulating eEF2-dependent BDNF synthesis
    Chiara Verpelli
    Consiglio Nazionale delle Ricerche Neuroscience Institute, Milan, Italy
    J Neurosci 30:5830-42. 2010
    ..We propose that the eEF2K/eEF2 pathway is a key biochemical sensor that couple neuronal activity to spine plasticity, by controlling the dendritic translation of BDNF...
  5. pmc Brain-derived neurotrophic factor enhances the basal rate of protein synthesis by increasing active eukaryotic elongation factor 2 levels and promoting translation elongation in cortical neurons
    Nobuyuki Takei
    Department of Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata 951 8585, Japan
    J Biol Chem 284:26340-8. 2009
    ..Taken together, our results demonstrate that the increased level of active eEF2 induced by chronic BDNF stimulation enhances translational elongation processes and increases the total rate of protein synthesis in neurons...
  6. ncbi Characterization of the role of microtubule-associated protein 1B in metabotropic glutamate receptor-mediated endocytosis of AMPA receptors in hippocampus
    Genoveva Davidkova
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 27:13273-8. 2007
    ..Although interfering with protein synthesis using short interfering RNA (siRNA) to eEF2 kinase (eukaryotic elongation factor 2 kinase) blocked the dendritic MAP1B increase by both pathways, it selectively blocked the DHPG- and not ..
  7. pmc AMP-activated protein kinase protects cardiomyocytes against hypoxic injury through attenuation of endoplasmic reticulum stress
    Kazuo Terai
    Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita City, Osaka 565 0871, Japan
    Mol Cell Biol 25:9554-75. 2005
    ....
  8. ncbi Prolactin regulation of the calmodulin-dependent protein kinase III elongation factor-2 system in the rat corpus luteum
    C T Albarracin
    Department of Physiology and Biophysics, University of Illinois, Chicago 60612
    J Biol Chem 269:7772-6. 1994
    ..These data provide strong evidence for a role of the EF-2/CaM kinase III system in PRL action in the corpus luteum...
  9. ncbi Myocardial ischemia and increased heart work modulate the phosphorylation state of eukaryotic elongation factor-2
    Sandrine Horman
    Hormone and Metabolic Research Unit, Christian de Duve Institute of Cellular Pathology, University of Louvain Medical School, Avenue Hippocrate 75, ICP UCL 7529, B 1200 Brussels, Belgium
    J Biol Chem 278:41970-6. 2003
    ..eEF2K activity was unchanged by increasing the workload, suggesting that the decrease in eEF2 phosphorylation could result from the activation of an eEF2 phosphatase...
  10. ncbi Localization of translational components at the ultramicroscopic level at postsynaptic sites of the rat brain
    Chie Asaki
    Department of Neuroplasticity, Research Center on Aging and Adaptation, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Brain Res 972:168-76. 2003
    ..The results also suggest the presence of ER-associated type of translational machinery at the postsynaptic sites...
  11. ncbi Activation of AMP-activated protein kinase leads to the phosphorylation of elongation factor 2 and an inhibition of protein synthesis
    Sandrine Horman
    Hormone and Metabolic Research Unit, Christian de Duve International Institute of Cellular Pathology and Université Catholique de Louvain, Avenue Hippocrate, 75, B 1200, Brussels, Belgium
    Curr Biol 12:1419-23. 2002
    ..Therefore, the activation of eEF2 kinase by AMPK, resulting in the phosphorylation and inactivation of eEF2, provides a novel mechanism for the inhibition of protein synthesis...
  12. pmc Regulation of protein-synthesis elongation-factor-2 kinase by cAMP in adipocytes
    T A Diggle
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, U K
    Biochem J 336:525-9. 1998
    ..These results demonstrate a novel mechanism for the control of protein synthesis by hormones which act by increasing cytoplasmic cAMP...
  13. pmc Proteasomal degradation of eukaryotic elongation factor-2 kinase (EF2K) is regulated by cAMP-PKA signaling and the SCFβTRCP ubiquitin E3 ligase
    Shari L Wiseman
    Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut 06508, USA
    J Biol Chem 288:17803-11. 2013
    ..We propose that the proteasomal degradation of EF2K provides a mechanistic link between activity-dependent protein synthesis and degradation...
  14. ncbi Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398
    Gareth J Browne
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 279:12220-31. 2004
    ..Thus, AMPK and eEF2 kinase may provide a key link between cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy...
  15. pmc A Ca(2+)-calmodulin-eEF2K-eEF2 signalling cascade, but not AMPK, contributes to the suppression of skeletal muscle protein synthesis during contractions
    Adam J Rose
    Copenhagen Muscle Research Centre and Molecular Physiology Group, Department of Exercise and Sport Sciences, University of Copenhagen, Denmark
    J Physiol 587:1547-63. 2009
    ..In summary, in fast-twitch skeletal muscle, the inhibition of eEF2 activity by phosphorylation downstream of Ca(2+)-CaM-eEF2K signalling partially contributes to the suppression of protein synthesis during exercise/contractions...
  16. pmc Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase
    A G Ryazanov
    Department of Pharmacology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 94:4884-9. 1997
    ..This suggests that eEF-2 kinase and MHCK A are members of a new class of protein kinases with a novel catalytic domain structure...
  17. ncbi Cloning and expression of cDNA encoding protein synthesis elongation factor-2 kinase
    N T Redpath
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 271:17547-54. 1996
    ..Northern blot analysis demonstrated that eEF-2K mRNA is expressed in a number of different tissues and that it may exist in multiple forms...

Research Grants3

  1. THE ROLE OF EF-2 KINASE IN DRUG RESISTANCE
    ALEXEY RYAZANOV; Fiscal Year: 2003
    ..Experiments will be performed to design and develop specific eEF-2 kinase inhibitors which can serve as pro- apoptotic agents. ..
  2. STRUCTURE/FUNCTION OF EFF-2 KINASE
    ALEXEY RYAZANOV; Fiscal Year: 2003
    ..This work will reveal the structural organization and function of a new class of protein kinases, which will provide new and important information about the mechanism of protein phosphorylation. ..
  3. THE ROLE OF PROTEIN TURNOVER IN AGING
    ALEXEY RYAZANOV; Fiscal Year: 2005
    ..The experiments described in this grant application are designed to provide a foundation for the development of novel therapeutic reagents that can mimic caloric restriction, circumventing some of the deleterious consequences of aging. ..