PLA2G1B

Summary

Gene Symbol: PLA2G1B
Description: phospholipase A2 group IB
Alias: PLA2, sPLA(2)-IB, phospholipase A2, major isoenzyme, group IB phospholipase A2, group IB secretory phospholipase A(2), phosphatidylcholine 2-acylhydrolase 1B, phospholipase A2, group IB (pancreas)
Species: pig

Top Publications

  1. pmc Solution structure of porcine pancreatic phospholipase A2
    B van den Berg
    Center for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands
    EMBO J 14:4123-31. 1995
  2. doi Thermodynamic reciprocity of the inhibitor binding to the active site and the interface binding region of IB phospholipase A2
    Otto G Berg
    Department of Molecular Evolution, Uppsala University Evolutionary Biology Center, Uppsala, Sweden
    Biochemistry 48:3209-18. 2009
  3. ncbi Novel proliferative effect of phospholipase A2 in Swiss 3T3 cells via specific binding site
    H Arita
    Shionogi Research Laboratories, Shionogi and Co, Ltd, Osaka, Japan
    J Biol Chem 266:19139-41. 1991
  4. doi Native state dynamics affects the folding transition of porcine pancreatic phospholipase A2
    Knut Kölbel
    Biomolecular and Analytical Mass Spectrometry, Chemistry Department, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium Electronic address
    Biophys Chem 206:12-21. 2015
  5. ncbi Group IB secretory phospholipase A2 stimulates leukotriene B4 production by a unique mechanism in human neutrophils
    Ha Young Lee
    Medical Research Center for Cancer Molecular Therapy, College of Medicine, Dong A University, Busan 602 714, Republic of Korea
    Biochem Biophys Res Commun 334:500-8. 2005
  6. ncbi Identification of a soluble form phospholipase A2 receptor as a circulating endogenous inhibitor for secretory phospholipase A2
    Ken ichi Higashino Ki
    Shionogi Research Laboratories, Shionogi and Co, Ltd 12 4 Sagisu, 5 Chome, Fukushima ku, Osaka 553 0002, Japan
    J Biol Chem 277:13583-8. 2002
  7. ncbi Structural comparison of phospholipase-A2-binding regions in phospholipase-A2 receptors from various mammals
    K Higashino
    Shionogi Research Laboratories, Shionogi and Co, Ltd, Osaka, Japan
    Eur J Biochem 225:375-82. 1994
  8. ncbi Structure of porcine pancreatic phospholipase A2 at 2.6 A resolution and comparison with bovine phospholipase A2
    B W Dijkstra
    J Mol Biol 168:163-79. 1983
  9. ncbi X-ray structure of phospholipase A2 complexed with a substrate-derived inhibitor
    M M Thunnissen
    Laboratory of Chemical Physics, University of Groningen, The Netherlands
    Nature 347:689-91. 1990
  10. ncbi The primary structure of phospholipase A2 from porcine pancreas. A reinvestigation
    W C Puijk
    Biochim Biophys Acta 492:254-9. 1977

Scientific Experts

  • Knut Kölbel
  • Hanna P Wacklin
  • Otto G Berg
  • Ha Young Lee
  • Diethard R L Monbaliu
  • Mahendra K Jain
  • Michal Gorovetz
  • Shi Bai
  • Ying H Pan
  • B van den Berg
  • Yoe Sik Bae
  • Eun Jin Jo
  • Ken ichi Higashino Ki
  • G H de Haas
  • Jacques M F Pirenne
  • Marcel M M Zeegers
  • Christel N T Dubuisson
  • Martine M J Crabbé
  • Bao Zhu Yu
  • Jos F van Pelt
  • Johan M J Fevery
  • H M Verheij
  • Miron Krimsky
  • Reuven Reich
  • Saul Yedgar
  • Ouri Schwob
  • Brian J Bahnson
  • R Boelens
  • R Kaptein
  • M Tessari
  • Eun Ha Shin
  • Kyoung Sun Park
  • Mi Kyoung Kim
  • Hyun Kyu Kang
  • Jong Young Kwak
  • Suk Hwan Baek
  • Jung Im Kim
  • Dae Won Park
  • Youl Nam Lee
  • Kohji Hanasaki
  • Shigeki Kamitani
  • Yasunori Yokota
  • Hitoshi Arita
  • Takashi Ono
  • R Dijkman
  • K Higashino
  • H Arita
  • B W Dijkstra
  • J Ishizaki
  • O Ohara
  • J Kishino
  • M M Thunnissen
  • K H Kalk
  • A J Slotboom
  • J Drenth
  • P De Geus
  • W C Puijk
  • L L van Deenen
  • P P Bonsen
  • J J Seilhamer
  • A G Tomasselli
  • N Dekker
  • W G Hol
  • W P Hoekstra
  • R L Heinrikson
  • I M Reardon
  • E Ab
  • E Oriaku
  • F J Kezdy
  • R Renetseder
  • W Nieuwenhuizen
  • H Zurcher-Neely
  • T L Randall
  • O P Kuipers
  • A R Peters
  • K Hanasaki
  • G de Haas
  • K Matsumoto
  • L K Johnson
  • J Hui
  • M Yamanaka
  • T Nakano
  • H Teraoka
  • O Kuipers
  • C J Van den Bergh
  • J Fisher
  • S Oka

Detail Information

Publications25

  1. pmc Solution structure of porcine pancreatic phospholipase A2
    B van den Berg
    Center for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands
    EMBO J 14:4123-31. 1995
    The lipolytic enzyme phospholipase A2 (PLA2) is involved in the degradation of high-molecular weight phospholipid aggregates in vivo...
  2. doi Thermodynamic reciprocity of the inhibitor binding to the active site and the interface binding region of IB phospholipase A2
    Otto G Berg
    Department of Molecular Evolution, Uppsala University Evolutionary Biology Center, Uppsala, Sweden
    Biochemistry 48:3209-18. 2009
    Interfacial activation of pig pancreatic IB phospholipase A(2) (PLA2) is modeled in terms of the three discrete premicellar complexes (E(i)(#), i = 1, 2, or 3) consecutively formed by the cooperative binding of a monodisperse amphiphile ..
  3. ncbi Novel proliferative effect of phospholipase A2 in Swiss 3T3 cells via specific binding site
    H Arita
    Shionogi Research Laboratories, Shionogi and Co, Ltd, Osaka, Japan
    J Biol Chem 266:19139-41. 1991
    Phospholipase A2 (PLA2), EC 3.1.1.4, which catalyzes the release of free fatty acids from the sn-2 position of glycerophospholipids, has been extensively studied from the viewpoint of eicosanoid production (Arita, H., Nakano, T...
  4. doi Native state dynamics affects the folding transition of porcine pancreatic phospholipase A2
    Knut Kölbel
    Biomolecular and Analytical Mass Spectrometry, Chemistry Department, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium Electronic address
    Biophys Chem 206:12-21. 2015
    ....
  5. ncbi Group IB secretory phospholipase A2 stimulates leukotriene B4 production by a unique mechanism in human neutrophils
    Ha Young Lee
    Medical Research Center for Cancer Molecular Therapy, College of Medicine, Dong A University, Busan 602 714, Republic of Korea
    Biochem Biophys Res Commun 334:500-8. 2005
    ..Taken together, we suggest that sPLA(2)-IB plays a role in the modulation of inflammatory and immune responses by inducing LTB4 production in human neutrophils...
  6. ncbi Identification of a soluble form phospholipase A2 receptor as a circulating endogenous inhibitor for secretory phospholipase A2
    Ken ichi Higashino Ki
    Shionogi Research Laboratories, Shionogi and Co, Ltd 12 4 Sagisu, 5 Chome, Fukushima ku, Osaka 553 0002, Japan
    J Biol Chem 277:13583-8. 2002
    ..Thus, sPLA(2)R is the first example of circulating PLI that acts as an endogenous inhibitor for enzymatic activities and receptor-mediated functions of sPLA(2)s in mice...
  7. ncbi Structural comparison of phospholipase-A2-binding regions in phospholipase-A2 receptors from various mammals
    K Higashino
    Shionogi Research Laboratories, Shionogi and Co, Ltd, Osaka, Japan
    Eur J Biochem 225:375-82. 1994
    We determined the nucleotide sequence of a mouse cDNA encoding the receptor for pancreatic group I phospholipase A2 (PLA2-I)...
  8. ncbi Structure of porcine pancreatic phospholipase A2 at 2.6 A resolution and comparison with bovine phospholipase A2
    B W Dijkstra
    J Mol Biol 168:163-79. 1983
    ..In addition to the calcium ion in the active site, a second calcium ion is present in the crystals; this is located on a crystallographic 2-fold axis and stabilizes the interaction between two neighbouring molecules...
  9. ncbi X-ray structure of phospholipase A2 complexed with a substrate-derived inhibitor
    M M Thunnissen
    Laboratory of Chemical Physics, University of Groningen, The Netherlands
    Nature 347:689-91. 1990
    ....
  10. ncbi The primary structure of phospholipase A2 from porcine pancreas. A reinvestigation
    W C Puijk
    Biochim Biophys Acta 492:254-9. 1977
    ..The structure which is presented here shows a high degree of homology with the amino acid sequence of snake venom and horse pancreas phospholipase A2...
  11. doi Increased serum phospholipase A2 activity after non-heart-beating donor liver transplantation and association with ischemia-reperfusion injury
    Diethard R L Monbaliu
    Department of Abdominal Transplant Surgery, University Hospitals Leuven, Leuven, Belgium
    J Surg Res 151:125-31. 2009
    ..A role for sPLA(2) in LTx from non-heart-beating donors (NHBD) has never been demonstrated. Furthermore data on the natural sPLA(2) inhibition capacity are scarce...
  12. doi Contiguous binding of decylsulfate on the interface-binding surface of pancreatic phospholipase A2
    Shi Bai
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 47:2899-907. 2008
    Pig pancreatic IB phospholipase A 2 (PLA2) forms three distinguishable premicellar E i (#) ( i = 1, 2, and 3) complexes at successively higher decylsulfate concentrations. The Hill coefficient for E 1 (#) is n 1 = 1...
  13. ncbi MMP production in human fibrosarcoma cells and their invasiveness are regulated by group IB secretory phospholipase A2 receptor-mediated activation of cytosolic phospholipase A2
    Michal Gorovetz
    Department of Pharmacology, Hebrew University School of Pharmacy, Hebrew University Hadassah Medical School, Jerusalem, Israel 91120
    Front Biosci 13:1917-25. 2008
    ....
  14. pmc Structural basis for bile salt inhibition of pancreatic phospholipase A2
    Ying H Pan
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    J Mol Biol 369:439-50. 2007
    ..of amphipathic bile salts have been thought to interact with and activate the enzyme group-IB phospholipase A2 (PLA2) derived from the pancreas...
  15. ncbi Distribution of reaction products in phospholipase A2 hydrolysis
    Hanna P Wacklin
    Oxford University, Physical and Theoretical Chemistry Laboratory, South Parks Road, Oxford OX1 3QZ, UK
    Biochim Biophys Acta 1768:1036-49. 2007
    ..These are discussed in terms of a model of fatty-acid mediated activation of PLA(2)...
  16. ncbi Porcine pancreatic phospholipase A2: sequence-specific 1H and 15N NMR assignments and secondary structure
    N Dekker
    Center for Biomembranes and Lipid Enzymology, State University of Utrecht, The Netherlands
    Biochemistry 30:3135-46. 1991
    ..This and the absence of several expected NOEs imply that this region has a less well defined structure in solution. Finally, the hydrogen bond between residues 78-81, which is part of a beta-sheet, does not show slow exchange behavior...
  17. ncbi Group IB secretory phospholipase A2 stimulates CXC chemokine ligand 8 production via ERK and NF-kappa B in human neutrophils
    Eun Jin Jo
    Medical Research Center for Cancer Molecular Therapy and Dong A University, Busan 602 714, Korea
    J Immunol 173:6433-9. 2004
    ..Taken together, we suggest that sPLA(2)-IB plays a role in the modulation of inflammatory and immune responses via the sPLA(2) receptor, by inducing CXCL8 in human neutrophils...
  18. ncbi Dimerization and activation of porcine pancreatic phospholipase A2 via substrate level acylation of lysine 56
    A G Tomasselli
    Biopolymer Chemistry Unit, Upjohn Company, Kalamazoo, Michigan 49001
    J Biol Chem 264:10041-7. 1989
    ..L., and Kézdy, F. J. (1988) J. Biol. Chem. 263, 11237-11241), strongly support the proposal that interfacial activation of monomeric phospholipases is due to substrate-level autoacylation resulting in fully potentiated dimeric enzymes...
  19. ncbi Structure of an engineered porcine phospholipase A2 with enhanced activity at 2.1 A resolution. Comparison with the wild-type porcine and Crotalus atrox phospholipase A2
    M M Thunnissen
    Laboratory of Chemical Physics, University of Groningen, The Netherlands
    J Mol Biol 216:425-39. 1990
    ..In the mutant structure, this hydrophobic pocket is now occupied by the disulfide bond between residues 61 and 91. A detailed description of the second binding site for a calcium ion in this enzyme is given...
  20. ncbi Solution structure of porcine pancreatic phospholipase A2 complexed with micelles and a competitive inhibitor
    B van den Berg
    Center for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands
    J Biomol NMR 5:110-21. 1995
    The three-dimensional structure of porcine pancreatic PLA2 (PLA2), present in a 40 kDa ternary complex with micelles and a competitive inhibitor, has been determined using multidimensional heteronuclear NMR spectroscopy...
  21. ncbi NMR structures of phospholipase A2 reveal conformational changes during interfacial activation
    B van den Berg
    Department of Enzymology and Protein Engineering, Utrecht University, The Netherlands
    Nat Struct Biol 2:402-6. 1995
    It has long been proposed that the higher activity of phospholipase A2 (PLA2) for substrates presented as multimolecular aggregates compared to dispersed molecules (interfacial activation) arises due to a conformational change in the ..
  22. ncbi Studies on phospholipase A and its zymogen from porcine pancreas. I. The complete amino acid sequence
    G H de Haas
    Biochim Biophys Acta 221:31-53. 1970
  23. ncbi Studies on phospholipase A and its zymogen from porcine pancreas. II. The assignment of the position of the six disulfide bridges
    G H de Haas
    Biochim Biophys Acta 221:54-61. 1970
  24. pmc Expression of porcine pancreatic phospholipase A2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein from Escherichia coli
    P De Geus
    Nucleic Acids Res 15:3743-59. 1987
    ..The released (pro)PLA was purified (yields of 2-3 mg/ltr of culture medium), and showed identical properties compared to native (pro)PLA...
  25. ncbi Pancreatic phospholipase A2: isolation of the human gene and cDNAs from porcine pancreas and human lung
    J J Seilhamer
    DNA 5:519-27. 1986
    Oligonucleotide probes synthesized using the published protein sequence for porcine pancreatic phospholipase A2 (PLA2; Puijk et al., 1977), were used to screen a cDNA library made from porcine pancreas...