Genomes and Genes
Gene Symbol: Syt1
Description: synaptotagmin I
Alias: AW124717, G630098F17Rik, SytI, synaptotagmin-1, p65
- Synaptic vesicle and synaptic membrane glycoproteins during pre- and postnatal development of mouse cerebral cortex, cerebellum and spinal cordC S Mayanil
Laboratory for Oculo Cerebrospinal Investigation, Children s Memorial Medical Center, Chicago, IL 60614
Dev Neurosci 15:133-45. 1993..The developmental expression of synaptotagmin 1, a well characterized glycoprotein of synaptic vesicle, was determined by immunoblots analysis...
- The Janus-faced nature of the C(2)B domain is fundamental for synaptotagmin-1 functionMingshan Xue
Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
Nat Struct Mol Biol 15:1160-8. 2008....
- Differential dependence of phasic transmitter release on synaptotagmin 1 at GABAergic and glutamatergic hippocampal synapsesAngharad M Kerr
Physiological Institute I, University of Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
Proc Natl Acad Sci U S A 105:15581-6. 2008Previous studies revealed that synaptotagmin 1 is the major Ca(2+) sensor for fast synchronous transmitter release at excitatory synapses...
- C-terminal ECFP fusion impairs synaptotagmin 1 function: crowding out synaptotagmin 1Weiping Han
Center for Basic Neuroscience, The Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
J Biol Chem 280:5089-100. 2005To allow the monitoring of synaptotagmin 1 trafficking in vivo, we generated transgenic mice expressing a synaptotagmin 1-enhanced cyan fluorescent protein (ECFP) fusion protein under control of the Thy1 promoter...
- Release of FGF1 and p40 synaptotagmin 1 correlates with their membrane destabilizing abilityIrene Graziani
Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074, USA
Biochem Biophys Res Commun 349:192-9. 2006..FGF1, p40 Syt1, and S100A13 induced destabilization of liposomes composed of acidic but not of zwitterionic pL...
- Alcohol induces synaptotagmin 1 expression in neurons via activation of heat shock factor 1F P Varodayan
Department of Neuroscience, Columbia University, 40 Haven Avenue, Room 865, New York, NY 10032, USA
Neuroscience 193:63-71. 2011..we investigated the effects of acute alcohol exposure (1-24 h) on the expression of the gene encoding synaptotagmin 1 (Syt1), a synaptic protein that binds calcium to directly trigger vesicle fusion...
- Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin IIM Fukuda
Molecular Neurobiology Laboratory, Tsukuba Life Science Center, Ibaraki, Japan
J Biol Chem 269:29206-11. 1994..Our data suggest that the C2B domain of synaptotogamin is the inositol polyphosphate sensor at the synaptic vesicle and may be involved in synaptic function...
- Differential but convergent functions of Ca2+ binding to synaptotagmin-1 C2 domains mediate neurotransmitter releaseOk Ho Shin
Departments of Neuroscience, Molecular Genetics, Biochemistry, and Pharmacology, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
Proc Natl Acad Sci U S A 106:16469-74. 2009....
- SV2B regulates synaptotagmin 1 by direct interactionDiana R Lazzell
WM Keck Center for Learning and Memory, Department of Neurobiology and Anatomy, University of Texas Medical School, Houston, Texas 77030, USA
J Biol Chem 279:52124-31. 2004..Previous studies found that SV2A, but not SV2B, can interact with the cytoplasmic domain of synaptotagmin 1, a Ca2+ sensor for synaptic vesicle exocytosis...
- Synaptotagmin arrests the SNARE complex before triggering fast, efficient membrane fusion in response to Ca2+Michael C Chicka
Howard Hughes Medical Institute and Department of Physiology, University of Wisconsin, Madison, 1300 University Avenue, SMI 129, Madison, Wisconsin 53706, USA
Nat Struct Mol Biol 15:827-35. 2008..These findings demonstrate that Ca(2+) converts synaptotagmin from a clamp to a trigger for exocytosis...
- Functional dissection of the interactions of stonin 2 with the adaptor complex AP-2 and synaptotagminKristin Walther
Department of Biochemistry II, Zentrum für Biochemie and Molekulare Zellbiologie, University of Gottingen, Humboldtallee 23, 37073 Gottingen, Germany
Proc Natl Acad Sci U S A 101:964-9. 2004..We hypothesize that stonin 2 may function as an AP-2-dependent sorting adaptor for synaptic vesicle recycling...
- Synaptotagmin-1 docks secretory vesicles to syntaxin-1/SNAP-25 acceptor complexesHeidi de Wit
Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, Vrije Universiteit Amsterdam and VU Medical Center, 1081 HV Amsterdam, The Netherlands
Cell 138:935-46. 2009....
- Synaptotagmins I and II act as nerve cell receptors for botulinum neurotoxin GAndreas Rummel
Institutes of Biochemistry and Toxicology, Medizinische Hochschule Hannover, D 30625 Hannover, Germany
J Biol Chem 279:30865-70. 2004..In addition, we show that the carboxyl-terminal domain of the cell binding fragment of BoNT/B and BoNT/G mediates the interaction with their protein receptor...
- Synaptotagmin I synchronizes transmitter release in mouse hippocampal neuronsTei ichi Nishiki
Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Neurosci 24:6127-32. 2004..Thus, synaptotagmin I synchronizes the rapid release of neurotransmitters after Ca2+ entry into presynaptic terminals and also appears to suppress the slower, asynchronous form of transmitter release...
- Dual roles of the C2B domain of synaptotagmin I in synchronizing Ca2+-dependent neurotransmitter releaseTei ichi Nishiki
Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Neurosci 24:8542-50. 2004....
- Kinetic efficiency of endocytosis at mammalian CNS synapses requires synaptotagmin IKarin Nicholson-Tomishima
Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 101:16648-52. 2004..basis of this coupling is poorly understood, numerous biochemical data point to a role for synaptotagmin I (SytI), an essential synaptic vesicle protein required for fast calcium-dependent exocytosis...
- Conformational switch of syntaxin-1 controls synaptic vesicle fusionStefan H Gerber
Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
Science 321:1507-10. 2008..Thus, the closed conformation of syntaxin-1 gates the initiation of the synaptic vesicle fusion reaction, which is then mediated by SNARE-complex/Munc18-1 assemblies...
- SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle primingAndrea Burgalossi
Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, D 37075 Gottingen, Germany
Neuron 68:473-87. 2010..Our analysis of α- and βSNAP deletion mutant neurons shows that the two NSF cofactors support synaptic vesicle priming by determining the availability of free SNARE components, particularly during phases of high synaptic activity...
- Synaptotagmin-1 and -7 are functionally overlapping Ca2+ sensors for exocytosis in adrenal chromaffin cellsJean Sébastien Schonn
Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
Proc Natl Acad Sci U S A 105:3998-4003. 2008....
- Synaptotagmin-1, -2, and -9: Ca(2+) sensors for fast release that specify distinct presynaptic properties in subsets of neuronsJun Xu
Department of Neuroscience, The University of Texas Southwestern Medical Center, Dallas TX 75390, USA
Neuron 54:567-81. 2007....
- Synaptotagmin I and II are present in distinct subsets of central synapsesMichael A Fox
Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA
J Comp Neurol 503:280-96. 2007b>Synaptotagmin 1 and 2 (syt 1, syt 2) are synaptic vesicle-associated membrane proteins that act as calcium sensors for fast neurotransmitter release from presynaptic nerve terminals...
- v-SNAREs control exocytosis of vesicles from priming to fusionMaria Borisovska
Department of Physiology, University of Saarland, Homburg Saar, Germany
EMBO J 24:2114-26. 2005..We demonstrate that dynamics of fusion pore dilation are regulated by v-SNAREs, indicating their action throughout exocytosis from priming to fusion of vesicles...
- Synaptotagmin isoforms couple distinct ranges of Ca2+, Ba2+, and Sr2+ concentration to SNARE-mediated membrane fusionAkhil Bhalla
Department of Physiology, University of Wisconsin, Madison, WI 53706, USA
Mol Biol Cell 16:4755-64. 2005..Our data demonstrate that different syt isoforms are specialized to sense different ranges of divalent cations and that PS is an essential effector of Ca2+.syt action...
- Autonomous function of synaptotagmin 1 in triggering synchronous release independent of asynchronous releaseAnton Maximov
Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Neuron 48:547-54. 2005..Ca(2+) triggers neurotransmitter release in at least two principal modes, synchronous and asynchronous release. Synaptotagmin 1 functions as a Ca(2+) sensor for synchronous release, but its role in asynchronous release remains unclear...
- Augmenting neurotransmitter release by enhancing the apparent Ca2+ affinity of synaptotagmin 1J S Rhee
Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, TX 7703, USA
Proc Natl Acad Sci U S A 102:18664-9. 2005b>Synaptotagmin 1 likely acts as a Ca2+ sensor in neurotransmitter release by Ca2+-binding to its two C2 domains...
- Phosphatidylinositol phosphates as co-activators of Ca2+ binding to C2 domains of synaptotagmin 1LiYi Li
Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 281:15845-52. 2006Ca2+-dependent phospholipid binding to the C2A and C2B domains of synaptotagmin 1 is thought to trigger fast neurotransmitter release, but only Ca2+ binding to the C2B domain is essential for release...
- A gain-of-function mutation in synaptotagmin-1 reveals a critical role of Ca2+-dependent soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex binding in synaptic exocytosisZhiping P Pang
Center for Basic Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
J Neurosci 26:12556-65. 2006..These data establish that fast vesicle exocytosis is driven by a dual Ca2+-dependent activity of synaptotagmin-1, namely Ca2+-dependent binding both to SNARE complexes and to phospholipids...
- A complexin/synaptotagmin 1 switch controls fast synaptic vesicle exocytosisJiong Tang
The Center for Basic Neuroscience, UT Southwestern Medical Center, Dallas, TX 75390, USA
Cell 126:1175-87. 2006Ca(2+) binding to synaptotagmin 1 triggers fast exocytosis of synaptic vesicles that have been primed for release by SNARE-complex assembly. Besides synaptotagmin 1, fast Ca(2+)-triggered exocytosis requires complexins...
- Complexin clamps asynchronous release by blocking a secondary Ca(2+) sensor via its accessory α helixXiaofei Yang
Department of Molecular and Cellular Physiology, Stanford University, 1050 Arastradero Road, Palo Alto, CA 94304 5543, USA
Neuron 68:907-20. 2010..Thus, complexin performs mechanistically distinct activation and clamping functions that operate in conjunction with synaptotagmin-1 by controlling trans-SNARE-complex assembly...
- Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagminsC Li
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235, USA
Nature 375:594-9. 1995..The same C2 domains also bind syntaxin as a function of Ca2+ but the Ca(2+)-concentration dependence of Syt I, II and V (> 200 microM) differs from that of Syt III and VII (< 10 microM).(ABSTRACT TRUNCATED AT 250 WORDS)..
- Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapseM Geppert
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235
Cell 79:717-27. 1994..We propose that synaptotagmin I is the major low affinity Ca2+ sensor mediating Ca2+ regulation of synchronous neurotransmitter release in hippocampal neurons...
- Complexin activates exocytosis of distinct secretory vesicles controlled by different synaptotagminsPeng Cao
Department of Molecular and Cellular Physiology and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305 5543, USA
J Neurosci 33:1714-27. 2013....
- Glycosylation is dispensable for sorting of synaptotagmin 1 but is critical for targeting of SV2 and synaptophysin to recycling synaptic vesiclesSung E Kwon
Howard Hughes Medical Institute, Department of Neuroscience, University of Wisconsin, Madison, Wisconsin 53706, USA
J Biol Chem 287:35658-68. 2012..For example, the three major synaptic vesicle glycoproteins, synaptotagmin 1, synaptophysin, and SV2, represent ∼30% of the total copy number of vesicle proteins...
- Uncoupling the roles of synaptotagmin I during endo- and exocytosis of synaptic vesiclesJun Yao
Howard Hughes Medical Institute and Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, USA
Nat Neurosci 15:243-9. 2011Synaptotagmin I (syt1) is required for normal rates of synaptic vesicle endo- and exocytosis...
- Synaptotagmin I functions as a calcium regulator of release probabilityR Fernandez-Chacon
Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas 75390 9111, USA
Nature 410:41-9. 2001..Therefore, Ca2+ binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse...
- Intracellular calcium dependence of large dense-core vesicle exocytosis in the absence of synaptotagmin IT Voets
Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Proc Natl Acad Sci U S A 98:11680-5. 2001..Alternatively, synaptotagmin I could function as calcium sensor for the readily releasable pool, leading to the destabilization of the pool in its absence...
- Genetic ablation of the t-SNARE SNAP-25 distinguishes mechanisms of neuroexocytosisPhilip Washbourne
Department of Neurosciences, University of New Mexico Health Science Center, Albuquerque, New Mexico 87131, USA
Nat Neurosci 5:19-26. 2002..These results demonstrate that the development of neurotransmission requires the recruitment of a specialized SNARE core complex to meet the demands of regulated exocytosis...
- Doc2 is a Ca2+ sensor required for asynchronous neurotransmitter releaseJun Yao
Howard Hughes Medical Institute and Department of Neuroscience, University of Wisconsin, Madison, WI 53706, USA
Cell 147:666-77. 2011..In summary, we propose that Doc2 is a Ca(2+) sensor that is kinetically tuned to regulate asynchronous neurotransmitter release...
- Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactionsOk Ho Shin
Center for Basic Neuroscience, Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Neuron 37:99-108. 2003Sr(2+) triggers neurotransmitter release similar to Ca(2+), but less efficiently. We now show that in synaptotagmin 1 knockout mice, the fast component of both Ca(2+)- and Sr(2+)-induced release is selectively impaired, suggesting that ..
- Synaptotagmins I and II mediate entry of botulinum neurotoxin B into cellsMin Dong
Department of Physiology, University of Wisconsin, Madison, Madison, WI 53706, USA
J Cell Biol 162:1293-303. 2003..Finally, we show that syt II fragments, in conjunction with gangliosides, neutralized BoNT/B in intact mice. These findings establish that syts I and II can function as protein receptors for BoNT/B...
- Structure/function analysis of Ca2+ binding to the C2A domain of synaptotagmin 1Rafael Fernandez-Chacon
Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
J Neurosci 22:8438-46. 2002b>Synaptotagmin 1, a Ca2+ sensor for fast synaptic vesicle exocytosis, contains two C2 domains that form Ca2+-dependent complexes with phospholipids...
- SNAP-25 and synaptotagmin 1 function in Ca2+-dependent reversible docking of granules to the plasma membraneEvelina Chieregatti
Department of Anatomy and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA
Traffic 3:496-511. 2002..In the docking assay, addition of Ca2+ induces the formation of a SNAP-25-Synaptotagmin 1 complex...
- Examining synaptotagmin 1 function in dense core vesicle exocytosis under direct control of Ca2+Jakob B Sørensen
Max Planck Institut fur biophysikalische Chemie, Abteilung Membranbiophysik, Am Fassberg 11, D 37077 Gottingen, Germany
J Gen Physiol 122:265-76. 2003We tested the long-standing hypothesis that synaptotagmin 1 is the Ca2+ sensor for fast neurosecretion by analyzing the intracellular Ca2+ dependence of large dense-core vesicle exocytosis in a mouse strain carrying a mutated ..
- Different effects on fast exocytosis induced by synaptotagmin 1 and 2 isoforms and abundance but not by phosphorylationGabor Nagy
Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
J Neurosci 26:632-43. 2006Synaptotagmins comprise a large protein family, of which synaptotagmin 1 (Syt1) is a Ca2+ sensor for fast exocytosis, and its close relative, synaptotagmin 2 (Syt2), is assumed to serve similar functions...
- The alternative translation of synaptotagmin 1 mediates the non-classical release of FGF1C Bagala
Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME 04074, USA
Biochem Biophys Res Commun 310:1041-7. 2003..FGF1, it was not possible to identify a specific intracellular protease responsible for the processing of p65 Syt1. Surprisingly, analysis of the p65 Syt1 coding sequence revealed the presence of two potential alternative ATG ..
- Somatodendritic dopamine release requires synaptotagmin 4 and 7 and the participation of voltage-gated calcium channelsJose Alfredo Mendez
Department of Pharmacology, Groupe de Recherche sur le Système Nerveux Central, Faculty of Medicine, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
J Biol Chem 286:23928-37. 2011..of the dendritically expressed Syt4 and Syt7 severely reduced STD DA release, whereas terminal release required Syt1. Moreover, we found that although mobilization of intracellular Ca(2+) stores is inefficient, Ca(2+) influx through ..
- Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosisEllen Reisinger
Molecular Biology of Cochlear Neurotransmission, Department of Otolaryngology, University Medical Center Gottingen, 37099 Gottingen, Germany
J Neurosci 31:4886-95. 2011..serve as a Ca(2+) sensor in vesicle fusion in IHCs that seem to lack the classical neuronal Ca(2+) sensors synaptotagmin 1 (Syt1) and Syt2...
- Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machineryChaofeng Han
Institute of Immunology, Tsinghua University School of Medicine, Beijing, People s Republic of China
J Immunol 182:2986-96. 2009..Our findings suggest that hSCAMP5, in cooperation with the SNARE machinery, is involved in calcium-regulated exocytosis of signal peptide-containing cytokines...
- SNAREpin assembly by Munc18-1 requires previous vesicle docking by synaptotagmin 1Daniel Parisotto
Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Biol Chem 287:31041-9. 2012..docking, priming, and fusion of small unilamellar vesicles containing the v-SNARE VAMP2 and the Ca(2+) sensor synaptotagmin 1. In vitro assays allowed us to position Munc18-1 in the center of a sequential reaction cascade; vesicle ..
- Synaptotagmin-1 functions as a Ca2+ sensor for spontaneous releaseJun Xu
Department of Molecular and Cellular Physiology, Stanford University, Palo Alto, California, USA
Nat Neurosci 12:759-66. 2009..that >95% of spontaneous release in murine cortical neurons was induced by Ca2+-binding to synaptotagmin-1 (Syt1), the Ca2+ sensor for fast synchronous neurotransmitter release...
- Synaptic protein and pan-neuronal gene expression and their regulation by Dicer-dependent mechanisms differ between neurons and neuroendocrine cellsJutta Stubbusch
Max Planck Institute for Brain Research, Deutschordenstrasse 46 D 60528, Frankfurt, Germany
Neural Dev 8:16. 2013....
- Synaptotagmin-1 and synaptotagmin-7 trigger synchronous and asynchronous phases of neurotransmitter releaseTaulant Bacaj
Department of Molecular and Cellular Physiology and Howard Hughes Medical Institute, Stanford University Medical School, Stanford, CA 94305, USA
Neuron 80:947-59. 2013....
- Phosphorylation of synaptic vesicle protein 2A at Thr84 by casein kinase 1 family kinases controls the specific retrieval of synaptotagmin-1Ning Zhang
Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom, and
J Neurosci 35:2492-507. 2015....
- Phosphorylation of synaptotagmin-1 controls a post-priming step in PKC-dependent presynaptic plasticityArthur P H de Jong
Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University and VU Medical Center, Amsterdam 1081HV, The Netherlands
Proc Natl Acad Sci U S A 113:5095-100. 2016..Here, we show that a mutation in synaptotagmin-1 (Syt1(T112A)), which prevents its PKC-dependent phosphorylation, abolishes DAG-induced potentiation of synaptic ..
- Architecture of the synaptotagmin-SNARE machinery for neuronal exocytosisQiangjun Zhou
Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, USA
Nature 525:62-7. 2015....
- Identification of the novel activity-driven interaction between synaptotagmin 1 and presenilin 1 links calcium, synapse, and amyloid betaAkira Kuzuya
MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, 02129, USA
BMC Biol 14:25. 2016....
- The secretory granule-associated protein CAPS2 regulates neurotrophin release and cell survivalTetsushi Sadakata
Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama 351 0198, Japan
J Neurosci 24:43-52. 2004..Thus, we suggest that CAPS2 mediates the depolarization-dependent release of NT-3 and BDNF from granule cells, leading to regulation in cell differentiation and survival during cerebellar development...
- Scrg1, a novel protein of the CNS is targeted to the large dense-core vesicles in neuronal cellsFrançoise Dandoy-Dron
Laboratoire d Oncologie virale, CNRS UPR 9045, 7 rue G Môquet BP 8, 94801 Villejuif Cedex, France
Eur J Neurosci 18:2449-59. 2003..Our findings strongly suggest that Scrg1 is associated with the secretory pathway of neuronal cells...
- Synaptotagmin 1 is necessary for the Ca2+ dependence of clathrin-mediated endocytosisLi Hua Yao
Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607, USA
J Neurosci 32:3778-85. 2012..that the dynamics of vesicle fission during CME is Ca²⁺ dependent but becomes Ca²⁺ independent in synaptotagmin 1 (Syt1) knock-out cells. Our results thus suggest that Syt1 is necessary for the Ca²⁺ dependence of CME.
- Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytesXiu Lan Zhu
Center for Reproductive Medicine, Department of Ob Gy, Nanfang Hospital, Southern Medical University, Guangzhou City, Guangdong Province, China
Cell Cycle 11:818-26. 2012..Here, we identified the unique localization, expression and function of Syt1 during mouse oocyte meiotic maturation by using confocal microscopy, western blotting, Morpholino-based knockdown ..
- Reconstituted synaptotagmin I mediates vesicle docking, priming, and fusionZhao Wang
Department of Neuroscience, Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706, USA
J Cell Biol 195:1159-70. 2011..Together, these findings reveal further convergence of reconstituted and cell-based systems...
- DJ-1 associates with synaptic membranesYukiko Usami
Department of Neurology, Juntendo University School of Medicine, Japan
Neurobiol Dis 43:651-62. 2011..These results indicate that DJ-1 associates with membranous organelles including synaptic membranes to exhibit its normal function...
- Crystal structure of the botulinum neurotoxin type G binding domain: insight into cell surface bindingPal Stenmark
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
J Mol Biol 397:1287-97. 2010..While BoNT/G and BoNT/B are homologous to each other and both utilize Syt-I/Syt-II as their protein receptor, the precise means by which these two toxin serotypes bind to Syt appears surprisingly divergent...
- Synaptotagmin 3 deficiency in T cells impairs recycling of the chemokine receptor CXCR4 and thereby inhibits CXCL12 chemokine-induced migrationAgnieszka Masztalerz
Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066CX Amsterdam, The Netherlands
J Cell Sci 120:219-28. 2007..Migration was restored by overexpression of CXCR4. We conclude that STT3 is essential for CXCR4 recycling in T cells and thereby for the maintenance of high CXCR4 surface levels required for migration...
- RIM1alpha and interacting proteins involved in presynaptic plasticity mediate prepulse inhibition and additional behaviors linked to schizophreniaJacqueline Blundell
Departments of Neurology and Psychiatry, The University of Texas Southwestern Medical Center, Dallas, Texas, 75390 8813, USA
J Neurosci 30:5326-33. 2010..selective to RIM1alpha-deficient mice, as mice bearing mutations in the RIM1alpha binding partners Rab3A or synaptotagmin 1 only show decreased prepulse inhibition...
- Cotrafficking of SV2 and synaptotagmin at the synapseJia Yao
Department of Pharmacology, University of Washington, Seattle, Washington 98195, USA
J Neurosci 30:5569-78. 2010....
- Structural and mutational analysis of functional differentiation between synaptotagmins-1 and -7Mingshan Xue
Department of Neuroscience, Baylor College of Medicine, Houston, Texas, United States of America
PLoS ONE 5:. 2010..chimera that contains the synaptotagmin-1 sequence with its C2B domain replaced by the synaptotagmin-7 C2B domain (Syt1/7)...
- Control of exocytosis by synaptotagmins and otoferlin in auditory hair cellsMaryline Beurg
Equipe Neurophysiologie de la Synapse Auditive, Institut National de la Sante et de la Recherche Medicale, Unité Mixte de Récherche Scientifique 587, Université Victor Segalen and Institut des Neurosciences de Bordeaux, Centre Hospitalier Universitaire Pellegrin, 33076 Bordeaux, France
J Neurosci 30:13281-90. 2010..During this early exocytotic period, several synaptotagmins (Syts), including Syt1, Syt2 and Syt7, were detected in IHCs...
- Synaptotagmin1 synthesis induced by synaptic plasticity in mouse hippocampus through activation of nicotinic acetylcholine receptorsTakaaki Nishimoto
Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University, 7 2 1 Kamiohno, Himeji 670 8524, Japan
Neurosci Lett 489:25-9. 2011..These results suggest that new-synthesized synaptotagmin1 following synaptic plasticity may contribute to long-lasting synaptic plasticity via positive, feedfoward mechanisms...
- TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connectionsA Martinez
Department of Animal and Plant Cell Biology, University of Barcelona, Barcelona 08028, Spain
J Neurosci 18:7336-50. 1998..The data also suggest that the genes encoding for synaptic proteins may be targets of TrkB and TrkC signaling pathways...
- Synaptotagmins: C2-domain proteins that regulate membrane trafficT C Sudhof
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235, USA
Neuron 17:379-88. 1996
- Genetic mapping of five mouse genes encoding synaptotagminsO J Kwon
Laboratory of Neurochemistry, NINDS, National Institutes of Health, Bethesda, Maryland 20892, USA
Mamm Genome 6:880-1. 1995
- Synaptobrevin is essential for secretion but not for the development of synaptic processesG Ahnert-Hilger
Medizinische Klinik und Poliklinik, Abt Gastroenterologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Germany
Eur J Cell Biol 70:1-11. 1996..We conclude that synaptobrevin represents a marker for early events in synaptogenesis. Its proteolysis by tetanus toxin, however, does not interfere with the formation of synaptic contacts and neuronal differentiation...
- Synaptotagmin genes on mouse chromosomes 1, 7, and 10 and human chromosome 19J M Jones
Department of Human Genetics, University of Michigan, Ann Arbor 48109 0618, USA
Mamm Genome 6:212-3. 1995
- Insertional mutation of the motor endplate disease (med) locus on mouse chromosome 15D C Kohrman
Department of Human Genetics, University of Michigan, Ann Arbor 48109 0618, USA
Genomics 26:171-7. 1995..The homologous human locus MED was assigned to chromosome 12. Synaptotagmin 1 and contactin 1 were eliminated as candidate genes for the med mutation...
- Cyclic AMP-mediated endocytosis of intestinal epithelial NHE3 requires binding to synaptotagmin 1Mark W Musch
Martin Boyer Laboratories, Department of Medicine, University of Chicago, 5841 S Maryland Ave, Chicago, IL 60637, USA
Am J Physiol Gastrointest Liver Physiol 298:G203-11. 2010..The clathrin complex adaptor protein synaptotagmin 1 (Syt 1) appears to be essential to this process, but little is known about its expression in intestinal ..
- Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reactionDarren M Hutt
Ottawa Health Research Institute, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario K1Y 4E9, Canada
J Biol Chem 280:20197-203. 2005..Taken together, our data suggest a critical role for Syt VIII and Stx2 in membrane fusion and acrosome reaction in the sperm...
- Upregulation of hippocampal TrkB and synaptotagmin is involved in treadmill exercise-enhanced aversive memory in miceYu Fan Liu
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan
Neurobiol Learn Mem 90:81-9. 2008..Taken together, these data suggest that the upregulation of TrkB and synaptotagmin in the hippocampus contributes to the exercise-facilitated aversive memory...
- Synaptotagmin IV regulates dense core vesicle (DCV) release in LbetaT2 cellsZhi Tao Hu
Key Laboratory of Molecular Biophysics, Ministry of Education, and Joint Laboratory of Institute of Biophysics and Huazhong University of Science and Technology, Huazhong University of Science and Technology, Luoyu Road 1037, Wuhan 430074, PR China
Biochem Biophys Res Commun 371:781-6. 2008..Downregulation of Syt IV clearly reduced Ca(2+)-triggered exocytosis of dense core vesicles (DCVs) in LbetaT2 cells. Syt I silencing, however, had no effect on vesicular release...
- Breakdown of axonal synaptic vesicle precursor transport by microglial nitric oxideMassimiliano Stagi
Neuroimmunology Group, European Neuroscience Institute Göttingen, 37073 Gottingen, Germany
J Neurosci 25:352-62. 2005..Thus, overt production of reactive NO by activated microglia blocks the axonal transport of synaptic vesicle precursors via phosphorylation of JNK and could cause axonal and synaptic dysfunction...
- Mechanism of botulinum neurotoxin B and G entry into hippocampal neuronsMin Dong
Howard Hughes Medical Institute, Department of Physiology, University of Wisconsin, Madison, WI 53706, USA
J Cell Biol 179:1511-22. 2007..These data suggest that gangliosides are the shared coreceptor for BoNT/A, B, and G, supporting a double-receptor model for these three BoNTs for which the protein receptors are known...
- WNK1 phosphorylates synaptotagmin 2 and modulates its membrane bindingByung Hoon Lee
Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Mol Cell 15:741-51. 2004..These findings provide a biochemical mechanism that could lead to the retention or insertion of proteins in the plasma membrane. Interruption of this regulatory pathway may disturb membrane events that regulate ion balance...
- Role of topoisomerase IIbeta in the expression of developmentally regulated genesYi Lisa Lyu
Department of Pharmacology, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Mol Cell Biol 26:7929-41. 2006..Together, these results support a role of TopIIbeta in activation/repression of developmentally regulated genes at late stages of neuronal differentiation...
- Normal development and fertility of knockout mice lacking the tumor suppressor gene LRP1b suggest functional compensation by LRP1Peter Marschang
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Mol Cell Biol 24:3782-93. 2004..Analysis of LRP1b knockout mice may provide further insights into the role of LRP1b as a tumor suppressor and into the mechanisms of cancer development...
- Differential effects of treadmill running and wheel running on spatial or aversive learning and memory: roles of amygdalar brain-derived neurotrophic factor and synaptotagmin IYu Fan Liu
Institute of Basic Medical Sciences, Department of Physiology, National Cheng Kung University, Tainan, Taiwan
J Physiol 587:3221-31. 2009..Therefore, our results support the notion that different forms of exercise induce neuroplasticity changes in different brain regions, and thus exert diverse effects on various forms of learning and memory...
- Involvement of Ca2+ channel synprint site in synaptic vesicle endocytosisHiroyasu Watanabe
Cellular and Molecular Synaptic Function Unit, Initial Research Project, Okinawa Institute of Science and Technology Promotion Corporation, Okinawa 904 2234, Japan
J Neurosci 30:655-60. 2010..bind the mu subunit of AP-2, an adaptor protein for clathrin-mediated endocytosis, in competition with the synaptotagmin 1 (Syt 1) C2B domain...
- The presynaptic active zone protein RIM1alpha is critical for normal learning and memoryCraig M Powell
Department of Psychiatry, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Neuron 42:143-53. 2004..Mice with a synaptotagmin 1 mutation, which selectively lowers release probability, and mice with Rab3A deletion, which selectively ..
- Synaptotagmin mutants Y311N and K326/327A alter the calcium dependence of neurotransmissionCatherine R Borden
Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Mol Cell Neurosci 29:462-70. 2005..We conclude that self-oligomerization is unlikely to play any significant role in triggering synaptic vesicle exocytosis...
- A third synaptotagmin gene, Syt3, in the mouseB S Hilbush
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110
Proc Natl Acad Sci U S A 91:8195-9. 1994..In the most highly conserved C2 domain, the mammalian synaptotagmins, SYT1 and SYT2, share 88% sequence identity, whereas SYT3 has only approximately 45% identity to either...
- Defective neuromuscular synaptogenesis in agrin-deficient mutant miceM Gautam
Department of Anatomy and Neurobiology, Washington University Medical School St Louis, Missouri 63110, USA
Cell 85:525-35. 1996....
- CAPS1 regulates catecholamine loading of large dense-core vesiclesDina Speidel
Max Planck Institut fur experimentelle Medizin, Abteilung Molekulare Neurobiologie, D 37075 Gottingen, Germany
Neuron 46:75-88. 2005..We conclude that CAPS1 is required for an essential step in the uptake or storage of catecholamines in LDCVs...
- Complexins: cytosolic proteins that regulate SNAP receptor functionH T McMahon
Howard Hughes Medical Institute, University of Texas Southwestern Medical School, Dallas 75235, USA
Cell 83:111-9. 1995....
- Synapsin and synaptic vesicle protein expression during embryonic and post-natal lens fiber cell differentiationPeter H Frederikse
Department of Pharmacology and Physiology, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
Mol Vis 10:794-804. 2004..In the present study we examine lens expression and regulation of a complement of genes associated with cargo and synaptic vesicle transport in neurons...
- Botulinum neurotoxin A activity is dependent upon the presence of specific gangliosides in neuroblastoma cells expressing synaptotagmin IBrian C Yowler
Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
J Biol Chem 277:32815-9. 2002....
- Calmodulin and lipid binding to synaptobrevin regulates calcium-dependent exocytosisStephanie Quetglas
Institut National de la Santé et de la Recherche Médicale Unité 464, Universite de la Mediterranee, 13916 Marseille Cedex 20, France
EMBO J 21:3970-9. 2002..The calmodulin- and phospholipid-binding domain of VAMP 2 is thus required for Ca(2+)-dependent exocytosis, possibly to regulate SNARE complex assembly...
- Molecular cloning, expression, and characterization of a novel class of synaptotagmin (Syt XIV) conserved from Drosophila to humansMitsunori Fukuda
Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
J Biochem 133:641-9. 2003....
- Molecular determinants of synapsin targeting to presynaptic terminalsDaniel Gitler
Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Neurosci 24:3711-20. 2004..Domain D inhibited targeting, but this inhibition was overcome by domain E. Thus, multiple intermolecular and intramolecular interactions are required for synapsins to target to presynaptic terminals...
- Morphogenesis of the telencephalic commissure requires scaffold protein JNK-interacting protein 3 (JIP3)Nyaya Kelkar
Howard Hughes Medical Institute and Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 100:9843-8. 2003..The central nervous system abnormalities of Jip3-/- mice may be accounted for in part by a reduction in signal transduction by RhoA and its effector ROCK...
- Synaptotagmin IV regulates glial glutamate releaseQi Zhang
Department of Neuroscience, Room 215, Stemmler Hall, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 101:9441-6. 2004....
- Quantification of synapse formation and maintenance in vivo in the absence of synaptic releaseJ Bouwman
Rudolf Magnus Institute for Neurosciences, University of Utrecht Medical Center, Utrecht, The Netherlands
Neuroscience 126:115-26. 2004..These two observations suggest that secretion of neurotransmitters and neuromodulatory substances is required for synapse maintenance, not for synaptogenesis. Multivesicular structures may develop out of unstable synapses...
- ALZHEIMER PATHOPHYSIOLOGY IN HUMAN CATARACT FORMATIONPETER FREDERIKSE; Fiscal Year: 2002..abstract_text> ..
- BDNF in depression, antidepressant action, and memoryCRAIG POWELL; Fiscal Year: 2007..We also propose to make use of focal injection of Cre-expressing AAV vectors into flox-BDNF mice to assess the specific brain subregions involved with greater temporal specificity. [unreadable] [unreadable]..
- Alzheimer disease biomarkers in lensPeter H Frederikse; Fiscal Year: 2010..These studies build on our earlier studies to understand the role of AbetaPP and Abeta in the lens which are already providing a new and specific conceptual basis for understanding cataract and its links with the brain. ..