Soat2

Summary

Gene Symbol: Soat2
Description: sterol O-acyltransferase 2
Alias: ACAT2, D15Wsu97e, sterol O-acyltransferase 2, ACAT-2, acyl-CoA:cholesterol acyltransferase, acyl-coenzyme A:cholesterol acyltransferase 2, cholesterol acyltransferase 2
Species: mouse

Top Publications

  1. ncbi Resistance to diet-induced hypercholesterolemia and gallstone formation in ACAT2-deficient mice
    K K Buhman
    Gladstone Institute of Cardiovascular Disease, P O Box 419100, San Francisco, California 94141 9100, USA
    Nat Med 6:1341-7. 2000
  2. pmc Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice
    Emily L Willner
    Gladstone Institute of Cardiovascular Disease, P O Box 419100, San Francisco, CA 94141 9100, USA
    Proc Natl Acad Sci U S A 100:1262-7. 2003
  3. ncbi Plasma cholesteryl esters provided by lecithin:cholesterol acyltransferase and acyl-coenzyme a:cholesterol acyltransferase 2 have opposite atherosclerotic potential
    Richard G Lee
    Arteriosclerosis Research Program, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Circ Res 95:998-1004. 2004
  4. ncbi ACAT2 deficiency limits cholesterol absorption in the cholesterol-fed mouse: impact on hepatic cholesterol homeostasis
    Joyce J Repa
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 8887, USA
    Hepatology 40:1088-97. 2004
  5. ncbi ACAT2 contributes cholesteryl esters to newly secreted VLDL, whereas LCAT adds cholesteryl ester to LDL in mice
    Richard G Lee
    Arteriosclerosis Research Program, Department of Pathology, Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 46:1205-12. 2005
  6. ncbi Intestinal cholesterol absorption is substantially reduced in mice deficient in both ABCA1 and ACAT2
    Ryan E Temel
    Department of Pathology, Section on Lipid Sciences, Wake Forest University Health Sciences, Winston Salem, NC, USA
    J Lipid Res 46:2423-31. 2005
  7. ncbi Dietary fat-induced alterations in atherosclerosis are abolished by ACAT2-deficiency in ApoB100 only, LDLr-/- mice
    Thomas A Bell
    Wake Forest University School of Medicine, Department of Pathology Lipid Sciences, Medical Center Blvd, Winston Salem, NC 27157, USA
    Arterioscler Thromb Vasc Biol 27:1396-402. 2007
  8. pmc Targeted depletion of hepatic ACAT2-driven cholesterol esterification reveals a non-biliary route for fecal neutral sterol loss
    J Mark Brown
    Department of Pathology, Biochemistry, and Orthopedic Surgery, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1040, USA
    J Biol Chem 283:10522-34. 2008
  9. pmc LDL particle core enrichment in cholesteryl oleate increases proteoglycan binding and promotes atherosclerosis
    John T Melchior
    Department of Pathology, Section of Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 54:2495-503. 2013
  10. pmc Inhibition of acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) prevents dietary cholesterol-associated steatosis by enhancing hepatic triglyceride mobilization
    Heather M Alger
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1040, USA
    J Biol Chem 285:14267-74. 2010

Scientific Experts

  • Adam M Lopez
  • Stephen D Turley
  • Ryan E Temel
  • Lawrence L Rudel
  • Janet K Sawyer
  • Martha D Wilson
  • Kathryn L Kelley
  • J Mark Brown
  • Matthew A Davis
  • Jun Zhang
  • Richard G Lee
  • John T Melchior
  • Stephanie M Marshall
  • Tam M Nguyen
  • Ramesh Shah
  • Robert V Farese
  • Ta Yuan Chang
  • Maximillian A Rogers
  • Kathryn Kelley
  • Heather M Alger
  • Wei Yang
  • Manya Warrier
  • Thomas A Bell
  • Jahangir Iqbal
  • Bo Liang Li
  • Bao Liang Song
  • Chang Xie
  • Catherine C Y Chang
  • Rosanne M Crooke
  • Mark J Graham
  • Elena Y Bryleva
  • John S Parks
  • Joyce J Repa
  • Kimberly K Buhman
  • Jin Zhang
  • Xiaolong Liu
  • Lijuan Wang
  • Lunyi Li
  • Ying Xiong
  • Emily L Willner
  • Kanwardeep Bura
  • Xiangbo Meng
  • Ti Zhang
  • Xiaojun Zheng
  • Penghui Zhou
  • Chenguang Xu
  • Jing Wang
  • Yibing Bai
  • Chenqi Xu
  • Chengsong Yan
  • Shuokai Chen
  • Wanli Liu
  • Shao Cong Sun
  • Catharine C Y Chang
  • Kerry M Link
  • John D Olson
  • Chi Liang Eric Yen
  • M Mahmood Hussain
  • Mohamed Boutjdir
  • Anthony D Gromovsky
  • Roy R Hantgan
  • Na Li
  • Elena Bryleva
  • Jay Liu
  • Boris L Vaisman
  • Li Juan Wang
  • David Shapiro
  • A Wayne Meikle
  • Mark M Kushnir
  • Zhang Sen Zhou
  • Alan T Remaley
  • Yong Jian Wang
  • Yan Bian
  • Carol R Kent
  • Alan L Rockwood
  • Thomas A Spencer
  • Nabil G Seidah
  • Frank M LaFerla
  • Floyd Buen
  • Brent T Harris
  • Mark C Willingham
  • Estelle Rousselet
  • K K Buhman
  • William F Hickey
  • Salvatore Oddo
  • S Novak
  • R V Farese
  • Thomas L Smith
  • J Zhong
  • S Cases

Detail Information

Publications28

  1. ncbi Resistance to diet-induced hypercholesterolemia and gallstone formation in ACAT2-deficient mice
    K K Buhman
    Gladstone Institute of Cardiovascular Disease, P O Box 419100, San Francisco, California 94141 9100, USA
    Nat Med 6:1341-7. 2000
    ..We now demonstrate that ACAT2 is the major ACAT in mouse small intestine and liver, and suggest that ACAT2 deficiency has profound effects on ..
  2. pmc Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice
    Emily L Willner
    Gladstone Institute of Cardiovascular Disease, P O Box 419100, San Francisco, CA 94141 9100, USA
    Proc Natl Acad Sci U S A 100:1262-7. 2003
    Deficiency of acyl CoA:cholesterol acyltransferase 2 (ACAT2) in mice results in a reduction in cholesterol ester synthesis in the small intestine and liver, which in turn limits intestinal cholesterol absorption, hepatic cholesterol ..
  3. ncbi Plasma cholesteryl esters provided by lecithin:cholesterol acyltransferase and acyl-coenzyme a:cholesterol acyltransferase 2 have opposite atherosclerotic potential
    Richard G Lee
    Arteriosclerosis Research Program, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Circ Res 95:998-1004. 2004
    Evidence suggests that ACAT2 is a proatherogenic enzyme that contributes cholesteryl esters (CEs) to apoB-containing lipoproteins, whereas LCAT is an antiatherogenic enzyme that facilitates reverse cholesterol transport by esterifying ..
  4. ncbi ACAT2 deficiency limits cholesterol absorption in the cholesterol-fed mouse: impact on hepatic cholesterol homeostasis
    Joyce J Repa
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 8887, USA
    Hepatology 40:1088-97. 2004
    ..Male ACAT2(+/+) and ACAT2(-/ -) mice were fed chow containing added cholesterol (0%-0.500% w/w) for 24 days...
  5. ncbi ACAT2 contributes cholesteryl esters to newly secreted VLDL, whereas LCAT adds cholesteryl ester to LDL in mice
    Richard G Lee
    Arteriosclerosis Research Program, Department of Pathology, Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 46:1205-12. 2005
    The relative contributions of ACAT2 and LCAT to the cholesteryl ester (CE) content of VLDL and LDL were measured. ACAT2 deficiency led to a significant decrease in the percentage of CE (37.2 +/- 2.1% vs. 3.9 +/- 0...
  6. ncbi Intestinal cholesterol absorption is substantially reduced in mice deficient in both ABCA1 and ACAT2
    Ryan E Temel
    Department of Pathology, Section on Lipid Sciences, Wake Forest University Health Sciences, Winston Salem, NC, USA
    J Lipid Res 46:2423-31. 2005
    ..Because of its ability to esterify cholesterol for packaging into nascent chylomicrons, ACAT2 plays an important role in cholesterol absorption...
  7. ncbi Dietary fat-induced alterations in atherosclerosis are abolished by ACAT2-deficiency in ApoB100 only, LDLr-/- mice
    Thomas A Bell
    Wake Forest University School of Medicine, Department of Pathology Lipid Sciences, Medical Center Blvd, Winston Salem, NC 27157, USA
    Arterioscler Thromb Vasc Biol 27:1396-402. 2007
    The enzyme acyl-coenzymeA (CoA):cholesterol O-acyltransferase 2 (ACAT2) in the liver synthesizes cholesteryl esters (CE) from cholesterol and fatty acyl-CoA, which get incorporated into apoB-containing lipoproteins that are secreted into ..
  8. pmc Targeted depletion of hepatic ACAT2-driven cholesterol esterification reveals a non-biliary route for fecal neutral sterol loss
    J Mark Brown
    Department of Pathology, Biochemistry, and Orthopedic Surgery, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1040, USA
    J Biol Chem 283:10522-34. 2008
    Deletion of acyl-CoA:cholesterol O-acyltransferase 2 (ACAT2) in mice results in resistance to diet-induced hypercholesterolemia and protection against atherosclerosis...
  9. pmc LDL particle core enrichment in cholesteryl oleate increases proteoglycan binding and promotes atherosclerosis
    John T Melchior
    Department of Pathology, Section of Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 54:2495-503. 2013
    ..Diet enrichment with MUFAs enhances LDL CO content. Steroyl O-acyltransferase 2 (SOAT2) is the enzyme that catalyzes the synthesis of much of the CO found in LDL, and gene deletion of SOAT2 minimizes ..
  10. pmc Inhibition of acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) prevents dietary cholesterol-associated steatosis by enhancing hepatic triglyceride mobilization
    Heather M Alger
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1040, USA
    J Biol Chem 285:14267-74. 2010
    Acyl-CoA:cholesterol O-acyl transferase 2 (ACAT2) promotes cholesterol absorption by the intestine and the secretion of cholesteryl ester-enriched very low density lipoproteins by the liver...
  11. pmc Tissue-specific knockouts of ACAT2 reveal that intestinal depletion is sufficient to prevent diet-induced cholesterol accumulation in the liver and blood
    Jun Zhang
    Section on Lipid Sciences, Department of Pathology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Lipid Res 53:1144-52. 2012
    Acyl-CoA:cholesterol acyltransferase 2 (ACAT2) generates cholesterol esters (CE) for packaging into newly synthesized lipoproteins and thus is a major determinant of blood cholesterol levels...
  12. pmc Cholesterol esterification by ACAT2 is essential for efficient intestinal cholesterol absorption: evidence from thoracic lymph duct cannulation
    Tam M Nguyen
    Department of Pathology, Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 53:95-104. 2012
    The hypothesis tested in this study was that cholesterol esterification by ACAT2 would increase cholesterol absorption efficiency by providing cholesteryl ester (CE) for incorporation into chylomicrons...
  13. pmc Targeted Knockdown of Hepatic SOAT2 With Antisense Oligonucleotides Stabilizes Atherosclerotic Plaque in ApoB100-only LDLr-/- Mice
    John T Melchior
    From the Department of Pathology, Section on Lipid Sciences J T M, K L K, M D W, J K S, L L R, and Department of Radiology, Center for Biomolecular Imaging J D O, K M L, Wake Forest University Health Sciences, Winston Salem, NC
    Arterioscler Thromb Vasc Biol 35:1920-7. 2015
    ..To test the hypothesis that the attenuation of cholesterol oleate packaging into apoB-containing lipoproteins will arrest progression of pre-existing atherosclerotic lesions...
  14. pmc Cholesterol esters (CE) derived from hepatic sterol O-acyltransferase 2 (SOAT2) are associated with more atherosclerosis than CE from intestinal SOAT2
    Jun Zhang
    From the Section on Molecular Medicine, Department of Internal Medicine, Wake Forest University School of Medicine, Winston Salem, NC J Z, J K S, S M M, K L K, M A D, M D W, L L R and Department of Cellular and Molecular Medicine, Cleveland Clinic Lerner Research Institute, OH S M M, J M B
    Circ Res 115:826-33. 2014
    ..esters (CE), especially cholesterol oleate, generated by hepatic and intestinal sterol O-acyltransferase 2 (SOAT2) play a critical role in cholesterol homeostasis...
  15. pmc Intestine-specific MTP and global ACAT2 deficiency lowers acute cholesterol absorption with chylomicrons and HDLs
    Jahangir Iqbal
    Departments of Cell Biology and Pediatrics, SUNY Downstate Medical Center, Brooklyn, NY 11203 Veterans Affairs New York Harbor Healthcare System, Brooklyn, NY 11209
    J Lipid Res 55:2261-75. 2014
    ..Chylomicrons transport free and esterified cholesterol, whereas HDLs transport free cholesterol. ACAT2 esterifies cholesterol for secretion with chylomicrons...
  16. pmc Acute sterol o-acyltransferase 2 (SOAT2) knockdown rapidly mobilizes hepatic cholesterol for fecal excretion
    Stephanie M Marshall
    Department of Pathology, Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, North Carolina, United States of America
    PLoS ONE 9:e98953. 2014
    ..previously showed that chronic knockdown of the hepatic cholesterol esterifying enzyme sterol O-acyltransferase 2 (SOAT2) increased fecal cholesterol loss via TICE...
  17. pmc Deletion of sterol O-acyltransferase 2 (SOAT2) function in mice deficient in lysosomal acid lipase (LAL) dramatically reduces esterified cholesterol sequestration in the small intestine and liver
    Adam M Lopez
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 9151, United States Electronic address
    Biochem Biophys Res Commun 454:162-6. 2014
    Sterol O-acyltransferase 2 (SOAT2), also known as ACAT2, is the major cholesterol esterifying enzyme in the liver and small intestine (SI)...
  18. ncbi Compared with Acyl-CoA:cholesterol O-acyltransferase (ACAT) 1 and lecithin:cholesterol acyltransferase, ACAT2 displays the greatest capacity to differentiate cholesterol from sitosterol
    Ryan E Temel
    Department of Pathology, Section on Comparative Medicine, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 278:47594-601. 2003
    ..Cholesterol-loaded microsomes from transfected cells containing either ACAT1 or ACAT2 exhibited significantly more ACAT activity than their sitosterol-loaded counterparts...
  19. pmc Sterol O-Acyltransferase 2-Driven Cholesterol Esterification Opposes Liver X Receptor-Stimulated Fecal Neutral Sterol Loss
    Manya Warrier
    Department of Cellular and Molecular Medicine, Cleveland Clinic Lerner Research Institute, Cleveland, OH, 44195, USA
    Lipids 51:151-7. 2016
    ..Selective inhibitors of the cholesterol esterifying enzyme sterol-O acyltransferase 2 (SOAT2) hold great promise as effective CVD therapeutics...
  20. pmc Potentiating the antitumour response of CD8(+) T cells by modulating cholesterol metabolism
    Wei Yang
    State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Science Research Center, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Nature 531:651-5. 2016
    ..ACAT1, an established target for atherosclerosis, is therefore also a potential target for cancer immunotherapy. ..
  21. pmc Cellular pregnenolone esterification by acyl-CoA:cholesterol acyltransferase
    Maximillian A Rogers
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 287:17483-92. 2012
    ..Acyl-CoA:cholesterol acyltransferase 1 and 2 (ACAT1 and ACAT2) convert various sterols to steryl esters; their activities are activated by cholesterol...
  22. pmc ACAT2 and ABCG5/G8 are both required for efficient cholesterol absorption in mice: evidence from thoracic lymph duct cannulation
    Tam M Nguyen
    Department of Pathology, Wake Forest School of Medicine, Winston Salem, NC 27157, USA
    J Lipid Res 53:1598-609. 2012
    ..through adenosine triphosphate-binding cassette transporter G5 and G8 heterodimer (G5G8), and acyl CoA:cholesterol acyltransferase 2 (ACAT2)...
  23. pmc Ezetimibe blocks the internalization of NPC1L1 and cholesterol in mouse small intestine
    Chang Xie
    The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    J Lipid Res 53:2092-101. 2012
    ..Acyl-CoA cholesterol acyltransferase 2 (ACAT2), another important protein for cholesterol absorption by providing cholesteryl esters to ..
  24. pmc Multiple mechanisms limit the accumulation of unesterified cholesterol in the small intestine of mice deficient in both ACAT2 and ABCA1
    Stephen D Turley
    Dept of Internal Medicine, Univ of Texas Southwestern Medical School, 5323 Harry Hines Blvd, Dallas, TX 75390 9151, USA
    Am J Physiol Gastrointest Liver Physiol 299:G1012-22. 2010
    ..The effect of deleting these genes, particularly acyl CoA:cholesterol acyl transferase 2 (ACAT2), on cholesterol absorption and fecal sterol excretion is well documented...
  25. ncbi The gene encoding PRBP, the mouse homolog of human TRBP, maps to distal chromosome 15
    J Zhong
    Department of Genetics, Box 357360, University of Washington, Seattle, Washington 98195, USA
    Mamm Genome 9:413-4. 1998
  26. pmc ACAT1 gene ablation increases 24(S)-hydroxycholesterol content in the brain and ameliorates amyloid pathology in mice with AD
    Elena Y Bryleva
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 107:3081-6. 2010
    ..Acyl-CoA:cholesterol acyltransferases (ACAT1 and ACAT2) are two enzymes that convert free cholesterol to cholesteryl esters...
  27. pmc Identification of a gene encoding MGAT1, a monoacylglycerol acyltransferase
    Chi Liang Eric Yen
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141 1900, USA
    Proc Natl Acad Sci U S A 99:8512-7. 2002
    ..The identification of the MGAT1 gene should greatly facilitate research on the identification of the intestinal MGAT gene and on the function of MGAT enzymes in mammalian glycerolipid metabolism...
  28. ncbi ACAT-2, a second mammalian acyl-CoA:cholesterol acyltransferase. Its cloning, expression, and characterization
    S Cases
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, California 94141, USA
    J Biol Chem 273:26755-64. 1998
    ..The identification and cloning of ACAT-2 will facilitate molecular approaches to understanding the role of ACAT enzymes in mammalian biology...

Research Grants1