Rap1b

Summary

Gene Symbol: Rap1b
Description: RAS related protein 1b
Alias: 2810443E11Rik, ras-related protein Rap-1b, GTP-binding protein smg p21B
Species: mouse

Top Publications

  1. pmc Rap1 promotes VEGFR2 activation and angiogenesis by a mechanism involving integrin αvβ₃
    Sribalaji Lakshmikanthan
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    Blood 118:2015-26. 2011
  2. pmc Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes
    Aradhana Awasthi
    Molecular Immunology, Blood Research Institute, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Exp Med 207:1923-38. 2010
  3. pmc Integrin-independent role of CalDAG-GEFI in neutrophil chemotaxis
    Carla Carbo
    Immune Disease Institute, Program in Cellular and Molecular Medicine, Department of Laboratory Medicine, Children s Hospital, and Department of Pathology, Harvard Medical School, 3 Blackfan Circle, Boston, MA 02115, USA
    J Leukoc Biol 88:313-9. 2010
  4. pmc Rap1b is required for normal platelet function and hemostasis in mice
    Magdalena Chrzanowska-Wodnicka
    Department of Medicine and Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Clin Invest 115:680-7. 2005
  5. doi Role of the small GTPase Rap1 for integrin activity regulation in endothelial cells and angiogenesis
    Guillaume Carmona
    Molecular Cardiology, Department of Internal Medicine III, J W Goethe University Frankfurt, Frankfurt
    Blood 113:488-97. 2009
  6. pmc Defective angiogenesis, endothelial migration, proliferation, and MAPK signaling in Rap1b-deficient mice
    Magdalena Chrzanowska-Wodnicka
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    Blood 111:2647-56. 2008
  7. pmc Rap1a null mice have altered myeloid cell functions suggesting distinct roles for the closely related Rap1a and 1b proteins
    Yu Li
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202, USA
    J Immunol 179:8322-31. 2007
  8. doi The rap GTPases regulate B cell morphology, immune-synapse formation, and signaling by particulate B cell receptor ligands
    Kevin B L Lin
    Department of Microbiology and Immunology, I3 and CELL Research Groups, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Immunity 28:75-87. 2008
  9. pmc Rap1b regulates B cell development, homing, and T cell-dependent humoral immunity
    Haiyan Chu
    Laboratory of Molecular Immunology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 181:3373-83. 2008
  10. pmc Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis
    Rupalatha Maddala
    Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA
    Dev Biol 406:74-91. 2015

Scientific Experts

  • Jaichandar Subramanian
  • Magdalena Chrzanowska-Wodnicka
  • Maria Eugenia Sabbatini
  • Sribalaji Lakshmikanthan
  • Gilbert C White
  • Alexei Morozov
  • Rupalatha Maddala
  • Lawrence A Quilliam
  • Aradhana Awasthi
  • Haiyan Chu
  • Kentaro Kaneko
  • Michael E Cahill
  • Sayaka Ishihara
  • Wenjuan Su
  • Marjorie Côte
  • Xiaodong Zheng
  • Aniko Szabo
  • David X Zhang
  • Sachin Kumar
  • Erika S Wittchen
  • Wei Zou
  • Ponugoti V Rao
  • Richard A Lang
  • Magdalena Sobczak
  • Guoying Zhang
  • Reuben Kapur
  • Carla Carbo
  • Subramaniam Malarkannan
  • Guillaume Carmona
  • Z Wang
  • Yuhong Chen
  • Bing Xing Pan
  • Kevin B L Lin
  • Lin Sun
  • Motonori Takahashi
  • Yu Li
  • Bruno Bernardi
  • Fernando Ribeiro-Neto
  • Jacques T Weissman
  • Haosheng Sun
  • Siyu S Chen
  • Amy M Gancarz
  • Hannah M Cates
  • Amy Ng
  • Zi jun Wang
  • Jian Feng
  • Pingwen Xu
  • Rosemary C Bagot
  • Ja Wook Koo
  • Makoto Fukuda
  • Elizabeth A Heller
  • Deena M Walker
  • David M Dietz
  • Elizabeth L Cordonier
  • Pamela J Kennedy
  • Yong Xu
  • Eric J Nestler
  • Li Shen
  • Rachael L Neve
  • Makoto Saegusa
  • Mark R Philips
  • Koko Katagiri
  • Wolfgang Bergmeier
  • Joseph Wynne
  • Frank B Gertler
  • Klaus Ley
  • Stéphane Bécart
  • Emilie Montenont
  • Masayuki Miyasaka
  • Adam Mor
  • Elaine M Pinheiro
  • Marianne Strazza
  • Tharkika Nagendran
  • Camille Fos
  • Jeannette Vasquez-Vivar
  • David S Paul
  • Yoshinori Nishijima
  • Jeffrey Berger
  • Amnon Altman
  • Akihiko Nishikimi
  • Eiji Umemoto
  • Ann J Canonigo-Balancio
  • Ko Momotani
  • John A Auchampach
  • Bartosz J Zieba
  • Juying Xu
  • Rupali Sani Kumar
  • Jason E Maas
  • Matthew Kofron
  • David L Mattson

Detail Information

Publications37

  1. pmc Rap1 promotes VEGFR2 activation and angiogenesis by a mechanism involving integrin αvβ₃
    Sribalaji Lakshmikanthan
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    Blood 118:2015-26. 2011
    ..Rap1a and Rap1b, 2 highly homologous small G proteins, are both required for angiogenesis in vivo and for normal EC responses to ..
  2. pmc Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes
    Aradhana Awasthi
    Molecular Immunology, Blood Research Institute, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Exp Med 207:1923-38. 2010
    ..Using Rap1a or Rap1b knockout mice, we identify Rap1b as the major isoform in NK cells...
  3. pmc Integrin-independent role of CalDAG-GEFI in neutrophil chemotaxis
    Carla Carbo
    Immune Disease Institute, Program in Cellular and Molecular Medicine, Department of Laboratory Medicine, Children s Hospital, and Department of Pathology, Harvard Medical School, 3 Blackfan Circle, Boston, MA 02115, USA
    J Leukoc Biol 88:313-9. 2010
    ..Here, we explored the role of CalDAG-GEFI and Rap1b in integrin-independent neutrophil chemotaxis...
  4. pmc Rap1b is required for normal platelet function and hemostasis in mice
    Magdalena Chrzanowska-Wodnicka
    Department of Medicine and Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Clin Invest 115:680-7. 2005
    b>Rap1b, an abundant small GTPase in platelets, becomes rapidly activated upon stimulation with agonists...
  5. doi Role of the small GTPase Rap1 for integrin activity regulation in endothelial cells and angiogenesis
    Guillaume Carmona
    Molecular Cardiology, Department of Internal Medicine III, J W Goethe University Frankfurt, Frankfurt
    Blood 113:488-97. 2009
    ..Human umbilical vein endothelial cells (HUVECs) express Rap1a and Rap1b mRNA...
  6. pmc Defective angiogenesis, endothelial migration, proliferation, and MAPK signaling in Rap1b-deficient mice
    Magdalena Chrzanowska-Wodnicka
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    Blood 111:2647-56. 2008
    ..Here we identify a small GTPase, Rap1b, as a positive regulator of angiogenesis...
  7. pmc Rap1a null mice have altered myeloid cell functions suggesting distinct roles for the closely related Rap1a and 1b proteins
    Yu Li
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202, USA
    J Immunol 179:8322-31. 2007
    ....
  8. doi The rap GTPases regulate B cell morphology, immune-synapse formation, and signaling by particulate B cell receptor ligands
    Kevin B L Lin
    Department of Microbiology and Immunology, I3 and CELL Research Groups, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Immunity 28:75-87. 2008
    ..Thus by controlling B cell morphology and cytoskeletal organization, Rap might play a key role in the activation of B cells by particulate and cell-associated antigens...
  9. pmc Rap1b regulates B cell development, homing, and T cell-dependent humoral immunity
    Haiyan Chu
    Laboratory of Molecular Immunology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 181:3373-83. 2008
    ..Rap1 exists in two isoforms: Rap1a and Rap1b. Although Rap1 has been extensively studied, its isoform-specific functions in B cells have not been elucidated...
  10. pmc Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis
    Rupalatha Maddala
    Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA
    Dev Biol 406:74-91. 2015
    ..While individual germline deficiency of either Rap1a or Rap1b did not cause overt defects in mouse lens, conditional double deficiency (Rap1 cKO) prior to lens placode ..
  11. pmc Rap1 GTPase activation and barrier enhancement in rpe inhibits choroidal neovascularization in vivo
    Erika S Wittchen
    Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, North Carolina, United States of America
    PLoS ONE 8:e73070. 2013
    ..b>Rap1B isoforms is less clear...
  12. pmc Rap1b in smooth muscle and endothelium is required for maintenance of vascular tone and normal blood pressure
    Sribalaji Lakshmikanthan
    From the Blood Research Institute, BloodCenter of Wisconsin, Milwaukee S L, M C W Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville B J Z, K M, M V A, A V S and Department of Pharmacology and Toxicology Z D G, J A A, Cardiovascular Center Z D G, X Z, J E M, D X Z, J A A, Department of Medicine X Z, J E M, D X Z, Department of Physiology H L, D L M, and Division of Biostatistics A S, Medical College of Wisconsin, Milwaukee
    Arterioscler Thromb Vasc Biol 34:1486-94. 2014
    Small GTPase Ras-related protein 1 (Rap1b) controls several basic cellular phenomena, and its deletion in mice leads to several cardiovascular defects, including impaired adhesion of blood cells and defective angiogenesis...
  13. pmc The small GTPase Rap1b negatively regulates neutrophil chemotaxis and transcellular diapedesis by inhibiting Akt activation
    Sachin Kumar
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation, Cincinnati, OH 45229 University of Cincinnati College of Medicine, Cincinnati OH 45229
    J Exp Med 211:1741-58. 2014
    ..The small GTPase Rap1b is generally viewed as a positive regulator of immune cell functions by controlling bidirectional integrin ..
  14. pmc Rap1 promotes endothelial mechanosensing complex formation, NO release and normal endothelial function
    Sribalaji Lakshmikanthan
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, USA
    EMBO Rep 16:628-37. 2015
    ..Our study establishes a novel paradigm for Rap1 as a regulator of mechanotransduction. ..
  15. pmc Neuronal Rap1 Regulates Energy Balance, Glucose Homeostasis, and Leptin Actions
    Kentaro Kaneko
    Children s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell Rep 16:3003-15. 2016
    ..Thus, neuronal Rap1 critically regulates leptin sensitivity and mediates HFD-induced obesity and hypothalamic pathology and may represent a potential therapeutic target for obesity treatment. ..
  16. pmc Rap1 and its effector RIAM are required for lymphocyte trafficking
    Wenjuan Su
    Perlmutter Cancer Institute and
    Blood 126:2695-703. 2015
    ..Using mice conditionally deficient in both Rap1a and Rap1b and mice null for RIAM, we show that the Rap1/RIAM module is not required for T- or B-cell development but is ..
  17. pmc SLAT promotes TCR-mediated, Rap1-dependent LFA-1 activation and adhesion through interaction of its PH domain with Rap1
    Marjorie Côte
    Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    J Cell Sci 128:4341-52. 2015
    ..of inside-out signaling, namely the active form of the small GTPase Rap1 (which has two isoforms, Rap1A and Rap1B)...
  18. pmc Dual functions of Rap1 are crucial for T-cell homeostasis and prevention of spontaneous colitis
    Sayaka Ishihara
    Department of Biosciences, School of Science, Kitasato University, 1 15 1 Kitasato, Minamiku, Sagamihara, Kanagawa 252 0373, Japan
    Nat Commun 6:8982. 2015
    ..This Rap1-dependent regulation of T-cell circulation prevents the onset of colitis. ..
  19. pmc Small GTPase Rap1 Is Essential for Mouse Development and Formation of Functional Vasculature
    Magdalena Chrzanowska-Wodnicka
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, 53201, United States of America
    PLoS ONE 10:e0145689. 2015
    ..In vitro, several Rap1 effectors and regulatory mechanisms have been proposed. In particular, Rap1 has been implicated in maintaining epithelial and endothelial cell junction integrity and linked with cerebral cavernous malformations...
  20. pmc Bidirectional Synaptic Structural Plasticity after Chronic Cocaine Administration Occurs through Rap1 Small GTPase Signaling
    Michael E Cahill
    Fishberg Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
    Neuron 89:566-82. 2016
    ..Our findings reveal key roles for the bidirectional synaptic expression of the Rap1b small GTPase and an associated local synaptic protein translation network in this process...
  21. pmc Rac1 GTPase-deficient mouse lens exhibits defects in shape, suture formation, fiber cell migration and survival
    Rupalatha Maddala
    Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA
    Dev Biol 360:30-43. 2011
    ....
  22. pmc Talin1 and Rap1 are critical for osteoclast function
    Wei Zou
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri, USA
    Mol Cell Biol 33:830-44. 2013
    ..Thus, talin1 and Rap1 are critical for resorptive function, and their selective inhibition in mature osteoclasts retards pathological bone loss...
  23. pmc Rap1 signaling prevents L-type calcium channel-dependent neurotransmitter release
    Jaichandar Subramanian
    Unit on Behavioral Genetics, Laboratory of Molecular Pathophysiology, National Institute of Mental Health and Microscopy and Imaging Core, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Neurosci 33:7245-52. 2013
    ..We propose that the Rap1 signaling enables plasticity and fear learning by regulating LTCCs at cortico-amygdala synapses...
  24. pmc Distinct roles for Rap1b protein in platelet secretion and integrin αIIbβ3 outside-in signaling
    Guoying Zhang
    Division of Cardiovascular Medicine, The Gill Heart Institute, University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 286:39466-77. 2011
    b>Rap1b is activated by platelet agonists and plays a critical role in integrin α(IIb)β(3) inside-out signaling and platelet aggregation...
  25. pmc Rap1b is critical for glycoprotein VI-mediated but not ADP receptor-mediated alpha2beta1 activation
    Z Wang
    Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7260, USA
    J Thromb Haemost 7:693-700. 2009
    ..However, signaling molecules that regulate alpha2beta1 activation in platelets are not well defined. A strong candidate molecule is the small GTPase Rap1b, the dominant platelet isoform of Rap1, which regulates alphaIIbbeta3 activation.
  26. pmc Rap1b GTPase ameliorates glucose-induced mitochondrial dysfunction
    Lin Sun
    Department of Pathology, Northwestern University, Chicago, Illinois 60611, USA
    J Am Soc Nephrol 19:2293-301. 2008
    ..The GTPase Ras-proximate-1 (Rap1b) is upregulated in the hyperglycemic state and is known to increase B-Raf, an antiapoptotic effector protein...
  27. pmc Rap1 activation plays a regulatory role in pancreatic amylase secretion
    Maria E Sabbatini
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109 0622, USA
    J Biol Chem 283:23884-94. 2008
    ..Rap1 activation not only mediates the cAMP-evoked response via Epac1 but is also involved in CCK- and carbachol-induced amylase release, with their action most likely mediated by CalDAG-GEF III...
  28. doi Sequential activation of Rap1 and Rac1 small G proteins by PDGF locally at leading edges of NIH3T3 cells
    Motonori Takahashi
    Department of Biochemistry and Molecular Biology, Division of Molecular and Cellular Biology, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Genes Cells 13:549-69. 2008
    ..This process is pivotal for the PDGF-induced formation of leading edge structures and cell movement...
  29. pmc A critical role of Rap1b in B-cell trafficking and marginal zone B-cell development
    Yuhong Chen
    Blood Research Institute, Blood Center of Wisconsin, Milwaukee, WI 53226, USA
    Blood 111:4627-36. 2008
    ..Rap1 is a member of the Ras superfamily of small GTP-binding proteins and has 2 isoforms, Rap1a and Rap1b. Although Rap1 has been suggested to have an important role in a variety of cellular processes, no direct evidence ..
  30. doi Enhanced cortico-amygdala efficacy and suppressed fear in absence of Rap1
    Bing Xing Pan
    Unit on Behavioral Genetics, Laboratory of Molecular Pathophysiology, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Neurosci 28:2089-98. 2008
    ..Here, we report that a CaMKII-alpha-Cre-mediated knock-out (KO) of the rap1a and rap1b genes impaired synaptic plasticity and increased basal synaptic transmission in the cortical but not thalamic ..
  31. pmc The small GTPase Rap1b regulates the cross talk between platelet integrin alpha2beta1 and integrin alphaIIbbeta3
    Bruno Bernardi
    Department of Biochemistry, University of Pavia, via Bassi 21, 27100 Pavia, Italy
    Blood 107:2728-35. 2006
    The involvement of the small GTPase Rap1b in platelet integrin alpha2beta1-dependent outside-in signaling was investigated...
  32. ncbi cAMP-dependent oncogenic action of Rap1b in the thyroid gland
    Fernando Ribeiro-Neto
    Department of Pharmacology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:46868-75. 2004
    cAMP signaling leads to activation and phosphorylation of Rap1b. Using cellular models where cAMP stimulates cell proliferation, we have demonstrated that cAMP-mediated activation, as well as phosphorylation of Rap1b, is critical for ..
  33. ncbi G-protein-coupled receptor-mediated activation of rap GTPases: characterization of a novel Galphai regulated pathway
    Jacques T Weissman
    ACADIA Pharmaceuticals Inc, 3911 Sorrento Valley Blvd, San Diego, CA 92121, USA
    Oncogene 23:241-9. 2004
    ..A constitutively active mutant of Galphai2 also mediated Ras/rap-dependent responses. These data indicate that GPCRs coupled to pertussis-toxin-sensitive G-proteins activate Rap through a Galpha subunit, C3G, and Src-dependent pathway...
  34. ncbi cAMP-GEFII is a direct target of cAMP in regulated exocytosis
    N Ozaki
    Department of Molecular Medicine, Chiba University Graduate School of Medicine, Chiba 260 8670, Japan
    Nat Cell Biol 2:805-11. 2000
    ..Accordingly, cAMP-GEFII is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-independent exocytosis...
  35. ncbi Expression of Rap 1 suppresses genomic instability of H-ras transformed mouse fibroblasts
    M A Wani
    Department of Cell Biology, University of Cincinnati, College of Medicine, Ohio 45267 0521, USA
    Somat Cell Mol Genet 23:123-33. 1997
    ..Furthermore fluorescent-in-situ hybridization (FISH) with a pancentromeric mouse probe showed that elevated levels of chromosome aberrations in cells expressing H-ras were also suppressed after co-expression of Rap 1...
  36. ncbi The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach
    P Chavrier
    Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, F R G
    Gene 112:261-4. 1992
    ..These results confirm that the overall diversity of the Rab and Rho protein subfamilies account for more than thirty different members in mammalian cells...