Rab27a

Summary

Gene Symbol: Rab27a
Description: RAB27A, member RAS oncogene family
Alias: 2210402C08Rik, 2410003M20Rik, 4933437C11Rik, ash, ras-related protein Rab-27A, ashen
Species: mouse

Top Publications

  1. ncbi Rab27a regulates phagosomal pH and NADPH oxidase recruitment to dendritic cell phagosomes
    Carolina Jancic
    Institut Curie, INSERM U653, Immunité et Cancer, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Nat Cell Biol 9:367-78. 2007
  2. doi A Griscelli syndrome type 2 murine model of hemophagocytic lymphohistiocytosis (HLH)
    Jana Pachlopnik Schmid
    Institut National de la Santé et de la Recherche Médicale U768, Laboratoire du Développement Normal et Pathologique du Système Immunitaire, Paris, France
    Eur J Immunol 38:3219-25. 2008
  3. doi Actin assembly controlled by GDP-Rab27a is essential for endocytosis of the insulin secretory membrane
    Toshihide Kimura
    Department of Pharmacology, Oita University Faculty of Medicine at Oita, Japan
    Arch Biochem Biophys 496:33-7. 2010
  4. doi The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines
    Toshihide Kimura
    Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita 879 5593, Japan
    J Cell Sci 121:3092-8. 2008
  5. pmc Rab27b is expressed in a wide range of exocytic cells and involved in the delivery of secretory granules near the plasma membrane
    Hiroshi Gomi
    Laboratory of Molecular Endocrinology and Metabolism, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
    Mol Biol Cell 18:4377-86. 2007
  6. pmc Rab27a and Rab27b regulate neutrophil azurophilic granule exocytosis and NADPH oxidase activity by independent mechanisms
    Jennifer L Johnson
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Traffic 11:533-47. 2010
  7. doi Elucidation of Rab27 recruitment by its effectors: structure of Rab27a bound to Exophilin4/Slp2-a
    Leonard M G Chavas
    Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
    Structure 16:1468-77. 2008
  8. pmc A general role for Rab27a in secretory cells
    Tanya Tolmachova
    Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, London SW7 2AZ, United Kingdom
    Mol Biol Cell 15:332-44. 2004
  9. pmc Role of myosin VIIa and Rab27a in the motility and localization of RPE melanosomes
    Daniel Gibbs
    Department of Pharmacology, UCSD School of Medicine, La Jolla, CA 92093 0912, USA
    J Cell Sci 117:6473-83. 2004
  10. ncbi Synaptotagmin-like protein (Slp) homology domain 1 of Slac2-a/melanophilin is a critical determinant of GTP-dependent specific binding to Rab27A
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:40118-24. 2002

Research Grants

  1. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002

Scientific Experts

  • Leonard M G Chavas
  • Paige Lacy
  • Mitsunori Fukuda
  • Abraham L Kierszenbaum
  • J H Nadeau
  • Alistair N Hume
  • Jana Pachlopnik Schmid
  • Alberto Fraile-Ramos
  • Miguel C Seabra
  • Tanya Tolmachova
  • Toshihide Kimura
  • Jennifer L Johnson
  • Jose S Ramalho
  • Sergio D Catz
  • Tetsuro Izumi
  • Christina Wasmeier
  • Takao Yasuda
  • Rajesh K Singh
  • Jlenia Monfregola
  • Ichiro Niki
  • Taruho S Kuroda
  • Abul K Tarafder
  • Edward K Novak
  • Silene T Wavre-Shapton
  • Clare E Futter
  • Chiara Recchi
  • Hong Hong
  • Richard T Swank
  • Ana C Figueiredo
  • Daniela B Munafo
  • Cui Chang
  • Yanan Hou
  • Mami Yamaoka
  • Megumi Shimada-Sugawara
  • Nadine Reichhart
  • Hiroshi Gomi
  • Mafalda Lopes da Silva
  • Birthe Jessen
  • Fernando E Sepulveda
  • Genevieve De Saint Basile
  • Angélique Bobrie
  • T D Andrews
  • Gang Chen
  • Lilian Chiang
  • Giulia Bolasco
  • Yoshiaki Kariya
  • Yoshinobu Koguchi
  • Dongfang Liu
  • Chika Saegusa
  • Karen E Anderson
  • Amanda L Blasius
  • Shigeki Taniguchi
  • William A Gahl
  • Wei Li
  • Anat Benado
  • Ross Anders
  • Duarte C Barral
  • Bruce Beutler
  • Sophie Rutschmann
  • Beverly A Ellis
  • Christine Chiaverini
  • Oliver Holt
  • Matthew J Merrins
  • Seong Hyun Kim
  • Hironori Higashio
  • Wai Tsing Chan
  • Sreenivasulu Chintala
  • Carolina Jancic
  • X Wu
  • Y Zhang
  • Giovanna Bossi
  • Takashi Itoh
  • Gillian M Griffiths
  • Kazuo Kasai
  • Marion L Chabrillat
  • Kalwant S Authi
  • Rashi Gautam
  • Thuyen Nguyen
  • Daniel Gibbs
  • Shengli Zhao
  • Masahiro Takei
  • Stephen A Ernst
  • Kohichi Matsunaga
  • Toshimasa Ishizaki
  • Takeshi Terabayashi
  • Sanjay Tiwari
  • Mathias W Seeliger
  • John A Williams
  • Wenxing Qin
  • Tomoki Nishioka

Detail Information

Publications81

  1. ncbi Rab27a regulates phagosomal pH and NADPH oxidase recruitment to dendritic cell phagosomes
    Carolina Jancic
    Institut Curie, INSERM U653, Immunité et Cancer, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Nat Cell Biol 9:367-78. 2007
    ..Here, we show that dendritic cells derived from Rab27a-deficient ashen mice show increased phagosome acidification and antigen degradation, causing a defect in antigen ..
  2. doi A Griscelli syndrome type 2 murine model of hemophagocytic lymphohistiocytosis (HLH)
    Jana Pachlopnik Schmid
    Institut National de la Santé et de la Recherche Médicale U768, Laboratoire du Développement Normal et Pathologique du Système Immunitaire, Paris, France
    Eur J Immunol 38:3219-25. 2008
    Griscelli syndrome type 2 is caused by mutations in the RAB27A gene and is a rare and potentially fatal immune disorder associated with hemophagocytic lymphohistiocytosis (HLH)...
  3. doi Actin assembly controlled by GDP-Rab27a is essential for endocytosis of the insulin secretory membrane
    Toshihide Kimura
    Department of Pharmacology, Oita University Faculty of Medicine at Oita, Japan
    Arch Biochem Biophys 496:33-7. 2010
    We have recently reported that GDP-bound Rab27a regulates endocytosis of the insulin secretory membrane via its binding to coronin 3, an actin-binding protein...
  4. doi The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines
    Toshihide Kimura
    Department of Pharmacology, Oita University Faculty of Medicine, Hasama, Yufu, Oita 879 5593, Japan
    J Cell Sci 121:3092-8. 2008
    b>Rab27a is involved in the control of membrane traffic, a crucial step in the regulated secretion...
  5. pmc Rab27b is expressed in a wide range of exocytic cells and involved in the delivery of secretory granules near the plasma membrane
    Hiroshi Gomi
    Laboratory of Molecular Endocrinology and Metabolism, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
    Mol Biol Cell 18:4377-86. 2007
    Rab proteins regulate multiple, complex processes of membrane traffic. Among these proteins, Rab27a has been shown to function specifically in regulated exocytic pathways...
  6. pmc Rab27a and Rab27b regulate neutrophil azurophilic granule exocytosis and NADPH oxidase activity by independent mechanisms
    Jennifer L Johnson
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Traffic 11:533-47. 2010
    ..In vivo studies showed that the small GTPase Rab27a regulates azurophilic granule exocytosis...
  7. doi Elucidation of Rab27 recruitment by its effectors: structure of Rab27a bound to Exophilin4/Slp2-a
    Leonard M G Chavas
    Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
    Structure 16:1468-77. 2008
    ..b>Rab27a regulates numerous exocytotic pathways, and its dysfunction causes the Griscelli syndrome human immunodeficiency...
  8. pmc A general role for Rab27a in secretory cells
    Tanya Tolmachova
    Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, London SW7 2AZ, United Kingdom
    Mol Biol Cell 15:332-44. 2004
    ..We constructed a PAC-based transgenic mouse, which expresses EGFP-Rab27a under the control of endogenous Rab27a promoter...
  9. pmc Role of myosin VIIa and Rab27a in the motility and localization of RPE melanosomes
    Daniel Gibbs
    Department of Pharmacology, UCSD School of Medicine, La Jolla, CA 92093 0912, USA
    J Cell Sci 117:6473-83. 2004
    ..in RPE cells that could potentially participate in melanosome transport, but of those tested, only myosin VIIa and Rab27a were found to be required for normal distribution...
  10. ncbi Synaptotagmin-like protein (Slp) homology domain 1 of Slac2-a/melanophilin is a critical determinant of GTP-dependent specific binding to Rab27A
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:40118-24. 2002
    ..protein (Slp) homology domain (SHD) of the Slp and Slac2 families has recently been identified as a specific Rab27A-binding domain (Kuroda, T. S., Fukuda, M., Ariga, H., and Mikoshiba, K. (2002) J. Biol. Chem...
  11. pmc Functional redundancy of Rab27 proteins and the pathogenesis of Griscelli syndrome
    Duarte C Barral
    Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London, United Kingdom
    J Clin Invest 110:247-57. 2002
    ..This disease is caused by loss-of-function mutations in RAB27A, which encodes 1 of the 60 known Rab GTPases, critical regulators of vesicular transport...
  12. pmc Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation
    Kazuo Kasai
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    J Clin Invest 115:388-96. 2005
    The monomeric small GTPase Rab27a is specifically localized on both secretory granules and lysosome-related organelles...
  13. pmc Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes
    Ana C Figueiredo
    Molecular Medicine, National Heart and Lung Institute, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 283:23209-16. 2008
    ..b>Rab27a controls motility and regulated exocytosis of secretory granules and related organelles...
  14. ncbi A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport
    Molly Strom
    Department of Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London SW7 2AZ, United Kingdom
    J Biol Chem 277:25423-30. 2002
    The Rab27a GTPase regulates diverse processes involving lysosome-related organelles, including melanosome motility in melanocytes, and lytic granule release in cytotoxic T lymphocytes...
  15. ncbi The leaden gene product is required with Rab27a to recruit myosin Va to melanosomes in melanocytes
    Alistair N Hume
    Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London SW7 2AZ, UK
    Traffic 3:193-202. 2002
    ..Griscelli syndrome results from loss of function mutations in either the RAB27A (type 1 Griscelli syndrome) or MYO5A (type 2 Griscelli syndrome) genes...
  16. pmc The Rab27a/granuphilin complex regulates the exocytosis of insulin-containing dense-core granules
    Zhaohong Yi
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi, Gunma 371 8512, Japan
    Mol Cell Biol 22:1858-67. 2002
    ..by a yeast two-hybrid system revealed that an amino-terminal zinc-finger domain of granuphilin interacts with Rab27a. Granuphilin preferentially bound to the GTP form of Rab27a...
  17. pmc Defective granule exocytosis in Rab27a-deficient lymphocytes from Ashen mice
    E K Haddad
    Experimental Immunology Branch, National Cancer Institute, and
    J Cell Biol 152:835-42. 2001
    Because mutations in Rab27a have been linked to immune defects in humans, we have examined cytotoxic lymphocyte function in ashen mice, which contain a splicing mutation in Rab27a...
  18. pmc Rab27a is required for regulated secretion in cytotoxic T lymphocytes
    J C Stinchcombe
    Sir William Dunn School of Pathology, University of Oxford, OX1 3RE, United Kingdom
    J Cell Biol 152:825-34. 2001
    b>Rab27a activity is affected in several mouse models of human disease including Griscelli (ashen mice) and Hermansky-Pudlak (gunmetal mice) syndromes...
  19. pmc Rab27a regulates the peripheral distribution of melanosomes in melanocytes
    A N Hume
    Cell and Molecular Biology Section, Division of Biomedical Sciences, London, United Kingdom
    J Cell Biol 152:795-808. 2001
    Rab GTPases are regulators of intracellular membrane traffic. We report a possible function of Rab27a, a protein implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, ..
  20. ncbi Rab27a enables myosin Va-dependent melanosome capture by recruiting the myosin to the organelle
    X Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Sci 114:1091-100. 2001
    ..Genetic evidence suggests that Rab27a, the product of the ashen locus, functions with myosin Va in this process...
  21. pmc A mutation in Rab27a causes the vesicle transport defects observed in ashen mice
    S M Wilson
    Mouse Cancer Genetics Program, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 97:7933-8. 2000
    ..Here, using positional cloning and bacterial artificial chromosome rescue, we show that ash encodes Rab27a. Rab GTPases represent the largest branch of the p21 Ras superfamily and are recognized as key players in ..
  22. pmc Rab27b regulates number and secretion of platelet dense granules
    Tanya Tolmachova
    Molecular and Cellular Medicine, National Heart and Lung Institute, Imperial College London, London SW7 2AZ, United Kingdom
    Proc Natl Acad Sci U S A 104:5872-7. 2007
    The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Rab27a has been shown previously to regulate organelle movement and regulated exocytosis in a wide variety of secretory cells...
  23. pmc The murine dilute suppressor gene dsu suppresses the coat-color phenotype of three pigment mutations that alter melanocyte morphology, d, ash and ln
    K J Moore
    Mammalian Genetics Laboratory, Bionetics Research, Inc, Frederick, Maryland
    Genetics 119:933-41. 1988
    ..In addition to d, two other loci, ashen (ash) and leaden (ln), have been identified that produce a diluted coat color associated with adendritic ..
  24. ncbi Rab27a and MyoVa are the primary Mlph interactors regulating melanosome transport in melanocytes
    Alistair N Hume
    Molecular and Cellular Medicine, National Heart and Lung Institute, Imperial College London, London, SW7 2AZ, UK
    J Cell Sci 120:3111-22. 2007
    ..Melanophilin (Mlph) is a Rab27a- and myosin Va (MyoVa)-binding protein that regulates this process...
  25. pmc Slp1 and Slp2-a localize to the plasma membrane of CTL and contribute to secretion from the immunological synapse
    Oliver Holt
    Sir William Dunn School of Pathology, University of Oxford, South Parks Rd, Oxford, OX1 3RE, UK
    Traffic 9:446-57. 2008
    b>Rab27a is required for polarized secretion of lysosomes from cytotoxic T lymphocytes (CTLs) at the immunological synapse...
  26. pmc Rab27a is a key component of the secretory machinery of azurophilic granules in granulocytes
    Daniela B Munafo
    Department of Molecular and Experimental Medicine, Division of Biochemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Biochem J 402:229-39. 2007
    ..The secretory machinery utilized by neutrophils is poorly characterized. We show that the small GTPase Rab27a is an essential component of the secretory machinery of azurophilic granules in granulocytes...
  27. ncbi Rab27A-binding protein Slp2-a is required for peripheral melanosome distribution and elongated cell shape in melanocytes
    Taruho S Kuroda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Nat Cell Biol 6:1195-203. 2004
    The synaptotagmin-like protein (Slp) family is implicated in regulating Rab27A-mediated membrane transport, but how it might do this is unknown...
  28. pmc The role of Rab27a in the regulation of melanosome distribution within retinal pigment epithelial cells
    Clare E Futter
    Division of Cell Biology, Institute of Ophthalmology, University College London, London EC1V 9EL, United Kingdom
    Mol Biol Cell 15:2264-75. 2004
    ..distribution is due to the interaction with the cortical actin cytoskeleton mediated by a tripartite complex of Rab27a, melanophilin, and myosin Va...
  29. ncbi Identification of an organelle receptor for myosin-Va
    Xufeng S Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Cell Biol 4:271-8. 2002
    ..motor, binds to one of its cargoes, the melanosome, by interacting with a receptor-protein complex containing Rab27a and melanophilin, a postulated Rab27a effector...
  30. pmc Slc15a4, AP-3, and Hermansky-Pudlak syndrome proteins are required for Toll-like receptor signaling in plasmacytoid dendritic cells
    Amanda L Blasius
    Department of Genetics, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 107:19973-8. 2010
    ..These proteins are not necessary for TLR7 or TLR9 signaling in conventional DCs and thus comprise a membrane trafficking pathway uniquely required for endosomal TLR signaling in pDCs...
  31. pmc Loss of Rab27 function results in abnormal lung epithelium structure in mice
    Giulia Bolasco
    Molecular Medicine, National Heart and Lung Institute, Imperial College London, London, United Kingdom
    Am J Physiol Cell Physiol 300:C466-76. 2011
    ..Previous studies indicated that Rab27a and Rab27b are expressed in the murine lung suggesting that they regulate secretory processes in the lung...
  32. pmc Massively parallel sequencing of the mouse exome to accurately identify rare, induced mutations: an immediate source for thousands of new mouse models
    T D Andrews
    Immunogenomics Laboratory, Australian National University, GPO Box 334, Canberra City, Australian Capital Territory, 2601, Australia
    Open Biol 2:120061. 2012
    ..This approach transforms genetic screens in mice, establishes a general strategy for analysing rare DNA variants and opens up a large new source for experimental models of human disease...
  33. pmc Rab27 effector Slp2-a transports the apical signaling molecule podocalyxin to the apical surface of MDCK II cells and regulates claudin-2 expression
    Takao Yasuda
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aoba ku, Sendai, Miyagi, Japan
    Mol Biol Cell 23:3229-39. 2012
    ..reveal that Slp2-a is required for targeting of the signaling molecule podocalyxin to the apical membrane in a Rab27A-dependent manner...
  34. pmc MUNC13-4 protein regulates the oxidative response and is essential for phagosomal maturation and bacterial killing in neutrophils
    Jlenia Monfregola
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 287:44603-18. 2012
    ..MUNC13-4 is a RAB27A effector that coordinates exocytosis in hematopoietic cells, and its deficiency is associated with the human ..
  35. doi Slp2-a controls renal epithelial cell size through regulation of Rap-ezrin signaling independently of Rab27
    Takao Yasuda
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi 980 8578, Japan
    J Cell Sci 127:557-70. 2014
    ..Our findings indicate that Slp2-a controls renal cell size through regulation of Rap-ezrin signaling independently of Rab27. ..
  36. pmc Rab27A regulates transport of cell surface receptors modulating multinucleation and lysosome-related organelles in osteoclasts
    Megumi Shimada-Sugawara
    1 Division of Dental Pharmacology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852 8588, Japan 2 Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852 8588, Japan
    Sci Rep 5:9620. 2015
    b>Rab27A regulates transport of lysosome-related organelles (LROs) and release of secretory granules in various types of cells...
  37. doi Rab27a GTPase modulates L-type Ca2+ channel function via interaction with the II-III linker of CaV1.3 subunit
    Nadine Reichhart
    Experimental Ophthalmology, Eye Hospital, University Medical Center Regensburg, Regensburg, Germany Experimental Ophthalmology, Eye Hospital, Charite University Medicine, Campus Virchow Clinic, Berlin, Germany
    Cell Signal 27:2231-40. 2015
    In a variety of cells, secretory processes require the activation of both Rab27a and L-type channels of the Ca(V)1.3 subtype. In the retinal pigment epithelium (RPE), Rab27a and Ca(V)1...
  38. doi PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a
    Mami Yamaoka
    Department of Pharmacology, Oita University Faculty of Medicine, 1 1 Idaigaoka, Hasama, Yufu, Oita 879 5593, Japan
    J Cell Sci 129:637-49. 2016
    ..Here, we searched for proteins that interacted with the Rab27a GTPase-activating protein (GAP) EPI64 (also known as TBC1D10A) and identified the Arf6 guanine-nucleotide-exchange ..
  39. pmc Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion
    Yanan Hou
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA
    Biochem Biophys Res Commun 471:610-5. 2016
    ..g. similar small G proteins, such as Rab3D and Rab27A, and putative downstream effectors...
  40. doi MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A
    Cui Chang
    Department of Pharmaceutical Science, Shenzhen People s Hospital, Second Clinical College, Jinan University, Shenzhen 518020, China
    Gene . 2016
    ..The results of a luciferase assay showed that miR-134-3p silenced RAB27A by binding to the 3'-UTR of RAB27A mRNA...
  41. ncbi Missense mutations in the globular tail of myosin-Va in dilute mice partially impair binding of Slac2-a/melanophilin
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Cell Sci 117:583-91. 2004
    ..GT) of myosin-Va partially impair the binding of Slac2-a/melanophilin, a linker protein between myosin-Va and Rab27A on the melanosome...
  42. doi Lysosomal exocytosis in Schwann cells contributes to axon remyelination
    Gang Chen
    Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, China
    Glia 60:295-305. 2012
    ..Downregulation of Rab27a, a small GTPase required for the trafficking of the secretory lysosomes to the plasma membrane, largely blocked ..
  43. pmc The risk of hemophagocytic lymphohistiocytosis in Hermansky-Pudlak syndrome type 2
    Birthe Jessen
    Centre of Chronic Immunodeficiency, University Medical Center Freiburg and University of Freiburg, Breisacher Strasse 117, Freiburg, Germany
    Blood 121:2943-51. 2013
    ..Because that patient also carried a potentially contributing heterozygous RAB27A mutation, the risk for HLH in HPS2 remains unclear...
  44. doi Distinct and opposing roles for Rab27a/Mlph/MyoVa and Rab27b/Munc13-4 in mast cell secretion
    Rajesh K Singh
    Molecular Medicine, National Heart and Lung Institute, Imperial College London, London, UK
    FEBS J 280:892-903. 2013
    ..bone marrow-derived mast cells (BMMC) from Rab27-deficient mutant mice, we found that, in contrast to Rab27b, Rab27a primarily plays an inhibitory role in regulating degranulation...
  45. doi Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression
    Angélique Bobrie
    Institut Curie Centre de Recherche, INSERM U932, Paris, France
    Cancer Res 72:4920-30. 2012
    ..The specific roles of exosomes secreted in the tumor microenvironment, however, is unclear. The small GTPases RAB27A and RAB27B regulate exocytosis of multivesicular endosomes, which lead to exosome secretion, in human HeLa cells...
  46. pmc Rab27a-mediated protease release regulates neutrophil recruitment by allowing uropod detachment
    Rajesh K Singh
    Molecular Medicine, National Heart and Lung Institute NHLI, Imperial College, London, UK
    J Cell Sci 125:1652-6. 2012
    ..Here, we report that this process is regulated by the Rab27a GTPase, a protein known to control granule exocytosis...
  47. pmc Conditional ablation of the choroideremia gene causes age-related changes in mouse retinal pigment epithelium
    Silene T Wavre-Shapton
    Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, London, United Kingdom
    PLoS ONE 8:e57769. 2013
    ..Furthermore, the striking similarities between the present observations and some of the phenotypes reported in age-related macular degeneration (AMD) suggest that membrane traffic defects may contribute to the pathogenesis of AMD...
  48. doi Distinct severity of HLH in both human and murine mutants with complete loss of cytotoxic effector PRF1, RAB27A, and STX11
    Fernando E Sepulveda
    Inserm U768, Paris, France
    Blood 121:595-603. 2013
    ..In a cohort of HLH patients with genetic abnormalities expected to result in the complete absence of perforin, Rab27a, or syntaxin-11, we found that disease severity as determined by age at HLH onset differed significantly, with a ..
  49. ncbi The regulation of platelet-dense granules by Rab27a in the ashen mouse, a model of Hermansky-Pudlak and Griscelli syndromes, is granule-specific and dependent on genetic background
    Edward K Novak
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Blood 100:128-35. 2002
    ..prolonged bleeding times, and platelet storage pool deficiency due to a mutation which abrogates expression of the Rab27a protein...
  50. pmc Rab27b regulates exocytosis of secretory vesicles in acinar epithelial cells from the lacrimal gland
    Lilian Chiang
    School of Pharmacy, University of Southern California, Los Angeles, CA 90033, USA
    Am J Physiol Cell Physiol 301:C507-21. 2011
    ..of lacrimal glands from Rab27b(-/-) and Rab27(ash/ash)/Rab27b(-/-) mice, but not ashen mice deficient in Rab27a, showed changes in abundance and organization of secretory vesicles, further confirming a role for this protein in ..
  51. pmc Rab27a targeting to melanosomes requires nucleotide exchange but not effector binding
    Abul K Tarafder
    Molecular Medicine, National Heart and Lung Institute NHLI, Imperial College London, London SW7 2AZ, UK
    Traffic 12:1056-66. 2011
    ..Here, we address these issues using Rab27a targeting to melanosomes as a model system...
  52. pmc Cyclosporine-resistant, Rab27a-independent mobilization of intracellular preformed CD40 ligand mediates antigen-specific T cell help in vitro
    Yoshinobu Koguchi
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    J Immunol 187:626-34. 2011
    ..selective activation of cognate but not bystander APCs in vitro and that mobilization of pCD40L does not depend on Rab27a, which is required for mobilization of lytic granules...
  53. pmc Increased survival and reduced neutrophil infiltration of the liver in Rab27a- but not Munc13-4-deficient mice in lipopolysaccharide-induced systemic inflammation
    Jennifer L Johnson
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Infect Immun 79:3607-18. 2011
    Genetic defects in the Rab27a or Munc13-4 gene lead to immunodeficiencies in humans, characterized by frequent viral and bacterial infections...
  54. ncbi A role for Rab27b in NF-E2-dependent pathways of platelet formation
    Sanjay Tiwari
    Dana Farber Cancer Institute, One Jimmy Fund Way, Boston, MA 02115, USA
    Blood 102:3970-9. 2003
    ..limited number of Rab proteins with defective membrane association in gm/gm megakaryocytes prominently includes Rab27a and Rab27b...
  55. pmc Rapid degradation of dominant-negative Rab27 proteins in vivo precludes their use in transgenic mouse models
    Jose S Ramalho
    Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, Sir Alexander Fleming Building, Exhibition Road, London, SW7 2AZ, UK
    BMC Cell Biol 3:26. 2002
    ..We have previously reported that Rab27a is the Rab most affected in CHM lymphoblasts and hypothesised that the selective dysfunction of Rab27a (and ..
  56. ncbi Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 279:13065-75. 2004
    ..in the regulation of secretory vesicle exocytosis, however, recently both proteins have been shown to bind Rab27A in vitro in preference to Rab3A (Fukuda, M. (2003) J. Biol. Chem...
  57. ncbi Synaptotagmin-like protein 5: a novel Rab27A effector with C-terminal tandem C2 domains
    Taruho S Kuroda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem Biophys Res Commun 293:899-906. 2002
    ..We previously showed that the SHD functions as a binding site for Rab27A, which is associated with human hemophagocytic syndrome (Griscelli syndrome) [J. Biol. Chem. 277 (2002) 9212; J...
  58. pmc MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes
    Aziz El-Amraoui
    Unité de Génétique des Déficits Sensoriels, CNRS URA 1968, Institut Pasteur, 25 rue du Dr Roux, F 5724 Paris cedex 15, France
    EMBO Rep 3:463-70. 2002
    ..protein that we have named MyRIP (myosin-VIIa- and Rab-interacting protein), since it also binds to Rab27A in a GTP-dependent manner...
  59. ncbi Modifier genes in mice and humans
    J H Nadeau
    Department of Genetics, Case Western Reserve University School of Medicine and Center for Human Genetics, University Hospital of Cleveland, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA
    Nat Rev Genet 2:165-74. 2001
    ....
  60. pmc Chromosomal mapping, gene structure and characterization of the human and murine RAB27B gene
    J S Ramalho
    Cell and Molecular Biology, Division of Biomedical Sciences, Imperial College School of Medicine, Sir Alexander Fleming Building, London SW7 2AZ, UK
    BMC Genet 2:2. 2001
    Rab GTPases are regulators of intracellular membrane traffic. The Rab27 subfamily consists of Rab27a and Rab27b. Rab27a has been recently implicated in Griscelli Disease, a disease combining partial albinism with severe immunodeficiency...
  61. ncbi Relationship between a retroviral germ line reintegration and a new mutation at the ashen locus in B10.F mice. Retroviral integration and an ashen mutation
    W Y Langdon
    Virology 133:183-90. 1984
    ..The coat-color mutation was found to be allelic to the ashen locus which is closely linked to the dilute locus on chromosome 9...
  62. pmc Interaction of the murine dilute suppressor gene (dsu) with fourteen coat color mutations
    K J Moore
    Mammalian Genetics Laboratory, NCI Frederick Cancer Research Facility, Maryland 21701
    Genetics 125:421-30. 1990
    ..shown to suppress the dilute coat color phenotypes of mice homozygous for the dilute (d), leaden (ln), and ashen (ash) mutations...
  63. ncbi Identification of EPI64 as a GTPase-activating protein specific for Rab27A
    Takashi Itoh
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 281:31823-31. 2006
    Small GTPase Rab27A plays a pivotal role in melanosome transport in melanocytes and in secretion by various secreting cells...
  64. doi Microphthalmia-associated transcription factor regulates RAB27A gene expression and controls melanosome transport
    Christine Chiaverini
    INSERM U895, Biologie et Pathologies des Cellules Mélanocytaires de la Pigmentation Cutanée au Mélanome 28, Avenue de Valombrose, 06107 Nice Cedex 2, France
    J Biol Chem 283:12635-42. 2008
    ..factor (MITF) silencing induces melanosome gathering around the nucleus and causes the relocalization of Rab27A, Slac2a-Mlph, and Myo5a that control the transport of melanosomes on the actin network...
  65. ncbi Doc2 alpha and Munc13-4 regulate Ca(2+) -dependent secretory lysosome exocytosis in mast cells
    Hironori Higashio
    Department of Biochemistry, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan
    J Immunol 180:4774-84. 2008
    ..These results suggest that the Doc2alpha-Muunc13-4 system regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells...
  66. ncbi Functional analysis of Slac2-c/MyRIP as a linker protein between melanosomes and myosin VIIa
    Taruho S Kuroda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 280:28015-22. 2005
    Slac2-c/MyRIP, an in vitro Rab27A- and myosin Va/VIIa-binding protein, has recently been proposed to regulate retinal melanosome transport in retinal pigment epithelium cells by directly linking melanosome-bound Rab27A and myosin VIIa; ..
  67. pmc Purification, crystallization and preliminary X-ray crystallographic analysis of Rab27a GTPase in complex with exophilin4/Slp2-a effector
    Leonard M G Chavas
    Faculty of Life Sciences, The University of Manchester, Michael Smith Building, Oxford Road, Manchester, Lancashire M13 9PT, England
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:599-601. 2008
    ..Among Rab GTPases, the Rab27 subfamily, which comprises Rab27a and Rab27b, controls the proper targeting of secretory vesicles to the plasma membrane...
  68. pmc Murine leukemia virus spreading in mice impaired in the biogenesis of secretory lysosomes and Ca2+-regulated exocytosis
    Wai Tsing Chan
    Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, United States of America
    PLoS ONE 3:e2713. 2008
    ..Retroviruses have been observed to bud intracellularly into multivesicular bodies (MVB), in addition to the plasma membrane. Release from MVB is thought to occur by Ca(2+)-regulated fusion with the plasma membrane...
  69. pmc Rab8 regulates the actin-based movement of melanosomes
    Marion L Chabrillat
    Unité de Morphogenèse et Signalisation Cellulaires, Institut Curie, Centre National de la Recherche Scientifique Unité Mixte de Recherche 144, Paris Cedex, France
    Mol Biol Cell 16:1640-50. 2005
    ..Together, our observations suggest that Rab8 is involved in regulating the actin-dependent movement of melanosomes...
  70. pmc Recruitment of Rab27a to phagosomes controls microbial antigen cross-presentation by dendritic cells
    Seong Hyun Kim
    Department of Dermatology, Brigham and Women s Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Infect Immun 76:5373-80. 2008
    ..The small GTPase Rab27a was recently implicated in antigen cross-presentation and was rapidly recruited to phagosomes only when Ig was ..
  71. pmc Kinetics of Rab27a-dependent actions on vesicle docking and priming in pancreatic beta-cells
    Matthew J Merrins
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109 0622, USA
    J Physiol 586:5367-81. 2008
    The small GTPase Rab27a, along with the isoforms of Rab3, is present on insulin secretory granules and has been implicated in regulation of Ca(2+)-triggered exocytosis...
  72. ncbi Characterization of melanosomes in murine Hermansky-Pudlak syndrome: mechanisms of hypopigmentation
    Thuyen Nguyen
    Department of Dermatology, Veterans Affairs Medical Center, University of California, San Francisco, California 94121, USA
    J Invest Dermatol 122:452-60. 2004
    ..We demonstrate that all strains examined here except for ashen have defects in morphogenesis, the most severely affected is sandy, muted, and buff followed by subtle gray...
  73. pmc Rab27a is required for human cytomegalovirus assembly
    Alberto Fraile-Ramos
    Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    PLoS ONE 5:e15318. 2010
    ..The mechanisms underlying these processes are not understood. Here we studied the role of Rab27a, a regulator of lysosome-related organelle transport, in HCMV production...
  74. doi Protein trafficking in immune cells
    Anat Benado
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Immunobiology 214:403-21. 2009
    ..Herein, we discuss these mechanisms in health and disease states...
  75. doi Glucose-induced translocation of coronin 3 regulates the retrograde transport of the secretory membrane in the pancreatic beta-cells
    Toshihide Kimura
    Department of Pharmacology, Oita University Faculty of Medicine, 1 1 Idaigaoka, Hasama, Yufu, Oita 879 5593, Japan
    Biochem Biophys Res Commun 395:318-23. 2010
    GTP-Rab27a is known to regulate insulin exocytosis. We have recently reported that coronin 3, which paradoxically binds GDP-Rab27a, participates in endocytosis of the insulin secretory membrane...
  76. pmc PtdIns3P and Rac direct the assembly of the NADPH oxidase on a novel, pre-phagosomal compartment during FcR-mediated phagocytosis in primary mouse neutrophils
    Karen E Anderson
    Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge, UK
    Blood 116:4978-89. 2010
    ..and activation is highly dependent on both PtdIns3P-p40(phox) and Rac2-p67(phox) interactions and delivery to the phagosome is regulated by Rab27a. These results define a novel pathway for oxidase assembly downstream of FcR-activation.
  77. pmc Distinct role of rab27a in granule movement at the plasma membrane and in the cytosol of NK cells
    Dongfang Liu
    Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA
    PLoS ONE 5:e12870. 2010
    ..Both mouse NK cells and a human NK cell line deficient in the small GTPase Rab27a were examined. The unbiased analysis of large datasets led to the following observations and conclusions...
  78. doi Rab27a and Rab27b are involved in stimulation-dependent RANKL release from secretory lysosomes in osteoblastic cells
    Yoshiaki Kariya
    Department of Pharmacy, University of Tokyo Hospital, Faculty of Medicine, University of Tokyo, Bunkyo ku, Tokyo, Japan
    J Bone Miner Res 26:689-703. 2011
    ..In this study we show that small GTPases Rab27a and Rab27b are involved in the stimulation-dependent RANKL release pathway in osteoblastic cells...
  79. ncbi The acroplaxome is the docking site of Golgi-derived myosin Va/Rab27a/b- containing proacrosomal vesicles in wild-type and Hrb mutant mouse spermatids
    Abraham L Kierszenbaum
    Department of Cell Biology and Anatomical Sciences, The Sophie Davis School of Biomedical Education City University of New York Medical School, New York, New York 10031, USA
    Biol Reprod 70:1400-10. 2004
    ..We also show that the actin-based motor protein myosin Va and its receptor, Rab27a/b, known to be involved in vesicle transport, are present in the Golgi and Golgi-derived proacrosomal vesicles in ..
  80. pmc Munc13-4 restricts motility of Rab27a-expressing vesicles to facilitate lipopolysaccharide-induced priming of exocytosis in neutrophils
    Jennifer L Johnson
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 286:5647-56. 2011
    ..The small GTPase Rab27a and its effector Munc13-4 regulate exocytosis in hematopoietic cells...
  81. pmc The Slc35d3 gene, encoding an orphan nucleotide sugar transporter, regulates platelet-dense granules
    Sreenivasulu Chintala
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Blood 109:1533-40. 2007
    ..Previous studies produced contradictory findings for the function of the murine ashen (Rab27a) gene in platelet-dense granules...

Research Grants1

  1. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..