Ppp1r9b

Summary

Gene Symbol: Ppp1r9b
Description: protein phosphatase 1, regulatory subunit 9B
Alias: SPL, Spn, neurabin-2, neurabin-II, spinophilin
Species: mouse

Top Publications

  1. pmc Spinophilin regulates the formation and function of dendritic spines
    J Feng
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 97:9287-92. 2000
  2. ncbi A protein phosphatase-1gamma1 isoform selectivity determinant in dendritic spine-associated neurabin
    Leigh C Carmody
    Department of Molecular Physiology and Biophysics, The Center for Molecular Neuroscience, and The Vanderbilt Kennedy Center for Research on Human Development, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0615, USA
    J Biol Chem 279:21714-23. 2004
  3. pmc Spinophilin facilitates dephosphorylation of doublecortin by PP1 to mediate microtubule bundling at the axonal wrist
    Stephanie L Bielas
    Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 129:579-91. 2007
  4. ncbi Distinct roles for spinophilin and neurabin in dopamine-mediated plasticity
    P B Allen
    Department of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT 06508, USA
    Neuroscience 140:897-911. 2006
  5. ncbi The Rho-specific GEF Lfc interacts with neurabin and spinophilin to regulate dendritic spine morphology
    Xiaozhou P Ryan
    Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Neuron 47:85-100. 2005
  6. pmc Spinophilin/neurabin reciprocally regulate signaling intensity by G protein-coupled receptors
    Xinhua Wang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9040, USA
    EMBO J 26:2768-76. 2007
  7. ncbi Spinophilin stabilizes cell surface expression of alpha 2B-adrenergic receptors
    Ashley E Brady
    Department of Pharmacology, Center for Molecular Neuroscience, Vanderbilt University Medical Center, Nashville, Tennessee 37232 6600, USA
    J Biol Chem 278:32405-12. 2003
  8. ncbi Spinophilin blocks arrestin actions in vitro and in vivo at G protein-coupled receptors
    Qin Wang
    Department of Pharmacology and Center of Molecular Neuroscience, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Science 304:1940-4. 2004
  9. pmc The Cytoskeletal Adapter Protein Spinophilin Regulates Invadopodia Dynamics and Tumor Cell Invasion in Glioblastoma
    Mujeeburahiman Cheerathodi
    University of Texas MD Anderson Cancer Center, Houston, Texas
    Mol Cancer Res 14:1277-1287. 2016
  10. pmc A newly identified complex of spinophilin and the tyrosine phosphatase, SHP-1, modulates platelet activation by regulating G protein-dependent signaling
    Peisong Ma
    Department of Medicine and Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Blood 119:1935-45. 2012

Scientific Experts

  • P B Allen
  • Anthony J Baucum
  • Miki Tsukada
  • J Feng
  • Paul Greengard
  • Qin Wang
  • Peisong Ma
  • Irene Ferrer
  • Amancio Carnero
  • Christopher Cottingham
  • Volkmar Gross
  • Mujeeburahiman Cheerathodi
  • Ruizhi Wang
  • David Y Chiang
  • Lawrence F Brass
  • Andrew J Sinnamon
  • J P Palavicini
  • Lee E Limbird
  • Marie Futter
  • Ken Uematsu
  • Angus C Nairn
  • Inigo Ruiz de Azua
  • Daria Petzhold
  • Andrey C da Costa Goncalves
  • Yolanda Cecilia
  • Carmen Blanco-Aparicio
  • Marta Canamero
  • Kai Jiao
  • Roujian Lu
  • Soumitra Rajagopal
  • Ashley E Brady
  • Leigh C Carmody
  • Jens Jordan
  • Jens Tank
  • Roger J Colbran
  • Maik Gollasch
  • Friedrich C Luft
  • Subodh Nag
  • Long Jun Wu
  • Joanna J Charlton
  • Seiji Fujii
  • Ona Bloom
  • Jianmin Xu
  • Andrey C da Costa-Goncalves
  • Xinhua Wang
  • Stephanie L Bielas
  • Akinori Nishi
  • Xiaozhou P Ryan
  • Bryan A Ballif
  • Paola A Guerrero
  • Zhihua Chen
  • Gonzalo M Rivera
  • Leung K Tang
  • Joseph H McCarty
  • Frederick F Lang
  • Julia Popp
  • John E Morales
  • Naze G Avci
  • Hongjie Wang
  • Shaohua Xu
  • Ivan Carrera
  • Kristy Ou
  • Hong Jiang
  • David P Siderovski
  • Madepalli K Lakshmana
  • Qiongling Wang
  • Ann P Quick
  • Xander H T Wehrens
  • Dobromir Dobrev
  • Na Li
  • Katherina M Alsina
  • Niels Voigt
  • Guoliang Wang
  • Julia O Reynolds
  • Darlene Skapura
  • Ryan T Terry-Lorenzo
  • M K Lakshmana
  • D E Kang
  • S Xu
  • H Wang
  • J S Rao
  • E Bianchi
  • Timothy J Stalker
  • Rachel Signarvic
  • Ken ichiro Nakajima
  • William Jou
  • Oksana Gavrilova
  • Marcin Cieslak
  • Richard R Neubig
  • Jurgen Wess

Detail Information

Publications36

  1. pmc Spinophilin regulates the formation and function of dendritic spines
    J Feng
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 97:9287-92. 2000
    b>Spinophilin, a protein that interacts with actin and protein phosphatase-1, is highly enriched in dendritic spines...
  2. ncbi A protein phosphatase-1gamma1 isoform selectivity determinant in dendritic spine-associated neurabin
    Leigh C Carmody
    Department of Molecular Physiology and Biophysics, The Center for Molecular Neuroscience, and The Vanderbilt Kennedy Center for Research on Human Development, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0615, USA
    J Biol Chem 279:21714-23. 2004
    ..studies from brain and muscle extracts demonstrated that PP1gamma1 selectively interacts with spinophilin and neurabin, F-actin-targeting proteins, whereas PP1beta selectively interacted with G(M)/R(GL), the striated-..
  3. pmc Spinophilin facilitates dephosphorylation of doublecortin by PP1 to mediate microtubule bundling at the axonal wrist
    Stephanie L Bielas
    Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 129:579-91. 2007
    ..We find that Spinophilin, a Protein-phosphatase 1 (PP1) targeting protein, is responsible for the dephosphorylation of the MAP ..
  4. ncbi Distinct roles for spinophilin and neurabin in dopamine-mediated plasticity
    P B Allen
    Department of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT 06508, USA
    Neuroscience 140:897-911. 2006
    ..protein phosphatase 1 activity include interactions with the structurally related cytoskeletal elements spinophilin and neurabin, synaptic scaffolding proteins that are highly enriched in dendritic spines...
  5. ncbi The Rho-specific GEF Lfc interacts with neurabin and spinophilin to regulate dendritic spine morphology
    Xiaozhou P Ryan
    Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Neuron 47:85-100. 2005
    Neurabin and spinophilin are homologous protein phosphatase 1 and actin binding proteins that regulate dendritic spine function...
  6. pmc Spinophilin/neurabin reciprocally regulate signaling intensity by G protein-coupled receptors
    Xinhua Wang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9040, USA
    EMBO J 26:2768-76. 2007
    b>Spinophilin (SPL) and neurabin (NRB) are structurally similar scaffolding proteins with several protein binding modules, including actin and PP1 binding motifs and PDZ and coiled-coil domains...
  7. ncbi Spinophilin stabilizes cell surface expression of alpha 2B-adrenergic receptors
    Ashley E Brady
    Department of Pharmacology, Center for Molecular Neuroscience, Vanderbilt University Medical Center, Nashville, Tennessee 37232 6600, USA
    J Biol Chem 278:32405-12. 2003
    ..The third intracellular loops of the alpha 2A, alpha 2B, and alpha 2C-AR subtypes interact with spinophilin, a multidomain protein that, like the three alpha 2-AR subtypes, is enriched at the basolateral surface of ..
  8. ncbi Spinophilin blocks arrestin actions in vitro and in vivo at G protein-coupled receptors
    Qin Wang
    Department of Pharmacology and Center of Molecular Neuroscience, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Science 304:1940-4. 2004
    ..We report that the multidomain protein, spinophilin, antagonizes these multiple arrestin functions...
  9. pmc The Cytoskeletal Adapter Protein Spinophilin Regulates Invadopodia Dynamics and Tumor Cell Invasion in Glioblastoma
    Mujeeburahiman Cheerathodi
    University of Texas MD Anderson Cancer Center, Houston, Texas
    Mol Cancer Res 14:1277-1287. 2016
    ..a novel adhesion and signaling pathway comprised of the integrin αvβ8 and its intracellular binding partner, Spinophilin (Spn), which regulates glioblastoma cell invasion in the brain microenvironment...
  10. pmc A newly identified complex of spinophilin and the tyrosine phosphatase, SHP-1, modulates platelet activation by regulating G protein-dependent signaling
    Peisong Ma
    Department of Medicine and Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Blood 119:1935-45. 2012
    ..Here, we describe a novel complex comprising the scaffold protein, spinophilin (SPL), and the tyrosine phosphatase, SHP-1, and show that it can modulate platelet activation by sequestering ..
  11. pmc Age-dependent targeting of protein phosphatase 1 to Ca2+/calmodulin-dependent protein kinase II by spinophilin in mouse striatum
    Anthony J Baucum
    Department of Molecular Physiology and Biophysics, Vanderbilt Kennedy Center, Center for Molecular Neuroscience, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America
    PLoS ONE 7:e31554. 2012
    ..b>Spinophilin is an F-actin- and protein phosphatase 1 (PP1)-binding protein that targets PP1 to multiple downstream ..
  12. pmc RanBP9 aggravates synaptic damage in the mouse brain and is inversely correlated to spinophilin levels in Alzheimer's brain synaptosomes
    J P Palavicini
    Section of Neurobiology, Torrey Pines Institute for Molecular Studies, Port Saint Lucie, FL 34987 2352, USA
    Cell Death Dis 4:e667. 2013
    ..In the present study, we found striking reduction of spinophilin protein levels when RanBP9 is overexpressed...
  13. pmc Impaired local regulation of ryanodine receptor type 2 by protein phosphatase 1 promotes atrial fibrillation
    David Y Chiang
    Cardiovascular Research Institute, Baylor College of Medicine, Houston, TX, USA Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USA Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA
    Cardiovasc Res 103:178-87. 2014
    ..This is due to a lack of investigation into the local, rather than global, regulation of PP1 on different subcellular targets such as ryanodine receptor type 2 (RyR2), especially in AF...
  14. pmc COPS5 protein overexpression increases amyloid plaque burden, decreases spinophilin-immunoreactive puncta, and exacerbates learning and memory deficits in the mouse brain
    Ruizhi Wang
    From the Section of Neurobiology, Torrey Pines Institute for Molecular Studies, Port Saint Lucie, Florida 34987
    J Biol Chem 290:9299-309. 2015
    ..Furthermore, COPS5 overexpression reduced spinophilin in both the cortex (19%, p < 0.05) and the hippocampus (20%, p < 0...
  15. pmc Modulating platelet reactivity through control of RGS18 availability
    Peisong Ma
    Departments of Medicine and Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA and
    Blood 126:2611-20. 2015
    ..that a critical node in the G-protein signaling network in platelets is formed by a scaffold protein, spinophilin (SPL), the tyrosine phosphatase, Src homology region 2 domain-containing phosphatase-1 (SHP-1), and the ..
  16. ncbi The neuronal actin-binding proteins, neurabin I and neurabin II, recruit specific isoforms of protein phosphatase-1 catalytic subunits
    Ryan T Terry-Lorenzo
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:27716-24. 2002
    ..Immunoprecipitation of neurabin I and neurabin II/spinophilin from rat brain extracts sedimented PP1gamma1 and PP1alpha but not PP1beta...
  17. pmc Cross-talk from β-adrenergic receptors modulates α2A-adrenergic receptor endocytosis in sympathetic neurons via protein kinase A and spinophilin
    Christopher Cottingham
    From the Departments of Cell, Developmental, and Integrative Biology and
    J Biol Chem 288:29193-205. 2013
    ..from the AR literature and our own extensive studies on regulation of α2AARs by the scaffolding protein spinophilin have illuminated a potential novel mechanism for cross-talk from β to α2ARs...
  18. pmc Differential association of postsynaptic signaling protein complexes in striatum and hippocampus
    Anthony J Baucum
    Department of Molecular Physiology and Biophysics, Vanderbilt Kennedy Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    J Neurochem 124:490-501. 2013
    ..calcium/calmodulin-dependent protein kinase II (CaMKII), a highly abundant serine/threonine kinase, and spinophilin, a F-actin and protein phosphatase 1 (PP1) binding protein, were significantly lower in striatal extracts, as ..
  19. pmc Spinophilin as a novel regulator of M3 muscarinic receptor-mediated insulin release in vitro and in vivo
    Inigo Ruiz de Azua
    Molecular Signaling Section, Mouse Metabolic Core Facility, Laboratory of Bioorganic Chemistry, NIH NIDDK, Bldg 8A, Rm B1A 05, 8 Center Dr MSC 0810 Bethesda, MD 20892 0810, USA
    FASEB J 26:4275-86. 2012
    b>Spinophilin (SPL), a multidomain scaffolding protein known to modulate the activity of different G-protein-coupled receptors, regulates various central nervous system (CNS) functions...
  20. doi Spinophilin is required for normal morphology, Ca(2+) homeostasis and contraction but dispensable for β-adrenergic stimulation of adult cardiomyocytes
    Daria Petzhold
    Max Delbrück Center for Molecular Medicine MDC, Robert Rossle Str 10, 13092, Berlin, Germany
    J Muscle Res Cell Motil 32:243-8. 2011
    b>Spinophilin (SPN) is a ubiquitously expressed scaffolding protein that interacts through several binding modules with a variety of target proteins...
  21. doi Spinophilin regulates central angiotensin II-mediated effect on blood pressure
    Andrey C da Costa Goncalves
    Leibniz Institute for Molecular Pharmacology, Berlin, Germany
    J Mol Med (Berl) 89:1219-29. 2011
    ..The underlining molecular mechanisms are largely unknown. Spinophilin (SPL) is a regulator of G protein-coupled receptor signaling...
  22. ncbi Spinophilin acts as a tumor suppressor by regulating Rb phosphorylation
    Irene Ferrer
    Experimental Therapeutics Programme, Instituto de Biomedicina de Sevilla HUVR, Sevilla, Spain
    Cell Cycle 10:2751-62. 2011
    The scaffold protein Spinophilin (SPN) is a regulatory subunit of phosphatase1a located at 17q21.33...
  23. doi Role of the multidomain protein spinophilin in blood pressure and cardiac function regulation
    Andrey C da Costa-Goncalves
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Hypertension 52:702-7. 2008
    b>Spinophilin controls intensity/duration of G protein-coupled receptor signaling and thereby influences synaptic activity. We hypothesize that spinophilin affects blood pressure through central mechanisms...
  24. ncbi Spinophilin loss contributes to tumorigenesis in vivo
    Irene Ferrer
    Experimental Therapeutics Program, Spanish National Cancer Research Center, Madrid, Spain
    Cell Cycle 10:1948-55. 2011
    The scaffold protein spinophilin (SPN, PPP1R9B) is a regulatory subunit of phosphatase-1a located at 17q21.31...
  25. pmc Scaffold proteins IRSp53 and spinophilin regulate localized Rac activation by T-lymphocyte invasion and metastasis protein 1 (TIAM1)
    Soumitra Rajagopal
    Molecular Oncology Research Institute, Tufts Medical Center, Tufts UniversitySackler School of Graduate Biomedical Sciences, Boston, Massachusetts 02111, USA
    J Biol Chem 285:18060-71. 2010
    ..express at least two Tiam1-interacting proteins, insulin receptor substrate protein 53 kDa (IRSp53) and spinophilin. We used fluorescent resonance energy transfer (FRET) to measure localized Rac activation associated with ..
  26. pmc Knockout of spinophilin, an endogenous antagonist of arrestin-dependent alpha2-adrenoceptor functions, enhances receptor-mediated antinociception yet does not eliminate sex-related differences
    Subodh Nag
    Department of Neurobiology and Neurotoxicology, Meharry Medical College, 1005 D B Todd Boulevard, Nashville, TN 37208, USA
    Behav Brain Res 197:457-61. 2009
    ..Agonist activation of the receptor enhances its association with spinophilin that antagonizes arrestin functions both by diminishing receptor phosphorylation by G-protein-coupled receptor ..
  27. doi Spinophilin inhibits the binding of RGS8 to M1-mAChR but enhances the regulatory function of RGS8
    Seiji Fujii
    Department of Bio Science, Faculty of Bio Science, Nagahama Institute of Bio Science and Technology, Nagahama shi, Shiga, Japan
    Biochem Biophys Res Commun 377:200-4. 2008
    ..Here, we identified spinophilin (SPL) as an RGS8-interacting protein...
  28. pmc Multiple actions of spinophilin regulate mu opioid receptor function
    Joanna J Charlton
    Department of Pharmacology, University of Crete, 71409 Heraklion, Crete, Greece
    Neuron 58:238-47. 2008
    b>Spinophilin, a dendritic spine-enriched scaffold protein, modulates synaptic transmission via multiple functions mediated by distinct domains of the protein. Here, we show that spinophilin is a key modulator of opiate action...
  29. pmc Spinophilin participates in information transfer at immunological synapses
    Ona Bloom
    Department of Cell Biology, Ludwig Institute for Cancer Research, New Haven, CT 06520, USA
    J Cell Biol 181:203-11. 2008
    ..One such protein is spinophilin, a scaffolding protein of neuronal dendritic spines that regulates synaptic transmission...
  30. doi Protein kinase A phosphorylation of spinophilin modulates its interaction with the alpha 2A-adrenergic receptor (AR) and alters temporal properties of alpha 2AAR internalization
    Jianmin Xu
    Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Biol Chem 283:14516-23. 2008
    b>Spinophilin plays critical roles in regulating trafficking and signaling of the alpha(2)-adrenergic receptor (AR) both in vitro and in vivo (Wang, Q., Zhao, J., Brady, A. E., Feng, J., Allen, P. B., Lefkowitz, R. J., Greengard, P...
  31. pmc Neurabin contributes to hippocampal long-term potentiation and contextual fear memory
    Long Jun Wu
    Department of Physiology, University of Toronto, Toronto, Onatrio, Canada
    PLoS ONE 3:e1407. 2008
    ..Our results indicate that neurabin regulates contextual fear memory and LTP in hippocampal CA1 pyramidal neurons...
  32. ncbi Regulation of spinophilin Ser94 phosphorylation in neostriatal neurons involves both DARPP-32-dependent and independent pathways
    Ken Uematsu
    Department of Physiology, Kurume University School of Medicine, 67 Asahi Machi, Kurume, Fukuoka, Japan
    J Neurochem 95:1642-52. 2005
    b>Spinophilin is a protein phosphatase-1 (PP-1)- and actin-binding protein that is enriched in dendritic spines...
  33. pmc Phosphorylation of spinophilin by ERK and cyclin-dependent PK 5 (Cdk5)
    Marie Futter
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:3489-94. 2005
    b>Spinophilin is a protein that binds to protein phosphatase-1 and actin and modulates excitatory synaptic transmission and dendritic spine morphology...
  34. ncbi Doublecortin association with actin filaments is regulated by neurabin II
    Miki Tsukada
    Max Planck Institute for Experimental Endocrinology, Feodor Lynen Strasse 7, D 30625 Hannover, Germany
    J Biol Chem 280:11361-8. 2005
    ..When cells were co-transfected with neurabin II, a protein that binds F-actin as well as Dcx, GFP-Dcx and to an even greater extent GFP-Dcx(S47E) became ..
  35. ncbi Identification of neurabin II as a novel doublecortin interacting protein
    Miki Tsukada
    Max Planck Institute for Experimental Endocrinology, Feodor Lynen Strasse 7, Hannover 30625, Germany
    Mech Dev 120:1033-43. 2003
    ..Using a yeast two-hybrid screen with Dcx as bait, we have isolated neurabin II/spinophilin, an F-actin binding protein known to play a role in dendritic spine formation...
  36. pmc Impaired conditioned taste aversion learning in spinophilin knockout mice
    C A Stafstrom-Davis
    Program in Neuroscience, Florida State University, Tallahassee, Florida 32306 4340 USA
    Learn Mem 8:272-8. 2001
    Plasticity in dendritic spines may underlie learning and memory. Spinophilin, a protein enriched in dendritic spines, has the properties of a scaffolding protein and is believed to regulate actin cytoskeletal dynamics affecting dendritic ..