Ppp1r12a

Summary

Gene Symbol: Ppp1r12a
Description: protein phosphatase 1, regulatory (inhibitor) subunit 12A
Alias: 1200015F06Rik, 5730577I22Rik, AA792106, AV099298, D10Ertd625e, Mypt1, protein phosphatase 1 regulatory subunit 12A, myosin phosphatase target subunit 1, myosin phosphatase-targeting subunit 1
Species: mouse

Top Publications

  1. ncbi Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene
    H Machida
    First Department of Internal Medicine, University School of Medicine, Tsu, Mie, Japan
    Biochim Biophys Acta 1517:424-9. 2001
  2. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
  3. pmc Myosin phosphatase target subunit 1 (MYPT1) regulates the contraction and relaxation of vascular smooth muscle and maintains blood pressure
    Yan Ning Qiao
    From the Key Laboratory of MOE for Modern Teaching Technology, Shaanxi Normal University, Xi an 710062, China, Model Animal Research Center and MOE Key Laboratory of Animal Models of Disease, Nanjing University, Nanjing 210061, China
    J Biol Chem 289:22512-23. 2014
  4. pmc Myosin phosphatase modulates the cardiac cell fate by regulating the subcellular localization of Nkx2.5 in a Wnt/Rho-associated protein kinase-dependent pathway
    Tammy Ryan
    Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada
    Circ Res 112:257-66. 2013
  5. pmc Constitutive phosphorylation of myosin phosphatase targeting subunit-1 in smooth muscle
    Ming Ho Tsai
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA
    J Physiol 592:3031-51. 2014
  6. pmc TRA2β controls Mypt1 exon 24 splicing in the developmental maturation of mouse mesenteric artery smooth muscle
    Xiaoxu Zheng
    Division of Cardiovascular Medicine, School of Medicine, University of Maryland, Baltimore, Maryland
    Am J Physiol Cell Physiol 308:C289-96. 2015
  7. pmc In vivo roles for myosin phosphatase targeting subunit-1 phosphorylation sites T694 and T852 in bladder smooth muscle contraction
    Cai Ping Chen
    Model Animal Research Center and MOE Key Laboratory of Animal Models of Disease, Nanjing University, Nanjing, China
    J Physiol 593:681-700. 2015
  8. pmc Redox signaling and splicing dependent change in myosin phosphatase underlie early versus late changes in NO vasodilator reserve in a mouse LPS model of sepsis
    John J Reho
    Department of Medicine, Divisions of Cardiovascular Medicine and Nephrology, University of Maryland, Baltimore, Maryland
    Am J Physiol Heart Circ Physiol 308:H1039-50. 2015
  9. pmc DAAM1 and DAAM2 are co-required for myocardial maturation and sarcomere assembly
    Rieko Ajima
    Mammalian Development Laboratory, National Institute of Genetics, Mishima 411 8540, Japan
    Dev Biol 408:126-39. 2015
  10. pmc A splice variant of the myosin phosphatase regulatory subunit tunes arterial reactivity and suppresses response to salt loading
    John J Reho
    Department of Medicine, Divisions of Cardiovascular Medicine and Nephrology, University of Maryland Baltimore, Baltimore, Maryland
    Am J Physiol Heart Circ Physiol 310:H1715-24. 2016

Scientific Experts

  • Kevin B Atkins
  • Junji Yamauchi
  • John J Reho
  • Wei Qi He
  • Steven A Fisher
  • James T Stull
  • Kristine E Kamm
  • Cai Ping Chen
  • Yan Ning Qiao
  • Xiaoxu Zheng
  • Juan Min Zha
  • Pei Wang
  • Min Sheng Zhu
  • Cheng Hai Zhang
  • Laureano D Asico
  • Rieko Ajima
  • Wei Zhao
  • Ming Ho Tsai
  • Chen Chen
  • Brunhilde Wirth
  • Xiao Yang
  • Ya Jing Peng
  • Jung Chien Cheng
  • David J Hartshorne
  • Tammy Ryan
  • Kang Fu
  • B P Bhetwal
  • Deqiang Li
  • Jian Huang
  • Yaopan Mao
  • Hiroshi Kamijo
  • Beata Lontay
  • Dou Dou
  • Rowena Suriben
  • Jee In Kim
  • Nicole Harb
  • Supriya Shukla
  • Win D Cheung
  • Daisuke Umeda
  • Yusuke Mizuno
  • Maribel Parra
  • Haruhiko Tokuda
  • Lubomir T Lubomirov
  • Masaaki Ito
  • Ryuji Okamoto
  • Yue Wu
  • Kenta Kobayashi
  • Doreswamy Kenchegowda
  • Min Tao
  • Qian Lin
  • Hua Shan Li
  • Yi Tang Wang
  • Raymond Habas
  • Ethan David Cohen
  • Jay Christian Helt
  • Ye Wang
  • Xi He
  • Lino Tessarollo
  • Tao Tao
  • Xin Chen
  • Masa aki Nakaya
  • Ning Gao
  • Edward E Morrisey
  • Jie Sun
  • Terry P Yamaguchi
  • Joseph A Bisson
  • Yun Qian Gao
  • Lin Zhang
  • Audrey N Chang
  • Ji min Gao
  • Minsheng Zhu
  • Weiqi He
  • H Lee Sweeney
  • Yan Ze Wu
  • Michael Shelton
  • Pier Lorenzo Puri
  • Barbora Malecova
  • Jean Philippe Lambert
  • Hui Pin Cheng
  • Daniel Figeys
  • Chao Jun Li
  • Meei Jyh Jiang
  • Sophie Boisvenue
  • Ilona S Skerjanc
  • Marc Ruel
  • I Ching Tsai
  • Salah Baker
  • Bhupal P Bhetwal
  • H Machida
  • Brian A Perrino

Detail Information

Publications38

  1. ncbi Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene
    H Machida
    First Department of Internal Medicine, University School of Medicine, Tsu, Mie, Japan
    Biochim Biophys Acta 1517:424-9. 2001
    We have cloned and sequenced a 5 kb genomic fragment in the 5'-flanking region of the human myosin phosphatase target subunit 1. The transcription initiation site (+1) was 268 bp upstream from the translation start site...
  2. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
    ..Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase...
  3. pmc Myosin phosphatase target subunit 1 (MYPT1) regulates the contraction and relaxation of vascular smooth muscle and maintains blood pressure
    Yan Ning Qiao
    From the Key Laboratory of MOE for Modern Teaching Technology, Shaanxi Normal University, Xi an 710062, China, Model Animal Research Center and MOE Key Laboratory of Animal Models of Disease, Nanjing University, Nanjing 210061, China
    J Biol Chem 289:22512-23. 2014
    Myosin light chain phosphatase with its regulatory subunit, myosin phosphatase target subunit 1 (MYPT1) modulates Ca(2+)-dependent phosphorylation of myosin light chain by myosin light chain kinase, which is essential for smooth muscle ..
  4. pmc Myosin phosphatase modulates the cardiac cell fate by regulating the subcellular localization of Nkx2.5 in a Wnt/Rho-associated protein kinase-dependent pathway
    Tammy Ryan
    Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada
    Circ Res 112:257-66. 2013
    ..5 has been implicated in the regulation of many cellular processes that ultimately contribute to cardiomyogenesis and morphogenesis of the mature heart, relatively little is known about how it is regulated at a functional level...
  5. pmc Constitutive phosphorylation of myosin phosphatase targeting subunit-1 in smooth muscle
    Ming Ho Tsai
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA
    J Physiol 592:3031-51. 2014
    ..We have investigated the physiological role of the MLCP regulatory subunit MYPT1 in bladder smooth muscle containing a smooth muscle-specific deletion of MYPT1 in adult mice...
  6. pmc TRA2β controls Mypt1 exon 24 splicing in the developmental maturation of mouse mesenteric artery smooth muscle
    Xiaoxu Zheng
    Division of Cardiovascular Medicine, School of Medicine, University of Maryland, Baltimore, Maryland
    Am J Physiol Cell Physiol 308:C289-96. 2015
    ..We examined splicing of myosin phosphatase regulatory subunit (Mypt1) exon 24 (E24) in relation to smooth muscle myosin heavy chain (Smmhc) and smoothelin (Smtn) alternative exons (..
  7. pmc In vivo roles for myosin phosphatase targeting subunit-1 phosphorylation sites T694 and T852 in bladder smooth muscle contraction
    Cai Ping Chen
    Model Animal Research Center and MOE Key Laboratory of Animal Models of Disease, Nanjing University, Nanjing, China
    J Physiol 593:681-700. 2015
    ..b>MYPT1 is the regulatory subunit of MLCP that biochemically inhibits MLCP activity via T694 or T852 phosphorylation in ..
  8. pmc Redox signaling and splicing dependent change in myosin phosphatase underlie early versus late changes in NO vasodilator reserve in a mouse LPS model of sepsis
    John J Reho
    Department of Medicine, Divisions of Cardiovascular Medicine and Nephrology, University of Maryland, Baltimore, Maryland
    Am J Physiol Heart Circ Physiol 308:H1039-50. 2015
    ..Contractile (myosin and actin) and regulatory [myosin light chain kinase and phosphatase subunits (Mypt1, CPI-17)] mRNAs and proteins were decreased in mesenteric arteries at 24 h concordant with reduced force ..
  9. pmc DAAM1 and DAAM2 are co-required for myocardial maturation and sarcomere assembly
    Rieko Ajima
    Mammalian Development Laboratory, National Institute of Genetics, Mishima 411 8540, Japan
    Dev Biol 408:126-39. 2015
    ..However, deleting Daam1 further disrupted Wnt5a homozygous-null hearts, suggesting that DAAM1 also has Wnt5a-independent roles in cardiac development. ..
  10. pmc A splice variant of the myosin phosphatase regulatory subunit tunes arterial reactivity and suppresses response to salt loading
    John J Reho
    Department of Medicine, Divisions of Cardiovascular Medicine and Nephrology, University of Maryland Baltimore, Baltimore, Maryland
    Am J Physiol Heart Circ Physiol 310:H1715-24. 2016
    ..Variants of MP regulatory subunit Mypt1 are generated by alternative splicing of the 31 nt exon 24 (E24), which, by changing the reading frame, codes for ..
  11. ncbi Identification, characterization, and functional analysis of heart-specific myosin light chain phosphatase small subunit
    T Arimura
    Department of Molecular Pathogenesis, Division of Adult Diseases, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 101 0062, Japan
    J Biol Chem 276:6073-82. 2001
    ..These observations suggest that the heart-specific small subunit hHS-M(21) plays a regulatory role in cardiac muscle contraction by its binding to the large subunit...
  12. doi Characterization of isoform expression and subcellular distribution of MYPT1 in intestinal epithelial cells
    Juan Min Zha
    Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China Cambridge Suda CAM SU Genome Resource Center, Soochow University, Suzhou 215123, China
    Gene 588:1-6. 2016
    ..The activity of the catalytic subunit of MLCP (PP1cδ) toward MLC depends on its regulatory subunit (MYPT1)...
  13. pmc Smoothelin-like 1 protein regulates myosin phosphatase-targeting subunit 1 expression during sexual development and pregnancy
    Beata Lontay
    Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 285:29357-66. 2010
    ..A primary target gene of SMTNL1 in these muscles is myosin phosphatase-targeting subunit 1 (MYPT1)...
  14. doi ROS-mediated downregulation of MYPT1 in smooth muscle cells: a potential mechanism for the aberrant contractility in atherosclerosis
    Jung Chien Cheng
    Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    Lab Invest 93:422-33. 2013
    ..smooth muscle relaxation, is regulated by the phosphorylation of its regulatory subunit, MP targeting subunit 1 (MYPT1)...
  15. pmc Tra2β protein is required for tissue-specific splicing of a smooth muscle myosin phosphatase targeting subunit alternative exon
    Kang Fu
    Department of Medicine Cardiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 287:16575-85. 2012
    Alternative splicing of the smooth muscle myosin phosphatase targeting subunit (Mypt1) exon 23 (E23) is tissue-specific and developmentally regulated and, thus, an attractive model for the study of smooth muscle phenotypic specification...
  16. doi Impaired vascular remodeling in the yolk sac of embryos deficient in ROCK-I and ROCK-II
    Hiroshi Kamijo
    Department of Pharmacology, Kyoto University, Japan
    Genes Cells 16:1012-21. 2011
    ..These results suggest that ROCK isoforms function redundantly during embryogenesis and play a critical role in vascular development...
  17. pmc Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis
    Deqiang Li
    Riley Heart Research Center, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Development 138:303-15. 2011
    ..Biochemical analyses indicate that Daam1 does not regulate cytoskeletal organization through RhoA, Rac1 or Cdc42. Our study highlights a crucial role for Daam1 in regulating the actin cytoskeleton and tissue morphogenesis...
  18. pmc Regulation of basal LC20 phosphorylation by MYPT1 and CPI-17 in murine gastric antrum, gastric fundus, and proximal colon smooth muscles
    B P Bhetwal
    Department of Physiology and Cell Biology, Center of Biomedical Research Excellence, University of Nevada School of Medicine, 1664 N Virginia St, Reno, NV 89557, USA
    Neurogastroenterol Motil 23:e425-36. 2011
    ..Here, we investigate the role of ROK in regulating LC20 phosphorylation and spontaneous contractions of gastric fundus, gastric antrum, and proximal colon smooth muscles...
  19. pmc Altered contractile phenotypes of intestinal smooth muscle in mice deficient in myosin phosphatase target subunit 1
    Wei Qi He
    Model Animal Research Center and MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China
    Gastroenterology 144:1456-65, 1465.e1-5. 2013
    The regulatory subunit of myosin light chain phosphatase, MYPT1, has been proposed to control smooth muscle contractility by regulating phosphorylation of the Ca(2+)-dependent myosin regulatory light chain...
  20. ncbi Green tea polyphenol epigallocatechin-3-gallate signaling pathway through 67-kDa laminin receptor
    Daisuke Umeda
    Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812 8581, Japan
    J Biol Chem 283:3050-8. 2008
    ..activity of EGCG; (ii) through both eEF1A and 67LR, EGCG induces the dephosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) at Thr-696 and activates myosin phosphatase; and (iii) silencing of 67LR, eEF1A, or MYPT1 ..
  21. ncbi Capturing novel mouse genes encoding chromosomal and other nuclear proteins
    P Tate
    MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, Scotland, UK
    J Cell Sci 111:2575-85. 1998
    ..Motifs in two of the isolated genes suggest new links between cellular regulatory mechanisms (ubiquitination and phosphorylation) and mRNA splicing and chromosome structure/function...
  22. ncbi Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase
    Kenta Kobayashi
    Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960 1295, Japan
    J Neurosci 24:3480-8. 2004
    ..These findings indicate that the Rho signaling pathway, primarily through Rho-kinase, plays a crucial role in survival of spinal MNs during embryogenesis, particularly at the early developmental stages...
  23. ncbi Localization of myosin phosphatase target subunit and its mutants
    Yue Wu
    Muscle Biology Group, University of Arizona, Tucson, AZ 85721, USA
    J Muscle Res Cell Motil 26:123-34. 2005
    Transient transfection of NIH3T3 cells with various constructs of myosin phosphatase target subunit (MYPT1) and GFP showed distinct cellular localizations. Constructs containing the N-terminal nuclear localization signals (NLS), i.e...
  24. ncbi The targeted disruption of the MYPT1 gene results in embryonic lethality
    Ryuji Okamoto
    The First Department of Internal Medicine, Mie University School of Medicine, 2 174 Edobashi, Tsu, Mie 514 8507, Japan
    Transgenic Res 14:337-40. 2005
    ..b>MYPT1, a target subunit of smooth and nonmuscle MP, is responsible for activation and regulation of MP...
  25. ncbi Urocortin-induced decrease in Ca2+ sensitivity of contraction in mouse tail arteries is attributable to cAMP-dependent dephosphorylation of MYPT1 and activation of myosin light chain phosphatase
    Lubomir T Lubomirov
    Institute of Vegetative Physiology, University of Cologne, Germany
    Circ Res 98:1159-67. 2006
    ..a approximately 2-fold increase in the phosphorylation of the regulatory subunit of myosin phosphatase (SMPP-1M), MYPT1, at Thr696, which was reversed in arteries relaxed with urocortin. Submaximally (pCa 6...
  26. pmc Myosin phosphatase dephosphorylates HDAC7, controls its nucleocytoplasmic shuttling, and inhibits apoptosis in thymocytes
    Maribel Parra
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco, California 94158, USA
    Genes Dev 21:638-43. 2007
    ..Here, we identify protein phosphatase 1beta (PP1beta) and myosin phosphatase targeting subunit 1 (MYPT1), two components of the myosin phosphatase complex, as HDAC7-associated proteins in ..
  27. ncbi Preserved expression of GLUT4 prevents enhanced agonist-induced vascular reactivity and MYPT1 phosphorylation in hypertensive mouse aorta
    Kevin B Atkins
    Department of Internal Medicine, University of Michigan Medical School, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0676, USA
    Am J Physiol Heart Circ Physiol 293:H402-8. 2007
    ..Phosphorylation of the myosin phosphatase targeting subunit MYPT1 was significantly enhanced in aortas of WT DOCA-salt mice, and this increase was prevented in GLUT4 TG mice...
  28. ncbi Rho-kinase regulates endothelin-1-stimulated IL-6 synthesis via p38 MAP kinase in osteoblasts
    Haruhiko Tokuda
    Department of Clinical Laboratory, National Hospital for Geriatric Medicine, National Center for Geriatrics and Gerontology, Obu, Aichi 474 8511, and Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501 1194, Japan
    Biochem Biophys Res Commun 362:799-804. 2007
    ..These results strongly suggest that Rho-kinase regulates ET-1-stimulated IL-6 synthesis through p38 MAP kinase activation in osteoblasts...
  29. pmc Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak
    Rowena Suriben
    Department of Psychiatry, University of California, San Francisco, San Francisco, California, USA
    Nat Genet 41:977-85. 2009
    ..We conclude that Dact1 contributes to morphogenesis at the primitive streak by regulating Vangl2 upstream of cell adhesion and the PCP pathway...
  30. pmc Myosin light chain kinase activation and calcium sensitization in smooth muscle in vivo
    Yusuke Mizuno
    Dept Physiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    Am J Physiol Cell Physiol 295:C358-64. 2008
    ..may occur through RhoA/Rho-kinase and/or PKC with phosphorylation of myosin phosphatase targeting subunit-1 (MYPT1) and PKC-potentiated phosphatase inhibitor (CPI-17), respectively...
  31. pmc Tra2beta as a novel mediator of vascular smooth muscle diversification
    Supriya Shukla
    Departments of Medicine Cardiology, Case Western Reserve School of Medicine, Cleveland, Ohio 44106, USA
    Circ Res 103:485-92. 2008
    ..We are using the alternative splicing of the MYPT1 E23 exon as a model for the study of smooth muscle diversification into fast and slow contractile phenotypes...
  32. pmc The Rho-Rock-Myosin signaling axis determines cell-cell integrity of self-renewing pluripotent stem cells
    Nicole Harb
    Department of Biochemistry, University of California Riverside, Riverside, California, United States of America
    PLoS ONE 3:e3001. 2008
    ..Although intercellular communications are essential to establish the basis of cell-specific identity, molecular mechanisms underlying intrinsic cell-cell interactions in ES cells at the signaling level remain underexplored...
  33. doi The atypical Guanine-nucleotide exchange factor, dock7, negatively regulates schwann cell differentiation and myelination
    Junji Yamauchi
    Department of Pharmacology and Laboratory Animal Resource Facility, National Research Institute for Child Health and Development, Setagaya, Tokyo 157 8535, Japan
    J Neurosci 31:12579-92. 2011
    ..Collectively, the signaling through Dock7 negatively regulates Schwann cell differentiation and the onset of myelination, demonstrating the unexpected role of Dock7 in the interplay between Schwann cell migration and myelination...
  34. pmc Characterization of a Dchs1 mutant mouse reveals requirements for Dchs1-Fat4 signaling during mammalian development
    Yaopan Mao
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA
    Development 138:947-57. 2011
    ..Our analysis implies that Dchs1 and Fat4 function as a ligand-receptor pair during murine development, and identifies novel requirements for Dchs1-Fat4 signaling in multiple organs...
  35. pmc O-linked beta-N-acetylglucosaminyltransferase substrate specificity is regulated by myosin phosphatase targeting and other interacting proteins
    Win D Cheung
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 283:33935-41. 2008
    ..We demonstrate here that MYPT1 and CARM1 also interact with and target OGT. MYPT1 and CARM1 are substrates of OGT in vitro and in vivo...
  36. pmc Expression of CPI-17 in smooth muscle during embryonic development and in neointimal lesion formation
    Jee In Kim
    Department of Molecular Physiology and Biophysics, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA
    Histochem Cell Biol 132:191-8. 2009
    ..Thus, CPI-17 expression is reversibly controlled in response to the phenotype transition of SM cells that restricts the signal to differentiated SM cells and particular cell types...
  37. doi Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance
    Dou Dou
    Department of Physiology and Pathophysiology, Peking University Health Science Center, 38 Xue Yuan Road, Beijing 100191, People s Republic of China
    Cardiovasc Res 86:151-9. 2010
    ..The present study determines whether the leucine zipper-positive (LZ+) isoform of myosin phosphatase target subunit 1 (MYPT1), a key target protein for PKG actions, is involved in the development of nitrate tolerance.